Quantitative cell culture medium dispensing device
Patent Information
- Application Number
- CN202522363902.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-07
- Publication Date
- 2026-10-09
- Estimated Expiration
- 2035-11-07
AI Technical Summary
[0003]然而，为实现对每一个培养皿内培养基的分装，定量分装仪在将培养基排出时，需要使用到转盘对每一个培养皿的转动，从而达到对每一个培养皿内培养基的分装，由于为便于将培养皿放入到转盘的内部，培养皿与转盘的放置槽之间存在一定的间隙，转盘的转动则会带动培养皿的转动，此时，转盘便会与放置槽的内壁存在一定的碰撞，随着碰撞次数的增加，极易造成培养皿的损坏，从而影响到培养皿的正常分装
本实用新型通过培养皿本体、放置板、限位板、伸缩杆和第一弹簧卡件之间的配合，利用限位板的设置，实现了对培养皿本体在放置板内放置位置的限制作用，采用改变第一弹簧卡件与其中一个通孔的连接方式，使得限位板在放置板内的位置可根据培养皿本体的实际尺寸进行调节，从而加固培养皿本体在放置板内的稳定性，还能防止培养皿本体与放置板的碰撞。
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Figure CN224830211U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, specifically a quantitative cell culture medium dispensing device. Background Technology
[0002] Cell culture refers to a method of simulating the in vivo environment with sterility, suitable temperature, pH, and certain nutritional conditions to enable organisms to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Cell culture is an indispensable process for both the entire bioengineering technology and biological cloning technology. Cell culture itself is the large-scale cloning of cells. Cell culture technology can cultivate a single cell into a large number of simple single cells or very little differentiated multicellular cells. This is an essential link in cloning technology. Moreover, cell culture itself is cell cloning. Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell synthesis and metabolism, cell growth and proliferation can be studied. In cell culture experiments, preparing culture medium is one of the most basic and important operations. To facilitate quantitative and uniform dispensing of culture medium into the culture dish, a quantitative dispensing instrument is used to dispense the culture medium.
[0003] However, in order to dispense the culture medium into each petri dish, the quantitative dispensing instrument needs to use a turntable to rotate each petri dish when discharging the culture medium. Because there is a certain gap between the petri dish and the placement slot of the turntable to facilitate the placement of the petri dish into the turntable, the rotation of the turntable will drive the rotation of the petri dish. At this time, the turntable will collide with the inner wall of the placement slot. With the increase of the number of collisions, the petri dish is easily damaged, thus affecting the normal dispensing of the petri dish. Utility Model Content
[0004] To address the shortcomings of existing technologies, this invention provides a quantitative cell culture medium dispensing device, which has the advantages of enhancing the stability of culture dishes placed in a turntable, facilitating the removal of multiple culture dishes from the turntable, and saving time in removing multiple culture dishes from the turntable, thus solving the problems mentioned in the background art.
[0005] This utility model provides the following technical solution: a quantitative cell culture medium dispensing device, including a storage tank body, a turntable is provided on the left side of the storage tank body, a plurality of slots are evenly opened on the upper end surface of the turntable, a placement plate is movably installed inside each slot, a culture dish body is provided inside the placement plate, a limiting plate is provided inside the placement plate on one side of the culture dish body, a gasket is fixedly installed on the outer side of the limiting plate, a telescopic rod is fixedly connected to the inner side of the limiting plate, a crossbar located outside the limiting plate is movably sleeved on the outer wall of the telescopic rod, and the outer end of the crossbar is fixedly connected to the outer wall of the placement plate, a first spring clip for connecting the crossbar is provided on the inner wall of the upper end surface of the telescopic rod, and the upper end of the first spring clip extends to the top of the crossbar, a sleeve rod is fixedly installed on the inner end of the crossbar, and pull rods are fixedly connected to both the front and rear ends of the sleeve rod.
[0006] Preferably, a locking block is fixedly installed on the lower end face of the turntable, and an installation rod is movably engaged on the inner wall of the locking block at the end away from the turntable. The front and back of the installation rod are each provided with a second spring clip for connecting the locking block. A rotating assembly for controlling the rotation of the turntable is fixedly installed on the lower end of the installation rod. A base located outside the rotating assembly is movably sleeved on the outer wall of the lower end of the installation rod. Several grooves located on the outer wall of the locking slots are opened on the upper end face of the turntable. A protrusion is movably engaged on the inner wall of the groove, and the inner end of the protrusion is fixedly connected to the outer wall of the placement plate. A threaded rod for connecting a crossbar is fixedly installed on the upper end face of the turntable, and a knob is threadedly connected to the outer wall of the threaded rod.
