A cell liquid mixing device

Abstract

The utility model relates to cell culture equipment field, concretely is a kind of cell liquid mixing device, it includes oblique mixing part, eccentric part and power part, the power part drives eccentric part action to drive oblique mixing part eccentric rotation;The oblique mixing part includes mixing plate, pressing plate, at least one vertical rod and the upper support plate of horizontal arrangement;The vertical rod is vertically arranged on the upper support plate, the lower end of mixing plate is installed on the upper support plate, upper end is installed on vertical rod to present the oblique state of upper high lower low, and the upper support plate is connected eccentric part;The pressing plate is installed on the upper surface of mixing plate and can be released or pressed tightly liquid storage bag between the two. This mixing device can be oblique eccentric shake cell liquid, saves manpower, realizes more efficient, higher quality cell liquid mixing.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment, specifically a cell fluid mixing device. Background Technology

[0002] In the cell and gene therapy industry, the formulation packaging process requires the thorough mixing of three liquids: cell solution, diluent, and cryopreservation solution, before further packaging to create smaller final product bags. Common sizes for these storage bags include 5ml and 350ml, and they are typically made of EVA or PE, offering features such as aseptic packaging and cryogenic storage. Current methods for mixing cell solution are usually manual, involving manually squeezing the bag to achieve mixing, which is inefficient. Summary of the Invention

[0003] To address the aforementioned problems, this invention provides a cell fluid mixing device that uses eccentric shaking to automatically mix the cell fluid. The technical solution adopted by this invention is as follows: A cell fluid mixing device includes an inclined mixing section, an eccentric section, and a power unit. The power unit drives the eccentric section to rotate the inclined mixing section eccentrically. The inclined mixing section includes a mixing plate, a pressure plate, at least one upright rod, and a horizontally arranged upper support plate. The upright rod is vertically mounted on the upper support plate. The lower end of the mixing plate is mounted on the upper support plate, and the upper end is mounted on the upright rod, forming an inclined state with the upper end higher than the lower end. The upper support plate is connected to the eccentric section. The pressure plate is mounted on the upper surface of the mixing plate, and the two can be loosened or tightened to form a storage bag.

[0004] In the above-mentioned cell fluid mixing device, one end of the pressure plate is hinged to the mixing plate, and the other end is pluggable and detachable from the mixing plate through a snap-fit ​​mechanism.

[0005] The cell fluid mixing device described above has an adjustable mounting plate on the mixing plate. The two ends of the pressure plate are fitted with the mounting plate to adjust the position on the mixing plate.

[0006] In the above-mentioned cell fluid mixing device, the mounting plate is slidably mounted on the mixing plate.

[0007] In the above-mentioned cell fluid mixing device, linear slide rails are provided on the left and right sides of the lower surface of the mixing plate, and a slider is provided on each linear slide rail. The sliders are respectively installed at both ends of the mounting plate. The mounting plate has mounting blocks on its left and right sides, and the mixing plate has sliding grooves on its left and right sides corresponding to the positions of the mounting blocks. The mounting block on the left side is locked in the left sliding groove and hinged to the left end of the pressure plate, while the mounting block on the right side is locked in the right sliding groove and connected to the pressure plate through a snap-fit ​​mechanism.

[0008] In the above-mentioned cell fluid mixing device, an elastic gasket is installed on the pressing surface of the pressure plate.

[0009] In the above-mentioned cell fluid mixing device, the latching mechanism is a plug-in type bead-matching mechanism.

[0010] The above-mentioned cell fluid mixing device includes an eccentric part comprising a rotating shaft, a turntable, an eccentric sleeve, and a lower support plate. The power unit drives the rotating shaft, which in turn drives the turntable to rotate. The eccentric sleeve is eccentrically mounted on the upper surface of the turntable. The lower support plate is mounted on the eccentric sleeve. The upper support plate is connected to the lower support plate to eccentrically sway.

[0011] The aforementioned cell fluid mixing device further includes a base and a buffer mechanism in the eccentric part. The base is horizontally positioned above the power unit, and the buffer mechanism includes four squarely arranged buffer plates and four squarely distributed reinforcing rods. The buffer plates and reinforcing rods are connected to form a "U"-shaped buffer mechanism, and the ends of two of the buffer plates are connected to lower support rods.

