A micro fluorescent collection, analysis and detection structure

Abstract

The utility model provides a kind of structure of miniature fluorescence collection and analysis detection belongs to fluorescence collection detection technical field, including host computer shell, the inside installation of host computer shell has movement mechanism, detection mechanism is installed on the movement mechanism, mainboard is carried on the detection mechanism, integrated control system is carried on the mainboard.The utility model solves the problem that most of the current market is medium and large-sized desktop detection instrument, and most of them are plug and play, need to determine reagent card position positioning detection, the efficiency is lower, and the instrument with high degree of automation is mostly large instrument, cannot be carried, and the overall cost is higher.

Description

Technical Field

[0001] This invention belongs to the field of fluorescence acquisition and detection technology, specifically a structure for miniature fluorescence acquisition, analysis and detection. Background Technology

[0002] A fluorescence immunoassay analyzer is an instrument capable of measuring and analyzing trace amounts of substances. It converts the structure and properties of substances into measurable spectral signals. It features high sensitivity, strong specificity, speed, accuracy, and ease of operation, and is unaffected by the properties of the substances being analyzed. It is one of the most important analytical and detection technologies today.

[0003] Most of the existing market instruments are medium to large benchtop testing instruments, and most of them are plug-and-play. They require the reagent card to be positioned first, which is inefficient. On the other hand, highly automated instruments are mostly large instruments that cannot be carried around and have a high overall cost.

[0004] In summary, existing miniature fluorescence acquisition and analysis detection technologies suffer from drawbacks such as being unportable, high cost, and the need to pre-determine and position the reagent card, resulting in low efficiency. This invention provides a miniature fluorescence acquisition and analysis detection structure that significantly reduces usage costs, improves portability, and enhances detection efficiency. Utility Model Content

[0005] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0006] Given the following technical problems in the existing technology: how to design a slope protection brick that can be fixed and connected to each other through splicing, so as to form an overall stable structure and adapt to the complex changes in the natural environment.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a structure for miniature fluorescence acquisition, analysis and detection, including a host housing, a motion mechanism installed inside the host housing, a detection mechanism installed on the motion mechanism, a motherboard mounted on the detection mechanism, and an integrated control system mounted on the motherboard.

[0008] As a preferred technical solution for a structure for miniature fluorescence acquisition, analysis and detection, the main unit housing includes an upper shell and a lower shell, which are connected by a pin. The bottom of the lower shell is provided with a slot, and the other end of the lower shell is provided with a charging interface. The lower shell and the upper shell are provided with positioning sleeves inside, and the positioning sleeves are installed on the inner wall of the lower shell by bolts.

[0009] As a preferred technical solution for a structure for miniature fluorescence acquisition, analysis and detection, the motion mechanism includes a support fixed in the lower shell, a drive motor fixed to one end of the support, a lead screw installed at the output end of the drive motor, the lead screw passing through the support and rotatably connected to its other wall, a guide rod fixed parallel to the side of the lead screw on the support, a slider slidably installed on the guide rod, and a gripper with internal threads integrally formed on the slider, the inner wall of the gripper engaging with the lead screw.

[0010] As a preferred technical solution for a micro-fluorescence acquisition and analysis detection structure, the detection mechanism includes an optical path fixing seat mounted on the upper surface of the slider. The surface of the optical path fixing seat is provided with three independent limiting grooves, in which a lamp plate, a filter, and an amplification circuit board are respectively adapted and installed. An optical path cover plate is installed on the upper surface of the optical path fixing seat by bolts.

[0011] As a preferred technical solution for a micro-fluorescence acquisition, analysis and detection structure, the integrated control system includes a processor module, a power supply module, a device driver module, and a signal conditioning module. The processor module is electrically connected to the power supply module, the device driver module, and the signal conditioning module. The device driver module includes a motor driver chip, a Bluetooth module, and a QR code scanning interface. The signal conditioning module includes a second-order RC filter circuit and an instrumentation amplifier.

[0012] As a preferred technical solution for a micro-fluorescence acquisition and analysis detection structure, the amplification circuit board integrates an AD8605 low-noise operational amplifier.

[0013] As a preferred technical solution for a micro-fluorescence acquisition, analysis and detection structure, the processor module is an STM32L476RGT6 and the Bluetooth module uses the BLE5.0 protocol.

[0014] As a preferred technical solution for a micro-fluorescence acquisition and analysis detection structure, the power module includes a TP4056 charging management chip and an RT9193LDO.