[0007] Preferably, a support plate is fixedly installed at the inner end of the crossbar, and the upper end face of the support plate is fixedly connected to the lower end of the sleeve rod. The inner wall of the support plate is movably sleeved with the outer wall of the threaded rod. A plurality of through holes for connecting the first spring clip are evenly opened on the upper end face of the crossbar. A spring is fixedly connected to the inner wall of the crossbar, and the end of the spring away from the crossbar is fixedly connected to the inner end of the telescopic rod.
[0008] Preferably, the rotating assembly includes a driven gear and a driving gear, wherein the front end of the driven gear and the rear end of the driving gear are engaged by toothed meshing.
[0009] Preferably, a column is fixedly sleeved on the inner wall of the driven gear, and the upper end of the column is fixedly connected to the lower end of the mounting rod. A baffle located below the rotating assembly is fixedly sleeved on the outer wall of the column. A plurality of second ball bearings are rotatably connected to the lower end face of the baffle. A first ball bearing is rotatably connected to the lower end of the column. A cylindrical rod is movably inserted into the outer wall of the column, and the lower end of the cylindrical rod is fixedly connected to the bottom surface of the inner wall of the base. The lower end of the baffle is rotatably connected to the upper end of the cylindrical rod through the second ball bearings. The lower end of the column is rotatably connected to the inner wall of the cylindrical rod through the first ball bearings.
[0010] Preferably, a rotating shaft is fixedly sleeved on the inner wall of the drive gear, and the upper end of the rotating shaft extends to the top of the base. A rotary motor is fixedly installed on the lower end of the rotating shaft. A surrounding plate is fixedly sleeved on the outer wall of the rotary motor, and the lower end face of the surrounding plate is fixedly connected to the bottom surface of the inner wall of the base.
[0011] Preferably, the outer wall of the knob has a number of anti-slip textures evenly distributed, and the inner wall of the knob is threadedly connected to the outer wall of the threaded rod.
[0012] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes the cooperation between the petri dish body, the placement plate, the limiting plate, the telescopic rod, and the first spring clip. By setting the limiting plate, the placement position of the petri dish body within the placement plate is restricted. By changing the connection method between the first spring clip and one of the through holes, the position of the limiting plate within the placement plate can be adjusted according to the actual size of the petri dish body, thereby strengthening the stability of the petri dish body within the placement plate and preventing collisions between the petri dish body and the placement plate.
[0013] This invention utilizes the cooperation between the storage tank body, turntable, culture dish body, placement plate, and pull rod. By using the pull rod to separate the placement plate from the turntable through the connection of the support plate, multiple culture dish bodies can be removed from the turntable simultaneously, saving the time of staff replacing each culture dish body one by one, thereby improving the dispensing efficiency of cell culture medium. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the turntable of this utility model; Figure 3 This is a schematic diagram of the knob of this utility model; Figure 4 This is a cross-sectional view of the cylindrical rod of this utility model; Figure 5 This is a schematic diagram of the rotating component of this utility model; Figure 6 This is a schematic diagram of the internal structure of the second spring clip of this utility model; Figure 7 This is a schematic diagram of the internal structure of the crossbar of this utility model; Figure 8 This utility model Figure 7 Enlarged view of point A; Figure 9 This is a schematic diagram of the card slot of this utility model.