[0012] In the above-mentioned cell fluid mixing device, a counterweight is installed on the lower surface of the turntable, and the position of the counterweight is collinear with the position of the eccentric sleeve in the radial direction.

[0013] The beneficial effects of this invention are as follows: This mixing device can tilt and eccentrically shake the cell solution, saving manpower and achieving higher efficiency and higher quality cell solution mixing. Attached Figure Description

[0014] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model (in a compressed state). Figure 2 This is a structural schematic diagram of an embodiment of the present utility model (in the released state). Figure 3 This is a structural schematic diagram from the rear view of an embodiment of the present invention; Figure 4 and Figure 5 This is a schematic diagram of the pressure plate in an embodiment of the present utility model; Figure 6 and Figure 7 This is a schematic diagram of the cam mechanism according to an embodiment of the present invention.

[0015] In the diagram: 1 is the mixing plate, 2 is the pressure plate, 3 is the upright, 4 is the upper support plate, 5 is the base, 6 is the power unit, 7 is the mounting plate, 8 is the linear slide rail, 9 is the turntable, 10 is the counterweight, 11 is the eccentric sleeve, 12 is the buffer plate, 13 is the reinforcing rod, 14 is the pad, 15 is the lower support plate, 16 is the slide groove, 17 is the toothed baffle, 20 is the liquid storage bag, 21 is the handle, 22 is the protrusion, 23 is the locking block, 24 is the elastic pad, 61 is the heat insulation pad, 71 is the locking seat, 72 is the mounting block, and 81 is the slider. Detailed Implementation

[0016] The technical content of this utility model will be described in detail below with reference to the accompanying drawings. Unless otherwise specified, the components such as motors and couplings in the following embodiments are all commercially available products, and various sheet metal parts are custom-made by manufacturers according to the drawings.

[0017] This embodiment is a cell fluid mixing device, including an inclined mixing section, an eccentric section, and a power unit 6, as shown below. Figures 1 to 7 The inclined mixing section includes a mixing plate 1, a pressure plate 2, two uprights 3, and a horizontally arranged upper support plate 4. The uprights 3 are vertically mounted on the upper support plate 4. The lower end of the mixing plate 1 is installed on the upper support plate 4, and the upper end is supported by the uprights 3, resulting in an inclined state. The pressure plate 2 is installed on the upper surface of the mixing plate 1, and the two can be loosened or tightened. The upper support plate 4 is driven by an eccentric part to rotate eccentrically in the horizontal plane, and the eccentric part is driven by a power unit 6. In use, the storage bag 20 containing cell fluid is placed on the mixing plate 1 and pressed tightly by the pressure plate 2. The storage bag 20 can be shaken evenly in an inclined state, resulting in high mixing efficiency. Most storage bags 20 have an infusion tube at the head and a plastic seal at the tail, such as the Nipro culture bag, which is made of ultra-low density PE. Some are plastic sealed at both ends, with the infusion tube located in the middle of the bag. The models and specifications produced by different manufacturers are mostly different, and users can customize according to their needs. When in use, the pressure plate 2 presses firmly against the plastic seal of the storage bag 20, with the plastic seal facing upwards and the infusion tube facing downwards. The pressure plate 2 and the mixing plate 1 are completely pressed together (interference fit) without any gaps to prevent the storage bag 20 from slipping off. For example, the pressure plate 2 can be magnetically attached to the mixing plate 1 (choose a material with strong magnetic force, as the thin storage bag 20 will hardly weaken the magnetic force), or the two can be mechanically connected.

[0018] In this embodiment, the pressure plate 2 and the mixing plate 1 are mechanically connected. Specifically, one end of the pressure plate 2 is hinged to the mixing plate 1, and the other end is plugged into or snapped into the mixing plate 1 via a snap-fit ​​mechanism, which facilitates the operation of loosening or tightening the liquid storage bag 20. To ensure the tightening effect and prevent the liquid storage bag 20 from slipping during the mixing action, elastic gaskets 24, such as EVA foam, can be installed (by means of bonding, etc.) on the pressing surface of the pressure plate 2 or on the mixing plate 1. The elasticity increases the squeezing force, which can effectively prevent the liquid storage bag 20 from slipping and avoid scratching the liquid storage bag 20.

