A polypeptide protein mixing device

Abstract

The utility model provides a kind of polypeptide protein mixing device, including mixing kettle body, the upper of the mixing kettle body is provided with at least three injection pipe orifice, and its lower end is provided with discharge pipe orifice;Mixing kettle body is installed with inside and outside circulation mixing, inside and outside circulation mixing is connected with the corresponding circulation pipe orifice of mixing kettle body by four circulating pumps;The upper end of the stirring shaft rotationally installed in mixing kettle body is fixed with the output end of driving part, and driving part is fixedly installed on the upper outside of mixing kettle body;The utility model circulating pump controls Y type external mixing pipeline, and material is premixed by being drawn from the kettle body both sides, and is sent annular built-in injection channel by external pipeline, and injection head sprays material towards stirring shaft, and material is centrifuged into fine liquid drop and covers full domain, reduces concentration difference, increases contact area, and shortens mixing time.

Description

Technical Field

[0001] This invention belongs to the field of mixing technology, and particularly relates to a polypeptide protein mixing device. Background Technology

[0002] In the field of peptide protein production and processing (covering biopharmaceuticals, functional foods, feed additives, etc.), the mixing process is the core link that determines the purity, activity and uniformity of the product. Peptide protein molecules are prone to aggregation due to excessively high local concentrations and denaturation due to temperature fluctuations. In addition, the finished product must meet strict standards for component uniformity. Therefore, extremely high requirements are placed on the performance of the mixing equipment.

[0003] Currently, mainstream peptide-protein mixing devices are mainly divided into two categories, but both have unavoidable technical defects: Limitations of single-shaft mechanical mixing devices: Such devices rely on the rotation of the stirring shaft to achieve material mixing. Although they are simple in structure and low in cost, they are physically limited by the stirring range, and "mixing dead zones" are easily formed inside the vessel. The material near the vessel wall and bottom is difficult to be stirred by the stirring shaft, which results in the peptide powder and protein concentrate not being able to fully contact each other. Some peptide powders form clumps due to untimely dissolution (especially short-chain peptide powders with small particle size and large specific surface area, which are easy to adsorb and agglomerate). This not only affects the uniformity of the finished product, but may also clog subsequent filtration or filling equipment.

[0004] Limitations of single-pipe flow mixing devices: To address the issue of dead zones in mixing, some devices employ a "circulating pump + single pipeline" flow mixing mode. The circulating pump extracts material from the bottom of the vessel and sprays it onto the top of the vessel through a pipeline to achieve circulation. However, this type of device can only extract and circulate a "single stream of material," and the material on the sides and below the sides of the vessel cannot be cross-exchanged, creating new "circulation dead zones."

[0005] Therefore, it is essential to invent a polypeptide-protein mixing device. Utility Model Content

[0006] To solve the above-mentioned technical problems, this utility model provides a polypeptide protein mixing device, including a mixing vessel, a feeding port, a discharging port, an internal and external circulation mixer, a circulation pump, a circulation port, a stirring shaft, and a driving component. At least three feeding ports are provided on the upper part of the mixing vessel, and the discharging port is provided at its lower end. The internal and external circulation mixer is installed on the mixing vessel, and the internal and external circulation mixer is connected to the corresponding circulation ports provided on the mixing vessel through four circulation pumps. The upper end of the stirring shaft, which is rotatably installed inside the mixing vessel, is fixed to the output end of the driving component, and the driving component is fixedly installed on the upper part of the outside of the mixing vessel.

[0007] Preferably, the internal and external circulation mixing includes a built-in injection channel, injection heads, external pipes, an external mixing pipe, a steam heating box, and a steam port. Several injection heads are arranged in a circular array below the built-in injection channel. The built-in injection channel is connected to the corresponding external mixing pipe through two external pipes, and the external pipes are fixedly passed through the mixing vessel. The steam heating box fixedly installed on the external mixing pipe is connected to a high-temperature steam generator through a pipe and the steam port.

[0008] Preferably, the built-in injection channel is located inside the mixing vessel and above the stirring shaft. The built-in injection channel is an annular pipe with its center overlapping the axis of the stirring shaft. Several injection heads located below the built-in injection channel are inclined and their injection outlets face the stirring shaft.

[0009] Preferably, the built-in injection channel is connected to the upper vertical part of the two inverted "Y"-shaped external mixing pipes through two external pipes. The external mixing pipes are located outside the mixing vessel. The two branches below the external mixing pipes are connected to the corresponding circulation ports on the mixing vessel through the circulation pump.

[0010] Preferably, each of the circulation ports of the mixing vessel is equipped with a filter screen. The circulation pump can draw the raw material from the bottom of the mixing vessel into the external mixing pipe, and then flow through the external pipe into the built-in injection channel, and then spray it onto the surface above the stirring shaft through a number of injection heads set in the built-in injection channel.

