A transfection plate for transient transfection of cells

Abstract

The utility model relates to the field of biotechnology especially a kind of transfection plate for cell instant transfection, to the problem that cell transfection plate in prior art is not convenient to adjust inclination angle and affects cell solution extraction in hole cavity, present and propose the following scheme, it includes well plate and the bottom plate of being located at well plate bottom surface, the top surface of well plate is equipped with multiple hole cavities, positive electrode and negative electrode are equipped in hole cavity, well plate bottom surface is inlaid with the power supply circuit for the intercommunication of positive electrode and negative electrode;The one end of the bottom plate is hinged with the one end of well plate, the other end of bottom plate is equipped with the adjusting mechanism for adjusting the inclination angle of well plate.The utility model greatly improves transfection efficiency by multi-hole synchronous electric stimulation design, ensures the consistency of cell transfection in the same hole, and the setting of adjusting mechanism can adjust the inclination angle of well plate, can make hole cavity tilt after transfection, cell solution converges to the low end of hole cavity due to gravity, thereby reducing residual rate.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to a transfection plate for instantaneous cell transfection. Background Technology

[0002] In the field of biotechnology, transient cell transfection is a key operational step in experiments such as gene function research and protein expression analysis. Its core principle is the efficient introduction of exogenous nucleic acids (such as DNA and RNA) into target cells to achieve short-term gene expression or regulation. Currently, multi-well transfection plates are the most commonly used cell transfection tools in laboratories. These plates provide independent reaction chambers, allowing for the simultaneous transfection of multiple samples, thus meeting the needs of high-throughput research.

[0003] However, existing transfection plates have relatively simple structures. On the one hand, although multi-electroporation transfection plates can perforate cell membranes through an electric field, the electrode layout is unreasonable, resulting in uneven electric field strength in different regions within the same well, leading to significant differences in cell transfection rates. Furthermore, simultaneous multi-well electrical stimulation cannot be achieved, resulting in prolonged experimental time. On the other hand, the tilt angle of existing transfection plates is inconvenient to adjust. After transfection, a pipette is needed to extract the cell solution from the well (for subsequent culture or detection). Since existing transfection plates are all horizontally fixed structures with flat or shallowly concave bottoms, cell solution easily adheres to the edges or corners of the well walls. Especially when the solution volume is small (e.g., below 20 μL), "dead volume" easily occurs during extraction, resulting in a high residual rate. This not only wastes samples but may also cause interference with subsequent experimental results due to residual transfection reagents in the solution. Therefore, this proposal suggests a transfection plate for instantaneous cell transfection. Utility Model Content

[0004] This invention proposes a transfection plate for instantaneous cell transfection, which solves the problem in the prior art where the tilt angle of the cell transfection plate is inconvenient to adjust, thus affecting the extraction of cell solution from the well.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A transfection plate for instantaneous cell transfection includes a well plate and a bottom plate disposed on the bottom surface of the well plate. The top surface of the well plate has multiple cavities, and positive and negative electrodes are disposed in the cavities. The bottom surface of the well plate is embedded with power supply lines for connecting the positive and negative electrodes.

[0007] One end of the base plate is hinged to one end of the perforated plate, and the other end of the base plate is equipped with an adjustment mechanism for adjusting the tilt angle of the perforated plate.

[0008] The adjustment mechanism includes an adjustment wheel rotatably connected to the end of the base plate via a rotating shaft, and an adjustment handle rotatably connected to the adjustment wheel at a position off-center on the circular surface away from the rotating shaft. The circular surface of the adjustment wheel is provided with a guide groove that is inclined from the inside to the outside. A fixing post is fixed to one end of the perforated plate near the adjustment wheel, and one end of the fixing post extends into the guide groove and slides with it.

[0009] A positioning component for maintaining the tilt angle of the orifice plate is also installed between the adjusting wheel and the base plate.

[0010] Through the above technical solutions, the multi-well synchronous electrical stimulation design significantly improves transfection efficiency, ensures the consistency of cell transfection within the same well, and the adjustment mechanism can adjust the tilt angle of the well plate, so that the well cavity is tilted after transfection, and the cell solution gathers to the lower end of the well cavity due to gravity, thereby reducing residue.

[0011] As a further improvement to the above solution, a cover plate is hinged to one side of the top surface of the perforated plate. The length and width of the cover plate are the same as those of the perforated plate. The side of the cover plate away from the hinge point with the perforated plate is fixed to the perforated plate by a latch.

[0012] As a further improvement to the above solution, the positioning component includes a rubber ball fixed on the circular surface of the adjusting wheel near the base plate and a plurality of locking holes opened at the end of the base plate for engaging the rubber ball, wherein the rubber ball is located off-center from the circular surface of the adjusting wheel.

