Extracellular vesicle extraction injection device

Abstract

The utility model relates to cell exosome extraction injection technical field discloses a cell exosome extraction injection device, including lifting assembly, upper connection structure, lower support structure, support rod, pipette and extraction injection assembly, lifting assembly installs on the support rod, upper connection structure sets up in the outside of support rod, and lifting assembly drive end is connected with the upper end of upper connection structure and is fixed, drives the vertical up and down movement of upper connection structure, lower support structure is fixed in the lower part outside of support rod, and extraction injection assembly sets up between upper connection structure and lower support structure, and pipette is connected in the bottom of extraction injection assembly, and the vertical distance between upper connection structure and lower support structure is adjusted, and drives extraction injection assembly to realize the extraction injection of cell exosome through pipette. The utility model can realize the batch extraction and injection operation of cell exosome, improve the operation efficiency to cell exosome, reduce the labor intensity of experimental personnel.

Description

Technical Field

[0001] This utility model relates to the field of exosome extraction and injection technology, and in particular to an exosome extraction and injection device. Background Technology

[0002] Exosomes are extracellular vesicles with a diameter of 40-200 nm (or 30-150 nm) formed by the fusion of multiple vesicles with the cell membrane. Studies have shown that exosomes can carry specific genetic information and serve as carriers for intercellular communication. The isolation of exosomes is fundamental to their use, and isolation methods include differential ultracentrifugation, immunohistolysis, and polymerase chain precipitation.

[0003] Currently, the isolation and extraction of exosomes typically involves manual aspiration and transfer. This process requires multiple aspirations, mixing, and injections of the cell fluid, especially during batch culture and extraction, resulting in high labor intensity, time-consuming operations, and low efficiency for researchers. While some exosome extraction devices exist, such as the Chinese invention patent application CN202411756298.9 entitled "An Exosome Isolation and Extraction Device," which loads various types and concentrations of exosomes into multiple reagent tubes to improve the accuracy of data in experimental analysis, it primarily focuses on centrifugation during cell isolation and extraction, without addressing the transfer of exosomes. Utility Model Content

[0004] In order to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide an exosome extraction and injection device that can realize the batch extraction and injection of exosomes, improve the efficiency of exosome operation, and reduce the labor intensity of experimental personnel.

[0005] This utility model is achieved using the following technical solution: an exosome extraction and injection device, comprising a lifting assembly, an upper connecting structure, a lower supporting structure, a supporting rod, a pipette, and an extraction and injection assembly. The lifting assembly is mounted on the supporting rod, and the upper connecting structure is disposed on the outside of the supporting rod. The driving end of the lifting assembly is connected and fixed to the upper end of the upper connecting structure, driving the upper connecting structure to move vertically up and down. The lower supporting structure is fixed to the lower outside of the supporting rod, and the extraction and injection assembly is disposed between the upper connecting structure and the lower supporting structure. The pipette is connected to the bottom end of the extraction and injection assembly. By adjusting the vertical distance between the upper connecting structure and the lower supporting structure, the extraction and injection assembly is driven to extract and inject exosomes through the pipette.

[0006] Furthermore, the extraction injection assembly includes a push-pull rod, a sleeve, and a conduit. A plug is fixed to the front end of the push-pull rod, and the plug is tightly fitted to the inner wall of the sleeve. The bottom end of the sleeve is connected to the upper end of the pipette through the conduit.

[0007] Furthermore, the upper connecting structure includes a first mounting plate, a rod seat, and a front fixing plate. The first mounting plate is fixed to the front end of the front fixing plate by screws. Multiple rod seats are evenly spaced at the front end of the first mounting plate, and the top end of the push-pull rod is fixed in the rod seat.

[0008] Furthermore, the lower support structure includes a second clamping plate, a third clamping plate, a fourth clamping plate, a clamping plate connecting plate, and a clamping plate fixing plate. Two clamping plate fixing plates are symmetrically fixed on both sides of the rear end of the clamping plate connecting plate. The clamping plate fixing plates are fixed to the lower outer side of the support rod. The second, third, and fourth clamping plates are sequentially fixed to the front end of the clamping plate connecting plate from top to bottom by bolts. The upper and lower parts of the sleeve are respectively clamped in the second and third clamping plates, and the upper part of the pipette is clamped in the fourth clamping plate.