[0015] The beneficial effects of this utility model are:

[0016] 1. This utility model features a compact overall structure with a main unit shell and motion mechanism, making it easy to disassemble and maintain. It can be operated with one hand and is suitable for real-time detection scenarios such as outdoor emergency response and primary healthcare. It does not require pre-positioning and improves overall efficiency.

[0017] 2. This utility model, through the integration of a control system and a testing mechanism, is plug-and-play, greatly improving overall testing efficiency and significantly reducing usage efficiency. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0019] Figure 1 This is a schematic diagram of the overall front structure of an embodiment of the present utility model;

[0020] Figure 2 This is a schematic diagram of the overall rear structure of an embodiment of the present utility model;

[0021] Figure 3 This is a schematic diagram of the internal structure of an embodiment of the present utility model;

[0022] Figure 4 This is a schematic diagram of the motion mechanism structure according to an embodiment of the present utility model;

[0023] Figure 5 This is a schematic diagram of the detection mechanism structure according to an embodiment of the present utility model;

[0024] Figure 6 This is a schematic diagram of the module connection structure of an embodiment of the present utility model;

[0025] Reference numerals: 100, Main unit casing; 101, Upper casing; 102, Lower casing; 103, Slot; 104, Charging interface; 105, Positioning sleeve; 200, Motion mechanism; 201, Support; 202, Drive motor; 203, Lead screw; 204, Guide rod; 205, Slider; 206, Gripper; 300, Detection mechanism; 301, Optical path fixing seat; 302, Lamp board; 303, Filter; 304, Amplification circuit board; 305, Optical path cover plate; 400, Integrated control system; 401, Processor module; 402, Power supply module; 403, Device drive module; 404, Signal conditioning module; 405, Motor drive chip; 406, Bluetooth module; 407, QR code scanning interface. Detailed Implementation

[0026] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0027] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0028] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0029] Secondly, this utility model is described in detail with reference to the schematic diagrams. When describing the embodiments of this utility model, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not adhering to the usual scale. Furthermore, the schematic diagrams are merely examples and should not limit the scope of protection of this utility model. In addition, actual manufacturing should include the three-dimensional spatial dimensions of length, width, and depth.

[0030] Example 1

[0031] Reference Figures 1-6 This embodiment provides a structure for miniature fluorescence acquisition, analysis and detection, including a main unit housing 100, a motion mechanism 200 installed inside the main unit housing 100, a detection mechanism 300 installed on the motion mechanism 200, a motherboard mounted on the detection mechanism 300, and an integrated control system 400 mounted on the motherboard.

[0032] The main unit casing 100 serves as the core of the entire device for carrying and protecting it. Inside, a motion mechanism 200 is installed. This motion mechanism 200 provides precise reciprocating drive for the detection process. A detection mechanism 300 is installed on the motion mechanism 200, which is responsible for the excitation and acquisition of fluorescent signals. The detection mechanism 300 is equipped with a motherboard, which serves as the circuit connection hub. An integrated control system 400 is installed on the motherboard to coordinate the motion control, signal processing, and data interaction of the device.

[0033] Specifically, the main unit housing 100 includes an upper housing 101 and a lower housing 102. The upper housing 101 and the lower housing 102 are connected by a pin. The bottom of the lower housing 102 is provided with a slot 103. The other end of the lower housing 102 located in the slot 103 is provided with a charging interface 104. The lower housing 102 and the upper housing 101 are provided with a positioning sleeve 105. The positioning sleeve 105 is installed on the inner wall of the lower housing 102 by bolts.

[0034] The upper shell 101 and the lower shell 102 are connected by pins. This connection method ensures both ease of assembly and structural strength of the main unit shell 100, facilitating quick disassembly during later maintenance. The slot 103 at the bottom of the lower shell 102 is sized to match the standard fluorescent immunoassay reagent card, guiding the reagent card for precise insertion and limiting its lateral displacement. The charging interface 104 adopts the Type-C standard, with its VCC, GND, and data pins connected to the IN+, IN-, and detection pins of the TP4056 charging management chip in the power module 402 via wires, enabling external power input and charging status detection, and efficiently charging the device's built-in lithium battery. The positioning sleeve 105 is bolted to the inner wall of the lower shell 102, providing rigid support for the motion mechanism 200 and the main board, ensuring that internal components do not shift when the device moves or vibrates, thus guaranteeing detection accuracy.

