A culture device for mucor species for fermented bean curd

Abstract

The utility model discloses a kind of mould spores of fermented bean curd culture devices, including culture shell and control bottom frame, the outside of culture shell is sealingly connected with protective shell, the left and right sides of the protective shell are fixedly connected with connecting frame, the inside of connecting frame is slidably connected with limit block, the outside of limit block is slidably connected with fixed frame, fixed frame is fixedly connected between culture shell, the utility model can limit installation protective shell in the upper end outside of culture shell by the effect of limit block, to form closed structure, and the effect of filter membrane set can be filtered to the outside air and be handled, avoid miscellaneous bacteria into culture medium and affect fermented bean curd edible safety, surface covering layer set simultaneously by the effect of can play protective effect, prevent culture medium surface moisture evaporate too fast, reduce the risk of miscellaneous bacteria pollution simultaneously.

Description

Technical Field

[0001] This utility model relates to the field of microbial fermentation technology, specifically to a cultivation device for Mucor mold strains used in fermented bean curd. Background Technology

[0002] Fermented bean curd, a traditional Chinese fermented soybean product, is loved by consumers for its unique flavor and rich nutrition. Mucor is a key microorganism in the fermentation process of fermented bean curd, and its growth status directly affects the texture, flavor and quality of fermented bean curd. In the production process of fermented bean curd, the fermentation culture of Mucor requires a suitable culture medium to ensure that Mucor can grow rapidly and healthily and form sufficient mycelium, laying a good foundation for the subsequent fermentation of fermented bean curd.

[0003] Existing cultivation devices suffer from several drawbacks. The closed structure of these devices leads to a gradual decrease in internal oxygen levels, affecting the normal fermentation of the *Mucor* strain. Conversely, open cultivation allows gases to carry other microorganisms into the device, compromising the safety of fermented bean curd. Furthermore, existing devices are not conducive to temperature control, resulting in unsuitable fermentation environments for the *Mucor* strain, leading to slow growth and sparse mycelium, which hinders efficient fermentation. Therefore, a new cultivation device for *Mucor* strains used in fermented bean curd is needed to address these issues. Utility Model Content

[0004] The purpose of this invention is to provide a cultivation device for Mucor mold strains used in fermented bean curd, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cultivation device for Rhizopus spp. for fermented bean curd, comprising a cultivation shell and a control bottom frame, wherein a protective shell is sealed to the outside of the cultivation shell, and connecting frames are fixedly connected to both the left and right sides of the protective shell, a limiting block is slidably connected to the inside of the connecting frame, and a fixing frame is slidably connected to the outside of the limiting block, and the fixing frame is fixedly connected to the cultivation shell. A control base frame is fixedly connected to the lower end of the culture shell. An air pump is fixedly connected to the inside of the control base frame near the middle position. A gas heat conduction box is sealed to the rear end of the air pump's outlet pipe. A heating tube is fixedly connected inside the gas heat conduction box. An air inlet pipe is sealed to the upper end of the heating tube. The air inlet pipe penetrates the lower end wall of the culture shell.

[0006] Preferably, the lower inner end of the culture shell is provided with a basic nutrient layer, a functional adjustment layer is provided above the basic nutrient layer, and a surface covering layer is provided above the functional adjustment layer. The surface covering layer is set as a sterile agar layer.

[0007] Preferably, an insulation layer is fixedly connected to the inner side of the protective shell, and a vent is provided on the outer side wall of the protective shell, with a filter membrane inside the vent.

[0008] Preferably, a spring sheet is fixedly connected inside the connecting frame, the spring sheet is fixedly connected to the limiting block, and a pressing block is provided on the outer side of the limiting block, the pressing block is slidably connected to the fixed frame.

[0009] Preferably, the front end of the control base frame is provided with a control panel, and a PLC controller is fixedly connected to the front inside of the control base frame. The PLC controller is electrically connected to the control panel and to the air pump. A temperature probe is electrically connected to the upper front end of the PLC controller, and the temperature probe is fixedly connected to the culture shell.

[0010] Preferably, a filter frame is sealed to the right end of the air inlet pipe on the right side of the air pump, and an air inlet filter screen is fixedly connected to the inner right end of the filter frame.

[0011] Preferably, the protective shell is made of food-grade polypropylene.

[0012] Compared with the prior art, the beneficial effects of this utility model are: 1. This device for cultivating Rhizopus spores for fermented bean curd uses a limiting block to limit the protective shell to be installed on the upper outer side of the cultivation shell, thereby forming a closed structure. The filter membrane can guide and filter the outside air to prevent miscellaneous bacteria from entering the culture medium and affecting the safety of fermented bean curd. At the same time, the surface covering layer can protect the culture medium from excessive evaporation of moisture and reduce the risk of contamination by miscellaneous bacteria.

