Nucleic acid extractor suitable for various fluxes

Abstract

The utility model discloses a kind of nucleic acid extractors suitable for multiple flux, comprising: shell;Magnetic bar device, including mounting bracket, lifting frame, lifting drive assembly one, the lifting drive assembly one drives the lifting frame lifting, detachable magnetic bar plate is installed in the lifting frame, the magnetic bar plate quantity is multiple and install different quantity magnetic bar;Lifting device, including lifting frame and lifting drive assembly two, the lifting frame has clamping part, the clamping part is used to clamp magnetic bar cover, the lifting drive assembly two drives the magnetic bar device and the lifting device common lifting.The utility model has beneficial effect in, suitable for 96 well plate, 48 well plate and multiple flux, in different flux nucleic acid extraction work, without frequently changing instrument, greatly reduce manual intervention time and error risk.When needing to switch flux, just replace magnetic bar plate of different magnetic bar quantity, reduce cost, reduce space occupancy.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid extraction technology, specifically to a nucleic acid extractor suitable for multiple throughputs. Background Technology

[0002] A nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically extract nucleic acids from samples. It is widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry, and molecular biology research.

[0003] Existing nucleic acid extractors come in various models, generally suitable for 96-well and 48-well plates. Multiple devices with different throughputs need to be purchased to meet diverse needs. When switching the detection scale, instruments need to be replaced and the process needs to be recalibrated, which significantly increases the time for manual intervention and the risk of errors. There is an urgent need for an extraction device that can be used for multiple throughputs. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model provides a nucleic acid extractor applicable to various throughputs and suitable for processing various models such as 96-well plates and 48-well plates.

[0005] Specifically, this utility model discloses a nucleic acid extractor suitable for various throughputs, including:

[0006] The housing has an internal base, on which a turntable assembly is mounted for rotating the perforated plate;

[0007] A magnetic rod device includes a mounting frame, a lifting frame, and a lifting drive assembly. The mounting frame is fixedly mounted on the base. The lifting drive assembly drives the lifting frame to move up and down. The lifting frame is equipped with detachable magnetic rod plates. There are multiple magnetic rod plates with different numbers of magnetic rods installed on them.

[0008] The lifting device includes a lifting frame and a second lifting drive assembly. The lifting frame has a clamping part for clamping a magnetic rod sleeve. The second lifting drive assembly drives the magnetic rod sleeve and the lifting device to lift together.

[0009] The advantage of adopting the above technical solution is that it is applicable to various throughputs such as 96-well plates and 48-well plates. In nucleic acid extraction work with different throughputs, there is no need to frequently change instruments. When it is necessary to switch the throughput, only the magnetic rod plate with different numbers of magnetic rods needs to be replaced, which reduces costs and space occupation.

[0010] Furthermore, the bottom of the lifting frame has a mounting cavity for accommodating the magnetic rod plate, and protruding blocks are provided on both sides of the mounting cavity, the protruding blocks extending out to fix the magnetic rod plate.

[0011] The advantage of adopting the above technical solution is that by extending the block to fix the magnetic rod plate, the magnetic rod plate can be detached. When performing nucleic acid extraction at different throughputs, only different numbers of magnetic rod plates need to be replaced, thus improving the applicability of the equipment.

[0012] Furthermore, the lifting frame is provided with a mounting groove, the extension block has an extension shaft, the extension shaft is fitted with a spring located in the mounting groove, one end of the extension shaft passes through the side of the lifting frame and has a mounting head.

[0013] The advantage of adopting the above technical solution is that the extension block can be retracted by pulling the installation head, which facilitates the disassembly and installation of the magnetic rod plate. The structure is simple and the operation is convenient.

[0014] Furthermore, the lower surface of the magnetic rod plate is provided with mounting holes, which are arranged in an array, and the magnetic rod is detachably connected to the magnetic rod plate.

[0015] The advantages of adopting the above technical solution are that the number of magnetic rods can be adjusted according to the needs of use, which is convenient. By adjusting the number of magnetic rods, it is possible to flexibly adapt to different throughput nucleic acid extraction requirements. When performing low-throughput nucleic acid extraction, the number of magnetic rods can be reduced; while when performing high-throughput nucleic acid extraction, the number of magnetic rods can be increased to improve extraction efficiency. The magnetic rods and magnetic rod plates are detachably connected, which facilitates the replacement, cleaning and maintenance of the magnetic rods.