[0035] Specifically, the motion mechanism 200 includes a support 201 fixed in the lower shell 102. A drive motor 202 is fixed to one end of the support 201. A lead screw 203 is installed at the output end of the drive motor 202. The lead screw 203 passes through the support 201 and is rotatably connected to its other wall. A guide rod 204 is fixed parallel to the side of the lead screw 203 on the support 201. A slider 205 is slidably installed on the guide rod 204. A gripper 206 with internal threads is integrally formed on the slider 205. The inner wall of the gripper 206 meshes with the lead screw 203.

[0036] The support 201 is injection molded from ABS material, possessing sufficient structural strength to support the drive components. A drive motor 202 is fixed to one end of the support 201, and a lead screw 203 is installed at the output end of the drive motor 202. The surface of the lead screw 203 is machined with trapezoidal threads, which can convert the rotational motion of the drive motor 202 into linear motion. The lead screw 203 passes through the support 201 and is rotatably connected to its other wall. A miniature bearing is provided at the connection to reduce rotational friction and ensure smooth movement. A slider 205 is slidably mounted on the guide rod 204. The guide rod 204 can limit the slider 205, allowing it to slide back and forth along the guide rod 204. A gripper 206 with internal threads is integrally formed on the slider 205. The inner wall of the gripper 206 meshes with the lead screw 203. When the lead screw 203 rotates, the gripper 206 drives the slider 205 to move linearly along the guide rod 204, realizing the reciprocating scanning of the detection mechanism 300.

[0037] Specifically, the testing mechanism 300 includes an optical path fixing seat 301 mounted on the upper surface of the slider 205. The surface of the optical path fixing seat 301 is provided with three independent limiting grooves, in which a lamp board 302, a filter 303, and an amplification circuit board 304 are respectively fitted and installed. An optical path cover plate 305 is mounted on the upper surface of the optical path fixing seat 301 by bolts.

[0038] The optical path mounting bracket 301 is made of PC material. Its surface has three independent limiting grooves, the depth of which matches the thickness of the corresponding component, ensuring consistent height after installation. The three independent limiting grooves respectively accommodate and install a lamp board 302, a filter 303, and an amplification circuit board 304. The lamp board 302 is a UV LED lamp board, providing stable excitation light for the fluorescent markers on the reagent card. Its VCC and GND pins are soldered to the power output terminal on the main board via enameled wire. The filter 303 has a center wavelength of 365nm, filtering ambient light and stray light to improve the purity of the excitation light. The amplification circuit board... The 304 integrated photoelectric conversion element can convert fluorescent signals into weak electrical signals. Its signal output pin (VOUT) is connected to the input pin of the signal conditioning module 404 in the integrated control system 400 via an FPC flexible cable. The upper surface of the optical path fixing base 301 is bolted with an optical path cover plate 305. The optical path cover plate 305 is made of PC material. By applying pressure evenly, the lamp board 302, filter 303, and amplifier circuit board 304 are pressed and fixed to prevent the components from loosening during movement. The optical path fixing base 301 is quickly plugged in and installed on the side facing the slider 205 through a plug-in structure, which only requires quick installation and removal.

[0039] Specifically, the integrated control system 400 includes a processor module 401, a power supply module 402, a device driver module 403, and a signal conditioning module 404. The processor module 401 is electrically connected to the power supply module 402, the device driver module 403, and the signal conditioning module 404. The device driver module 403 includes a motor driver chip 405, a Bluetooth module 406, and a QR code scanning interface 407. The signal conditioning module 404 includes a second-order RC filter circuit and an instrumentation amplifier.

