Annular reciprocating preparative chromatography column

Abstract

The utility model relates to the technical field of chromatographic column, concretely is a kind of annular reciprocating preparation chromatographic column, including chromatographic column, the chromatographic column is constituted by inner cylinder column and outer cylinder column, the inner cylinder column is located the inside of outer cylinder column, the inner cylinder column and the outer cylinder column form annular column.The utility model, by the chromatographic column is designed as annular, cooperate dispenser simultaneously, to make the path difference of fluid in entering column body small, boundary effect is overcome as far as possible, improve the utilization of chromatographic packing, reduce the column pressure and operating condition of chromatographic column while significantly improve the production capacity of chromatographic column, by the chromatographic column is set as annular reciprocating type, overcome the uneven fluid distribution of conventional column body, volume is too large, column bed instability and other shortcomings, reduce the column pressure and operating condition of chromatographic column while significantly improve the production capacity of chromatographic column, to make the path difference of fluid in entering column body small, boundary effect is overcome as far as possible.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, specifically a ring reciprocating preparative chromatography column. Background Technology

[0002] Industrial chromatography is a large-scale industrial preparation system based on chromatographic separation technology. It separates mixtures by the differential migration of components between the stationary and mobile phases. It adopts a continuous feeding mode and improves preparation efficiency under overload operating conditions. It is widely used in the fields of fine chemicals, biopharmaceuticals and food industry.

[0003] Traditional chromatographic columns are usually cylindrical or conical. In industrial applications, due to scale-up, the column diameter is often higher than 300 mm. There is a time difference between the fluid entering the center point and the boundary of the column, which leads to drawbacks such as uneven fluid distribution, excessive volume, and unstable column bed. Current researchers often focus on the distributor to change its structure so that the diffusion path of the fluid entering the center and boundary of the column is as equal as possible. However, it is difficult to achieve this with current research results.

[0004] Therefore, a ring-shaped reciprocating preparative chromatographic column is proposed to solve the problems mentioned above. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a ring-shaped reciprocating preparative chromatographic column. By designing the chromatographic column as a ring shape and using a distributor, the path difference of the fluid entering the column is reduced, boundary effects are minimized, the utilization rate of the chromatographic packing material is improved, and the column pressure and operating conditions are reduced while significantly increasing the production capacity of the chromatographic column.

[0006] To achieve the above objectives, this utility model provides the following technical solution: including a chromatographic column; The chromatographic column consists of an inner column and an outer column, with the inner column located inside the outer column, and the inner and outer columns forming a ring column.

[0007] Preferably, the inner diameter of the inner cylinder is defined as R_inner, and the range of R_inner is mm to mm.

[0008] Preferably, the inner diameter of the outer cylinder is defined as R_outer, and the range of R_outer is mm-mm.

[0009] Preferably, the range within R is less than the range outside R.

[0010] Preferably, the height of the inner cylinder column is defined as H_inner, and mm ≤ H_inner ≤ mm.

[0011] Preferably, the height of the outer cylinder column is defined as H_outer, and mm ≤ H_inner ≤ mm.

[0012] Preferably, H_inner = H_outer.

[0013] Compared with the prior art, this utility model provides a ring reciprocating preparative chromatographic column, which has the following beneficial effects: 1. By designing the chromatographic column as a ring shape and using a distributor, the path difference of the fluid entering the column is reduced, the boundary effect is overcome as much as possible, the utilization rate of the chromatographic packing is improved, the column pressure and operating conditions of the chromatographic column are reduced, and the production capacity of the chromatographic column is significantly improved.

[0014] 2. By setting the chromatographic column to a reciprocating ring, the shortcomings of conventional columns, such as uneven fluid distribution, excessive volume, and unstable column bed, are overcome. This reduces the column pressure and operating conditions while significantly improving the column's production capacity.

[0015] 3. In conjunction with the distributor, the path difference of the fluid entering the column is reduced, which overcomes the boundary effect as much as possible, improves the utilization rate of the chromatographic packing material, reduces the column pressure and operating conditions of the chromatographic column, and significantly improves the production capacity of the chromatographic column. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the chromatographic column of this utility model; Figure 2 This is a schematic diagram of the inner and outer cylinder structures of this utility model; Figure 3 This is a schematic diagram of the three-dimensional structure of the chromatographic column of this utility model; Figure 4 This is a schematic diagram of the distributor structure of this utility model.

[0017] In the figure: 1, chromatographic column; 101, inner column; 102, outer column. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Example: Please see Figure 1 - Figure 4 In this embodiment, a ring reciprocating preparative chromatographic column includes chromatographic column 1; The chromatographic column 1 consists of an inner cylinder column 101 and an outer cylinder column 102. The inner cylinder column 101 is located inside the outer cylinder column 102, and the inner cylinder column 101 and the outer cylinder column 102 form a ring column. First, the sample loading and elution of the device are reciprocating, that is: the sample is loaded from the column head to the column tail, and after the sample is saturated, the elution is from the column tail to the column head, and the flow rate of the sample loading and elution should be 1-10 BV / h. The sample loading amount is the actual field test result, and the elution amount is equal to the sample loading amount; Furthermore, the connection method and connection position of this device and other corresponding components are the same as those of existing devices, and belong to the well-known technology of those skilled in the art. Therefore, they are not described in detail in this embodiment. By setting the chromatographic column to a ring reciprocating type, the shortcomings of conventional columns, such as uneven fluid distribution, excessive volume, and unstable column bed, are overcome. The column pressure and operating conditions of the chromatographic column are reduced while the production capacity of the chromatographic column is significantly improved. Simultaneously, the distributor reduces the path difference of fluid entering the column, minimizing boundary effects, improving the utilization rate of chromatographic packing material, and significantly increasing the column production capacity while reducing column pressure and operating conditions.