[0011] Preferably, a steam heating box is fixedly installed on the outside of the vertical part of the external mixing pipe, and the two steam ports provided on the steam heating box are an exhaust port and an injection port, respectively.

[0012] Compared with the prior art, the present invention has the following beneficial effects: The two branches of the external mixing pipe of this utility model draw materials from the lower sides of the vessel body (by the coordinated control of 4 circulating pumps, the materials on both sides are drawn evenly). After premixing in the vertical part of the Y-shaped pipe, the materials are transported to the annular built-in spray channel through the external pipe. The built-in spray channel is annular (the center overlaps with the axis of the stirring shaft). Several spray heads are set at the bottom and face the stirring shaft. After the materials are sprayed onto the surface of the rotating stirring shaft, they are thrown into fine droplets by the centrifugal force of the stirring shaft and evenly cover the entire area of ​​the vessel body.

[0013] This utility model features a Y-shaped external mixing pipe that enables "dual-strand material premixing," reducing the "initial concentration difference" in the mixing process within the vessel. On the other hand, the synergistic effect of the annular spray head and the stirring shaft significantly increases the material contact area, shortens the material mixing time, and improves production efficiency. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0015] Figure 2 This is a half-sectional structural schematic diagram of the present invention.

[0016] Figure 3 This is a schematic diagram of the structure of the present invention, which is a hybrid internal and external circulation system before installation.

[0017] Figure 4 This is a schematic diagram of the internal and external circulation hybrid structure of this utility model.

[0018] In the picture: 1. Mixing vessel body; 2. Injection port; 3. Discharge port; 4. Internal and external circulation mixing system; 41. Built-in injection channel; 42. Injection head; 43. External pipe; 44. External mixing pipe; 45. Steam heating box; 46. Steam port; 5. Circulation pump; 6. Circulation port; 7. Stirring shaft; 8. Drive components. Detailed Implementation

[0019] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0020] In the description of the embodiments, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the present invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of the utility model, it should be noted that unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in the present utility model based on the specific circumstances.

[0021] As attached Figure 1 To be continued Figure 4 As shown: This utility model provides a polypeptide protein mixing device, including a mixing vessel 1, a feeding port 2, a discharging port 3, an internal and external circulation mixing 4, a circulation pump 5, a circulation port 6, a stirring shaft 7, and a driving component 8. At least three feeding ports 2 are provided on the upper part of the mixing vessel 1, and the discharging port 3 is provided on its lower end. The internal and external circulation mixing 4 is installed on the mixing vessel 1, and the internal and external circulation mixing 4 is connected to the corresponding circulation ports 6 provided on the mixing vessel 1 through four circulation pumps 5. The upper end of the stirring shaft 7, which is rotatably installed inside the mixing vessel 1, is fixed to the output end of the driving component 8, and the driving component 8 is fixedly installed on the upper part of the outside of the mixing vessel 1.

[0022] Furthermore, the internal and external circulation mixing 4 is made of food-grade 316L stainless steel to meet hygiene requirements. It includes an internal spray channel 41, a spray head 42, an external pipe 43, an external mixing pipe 44, a steam heating box 45, and a steam port 46. The built-in injection channel 41 is a hollow annular pipe structure, with 6-12 injection heads 42 evenly arranged in a circular array below it along the circumference. The injection heads 42 are connected to the built-in injection channel 41 by a threaded seal for easy disassembly and cleaning. The built-in injection channel 41 is connected to the corresponding external mixing pipe 44 through two symmetrically distributed external pipes 43. The external pipes 43 are connected to the side wall of the mixing vessel 1 through a sanitary quick-install flange, and a food-grade silicone sealing ring is set at the connection to ensure sealing performance. A steam heating box 45 is fixedly installed on the outer side of the middle of the external mixing pipe 44 by welding. The steam heating box 45 has a double-layer jacket structure, and one side of it is connected to the steam port 46 through a seamless steel pipe. The steam port 46 is connected to the output end of the external high-temperature steam generator through a high-pressure hose, forming a complete heating medium circulation path.

[0023] Furthermore, the built-in injection channel 41 is horizontally fixed inside the mixing vessel 1, positioned above the stirring shaft 7, with a vertical distance of 30-50cm between them to ensure effective injection. This built-in injection channel 41 employs a ring-shaped pipe design with an inner diameter of 30-60cm. The ring diameter is 1 / 3-1 / 2 of the inner diameter of the mixing vessel 1, and the center of the ring completely overlaps with the rotation axis of the stirring shaft 7, ensuring symmetrical material injection. Several injection heads 42 located below the built-in injection channel 41 all adopt an inclined structure design, with their injection axes forming an angle of 30°-45° with the vertical direction. The injection outlets of all injection heads 42 face the upper surface of the stirring shaft 7, forming a converging injection area to ensure that the ejected material directly impacts the rotating stirring shaft 7.

