A 10l disposable reactor support device for cell culture

Abstract

The utility model discloses a 10L disposable reactor support device for cell culture, it includes jar body, magnetic drive module and weighing base. The jar body adopts 304 stainless steel material, including inner cylinder, jacket cylinder and jacket partition, the left and right sides of the lower part of inner cylinder are welded with two arc steel sheets, for the precise fit with the appearance of disposable stirring bag, the jacket cylinder is sleeved on the outside of inner cylinder, and the upper and lower ends are welded with inner cylinder sealing plate to form jacket chamber, the jacket partition is a plurality of annular stainless steel sheets, a notch is formed on each jacket partition, and the notches are distributed at intervals of 180 degrees in the circumferential direction, and the plurality of jacket partitions are segment spot welded on the inner wall of the jacket cylinder and arranged at intervals with the outer wall of the inner cylinder. The 10L disposable reactor support device for cell culture provided by the utility model solves the technical problems of insufficient heat preservation and temperature control performance, poor temperature conduction and bag body fit in the technical field of bioreactor equipment.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor equipment technology, specifically to a 10L disposable reactor support device for cell culture. Background Technology

[0002] In the fields of biopharmaceutical and stem cell research, the 10L disposable reactor is the core equipment for in vitro stem cell culture. The structural design of its supporting device directly determines the stability of the culture environment, the efficiency of temperature conduction, and the ease of operation, which has a key impact on the maintenance of stem cell activity and culture quality.

[0003] The mainstream 10L disposable reactor support devices currently on the market have significant defects in structural design and performance, making it difficult to meet the technical requirements for high-quality stem cell culture. Specific problems are as follows: 1. Insufficient heat preservation and temperature control performance: The outer shell of existing devices is generally made of Teflon material, which only has basic heat insulation capabilities and lacks a closed heat preservation structure design. Heat is lost quickly and cannot maintain the stable temperature environment required for culture for a long time. At the same time, the heating method relies on electric blanket heating, which has low heat conduction efficiency and uneven temperature distribution. Not only is the heating speed slow and it is difficult to quickly reach the set temperature for stem cell culture, but local temperature fluctuations are also prone to occur, which directly affect the activity and proliferation efficiency of stem cells.

[0004] 2. Poor temperature conduction and bag fit: The inner cylinder structure of existing devices is mostly a single cylindrical design, which is poorly adapted to the bottom shape of the disposable stirring bag. The small contact area between the two causes the heat of the temperature control medium transferred by the jacket to be unable to be efficiently conducted to the inside of the bag, further aggravating the problem of uneven temperature distribution. In addition, the bag is prone to deformation due to the lack of precise support, which affects the stability of the cultivation process. Utility Model Content

[0005] The purpose of this invention is to overcome the above-mentioned technical deficiencies and to provide a 10L disposable reactor support device for cell culture, which solves the technical problems of insufficient heat preservation and temperature control performance, poor temperature conduction and bag fit in the field of bioreactor equipment technology.

[0006] To achieve the above-mentioned technical objectives, the present invention provides a 10L disposable reactor support device for cell culture, comprising: Tank body, magnetic drive module, and weighing base; The tank body is made of 304 stainless steel and includes an inner cylinder, a jacketed cylinder, and jacket partitions. Two arc-shaped steel plates are welded to the lower left and right sides of the inner cylinder to precisely fit the shape of the disposable mixing bag. The jacketed cylinder is fitted onto the outside of the inner cylinder, and inner cylinder sealing plates are welded to its upper and lower ends to form a jacketed chamber. The jacket partitions are multiple annular stainless steel plates, each with notches spaced 180° apart circumferentially. The multiple jacket partitions are spot-welded to the inner wall of the jacketed cylinder in sections and spaced apart from the outer wall of the inner cylinder. The notches on each jacket partition form a connected flow channel to guide the heat exchange medium in the jacketed chamber to circulate. The tank also includes an upper conical insulation shell and a bottom sealing plate of the insulation shell; the upper end of the upper conical insulation shell is a conical stainless steel plate with an open structure, which is welded to the bottom sealing plate of the insulation shell to form a closed insulation space around the outside of the jacket cylinder and filled with insulation material pearl cotton. The magnetic drive module includes a magnet and a drive source, which drives the stirring paddle inside the disposable mixing bag to rotate through a magnetic field. The weighing base includes a weighing module for real-time monitoring of the weight changes of the culture medium inside the tank.

