A 48-well deep well plate suitable for use in an automated pipetting workstation

Abstract

The utility model belongs to the technical field of deep hole plate, especially 48 hole deep hole plate suitable for automatic pipetting workstation, including the plate body and the multiple columns deep hole of arrangement on the plate body, the lower gap between two adjacent columns deep hole is the space of bottom magnetic attraction position, the bottom magnetic attraction position height of two deep holes of multiple columns deep hole side by side corresponding is 5% 20% of deep hole height, is equipped with the spoiler in deep hole. The utility model increases the height of bottom magnetic attraction position, increases adsorption area, strengthens adsorption force in deep hole side portion, shortens adsorption time to magnetic bead, and avoids multilayer stacking of magnetic bead, improves adsorption efficiency, in addition, the spoiler also promotes the flow and mixing of reagent or sample in centrifugal tube, and mixing efficiency is improved significantly, reduces mixing time, improves experimental efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of deep hole plate technology, and in particular a 48-well deep hole plate suitable for automated pipetting workstations. Background Technology

[0002] Automated pipetting workstations are widely used in scientific research, clinical testing, and drug development to improve experimental efficiency and reduce human error. 48-well deep-well plates, as a common laboratory consumable, are frequently used for storing and processing samples and reagents. However, existing 48-well deep-well plates have some shortcomings in their use.

[0003] Existing deep-well plates have a magnetic adsorption depth of only 1-2 mm at the bottom and a magnetic adsorption liquid level of only 200-300 μL. The adsorption area is small, the adsorption effect is poor, and it takes a long time to complete the adsorption of magnetic beads. Alternatively, due to the small adsorption surface, the magnetic beads may stack and adsorb, resulting in a large degree of mutual interference. They are not suitable for large-volume pipetting operations.

[0004] Another existing deep-hole plate, matched with a bottom magnetic suction frame, attracts magnetic beads at the bottom of the deep hole. Although the adsorption area is large, the magnetic beads at the bottom are easily attracted when the supernatant is extracted later, making it unsuitable for automated pipetting.

[0005] In addition, when mixing reagents or samples, the lack of effective auxiliary mixing structures in the wells often requires extending the mixing time or increasing the mixing amplitude, which is cumbersome and results in unstable mixing effects.

[0006] These issues, to some extent, affected the convenience and efficiency of the experiment. Utility Model Content

[0007] The purpose of this invention is to solve the problems of small bottom magnetic attraction height, poor adsorption effect, and poor turbulence effect in existing deep-hole plates, and to propose a 48-well deep-hole plate suitable for automated pipetting workstations.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: This utility model discloses a 48-well deep-hole plate suitable for automated pipetting workstations, comprising a plate body and multiple rows of deep holes arranged on the plate body. The lower gap between two adjacent rows of deep holes serves as the space for bottom magnetic attraction positions. The height of the bottom magnetic attraction positions of two corresponding deep holes side by side in the multiple rows of deep holes is 5%-20% of the depth of the deep hole. A flow-disrupting element is installed inside the deep hole.

[0009] As a further preferred option, the height of the bottom magnetic attachment point is 4-10mm.

[0010] As a further preferred option, the bottom of the deep hole has two inclined surfaces on both sides, and the space outside the inclined surfaces is the bottom magnetic attraction position. The angle between the inclined surfaces and the bottom of the deep hole is 5°-20°.

[0011] As a further preferred option, the bottom two sides of the deep hole are curved surfaces, and the space outside the curved surfaces is the bottom magnetic attraction position, with the radius of curvature of the curved surfaces being 5-15mm.

[0012] As a further preferred option, the depth of the deep hole is 35mm.

[0013] As a further preferred embodiment, the number of spoilers is two, with the two spoilers located on two different inclined surfaces.

[0014] As a further preferred embodiment, the number of spoilers is two, and the two spoilers are located on two arc surfaces respectively.

[0015] As a further preferred option, the spoiler is located in a cylindrical structure.

[0016] As a further preferred option, the spoiler is parallel to the length direction of the multiple rows of deep holes.

[0017] As a further preferred option, the diameter of the spoiler is 1.0-2.5mm and the height is 5-20mm.

[0018] This invention increases the height of the bottom magnetic attraction position, increases the adsorption area, strengthens the adsorption force on the side of the deep hole, shortens the adsorption time of the magnetic beads, and avoids the multi-layer stacking of magnetic beads, thus improving the adsorption efficiency. In addition, the turbulence element also promotes the flow and mixing of reagents or samples in the centrifuge tube, significantly improving the mixing efficiency, reducing the mixing time, and improving the experimental efficiency. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the internal structure of an existing deep hole plate. Figure 2 This is a schematic diagram of the internal structure of the 48-hole deep hole plate of this utility model; Figure 3 This is a schematic diagram of the bottom structure of the 48-hole deep hole plate of this utility model; Figure 4 This is a top view of the 48-hole deep hole plate of this utility model; In the diagram: 1. Plate body, 2. Deep hole, 3. Bottom magnetic suction position, 4. Baffle. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] In a traditional 48-hole deep hole plate, the deep hole 2 has a flat structure. The space between the two sides of the flat structure, that is, the connection side with the adjacent deep hole 2, is the bottom magnetic attraction position 3, which is used to place magnetic attractants. The magnetic attractants at this position play a role in attracting magnetic beads in the deep hole 2, or the magnetic attractants can be arranged at the bottom of the deep hole 2.

