A vortex mixing test tube

Abstract

This application provides a blow-type mixing test tube, relating to the field of test tube technology. It includes a tube body, a top cap mounted at the top of the tube body, and a reaction chamber mounted at the bottom of the tube body. A stirring assembly is provided in the middle of the inner side of the top cap. The stirring assembly includes a pressing head, which is snapped into the middle of the inner side of the top cap. A pipette is provided at the bottom of the pressing head, passing through the top cap and extending into the inner cavity of the tube body. An annular groove is provided at the bottom of the pressing head, and an annular locking block is provided in the middle of the inner side of the top cap. By inserting the locking block into the groove, the pressing head is snapped into the middle of the inner side of the top cap. This invention, by providing a pressing head with a pipette, in conjunction with the reaction module at the bottom of the tube body, allows for thorough mixing of the lysate, magnetic beads, and sample by externally pressing the pressing head in the top cap during use, causing the pipette to draw in and expel the liquid and magnetic beads from the lysis chamber below.

Description

Technical Field

[0001] This utility model relates to the field of test tube technology, specifically a blow-type mixing test tube. Background Technology

[0002] In the field of biological sample testing, especially in the process of nucleic acid extraction, sample pretreatment is usually required, including steps such as mixing, lysis, and washing.

[0003] Traditional methods often use pipettes to repeatedly blow and mix liquids to achieve a stirring effect. However, this operation is usually carried out in an open or semi-open environment, which is inefficient, makes it difficult to generate high concentrations of nucleic acid, and easily generates nucleic acid aerosols, leading to cross-contamination between samples or in the instrument environment, affecting the accuracy and sensitivity of the test results.

[0004] To address the aforementioned problems, we propose a blow-type mixing test tube. Utility Model Content

[0005] To address the problems in the background art, this utility model provides a blow-type mixing test tube.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A blow-type mixing test tube includes a tube body, a top cap installed at the top of the tube body, and a reaction chamber installed at the bottom of the tube body. A stirring assembly is provided in the middle of the inner side of the top cap. The stirring assembly includes a pressing head, which is snapped into the middle of the inner side of the top cap. A pipette is provided at the bottom of the pressing head, which passes through the top cap and extends into the inner cavity of the tube body. An annular groove is provided at the bottom of the pressing head, and an annular locking block is provided in the middle of the inner side of the top cap. By inserting the locking block into the groove, the pressing head is snapped into the middle of the inner side of the top cap.

[0007] Preferably, a reaction module is provided at the top of the reaction chamber, and a liquid injection plate is provided at the top of the reaction module. A sealing plate is provided at the upper middle part of the liquid injection plate. The reaction module, the liquid injection plate and the sealing plate are all located at the bottom of the inner cavity of the tube.

[0008] Preferably, the reaction module includes a first connecting cavity, a first isolation cavity, a cleaning cavity, a second isolation cavity, and a second connecting cavity. The second connecting cavity is located at the top of the reaction cavity and is connected to the reaction cavity. The reaction cavity is a cone shape that is wider at the top and narrower at the bottom.

[0009] Preferably, the injection plate is installed on the top of the reaction module, and the injection plate is provided with a plurality of injection holes communicating with each cavity of the reaction module, and the side ends of the plurality of injection holes are provided with venting grooves.

[0010] Preferably, the injection plate has a vent hole in the middle of its inner side.

[0011] Preferably, the sealing plate is located on top of the injection plate, and the bottom end face of the sealing plate is provided with a cylindrical sealing plug that corresponds to a plurality of injection holes and vent holes.

[0012] Preferably, the bottom end face of the sealing plate is provided with a raised sealing strip.

[0013] Preferably, the top cover and the tube body are connected by threads.

[0014] Compared with the prior art, the beneficial effects of this utility model are: In this scheme, a pressable stirring component is used to draw and blow liquid into a sealed test tube, creating liquid flow and achieving mixing of the sample. This effectively promotes the sample pretreatment process, improves the extraction efficiency and concentration of nucleic acids, and thus enhances the overall detection sensitivity. Furthermore, the entire blowing and mixing process is completed inside a completely sealed test tube, effectively isolating it from the external environment, avoiding nucleic acid aerosol contamination generated during operation, preventing cross-contamination between the sample and the instrument, and ensuring the safety of the detection process and the reliability of the results. In this solution, by opening a venting groove on the side of the injection hole and setting a venting hole in the middle of the injection plate, the liquid can be vented during injection, thereby ensuring the smoothness of liquid injection and effectively solving the problem of liquid not being able to be filled due to the influence of sealing air pressure. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is an exploded view of the present invention; Figure 3 This is a cross-sectional structural diagram of the stirring assembly and the top cover in this utility model; Figure 4 This is a schematic diagram of the reaction module in this utility model; Figure 5 This is a schematic diagram of the structure of the injection plate and the sealing plate in this utility model; Figure 6 This is a top view of the reaction module in this utility model.