[0007] Compared with the prior art, the beneficial effects of this utility model include: 1. Excellent thermal insulation performance: The tank body is made of 304 stainless steel with an upper conical thermal insulation shell and a bottom sealing plate, forming a closed and high-strength thermal insulation structure. At the same time, the thermal insulation space is filled with insulating material pearl cotton, which can effectively reduce heat loss and maintain a stable temperature environment inside the tank for a long time, meeting the stringent requirements of stem cell culture for temperature stability.

[0008] 2. Strong temperature conduction performance: The arc-shaped steel plate welded to the arc-shaped inner cylinder can precisely fit the bottom of the disposable culture bag, greatly improving the support area and stability of the disposable culture bag, effectively preventing deformation of the disposable culture bag, and at the same time, it is more conducive to temperature conduction, making the temperature inside the disposable culture bag more uniform. Attached Figure Description

[0009] Figure 1 This is a three-dimensional structural diagram of the 10L disposable reactor support device for cell culture provided by this utility model; Figure 2 This utility model provides a tank body and a schematic diagram of its cross-sectional structure; Figure 3 This is an exploded structural diagram of the magnetic drive module provided by this utility model; Figure 4 This is a schematic diagram of the magnetic drive module and its cross-sectional structure provided by this utility model; Figure 5 This is a cross-sectional structural diagram of the magnet cover support provided by this utility model.

[0010] The labels in the attached diagram are explained as follows: Tank body 1, magnetic drive module 2, weighing base 3, inner cylinder 1-1, jacket cylinder 1-2, jacket partition 1-3, arc-shaped steel plate 1-4, inner cylinder sealing plate 1-5, jacket chamber 1-6, upper conical insulation shell 1-7, insulation shell bottom sealing plate 1-8, magnet 2-1, drive source 2-2, magnet cover 2-3, magnet fixing block 2-4, mounting recess 2-41, magnet seat 2-5, magnet cover support 2-6, U-shaped hole 2-61, fixing plate 2-7, lifting guide rod 2-8, foot 3-1, upper bracket 1-9, lower bracket 1-10, insertion sleeve 1-11, exhaust valve 1-12, one-way valve 1-13, internal and external threaded elbow 1-14, chuck external threaded elbow 1-15, support leg 1-16. Detailed Implementation

[0011] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0012] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0013] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0014] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 This embodiment provides a 10L disposable reactor support device for cell culture, including a tank 1, a magnetic drive module 2, and a weighing base 3.

[0015] Furthermore, the tank body is made of 304 stainless steel and includes an inner cylinder 1-1, a jacketed cylinder 1-2, and a jacketed partition 1-3. Two arc-shaped steel plates 1-4 are welded to the lower left and right sides of the inner cylinder 1-1 for precise fitting with the shape of the disposable mixing bag. Specifically, the arc-shaped steel plates 1-4 welded to the inner cylinder 1-1 can precisely fit the bottom of the disposable culture bag, significantly increasing the support area and stability of the disposable culture bag, effectively preventing deformation of the disposable culture bag, and also facilitating heat conduction, resulting in a more uniform temperature within the disposable culture bag.

[0016] Furthermore, the jacketed cylinder 1-2 is fitted onto the outside of the inner cylinder 1-1, and the upper and lower ends are welded with inner cylinder sealing plates 1-5 to form a jacketed chamber 1-6. Specifically, the jacketed chamber 1-6 is used for a temperature-controlled medium (such as hot water, cold water, or heat transfer oil), and the temperature of the culture environment inside the tank 1 is controlled by the circulation of the temperature-controlled medium. The jacket partition 1-3 is composed of multiple annular stainless steel plates, each with notches spaced 180° circumferentially. Multiple jacket partitions 1-3 are spot-welded to the inner wall of the jacketed cylinder 1-2 in sections and spaced apart from the outer wall of the inner cylinder 1-1. The notches on each jacket partition 1-3 form a connected flow channel to guide the circulation of the heat exchange medium in the jacketed chamber 1-6, avoiding local temperature dead zones and improving the uniformity and stability of temperature control.

[0017] Furthermore, the tank body 1 also includes an upper conical insulation shell 1-7 and a bottom sealing plate 1-8 of the insulation shell. The upper end of the upper conical insulation shell 1-7 is a conical stainless steel plate with an open structure. It is welded to the bottom sealing plate 1-8 of the insulation shell to form a closed insulation space around the outside of the jacket cylinder 1-2 and is filled with insulation material pearl cotton to reduce heat loss from the bottom of the tank body 1 and improve the overall insulation effect.

