A special device for fixing animals for detecting pain behavior of mouse lower limbs

Abstract

The application relates to the technical field of mouse lower limb pain behavior detection devices, and provides a special animal fixing device suitable for mouse lower limb pain behavior detection, which comprises a restraint belt and a transparent limiting box, the limiting box is internally provided with a containing cavity and is used for containing experimental mice, and a plurality of stimulating holes in communication with the containing cavity are arranged in the bottom of the limiting box; the restraint belt is arranged in the containing cavity and is connected with the limiting box, and the restraint belt is used for restraining the torso of the experimental mice. The restraint belt arranged in the limiting box avoids body movement and posture change of the experimental mice in the process of plantar pain behavior detection, and the experiment can be carried out without static placement, so that the experiment time is greatly reduced; and since the torso of the experimental mice is restrained without fixing the limbs, the reaction of the experimental mice to the pain stimulation is not affected, accurate and highly repeatable experimental results can be obtained, and the accuracy of experimental data is greatly improved.

Description

Technical Field

[0001] This application relates to the technical field of rat hind limb pain behavior detection devices, and more particularly to a special animal fixation device suitable for rat hind limb pain behavior detection. Background Technology

[0002] With the improvement of socioeconomic level and medical technology, people's demand for comfortable medical care has gradually become more widespread, and the study of pain mechanisms and the improvement of diagnosis and treatment methods have gradually received attention from clinical and basic medicine. In order to conduct in-depth research on the pathogenesis of pain and the effects of new analgesic drugs or technologies, it is often necessary to construct pain models in experimental animals and conduct relevant pain behavioral tests.

[0003] Currently, the general method for behavioral testing of plantar pain in this model involves placing mice in an experimental box. To obtain accurate and reliable experimental data, the mice must be kept still, quiet, and not asleep before stimulation; that is, their limbs should be naturally flat against the bottom mesh screen, their heads and bodies naturally extended, without shaking their heads, sniffing, standing, or lying on their sides. Existing experimental boxes have a perforated mesh screen at the bottom. After the mice have adapted to the environment and are quiet, different stimulation methods (cilia mechanosensitive stimulation, acetone cold stimulation, and 22G needle analgesic stimulation) are sequentially applied vertically through the mesh screen to stimulate the hind paw on the surgical side of the rat. Raising the paw, licking the paw, or making a vocalization is recorded as a positive result. The experiment is repeated several times, and the results are recorded.

[0004] In existing technologies, mice are often placed in experimental boxes and left to rest for at least 30 minutes to adapt to the environment and remain calm. However, existing experimental boxes cannot restrict the mice's posture. After stimulation, the mice often become active again, exhibiting postures such as standing, turning, raising their legs, trembling, and lying on their sides. This results in a long waiting time between each stimulation, significantly extending the experimental time. Furthermore, since the mice's responses may differ after each stimulation, it can easily affect the accuracy of the experimental data. Utility Model Content

[0005] In view of the shortcomings of the prior art, the purpose of this application is to provide a special animal restraint device for detecting rat hind limb pain behavior, which aims to solve the problem that existing experimental boxes cannot restrict the experimental rats.

[0006] The technical solution adopted by this application to solve the technical problem is as follows: A special animal fixation device suitable for behavioral detection of lower limb pain in mice, comprising: a transparent limiting box, wherein the limiting box has a receiving cavity for accommodating experimental mice, and the bottom of the limiting box is provided with a plurality of stimulation holes communicating with the receiving cavity.

[0007] A restraint strap is disposed within the receiving cavity and connected to the limiting box; the restraint strap is used to restrain the torso of the experimental mouse.

[0008] Furthermore, the restraint strap includes a restraint portion, which is used to be fitted onto the torso of the experimental mouse to restrain the experimental mouse.

[0009] Two connecting parts, one end of each connecting part is connected to the two opposite ends of the binding part, and the other end is detachably connected to the limiting box.