[0016] In the diagram: 1. Tube body; 2. Top cover; 21. Locking block; 3. Pressing head; 31. Locking groove; 32. Straw; 4. Injection plate; 41. Injection hole; 42. Vent groove; 43. Vent hole; 5. Sealing plate; 51. Sealing strip; 52. Sealing plug; 6. Reaction module; 61. First connecting cavity; 62. First isolation cavity; 63. Cleaning cavity; 64. Second isolation cavity; 65. Second connecting cavity; 7. Reaction cavity. Detailed Implementation

[0017] The technical solution in this application embodiment is to solve the problems mentioned in the background art, and the overall idea is as follows: Example: Refer to Figure 1 - Figure 6 As shown, a blow-type mixing test tube of this embodiment includes a tube body 1 and a top cover 2 installed at the top of the tube body 1. The top cover 2 and the tube body 1 are connected by threads to enhance the sealing and stability of the connection.

[0018] In some examples, a stirring assembly is provided in the middle of the inner side of the top cover 2. The stirring assembly includes a pressing head 3, which is snapped into the middle of the inner side of the top cover 2. A straw 32 is provided at the bottom of the pressing head 3. The straw 32 passes through the top cover 2 and extends into the inner cavity of the tube body 1. An annular groove 31 is provided at the bottom of the pressing head 3. An annular locking block 21 is provided in the middle of the inner side of the top cover 2. By inserting the locking block 21 into the groove 31, the pressing head 3 is snapped into the middle of the inner side of the top cover 2, so that a stable sealing connection is formed between the pressing head 3 and the top cover 2.

[0019] In some examples, a reaction chamber 7 is installed at the bottom of the tube body 1, a reaction module 6 is provided at the top of the reaction chamber 7, a liquid injection plate 4 is provided at the top of the reaction module 6, and a sealing plate 5 is provided at the upper middle part of the liquid injection plate 4. The reaction module 6, the liquid injection plate 4 and the sealing plate 5 are all located at the bottom of the inner cavity of the tube body 1.

[0020] The sealing plate 5 is used to isolate the reagents inside the tube 1 from the reagents inside the reaction module 6. The reaction module 6 is used to clean the nucleic acid to be tested. The reaction chamber 7 is used to react the nucleic acid with the reaction solution. The reaction module 6 includes a first connecting chamber 61, a first isolation chamber 62, a cleaning chamber 63, a second isolation chamber 64, and a second connecting chamber 65. The second connecting chamber 65 is located at the top of the reaction chamber 7 and is connected to the reaction chamber 7. The reaction chamber 7 is a cone shape that is wider at the top and narrower at the bottom.

[0021] Before testing, silicone oil needs to be added to the first isolation chamber 62 and the second isolation chamber 64, and cleaning fluid needs to be added to the cleaning chamber 63. Both silicone oil and cleaning fluid are used to clean the magnetic beads.

[0022] When in use, this mixing tube can be inserted into the shaftless ball gear. The sample begins to heat up and lyse. The external magnetic beads rotate repeatedly, driving the magnetic beads inside the tube 1 to move. The lysed nucleic acid binds to the magnetic beads. Under the action of the external magnetic beads, the magnetic beads will pass through the first connecting cavity 61, the first isolation cavity 62, the cleaning cavity 63, the second isolation cavity 64, and the second connecting cavity 65 in sequence to complete the cleaning. Furthermore, the paraffin wax at the top of the reaction chamber 7 is melted by temperature control, and magnetic beads carrying nucleic acid enter the reaction chamber 7. Under the action of reagents inside the reaction chamber 7, the magnetic beads separate from the nucleic acid. The magnetic beads reach the bottom of the reaction chamber 7, and the nucleic acid reacts with the reaction solution in the reaction chamber 7. At this time, fluorescence can be collected from the side of the reaction chamber 7 by the detection instrument.

[0023] In some examples, the injection plate 4 is installed on the top of the reaction module 6, and multiple injection holes 41 communicating with each cavity of the reaction module 6 are provided on the injection plate 4. Each injection hole 41 has a venting groove 42 on its side end, and a venting hole 43 is provided in the middle of the inner side of the injection plate 4. The venting groove 42 and the venting hole 43 mainly function to allow air to pass through during injection, so as to avoid the liquid not being able to fill the container due to the influence of the sealing air pressure.

