Incubator for online control of bacterial concentration

Abstract

The utility model relates to the technical field of bacteria culture, and disclose a kind of incubator of online control bacteria concentration, including cabinet, the upper portion of cabinet is provided with controller and power switch, temperature controller is arranged in the controller, air circulation switch, oxygen generator switch and temperature control switch, tray is fixedly installed in the cabinet.This kind of incubator of online control bacteria concentration, bacteria concentration can be monitored and accurately controlled in real time, online control can respond instantly, each period of bacteria is monitored in real time.The utility model saves time, manpower, material resources, reduces pollution, and has wide application range, such as synthetic biology, medicine, pharmacy, industrial fermentation etc.

Description

Technical Field

[0001] This invention relates to the field of bacterial culture technology, specifically to an incubator for online control of bacterial concentration. Background Technology

[0002] Traditional incubators only provide fixed temperature and humidity conditions, making it impossible to monitor the growth of cultured bacteria in real time. If monitoring growth requires manual sampling, bacterial concentration typically needs to be manually sampled and measured in a spectrophotometer, which is inefficient and prone to contamination.

[0003] As per existing technology, Chinese invention patent CN106290162A discloses a device and method for continuously detecting bacterial concentration or growth status. The device includes a detection unit module, a sensor data line and / or a laser control line, a microcontroller control platform, a wireless data communication module, and a terminal data processing device. The device and method described in this invention can continuously measure bacterial concentration under interference-free conditions and other special culture conditions, thereby dynamically monitoring and studying the growth status of bacteria in real time.

[0004] The above-mentioned device still has certain defects: Existing online bacterial concentration detection devices use the principle of laser beams penetrating optical glassware for bacterial culture. When the bacterial concentration is high, the limitations are similar to those of spectrophotometers in detecting bacterial OD values. At high bacterial concentrations, the positive correlation between OD values ​​and absorbance decreases. Online bacterial culture devices can only culture bacteria and cannot detect and control bacterial concentration in real time. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides an incubator for online control of bacterial concentration, used for real-time detection and control of bacterial concentration.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an incubator for online control of bacterial concentration, comprising a chamber body, a controller and a power switch disposed on the top of the chamber body, the controller comprising a temperature controller, a fan circulation switch, an oxygen generation switch and a temperature control switch, a tray fixedly installed inside the chamber body, the tray having micro-vents, a temperature sensor and an oxygen concentration sensor also disposed inside the chamber body, fans disposed at the top and bottom of the chamber body, a heating wire disposed at the top of the chamber body, and a cooling module disposed at the bottom of the chamber body. An oxygen generator is installed at one end of the housing. A vent pipe is fixedly installed at the output end of the oxygen generator and is located above the tray. A vent hole is opened at the bottom of the vent pipe. A sample inlet and outlet hole is opened on one side of the housing. A square tube is also installed on one side of the housing. The square tube is located on the side of the sample inlet and outlet hole and above the tray. Non-contact infrared sensors are installed on both sides of the square tube. The power switch, temperature sensor, oxygen concentration sensor, fan, heating wire, cooling module, oxygen generator and non-contact infrared sensor are all electrically connected to the controller.

[0007] Furthermore, a stirring assembly is installed inside the vent pipe.

[0008] Furthermore, the stirring assembly includes a rotating shaft that passes through the middle of the vent pipe, and both ends of the rotating shaft are rotatably connected to both ends of the housing. A drive fan blade is fixedly installed in the middle of the rotating shaft, and the drive fan blade is located inside the vent pipe. Stirring blades are fixedly installed at both ends of the rotating shaft.

[0009] Furthermore, a pressure pump is connected to the end of the square tube furthest from the housing.

[0010] Furthermore, the end of the pressure pump furthest from the housing is connected to a pipeline.

[0011] Furthermore, a ventilation hole is provided on one side of the box.

[0012] Furthermore, rubber brackets are fixedly installed at the four corners of the bottom of the box.

[0013] Compared with the prior art, the present invention has the following beneficial effects: This type of incubator with online bacterial concentration control enables real-time monitoring and precise control of bacterial concentration. The online control provides immediate response and real-time monitoring of bacteria at various stages. It saves time, manpower, and resources, reduces pollution, and has a wide range of applications, such as synthetic biology, medicine, pharmacy, and industrial fermentation. Attached Figure Description

[0014] Figure 1 This is a front view of the overall structure of this utility model; Figure 2This is a schematic diagram of the overall rear structure of this utility model; Figure 3 This is a partial cross-sectional view of the present invention; Figure 4 for Figure 3 Enlarged view of point A in the middle.