[0018] Furthermore, the magnetic drive module 2 includes a magnet 2-1 and a drive source 2-2, which drive the stirring paddle inside the disposable stirring bag to rotate through a magnetic field.

[0019] Furthermore, the magnetic drive module 2 also includes a magnet cover 2-3 made of Teflon material, a magnet fixing block 2-4, a magnet base 2-5 made of 304 stainless steel, and a magnet cover support 2-6; the magnet cover 2-3 has a three-layer cylindrical structure on the outside and hollowed-out steps on the inside. The bottom cylinder has a threaded hole and is fixedly connected to the magnet cover support 2-6 by bolts.

[0020] Specifically, the bottom cylindrical part of the magnet cover 2-3 has four countersunk holes for hexagonal socket head cap screws, which are used to fix the magnet cover support 2-6. The magnet cover 2-3 is used to protect the magnet 2-1, prevent corrosion of the magnet 2-1, magnet fixing block 2-4 and other components, and reduce the interference of the magnetic field to the outside world.

[0021] Furthermore, the magnet fixing block 2-4 has multiple mounting recesses 2-41 for fixing the magnet, and a central hole is opened in the center, and multiple mounting holes are opened on the periphery. It is fixedly connected to the magnet base 2-5 by bolts; the lower end of the magnet base 2-5 has an inner hole that cooperates with the output shaft of the drive source 2-2, and is fixedly connected by a set screw in the threaded hole of the inner hole wall.

[0022] Specifically, there are six mounting recesses 2-41, which can accommodate and limit the magnets 2-1.

[0023] Specifically, the mounting holes on the periphery of the magnet fixing block 2-4 are countersunk holes for hexagonal socket head cap screws, which are used to fix the magnet to the magnet base 2-5, ensuring that the magnet 2-1 is securely installed. The magnet fixing block 2-4 has a central hole to avoid obstructing other components.

[0024] Furthermore, the central opening of the magnet cover support 2-6 facilitates the installation of the magnet base 2-5, and the inner wall is provided with a U-shaped hole 2-61 to adjust the installation position of the magnet base 2-5 with the set screw. It is fixedly connected to the fixing plate 2-7 at the fixed end of the drive source 2-2 by the inner ring bolt, and the outer ring thread is fixedly connected to the magnet cover 2-3 by bolts. The fixing plate 2-7 is also provided with a guide rod mounting hole on its periphery and is provided with an internal thread, and a lifting guide rod 2-8 is threadedly connected to it.

[0025] Specifically, the magnet cover support 2-6 has two rings of bolt mounting holes. The inner ring of bolt mounting holes is used to fix the magnet cover support 2-6 to the fixing plate 2-7, and the outer ring of bolt mounting holes is used to bolt and fix the magnet cover 2-3. The fixing plate 2-7 is generally square in shape, with a central hole and a cylindrical step. This step is used to position the magnet cover support 2-6 and ensure that the installation position of the magnet cover support 2-6 is accurate. The fixing plate 2-7 has a ring of 8 screw holes, of which 4 are used to install the magnet cover support 2-6 and 4 are used to install the drive source 2-2 on the bottom surface. The fixing plate 2-7 has 4 countersunk holes at the four corners of the square, with a conical countersunk end, for installing the lifting guide rod 2-8.

[0026] Preferably, the drive source 2-2 is a 400W servo motor, which has the characteristics of stable speed and uniform torque output, providing power to the magnetic drive module 2. By controlling the speed of the drive source 2-2, the stirring speed can be precisely adjusted to meet the stirring needs of different stem cell culture stages.

[0027] Furthermore, the lifting guide rod 2-8 is made of 304 stainless steel, with an internal threaded hole at the upper end along the direction of the lifting guide rod 2-8, and an external thread at the lower end; the lower two sides of the lifting guide rod 2-8 are machined with flat surfaces, which facilitates the use of a wrench to rotate the lifting guide rod 2-8. By adjusting the installation height of the lifting guide rod 2-8, the overall height of the magnetic drive module 2 can be adjusted to ensure precise fit with the tank body 1.