[0013] 2. This device for cultivating Rhizopus spores for fermented bean curd uses an air pump to introduce external gas between the cultivation shell and the protective shell to ensure sufficient oxygen inside. A temperature probe senses the temperature between the cultivation shell and the protective shell, and a PLC controller automatically controls the operation of the heating element to heat the incoming gas. This allows the Rhizopus spores to ferment in a suitable environment, thereby improving the fermentation efficiency of the Rhizopus spores. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the installation structure of this utility model; Figure 2 This is a sectional view of the installation structure of this utility model; Figure 3 This is a cross-sectional view of the installation structure of the culture shell and protective shell of this utility model; Figure 4This is a sectional view of the installation structure of the connecting frame and the fixing frame of this utility model; Figure 5 This is a schematic diagram of the installation structure of the control base frame of this utility model.

[0015] In the diagram: 1. Culture shell; 2. Protective shell; 3. Connecting frame; 4. Limiting block; 5. Fixing frame; 6. Control base frame; 7. Air pump; 8. Gas heat conduction box; 9. Heating tube; 10. Air inlet pipe; 11. Basic nutrient layer; 12. Functional adjustment layer; 13. Surface covering layer; 14. Insulation layer; 15. Filter membrane; 16. Spring plate; 17. Pressing block; 18. Control panel; 19. PLC controller; 20. Temperature probe; 21. Filter frame; 22. Air inlet filter. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Example 1

[0018] Please refer to Figure 1-5 As shown, this utility model provides a cultivation device for Rhizopus spp. for fermented bean curd, including a cultivation shell 1 and a control bottom frame 6. A protective shell 2 is sealed to the outside of the cultivation shell 1. A connecting frame 3 is fixedly connected to both the left and right sides of the protective shell 2. A limiting block 4 is slidably connected to the inside of the connecting frame 3. A fixing frame 5 is slidably connected to the outside of the limiting block 4. The fixing frame 5 is fixedly connected to the cultivation shell 1. The limiting block 4 can limit the connection frame 3 and the fixing frame 5 to each other, thereby limiting the installation of the protective shell 2. A control base frame 6 is fixedly connected to the lower end of the culture shell 1. An air pump 7 is fixedly connected to the inside of the control base frame 6 near the middle. A gas heat conduction box 8 is sealed to the rear end of the air outlet pipe of the air pump 7. A heating pipe 9 is fixedly connected inside the gas heat conduction box 8. An air inlet pipe 10 is sealed to the upper end of the heating pipe 9. The air inlet pipe 10 penetrates the lower wall of the culture shell 1. By starting the air pump 7, external air can enter between the culture shell 1 and the protective shell 2 through the air inlet pipe 10 to ensure sufficient oxygen content. The gas heat conduction box 8 and the heating pipe 9 can heat the incoming gas to ensure that the Mucor mold can ferment in a suitable environment and improve the fermentation efficiency of the Mucor mold.

[0019] Specifically, the lower end of the culture shell 1 is provided with a basic nutrient layer 11, a functional regulation layer 12 is provided above the basic nutrient layer 11, and a surface covering layer 13 is provided above the functional regulation layer 12. The surface covering layer 13 is set as a sterile agar layer. The soybean meal, wheat bran, corn flour and other ingredients in the basic nutrient layer 11 can provide rich carbon sources, nitrogen sources and minerals to meet the basic nutritional needs of Mucor growth. The tofu residue, soybean peptone and other ingredients in the functional regulation layer 12 further supplement nutrition. B vitamins promote the metabolism of Mucor, and calcium carbonate regulates the pH value of the culture medium to create a suitable environment for Mucor growth. The sterile agar layer of the surface covering layer 13 can play a protective role, preventing the surface moisture of the culture medium from evaporating too quickly and reducing the risk of contamination by other microorganisms.

[0020] Specifically, an insulation layer 14 is fixedly connected to the inner side of the protective shell 2, and a vent is provided on the outer side wall of the protective shell 2. A filter membrane 15 is provided inside the vent. The vent and the filter membrane 15 can effectively filter bacteria and dust in the air, prevent bacteria from contaminating the culture medium, and ensure normal air circulation.

[0021] Specifically, a spring sheet 16 is fixedly connected inside the connecting frame 3. The spring sheet 16 is fixedly connected to the limiting block 4. A pressing block 17 is provided on the outer side of the limiting block 4. The pressing block 17 is slidably connected to the fixed frame 5. By pressing the pressing block 17, the limiting block 4 can move inward to the connecting frame 3, thereby compressing the spring sheet 16, so that the protective shell 2 can be quickly removed to facilitate the extraction of the mold inside.

[0022] Specifically, a control panel 18 is provided on the front end of the control base frame 6. A PLC controller 19 is fixedly connected to the front inside the control base frame 6. The PLC controller 19 is electrically connected to the control panel 18 and to the air pump 7. A temperature probe 20 is electrically connected to the upper front end of the PLC controller 19 and is fixedly connected to the culture shell 1. The control panel 18 facilitates the control of electronic equipment, and the PLC controller 19 and the temperature probe 20 enable timely monitoring of temperature changes and timely control of the heating tube 9 to ensure the stability of the fermentation temperature of the Mucor mold and avoid the impact of air temperature changes on the fermentation of the Mucor mold.