[0010] Furthermore, the binding portion includes: a first binding segment, the two opposite ends of the first binding segment being respectively connected to the two connecting portions;

[0011] The second restraint segment has two opposite ends that are detachably connected to the two connecting parts. The first restraint segment and the second restraint segment form a ring structure and are used to be fitted onto the torso of the experimental mouse to restrain the experimental mouse.

[0012] Furthermore, the second restraint segment is detachably connected to the two connecting parts via Velcro.

[0013] Furthermore, the limiting box includes: an annular body portion, and the limiting box is provided with a sliding hole;

[0014] The mesh screen portion covers one end of the main body portion and is provided with an opening, and a plurality of the stimulation holes are provided on the mesh screen portion;

[0015] A first movable joint is disposed on the body portion and located outside the receiving cavity;

[0016] One of the connecting portions has one end opposite to the binding portion passing through the sliding hole and is provided with a second swivel portion for detachable connection with the first swivel portion.

[0017] Furthermore, the first swivel part is a hook, and the tip of the hook is disposed away from the sliding hole, and the second swivel part is annular in shape.

[0018] Furthermore, the restraint strap also includes a limiting part, which is disposed at one end of the connecting part passing through the sliding hole away from the restraint part, and the limiting part is used to restrict the connecting part from disengaging from the sliding hole.

[0019] Furthermore, the limiting box also includes a cover plate, which covers the opening at the other end of the main body, and the cover plate is detachably disposed from the main body.

[0020] Furthermore, the cover plate is provided with several ventilation holes.

[0021] Furthermore, the animal restraint device also includes an adjustment buckle, which is disposed on the connecting part and is used to adjust the length of the connecting part.

[0022] Compared with the prior art, this utility model avoids changes in body movement and posture of experimental mice during the foot pain behavior test by setting restraint straps in the limiting box, and the experiment can be conducted without being placed still, which greatly reduces the experimental time. Moreover, since the experimental mice are restrained in their torso but not in their limbs, their response to pain stimuli will not be affected, and accurate and highly repeatable experimental results can be obtained, which greatly improves the accuracy of experimental data. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of the animal fixation device for detecting hind limb pain in rats provided in this embodiment;

[0025] Figure 2 This is a partial cross-sectional view of the animal restraint device for detecting hind limb pain behavior in rats provided in this embodiment;

[0026] Figure 3 yes Figure 2 A magnified view of a portion of point A;

[0027] Figure 4 This is a partially enlarged schematic diagram of an animal restraint device for detecting hind limb pain behavior in rats, provided in another embodiment;

[0028] Figure 5 This is a schematic diagram of the overall structure of the restraint belt provided in this embodiment.

[0029] In the diagram: 100, limiting box; 110, main body; 111, sliding hole; 120, screen part; 121, stimulation hole; 130, cover plate; 131, vent hole; 140, first movable joint; 200, restraint strap; 210, restraint part; 211, first restraint section; 212, second restraint section; 213, Velcro; 220, connecting part; 230, second movable joint; 240, limiting part; 250, adjusting buckle. Detailed Implementation

[0030] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0031] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0032] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0033] Furthermore, the technical features involved in the different embodiments of the present invention described above can be combined with each other as long as they do not conflict with each other.

[0034] This utility model provides, for example Figures 1 to 5 The device shown is an animal restraint device for behavioral testing of lower limb pain in mice, designed to address the shortcomings of existing experimental boxes that cannot confine laboratory mice.

[0035] This animal restraint device, applicable to behavioral testing of lower limb pain in mice, mainly comprises: a restraint strap 200 and a transparent containment box 100. The containment box 100 has a receiving cavity for containing the experimental mouse, and several stimulation holes 121 communicating with the receiving cavity are provided through the bottom of the containment box 100. The restraint strap 200 is placed inside the receiving cavity and connected to the containment box 100, and is used to restrain the torso of the experimental mouse.