[0022] As an embodiment of this patent, when the bottom magnetic suction position 3 is arranged on the side of the deep hole 2, its height range on the side, i.e., the size of the entire adsorption surface, directly affects the adsorption effect on the magnetic beads in the deep hole 2. In the prior art, the height of the bottom magnetic suction position 3 is limited, that is, the entire adsorption area is limited. It is necessary to shake and for a long time to completely adsorb the magnetic beads in the deep hole 2 onto the adsorption surface, and there is stacking. In this embodiment, the following method is used to solve this problem: the height of the bottom magnetic suction position 3 is increased. The height of the bottom magnetic suction position 3 is designed to be 5%-20% of the height of the deep hole 2, which translates to an actual height of 4-10mm. Compared with the height of the prior art, at this height, the space of the bottom magnetic suction position 3 is increased, which allows the magnetic frame to be embedded in the bottom magnetic suction position 3, increasing the magnetic area and enhancing the magnetic effect. The 48-well deep-well plate can achieve a magnetic liquid level of 1000-1100μL, accommodating a larger volume of magnetically adsorbable liquid. It is also matched with a side magnetic adsorption frame, which makes it easier to transfer the supernatant after magnetic adsorption and avoids the problem of incomplete separation caused by the accumulation of magnetic beads at the bottom. When matched with an automated pipetting workstation, it can improve work efficiency.

[0023] As an embodiment of this patent, the bottom two sides (both sides of the flat structure) of the deep hole 2 are inclined surfaces, and the space outside the inclined surfaces is the bottom magnetic suction position 3. The angle between the inclined surfaces and the bottom of the deep hole 2 is 5°-20°, that is, the bottom magnetic suction position 3 is a triangular structure, and the height of the triangle is the height at which the magnetic rack can be placed.

[0024] As an embodiment of this patent, the bottom two sides of the deep hole 2 (the two sides of the flat structure) are arc surfaces, and the space outside the arc surfaces is the bottom magnetic suction position 3. The radius of curvature of the arc surfaces is 5-15mm, that is, the bottom magnetic suction position 3 is a triangular structure (the two sides of the triangle are concave), and the height of the triangular structure is the height at which the magnetic rack can be placed.

[0025] In both of the above embodiments, whether it is the inclined surface structure design or the arc surface structure design, both can ensure that the reagent or sample inside the deep hole 2 can flow smoothly at that position during oscillation, without dead corners that would affect the mixing effect. The difference is that the triangular structure has a significant liquid impact effect during oscillation, which is suitable for reagents or samples with higher viscosity, while the arc surface structure is smoother and the liquid level will be higher due to liquid inertia during oscillation, which is more suitable for experiments with a small amount of supernatant.

[0026] As one embodiment of this patent, the sample processing volume within each well is increased. The maximum capacity of a single well 2 in the original deep-well plate is 4.6 mL. In this patent, the height of well 2 is increased to 40 mm, and the maximum capacity of well 2 can reach 7 mL, significantly increasing the reaction volume of liquid reagents in each well of the deep-well plate. When matched with an automated pipetting workstation, it can accommodate larger sample processing volumes.

[0027] To verify the differences in magnetic attraction effects based on the height, slope angle, and radius of curvature of the bottom magnetic attraction point 3, 2 mL of hydroxyl magnetic beads (200 mg / mL) were used to test the magnetic attraction effect without the presence of a flow deflector 4. 2 mL of hydroxyl magnetic beads (200 mg / mL) were added to the wells of a standard 48-well plate and optimized 48-well plates of various sizes. The plates were vortexed at 1000 rpm for 3 minutes and then placed on the corresponding magnetic racks for magnetic attraction. The magnetic attraction time (from placement on the magnetic rack to complete adsorption of the magnetic beads) and the adsorption efficiency (the ratio of the magnetic bead stack thickness to the left-right dispersion distance) were recorded. Shorter magnetic attraction time and higher adsorption efficiency indicate better magnetic attraction. The results are as follows:

[0028] Based on the above results, it can be concluded that increasing the height of the bottom magnetic attraction position 3 can significantly improve the adsorption effect, and the adsorption effect of the optimized 48-well plate is significantly better than that of the ordinary 48-well plate.