[0009] Furthermore, the lifting assembly includes a drive assembly, a mounting base, a lead screw bearing seat, a lead screw, and a lead screw nut. The two mounting bases are respectively fixed to the top and bottom of the support rod by screws. The lead screw bearing seat is fixed on the mounting base. The two ends of the lead screw are rotatably mounted in the two lead screw bearing seats. The lead screw nut is mounted on the lead screw. The front fixing plate is fixed on the lead screw nut. The drive assembly is connected and fixed to the lead screw, driving the lead screw to rotate.

[0010] Furthermore, the drive assembly includes a support column, a motor, and a coupling. The motor is fixed to the upper end of the mounting base located above by the support column, and the drive end of the motor is connected to the upper end of the lead screw by the coupling.

[0011] Furthermore, the support rod has a screw groove at the center of its front end for mounting a screw, and guide grooves are symmetrically arranged on the left and right sides of the support rod. Several guide wheels are rotatably and symmetrically installed at the rear end of the front fixed plate. The guide wheels are arranged in the guide grooves and roll vertically up and down along the guide grooves.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows: The extracellular morphology extraction and injection device of this utility model, in which the push-pull rod moves vertically up and down, drives the plug to slide inside the sleeve, so that the inside of the sleeve is vacuumed or pressurized, thereby aspirating or injecting extracellular morphology through the pipette, realizing the batch extraction and injection operation of extracellular morphology, improving the operation efficiency of extracellular morphology, and reducing the labor intensity of experimental personnel. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural schematic diagram of the extracellular oothecia extraction and injection device of this utility model;

[0014] Figure 2 This is a left view of the exosome extraction and injection device of this utility model;

[0015] Figure 3 This is a schematic diagram of the structure of this utility model after removing the lifting component and support rod;

[0016] Figure 4 This is a structural schematic diagram of the lifting component in this utility model.

[0017] In the diagram: Lifting assembly 1; Upper connecting structure 2; Lower support structure 3; Guide wheel 4; Support rod 5; Pipette 6; Extraction and injection assembly 7; Mounting base 12; Support column 13; Motor 14; Coupling 15; Lead screw bearing seat 16; Lead screw 17; Lead screw nut 18; First mounting plate 21; Rod seat 22; Second clamping plate 23; Third clamping plate 24; Fourth clamping plate 25; Clamping plate connecting plate 26; Clamping plate fixing plate 27; Front fixing plate 28; Push-pull rod 31; Sleeve 32; Guide tube 33; Guide groove 51; Lead screw groove 52. Detailed Implementation

[0018] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0019] The purpose of this invention is to address the shortcomings of existing technologies by providing an exosome extraction and injection device.

[0020] Example 1

[0021] This embodiment provides an exosome extraction and injection device, referring to... Figure 1As shown, the assembly includes a lifting component 1, an upper connecting structure 2, a lower supporting structure 3, a supporting rod 5, a pipette 6, and an extraction and injection assembly 7. The lifting component 1 is mounted on the supporting rod 5, and the upper connecting structure 2 is located on the outside of the supporting rod 5. The driving end of the lifting component 1 is connected and fixed to the upper end of the upper connecting structure 2, causing the upper connecting structure 2 to move vertically up and down on the outside of the supporting rod 5. The lower supporting structure 3 is fixed to the lower outer part of the supporting rod 5. The extraction and injection assembly 7 is located between the upper connecting structure 2 and the lower supporting structure 3. The pipette 6 is connected to the bottom end of the extraction and injection assembly 7. By adjusting the vertical distance between the upper connecting structure 2 and the lower supporting structure 3, the extraction and injection assembly 7 can achieve batch extraction and injection of extracellular oozosomes through the pipette 6.

