A cryobox for cardiomyocytes

Abstract

This invention discloses a cryopreservation box for cardiomyocytes, belonging to the field of bioengineering technology. It includes a cryopreservation box body, a sealed lid, four fixing plugs, and pressure-applying screws. The four pressure-applying screws slide inside a worm gear and inside two rotating perforated plates. This invention, by setting up independent storage and retrieval structures, allows for the storage of cryopreservation tubes without opening the cryopreservation box body; the tubes can be directly inserted through the perforations, avoiding interference with the preservation of other cryopreservation tubes. When retrieving cells, target cryopreservation tubes can be pushed out in batches and directionally, while cryopreservation tubes not requiring retrieval remain inside the box and are not exposed. This structural design completely avoids disrupting the constant temperature environment inside the box by opening the lid, ensuring that cryopreservation tubes not involved in operation remain in a stable constant temperature state. This prevents metabolic abnormalities or loss of cell activity due to temperature fluctuations, providing a guarantee for the long-term stable cryopreservation of cardiomyocytes.

Description

Technical Field

[0001] This invention belongs to the field of bioengineering technology, specifically relating to a cryopreservation box for cardiomyocytes. Background Technology

[0002] Cardiac cell cryopreservation boxes are low-temperature storage devices specifically designed for cardiomyocytes. They can inhibit the metabolic activity of cardiomyocytes and delay cell aging and apoptosis through a low-temperature environment, thus achieving long-term preservation. At the same time, they ensure that the cells retain normal physiological activity and function after revival.

[0003] When using or storing cryovials in existing cardiomyocyte cryopreservation boxes, the lid usually needs to be opened, which exposes cryovials that do not need to be used or stored inside the box. This disrupts the constant temperature environment inside the box and the cryovials, damaging the constant temperature preservation state of the cells in these cryovials and hindering cell cryopreservation. Therefore, a cryopreservation box for cardiomyocytes is needed to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a cryopreservation box for cardiomyocytes to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cryopreservation box for cardiomyocytes, comprising a cryopreservation box body, a sealed box lid, four fixing plugs and pressure-applying screws, wherein the four pressure-applying screws slide inside a worm gear, slide inside two rotating orifice plates, slide inside four fixing collars, and each of the four pressure-applying screws has a rotating handle fixedly connected to its top. A sealing strip is inserted and connected inside the cryopreservation box body, a cryopreservation chamber is fixedly connected inside the cryopreservation box body, a placement rack is fixedly connected inside the cryopreservation chamber, eight limiting bottom rings are rotatably connected inside the placement rack, springs are fixedly connected to the tops of the eight limiting bottom rings, storage racks are fixedly connected to the tops of the eight springs, and cell cryopreservation tubes are inserted and connected inside the eight storage racks.

[0006] By setting up independent storage and retrieval structures, cell cryovials can be stored without opening the cryopreservation box itself, and can be directly inserted through the openings, avoiding the impact of opening the box on the preservation of other cryovials. When retrieving, the target cryovials can be pushed out in batches and in a directional manner, while cryovials that do not need to be retrieved remain inside the box and are not exposed. Under the above structural design, the constant temperature environment inside the box can be completely avoided by opening the box lid, ensuring that cryovials not involved in the operation are in a stable constant temperature state, preventing metabolic abnormalities or loss of activity of cells in the tubes due to temperature fluctuations, and providing a guarantee for the long-term stable cryopreservation of cardiomyocytes.

[0007] As a preferred embodiment, the bottom of the sealing lid fits into the top of the cryopreservation box body.

[0008] As a preferred embodiment, the four fixing bolts are respectively inserted and connected to the sealing box cover, and the four fixing bolts are respectively inserted and connected to the body of the cryopreservation box.

[0009] As a preferred embodiment, the top of the sealing box cover is fixedly connected with eight first marks, and the interior of the sealing box cover has a limiting groove.

[0010] As a preferred embodiment, a worm gear is rotatably connected inside the sealing cover, and a worm wheel is meshed with the surface of the worm gear, the worm wheel rotating inside the limiting groove.

