A multi-channel ultrasonic nucleic acid sample lysis processing device

Abstract

The utility model discloses a kind of multi-channel ultrasonic nucleic acid sample cracking processing device, it is related to ultrasonic technology field, including pneumatic four-claw chuck and disc tool head, the middle part of the pneumatic four-claw chuck is provided with ultrasonic vibrator, and the output end of ultrasonic vibrator is connected with disc tool head, the outside of disc tool head is equipped with recess, the output end of the pneumatic four-claw chuck is placed with pusher assembly, and the top of pusher assembly is provided with ring. The multi-channel ultrasonic nucleic acid sample cracking processing device is set by disc tool head, since the test tube is evenly arranged in the recess of the outer circumferential surface of disc tool head, after the ultrasonic vibrator located in the center emits ultrasonic energy, the ultrasonic wave is converted from the longitudinal direction of ultrasonic vibrator to the radial direction on disc tool head by disc tool head, and evenly diverges transmission as circular arc, when reaching the recess, each test tube receives uniform ultrasonic energy, so as to improve test accuracy.

Description

Technical Field

[0001] This utility model relates to the field of ultrasonic technology, specifically a multi-channel ultrasonic nucleic acid sample lysis processing device. Background Technology

[0002] Nucleic acid sample lysis mainly involves three core methods: physical lysis, chemical lysis, and enzymatic lysis. These methods can be used individually or in combination to efficiently break up samples and release nucleic acids. For example, ultrasonic lysis is a type of physical lysis. Its core principle is the cavitation effect: when ultrasound propagates in a liquid, it generates alternating high-pressure and low-pressure zones. Microbubbles form in the low-pressure zones and then rapidly collapse in the high-pressure zones, releasing enormous energy to break up cells. In addition, excessive ultrasound energy can cause nucleic acid breakage due to local high temperatures and mechanical forces. Therefore, in actual operation, it is necessary to strictly control the power, time, and interval of ultrasound.

[0003] Currently, ultrasonic nucleic acid sample lysis processing devices in the industry are generally of two types: planar and disc. In the planar type, the ultrasonic transducer emits ultrasonic waves, which are transmitted to the test tubes in a straight line or arc through the tool head. As a result, the test tubes closer to the ultrasonic transducer receive a large amount of ultrasonic waves, while the adjacent test tubes, due to the greater distance, receive less energy, leading to uneven ultrasonic energy and uneven nucleic acid lysis, which cannot meet the lysis requirements. In the disc type, the sound wave energy emitted from the ultrasonic transducer reaches the bottom of the test tube of the disc tool head. This means that it can only process small-volume samples and cannot process multiple samples, which has certain limitations. Utility Model Content

[0004] The purpose of this invention is to provide a multi-channel ultrasonic nucleic acid sample lysis processing device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a multi-channel ultrasonic nucleic acid sample lysis processing device, comprising a pneumatic four-jaw clamp and a disc tool head, wherein an ultrasonic transducer is disposed in the middle of the pneumatic four-jaw clamp, and the output end of the ultrasonic transducer is connected to the disc tool head, a groove is provided on the outer side of the disc tool head, a pushing component is disposed at the output end of the pneumatic four-jaw clamp, and a support ring is disposed above the pushing component.

[0006] Furthermore, the axis of the pneumatic four-jaw clamp coincides with the axis of the disc tool head, and the axis of the ultrasonic transducer also coincides with the axis of the disc tool head.

[0007] Furthermore, a test tube extends through the interior of the ring, and a flange is provided at the upper end of the test tube.

[0008] Furthermore, the curvature of the groove matches the curvature of the outer circumference of the test tube, and the groove is equidistantly distributed circumferentially along the outer side of the disc tool head.

[0009] Furthermore, the diameter of the flange is larger than the inner diameter of the support ring, and the support ring and the pushing assembly are integrated into one structure.

[0010] Furthermore, the pushing component includes a clamping plate and a positioning groove. The output end of the pneumatic four-jaw clamp is equipped with a clamping plate, and the inner side of the clamping plate is provided with a positioning groove.

[0011] Furthermore, the positioning groove is arc-shaped, and the curvature of the positioning groove is consistent with the curvature of the groove.

[0012] Furthermore, the positioning groove abuts against the test tube, and the test tube corresponds one-to-one with the groove.