[0036] Specifically, the containment box 100 is made of transparent plastic and is rectangular in shape for easy observation by experimenters. Stimulation holes 121 are distributed at the bottom of the containment box 100 and can be used to apply stimulation to the soles of the mice's feet through the stimulation holes 121 during behavioral testing.

[0037] In practical use, after the mouse's torso is restrained by the restraint straps 200, the mouse is placed into the containment chamber. At this time, the mouse is restrained by the restraint straps 200 within the containment box 100, with its limbs resting on the bottom mesh screen. Stimulation can be applied to the soles of the mouse's feet through the stimulation holes 121. Since the mouse's body movement and posture changes are avoided during the foot pain behavior test, the experiment can be conducted without resting, greatly improving experimental efficiency. On the other hand, by restricting the mouse's movement but not fixing its limbs, its response to pain stimuli is not restricted, resulting in accurate and highly repeatable experimental results, greatly improving the accuracy of experimental data.

[0038] In existing technologies, mice are often placed in experimental boxes and left to rest for at least 30 minutes to adapt to the environment and remain calm. However, existing experimental boxes cannot restrict the mice's posture. After stimulation, the mice often become active again, exhibiting postures such as standing, turning, raising their legs, trembling, and lying on their sides. This results in a long waiting time between each stimulation, significantly extending the experimental time. Furthermore, since the mice's responses may differ after each stimulation, it can easily affect the accuracy of the experimental data.

[0039] This invention, by setting a restraint strap 200 inside the limiting box 100, avoids changes in body movement and posture of experimental mice during the foot pain behavior test, allowing the experiment to be conducted without rest, greatly reducing the experimental time; and because the experimental mice's torso is restrained but their limbs are not fixed, their response to pain stimuli is not affected, and accurate and highly repeatable experimental results can be obtained, greatly improving the accuracy of experimental data.

[0040] In some embodiments, such as Figure 2 , Figure 5 As shown, the restraint strap 200 includes a restraint portion 210, which is used to be fitted onto the torso of the experimental mouse to restrain the experimental mouse.

[0041] Two connecting parts 220, one end of each connecting part 220 is connected to the two opposite ends of the restraint part 210, and the other end is detachably connected to the limiting box 100.

[0042] Specifically, the two ends of the two connecting parts 220 are respectively connected to the two ends of the receiving cavity in the horizontal direction. In actual use, by setting two connecting parts 220 of appropriate length, the restraint part 210 is located in the middle of the receiving cavity, and the limbs of the experimental mouse are placed on the bottom mesh screen, which makes it convenient to apply stimulation to the soles of the experimental mouse through the stimulation hole 121.

[0043] In some embodiments, such as Figure 2 , Figure 5 As shown, the binding part 210 includes: a first binding segment 211, the two opposite ends of the first binding segment 211 being connected to two connecting parts 220 respectively;

[0044] The second restraint segment 212 has two opposite ends that are detachably connected to two connecting parts 220. The first restraint segment 211 and the second restraint segment 212 form a ring structure and are used to be fitted onto the torso of the experimental mouse to restrain the experimental mouse.

[0045] Specifically, the size of the ring structure can be adjusted by changing the connection points between the second restraint segment 212 and the two connecting parts 220 to effectively restrain laboratory mice of different sizes. In actual use, one or both ends of the second restraint segment 212 can be removed from the connecting parts 220 first, then the laboratory mouse can be placed in a suitable position, and then both ends of the second restraint segment 212 can be connected to the two connecting parts 220 respectively to fit the ring structure onto the laboratory mouse's torso to restrain the laboratory mouse, making it convenient to use.

[0046] In some embodiments, such as Figure 2 , Figure 5 As shown, the second binding section 212 is detachably connected to the two connecting parts 220 via Velcro 213.