[0024] In some examples, the sealing plate 5 is located on top of the injection plate 4, and the bottom end face of the sealing plate 5 is provided with a cylindrical sealing plug 52 corresponding to multiple injection holes 41 and vent holes 43. By inserting the sealing plug 52 into the corresponding injection hole 41 and vent hole 43, a sealing function is achieved.

[0025] In some examples, the bottom surface of the sealing plate 5 is provided with a raised sealing strip 51, which makes the sealing effect better when the sealing plate 5 and the liquid injection plate 4 are pressed together.

[0026] In some examples, the press head 3 is made of a soft material, such as rubber, latex, polyethylene, polypropylene, or other rubber or polymer materials.

[0027] The working principle of this utility model is as follows: In use, first open the top cover 2 and inject the sample liquid into the inner cavity of the tube body 1 using a pipette. Then seal the tube body 1 by closing the top cover 2. At this time, the mixture can be blown out by the stirring assembly. The specific steps are as follows: the user presses the pressing head 3 on the top cover 2 manually or mechanically, and the lower end of the pipette 32 is immersed in the liquid at the bottom of the tube body 1. When the pressing head 3 is released, the liquid is drawn into the pipette 32. When the pressing head 3 is pressed again, the liquid is blown out. By repeatedly pressing, the pipette 32 draws in and out the liquid and magnetic beads in the lysis chamber below, thereby achieving a thorough mixing of the lysis solution, magnetic beads and sample.

[0028] At the same time, under the action of the external magnet, the magnetic beads will gather directly above the first connecting cavity 61. The magnet will move sequentially along the first connecting cavity 61, the first isolation cavity 62, the cleaning cavity 63, and the second isolation cavity 64 until it moves directly above the second connecting cavity 65. Finally, it will fall into the interior of the reaction cavity 7 through the second connecting cavity 65 to carry out the reaction.

[0029] Throughout the process, tube 1 remains sealed, enabling the liquid to be blown, mixed, and reacted within a closed system, thus effectively preventing contamination during operation.

[0030] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A blow-type mixing test tube, characterized in that, It includes a tube body (1), a top cover (2) installed at the top of the tube body (1), and a reaction chamber (7) installed at the bottom of the tube body (1). A stirring assembly is provided in the middle of the inner side of the top cover (2). The stirring assembly includes a pressing head (3). The pressing head (3) is snapped into the middle of the inner side of the top cover (2). A straw (32) is provided at the bottom of the pressing head (3). The straw (32) passes through the top cover (2) and extends into the inner cavity of the tube body (1). The bottom of the pressing head (3) is provided with an annular slot (31), and the inner middle of the top cover (2) is provided with an annular block (21). By inserting the block (21) into the slot (31), the pressing head (3) is snapped into the inner middle of the top cover (2).

2. The blow-type mixing test tube according to claim 1, characterized in that, The reaction chamber (7) is provided with a reaction module (6) at the top, and a liquid injection plate (4) is provided at the top of the reaction module (6). A sealing plate (5) is provided at the middle of the upper end of the liquid injection plate (4). The reaction module (6), the liquid injection plate (4) and the sealing plate (5) are all located at the bottom of the inner cavity of the tube body (1).

3. A blow-type mixing test tube according to claim 2, characterized in that, The reaction module (6) includes a first connecting cavity (61), a first isolation cavity (62), a cleaning cavity (63), a second isolation cavity (64), and a second connecting cavity (65). The second connecting cavity (65) is located at the top of the reaction cavity (7) and is connected to the reaction cavity (7). The reaction cavity (7) is a cone shape that is wider at the top and narrower at the bottom.

4. A blow-type mixing test tube according to claim 3, characterized in that, The injection plate (4) is installed on the top of the reaction module (6), and multiple injection holes (41) connected to each cavity of the reaction module (6) are provided on the injection plate (4). A venting groove (42) is provided on the side end of each of the multiple injection holes (41).

5. A blow-type mixing test tube according to claim 4, characterized in that, The injection plate (4) has a vent hole (43) in the middle of its inner side.

6. A blow-type mixing test tube according to claim 5, characterized in that, The sealing plate (5) is located on top of the injection plate (4), and the bottom end face of the sealing plate (5) is provided with a cylindrical sealing plug (52) corresponding to multiple injection holes (41) and vent holes (43).

7. A blow-type mixing test tube according to claim 6, characterized in that, The bottom end face of the sealing plate (5) is provided with a raised sealing strip (51).

8. A blow-type mixing test tube according to claim 7, characterized in that, The top cover (2) is threadedly connected to the tube body (1).

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