[0013] As a further improvement to the above solution, the power supply line includes a positive electrode wire and a negative electrode wire arranged along the length direction of the orifice plate. Multiple positive electrode branches are connected to the positive electrode wire, and the positive electrode in the cavity is connected to the positive electrode branch. Multiple negative electrode branches are connected to the negative electrode wire, and the negative electrode in the cavity is connected to the negative electrode branch. The bottom surface of the orifice plate has a groove for fitting the positive electrode wire, the negative electrode wire, the positive electrode branch, and the negative electrode branch.

[0014] As a further improvement to the above solution, an electrical control box connected to the positive and negative wires is installed at one end of the orifice plate. A pulse power supply is installed inside the electrical control box, and a control knob for turning the pulse power supply on and off and an indicator light for displaying the working status are installed on the front of the electrical control box.

[0015] As a further improvement to the above solution, the tilt angle of the guide groove is set to 30-45 degrees.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] 1. By setting up power supply lines and an electrical control box, current pulse stimulation can be applied to the cell solutions in multiple wells simultaneously during transfection, thereby significantly accelerating the cell transfection speed.

[0018] 2. By using the base plate and setting the adjustment mechanism, after cell transfection, one end of the well plate can be raised at a certain angle, thereby tilting the well. Then, the cell solution in the well can be aspirated using a pipette or other aspiration tool, which can speed up the emptying of the cell solution in the well and reduce the residual cell solution in the well. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0021] Figure 3 This is an exploded view of the present invention;

[0022] Figure 4 This is a schematic diagram of the structure of a rubber ball;

[0023] Figure 5 This is a schematic diagram of the structure after the perforated plate is lifted at a certain angle.

[0024] Figure 6 This is a structural diagram of the electrical control box and the hinge.

[0025] Explanation of key symbols:

[0026] 1. Orifice plate; 2. Cover plate; 3. Orifice cavity; 4. Adjusting wheel; 5. Base plate; 6. Negative electrode; 7. Adjusting handle; 8. Guide groove; 9. Fixing post; 10. Positive electrode wire; 11. Positive electrode; 12. Positive electrode branch wire; 13. Negative electrode wire; 14. Negative electrode branch wire; 15. Locking hole; 16. Rotating shaft; 17. Electrical control box; 18. Rubber ball. Detailed Implementation

[0027] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0028] Example 1:

[0029] Please combine Figure 1 - Figure 6 This embodiment of a transfection plate for instantaneous cell transfection includes a well plate 1 and a base plate 5 disposed on the bottom surface of the well plate 1. The well plate 1 is made of transparent polystyrene.

[0030] The top surface of the perforated plate 1 has multiple cavities 3, and positive electrodes 11 and negative electrodes 6 are provided in the cavities 3. The bottom surface of the perforated plate 1 is embedded with power supply lines for connecting the positive electrodes 11 and negative electrodes 6. The power supply lines include positive conductors 10 and negative conductors 13 arranged along the length of the perforated plate 1. Multiple positive branch lines 12 arranged along the width of the perforated plate 1 are connected to the positive conductors 10. The positive electrodes 11 in each row of cavities 3 are connected to a corresponding positive branch line 12. Multiple negative branch lines 14 arranged along the width of the perforated plate 1 are connected to the negative conductors 13. The negative electrodes 6 in each row of cavities 3 are connected to a corresponding negative branch line 14, thereby realizing the series power supply of "main line - branch line - electrode" to ensure that the electric field strength in the same row of cavities 3 is consistent.

[0031] The bottom surface of the perforated plate 1 has grooves for fitting the positive electrode wire 10, the negative electrode wire 13, the positive electrode branch wire 12 and the negative electrode branch wire 14. The grooves are 0.5 mm deep, which can effectively prevent the wires from being damaged by pressure.

[0032] One end of the orifice plate 1 is equipped with an electrical control box 17 connected to the positive electrode wire 10 and the negative electrode wire 13, which provides synchronous pulse current to the electrodes of all the orifice 3. The electrical control box 17 is equipped with a pulse power supply. The front of the electrical control box 17 is equipped with a control knob for turning the pulse power supply on and off and an indicator light for displaying the working status. The output voltage range of the pulse power supply is 100 to 1000V, and the pulse width is 10 to 1000μs. The parameters can be adjusted by adjusting the knob.

[0033] One end of the base plate 5 is hinged to one end of the perforated plate 1. The other end of the base plate 5 is equipped with an adjustment mechanism for adjusting the tilt angle of the perforated plate 1. The adjustment mechanism includes an adjustment wheel 4 rotatably connected to the end of the base plate 5 via a rotating shaft 16, and an adjustment handle 7 rotatably connected to the circular surface of the adjustment wheel 4 away from the rotating shaft 16 at a position off-center. The surface of the adjustment handle 7 is provided with anti-slip texture to facilitate manual rotation of the adjustment wheel 4. The circular surface of the adjustment wheel 4 is provided with a guide groove 8 that is inclined from the inside to the outside. A fixing post 9 is fixed to one end of the perforated plate 1 near the adjustment wheel 4, and one end of the fixing post 9 extends into the guide groove 8 and slides with it. When the adjustment wheel 4 rotates, the guide groove 8 pushes the fixing post 9 to move up and down, thereby causing the perforated plate 1 to tilt around the hinge point, thus achieving the purpose of conveniently adjusting the tilt angle of the perforated plate 1.