[0029] As one embodiment of this patent, a flow-dispersing element 4 is provided inside the deep well 2. The flow-dispersing element 4 is not limited to any location within the deep well 2; it can be installed at the bottom or side, and its number and shape are also unrestricted. The flow-dispersing element 4 effectively promotes the flow and mixing of reagents or samples within the centrifuge tube. The columnar protrusions inside the deep well act as a stirrer when mixing reagents or samples, increasing liquid flow and collision, allowing for more thorough mixing of samples and reagents. Compared to traditional deep-well plates, mixing efficiency is significantly improved, mixing time is reduced, and experimental efficiency is increased. For example, during the extraction of E. coli plasmids, the speed and efficiency of cell lysis and neutralization can be accelerated by the side flow-dispersing element.

[0030] Furthermore, there are two flow-dispersing elements 4, which are symmetrically arranged on the left and right sides inside the hole. The two flow-dispersing elements 4 are located on two inclined surfaces, which enhance the mixing effect when the liquid impacts inside the deep hole 2.

[0031] Furthermore, there are two turbulence-disrupting elements 4, which are symmetrically arranged on the left and right sides inside the hole. The two turbulence-disrupting elements 4 are located on two arc surfaces respectively, which enhance the mixing effect under the inertia of liquid oscillation.

[0032] Furthermore, the spoiler 4 can be a cylindrical structure with a diameter of 1.0-2.5mm and a height of 10-20mm. The spoiler 4 can also be rectangular, conical, or other irregular shapes. As an embodiment of this patent, the orientation of the baffle 4 can be horizontal, oblique, or vertical. In this embodiment, the baffle 4 is preferably arranged vertically, that is, parallel to the length direction of the multiple rows of deep holes 2.

[0033] It should be noted that in the 48-hole deep hole plate of this utility model, the deep holes 2 on both sides of the plate do not have an adjacent deep hole 2, that is, the bottom magnetic attraction position 3 is also on the side of the deep hole 2.

[0034] To verify the differences in mixing effect between different sizes of the turbulence-disrupting component 4, a mixing test of a viscous liquid was conducted using 10 mL of glycerol, 20 mL of pure water, and 50 μL of 1% bromophenol blue solution, with the bottom magnetic suction position 3 present (structural parameter number 2 selected). The three reagents were added sequentially to wells of different sizes from the above examples, and the plates were shaken at 1200 rpm for 1 min 30 s. The upper, middle, and lower portions of the liquid in the centrifuge tubes were then taken, and the absorbance at 595 nm was measured using a microplate reader. The CV value was calculated. A smaller CV value indicates a better mixing effect. The results are as follows:

[0035] Based on the above results, the CV values ​​of items 2-10 are not significantly different and are relatively small, indicating a relatively good mixing effect. Therefore, the optimal size of the bottom spoiler 5 is a diameter of 1.0-2.5 mm and a height of 5-20 mm.

[0036] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A 48-well deep-hole plate suitable for automated pipetting workstations, comprising a plate body (1) and multiple rows of deep holes (2) arranged on the plate body (1), wherein the lower gap between two adjacent rows of deep holes (2) is the space for bottom magnetic attraction positions (3), characterized in that: The height of the bottom magnetic attraction position (3) of two corresponding deep holes (2) in the multi-row deep holes (2) is 5%-20% of the height of the deep hole (2); A flow-disrupting element (4) is provided inside the deep hole (2).

2. The 48-well deep-hole plate suitable for automated pipetting workstations according to claim 1, characterized in that: The bottom magnetic attraction position (3) has a height of 4-10mm.

3. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 2, characterized in that: The bottom two sides of the deep hole (2) are inclined surfaces, and the space outside the inclined surfaces is the bottom magnetic attraction position (3). The angle between the inclined surfaces and the bottom of the deep hole (2) is 5°-20°.

4. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 2, characterized in that: The bottom two sides of the deep hole (2) are arc surfaces, and the space outside the arc surfaces is the bottom magnetic suction position (3), and the radius of curvature of the arc surfaces is 5-15mm.

5. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 3 or 4, characterized in that: The height of the deep hole (2) is 40 mm.

6. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 3, characterized in that: The number of the baffles (4) is two, and the two baffles (4) are located on two inclined surfaces respectively.

7. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 4, characterized in that: The number of the two baffles (4) is two, and the two baffles (4) are located on two arc surfaces respectively.

8. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 6 or 7, characterized in that: The spoiler (4) is located in the cylindrical structure.

9. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 6 or 7, characterized in that: The turbulence element (4) is parallel to the length direction of the multiple rows of deep holes (2).

10. A 48-well deep-hole plate suitable for automated pipetting workstations according to claim 6 or 7, characterized in that: The spoiler (4) has a diameter of 1.0-2.5 mm and a height of 5-20 mm.

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