[0040] The processor module 401 is electrically connected to the power supply module 402, the device driver module 403, and the signal conditioning module 404, and is responsible for coordinating the operation of each module. The processor module 401 and the power supply module 402 are connected via copper foil traces on the motherboard. The output of the power supply module 402 supplies power to the processor module 401, the device driver module 403, the signal conditioning module 404, and the lamp board 302. The processor module 401 and the device driver module 403 are connected using a combination of "SPI bus + GPIO": the SPI_MOSI and SPI_M of the processor module 401... The ISO and SPI_SCK pins are connected to the corresponding pins of the Bluetooth module 406 to achieve high-speed transmission of detection data and reagent information. One GPIO pin of the processor is connected to the reset pin of the Bluetooth module 406 for restarting the Bluetooth module in case of an error. The processor module 401 is connected to the SDA and SCL pins of the QR code scanning interface 407 via the I2C bus to control the startup of the OV7670 image sensor and the reading of QR code data. The power supply of the QR code scanning interface 407 is provided by the power supply module 402, and its signal output is shaped by a Schmitt trigger. The interrupt pin is connected to the processor to enable a fast response after QR code scanning is completed. The enable pin (EN) of the motor driver chip 405 is connected to the GPIO of the processor module 401. The processor starts the motor by pulling the pin low and stops the motor by pulling it high, thus controlling the start and stop of the motor. The power supply of the signal conditioning module 404 is provided by the power supply module 402. Its ground terminal is connected to the common ground of the motherboard through a star grounding method to avoid ground noise interference with signal processing. The output pin of the signal conditioning module 404 is directly connected to the 12-bit ADC input pin of the processor module 401. This enables real-time acquisition of the conditioned electrical signal. Furthermore, one end of the power button is connected to the GPIO input pin of the processor module 401, and the other end is connected to the power supply. When the button is pressed, the pin level is high. The processor determines the power-on command by detecting the duration of the high pin level, triggering the device initialization process. The device driver module 403 includes a motor driver chip 405, a Bluetooth module 406, and a QR code scanning interface 407. The motor driver chip 405 is an A4988 model, which can precisely control the speed and direction of the drive motor 202. The Bluetooth module 406 supports BLE5.The 0 protocol enables stable data transmission with a dedicated APP; the QR code scanning interface 407 is compatible with the OV7670 image sensor, allowing for rapid reading of QR code information (including batch number, expiration date, etc.) on reagent cards; the signal conditioning module 404 includes a second-order RC filter circuit and an instrumentation amplifier. The second-order RC filter circuit has a cutoff frequency of 1kHz, effectively filtering out high-frequency noise. The instrumentation amplifier has adjustable gain, amplifying weak electrical signals to a manageable range and improving the signal-to-noise ratio. The power supply pins (VCC, GND) and control pins (STEP, DIR) of the drive motor 202 are connected to the OUT1, OUT2, STEP, and DIR pins of the motor drive chip 405 in the device drive module 403 via 28AWG shielded wires. The shielding layer is grounded to reduce the impact of electromagnetic interference during motor operation on the circuit signals.

[0041] Specifically, the amplifier circuit board 304 integrates the AD8605 low-noise operational amplifier.

[0042] The amplifier on the amplifier circuit board 304 can effectively suppress dark current interference, ensure accurate conversion of weak fluorescence signals, and provide high-quality raw data for subsequent signal processing.

[0043] Specifically, the processor module 401 is an STM32L476RGT6, and it achieves data transmission without CPU intervention through the built-in DMA controller, thereby improving signal acquisition efficiency. The chip supports a low-power mode, and the processor automatically triggers a shutdown mode after the detection is completed, retaining only the wake-up function of the Bluetooth module 406, which uses the BLE 5.0 protocol.

[0044] The processor module 401 is an STM32L476RGT6, which uses an ARM Cortex-M4 core and has a built-in 12-bit ADC. It can quickly acquire conditioned electrical signals and perform calculations, while also supporting a low-power mode to extend the device's battery life. The Bluetooth module 406 uses the BLE 5.0 protocol and supports AES-128 data encryption, which can securely transmit detection data and ensure the stability of data interaction.

[0045] Specifically, the power module 402 includes a TP4056 charging management chip and an RT9193LDO. In addition, the power module 402 also has a voltage detection circuit (composed of voltage divider resistors and operational amplifiers). Its output terminal is connected to the ADC pin of the processor module 401. The processor calculates the remaining power of the lithium battery by collecting the voltage value after voltage division. When the power is ≤20%, it controls the red light to flash to remind charging.

[0046] The power module 402 includes a TP4056 charging management chip and an RT9193LDO. The TP4056 chip can realize constant current and constant voltage charging of lithium batteries and supports charging protection. The RT9193LDO provides stable power supply for each circuit module, ensuring the stability of signal processing and motor control. At the same time, the power module 402 supports low power management and automatically enters standby mode after detection to reduce energy consumption.

[0047] Working principle: The instrument's measurement system automatically excites the binding region of the fluorescent marker and the analyte on the reaction test card with a laser. After being excited, the fluorescent marker emits fluorescence, and the optical signal is obtained by the photocell on the amplification circuit board 304. Subsequently, the integrated control system 400 filters, amplifies and analyzes the photoelectric signal, providing rapid detection results for multiple samples in a single step.

