Inward-rotating biological cryopreservation tube

Abstract

The utility model discloses an inner rotation formula biological freeze storage tube, including the freeze storage tube main part, the top fixedly connected with screw joint of freeze storage tube main part, the upper portion of freeze storage tube main part is provided with the sealed pipe cover, the bottom surface fixedly connected with the pipe cover screwing post of screw joint adaptation of sealed pipe cover, the outer surface of pipe cover screwing post is provided with screwing post outer thread, the inner wall of screw joint is provided with the interface inner rotation thread of screwing post outer thread adaptation. This inner rotation formula biological freeze storage tube adopts the inner rotation cooperation of pipe cover screwing post and screw joint, and screwing post outer thread and interface inner rotation thread are built -in, avoid the thread exposure and be polluted by sample, and when screwing, the main sealing ring is closely attached with the limiting step, forms the first sealed defense line, and at the same time, the pipe cover screwing post is inserted into freeze storage tube main part, and the sealing path is shortened, and the sealing accuracy is improved, realizes the inner rotation formula sealing structure, and the sealing precision is accurate and prevents pollution.

Description

Technical Field

[0001] This utility model relates to the field of biological sample storage technology, and in particular to an internally rotating biological cryopreservation tube. Background Technology

[0002] Cryopreservation tubes are mainly used for the low-temperature transport and storage of tissue or cell samples. They are commonly used in biological research and medical fields. Generally, cryopreservation tubes are made of non-cytotoxic materials. Commonly used materials in laboratories include plastic and glass. However, because glass cryopreservation tubes cannot be used in high-speed or ultracentrifuges, plastic cryopreservation tubes are more commonly used. Cryopreservation tubes are suitable for the preservation and low-temperature refrigeration of serum, culture medium, cells, tissues, and other reagents.

[0003] Cryopreservation tubes are the core carriers for storing biological samples. Their sealing reliability, freeze-thaw resistance, crack resistance, and sample traceability directly determine the quality of sample storage and the safety of use. Traditional cryopreservation tubes have some shortcomings in practical applications. The threads of externally spiral-sealed tubes are exposed and easily contaminated by samples or accumulate impurities, leading to poor screwing. On the other hand, plug-in seals rely on a single sealing ring, which is prone to hardening and shrinking during cryopreservation, resulting in sealing gaps, sample leakage, or intrusion of external moisture, affecting sample viability. To address these issues, we propose an internally spiral-sealed cryopreservation tube. Utility Model Content

[0004] The purpose of this invention is to provide an internally rotating cryopreservation tube to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: An internally spiral-type cryopreservation tube includes a cryopreservation tube body, a screw-on interface fixedly connected to the top of the cryopreservation tube body, a sealing cap disposed above the cryopreservation tube body, a cap screw-on post adapted to the screw-on interface fixedly connected to the bottom surface of the sealing cap, an external thread of the cap screw-on post disposed on the outer surface of the cap screw-on post, an internal thread of the interface adapted to the external thread of the cap screw-on interface disposed on the inner wall of the cap screw-on interface, a limiting step located above the internal thread of the interface fixedly connected to the inner wall of the cap screw-on post, a main sealing ring corresponding to the limiting step fixedly connected to the upper part of the outer surface of the cap screw-on post, and a sealing reinforcement component disposed between the sealing cap and the screw-on interface.

[0006] In a further embodiment, an inner sealing gasket is fixedly connected to the inner wall of the cryopreservation tube body. The inner sealing gasket is located below the internal thread of the interface, and the inner diameter of the inner sealing gasket is adapted to the outer diameter of the tube cap screw.

[0007] In a further embodiment, the sealing enhancement component includes a mounting groove formed on the screw-on interface and a bottom sealing ring fixedly connected in the mounting groove. A top sealing ring adapted to the bottom sealing ring is fixedly connected to the bottom surface of the sealing tube cover, and the top sealing ring and the main sealing ring are located on the same horizontal line.

[0008] In a further embodiment, an identification area is provided on the outer surface of the cryopreservation tube body.

[0009] In a further embodiment, the outer surface of the sealing tube cap is provided with annularly arranged anti-slip grooves, and the bottom end of the cryopreservation tube body is fixedly connected to an anti-slip base.

[0010] In a further embodiment, the outer diameter of the cap screw-on post is adapted to the inner diameter of the cryopreservation tube body and the screw-on interface, and the cap screw-on post can be inserted into the screw-on interface and the cryopreservation tube body.