[0016] Furthermore, the clamping part includes a clamping head, which is connected to a drive shaft. The clamping heads are symmetrically arranged, and the drive shaft is connected to a rotary drive member. The rotary drive member drives the drive shaft to rotate, thereby causing the clamping heads to rotate.

[0017] The advantage of adopting the above technical solution is that the clamping head is used to clamp and put down the magnetic rod sleeve. The clamping head is controlled to rotate by the rotating shaft. After the clamping head rotates, the entire mechanism moves upward, driving the magnetic rod sleeve located on the turntable assembly to move. The clamping head enables automatic gripping and putting down of the magnetic rod sleeve without manual operation.

[0018] Furthermore, the rotary drive component includes: an electric push rod, a connecting block, a gear, and a rack installed on the rear side of the lifting frame. The output end of the electric push rod is connected to the connecting block, the connecting block is fixed to the rack, the gear is fixedly connected to the drive shaft, and the gear and rack are meshed together.

[0019] The advantage of adopting the above technical solution is that by setting an electric push rod to drive the rack to move, the rack meshes with the gear, driving the gear to rotate, which in turn drives the clamping head to rotate, and the clamping head clamps the magnetic rod sleeve, realizing automatic clamping and unloading.

[0020] Furthermore, the second lifting drive assembly includes a first motor, a synchronous belt, a pulley, and a fixing component. The first motor is mounted on the base to drive the synchronous belt to rotate. The fixing component is fixedly connected to the synchronous belt and to a lifting plate. The lifting plate drives the lifting frame and lifting frame to rise and fall.

[0021] The advantage of adopting the above technical solution is that the lifting component drives the lifting frame to rise and fall, and the use of a synchronous belt ensures the accuracy during the lifting process and meets the needs of use.

[0022] Furthermore, the turntable assembly includes a turntable having multiple receiving openings.

[0023] The advantage of adopting the above technical solution is that the setting of the receiving port can initially fix the orifice plate, ensure that the orifice plate moves smoothly, and prevent liquid from spilling out.

[0024] Furthermore, the housing has an openable top cover, and the housing has a display screen located above the turntable assembly.

[0025] The advantages of adopting the above technical solution are that the openable top cover facilitates operation, the display screen can display the required time, and the display screen is set above the turntable assembly, reducing space occupation.

[0026] Furthermore, the clamping head has an L-shaped cross-section.

[0027] The advantage of adopting the above technical solution is that the bottom of the L-shaped gripper head is used to receive the magnetic rod sleeve, which makes it convenient to grasp and put down. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0029] Figure 1 This is the main axonometric view of the present invention with the shell removed;

[0030] Figure 2 This is an axonometric view of the present invention after the shell has been removed;

[0031] Figure 3 This is a schematic diagram of the overall structure of the nucleic acid extraction instrument applicable to multiple throughputs according to this utility model;

[0032] Figure 4 This is a schematic diagram of the lifting device structure of this utility model;

[0033] Figure 5 This is a schematic diagram of the drive shaft structure of this utility model;

[0034] Figure 6This is a schematic diagram of the lifting frame structure of this utility model.

[0035] The reference numerals used in the attached figures are as follows:

[0036] 1. Housing; 11. Base; 12. Top cover; 2. Turntable assembly; 21. Turntable; 3. Mounting frame; 4. Lifting frame; 41. Magnetic rod plate; 42. Magnetic rod; 43. Extending shaft; 44. Spring; 45. Mounting head; 46. Extending block; 5. Lifting drive assembly one; 6. Lifting frame; 61. Clamping head; 62. Drive shaft; 63. Electric push rod; 64. Connecting block; 65. Gear; 66. Rack; 7. Lifting drive assembly two; 71. First motor; 72. Synchronous belt; 73. Pulley; 74. Fixing component; 8. Magnetic rod sleeve; 9. Liquid baffle. Detailed Implementation

[0037] The present invention will now be described in further detail with reference to the accompanying drawings.

[0038] like Figure 1-6 As shown, this utility model discloses a nucleic acid extractor suitable for various throughputs, comprising:

[0039] The housing 1 has a base 11 inside, on which a turntable assembly 2 is installed to drive the well plate to rotate. The well plate contains the sample to be extracted, and the number of magnetic rods 42 is the same as the number of holes on the well plate.