[0023] Specifically, the right end of the air pump 7's right air intake pipe is sealed with a filter frame 21, and the right end of the inner side of the filter frame 21 is fixedly connected with an air intake filter 22, which can filter the outside air through the filter frame 21 and the air intake filter 22 before introducing it, thus preventing the air from carrying bacteria into the system.

[0024] Specifically, the protective shell 2 is made of food-grade polypropylene. Food-grade polypropylene has good corrosion resistance, high temperature resistance and safety, and will not contaminate the culture medium, ensuring the safety of fermented bean curd for consumption.

[0025] Working Principle: This invention relates to a cultivation device for *Rhizopus* spores used in fermented bean curd. During fermentation, a basic nutrient layer 11 is first placed inside the lower part of the cultivation shell 1. A functional adjustment layer 12 is then laid on top of the basic nutrient layer 11, followed by a surface covering layer 13. This three-layer structure, with its well-matched raw materials, provides sufficient carbon, nitrogen, minerals, and vitamins to meet the nutritional needs of *Rhizopus* at different growth stages, effectively promoting its growth and reproduction, increasing mycelial yield and quality, and providing high-quality spores for subsequent fermentation. A protective shell 2 is then placed over the upper outer part of the cultivation shell 1, and a limiting block 4 ensures the protective shell remains in place. During fermentation, a filter membrane 15 effectively filters airborne bacteria and dust, preventing contamination of the culture medium. The culture medium is prepared by starting the air pump 7 using the control panel 18, allowing external air to pass through the filter frame 21 and the air inlet filter 22 before entering the gas heat conduction box 8. The air then enters the space between the culture shell 1 and the protective shell 2 through the air inlet pipe 10, ensuring normal air circulation. At the same time, the temperature probe 20 monitors the temperature between the culture shell 1 and the protective shell 2. If the temperature exceeds or falls below the suitable temperature for the Rhizopus spores, the information is directly transmitted to the PLC controller 19, which directly starts the heating tube 9 to control the temperature of the incoming gas. This ensures that the Rhizopus spores are fermenting in a suitable environment, improving the fermentation efficiency. After fermentation, pressing the pressing block 17 moves the limiting block 4 inward to the connecting frame 3, compressing the spring sheet 16 and allowing the protective shell 2 to be quickly removed for easy extraction of the Rhizopus spores inside.

[0026] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0027] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A cultivation device for Mucor mold strains used in fermented bean curd, comprising a cultivation shell (1) and a control base frame (6), characterized in that: The outer side of the culture shell (1) is sealed with a protective shell (2). The left and right sides of the protective shell (2) are fixedly connected with connecting frames (3). The inner side of the connecting frame (3) is slidably connected with a limit block (4). The outer side of the limit block (4) is slidably connected with a fixed frame (5). The fixed frame (5) is fixedly connected to the culture shell (1). The lower end of the culture shell (1) is fixedly connected to a control bottom frame (6). An air pump (7) is fixedly connected to the inside of the control bottom frame (6) near the middle position. The rear end of the air outlet pipe of the air pump (7) is sealed to a gas heat conduction box (8). A heating tube (9) is fixedly connected inside the gas heat conduction box (8). An air inlet pipe (10) is sealed to the upper end of the heating tube (9). The air inlet pipe (10) penetrates the lower end wall of the culture shell (1).

2. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: The lower end of the culture shell (1) is provided with a basic nutrient layer (11), a functional adjustment layer (12) is provided above the basic nutrient layer (11), and a surface covering layer (13) is provided above the functional adjustment layer (12). The surface covering layer (13) is set as a sterile agar layer.

3. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: The inner side of the protective shell (2) is fixedly connected with a heat insulation layer (14), and the outer side wall of the protective shell (2) is provided with a vent hole, and the inside of the vent hole is provided with a filter membrane (15).

4. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: A spring sheet (16) is fixedly connected inside the connecting frame (3). The spring sheet (16) is fixedly connected to the limiting block (4). The outer side of the limiting block (4) is provided with a pressing block (17). The pressing block (17) is slidably connected to the fixed frame (5).

5. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: The front end of the control base frame (6) is provided with a control panel (18). A PLC controller (19) is fixedly connected to the front inside of the control base frame (6). The PLC controller (19) is electrically connected to the control panel (18). The PLC controller (19) is electrically connected to the air pump (7). A temperature probe (20) is electrically connected to the upper front end of the PLC controller (19). The temperature probe (20) is fixedly connected to the culture shell (1).

6. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: The air pump (7) has a filter frame (21) sealed to the right end of the air inlet pipe on the right side, and an air inlet filter (22) is fixedly connected to the right end of the inner side of the filter frame (21).

7. The cultivation device for Mucor mold strain for fermented bean curd according to claim 1, characterized in that: The protective shell (2) is made of food-grade polypropylene.

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