[0011] Compared with the prior art, the beneficial effects of this utility model are: This device employs an internal screw-on fit between the cap screw-on post and the screw-on interface. The external thread of the screw-on post and the internal thread of the interface are internally integrated to prevent the exposed threads from being contaminated by the sample. During screwing, the main sealing ring and the limiting step fit tightly together, forming the first line of defense for sealing. At the same time, the cap screw-on post is inserted into the cryopreservation tube body, shortening the sealing path and improving sealing accuracy, achieving an internal screw-on sealing structure that provides precise sealing and prevents contamination. Furthermore, based on the main sealing ring, the top and bottom sealing rings of the sealing reinforcement component form a second annular seal, and the inner sealing gasket fits against the bottom of the cap screw-on post to form a third end face seal. This triple sealing structure ensures that the seal remains intact even under harsh conditions such as low temperature, vibration, and repeated freeze-thaw cycles, completely eliminating sample leakage and contamination. Attached Figure Description

[0012] Figure 1 A schematic diagram of the overall three-dimensional structure of an internally spiral-type cryopreservation tube; Figure 2 This is a top view of the main body of an internally spiral-type cryopreservation tube. Figure 3 A bottom view of the sealing cap and cap screwing post in an internally spiral-type cryopreservation tube; Figure 4 This is a schematic diagram showing the separation of the cryopreservation tube body and the sealing cap in an internally spiral-type cryopreservation tube.

[0013] In the diagram: 1. Cryopreservation tube body; 2. Identification area; 3. Anti-slip base; 4. Screw-in interface; 5. Sealing cap; 6. Sealing reinforcement component; 61. Mounting groove; 62. Bottom sealing ring; 63. Top sealing ring; 7. Inner sealing gasket; 8. Internal thread of the interface; 9. Limiting step; 10. Main sealing ring; 11. Cap screw-in post; 12. External thread of the screw-in post; 13. Anti-slip groove. Detailed Implementation

[0014] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0015] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Please see Figure 1-4In this utility model, an internally spiral-type cryopreservation tube includes a cryopreservation tube body 1. A screw-on interface 4 is fixedly connected to the top of the cryopreservation tube body 1. A sealing cap 5 is disposed above the cryopreservation tube body 1. A cap screw-on post 11, adapted to the screw-on interface 4, is fixedly connected to the bottom surface of the sealing cap 5. An external thread 12 is provided on the outer surface of the cap screw-on post 11. An internal spiral thread 8, adapted to the external thread 12, is provided on the inner wall of the screw-on interface 4. A [unclear - possibly a component or part] is fixedly connected to the inner wall of the screw-on interface 4. The upper part of the outer surface of the tube cap engagement post 11 is fixedly connected to the limiting step 9 above the internal thread 8, and the main sealing ring 10 corresponding to the limiting step 9 is fixedly connected to the upper part of the tube cap engagement post 11. A sealing enhancement component 6 is also provided between the sealing tube cap 5 and the engagement interface 4. The internal screw-fit between the tube cap engagement post 11 and the engagement interface 4 avoids thread contamination. The main sealing ring 10 and the limiting step 9 form a basic seal. The sealing enhancement component 6 further improves the sealing performance, solves the core problems of poor sealing and thread contamination in traditional cryogenic tubes, and takes into account both sealing reliability and anti-contamination.

[0018] An inner sealing gasket 7 is fixedly connected to the inner wall of the cryopreservation tube body 1. The inner sealing gasket 7 is located below the inner thread 8 of the interface, and the inner diameter of the inner sealing gasket 7 is adapted to the outer diameter of the tube cap screw-in post 11. The inner sealing gasket 7 is located below the inner thread 8 of the interface and fits tightly with the bottom of the inserted tube cap screw-in post 11, forming a third end face seal, which fills the defect of the traditional single radial seal. The inner sealing gasket 7 is made of low temperature resistant elastic material, which can maintain elasticity even in the -196℃ liquid nitrogen cryopreservation environment, avoiding the formation of sealing gaps. The sealing reinforcement component 6 includes an installation part opened on the screw-in interface 4. The bottom sealing ring 62 is fixedly connected to the mounting groove 61. The bottom surface of the sealing tube cover 5 is fixedly connected to the bottom sealing ring 62 and the top sealing ring 63 is adapted to the bottom sealing ring 62. The top sealing ring 63 and the main sealing ring 10 are located on the same horizontal line. The mounting groove 61 of the sealing enhancement component 6 fixes the bottom sealing ring 62. The top sealing ring 63 of the sealing tube cover 5 and the bottom sealing ring 62 fit precisely to form an annular radial seal. This forms a double radial seal layout with the radial seal of the main sealing ring 10. The two sealing rings are located on the same horizontal line and can withstand pressure at the same time, improving the uniformity and stability of the seal.

