Online automatic reaction cell for VFA determination

Abstract

The utility model provides a kind of for VFA determination online automatic reaction pool, including reaction pool body and with it corresponding reaction pool cover, the reaction pool body includes neck and taper portion, the neck is straight pipe shape, the neck forms the taper portion of spinal canal by tapering down, emptying hole is arranged at the taper tip of the taper portion, overflow hole is arranged in the lateral wall of the neck, acid hole, alkali hole, air hole, sample inlet hole and pH meter hole that communicate the inside of the reaction pool body are arranged on the reaction pool cover, the reaction pool cover is airtight connection with the reaction pool body.This reaction pool is compact, portable, improves the overall performance and service life of equipment, solves the problem of easy overflow, ensures the accuracy of detection result, improves the sealing performance of equipment, prevents liquid leakage and sample loss, meets the detection needs of different types and quantities, improves the practicability of equipment, improves analysis efficiency, reduces the consumption of diluent.

Description

Technical Field

[0001] This utility model belongs to the field of water quality testing, specifically relating to an online automatic reaction tank for VFA determination. Background Technology

[0002] In clinical medical diagnostics and food safety testing, immunoassay technology is widely used for the diagnosis of various diseases and the detection of contaminants. Traditional immunoassay methods, such as enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay, and immunochromatography, while still valuable in specific applications, suffer from drawbacks including complex operation, long processing times, high reagent consumption, and high costs. In recent years, the application of microfluidic chip technology has provided new insights into bioanalysis and immunoassay. This technology integrates sample preparation, reaction, separation, and detection into a chip of only a few square centimeters, enabling automated analysis through precise control of the fluid within the microchannels.

[0003] However, existing microfluidic chip technology still faces some challenges in application. For example, the processing precision requirements for reagent cards are extremely high, and the performance of different batches of products is difficult to control, which limits its widespread adoption in large-scale production and application. In addition, in practical use, the cleaning of the reaction cell and the discharge of samples also affect analytical efficiency. Therefore, developing an online automated assay reaction cell that is simple in structure, functionally integrated, and easy to clean and discharge has become a current research hotspot.

[0004] In specific applications, such as online water quality monitoring and analysis, cleaning is one of the most time-consuming steps in the analysis. Existing microfluidic chip technology has limitations in terms of reagent card processing precision, and the performance of different batches is difficult to control, restricting its widespread adoption in large-scale production and application. Furthermore, the existing reaction cell structure design is not optimal, resulting in a cumbersome cleaning process, consuming large amounts of diluent, and requiring manual operation, thus impacting analytical efficiency. Moreover, existing reaction cells have problems with sample discharge, affecting analytical efficiency. For example, some reaction cell designs lack an effective venting mechanism, making it difficult to discharge waste liquid in a timely manner, potentially affecting the accuracy of subsequent analyses. Existing reaction cell lid designs lack functional integration and diverse orifice layouts, making it difficult to meet the needs of different types and quantities of detection. For example, many reaction cell lids lack dedicated pH meter holes, limiting the ability to monitor the reaction process in real time. Existing reaction cells have insufficient overflow prevention design, failing to effectively prevent spilled liquid from affecting the test results, potentially limiting the accuracy of the analytical results. Therefore, there is an urgent need for a reaction cell design that enables rapid cleaning and automated operation to improve analytical efficiency and accuracy. Utility Model Content

[0005] Existing technologies suffer from unreasonable reaction cell structural designs, resulting in cumbersome cleaning processes, inconvenient sample discharge, and low functional integration. Therefore, to address these issues, this invention provides an online automated reaction cell for VFA determination.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: An online automated reaction cell for VFA determination includes a reaction cell body and a reaction cell cover adapted thereto. The reaction tank body includes a neck and a conical section. The neck is a straight tube, and the neck narrows downward to form a conical section. A drain hole is provided at the tip of the conical section, and an overflow hole is provided on the side wall of the neck. The reaction tank cover is provided with an acid addition hole, an alkali addition hole, a vent hole, a sample inlet hole, and a pH meter hole that connect to the interior of the reaction tank body. The reaction tank cover is airtightly connected to the reaction tank body.

[0007] Preferably, the inner diameter of the neck is 42~78mm.

[0008] Preferably, the height of the cone is 56~104mm.