[0019] The latching mechanism uses a plug-in type ball-operated mechanism, where a spring-loaded ball acts as a latch 71 mounted on the mixing plate 1, and a mating block acts as a latch 23 mounted on the pressure plate 2. During the mixing action, the mixing plate 1 oscillates within its own plane, without oscillation perpendicular to the plate surface. Therefore, the latching mechanism is firmly engaged and will not disengage. Actual machine testing has also confirmed the reliability of this latching mechanism. Both the spring-loaded ball and the mating block are standard parts, and commercially available products can be used directly.

[0020] Furthermore, to accommodate liquid storage bags 20 of different sizes and specifications, an adjustable mounting plate 7 is provided on the mixing plate 1. The two ends of the pressure plate 2 are fitted with the mounting plate 7 to adjust its position on the mixing plate 1, thereby adjusting the position of the liquid storage bag 20 on the mixing plate 1. Specifically, in conjunction with... Figure 3 Linear slide rails 8 are provided on the left and right sides of the lower surface of the mixing plate 1. Each linear slide rail 8 is equipped with a slider 81. The two ends of the mounting plate 7 are respectively installed with the sliders 81. Mounting blocks 72 are provided on the left and right sides of the mounting plate 7. Slide grooves 16 are provided on both sides of the mixing plate 1 corresponding to the positions of the mounting blocks 72. The mounting block 72 on the left side is engaged in the left slide groove 16 and is hinged to the left end of the pressure plate 2. The mounting block 72 on the right side is engaged in the right slide groove 16 and is equipped with a mounting bracket 71 to match the right end locking block 23 of the pressure plate 2. By adjusting the position of the pressure plate 2, the center of gravity of the liquid storage bag 20 of any size can be kept in the middle of the mixing plate 1, ensuring the uniformity of mixing.

[0021] Furthermore, to limit the position of the pressure plate 2 on the mixing plate 1, a limiting mechanism is also needed to prevent the pressure plate 2 or the mounting plate 7 from sliding arbitrarily. In this embodiment, a groove-shaped limiting mechanism is provided for the pressure plate 2. Specifically, a protrusion 22 is provided at the snap-fit ​​end, i.e., the right end, of the pressure plate 2. Correspondingly, a toothed baffle 17 is installed on the right side of the upper surface of the mixing plate 1. The toothed baffle 17 has several vertically distributed grooves for locking the protrusion 22 (or the right end of the pressure plate 2 can be set as a groove, corresponding to the teeth of the toothed baffle 17). In use, according to the specifications of the liquid storage bag 20, the pressure plate 2 is adjusted to the required position, and the pressure plate 2 is pressed against the liquid storage bag 20 by the snap-fit ​​mechanism. At the same time, the protrusion 22 is locked in a groove on the toothed baffle 17 to limit its position. Other conventional limiting mechanisms can also be used here. For example, a pin-type limiting mechanism can be set for the mounting plate 7. Specifically, a bolt is screwed onto the slider 81, and several vertically distributed screw holes are set on the linear slide rail 8. When limiting, the bolt is tightened so that it enters a screw hole on the linear slide rail 8. When the position needs to be adjusted, the bolt is loosened so that it is disengaged from the linear slide rail 8. Alternatively, a pin-type limiting mechanism can be set for the pressure plate 2. Specifically, a vertical pin is placed on the right end of the pressure plate 2 (the pin axis is perpendicular to the surface of the pressure plate 2), and several vertically distributed slots (which can be elongated) are set on the right side of the mixing plate 1. When limiting, when the pressure plate 2 is pressed against the mixing plate 1, the pin is inserted into the slot, thus limiting the position of the pressure plate 2.

[0022] Combination Figure 3 , Figure 6 and Figure 7The eccentric part includes a rotating shaft, a turntable 9, an eccentric sleeve 11, and a lower support plate 15. The power unit 6 drives the rotating shaft, which in turn drives the turntable 9 to rotate. The eccentric sleeve 11 is eccentrically mounted on the upper surface of the turntable 9. The eccentric distance between the turntable 9 and the eccentric sleeve 11 is set to 10mm. The lower support plate 15 is mounted on the eccentric sleeve 11. The upper support plate 4 is connected to the lower support plate 15 to eccentrically sway.

