A device for integrating inoculation and sampling of a sterile culture of actinomyces in a flask

Abstract

The utility model discloses a kind of flask inoculation sampling integration devices of actinomycete aseptic culture, including flask main part, bottle mouth sealing assembly, inoculation component and sampling component;Flask main part top is equipped with bottle mouth, bottle mouth sealing assembly seals and covers in bottle mouth place;Inoculation component includes inoculation tube and first sterile filter piece, inoculation tube penetrates sealing assembly and one end extends to flask main part inside, the other end is exposed, and first sterile filter piece is located at inoculation tube exposed end;Sampling component includes sampling tube and second sterile filter piece, sampling tube penetrates sealing assembly and one end extends to flask main part bottom, the other end is exposed, and sampling valve is equipped on sampling tube, and second sterile filter piece is located at sampling tube exposed end.The utility model realizes the integration aseptic operation of inoculation and sampling, avoids the bacterial pollution caused by repeatedly opening sealing structure, simplifies operation process, improves the stability and efficiency of actinomycete culture, applicable to actinomycete laboratory culture and small-scale fermentation scene.

Description

Technical Field

[0001] This utility model relates to the field of microbial culture equipment technology, and in particular to an integrated device for shake flask inoculation and sampling for aseptic culture of actinomycetes. Background Technology

[0002] Actinomycetes are an important microbial resource with wide applications in antibiotics, enzyme preparations, and bioactive substances. A sterile environment is crucial for successful actinomycete cultivation, while inoculation and sampling are two core operational steps in the process.

[0003] In existing technologies, actinomycete shake-flask culture typically uses ordinary Erlenmeyer flasks, with the mouth sealed with gauze or a sealing plug. Inoculation requires opening the seal, injecting the inoculum into the shake flask, and then resealing it; sampling also requires opening the seal and using a pipette or sampling spoon to extract the culture. This method has significant drawbacks: repeated opening of the seal easily allows external bacteria to enter the shake flask, causing culture contamination; the procedure is cumbersome and inefficient; and sampling easily disturbs the culture system, affecting the growth of the actinomycetes.

[0004] Therefore, it is necessary to design a sterile device that can integrate inoculation and sampling to solve the problems of high risk of contamination and complex operation in existing procedures. Utility Model Content

[0005] To address the technical problems of easy contamination and cumbersome procedures in the inoculation and sampling operations of existing actinomycete shake flask cultures, this utility model provides an integrated device for shake flask inoculation and sampling in aseptic culture of actinomycetes, which realizes integrated operation in an aseptic environment, reduces the risk of contamination, and simplifies the operation process.

[0006] To achieve the above objectives, this utility model provides an integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture, comprising: The shake flask body has a bottle mouth at the top and a culture chamber inside; A bottle mouth sealing assembly, wherein the bottle mouth sealing assembly is detachably sealed and fitted onto the bottle mouth; An inoculation assembly includes an inoculation tube and a first sterile filter element. The inoculation tube passes through the bottle mouth sealing assembly, with one end extending into the culture chamber and the other end exposed outside the bottle mouth sealing assembly. The first sterile filter element is fixed to the exposed end of the inoculation tube. The sampling assembly includes a sampling tube and a second sterile filter. The sampling tube passes through the bottle mouth sealing assembly, with one end extending to the bottom of the culture chamber and the other end exposed outside the bottle mouth sealing assembly. The exposed section of the sampling tube is provided with a sampling valve, and the second sterile filter is fixed to the exposed end of the sampling tube.

[0007] Preferably, the bottle neck sealing assembly includes a sealing plug.

[0008] Preferably, the end of the inoculation tube extending into the culture chamber has a slanted cut, and the slanted cut faces the inner wall of the shake flask body.

[0009] Preferably, the end of the sampling tube extending to the bottom of the culture chamber is provided with a filter screen, and the pore size of the filter screen is 0.2-0.5μm.

[0010] Preferably, both the first and second sterile filter elements are polytetrafluoroethylene (PTFE) membrane filters with a pore size of 0.22 μm.