[0028] Furthermore, the weighing base 3 includes a weighing module for real-time monitoring of the weight change of the culture medium inside the tank 1. The weighing base 3 has adjustable feet 3-1 at its four corners, with silicone pads attached to the bottom of each foot 3-1. The height and level of the base can be adjusted by rotating the feet 3-1 to ensure the entire device is placed stably. The silicone pads also provide anti-slip and shock absorption, reducing the impact of external vibrations on the culture process. It should be further noted that the weighing base 3, including the weighing module, is existing technology and will not be described in detail further.

[0029] Furthermore, the tank body 1 also includes an upper support 1-9 and a lower support 1-10 made of steel pipe, an insert sleeve 1-11 made of round steel, an exhaust valve 1-12, a one-way valve 1-13, an internal and external threaded elbow 1-14, and a chuck external threaded elbow 1-15.

[0030] Furthermore, both ends of the upper support 1-9 are bent at 90°. The upper support 1-9 is made of steel pipe, and the ends are sealed with pipe caps to suspend the silicone tubes of the culture bag, ensuring that the silicone tubes are stably placed during the culture process and avoiding the impact of the culture operation on the tubes due to shaking. The peripheral side of the insertion sleeve 1-11 is provided with threaded holes. The end of the upper support 1-9 is inserted into the center hole of the insertion sleeve 1-11, and the upper support 1-9 is fixed by screwing in bolts through the threaded holes of the insertion sleeve 1-11. It is also fixed to the tank body 1 by welding with a round bar to ensure the stability of the upper support 1-9 after installation and to prevent the upper support 1-9 from shifting during use.

[0031] Specifically, the insertion sleeve 1-11 is made of round steel, and the upper bracket 1-9 is fixed by screwing screws into the threaded holes around the insertion sleeve 1-11.

[0032] Furthermore, the lower support 1-10 is made of steel pipe. The middle section of the lower support 1-10 is bent according to the curvature of the insulation shell, and both ends are bent at 90° to support the pH electrode and dissolved oxygen electrode, ensuring that the electrode installation position is fixed and the detection data is accurate.

[0033] Furthermore, a plurality of support legs 1-16 are welded to the bottom of the tank body 1, and circular foot plates with symmetrical circular holes are welded to the bottom of the support legs 1-16. The tank body 1 is fixed to the weighing base 3 by bolts passing through the circular holes.

[0034] In this embodiment, three support legs 1-16 are provided to support the entire tank body 1.

[0035] In this embodiment, the exhaust valve 1-12, the one-way valve 1-13, the internal and external threaded elbow 1-14, and the chuck external threaded elbow 1-15 are all made of 304 stainless steel and are industry standard parts. The exhaust valve 1-12 is used to discharge excess gas in the tank 1, the one-way valve 1-13 prevents external gas or liquid from flowing back in, and the internal and external threaded elbow 1-14 and the chuck external threaded elbow 1-15 are used to connect pipelines to realize the transportation of culture medium or temperature control medium.

[0036] This utility model also provides an installation method for the device, as follows: S1. Rotate the foot 3-1 to adjust it to a suitable height to ensure that the weighing base 3 remains stable.

[0037] S2. Install the lifting guide rod 2-8 on the upper end of the weighing base 3 to initially fix the lifting guide rod 2-8. At the same time, the lifting guide rod 2-8 can be adjusted to adjust the magnetic drive module 2 fixed to the lifting guide rod 2-8.

[0038] S3. Install the drive power supply on the bottom surface of the fixing plate 2-7 with bolts, and then install the magnet cover support 2-6 on the top surface of the fixing plate 2-7 with bolts; insert the magnet into the mounting recess 2-41 of the magnet fixing block 2-4, and then connect the magnet fixing block 2-4 to the magnet seat 2-5 with bolts; insert the motor output shaft into the inner hole at the lower end of the magnet seat 2-5, fix it with the set screw on the inner hole wall, and adjust the position of the magnet seat 2-5 through the U-shaped hole 2-61 on the side of the magnet cover support 2-6 to ensure accurate alignment; fix the magnet cover 2-3 on the magnet cover support 2-6 with a threaded connection; finally, connect the fixing plate 2-7 to the lifting guide rod 2-8, insert the four lifting guide rods 2-8 into the countersunk holes at the four corners of the bottom surface of the fixing plate 2-7 for positioning, and fix them with bolts from the back of the fixing plate 2-7 to complete the installation of the magnetic drive module 2.