[0015] In the diagram: 1. Storage tank body; 2. Turntable; 3. Slot; 4. Petri dish body; 5. Placement plate; 6. Limiting plate; 7. Gasket; 8. Telescopic rod; 9. Crossbar; 10. First spring clip; 11. Sleeve rod; 12. Pull rod; 13. Locking block; 14. Mounting rod; 15. Second spring clip; 16. Rotating assembly; 161. Driven gear; 162. Driven gear; 17. Base; 18. Groove; 19. Threaded rod; 20. Knob; 21. Anti-slip texture; 22. Support plate; 23. Protrusion; 24. Through hole; 25. Spring; 26. First ball bearing; 27. Column; 28. Baffle; 29. Second ball bearing; 30. Columnar rod; 31. Rotating shaft; 32. Rotary motor; 33. Enclosure. Detailed Implementation
[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0017] Please see Figure 1 , Figure 3 and Figure 4A quantitative cell culture medium dispensing device includes a storage tank body 1. A turntable 2 is arranged on the left side of the storage tank body 1. The rotation of the turntable 2 provides power for the rotation of a culture dish body 4, thereby switching between different culture dish bodies 4. Several slots 3 are evenly opened on the upper end surface of the turntable 2. The slots 3 provide space for a placement plate 5 to be placed on the turntable 2. A placement plate 5 is movably installed inside each slot 3. The placement plate 5 provides space for the placement of the culture dish body 4. The culture dish body 4 is placed inside the placement plate 5. A limiting plate 6 is set inside the placement plate 5 on one side of the culture dish body 4. The limiting plate 6 restricts the placement position of the culture dish body 4 within the placement plate 5. By changing the connection method between the first spring clip 10 and one of the through holes 24, the position of the limiting plate 6 within the placement plate 5 can be adjusted according to the actual size of the culture dish body 4, thereby strengthening the stability of the culture dish body 4 within the placement plate 5. A gasket 7 is fixedly installed on the outer side of the limiting plate 6. The gasket 7 increases the friction between the limiting plate 6 and the culture dish body 4. A telescopic rod 8 is fixedly connected to the inner side of the limiting plate 6. The compression and rebound of the telescopic rod 7 are used to adjust the position of the limiting plate 6 within the placement plate 5. A crossbar 9 located on the outer side of the limiting plate 6 is movably sleeved on the outer wall of the telescopic rod 8, and the outer end of the crossbar 9 is fixedly connected to the outer wall of the placement plate 5. A first spring clip 10 for connecting the crossbar 9 is provided on the inner wall of the upper end face of the telescopic rod 8, and the upper end of the first spring clip 10 extends above the crossbar 9. A sleeve rod 11 is fixedly installed on the inner end of the crossbar 9. Pull rods 12 are fixedly connected to both the front and rear ends of the sleeve rod 11. The placement plate 5 is separated from the turntable 2 by the pull rod 12 through the connection of the support plate 22, so that multiple culture dish bodies 4 can be removed from the turntable 2 at the same time, saving the time of changing the culture dish bodies 4 one by one by the staff, thereby improving the dispensing efficiency of cell culture medium.
[0018] A locking block 13 is fixedly installed on the lower end face of the turntable 2. An installation rod 14 is movably engaged with the inner wall of the locking block 13 at the end away from the turntable 2. The front and back of the installation rod 14 are each provided with a second spring catch 15 for connecting the locking block 13. A rotating assembly 16 for controlling the rotation of the turntable 2 is fixedly installed at the lower end of the installation rod 14. A base 17 located outside the rotating assembly 16 is movably sleeved on the outer wall of the lower end of the installation rod 14. Several slots 3 are located on the outer wall of the upper end face of the turntable 2. The inner wall of the groove 18 is movably engaged with a protrusion 23, and the inner end of the protrusion 23 is fixedly connected to the outer wall of the placement plate 5. A threaded rod 19 for connecting the crossbar 9 is fixedly installed on the upper end face of the turntable 2. A knob 20 is threadedly connected to the outer wall of the threaded rod 19. By using the threaded connection between the inner wall of the knob 20 and the outer wall of the threaded rod 19, the sleeve 11 is fitted onto the outside of the threaded rod 19, and the knob 20 is rotated and installed on the outside of the threaded rod 19, thereby achieving the connection between the turntable 2 and the placement plate 5.
[0019] Please see Figure 2 , Figure 5 and Figure 7 A support plate 22 is fixedly installed on the inner end of the crossbar 9, and the upper end face of the support plate 22 is fixedly connected to the lower end of the sleeve rod 11. The inner wall of the support plate 22 is movably sleeved with the outer wall of the threaded rod 19. Several through holes 24 for connecting the first spring clip 10 are evenly opened on the upper end face of the crossbar 9. A spring 25 is fixedly connected to the inner wall of the crossbar 9, and the end of the spring 25 away from the crossbar 9 is fixedly connected to the inner end of the telescopic rod 8. By compressing or rebounding the spring 25, the first spring clip 10 is connected to one of the through holes 24, thereby achieving the positioning of the limiting plate 6 after adjusting its position in the placement plate 5, so that the limiting plate 6 limits the culture dish body 4 in the placement plate 5.
[0020] The rotating assembly 16 includes a driven gear 161 and a driving gear 162, with the front end of the driven gear 161 meshing with the rear end of the driving gear 162.