[0009] Preferably, the inner diameter of the vent hole is 4.2~7.8mm.

[0010] Preferably, an exhaust guide pipe is also provided at the exhaust hole, and the exhaust guide pipe is integrally formed with the cone portion.

[0011] Preferably, the wall thickness of the reaction tank body is 1.4~2.6mm.

[0012] Preferably, a sealing ring is provided between the reaction tank cover and the reaction tank body to ensure a tight seal between them.

[0013] Preferably, the reaction tank cover and the reaction tank body are connected by a snap-fit ​​connection.

[0014] Preferably, the vent is located at the center of the reaction tank cover.

[0015] Preferably, the centers of the acid addition hole, the alkali addition hole, the vent hole, and the pH meter hole are on the same straight line.

[0016] Preferably, the aperture of the acid addition orifice is 2.1~3.9mm, the aperture of the alkali addition orifice is 2.1~3.9mm, the aperture of the vent is 2.8~5.2mm, the aperture of the sample inlet is 4.2~7.8mm, and the aperture of the pH meter orifice is 5.6~10.4mm.

[0017] Preferably, the inner wall of the reaction tank body is provided with an anti-corrosion coating to prevent the reaction tank from being corroded by the sample.

[0018] Preferably, the acid addition port, alkali addition port, vent port, sample inlet port, and pH meter port are all equipped with sealing plugs to prevent liquid leakage. A pH meter electrode can be installed inside the pH meter port for real-time monitoring of pH changes during the reaction process.

[0019] Compared with the prior art, this utility model provides an online automatic reaction cell for VFA determination, which has the following advantages: (1) The reaction tank body adopts a structural design that combines straight pipe and conical pipe, which realizes the compactness and lightness of the reaction tank and improves the overall performance and service life of the equipment.

[0020] (2) The present invention provides an overflow hole on the side of the neck of the reaction tank body as an overflow and venting channel, which effectively solves the problem of easy overflow of existing reaction tanks and ensures the accuracy of the test results.

[0021] (3) The reaction tank cover and the reaction tank body are connected by a sealing ring to form an integral structure, which improves the sealing performance of the equipment and prevents liquid leakage and sample loss.

[0022] (4) The surface of the reaction tank cover integrates multiple functional holes such as acid addition hole, alkali addition hole, vent hole, sample inlet hole and pH meter hole, which meet the detection needs of different types and quantities and improve the practicality of the equipment.

[0023] (5) The bottom hole design of the reaction tank body facilitates the rapid discharge of waste liquid, improves the analysis efficiency, and reduces the consumption of diluent. Attached Figure Description

[0024] Figure 1 This is a three-dimensional structural diagram of the reaction tank body in this utility model.

[0025] Figure 2 This is a schematic diagram of the structure of the reaction tank cover in this utility model.

[0026] Among them, 1 is the reaction tank body, 2 is the reaction tank cover, 3 is the acid addition hole, 4 is the alkali addition hole, 5 is the vent hole, 6 is the sample inlet hole, 7 is the pH meter hole, 8 is the overflow hole, and 9 is the drain guide pipe. Detailed Implementation

[0027] Example 1 An online automated reaction cell for VFA determination includes a reaction cell body and a reaction cell cover adapted thereto. The reaction cell body includes a neck and a conical portion. The neck is a straight tube, and the conical portion is tapered downwards to form a conical tube. An vent hole is provided at the tip of the conical portion, and an overflow hole is provided on the side wall of the neck. The reaction cell cover is provided with an acid addition hole, an alkali addition hole, a vent hole, a sample inlet hole, and a pH meter hole that communicate with the interior of the reaction cell body. The reaction cell cover is airtightly connected to the reaction cell body.

[0028] The inner diameter of the neck is 60 mm.

[0029] The height of the cone is 80mm.

[0030] The inner diameter of the vent hole is 6mm.

[0031] An venting guide pipe is also provided at the venting hole, and the venting guide pipe is integrally formed with the cone.

[0032] The wall thickness of the reaction tank body is 2 mm.

[0033] A sealing ring is provided between the reaction tank cover and the reaction tank body to ensure a tight seal between them.

[0034] The reaction tank cover is connected to the reaction tank body by a snap-fit ​​connection.

[0035] The vent is located at the center of the reaction tank cover.