[0039] S4. Install the standard components such as the exhaust valve 1-12, one-way valve 1-13, internal and external threaded elbow 1-14, and chuck external threaded elbow 1-15 onto the inlet and outlet ports of the jacket cylinder 1-2, ensuring a sealed connection without leakage; place the tank 1 on the weighing base 3, aligning the circular holes of the circular foot plates of the support legs 1-16 with the positioning holes on the weighing base 3, and fix the tank 1 with bolts; insert the upper bracket 1-9 and lower bracket 1-10 into the corresponding insertion sleeves 1-11, tighten the screws by hand, and fix the upper bracket 1-9 and lower bracket 1-10, completing the installation of the entire device.

[0040] Working principle: The 10L disposable reactor support device for cell culture provided by this utility model includes a tank 1, a magnetic drive module 2, and a weighing base 3. The tank body 1 is made of 304 stainless steel and includes an inner cylinder 1-1, a jacketed cylinder 1-2, and a jacketed partition 1-3. Two arc-shaped steel plates 1-4 are welded to the lower left and right sides of the inner cylinder 1-1 to precisely fit the shape of the disposable mixing bag. The jacketed cylinder 1-2 is fitted onto the outside of the inner cylinder 1-1, and inner cylinder sealing plates 1-5 are welded to its upper and lower ends to form a jacketed chamber 1-6. The jacketed partition 1-3 is composed of multiple annular stainless steel plates, each with notches spaced 180° apart circumferentially. Multiple jacketed partitions 1-3 are spot-welded to the inner wall of the jacketed cylinder 1-2 in sections and spaced apart from the outer wall of the inner cylinder 1-1. The notches on each jacketed partition 1-3 form a connected flow channel to guide the heat exchange medium in the jacketed chamber 1-6 to circulate, avoid local temperature dead zones, and improve the uniformity and stability of temperature control.

[0041] The tank body 1 also includes an upper conical insulation shell 1-7 and a bottom sealing plate 1-8. The upper end of the upper conical insulation shell 1-7 is a conical stainless steel plate with an open structure. It is welded to the bottom sealing plate 1-8 to form a closed insulation space around the outside of the jacket cylinder 1-2 and is filled with insulation material pearl cotton. The magnetic drive module 2 includes a magnet 2-1 and a drive source 2-2, which drives the stirring paddle in the disposable stirring bag to rotate through the magnetic field. The weighing base 3 includes a weighing module for real-time monitoring of the weight change of the culture medium inside the tank body 1.

[0042] Specifically, first, rotate the foot 3-1 to adjust it to a suitable height to ensure that the weighing base 3 remains stable. Then, install the lifting guide rod 2-8 on the upper end of the weighing base 3 to initially fix the lifting guide rod 2-8. At the same time, the lifting guide rod 2-8 can be adjusted to adjust the magnetic drive module 2 that is fixed to the lifting guide rod 2-8.

[0043] Next, the drive power supply is bolted to the bottom surface of the fixing plate 2-7, and the magnet cover support 2-6 is bolted to the top surface of the fixing plate 2-7. The magnet is inserted into the mounting recess 2-41 of the magnet fixing block 2-4, and then the magnet fixing block 2-4 is bolted to the magnet seat 2-5. The motor output shaft is inserted into the inner hole at the lower end of the magnet seat 2-5 and fixed by the set screw on the inner hole wall. The position of the magnet seat 2-5 is adjusted by the U-shaped hole 2-61 on the side of the magnet cover support 2-6 to ensure accurate alignment. The magnet cover 2-3 is fixed to the magnet cover support 2-6 by threaded connection. Finally, the fixing plate 2-7 is connected to the lifting guide rod 2-8, and the four lifting guide rods 2-8 are respectively inserted into the countersunk holes at the four corners of the bottom surface of the fixing plate 2-7 for positioning. They are then fixed from the back of the fixing plate 2-7 with bolts to complete the installation of the magnetic drive module 2.

[0044] Finally, install the standard components such as the exhaust valve 1-12, one-way valve 1-13, internal and external threaded elbow 1-14, and chuck external threaded elbow 1-15 onto the inlet and outlet ports of the jacket cylinder 1-2, ensuring a sealed connection without leakage; place the tank 1 on the weighing base 3, aligning the circular holes of the circular foot plates of the support legs 1-16 with the positioning holes on the weighing base 3, and fix the tank 1 with bolts; insert the upper bracket 1-9 and lower bracket 1-10 into the corresponding insertion sleeves 1-11, and tighten the screws by hand to fix the upper bracket 1-9 and lower bracket 1-10, completing the installation of the entire device.