[0021] Please see Figure 6 , Figure 8 and Figure 9 A column 27 is fixedly sleeved on the inner wall of the driven gear 161, and the upper end of the column 27 is fixedly connected to the lower end of the mounting rod 14. A baffle 28 located below the rotating assembly 16 is fixedly sleeved on the outer wall of the column 27. Several second balls 29 are rotatably connected to the lower end face of the baffle 28. A first ball 26 is rotatably connected to the lower end of the column 27. A cylindrical rod 30 is movably inserted into the outer wall of the column 27, and the lower end of the cylindrical rod 30 is fixedly connected to the bottom surface of the inner wall of the base 17. The lower end of the baffle 28 is rotatably connected to the upper end of the cylindrical rod 30 through the second balls 29. The lower end of the column 27 is rotatably connected to the inner wall of the cylindrical rod 30 through the first ball 26. At this time, the rotation of the column 27 drives the second balls 29 on the baffle 28 to rotate at the upper end of the cylindrical rod 30, and the first ball 26 at the bottom of the column 27 rotates at the bottom of the inner wall of the cylindrical rod 30.
[0022] A rotating shaft 31 is fixedly sleeved on the inner wall of the drive gear 162, and the upper end of the rotating shaft 31 extends to the top of the base 17. A rotary motor 32 is fixedly installed on the lower end of the rotating shaft 31. The rotary motor 32 provides power for the rotation of the drive gear 162 through the connection of the rotating shaft 31. A surrounding plate 33 is fixedly sleeved on the outer wall of the rotary motor 32, and the lower end face of the surrounding plate 33 is fixedly connected to the bottom surface of the inner wall of the base 17.
[0023] The outer wall of the knob 20 is evenly distributed with several anti-slip textures 21. The anti-slip textures 21 increase the friction between the operator's hand and the contact surface of the outer wall of the knob 20. The inner wall of the knob 20 is threadedly connected to the outer wall of the threaded rod 19. The threaded connection installation method not only makes it easy to install the knob 20 on the threaded rod 19, at which time the placement plate 5 can be fixed on the turntable 2, but also makes it easy to remove the knob 20 from the threaded rod 19. At this time, multiple placement plates 5 can be removed from the turntable 2 as a whole.
[0024] Working principle: In use, firstly, the culture dish body 4 containing the culture medium to be dispensed is placed inside the placement plate 5. To reinforce the tightness of the culture dish body 4 within the placement plate 5, the first spring clip 10 is pressed into the telescopic rod 8. At this time, after the first spring clip 10 separates from the crossbar 9, the compression or rebound of the spring 25 connects the first spring clip 10 with one of the through holes 24, achieving the positioning of the limiting plate 6 within the placement plate 5 after adjustment. This ensures that the limiting plate 6 is positioned within the placement plate 5 of the culture dish body. 4. Limit the position, then use the pull rod 12 to put the sleeve 11 on the outside of the threaded rod 19. Utilize the threaded connection between the inner wall of the knob 20 and the outer wall of the threaded rod 19, so that after the sleeve 11 is put on the outside of the threaded rod 19, rotate the knob 20 to install it on the outside of the threaded rod 19, achieving the connection between the turntable 2 and the placement plate 5. At this time, the groove 18 and the protrusion 23 are engaged. Then, turn on the power of the rotary motor 32. After the rotary motor 32 is turned on, it drives the rotation of the drive gear 162 through the connection of the rotating shaft 31. Since the drive gear 162... The connection between the drive gear 162 and the driven gear 161, with their teeth meshing, allows the drive gear 162 to rotate while simultaneously driving the driven gear 161. The rotation of the driven gear 161, in turn, drives the mounting rod 14 via the column 27. The movement of the mounting rod 14, in turn, drives the locking block 13 via the second spring clip 15. The rotation of the locking block 13 then drives the rotation of the turntable 2. Simultaneously, the rotation of the turntable 2 allows for the switching of the culture dish body 4 within the placement plate 5. At this time, the column 27 rotates while simultaneously driving the driven gear 161. The second ball bearing 29 on the moving baffle 28 rotates at the upper end of the cylindrical rod 30, and the first ball bearing 26 at the bottom of the column 27 rotates at the bottom of the inner wall of the cylindrical rod 30. Finally, after the culture dishes in the culture dish body 4 are dispensed, the power of the rotary motor 32 is turned off, the knob 20 is removed from the rotating part of the threaded rod 19, and the sleeve rod 11, support plate 22, crossbar 9, telescopic rod 8 and placement plate 5 are pulled out from the inside of the turntable 2 by the pull rod 12, so as to facilitate the removal of multiple culture dish bodies 4 from the turntable 2 as a whole.
