An oocyte flusher and flushing device

Abstract

The utility model relates to cell flushing technical field, concretely relates to a kind of oocyte flusher and flushing device, and the flusher includes inlet pipe, water distributor, enclosed cover;The enclosed cover includes side wall and top wall;The inlet pipe is connected in the upper portion of top wall, and inlet pipe is used to communicate with flushing water source;The water distributor is fixed in the side wall;Flush water hole is distributed on the water distributor.The cell filter of the utility model avoids the invasion of foreign matter outside during flushing process;And simple operation, save flushing fluid.

Description

Technical Field

[0001] This utility model relates to the field of cell flushing technology, specifically to an oocyte flushing device and flushing apparatus. Background Technology

[0002] In the field of biotechnology, industrialized biological cloning engineering requires embryo factories with large quantities of oocytes. Oocytes from animals like cattle and horses require live-cell retrieval. This primarily involves using instruments to retrieve oocytes from the ovaries of cattle or horses, collecting follicular fluid containing the eggs. The follicular fluid is then filtered using an oocyte filter and rinsed to obtain the oocytes. Current technologies often use methods such as… Figure 1 The oocyte filter shown filters oocytes, with the oocytes remaining at the top of the filter and impurities draining from the bottom. After filtration, hold the filter handle and transfer it above the culture dish, invert the filter 180 degrees and tilt it upwards. Then, use a syringe to draw up washing solution and gently rinse the cells into the culture dish little by little. While this process is more convenient than previous methods, it still suffers from slow cell rinsing, low efficiency, and inconvenient operation. Furthermore, the oocytes are directly exposed to an open space during rinsing, potentially leading to oocyte contamination. Utility Model Content

[0003] The purpose of this invention is to provide an oocyte flushing device and flushing apparatus to solve the problems mentioned in the background art.

[0004] An oocyte flushing device includes an inlet pipe, a water distributor, and a sealing cover; the sealing cover includes a side wall and a top wall; the inlet pipe is connected to the upper part of the top wall and is used to communicate with a flushing water source; the water distributor is fixed in the side wall; the water distributor has flushing water holes distributed on it.

[0005] Furthermore, the shape of the water distributor is the same as the cross-sectional shape formed by the sidewalls; the cross-sectional shape formed by the sidewalls is circular or square.

[0006] Furthermore, the flushing water holes are evenly arranged on the flusher.

[0007] Furthermore, the diameter of the flushing water hole is between 0.1mm and 5mm. Preferably, it is 0.1mm, 0.2mm, or 0.3mm.

[0008] Furthermore, the lower part of the sidewall is provided with a step that cooperates with the oocyte filter.

[0009] Furthermore, the top wall is conical, and there is a flushing water chamber between the top wall and the water distributor.

[0010] Furthermore, the rinsing water source is a syringe, and the water inlet pipe is matched with the syringe outlet.

[0011] Furthermore, the flushing water source includes a flushing pump, a flushing tank, and a flushing liquid.

[0012] Furthermore, the flushing water source is a pressurized flushing tank.

[0013] This utility model also provides an oocyte rinsing device, including the aforementioned rinser, and further including a syringe, a culture dish, and a cell filter; the syringe contains rinsing fluid and is connected to an inlet pipe; the cell filter is connected to the oocyte rinser; the culture dish is located below the cell filter.

[0014] Furthermore, the oocyte flushing device is tilted upwards from the horizontal; the angle between the oocyte flushing device and the horizontal is 135 degrees.

[0015] The working principle is as follows: draw the rinsing solution into the syringe, place the cell filter above the culture dish, turn the cell filter upside down, then place the cell filter rinser on the cell filter, tilt the cell filter upwards at 135 degrees, connect the syringe to the oocyte rinser, and slowly push the syringe. The rinsing solution is evenly sprayed onto the filter screen of the cell filter through the water distributor. The water mist washes the cells on the other side of the filter into the culture dish below.

[0016] Alternatively, the oocyte flushing device can be connected to a mini flushing water pump for flushing, or connected to a high-pressure tank for flushing.

[0017] By adopting the above technical solution, the rinsing of the cell filter was achieved.