[0011] As a preferred embodiment, the two ends of the worm gear are respectively fixedly connected to rotating disks, and the two rotating disks rotate inside the sealed box cover.

[0012] As a preferred embodiment, four fixing collars are fixedly connected to the top of one of the rotating discs, and two first sliding grooves are respectively formed on the top of the four fixing collars.

[0013] As a preferred embodiment, the interior of each of the four fixing collars is provided with a second sliding groove, the first sliding groove and the second sliding groove are connected, and the surface of each of the four fixing collars is fixedly connected with a second mark.

[0014] By incorporating a spring, the cryovials can be smoothly stored when squeezed. When needed, the spring automatically and slowly pushes the storage rack out, facilitating retrieval and effectively improving operational convenience. In addition, the designed first marker can be used with a worm gear and worm wheel to drive a rotating disc for adjustment, which can quickly locate the target cell cryovial and align the fixing ring with the storage rack, reducing operation time and improving storage and retrieval efficiency. The combination structure of the pressure-applying rotating column with the slider, the first slide groove, and the second slide groove can quickly and flexibly fix the cell cryovials, balancing operational convenience and storage safety.

[0015] Compared with the prior art, the beneficial effects of this utility model are: This invention, through its independent storage and retrieval structure, allows for the storage of cell cryovials without opening the cryopreservation box itself; the tubes can be directly inserted through the openings, avoiding disruption to the preservation of other cryovials. When retrieving cells, target cryovials can be pushed out in batches and in a directional manner, while cryovials not requiring retrieval remain inside the box and are not exposed. This structural design completely prevents the opening of the box from disrupting the constant temperature environment, ensuring that cryovials not being used remain at a stable temperature. This prevents metabolic abnormalities or loss of cell activity due to temperature fluctuations, providing a guarantee for the long-term stable cryopreservation of cardiomyocytes.

[0016] This invention features a spring mechanism. Squeezing the spring allows for easy storage of cryopreservation tubes, while the spring automatically and slowly pushes the storage rack out for convenient retrieval, significantly improving operational convenience. Furthermore, the designed first marker, combined with a worm gear and worm wheel to drive a rotating disc, enables rapid positioning of the target cell cryopreservation tube and alignment of the fixing ring with the storage rack, reducing operation time and improving storage efficiency. The combined structure of the pressure-applying rotating column, slider, first groove, and second groove allows for quick and flexible fixation of the cell cryopreservation tubes, balancing operational convenience with storage safety. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a schematic diagram of the internal cross-section of the present invention; Figure 3 This is a schematic diagram of the structure of the present invention in an explosion. Figure 4 This is a schematic diagram of the pressure-applying rotating column of this utility model.

[0018] In the diagram: 1. Cryopreservation box body; 2. Sealing box lid; 3. Fixing bolt; 4. First mark; 5. Limiting groove; 6. Worm gear; 7. Worm wheel; 8. Rotating plate; 9. Fixing collar; 10. First slide groove; 11. Second slide groove; 12. Second mark; 13. Pressure-applying rotating column; 14. Rotating handle; 15. Sealing strip; 16. Cryopreservation chamber; 17. Placement rack; 18. Limiting bottom ring; 19. Spring; 20. Storage rack; 21. Cell cryopreservation tube. Detailed Implementation

[0019] The present invention will be further described below with reference to the embodiments.

[0020] The following embodiments are used to illustrate the present invention, but should not be used to limit the scope of protection of the present invention. The conditions in the embodiments can be further adjusted according to specific conditions, and simple improvements to the method of the present invention under the premise of the concept of the present invention are all within the scope of protection claimed by the present invention.