[0040] The magnetic rod device includes a mounting frame 3, a lifting frame 4, and a lifting drive assembly 5. The mounting frame 3 is fixedly mounted on the base 11. The lifting drive assembly 5 drives the lifting frame 4 to move up and down. The lifting frame 4 is equipped with a detachable magnetic rod plate 41. There are multiple magnetic rod plates 41 and different numbers of magnetic rods 42 are installed on them.

[0041] The lifting device includes a lifting frame 6 and a lifting drive assembly 7. The lifting frame 6 has a clamping part for clamping a magnetic rod sleeve 8. The lifting drive assembly 7 drives the magnetic rod device and the lifting device to rise and fall together.

[0042] The advantages of adopting the above technical solution are that it is applicable to various throughputs such as 96-well plates and 48-well plates. In nucleic acid extraction work at different throughputs, there is no need to frequently change instruments, greatly reducing manual intervention time and error risk. When it is necessary to switch throughputs, only the magnetic rod plate 41 with different numbers of magnetic rods 42 needs to be replaced. Alternatively, different throughput sample extractions can be achieved by removing excess magnetic rods 42, reducing costs and space occupation.

[0043] Furthermore, the bottom of the lifting frame 4 has an installation cavity for accommodating the magnetic rod plate 41. Extending blocks 46 are provided on both sides of the installation cavity. The extending blocks 46 extend to fix the magnetic rod plate 41. The shape of the installation cavity is the same as the shape of the magnetic rod plate 41. After the extending blocks extend, they are located on the lower side of the magnetic rod plate 41. The magnetic rod plate 41 is fixed in the installation cavity by the extending blocks 46.

[0044] The lifting frame 4 is provided with a mounting groove. An extension block 46 can extend or retract from the side of the mounting groove. The extension block 46 has an extension shaft 43, which is integrally threaded with the extension block 46. A spring 44 is fitted onto the extension shaft 43, located within the mounting groove. The other end of the extension shaft 43 passes through the side of the lifting frame 4 and has a mounting head 45. Manually pulling the mounting head 45 compresses the spring 44, causing the extension block 46 to retract into the mounting groove. At this time, the entire mounting cavity is open. After placing the magnetic rod plate 41, releasing the mounting head 45 causes the extension block 46 to extend under the elastic force of the spring 44. Its upper surface contacts the lower surface of the magnetic rod plate 41, providing a fixing function. The extension blocks 46 are symmetrically arranged; one side may have two extension blocks 46. Figure 5 As shown, it ensures stability and is simple and convenient to operate.

[0045] Furthermore, the lower surface of the magnetic rod plate 41 is provided with mounting holes arranged in an array. The magnetic rod 42 is detachably connected to the magnetic rod plate 41. The mounting holes are provided with internal threads, and the top of the magnetic rod 42 is provided with external threads. The number of magnetic rods 42 can be installed according to the usage requirements. Multiple magnetic rod plates 41 can be prepared at the same time, each magnetic rod plate 41 having a different number of magnetic rods 42. Moreover, the positions of the magnetic rod sleeve 8 correspond to each other. When performing different flux detection, the magnetic rod plate 41 with the corresponding magnetic rod 42 can be directly replaced, which is quick and easy to operate.

[0046] The number of magnetic rods 42 can be adjusted according to usage needs, facilitating operation. By adjusting the number of magnetic rods 42, it can flexibly adapt to different throughput nucleic acid extraction requirements. When performing low-throughput nucleic acid extraction, the number of magnetic rods 42 can be reduced to avoid unnecessary resource waste; while when performing high-throughput nucleic acid extraction, the number of magnetic rods 42 can be increased to improve extraction efficiency. Simultaneously, the magnetic rods 42 are detachably connected to the magnetic rod plate 41, facilitating the replacement, cleaning, and maintenance of the magnetic rods 42, ensuring their performance and lifespan.

[0047] In some embodiments, the clamping part includes a clamping head 61, which is connected to a drive shaft 62. The clamping heads 61 are symmetrically arranged. The drive shaft 62 is connected to a rotary drive component, which drives the drive shaft 62 to rotate, thereby causing the clamping heads 61 to rotate. The lifting frame 6 is provided with a groove, and a bearing is installed in the groove. The drive shaft 62 is installed in the bearing and can rotate. The clamping head 61 is fixedly connected to the drive shaft 62 and is located on the lower side of the drive shaft 62. The edge of the magnetic rod sleeve 8 has a protruding edge that facilitates the clamping of the head 61. The cross-section of the clamping head 61 is L-shaped, and the bottom rotates to the lower side of the protruding edge during the clamping process. The lifting frame 6 as a whole lifts and lowers, causing the magnetic rod sleeve 8 to rise.