[0019] The outer surface of the cryopreservation tube body 1 is provided with a marking area 2, which is a dedicated information observation area. The outer surface of the sealing cap 5 is provided with annularly arranged anti-slip grooves 13. The bottom end of the cryopreservation tube body 1 is fixedly connected to an anti-slip base 3. The annular anti-slip grooves 13 of the sealing cap 5 increase the contact friction between the fingers and the cap, especially when wearing low-temperature gloves or when hands are wet, making it easy to tighten or loosen, avoiding slippage that could lead to incomplete tightening. The anti-slip base 3 at the bottom end of the cryopreservation tube body 1 is made of rubber. To increase friction with the cryopreservation rack and improve placement stability, the outer diameter of the cap screw-on post 11 is adapted to the inner diameter of the cryopreservation tube body 1 and the screw-on interface 4. The cap screw-on post 11 can be inserted into the screw-on interface 4 and the cryopreservation tube body 1. The outer diameter of the cap screw-on post 11 is precisely adapted to the inner diameter of the cryopreservation tube body 1 and the screw-on interface 4. After insertion, a tight gap fit is formed, reducing the air flow between the tube and the outside. At the same time, the extended screw-on post can penetrate deep into the cryopreservation tube body 1, shortening the distance between the sample and the sealing structure and improving the overall sealing integrity.

[0020] The working principle of this utility model is as follows: Check that the cryopreservation tube body 1 is undamaged and free of cracks, and that the sealing cap 5 fits smoothly with the screw-on interface 4. Check that the main sealing ring 10, inner sealing gasket 7, and sealing reinforcement component 6 are free of deformation and aging, ensuring that the sealing structure is intact. After the sample is placed into the cryopreservation tube body 1, align the cap screw-on post 11 of the sealing cap 5 with the screw-on interface 4, insert it into the cryopreservation tube body 1, and rotate it counterclockwise to make the external thread 12 of the screw-on post precisely engage with the internal thread 8 of the interface. Continue rotating until the main sealing ring 10 and the limiting step 9 are tightly fitted, and at the same time the top sealing ring 63 and the bottom sealing ring 62 are fitted. Stop rotating when you feel obvious resistance, and the seal is complete. When you need to open it, hold the anti-slip groove 13 of the sealing cap 5 and rotate it clockwise to unscrew it from the screw-on interface 4 of the cryopreservation tube body 1 to achieve separation and opening.

[0021] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0022] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An internally rotating cryopreservation tube, characterized in that: The system includes a cryopreservation tube body (1), a screw-on interface (4) fixedly connected to the top of the cryopreservation tube body (1), a sealing tube cap (5) provided above the cryopreservation tube body (1), a tube cap screw-on post (11) adapted to the screw-on interface (4) fixedly connected to the bottom surface of the sealing tube cap (5), a screw-on post external thread (12) provided on the outer surface of the tube cap screw-on post (11), an interface internal thread (8) adapted to the screw-on post external thread (12) provided on the inner wall of the screw-on interface (4), a limiting step (9) located above the interface internal thread (8) fixedly connected to the inner wall of the screw-on interface (4), a main sealing ring (10) corresponding to the limiting step (9) fixedly connected to the upper part of the outer surface of the tube cap screw-on post (11), and a sealing reinforcement component (6) provided between the sealing tube cap (5) and the screw-on interface (4).

2. The internally spiral-type cryopreservation tube according to claim 1, characterized in that: The inner wall of the cryopreservation tube body (1) is fixedly connected with an inner sealing gasket (7). The inner sealing gasket (7) is located below the inner thread (8) of the interface, and the inner diameter of the inner sealing gasket (7) is adapted to the outer diameter of the tube cap screw post (11).

3. The internally spiral-type cryopreservation tube according to claim 1, characterized in that: The sealing enhancement component (6) includes a mounting groove (61) opened on the screw-in interface (4) and a bottom sealing ring (62) fixedly connected in the mounting groove (61). The bottom surface of the sealing tube cover (5) is fixedly connected with a top sealing ring (63) that is compatible with the bottom sealing ring (62), and the top sealing ring (63) and the main sealing ring (10) are located on the same horizontal line.

4. The internally spiral-type cryopreservation tube according to claim 1, characterized in that: The outer surface of the cryopreservation tube body (1) is provided with an identification area (2).

5. The internally spiral-type cryopreservation tube according to claim 1, characterized in that: The outer surface of the sealing tube cap (5) is provided with annular anti-slip grooves (13), and the bottom end of the cryopreservation tube body (1) is fixedly connected to an anti-slip base (3).

6. The internally spiral-type cryopreservation tube according to claim 1, characterized in that: The outer diameter of the cap screw-on post (11) is adapted to the inner diameter of the cryopreservation tube body (1) and the screw-on interface (4), and the cap screw-on post (11) can be inserted into the screw-on interface (4) and the cryopreservation tube body (1).

Images