[0021] Please see Figure 1-4This utility model provides a cryopreservation box for cardiomyocytes, including a cryopreservation box body 1, a sealing box cover 2, four fixing plugs 3, and pressure-applying rotating columns 13. The four pressure-applying rotating columns 13 slide inside a worm gear 7, inside two rotating orifice plates 8, and inside four fixing collars 9. A rotating handle 14 is fixedly connected to the top of each of the four pressure-applying rotating columns 13. A sealing strip 15 is inserted and connected inside the cryopreservation box body 1. A cryopreservation chamber 16 is fixedly connected inside the cryopreservation box body 1. A placement rack 17 is fixedly connected inside the cryopreservation chamber 16. Eight limiting bottom rings 18 are rotatably connected inside the placement rack 17. The tops of the eight limiting bottom rings 18 are fixedly connected to the bottom of each of the four fixed bottom rings 9. The system is fixedly connected to eight springs 19, with storage racks 20 fixedly connected to the top of each spring 19. Cell cryopreservation tubes 21 are inserted into the interior of each of the eight storage racks 20. By setting up an independent storage and retrieval structure, cell cryopreservation tubes 21 can be directly inserted through the openings without opening the cryopreservation box body 1 when storing them, avoiding the impact of opening the box on the preservation of other cryopreservation tubes. When retrieving them, the target cryopreservation tubes can be pushed out in batches and in a directional manner, while cryopreservation tubes that do not need to be retrieved remain in the box and are not exposed. Under the above structural design, the constant temperature environment inside the box can be completely avoided by opening the box lid, ensuring that cryopreservation tubes not involved in the operation are in a stable constant temperature state, preventing metabolic abnormalities or loss of activity of cells in the tubes due to temperature fluctuations, and providing a guarantee for the long-term stable cryopreservation of cardiomyocytes.

[0022] The bottom of the sealed box lid 2 fits against the top of the cryopreservation box body 1.

[0023] Four fixing bolts 3 are respectively inserted and connected to the sealing box cover 2, and four fixing bolts 3 are respectively inserted and connected to the body of the cryopreservation box 1.

[0024] The top of the sealing box cover 2 is fixedly connected with eight first marks 4, and the inside of the sealing box cover 2 is provided with a limiting groove 5.

[0025] The inside of the sealed box cover 2 is rotatably connected to a worm gear 6, and the surface of the worm gear 6 is meshed with a worm wheel 7, which rotates inside the limiting groove 5.

[0026] Two rotating disks 8 are fixedly connected to both ends of the worm gear 7, and the two rotating disks 8 rotate inside the sealed box cover 2.

[0027] Four fixing collars 9 are fixedly connected to the top of a rotating hole plate 8, and two first sliding grooves 10 are respectively opened on the top of the four fixing collars 9.

[0028] The four fixing collars 9 are each provided with a second groove 11 inside. The first groove 10 and the second groove 11 are connected. The surfaces of the four fixing collars 9 are respectively fixedly connected with second marks 12. By setting springs 19, the cryopreservation tubes can be smoothly stored by squeezing the springs 19. When needed, the springs 19 automatically and slowly push the storage rack 20 to pop out, making it easy to retrieve and effectively improving the convenience of operation. In addition, the designed first mark 4 can be used with worm gear 6 and worm wheel 7 to drive the rotating hole plate 8 for adjustment, which can quickly position the target cell cryopreservation tube 21 and align the fixing collar 9 and the storage rack 20, reducing operation time and improving storage and retrieval efficiency. The cooperation structure of the pressure-applying rotating column 13 with the slider, the first groove 10 and the second groove 11 can quickly and flexibly fix the cell cryopreservation tube 21, taking into account both the convenience of operation and the safety of storage.