[0048] Furthermore, the rotary drive component includes: an electric push rod 63, a connecting block 64, a gear 65, and a rack 66 mounted on the rear side of the lifting frame 6. The output end of the electric push rod 63 is connected to the connecting block 64, the connecting block 64 is fixed to the rack 66, the gear 65 is fixedly connected to the drive shaft 62, and the gear 65 and rack 66 are meshed together. The electric push rod 63 rises and falls together with the lifting frame 6. A vertically mounted guide rail is fixedly mounted on the rear side of the lifting frame 6, and a slider is mounted on the guide rail. The connecting block 64 moves vertically along the guide rail. The rack 66 is fixedly mounted on both sides of the connecting block 64. The gear 65 is fixedly mounted to the drive shaft 62. The rack 66 moves up and down, driving the drive shaft 62 to rotate. When it is necessary to grip a product, the gripping head 61 extends to the underside of the magnetic rod sleeve 8, the lifting frame 6 rises, and the magnetic rod sleeve 8 is disengaged from the turntable assembly 2 for the next operation. In addition to the electric push rod, the rotary drive component can also be a cylinder, hydraulic cylinder, etc.

[0049] By setting an electric push rod 63 to drive the rack 66 to move, the rack 66 meshes with the gear 65, driving the gear 65 to rotate, which in turn drives the clamping head 61 to rotate. The clamping head 61 clamps the magnetic rod sleeve 8, realizing automatic clamping and unloading.

[0050] In some implementations, the lifting drive assembly 7 includes a first motor 71, a synchronous belt 72, a pulley 73, and a fixing member 74. The first motor 71 is mounted on the base plate to drive the synchronous belt 72 to rotate. The fixing member 74 is fixedly connected to the synchronous belt 72 and the lifting frame 6, thereby driving the lifting frame 6 to rise and fall. One pulley 73 is mounted on the mounting frame 3, and the other is connected to the output end of the first motor 71, driving the synchronous belt 72 to rotate and the lifting frame 6 to rise and fall. Guide rails and sliders are mounted on both sides of the mounting frame 3. The lifting frame 6 is fixedly connected to the sliders and rises and falls together.

[0051] Furthermore, the lifting drive assembly 5 includes a second motor and a ball screw fixedly installed with the lifting frame 6. The ball cylinder is connected to the output end of the second motor, and the lifting frame 4 is fixedly connected to the movable nut on the ball screw. The second motor drives the ball screw to rotate, further driving the lifting frame 4 to lift independently. At the same time, under the drive of the lifting drive assembly 7, the lifting frame 6 and the lifting frame 4 will also lift as a whole, which is suitable for different usage scenarios.

[0052] In some implementations, the turntable assembly 2 includes a turntable 21 with multiple receiving ports. A motor is installed on the lower side of the turntable 21 to drive the turntable to rotate. Multiple perforated plates can be placed in the multiple receiving ports at one time. At the same time, the turntable 21 can rotate. The bottom of the turntable 21 has a heating module to heat the perforated plates.

[0053] Furthermore, the housing 1 has an openable top cover 12, and the housing 1 has a display screen located above the turntable assembly 2. The display screen is tilted for easy operation.

[0054] The openable top cover 12 facilitates operation, and the display screen can show the required time, etc. At the same time, the display screen is set above the turntable assembly 2, reducing space occupation.

[0055] Furthermore, a baffle plate 9 is provided on the lower side of the magnetic rod sleeve. The baffle plate 9 is connected to a motor and can be rotated by the motor to move to the lower side of the magnetic rod sleeve to prevent the liquid in the magnetic rod sleeve 8 from flowing to the lower perforated plate and causing contamination and magnetic beads to fall off. When replacing the magnetic rod sleeve or when no processing is required, it can be rotated to the side.