[0047] Specifically, the two ends of the second restraint segment 212 can be connected to the two connecting parts 220 respectively via Velcro 213. In actual use, the size of the ring structure can be adjusted by adjusting the connection points between the two ends of the second restraint segment 212 and the two connecting parts 220 to effectively restrain experimental mice of different sizes. Preferably, the Velcro 213 on the two connecting parts 220 is preferably textured. When the experimental mouse is large, a ring structure needs to be formed by the first restraint segment 211, the second restraint segment 212, and the connecting parts 220. In this case, the Velcro 213 on the connecting parts 220 is located inside the ring structure. By setting the Velcro 213 on the two connecting parts 220 to textured, the stimulation when it comes into contact with the experimental mouse can be effectively reduced, thus reducing interference with the experiment.

[0048] In some embodiments, such as Figures 1 to 4 As shown, the limiting box 100 includes: an annular body portion 110, and a sliding hole 111 is provided on the limiting box 100;

[0049] The mesh screen 120 covers one end of the main body 110 and is provided with an opening, and a plurality of stimulation holes 121 are provided on the mesh screen 120.

[0050] The first movable part 140 is disposed on the body part 110 and located outside the receiving cavity;

[0051] One of the connecting parts 220 has one end opposite to the binding part 210 passing through the sliding hole 111 and is provided with a second swivel part 230 for detachable connection with the first swivel part 140.

[0052] In actual use, since the connecting part 220 is slidably disposed on the sliding hole 111, the restraining part 210 can be moved away from the limiting box 100. At this time, the connecting part 220 will slide along the sliding hole 111 toward the limiting box 100, so that the restraining part 210 is located outside the limiting box 100, making it easier to operate the restraining part 210 to restrain the experimental mouse. After the experimental mouse is fixed to the limiting box 100, the connecting part 220 can be pulled away from the limiting box 100, so that the restraining part 210 moves toward the limiting box 100 until it is inside the limiting box 100. At this time, the first movable part 140 and the second movable part 230 can be connected, and the experimental mouse can be fixed in a suitable position. During this process, the sliding hole 111 can slide with the connecting part 220, and the restraint strap 200 can also be fixed. Preferably, there are two sliding holes 111, two first movable parts 140, and two second movable parts 230.

[0053] In some embodiments, such as Figures 1 to 4 As shown, the first swivel part 140 is a hook, and the tip of the hook is disposed away from the sliding hole 111. The second swivel part 230 is annular in shape.

[0054] In actual use, the annular second joint 230 can be hooked onto the hook. Since the tip of the hook is away from the sliding hole 111, the first joint 140 and the second joint 230 can be detachably connected.

[0055] In some embodiments, such as Figure 4 As shown, the restraint strap 200 also includes a limiting part 240, which is disposed at one end of the connecting part 220 passing through the sliding hole 111 away from the restraint part 210. The limiting part 240 is used to restrict the connecting part 220 from disengaging from the sliding hole 111.

[0056] In actual use, if the restraint part 210 is moved too far away from the limiting box 100, the connecting part 220 will slide excessively along the sliding hole 111 toward the limiting box 100, causing the second movable part 230 to pass through the sliding hole 111 and enter the limiting box 100. This necessitates subsequently passing the second movable part 230 through the sliding hole 111 to secure the experimental mouse, increasing the steps and reducing efficiency. The limiting part 240 effectively avoids this situation.

[0057] In some embodiments, such as Figures 1 to 2 As shown, the limiting box 100 also includes a cover plate 130, which covers the opening at the other end of the main body 110 and is detachably disposed from the main body 110.

[0058] Preferably, the cover plate 130 is pivotally connected to the top of the limiting box 100, so that the cover plate 130 forms a flip-top cover plate 130 to prevent the laboratory mouse from climbing out.

[0059] In some embodiments, such as Figures 1 to 2 As shown, the cover plate 130 is provided with several ventilation holes 131, which can be used for ventilation of laboratory mice.