[0023] The eccentric portion also includes a base 5 and a buffer mechanism. The base 5 includes a horizontal mounting plate and several aluminum profiles as support legs, as can be seen from [reference needed]. Figure 3 A heat insulation pad 61 is provided between the mounting plate and the support legs. The base 5 contains a power unit 6, which is a pre-existing product, including necessary components such as a motor and coupling, used to provide mixing power. Figure 7 As shown, the buffer mechanism includes four square-arranged buffer plates 12 and four square-distributed reinforcing rods 13. The buffer plates 12 and reinforcing rods 13 are connected to form an approximately "U"-shaped buffer mechanism. (Refer to...) Figure 6 Both buffer plates 12 have pads 14 at their ends to connect to the lower support plate 15. This can buffer the lower support plate 15 when it rotates eccentrically in four directions, thus making the shaking of the mixing plate 1 more stable.

[0024] like Figure 3 As shown, a counterweight 10 is installed on the lower surface of the turntable 9. The position of the counterweight 10 is collinear with the position of the eccentric sleeve 11 in the radial direction, which serves as a counterweight for the eccentric part.

Claims

1. A cell fluid mixing device, characterized in that: It includes an inclined mixing section, an eccentric section and a power section (6), wherein the power section (6) drives the eccentric section to rotate the inclined mixing section eccentrically; The inclined mixing section includes a mixing plate (1), a pressure plate (2), at least one upright (3), and a horizontally arranged upper support plate (4); the upright (3) is vertically arranged on the upper support plate (4), the lower end of the mixing plate (1) is installed on the upper support plate (4), and the upper end is installed on the upright (3) in an inclined state with the upper end higher than the lower end; the upper support plate (4) is connected to the eccentric part; the pressure plate (2) is installed on the upper surface of the mixing plate (1), and the two can be loosened or pressed together to form a liquid storage bag (20).

2. The cell fluid mixing device according to claim 1, characterized in that: One end of the pressure plate (2) is hinged to the mixing plate (1), and the other end is pluggable and detachable from the mixing plate (1) through a snap-fit ​​mechanism.

3. The cell fluid mixing device according to claim 2, characterized in that: The mixing plate (1) is provided with an adjustable mounting plate (7). The two ends of the pressure plate (2) are installed in correspondence with the mounting plate (7) to adjust the position on the mixing plate (1).

4. The cell fluid mixing device according to claim 3, characterized in that: The mounting plate (7) is slidably mounted on the mixing plate (1).

5. The cell fluid mixing device according to claim 3, characterized in that: The mixing plate (1) has linear slide rails (8) on its lower surface on both the left and right sides, and each linear slide rail (8) is equipped with a slider (81). The two ends of the mounting plate (7) are respectively equipped with sliders (81). The mounting plate (7) has mounting blocks (72) on its left and right sides. The mixing plate (1) has grooves (16) on its left and right sides corresponding to the mounting blocks (72). The mounting block (72) on the left side is locked in the left groove (16) and hinged to the left end of the pressure plate (2). The mounting block (72) on the right side is locked in the right groove (16) and connected to the pressure plate (2) through a snap-fit ​​mechanism.

6. The cell fluid mixing apparatus according to any one of claims 2 to 5, characterized in that: An elastic gasket (24) is installed on the pressing surface of the pressure plate (2).

7. The cell fluid mixing device according to claim 6, characterized in that: The latching mechanism is a plug-in type ball-operated mechanism.

8. The cell fluid mixing device according to claim 1, characterized in that: The eccentric part includes a rotating shaft, a turntable (9), an eccentric sleeve (11), and a lower support plate (15). The power unit (6) drives the rotating shaft, which drives the turntable (9) to rotate. The eccentric sleeve (11) is eccentrically mounted on the upper surface of the turntable (9). The lower support plate (15) is mounted on the eccentric sleeve (11). The upper support plate (4) is connected to the lower support plate (15) to eccentrically sway.

9. The cell fluid mixing device according to claim 8, characterized in that: The eccentric part also includes a base (5) and a buffer mechanism. The base (5) is horizontally arranged above the power unit (6). The buffer mechanism includes four square-arranged buffer plates (12) and four square-distributed reinforcing rods (13). The buffer plates (12) and reinforcing rods (13) are connected to form a "U"-shaped buffer mechanism. The ends of two of the buffer plates (12) are connected to a lower support plate (15).

10. The cell fluid mixing device according to claim 8, characterized in that: A counterweight (10) is installed on the lower surface of the turntable (9), and the position of the counterweight (10) is collinear with the position of the eccentric sleeve (11) in the radial direction.

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