[0056] The process begins with the manual placement of the well plate containing the sample to be extracted onto the turntable 21. Simultaneously, the magnetic rod sleeve 8 is positioned appropriately, and the top cover 12 is closed. Mechanical movement and heating then ensure the reaction solution is mixed and fully reacted, leading to cell lysis and the release of nucleic acids. The lifting frame 6 and lifting rack 4 descend. Once the lifting frame 6 is in position, the electric push rod 63 extends, the clamping head 61 rotates, the lifting frame 6 rises, and the clamping head 61 lifts the magnetic rod sleeve 8. The lifting rack 4 then lowers the magnetic rod 42 into the magnetic rod sleeve 8. The magnetic rod 42 and the magnetic rod sleeve 8 descend together to aspirate the magnetic beads within the well plate. The liquid-blocking plate 9 moves to the underside of the magnetic rod sleeve 8. After aspiration, the magnetic beads are transferred to the next well plate via the rotation of the turntable 21. The magnetic rod 42 then rises, separating from the magnetic rod sleeve 8. The magnetic beads lose their magnetic force and fall into the next well plate for nucleic acid elution. After elution, the magnetic rod 42 aspirates the magnetic beads again to the waste bead collection area, completing the extraction process.

[0057] For those skilled in the art, various modifications and improvements can be made without departing from the inventive concept of this utility model, and these modifications and improvements all fall within the protection scope of this utility model.

Claims

1. A nucleic acid extractor suitable for multiple throughputs, characterized in that, include: The housing (1) has a base (11) inside, and the base (11) is equipped with a turntable assembly (2) for driving the perforated plate to rotate; The magnetic rod device includes a mounting frame (3), a lifting frame (4), and a lifting drive assembly (5). The mounting frame (3) is fixedly mounted on the base (11). The lifting drive assembly (5) drives the lifting frame (4) to lift. The lifting frame (4) is equipped with a detachable magnetic rod plate (41). There are multiple magnetic rod plates (41) and different numbers of magnetic rods (42) are installed on them. The lifting device includes a lifting frame (6) and a second lifting drive assembly (7). The lifting frame (6) has a clamping part for clamping a magnetic rod sleeve (8). The second lifting drive assembly (7) drives the magnetic rod device and the lifting device to lift together.

2. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The bottom of the lifting frame (4) has an installation cavity for accommodating the magnetic rod plate (41), and protruding blocks (46) are provided on both sides of the installation cavity. The protruding blocks (46) extend out to fix the magnetic rod plate (41).

3. The nucleic acid extractor applicable to multiple throughputs according to claim 2, characterized in that, The lifting frame (4) is provided with a mounting groove. The extension block (46) has an extension shaft (43). The extension shaft (43) is fitted with a spring (44) located in the mounting groove. One end of the extension shaft (43) passes through the side of the lifting frame (4) and has a mounting head (45).

4. The nucleic acid extractor applicable to multiple throughputs according to claim 3, characterized in that, The lower surface of the magnetic rod plate (41) is provided with mounting holes, which are arranged in an array. The magnetic rod (42) is detachably connected to the magnetic rod plate (41).

5. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The clamping part includes a clamping head (61), which is connected to a drive shaft (62). The clamping heads (61) are symmetrically arranged. The drive shaft (62) is connected to a rotary drive member, which drives the drive shaft (62) to rotate, thereby causing the clamping head (61) to rotate.

6. The nucleic acid extractor applicable to multiple throughputs according to claim 5, characterized in that, The rotary drive component includes: an electric push rod (63), a connecting block (64), a gear (65), and a rack (66) installed on the rear side of the lifting frame (6). The output end of the electric push rod (63) is connected to the connecting block (64), the connecting block (64) is fixed to the rack (66), the gear (65) is fixedly connected to the drive shaft (62), and the gear (65) and the rack (66) are meshed together.

7. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The second lifting drive assembly (7) includes a first motor (71), a synchronous belt (72), a pulley (73), and a fixing member (74). The first motor (71) is mounted on the base to drive the synchronous belt (72) to rotate. The fixing member (74) is fixedly connected to the synchronous belt (72) and to the lifting frame (6), thereby driving the lifting frame (6) to rise and fall.

8. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The turntable assembly (2) includes a turntable (21) having multiple receiving ports.

9. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The housing (1) has an openable top cover (12) and a display screen located above the turntable assembly (2).

10. The nucleic acid extractor applicable to multiple throughputs according to claim 1, characterized in that, The clamping head (61) has an L-shaped cross section.

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