[0048] Workflow: The reagent card is inserted into the machine through slot 103. The processor module 401 of the integrated control system 400 controls the motor drive chip 405 of the device drive module 403, causing the drive motor 202 to drive the lead screw 203 to rotate. The lead screw 203 drives the detection mechanism 300 to move back and forth along the guide rod 204 in the X direction through the gripper 206. The detection mechanism 300 is fixed on the slider 205, and the guide rod 204 provides motion constraint guidance to ensure the straightness of the scanning trajectory. The detection mechanism 300 is equipped with a sensor head structure, which is precisely positioned by an optocoupler on the main board. During detection, the lamp plate 302 of the detection mechanism 300 emits ultraviolet light, which is filtered by the filter 303 and then irradiates the target area. The reagent card detection window receives ultraviolet light, and the fluorescent marker in the window reflects fluorescence to the photoelectric components of the amplification circuit board 304. The photoelectric components read the spectral information and feed it back to the amplification circuit. The amplification circuit board 304 converts the photoelectric signal into data and transmits it to the integrated control system 400. Users can obtain the analysis results through the indicator light color and flashing status. A flashing blue light indicates that the device is on, and a solid blue light indicates that it is connected via Bluetooth. A solid red light indicates that the device is charging, and a solid red light indicates a positive result when the device is not charging, while a flashing red light indicates that the battery is low. A solid green light indicates that the device is fully charged, and a solid green light indicates a negative result when the device is not charging. Detailed analysis results can also be viewed directly through the APP.

[0049] The specific operating steps are as follows: First, press and hold the power button to turn on the instrument, open the dedicated APP and connect the device via Bluetooth; operate the reagent scanning icon on the APP's "homepage" to scan the reagent QR code information or select the saved reagent information; take the reagent card, drop the sample to be tested into the detection port of the reagent card, wait for the reaction to complete, and insert the test reagent card into slot 103 of the instrument; wait for the instrument to automatically complete the scanning and analysis and obtain the test results. After the measurement is completed, the APP will display and save the current test subject information, test time, results and other data, which can be queried and exported later.

[0050] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0051] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A structure for miniature fluorescence acquisition, analysis, and detection, characterized in that: The device includes a main unit housing (100), inside which a motion mechanism (200) is installed, on which a detection mechanism (300) is installed, on which a motherboard is mounted, and on which an integrated control system (400) is mounted.

2. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 1, characterized in that: The main unit housing (100) includes an upper shell (101) and a lower shell (102). The upper shell (101) and the lower shell (102) are connected by a pin. The bottom of the lower shell (102) is provided with a slot (103). The other end of the lower shell (102) located in the slot (103) is provided with a charging interface (104). The lower shell (102) and the upper shell (101) are provided with a positioning sleeve (105). The positioning sleeve (105) is installed on the inner wall of the lower shell (102) by bolts.

3. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 2, characterized in that: The motion mechanism (200) includes a support (201) fixed in the lower shell (102). A drive motor (202) is fixed to one end of the support (201). A lead screw (203) is installed at the output end of the drive motor (202). The lead screw (203) passes through the support (201) and is rotatably connected to its other wall. A guide rod (204) is fixed parallel to the side of the lead screw (203) on the support (201). A slider (205) is slidably installed on the guide rod (204). A gripper (206) with internal threads is integrally formed on the slider (205). The inner wall of the gripper (206) meshes with the lead screw (203).

4. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 3, characterized in that: The detection mechanism (300) includes an optical path fixing seat (301) mounted on the upper surface of the slider (205). The surface of the optical path fixing seat (301) is provided with three independent limiting grooves. A lamp board (302), a filter (303), and an amplification circuit board (304) are respectively adapted to be installed in the three independent limiting grooves. An optical path cover plate (305) is installed on the upper surface of the optical path fixing seat (301) by bolts.

5. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 1, characterized in that: The integrated control system (400) includes a processor module (401), a power supply module (402), a device driver module (403), and a signal conditioning module (404). The processor module (401) is electrically connected to the power supply module (402), the device driver module (403), and the signal conditioning module (404). The device driver module (403) includes a motor driver chip (405), a Bluetooth module (406), and a QR code scanning interface (407). The signal conditioning module (404) includes a second-order RC filter circuit and an instrumentation amplifier.

6. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 4, characterized in that: The amplifier circuit board (304) integrates an AD8605 low-noise operational amplifier.

7. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 5, characterized in that: The processor module (401) is an STM32L476RGT6, and the Bluetooth module (406) uses the BLE 5.0 protocol.

8. The structure for miniature fluorescence acquisition, analysis, and detection according to claim 5, characterized in that: The power module (402) includes a TP4056 charging management chip and an RT9193LDO.

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