[0011] Preferably, both the inoculation tube and the sampling tube are sealed to the bottle mouth sealing assembly through a sealing ring.

[0012] Preferably, the sampling valve is a sterile diaphragm valve, and the exposed end of the sampling tube is also provided with a quick connector adapted to the sampling instrument.

[0013] Preferably, the outer wall of the shaker body is provided with capacity scale lines.

[0014] This utility model discloses an integrated shake-flask inoculation and sampling device for aseptic culture of actinomycetes, which has the following beneficial effects: (1) It realizes the integrated aseptic operation of inoculation and sampling, eliminating the need to repeatedly open the bottle mouth sealing structure, fundamentally reducing the risk of contamination by miscellaneous bacteria and ensuring the purity of actinomycete culture.

[0015] (2) The structure of the inoculation tube and the sampling tube is reasonable. The inoculum is injected smoothly during inoculation and the bottom culture can be obtained during sampling. The tube is also blocked, making the operation convenient and efficient.

[0016] (3) Each component is detachable and washable, the sterile filter is replaceable, the device has a high reusability rate, and it is suitable for multiple uses in laboratory and small-scale fermentation scenarios.

[0017] The technical solution of this utility model will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0018] Figure 1 This is an overall structural diagram of the integrated inoculation and sampling device according to an embodiment of the present invention.

[0019] In the picture: 1. Shaking flask body; 2. Bottle mouth sealing assembly; 3. Inoculation assembly; 31. Inoculation tube; 32. First sterile filter element; 4. Sampling assembly; 41. Sampling tube; 42. Sampling valve; 43. Second sterile filter element; 44. Quick connector; 5. Volume scale. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the present utility model embodiments clearer, the present utility model embodiments will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model embodiments and are not intended to limit the present utility model embodiments. All other embodiments obtained by those skilled in the art based on the embodiments in this application without creative effort are within the scope of protection of this application. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout.

[0021] It should be noted that the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or server that includes a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to such process, method, product, or device.

[0022] Similar labels and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0023] Example 1 An integrated device for aseptic culture of actinomycetes using shake-flask inoculation and sampling, the structure of which is as follows: Figure 1 As shown, it includes a shake flask body 1, a bottle mouth sealing component 2 for sealing the shake flask, an inoculation component 3 for inoculating actinomycetes, and a sampling component 4 for sampling actinomycete bacterial solution.

[0024] The shake flask body 1 is made of borosilicate glass. The outer wall of the shake flask body 1 has volume graduation lines 5. The top of the shake flask body 1 has a bottle mouth, forming an internal culture chamber for containing culture medium and actinomycetes. A removable bottle mouth sealing component 2 is provided at the bottle mouth, which can be a food-grade silicone sealant.

[0025] The inoculation assembly 3 includes an inoculation tube 31, one end of which extends into the culture chamber and has an oblique cut facing the inner wall to prevent splashing of the inoculum during inoculation. The sampling assembly 4 includes a sampling tube 41, one end of which extends to the bottom of the culture chamber and has a 0.2-0.5μm filter screen to prevent actinomycete hyphae from clogging the tube. The filter screen is a commonly used existing structure and is not shown in the figure. The bottle mouth sealing assembly 2 has a through hole that allows the inoculation tube 31 and the sampling tube 41 to pass through; the through hole is not shown. Sealing rings (not shown in the figure, a common existing technology) are provided at the penetration points of the inoculation tube 31 and the bottle mouth sealing assembly 2, as well as the sampling tube 41 and the bottle mouth sealing assembly 2, to form a sealing structure.

[0026] The exposed end of the inoculation tube 31 is equipped with a first sterile filter element 32. The first sterile filter element 32 is a cylindrical membrane filter with a 0.22μm polytetrafluoroethylene filter membrane, which allows the actinomycete culture to pass through under micro-pressure while preventing other contaminants from passing through. The bottom of the filter is threadedly connected to the exposed end of the inoculation tube 31, facilitating the disassembly, cleaning, or replacement of the first sterile filter element 32.