[0024] Furthermore, the built-in injection channel 41 is connected to two independently installed external mixing pipes 44 via two external pipes 43 of equal diameter. One end of the external pipe 43 is fixed to the built-in injection channel 41 by welding, and the other end is detachably connected to the upper vertical part of the external mixing pipe 44 via a flange. The external mixing pipe 44 has an inverted "Y" shape and is made of 316L stainless steel seamless pipe. The angle between the two branches and the vertical part is 60°-90° to ensure smooth material mixing. The external mixing pipe 44 is symmetrically distributed on both sides of the outside of the mixing vessel 1. The ends of the two branches below are connected to the output end of the circulation pump 5 through a sanitary centrifugal pump interface. The input end of the circulation pump 5 is sealed and connected to the corresponding circulation port 6 set on the lower side wall of the mixing vessel 1 through a pipe, forming a double-sided symmetrical material circulation path.

[0025] Furthermore, each circulation port 6 located below the side wall of the mixing vessel 1 is a flange-type interface structure, with a 100-200 mesh stainless steel filter screen fixed inside by bolts. A sealing ring is set between the edge of the filter screen and the inner wall of the port, which can effectively intercept undissolved solid particles or impurities in the vessel, preventing blockage of the circulation pump 5 and the pipeline, while ensuring the smooth passage of liquid materials. During operation, the circulation pump 5 generates negative pressure through motor drive, drawing the liquid raw material from the lower part of the mixing vessel 1 from the circulation port 6. After being filtered by the filter screen, it is pumped into the two branches of the external mixing pipeline 44. The two materials are initially combined and mixed in the vertical part of the "Y"-shaped external mixing pipeline 44, and then transported to the built-in spray channel 41 through the external pipeline 43. Finally, a high-speed jet is formed by several inclined spray heads 42 located below the built-in spray channel 41, which is directionally sprayed onto the surface above the rotating stirring shaft 7 to achieve secondary dispersion and mixing of the materials.

[0026] Furthermore, a steam heating box 45 is fixedly fitted onto the vertical part of the external mixing pipe 44 by welding. This steam heating box 45 is a sealed cavity structure, with a 5-10mm gap between its inner wall and the outer wall of the external mixing pipe 44 to form a heating chamber. A steam injection port is located on the lower side wall of the steam heating box 45, and a steam outlet is located on the upper side (connected to a steam recovery unit via a pipe). Both ports are threaded. The injection port is connected to a high-temperature steam generator via a pipe, forming a complete steam heating circulation system. When heating of the circulating material is required, high-temperature steam enters the heating chamber through the injection port and exchanges heat with the outer wall of the external mixing pipe 44, achieving indirect heating of the material inside the pipe and avoiding localized overheating caused by direct contact between the material and the heat source.

[0027] The working principle is as follows: First, according to the requirements of the polypeptide protein mixing process, polypeptide powder and protein concentrate (or protein raw material) are added into the vessel through at least three injection ports 2 above the mixing vessel 1, and water (or buffer solution) is added as needed to adjust the overall viscosity of the material to ensure subsequent flowability; at the same time, it is checked that the discharge port 3 is in the closed state to avoid material leakage.

[0028] Next, the drive component 8, which is fixed on the outside of the mixing vessel 1, is started. The drive component 8 drives the stirring shaft 7, which is installed inside the vessel, to rotate at a low speed (20-50 rpm). The stirring shaft 7 uses its own blades to initially stir the material inside the vessel, fully dispersing the peptide powder, protein concentrate, and water (or buffer solution) to gradually form a uniform liquid material, laying the foundation for subsequent cyclic mixing.

[0029] Then, start the four circulation pumps 5 connected to the circulation port 6 below the side wall of the mixing vessel 1. The circulation pumps 5 generate negative pressure through motor drive, and draw the liquid material in the lower part of the vessel out from the circulation port 6. At this time, the 100-200 mesh stainless steel filter screen in the circulation port 6 will intercept the undissolved solid particles (such as peptide clumps) to prevent them from entering the pipeline and clogging the equipment. The filtered material is pumped into the two branches of the inverted "Y"-shaped external mixing pipe 44 on both sides of the outside of the mixing vessel 1.