[0060] In some embodiments, such as Figure 2 , Figure 5 As shown, the animal restraint device also includes an adjustment buckle 250, which is disposed on the connecting part 220 and is used to adjust the length of the connecting part 220.

[0061] Specifically, by adjusting buckle 250, the length of connecting part 220 can be adjusted according to the size of the experimental mouse, so that the experimental mouse's limbs are attached to the bottom mesh screen.

[0062] In summary, this invention provides a specialized animal restraint device for behavioral testing of lower limb pain in mice. The device includes a restraint strap and a transparent containment box. The containment box has a cavity for accommodating the mouse, and several stimulation holes communicating with the cavity are perforated at the bottom of the box. The restraint strap is placed inside the cavity and connected to the containment box, and is used to restrain the mouse's torso. This invention, by placing the restraint strap inside the containment box, avoids changes in body movement and posture of the mouse during the foot pain behavioral testing process, allowing for testing without requiring the mouse to remain still, thus significantly reducing experimental time. Furthermore, because the mouse's torso is restrained but its limbs are not fixed, its response to pain stimuli is not affected, resulting in accurate and highly repeatable experimental results, greatly improving the accuracy of the experimental data.

[0063] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A specialized animal restraint device for behavioral testing of hind limb pain in rats, characterized in that, include: A transparent limiting box, the limiting box having a receiving cavity for accommodating experimental mice, and the bottom of the limiting box having several stimulation holes communicating with the receiving cavity; A restraint strap is disposed within the receiving cavity and connected to the limiting box; the restraint strap is used to restrain the torso of the experimental mouse. The restraint strap includes a restraint portion, which is used to be fitted onto the torso of the laboratory mouse to restrain the laboratory mouse. Two connecting parts, one end of each connecting part is connected to the two opposite ends of the binding part, and the other end is detachably connected to the limiting box; The limiting box includes: an annular body portion, and the limiting box is provided with a sliding hole; The mesh screen portion covers one end of the main body portion and is provided with an opening, and a plurality of the stimulation holes are provided on the mesh screen portion; A first movable joint is disposed on the body portion and located outside the receiving cavity; One of the connecting portions has one end opposite to the binding portion passing through the sliding hole and is provided with a second swivel portion for detachable connection with the first swivel portion.

2. The animal restraint device for detecting hind limb pain behavior in rats according to claim 1, characterized in that, The binding part includes: a first binding segment, the two opposite ends of the first binding segment being connected to the two connecting parts respectively; The second restraint segment has two opposite ends that are detachably connected to the two connecting parts. The first restraint segment and the second restraint segment form a ring structure and are used to be fitted onto the torso of the experimental mouse to restrain the experimental mouse.

3. The animal restraint device for detecting hind limb pain behavior in rats according to claim 2, characterized in that, The second restraint segment is detachably connected to the two connecting parts via Velcro.

4. The animal restraint device for detecting hind limb pain in rats according to claim 1, characterized in that, The first swivel part is a hook, and the tip of the hook is disposed away from the sliding hole. The second swivel part is annular in shape.

5. The animal restraint device for detecting hind limb pain behavior in rats according to claim 1, characterized in that, The restraint strap further includes a limiting part, which is disposed at one end of the connecting part passing through the sliding hole away from the restraint part, and the limiting part is used to restrict the connecting part from disengaging from the sliding hole.

6. The animal restraint device for detecting hind limb pain behavior in rats according to claim 1, characterized in that, The limiting box further includes a cover plate, which covers the opening at the other end of the main body, and the cover plate is detachably disposed from the main body.

7. The animal restraint device for detecting hind limb pain behavior in rats according to claim 6, characterized in that, The cover plate is provided with several ventilation holes.

8. The animal restraint device for behavioral detection of hind limb pain in rats according to any one of claims 2-7, characterized in that, The animal restraint device further includes an adjustment buckle, which is disposed on the connecting part and is used to adjust the length of the connecting part.

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