[0015] In the diagram: 1. Chamber; 2. Temperature controller; 3. Air circulation switch; 4. Oxygen generator switch; 5. Temperature control switch; 6. Temperature sensor; 7. Vent hole; 8. Vent pipe; 9. Stirring assembly; 10. Refrigeration module; 11. Fan; 12. Oxygen generator; 13. Tray; 14. Sample inlet / outlet port; 15. Non-contact infrared sensor; 16. Square tube; 17. Power switch; 18. Heating wire; 19. Oxygen concentration sensor; 20. Rubber bracket; 91. Rotating shaft; 92. Drive fan blade; 93. Stirring blade. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0017] Please see Figures 1 to 4 An incubator for online control of bacterial concentration includes a chamber body 1. A controller and a power switch 17 are located on the top of the chamber body 1. The controller contains a temperature controller, a fan circulation switch 3, an oxygen generator switch 4, and a temperature control switch 5. A tray 13 is fixedly installed inside the chamber body 1, and the tray 13 has micro-vents. A temperature sensor 6 and an oxygen concentration sensor 19 are also installed inside the chamber body 1. Fans 11 are installed at both the top and bottom of the chamber body 1. A heating wire 18 is installed at the top of the chamber body 1, and a cooling module 10 is installed at the bottom of the chamber body 1. An oxygen generator 12 is installed at one end of the chamber body 1. The output end is fixedly installed with a vent pipe 8, which is located above the tray 13. A vent hole is opened at the bottom of the vent pipe 8. A sample inlet / outlet hole 14 is opened on one side of the box body 1. A square tube 16 is also provided on one side of the box body 1. The square tube 16 is located on one side of the sample inlet / outlet hole 14 and above the tray 13. Non-contact infrared sensors 15 are provided on both sides of the square tube 16. The power switch 17, temperature sensor 6, oxygen concentration sensor 19, fan 11, heating wire 18, refrigeration module 10, oxygen generator 12 and non-contact infrared sensor 15 are all electrically connected to the controller.

[0018] Specifically, the vent pipe 8 is equipped with a stirring component 9.

[0019] More specifically, the stirring assembly 9 includes a rotating shaft 91 that passes through the middle of the vent pipe 8 and is rotatably connected to the two ends of the housing 1. A transmission fan blade 92 is fixedly installed in the middle of the rotating shaft 91 and is located inside the vent pipe 8. Stirring blades 93 are fixedly installed at both ends of the rotating shaft 91.

[0020] Specifically, a pressure pump is connected to the end of the square tube 16 that is away from the housing 1.

[0021] More specifically, the end of the pressure pump away from housing 1 is connected to a pipeline.

[0022] Specifically, a ventilation hole 7 is provided on one side of the box body 1.

[0023] Specifically, rubber brackets 20 are fixedly installed at the four corners of the bottom of the housing 1. The rubber brackets 20 make the housing 1 more stable during placement and operation.

[0024] When using this online bacterial concentration control incubator, first turn on the power switch 17, air circulation switch 3, and temperature control switch 5 of chamber 1. Set the desired incubation temperature using the temperature controller 2, and use the controller in conjunction with the temperature sensor 6 to control the cooling module 10 and heating wire 18 to maintain a constant temperature within ±0.1℃ of the set temperature. For anaerobic bacterial culture, close the gas inlets and outlets such as the fan 11 as much as possible. Combined with existing anaerobic bacterial culture methods, ensure air pressure balance in chamber 1 through the vent 7. The above operation process is existing technology and will not be elaborated further. For aerobic bacterial culture, turn on the oxygen generator switch 4. The oxygen concentration sensor 19 inside chamber 1 can monitor and adjust the oxygen concentration in real time in conjunction with the controller, providing an aerobic environment. When the oxygen generator 12 is working, oxygen enters the ventilation pipe 8 from the oxygen generator 12 and enters the culture medium through the ventilation hole at the bottom of the ventilation pipe 8. At this time, some oxygen comes into contact with the drive fan blade 92 in the stirring assembly 9, causing it to rotate and drive the rotating shaft 91 to rotate. The rotating shaft 91 then drives the stirring blade 93 to rotate, which disturbs the inside of the culture medium, making the contact between the culture medium and oxygen more uniform, improving the oxygen dissolution efficiency, and making the data obtained by the oxygen concentration sensor 19 more accurate.