[0045] The specific embodiments of this utility model described above do not constitute a limitation on the scope of protection of this utility model. Any other corresponding changes and modifications made based on the technical concept of this utility model should be included within the scope of protection of the claims of this utility model.

Claims

1. A 10L disposable reactor support device for cell culture, characterized in that, Includes the tank body, magnetic drive module, and weighing base; The tank body is made of 304 stainless steel and includes an inner cylinder, a jacketed cylinder, and jacket partitions. Two arc-shaped steel plates are welded to the lower left and right sides of the inner cylinder to precisely fit the shape of the disposable mixing bag. The jacketed cylinder is fitted onto the outside of the inner cylinder, and inner cylinder sealing plates are welded to its upper and lower ends to form a jacketed chamber. The jacket partitions are multiple annular stainless steel plates, each with notches spaced 180° apart circumferentially. The multiple jacket partitions are spot-welded to the inner wall of the jacketed cylinder in sections and spaced apart from the outer wall of the inner cylinder. The notches on each jacket partition form a connected flow channel to guide the heat exchange medium in the jacketed chamber to circulate. The tank also includes an upper conical insulation shell and a bottom sealing plate of the insulation shell; the upper end of the upper conical insulation shell is a conical stainless steel plate with an open structure, which is welded to the bottom sealing plate of the insulation shell to form a closed insulation space around the outside of the jacket cylinder and filled with insulation material pearl cotton. The magnetic drive module includes a magnet and a drive source, which drives the stirring paddle inside the disposable mixing bag to rotate through a magnetic field. The weighing base includes a weighing module for real-time monitoring of the weight changes of the culture medium inside the tank.

2. The 10L disposable reactor support device for cell culture according to claim 1, characterized in that, The magnetic drive module also includes a magnet cover and a magnet fixing block made of Teflon material, a magnet base and a magnet cover support made of 304 stainless steel; the magnet cover has a three-layer cylindrical structure on the outside and hollowed-out steps on the inside. The bottom cylinder has a threaded hole and is fixedly connected to the magnet cover support by bolts.

3. The 10L disposable reactor support device for cell culture according to claim 2, characterized in that, The magnet fixing block has multiple mounting recesses for fixing the magnet, and a central hole is opened in the center, and multiple mounting holes are opened on the periphery. It is fixedly connected to the magnet base by bolts. The lower end of the magnet base has an inner hole that cooperates with the output shaft of the drive source and is fixedly connected by a set screw in the threaded hole of the inner hole wall.

4. The 10L disposable reactor support device for cell culture according to claim 3, characterized in that, The central opening of the magnet cover support facilitates the installation of the magnet base. The inner wall has a U-shaped hole to adjust the installation position of the magnet base's set screw. It is fixedly connected to the fixing plate of the drive source fixing end by the inner ring bolt, and the outer ring thread is fixedly connected to the magnet cover bolt. The fixing plate also has guide rod mounting holes on its periphery and is provided with internal threads, and a lifting guide rod is threadedly connected to it.

5. The 10L disposable reactor support device for cell culture according to claim 4, characterized in that, The tank also includes an upper support and a lower support made of steel pipe, a tubing sleeve made of round steel, an exhaust valve, a one-way valve, internal and external threaded elbows, and a chuck external threaded elbow; both ends of the upper support are bent at 90° and sealed with pipe caps for suspending the silicone tubes of the culture bags; the tubing sleeve has threaded holes on its periphery, and the end of the upper support is inserted into the center hole of the tubing sleeve. The upper support is fixed by bolts screwed into the threaded holes of the tubing sleeve and fixed to the tank by welding with round bars.

6. The 10L disposable reactor support device for cell culture according to claim 5, characterized in that, The middle section of the lower support is bent according to the curvature of the insulation shell, and both ends are bent at 90° to support the pH electrode and dissolved oxygen electrode.

7. The 10L disposable reactor support device for cell culture according to claim 6, characterized in that, The bottom of the tank is welded with multiple support legs, and the bottom of each support leg is welded with a circular foot plate with symmetrical circular holes. The tank is fixed to the weighing base by bolts passing through the circular holes.

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