[0034] In this embodiment, the tilt angle of the guide groove 8 is set to 30-45 degrees, so that the adjusting wheel 4 can drive the fixed column 9 to move more smoothly after rotation.

[0035] In this embodiment, a cover plate 2 is hinged to one side of the top surface of the orifice plate 1. The length and width of the cover plate 2 are the same as those of the orifice plate 1. The side of the cover plate 2 away from the hinge point with the orifice plate 1 is fixed to the orifice plate 1 by a latch. The cover plate 2 is made of transparent polypropylene. A silicone sealing ring is provided on the inner side of the cover plate 2 to fit the edge of the top surface of the orifice plate 1, which can seal the cavity 3, prevent solution evaporation or external contamination during the transfection process, and at the same time avoid leakage of electric arc generated during electrical stimulation.

[0036] Example 2:

[0037] Combination Figure 1 - Figure 5 This embodiment, based on embodiment 1, further improves upon the following: a positioning component for maintaining the tilt angle of the orifice plate 1 is installed between the adjusting wheel 4 and the base plate 5. The positioning component includes a rubber ball 18 fixed on the circular surface of the adjusting wheel 4 near the base plate 5 and multiple locking holes 15 corresponding to the movement trajectory of the rubber ball 18 at the end of the base plate 5. The rubber ball 18 is located at a position off-center from the circular surface of the adjusting wheel 4. When the adjusting wheel 4 rotates to the target angle, the rubber ball 18 is locked into the corresponding locking hole 15 under the action of elasticity, thereby positioning the adjusting wheel 4 and fixing the tilt angle of the orifice plate 1, preventing the orifice plate 1 from shaking during the extraction of cell solution. Through the cooperation of the adjusting wheel and the guide groove, the tilt angle of the orifice plate is adjusted, and the cell solution is made to converge towards the lower end when extracting cell solution, which can significantly reduce the residual rate.

[0038] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A transfection plate for transient cell transfection, characterized in that, It includes an orifice plate and a bottom plate disposed on the bottom surface of the orifice plate. The top surface of the orifice plate has multiple cavities, and positive and negative electrodes are disposed in the cavities. The bottom surface of the orifice plate is embedded with power supply lines for connecting the positive and negative electrodes. One end of the base plate is hinged to one end of the perforated plate, and the other end of the base plate is equipped with an adjustment mechanism for adjusting the tilt angle of the perforated plate. The adjustment mechanism includes an adjustment wheel rotatably connected to the end of the base plate via a rotating shaft, and an adjustment handle rotatably connected to the adjustment wheel at a position off-center on the circular surface away from the rotating shaft. The circular surface of the adjustment wheel is provided with a guide groove that is inclined from the inside to the outside. A fixing post is fixed to one end of the perforated plate near the adjustment wheel, and one end of the fixing post extends into the guide groove and slides therewith. A positioning component for maintaining the tilt angle of the orifice plate is also installed between the adjusting wheel and the base plate.

2. The transfection plate for transient cell transfection according to claim 1, characterized in that, A cover plate is hinged to one side of the top surface of the perforated plate. The length and width of the cover plate are the same as those of the perforated plate. The side of the cover plate away from the hinge point with the perforated plate is fixed to the perforated plate by a latch.

3. The transfection plate for transient cell transfection according to claim 1, characterized in that, The positioning component includes a rubber ball fixed on the circular surface of the adjusting wheel near the base plate and multiple locking holes opened at the end of the base plate for engaging the rubber ball. The rubber ball is located off-center from the center of the circular surface of the adjusting wheel.

4. The transfection plate for transient cell transfection according to claim 1, characterized in that, The power supply line includes a positive electrode wire and a negative electrode wire arranged along the length of the orifice plate. Multiple positive electrode branches are connected to the positive electrode wire along the width of the orifice plate. The positive electrode in the cavity is connected to the positive electrode branch. Multiple negative electrode branches are connected to the negative electrode wire along the width of the orifice plate. The negative electrode in the cavity is connected to the negative electrode branch. The bottom surface of the orifice plate has grooves for fitting the positive electrode wire, the negative electrode wire, the positive electrode branch, and the negative electrode branch.

5. A transfection plate for transient cell transfection according to claim 4, characterized in that, One end of the orifice plate is equipped with an electrical control box that is connected to the positive and negative wires. The electrical control box contains a pulse power supply, and the front of the electrical control box is equipped with a control knob for turning the pulse power supply on and off and an indicator light for displaying the working status.

6. A transfection plate for transient cell transfection according to claim 1, characterized in that, The tilt angle of the guide groove is set to 30-45 degrees.

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