[0025] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Additionally, in the accompanying drawings of this utility model, the fill patterns are merely for distinguishing layers and do not constitute any other limitation.
[0026] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A quantitative cell culture medium dispensing device, comprising a storage tank body, characterized in that: A turntable is provided on the left side of the storage tank body. Several slots are evenly distributed on the upper surface of the turntable. A placement plate is movably installed inside each slot. A petri dish body is placed inside the placement plate. A limiting plate located on one side of the petri dish body is provided inside the placement plate. A gasket is fixedly installed on the outer side of the limiting plate. A telescopic rod is fixedly connected to the inner side of the limiting plate. A crossbar located outside the limiting plate is movably sleeved on the outer wall of the telescopic rod, and the outer end of the crossbar is fixedly connected to the outer wall of the placement plate. A first spring clip for connecting the crossbar is provided on the inner wall of the upper surface of the telescopic rod, and the upper end of the first spring clip extends above the crossbar. A sleeve rod is fixedly installed on the inner end of the crossbar, and pull rods are fixedly connected to both the front and rear ends of the sleeve rod.
2. The quantitative cell culture medium dispensing device according to claim 1, characterized in that: A locking block is fixedly installed on the lower end face of the turntable. An installation rod is movably engaged on the inner wall of the locking block at the end away from the turntable. A second spring clip for connecting the locking block is provided on both the front and back of the installation rod. A rotating component for controlling the rotation of the turntable is fixedly installed on the lower end of the installation rod. A base located outside the rotating component is movably sleeved on the outer wall of the lower end of the installation rod. Several grooves located on the outer wall of the slot are opened on the upper end face of the turntable. A protrusion is movably engaged on the inner wall of the groove, and the inner end of the protrusion is fixedly connected to the outer wall of the placement plate. A threaded rod for connecting the crossbar is fixedly installed on the upper end face of the turntable. A knob is threadedly connected to the outer wall of the threaded rod.
3. The quantitative cell culture medium dispensing device according to claim 2, characterized in that: A support plate is fixedly installed at the inner end of the crossbar, and the upper end face of the support plate is fixedly connected to the lower end of the sleeve rod. The inner wall of the support plate is movably sleeved with the outer wall of the threaded rod. Several through holes for connecting the first spring clip are evenly opened on the upper end face of the crossbar. A spring is fixedly connected to the inner wall of the crossbar, and the end of the spring away from the crossbar is fixedly connected to the inner end of the telescopic rod.
4. The quantitative cell culture medium dispensing device according to claim 2, characterized in that: The rotating assembly includes a driven gear and a driving gear, with the front end of the driven gear meshing with the rear end of the driving gear.
5. A quantitative cell culture medium dispensing device according to claim 4, characterized in that: A column is fixedly sleeved on the inner wall of the driven gear, and the upper end of the column is fixedly connected to the lower end of the mounting rod. A baffle located below the rotating assembly is fixedly sleeved on the outer wall of the column. Several second ball bearings are rotatably connected to the lower end face of the baffle. A first ball bearing is rotatably connected to the lower end of the column. A cylindrical rod is movably inserted into the outer wall of the column, and the lower end of the cylindrical rod is fixedly connected to the bottom surface of the inner wall of the base. The lower end of the baffle and the upper end of the cylindrical rod are rotatably connected through the second ball bearings. The lower end of the column and the inner wall of the cylindrical rod are rotatably connected through the first ball bearing.
6. A quantitative cell culture medium dispensing device according to claim 4, characterized in that: The inner wall of the drive gear is fixedly fitted with a rotating shaft, and the upper end of the rotating shaft extends to the top of the base. The lower end of the rotating shaft is fixedly installed with a rotary motor. The outer wall of the rotary motor is fixedly fitted with a surrounding plate, and the lower end face of the surrounding plate is fixedly connected to the bottom surface of the inner wall of the base.
7. A quantitative cell culture medium dispensing device according to claim 2, characterized in that: The outer wall of the knob has several anti-slip textures evenly distributed, and the inner wall of the knob is threadedly connected to the outer wall of the threaded rod.