[0018] Compared with the prior art, the beneficial effects of this utility model are:

[0019] 1. The cell filter flushing device covers the cell filter to form a closed space, preventing foreign objects from entering during the flushing process;

[0020] 2. The water distributor can spray water directly onto all areas above the filter, making it simple to operate, highly efficient, and saving on flushing fluid. Attached Figure Description

[0021] Figure 1 This is a three-dimensional structural diagram of the cell filter of this utility model;

[0022] Figure 2 This is a three-dimensional structural diagram of the cell filter flushing device according to Embodiment 1 of this utility model;

[0023] Figure 3 for Figure 2Front view of the oocyte flushing device in the image;

[0024] Figure 4 for Figure 3 A cross-sectional view of the oocyte flushing device along the BB.

[0025] Figure 5 This is a three-dimensional structural diagram of the cell washing device according to Embodiment 2 of this utility model;

[0026] Figure 6 This is a schematic diagram of the flushing water connection structure of the oocyte flushing device in Embodiment 3 of this utility model;

[0027] Figure 7 This is a schematic diagram of the flushing water connection structure of the oocyte flushing device in Embodiment 4 of this utility model.

[0028] In the diagram: 1. Water inlet pipe; 2. Water distributor; 3. Sealing cover; 21. Rinse water hole; 31. Side wall; 32. Top wall; 33. Rinse water chamber; 100. Oocyte rinser; 200. Culture dish; 300. Cell filter; 400. Miniature rinse pump; 500. Rinse tank; 600. Rubber pressure bladder; 700. Air pressure pump. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] Example 1

[0031] Please see Figure 2-4 This invention provides an oocyte flushing device, the flushing device 100 including a water inlet pipe 1, a water distributor 2, and a sealing cover 3; the sealing cover 3 includes a side wall 31 and a top wall 32; the water inlet pipe 1 is connected to the upper part of the top wall 32 and is used to communicate with a flushing water source; the water distributor 2 is fixed in the side wall 31; the water distributor 2 has flushing water holes 21 distributed on it. The flushing solution is sprayed out from the flushing water holes 21 and can be directly sprayed onto the cell filter.

[0032] Furthermore, the shape of the water distributor 2 is the same as the cross-sectional shape enclosed by the side wall 31; the cross-sectional shape enclosed by the side wall 31 is circular or square.

[0033] Furthermore, the flushing water holes 21 are evenly arranged on the water distributor 2.

[0034] Furthermore, the diameter of the flushing water hole is between 0.05mm and 5mm. Preferably, the diameter of the flushing water hole is 0.05mm, 0.1mm, 0.2mm, 0.5mm, 0.6mm, or 0.8mm.

[0035] Furthermore, the lower part of the sidewall 31 is provided with a step 311 that cooperates with the cell filter.

[0036] Furthermore, the top wall 32 is conical, and there is a flushing water chamber 33 between the top wall 32 and the water distributor 2. During flushing, the flushing water chamber 33 is filled with flushing fluid, and under pressure, the flushing fluid is sprayed out from the flushing hole.

[0037] Furthermore, the rinsing water source is a syringe (not shown), and the water inlet pipe 1 is matched with the syringe outlet. Alternatively, the water inlet pipe 1 and the syringe can be connected via a flexible hose.

[0038] Example 2

[0039] like Figure 5 Based on Example 1, this invention further provides a cell rinsing device, including the cell filter rinser 100 described in Example 1, and also including a syringe (not shown), a culture dish 200, and a cell filter 300; the syringe contains rinsing fluid and is connected to the inlet pipe of the cell filter rinser 100; the cell filter 300 is connected to the cell filter rinser 100; the culture dish 200 is located below the cell filter 300. Furthermore, the cell filter rinser 100 is tilted upwards from the horizontal; the angle between the cell filter rinser and the horizontal is 135 degrees.

[0040] Furthermore, the culture dish 200 can be replaced with other containers, such as 50 ml or 100 ml centrifuge tubes; other containers that can collect cells can also be used.

[0041] like Figure 1 The cell filter 300 has a filter screen to filter cells and remove impurities, and it also has a handle for easy operation. The size of the mesh size of the cell filter 300 can be determined by those skilled in the art based on the size of the cells to be filtered. The cell filter 300 can be made of plastic or metal.