[0020] Example 1: The inner diameter of the inner cylinder column 101 is defined as R_inner, and the range of R_inner is 300mm-2000mm; The inner diameter of the outer cylinder 102 is defined as R_outer, and the range of R_outer is 300mm-2000mm; The range within R < the range outside R; The column height of the inner cylinder column 101 is defined as H_inner, and 200mm ≤ H_inner ≤ 1600mm; The height of the outer cylinder column 102 is defined as H_outer, and 200mm ≤ H_inner ≤ 1600mm; H_inner = H_outer; Option 1: Set column 1 to a ring column with R inside 300mm, R outside 500mm, and H inside and H outside both 200mm; (1) The waste liquid from the aluminum profile oxidation tank after the pretreatment system is loaded onto a chromatographic column with industrial chromatographic separation material at a loading flow rate of 6 BV / h.

[0021] (2) Elution: Pure water is used to elute from the other end of the reciprocating flow low bed. The eluent is recycled acid that is directly returned to the anodic oxidation tank. The eluent is water. The flow rate of the eluent is 6 BV / h.

[0022] Column pressure: 0.15 MPa, single-cycle throughput: 1 BV Option 2: Set column 1 to a ring column with R inside 600 mm, R outside 1000 mm, and H inside and H outside both 400 mm. (1) The waste liquid from the aluminum profile oxidation tank after the pretreatment system is loaded onto a chromatographic column with industrial chromatographic separation material at a loading flow rate of 6 BV / h.

[0023] (2) Elution: Pure water is used to elute from the other end of the reciprocating flow low bed. The eluent is recycled acid that is directly returned to the anodic oxidation tank. The eluent is water. The flow rate of the eluent is 6 BV / h.

[0024] Column pressure: 0.12 MPa, single-cycle throughput: 3 / 4 BV.

[0025] Option 3: Set column 1 to a ring column with R inside 1200 mm, R outside 2000 mm, and H inside and H outside both 800 mm; (1) The waste liquid from the aluminum profile oxidation tank after the pretreatment system is loaded onto a chromatographic column with industrial chromatographic separation material at a loading flow rate of 6 BV / h.

[0026] (2) Elution: Pure water is used to elute from the other end of the reciprocating flow low bed. The eluent is recycled acid that is directly returned to the anodic oxidation tank. The eluent is water. The flow rate of the eluent is 6 BV / h.

[0027] Column pressure: 0.13 MPa, single-cycle throughput: 2 / 3 BV.

[0028] Traditional approach: Construct column 1 as a ring column with a diameter of 400 mm and a height of 200 mm. (Column volume is the same as in Scheme 1 above.) (1) The waste liquid from the aluminum profile oxidation tank after the pretreatment system is loaded onto a chromatographic column with industrial chromatographic separation material at a loading flow rate of 6 BV / h.

[0029] (2) Elution: Pure water is used to elute from the other end of the reciprocating flow low bed. The eluent is recycled acid that is directly returned to the anodic oxidation tank. The eluent is water. The flow rate of the eluent is 6 BV / h.

[0030] Column pressure: 0.3 MPa, single-cycle throughput: 1 / 3 BV.

[0031] By comparing the above-mentioned Scheme 1 with the traditional scheme, under the same sample loading flow rate and elution, we obtained data on column pressure and single cycle volume that are completely different. Moreover, the scheme used in this device has more efficient and faster cycle processing.

[0032] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0033] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A ring-shaped reciprocating preparative chromatographic column, characterized in that: Includes chromatographic column (1); The chromatographic column (1) is composed of an inner cylinder column (101) and an outer cylinder column (102). The inner cylinder column (101) is located inside the outer cylinder column (102), and the inner cylinder column (101) and the outer cylinder column (102) form a ring column.

2. The ring-shaped reciprocating preparative chromatographic column according to claim 1, characterized in that: The inner diameter of the inner cylinder (101) is defined as R_inner, and the range of R_inner is 300mm-2000mm.

3. The ring-shaped reciprocating preparative chromatographic column according to claim 2, characterized in that: The inner diameter of the outer cylinder (102) is defined as R_outer, and the range of R_outer is 300mm-2000mm.

4. The ring-shaped reciprocating preparative chromatographic column according to claim 3, characterized in that: The range within R is less than the range outside R.

5. A ring-shaped reciprocating preparative chromatographic column according to claim 1, characterized in that: The height of the inner cylinder column (101) is defined as H_inner, and 200mm ≤ H_inner ≤ 1600mm.

6. The ring-shaped reciprocating preparative chromatographic column according to claim 5, characterized in that: The height of the outer cylinder column (102) is defined as H_outer, and 200mm ≤ H_inner ≤ 1600mm.

7. A ring-shaped reciprocating preparative chromatographic column according to claim 6, characterized in that: H inside = H outside.

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