[0036] The centers of the acid addition hole, the alkali addition hole, the vent hole, and the pH meter hole are on the same straight line.

[0037] The aperture of the acid addition port is 3 mm, the aperture of the alkali addition port is 3 mm, the aperture of the vent port is 4 mm, the aperture of the sample inlet port is 6 mm, and the aperture of the pH meter port is 8 mm.

[0038] The acid addition port, alkali addition port, vent port, sample inlet port, and pH meter port are all equipped with sealing plugs to prevent liquid leakage. A pH meter electrode can be installed inside the pH meter port for real-time monitoring of pH changes during the reaction process.

[0039] The reaction vessel body is made of stainless steel, while the reaction vessel cover is made of polycarbonate. The inner wall of the reaction vessel body is coated with a polytetrafluoroethylene (PTFE) anti-corrosion coating. The acid and alkali filling holes of the reaction vessel cover are each fitted with corrosion-resistant rubber stoppers for sealing and sample addition.

[0040] A pH meter electrode is installed inside the pH meter orifice. The electrode has a sensitivity of 0.01 pH units and a measurement range of 0-14 pH. A stirrer (IKA RCT basic model) is installed inside the reaction tank, with an adjustable stirring speed between 100-3000 rpm.

[0041] This reaction cell can be used for online automated determination of volatile organic compounds (VOCs) in blood samples. The specific steps are as follows: 1) Disassemble the reaction cell body and reaction cell cover, remove the stirrer inside the reaction cell body, and add the blood sample into the sample loading cell of the reaction cell body through the sample inlet.

[0042] 2) Snap the reaction tank cover onto the reaction tank body and ensure that the sealing ring is properly sealed.

[0043] 3) Turn on the mixer and set the mixing speed to 2000 rpm.

[0044] 4) Add appropriate amounts of acidic and alkaline solutions to the acid addition hole and the alkali addition hole, respectively, to promote the volatilization of VOCs.

[0045] 5) Monitor the pH changes in the reaction tank using a pH meter until the pH stabilizes at 7.4±0.1.

[0046] 6) Stop the agitator, open the overflow hole and the drain pipe, and discard the waste liquid in the reaction tank.

[0047] 7) Replace the cleaning solution in the reaction tank with fresh solution, and repeat steps 5-6 until a stable baseline is obtained after three cleaning cycles.

[0048] At this point, the reaction chamber can be used to detect the VOC content in blood samples. During detection, calibration is performed according to a known standard curve and baseline values, and the VOC concentration in the sample is calculated.

Claims

1. An online automated reaction cell for VFA determination, characterized in that, This includes the reaction tank body and the corresponding reaction tank cover. The reaction tank body includes a neck and a conical section. The neck is a straight tube, and the neck narrows downward to form a conical section. A drain hole is provided at the tip of the conical section, and an overflow hole is provided on the side wall of the neck. The reaction tank cover is provided with an acid addition hole, an alkali addition hole, a vent hole, a sample inlet hole, and a pH meter hole that connect to the interior of the reaction tank body. The reaction tank cover is airtightly connected to the reaction tank body.

2. The online automated reaction cell for VFA determination according to claim 1, characterized in that, An venting guide pipe is also provided at the venting hole, and the venting guide pipe is integrally formed with the cone.

3. The online automated reaction cell for VFA determination according to claim 1, characterized in that, A sealing ring is provided between the reaction tank cover and the reaction tank body.

4. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The reaction tank cover is connected to the reaction tank body by a snap-fit ​​connection.

5. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The vent is located at the center of the reaction tank cover.

6. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The centers of the acid addition hole, the alkali addition hole, the vent hole, and the pH meter hole are on the same straight line.

7. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The inner diameter of the neck is 42~78mm, and the height of the cone is 56~104mm.

8. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The aperture of the acid addition orifice is 2.1~3.9mm, the aperture of the alkali addition orifice is 2.1~3.9mm, the aperture of the vent is 2.8~5.2mm, the aperture of the sample inlet is 4.2~7.8mm, and the aperture of the pH meter orifice is 5.6~10.4mm.

9. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The inner wall of the reaction tank body is provided with an anti-corrosion coating.

10. The online automated reaction cell for VFA determination according to claim 1, characterized in that, The acid addition port, the alkali addition port, the vent, the sample inlet, and the pH meter port are all equipped with sealing plugs.

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