[0030] Next, the two materials are initially combined and mixed in the vertical part of the "Y"-shaped external mixing pipe 44 to eliminate local concentration differences. If the materials need to be heated (such as poorly soluble peptides needing to be dissolved more quickly), high-temperature steam is introduced through the steam injection port of the steam heating box 45. The steam exchanges heat with the outer wall of the pipe in the heating chamber of the steam heating box 45 (the gap between the inner wall and the outer wall of the external mixing pipe 44 is 5-10 mm), indirectly heating the materials in the pipe. The heated materials are then transported through the external pipe 43 to the annular built-in injection channel 41 above the inside of the mixing vessel 1.

[0031] Subsequently, the material is evenly distributed within the annular built-in spray channel 41, and forms a high-speed jet through 6-12 inclined spray heads 42 arranged in a circular array below (at an angle of 30°-45° with the vertical direction), which are directionally sprayed onto the upper surface of the rotating stirring shaft 7 directly below. After impacting the stirring shaft 7, the material is flung into fine droplets of 0.5-2mm by the centrifugal force of the stirring shaft 7, which are evenly diffused throughout the entire mixing vessel 1, and are thoroughly mixed again with the original material in the vessel, completing one cycle of "extraction-premixing-spraying-dispersion".

[0032] Finally, repeat the material circulation driven by the circulating pump 5 and the stirring process of the stirring shaft 7 to continuously enhance the mixing effect. If it is necessary to add subsequent raw materials (such as stabilizers and excipients), they can be added at any time through the injection port 2 until the material mixing uniformity meets the process requirements (such as deviation ≤ ±1.5%). Then, turn off the drive component 8 and the circulating pump 5, open the discharge port 3, and discharge the mixed peptide protein material to complete the entire mixing operation.

[0033] Any technical solution that achieves the above-mentioned technical effects by utilizing the technical solution described in this utility model, or by designing a similar technical solution inspired by the technical solution described in this utility model, falls within the protection scope of this utility model.

Claims

1. A polypeptide-protein mixing device, characterized in that, The mixing vessel includes a mixing tank (1), a feeding port (2), a discharging port (3), an internal and external circulation mixing unit (4), a circulation pump (5), a circulation port (6), a stirring shaft (7), and a driving component (8). At least three feeding ports (2) are provided above the mixing tank (1), and a discharging port (3) is provided at its lower end. The internal and external circulation mixing unit (4) is installed on the mixing tank (1), and the internal and external circulation mixing unit (4) is connected to the corresponding circulation ports (6) on the mixing tank (1) via four circulation pumps (5). The upper end of the stirring shaft (7), which is rotatably installed inside the mixing tank (1), is fixed to the output end of the driving component (8). The driving component (8) is fixed... The mixing vessel (1) is installed above the outside of the mixing vessel body (1). The internal and external circulation mixing (4) includes a built-in injection channel (41), injection head (42), external pipe (43), external mixing pipe (44), steam heating box (45) and steam port (46). Several injection heads (42) are arranged in a circular array below the built-in injection channel (41). The built-in injection channel (41) is connected to the corresponding external mixing pipe (44) through two external pipes (43). The external pipes (43) are fixedly passed through the mixing vessel body (1). The steam heating box (45) fixedly installed on the external mixing pipe (44) is connected to the high-temperature steam generator through the pipe and the steam port (46).

2. The polypeptide protein mixing device as described in claim 1, characterized in that: The built-in injection channel (41) is located inside the mixing vessel (1) and above the stirring shaft (7). The built-in injection channel (41) is an annular pipe with its center overlapping the axis of the stirring shaft (7). Several injection heads (42) are arranged at an angle below the built-in injection channel (41) with their injection outlets facing the stirring shaft (7).

3. The polypeptide protein mixing device as described in claim 2, characterized in that: The built-in injection channel (41) is connected to the upper vertical part of the two inverted "Y"-shaped external mixing pipes (44) through two external pipes (43). The external mixing pipes (44) are set outside the mixing vessel (1). The two branches below the external mixing pipes (44) are connected to the corresponding circulation ports (6) set on the mixing vessel (1) through the circulation pump (5).

4. The polypeptide protein mixing device as described in claim 3, characterized in that: Each of the circulation ports (6) in the mixing vessel (1) is equipped with a filter screen. The circulation pump (5) can draw the raw material from the bottom of the mixing vessel (1) into the external mixing pipe (44), and then flow through the external pipe (43) into the built-in injection channel (41), and then spray it onto the surface above the stirring shaft (7) through a number of injection heads (42) in the built-in injection channel (41).

5. The polypeptide protein mixing device as described in claim 4, characterized in that: A steam heating box (45) is fixedly installed on the outside of the vertical part of the external mixing pipe (44), and the two steam ports (46) provided on the steam heating box (45) are the discharge port and the injection port, respectively.

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