[0022] Reference Figures 2-3 As shown, the extraction and injection assembly 7 includes a push-pull rod 31, a sleeve 32, and a conduit 33. A stopper is fixed to the front end of the push-pull rod 31, and the stopper fits tightly against the inner wall of the sleeve 32. The bottom end of the sleeve 32 is connected to the upper end of the pipette 6 via the conduit 33. As the push-pull rod 31 moves vertically up and down, it causes the stopper to slide inside the sleeve 32, creating a vacuum or pressure inside the sleeve 32, thereby allowing the pipette 6 to aspirate or inject exosomes. In this embodiment, the push-pull rod 31, sleeve 32, conduit 33, and pipette 6 are connected in a one-to-one correspondence, and there are multiple of each, enabling batch extraction and injection of exosomes, improving operational efficiency, and reducing the workload of laboratory personnel.

[0023] Reference Figures 2-3 As shown, the upper connecting structure 2 includes a first mounting plate 21, a rod seat 22 and a front fixing plate 28. The first mounting plate 21 is fixed to the front end of the front fixing plate 28 by screws. Multiple rod seats 22 are evenly spaced at the front end of the first mounting plate 21. The top end of the push-pull rod 31 is fixed in the rod seat 22.

[0024] Reference Figures 2-3 As shown, the lower support structure 3 includes a second clamping plate 23, a third clamping plate 24, a fourth clamping plate 25, a clamping plate connecting plate 26, and a clamping plate fixing plate 27. Two clamping plate fixing plates 27 are symmetrically fixed on both sides of the rear end of the clamping plate connecting plate 26. The clamping plate fixing plates 27 are fixed to the lower outer side of the support rod 5 by bolts. The second clamping plate 23, the third clamping plate 24, and the fourth clamping plate 25 are fixed to the front end of the clamping plate connecting plate 26 from top to bottom by bolts. The upper and lower parts of the sleeve 32 are respectively clamped in the second clamping plate 23 and the third clamping plate 24, and the upper part of the pipette 6 is clamped in the fourth clamping plate 25.

[0025] Reference Figure 4As shown, the lifting assembly 1 includes a drive assembly, a mounting base 12, a lead screw bearing seat 16, a lead screw 17, and a lead screw nut 18. Two mounting bases 12 are fixed to the top and bottom of the support rod 5 respectively with screws. The lead screw bearing seat 16 is fixed to the mounting base 12. The two ends of the lead screw 17 are rotatably mounted in the two lead screw bearing seats 16. The lead screw nut 18 is mounted on the lead screw 17. The front fixing plate 28 is fixed to the lead screw nut 18. The drive assembly is connected and fixed to the lead screw 17, driving the lead screw 17 to rotate. Specifically, the drive assembly includes a support column 13, a motor 14, and a coupling 15. The motor 14 is fixed to the upper end of the mounting base 12 located above via the support column 13. The drive end of the motor 14 is connected to the upper end of the lead screw 17 via the coupling 15.

[0026] Reference Figures 1-4 As shown, the support rod 5 has a screw groove 52 for mounting the screw 17 at the center of its front end. The support rod 5 has guide grooves 51 symmetrically arranged on its left and right sides. The front fixed plate 28 has four guide wheels 4 symmetrically mounted on its rear end. The guide wheels 4 are set in the guide grooves 51 and roll vertically up and down along the guide grooves 51.

[0027] This invention discloses an extracellular oothecia extraction and injection device, which can be used in conjunction with existing translational devices such as electric two-dimensional precision slides to drive the extracellular oothecia extraction and injection device in horizontal linear motion. During use, the extracellular oothecia extraction and injection device is driven by a motor 14 to rotate a lead screw 17. As the two ends of the lead screw 17 rotate within the lead screw bearing seat 16, the screw nut 18 moves vertically up and down through threaded engagement, thereby driving the front fixed plate 28 to move vertically up and down. During this movement, the front fixed plate 28 utilizes its rear guide wheel 4 to roll vertically up and down in the guide groove 51, maintaining the stability of the front fixed plate 28. During the vertical movement of the front fixed plate 28, the push-pull rod 31 moves vertically up and down, causing the plug at its bottom end to slide inside the sleeve 32. When the push-pull rod 31 moves upward, it evacuates the inside of the sleeve 32, thereby drawing exosomes from the external container through the pipette 6. When the push-pull rod 31 moves downward, it compresses the inside of the sleeve 32, thereby injecting exosomes into the outside through the pipette 6. This enables batch extraction and injection of exosomes, improving the efficiency of exosome handling and reducing the labor intensity of experimental personnel.