[0027] A sampling valve 42 is provided in the middle of the exposed section of the sampling tube 41. The sampling valve 42 is a sterile diaphragm valve. A quick connector 44 is provided at the top of the exposed end of the sampling tube 41. The quick connector 44 is a Luer connector structure. The quick connector 44 is connected to the exposed end of the sampling tube 41 by thread and is compatible with pipettes, sampling bottles and other instruments. The top of the quick connector 44 is provided with a second sterile filter element 43. The second sterile filter element 43 has the same structure as the first sterile filter element 32 and is a cylindrical filter membrane filter. The bottom of the filter is connected to the top of the quick connector 44 by threads.

[0028] In the process of using this invention, the prepared culture medium is first injected into the shake flask body 1, and the amount is controlled by the volume scale line 5. The bottle mouth sealing component 2 is then closed. The inoculum solution is injected into the culture chamber through the inoculation tube 31. The first sterile filter element 32 filters out external bacteria, and the oblique cut prevents the inoculum solution from splashing. The device is placed on a shaker for vibration culture. When sampling is required during the culture process, the sampling instrument is connected to the quick connector 44, the sampling valve 42 is opened, and the bottom culture is extracted through the sampling tube 41. The filter screen prevents mycelial blockage. After sampling, the sampling valve 42 is closed, and the second sterile filter element 43 is connected to the quick connector 44. After the culture is completed, all components are disassembled, cleaned, and the sterile filter element is replaced. The device can then be reused.

[0029] Therefore, this utility model provides an integrated shake flask inoculation and sampling device for aseptic culture of actinomycetes, which can realize integrated aseptic operation of inoculation and sampling, avoid contamination by miscellaneous bacteria caused by repeated opening of the sealed structure, simplify the operation process, improve the stability and efficiency of actinomycete culture, and is suitable for laboratory culture and small-scale fermentation of actinomycetes.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it. Although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solution of this utility model, and these modifications or equivalent substitutions cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of this utility model.

Claims

1. An integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture, characterized in that, include: The shake flask body has a bottle mouth at the top and a culture chamber inside; A bottle mouth sealing assembly, wherein the bottle mouth sealing assembly is detachably sealed and fitted onto the bottle mouth; An inoculation assembly includes an inoculation tube and a first sterile filter element. The inoculation tube passes through the bottle mouth sealing assembly, with one end extending into the culture chamber and the other end exposed outside the bottle mouth sealing assembly. The first sterile filter element is fixed to the exposed end of the inoculation tube. The sampling assembly includes a sampling tube and a second sterile filter. The sampling tube passes through the bottle mouth sealing assembly, with one end extending to the bottom of the culture chamber and the other end exposed outside the bottle mouth sealing assembly. The exposed section of the sampling tube is provided with a sampling valve, and the second sterile filter is fixed to the exposed end of the sampling tube.

2. The integrated device for aseptic culture of actinomycetes using shake flasks for inoculation and sampling according to claim 1, characterized in that: The bottle neck sealing assembly includes a sealing plug.

3. The integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture according to claim 2, characterized in that, The inoculation tube has a slanted cut at one end extending into the culture chamber, and the slanted cut faces the inner wall of the shake flask body.

4. The integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture according to claim 3, characterized in that: The sampling tube is provided with a filter screen at one end extending to the bottom of the culture chamber, and the filter screen has a pore size of 0.2-0.5μm.

5. The integrated device for aseptic culture of actinomycetes using shake flasks for inoculation and sampling according to claim 4, characterized in that: Both the first and second sterile filter elements are polytetrafluoroethylene (PTFE) membrane filters with a pore size of 0.22 μm.

6. The integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture according to claim 5, characterized in that: Both the inoculation tube and the sampling tube are sealed to the bottle mouth sealing assembly through a sealing ring.

7. The integrated device for shake-flask inoculation and sampling of aseptic actinomycete culture according to claim 6, characterized in that: The sampling valve is a sterile diaphragm valve, and the exposed end of the sampling tube is also equipped with a quick connector adapted to the sampling instrument.

8. The integrated device for aseptic culture of actinomycetes using shake flasks for inoculation and sampling according to claim 7, characterized in that: The outer wall of the shaker body is provided with capacity scale lines.

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