[0025] The pressure pump then pumps the inoculated culture medium or droplets generated by the microfluidic chip into the pipeline at a certain flow rate through the square tube 16. The pipeline is connected to the square tube 16 fixed between the two non-contact infrared sensors 15 through the pressure pump. The square tube 16 is made of quartz glass. The droplets can pass through the square tube 16, the pressure pump, and the pipeline, and then enter the chamber 1 through the sample inlet / outlet port 14, and then fall onto the tray 13. This process is the detection process.

[0026] The time it takes for the inoculated culture medium or droplets generated by the microfluidic chip to reach the square tube 16 is calculated by using the flow rate of the pressure pump and the volume of the tubing, as shown in the following formula: t=π*r 2 h / v.

[0027] Where: t is time; r is the inner diameter of the pipe; h is the length of the pipe; v is the flow rate of the pressure pump.

[0028] When the inoculated culture medium or the droplets generated by the microfluidic chip reach the square tube 16, the non-contact infrared sensor 15 transmits the corresponding data to the controller in real time, at which point the pressure pump is turned off.

[0029] When the data transmitted by the non-contact infrared sensor 15 meets the standards or requirements, the pressure pump is started. At this time, the other end of the pipeline can be connected to a pre-prepared sterilized EP tube or sterile syringe for the final collection of droplets to complete the culture of microorganisms. During the process, it is ensured that the droplet flow rate is consistent with the flow rate during detection.

[0030] This invention provides an incubator for online bacterial concentration control, enabling real-time monitoring and precise control of bacterial concentration. The online control provides immediate response and real-time monitoring of bacteria at various stages. This invention saves time, manpower, and resources, reduces pollution, and has a wide range of applications, such as synthetic biology, medicine, pharmacy, and industrial fermentation.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention.

Claims

1. An incubator for online control of bacterial concentration, comprising a chamber (1), characterized in that, A controller and a power switch (17) are installed on the top of the box (1). The controller contains a temperature controller, a wind circulation switch (3), an oxygen generator switch (4), and a temperature control switch (5). A tray (13) is fixedly installed inside the box (1). The tray (13) has micro-vents. A temperature sensor (6) and an oxygen concentration sensor (19) are also installed inside the box (1). Fans (11) are installed at the top and bottom of the box (1). A heating wire (18) is installed at the top of the box (1). A cooling module (10) is installed at the bottom of the box (1). An oxygen generator (12) is installed at one end of the box (1). The output end of the oxygen generator (12) is fixedly installed with... Ventilation pipe (8) is located above tray (13). Ventilation hole is provided at the bottom of ventilation pipe (8). Sample inlet and outlet hole (14) is provided on one side of box body (1). Square tube (16) is also provided on one side of box body (1). Square tube (16) is located on one side of sample inlet and outlet hole (14) and above tray (13). Non-contact infrared sensor (15) is provided on both sides of square tube (16). Power switch (17), temperature sensor (6), oxygen concentration sensor (19), fan (11), heating wire (18), refrigeration module (10), oxygen generator (12) and non-contact infrared sensor (15) are all electrically connected to controller.

2. The incubator for online control of bacterial concentration according to claim 1, characterized in that, The vent pipe (8) is equipped with a stirring component (9).

3. The incubator for online control of bacterial concentration according to claim 2, characterized in that, The stirring assembly (9) includes a rotating shaft (91) that passes through the middle of the vent pipe (8) and is rotatably connected to the two ends of the housing (1). A transmission fan blade (92) is fixedly installed in the middle of the rotating shaft (91) and is located inside the vent pipe (8). Stirring blades (93) are fixedly installed at both ends of the rotating shaft (91).

4. The incubator for online control of bacterial concentration according to claim 1, characterized in that, A pressure pump is connected to the end of the square tube (16) away from the box (1).

5. An incubator for online control of bacterial concentration according to claim 4, characterized in that, The end of the pressure pump away from the housing (1) is connected to a pipeline.

6. The incubator for online control of bacterial concentration according to claim 1, characterized in that, A ventilation hole (7) is provided on one side of the box (1).

7. The incubator for online control of bacterial concentration according to claim 1, characterized in that, Rubber brackets (20) are fixedly installed at the four corners of the bottom of the box (1).

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