[0029] Working principle and usage process of this utility model: In operation, the present invention first rotates within the fixing collar 9 and pulls out the pressure-applying column 13, inserting the cell cryopreservation tube 21 containing myocardial cells into the corresponding storage rack 20 through the gaps of the fixing collar 9, the rotating plate 8, and the worm gear 7; then the pressure-applying column 13 is inserted, its bottom contacting the cell cryopreservation tube 21 and squeezing the spring 19, so that the cell cryopreservation tube 21 slides completely into the cryopreservation box body 1 for constant temperature preservation; then the pressure-applying column 13 is rotated, allowing its surface slider to be inserted into the second slide groove 11 through the first slide groove 10 for fixation, thus completing the storage of the cell cryopreservation tube 21; When retrieving cryovials, first locate the target cell cryovial 21 according to the first mark 4 on the top of the sealed box cover 2. Rotate the worm gear 6 to drive the worm wheel 7 and the rotating plate 8 so that the corresponding fixing collar 9 is aligned with the target storage rack 20. Then rotate the pressure column 13 to let the slider slide from the second slide groove 11 to the first slide groove 10. Slowly pull out the pressure column 13. The squeezed spring 19 gradually rebounds and slowly pushes the storage rack 20 and the cell cryovial 21 out of the cell cryopreservation chamber 16 through the worm wheel 7, the rotating plate 8 and the fixing collar 9. At this time, the cell cryopreservation chamber 16 can be retrieved, while the cell cryovial 21 that does not need to be retrieved remains in the box for constant temperature preservation. After use, the cell cryopreservation tube 21 is returned to its original position and fixed in the same way. The process does not require opening the cryopreservation box body 1, which can reduce the impact on other cell cryopreservation tubes 21 in the box.

[0030] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cryopreservation box for cardiomyocytes, comprising a cryopreservation box body (1), a sealing box lid (2), four fixing plugs (3), and a pressure-applying rotating column (13), characterized in that: The four pressure-applying rotary columns (13) slide inside the worm gear (7), the four pressure-applying rotary columns (13) slide inside the two rotating discs (8), the four pressure-applying rotary columns (13) slide inside the four fixed collars (9), and the tops of the four pressure-applying rotary columns (13) are respectively fixedly connected to rotating handles (14). The inside of the cryopreservation box body (1) is inserted and connected to a sealing strip (15). The inside of the cryopreservation box body (1) is fixedly connected to a cryopreservation chamber (16). The inside of the cryopreservation chamber (16) is fixedly connected to a placement rack (17). The inside of the placement rack (17) is rotatably connected to eight limiting bottom rings (18). The tops of the eight limiting bottom rings (18) are respectively fixedly connected to springs (19). The tops of the eight springs (19) are respectively fixedly connected to storage racks (20). The insides of the eight storage racks (20) are respectively inserted and connected to cell cryopreservation tubes (21).

2. The cryopreservation box for cardiomyocytes according to claim 1, characterized in that: The bottom of the sealed box cover (2) is attached to the top of the cryopreservation box body (1).

3. The cryopreservation box for cardiomyocytes according to claim 1, characterized in that: The four fixing bolts (3) are respectively inserted and connected to the sealing box cover (2), and the four fixing bolts (3) are respectively inserted and connected to the body of the cryopreservation box (1).

4. A cryopreservation box for cardiomyocytes according to claim 3, characterized in that: The top of the sealing box cover (2) is fixedly connected with eight first marks (4), and the inside of the sealing box cover (2) is provided with a limiting groove (5).

5. A cryopreservation box for cardiomyocytes according to claim 4, characterized in that: The sealing box cover (2) is rotatably connected to a worm gear (6), and a worm wheel (7) is meshed with the surface of the worm gear (6). The worm wheel (7) rotates inside the limiting groove (5).

6. A cryopreservation box for cardiomyocytes according to claim 5, characterized in that: The two ends of the worm gear (7) are respectively fixedly connected to rotating discs (8), and the two rotating discs (8) rotate inside the sealed box cover (2).

7. A cryopreservation box for cardiomyocytes according to claim 6, characterized in that: Four fixing collars (9) are fixedly connected to the top of one of the rotating hole discs (8), and two first sliding grooves (10) are respectively opened on the top of the four fixing collars (9).

8. A cryopreservation box for cardiomyocytes according to claim 7, characterized in that: The four fixed collars (9) are respectively provided with a second groove (11), the first groove (10) and the second groove (11) are connected, and the surface of the four fixed collars (9) is respectively fixedly connected with a second mark (12).

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