[0042] The cell rinsing process is as follows: Draw rinsing solution into the syringe, place the cell filter 300 above the culture dish 200, invert the cell filter 300, and then place the cell filter rinser 100 on top of the cell filter 300. Tilt the cell filter 300 upwards at a 135-degree angle, connect the syringe to the cell filter rinser 100, and slowly push the syringe. The rinsing solution is evenly sprayed onto the filter screen of the cell filter 300 through the water distributor. The sprayed water rinses the cells on the other side of the filter into the culture dish 300 below. When the liquid in the syringe is used up, more rinsing solution can be drawn up and the process repeated several times. The specific number of rinsing cycles can be determined based on the actual situation.

[0043] Example 3

[0044] Further improvements were made based on Example 2, with the flushing water source replaced by a micro flushing pump 400. The inlet of the micro flushing pump 400 was connected to the flushing tank 500 via a pipe, and the outlet of the micro flushing pump 400 was connected to the inlet of the oocyte flushing device 100.

[0045] The flushing tank 500 contains flushing fluid; flushing is performed by turning on the miniature flushing pump 400. Furthermore, a regulating valve can be installed at the outlet of the miniature flushing pump 400 for flow regulation. A flushing fluid filling port is located at the top of the flushing tank 500. Additionally, a safety pressure relief valve can be installed at the outlet of the miniature flushing pump 400. The inlet of the miniature flushing pump 400 is connected to the bottom of the flushing tank 500 via a pipe. The miniature flushing pump 400 is also equipped with a start / stop switch; the switch is turned on during flushing and turned off when not in use.

[0046] Example 4

[0047] Further improvements are made based on embodiments 2 and 3. Specifically, the lower part of the rinsing tank 500 is directly connected to the inlet of the oocyte rinsing device 100 via a pipe. Preferably, a flow regulating valve can also be installed on the pipe between the rinsing tank 500 and the oocyte rinsing device 100. The rinsing tank 500 is equipped with a rubber pressure bladder 600, and a pneumatic pump 700 is installed on the rubber pressure bladder 600. The rubber pressure bladder 600 is connected to the top of the rinsing tank 500 via a pipe. A liquid inlet is also provided on the top of the rinsing tank 500, which is sealed with a lid during operation. A pressure sensor is installed in the upper space inside the rinsing tank 500. When the pressure in the rinsing tank 500 is lower than a first pressure, the controller controls the pneumatic pump 700 to start; when the pressure in the rinsing tank 500 is higher than a second pressure, the pneumatic pump 700 stops working. The rubber pressure bladder 600 is elastic, increasing in volume when the pressure is high and decreasing in volume when the pressure is low, thus playing a pressure stabilizing role. These are all existing technologies and will not be described in detail further.

[0048] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. An oocyte flushing device, characterized in that, The flushing device includes an inlet pipe, a water distributor, and a closed cover; the closed cover includes a side wall and a top wall; the inlet pipe is connected to the upper part of the top wall and is used to communicate with the flushing water source; the water distributor is fixed in the side wall; the water distributor has flushing water holes distributed on it.

2. The oocyte flushing device according to claim 1, characterized in that, The shape of the water distributor is the same as the cross-sectional shape formed by the sidewalls; the cross-sectional shape formed by the sidewalls is circular or square.

3. The oocyte flushing device according to claim 1, characterized in that, The flushing holes are evenly arranged on the flusher.

4. The oocyte flushing device according to claim 1, characterized in that, The diameter of the flushing water hole is between 0.05mm and 5mm.

5. The oocyte flushing device according to claim 1, characterized in that, The top wall is conical, and there is a flushing water chamber between the top wall and the water distributor.

6. The oocyte flushing device according to claim 1, characterized in that, The rinsing water source is a syringe, and the water inlet pipe is matched with the syringe outlet.

7. A cell flushing device, characterized in that, The device includes the oocyte flushing device as described in claim 6; the syringe contains flushing fluid and is connected to an inlet pipe; a cell filter is connected to the cell filter flushing device; and a culture dish is located below the cell filter.

8. The cell washing device according to claim 7, characterized in that, The oocyte flushing device is tilted upwards at a 135-degree angle to the horizontal.

9. The cell washing device according to claim 8, characterized in that, The petri dish can be replaced with a 50 ml or 100 ml centrifuge tube.

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