[0028] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An exosome extraction and injection device, characterized in that: The device includes a lifting assembly (1), an upper connecting structure (2), a lower supporting structure (3), a supporting rod (5), a pipette (6), and an extraction and injection assembly (7). The lifting assembly (1) is mounted on the supporting rod (5), and the upper connecting structure (2) is located on the outside of the supporting rod (5). The driving end of the lifting assembly (1) is connected and fixed to the upper end of the upper connecting structure (2), driving the upper connecting structure (2) to move vertically up and down. The lower supporting structure (3) is fixed on the lower outside of the supporting rod (5). The extraction and injection assembly (7) is located between the upper connecting structure (2) and the lower supporting structure (3). The pipette (6) is connected to the bottom end of the extraction and injection assembly (7). By adjusting the vertical distance between the upper connecting structure (2) and the lower supporting structure (3), the extraction and injection assembly (7) is driven to extract and inject extracellular oozosomes through the pipette (6).

2. The exosome extraction and injection device according to claim 1, characterized in that: The extraction injection assembly (7) includes a push-pull rod (31), a sleeve (32) and a conduit (33). A plug is fixed at the front end of the push-pull rod (31), and the plug fits tightly against the inner wall of the sleeve (32). The bottom end of the sleeve (32) is connected to the upper end of the pipette (6) through the conduit (33).

3. The exosome extraction and injection device according to claim 2, characterized in that: The upper connection structure (2) includes a first mounting plate (21), a rod seat (22) and a front fixing plate (28). The first mounting plate (21) is fixed to the front end of the front fixing plate (28) by screws. Multiple rod seats (22) are evenly spaced at the front end of the first mounting plate (21). The top end of the push-pull rod (31) is fixed in the rod seat (22).

4. The exosome extraction and injection device according to claim 3, characterized in that: The lower support structure (3) includes a second clamping plate (23), a third clamping plate (24), a fourth clamping plate (25), a clamping plate connecting plate (26), and a clamping plate fixing plate (27). Two clamping plate fixing plates (27) are symmetrically fixed on both sides of the rear end of the clamping plate connecting plate (26). The clamping plate fixing plates (27) are fixed on the lower outer side of the support rod (5). The second clamping plate (23), the third clamping plate (24), and the fourth clamping plate (25) are fixed to the front end of the clamping plate connecting plate (26) from top to bottom by bolts. The upper and lower parts of the sleeve (32) are respectively clamped in the second clamping plate (23) and the third clamping plate (24). The upper part of the pipette (6) is clamped in the fourth clamping plate (25).

5. The exosome extraction and injection device according to claim 4, characterized in that: The lifting assembly (1) includes a drive assembly, a mounting base (12), a lead screw bearing seat (16), a lead screw (17), and a lead screw nut (18). The two mounting bases (12) are fixed to the top and bottom of the support rod (5) respectively by screws. The lead screw bearing seat (16) is fixed on the mounting base (12). The two ends of the lead screw (17) are rotatably mounted in the two lead screw bearing seats (16). The lead screw nut (18) is mounted on the lead screw (17). The front fixing plate (28) is fixed on the lead screw nut (18). The drive assembly is connected and fixed to the lead screw (17) to drive the lead screw (17) to rotate.

6. The exosome extraction and injection device according to claim 5, characterized in that: The drive assembly includes a support column (13), a motor (14) and a coupling (15). The motor (14) is fixed to the upper end of the mounting base (12) located above by the support column (13). The drive end of the motor (14) is connected to the upper end of the lead screw (17) through the coupling (15).

7. The exosome extraction and injection device according to any one of claims 3 to 6, characterized in that: The support rod (5) has a screw groove (52) for mounting a screw (17) at the center of its front end. The support rod (5) has guide grooves (51) symmetrically arranged on its left and right sides. The front fixed plate (28) has several guide wheels (4) symmetrically mounted on its rear end. The guide wheels (4) are set in the guide grooves (51) and roll vertically up and down along the guide grooves (51).

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