[0013] This invention provides a multi-channel ultrasonic nucleic acid sample lysis processing device, which has the following beneficial effects: 1. This utility model, through the setting of the disc tool head, since the test tubes are evenly placed in the groove on the outer circumference of the disc tool head, after the ultrasonic transducer at the center emits ultrasonic energy, the ultrasonic waves are converted from the longitudinal direction of the ultrasonic transducer to the radial direction on the disc tool head and evenly dispersed in an arc. When they reach the groove, the ultrasonic energy received by each test tube is uniform, thereby improving the test accuracy. 2. This utility model can be designed with a large number of grooves according to different needs, so as to process a large number of samples at one time. It can also be designed with disc tool heads of different thicknesses according to the sample capacity to meet the needs of large-capacity sample processing. 3. With the addition of a support ring, the bottom of the flange can be supported simply by inserting the test tube into the support ring, thus quickly positioning the test tube initially. Then, when the pneumatic four-jaw clamp is activated, all the clamping plates can be driven to press the test tube firmly onto the disc tool head, thereby improving the convenience of test tube clamping. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall three-dimensional structure of a multi-channel ultrasonic nucleic acid sample lysis processing device according to the present invention; Figure 2 This is a three-dimensional structural diagram of the driving component of a multi-channel ultrasonic nucleic acid sample lysis processing device according to the present invention; Figure 3 This is a schematic diagram of the test tube structure of a multi-channel ultrasonic nucleic acid sample lysis processing device according to the present invention.

[0015] In the figure: 1. Pneumatic four-jaw clamp; 2. Ultrasonic transducer; 3. Disc tool head; 4. Groove; 5. Support ring; 6. Test tube; 7. Flange; 8. Pushing assembly; 801. Clamping plate; 802. Positioning groove. Detailed Implementation

[0016] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0017] like Figures 1 to 3 As shown, a multi-channel ultrasonic nucleic acid sample lysis processing device includes a pneumatic four-jaw clamp 1 and a disc tool head 3. An ultrasonic transducer 2 is disposed in the middle of the pneumatic four-jaw clamp 1, and the output end of the ultrasonic transducer 2 is connected to the disc tool head 3. The axis of the pneumatic four-jaw clamp 1 coincides with the axis of the disc tool head 3, and the axis of the ultrasonic transducer 2 also coincides with the axis of the disc tool head 3. After the ultrasonic transducer 2 emits ultrasonic energy, the ultrasonic waves are converted from the longitudinal direction of the ultrasonic transducer 2 to the radial direction on the disc tool head 3, and evenly dispersed in all directions. A groove 4 is formed on the outer side of the disc tool head 3. A pushing component 8 is disposed at the output end of the pneumatic four-jaw clamp 1, and a support ring 5 is disposed above the pushing component 8. A test tube 6 passes through the inside of the support ring 5, and a flange 7 is provided at the upper end of the test tube 6. The curvature of the groove 4 matches the curvature of the outer circumference of the test tube 6, and the groove 4 extends along the outer side of the disc tool head 3. The test tubes 6 are evenly distributed in a circular pattern. When the ultrasonic waves reach the groove 4, the ultrasonic energy received by each test tube 6 is uniform, thereby improving the test accuracy. The diameter of the flange 7 is larger than the inner diameter of the support ring 5, and the support ring 5 and the pushing component 8 are integrated. After the test tube 6 is inserted into the support ring 5, the support ring 5 can support the bottom of the flange 7, thereby quickly positioning the test tube 6. The pushing component 8 includes a clamping plate 801 and a positioning groove 802. The output end of the pneumatic four-jaw clamp 1 is equipped with a clamping plate 801, and the inner side of the clamping plate 801 is provided with a positioning groove 802. The positioning groove 802 is arc-shaped, and the arc of the positioning groove 802 is consistent with the arc of the groove 4. The positioning groove 802 can limit the position of the test tube 6. The positioning groove 802 abuts against the test tube 6, and the test tube 6 corresponds one-to-one with the groove 4. When the pneumatic four-jaw clamp 1 is started, all the clamping plates 801 can be driven to press the test tube 6 onto the disc tool head 3, thereby improving the convenience of clamping the test tube 6.

[0018] In summary, when using this multi-channel ultrasonic nucleic acid sample lysis processing device, firstly, according to the size and quantity of the sample and the experimental requirements, select a disc tool head 3 with a specific thickness and number of grooves 4. Next, insert the test tube 6 into the support ring 5 to support the bottom of the flange 7, thereby quickly and initially positioning the test tube 6. Secondly, start the pneumatic four-jaw clamp 1, which drives all the clamping plates 801 to press the test tube 6 onto the disc tool head 3, thereby improving the convenience of clamping the test tube 6. Then, after the ultrasonic generator controls the ultrasonic transducer 2 to emit ultrasonic energy, the ultrasonic waves are converted from the longitudinal direction of the ultrasonic transducer 2 to the radial direction on the disc tool head 3, and are evenly dispersed in an arc. When they reach the grooves 4, the ultrasonic energy received by each test tube 6 is uniform, thereby improving the experimental accuracy. Finally, after the lysis operation, control the pneumatic four-jaw clamp 1 to release the clamping plates 801 to remove the test tube 6 for replacement.

[0019] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A multi-channel ultrasonic nucleic acid sample lysis processing device, comprising a pneumatic four-jaw clamp (1) and a disc tool head (3), characterized in that, An ultrasonic transducer (2) is provided in the middle of the pneumatic four-jaw clamp (1), and a disc tool head (3) is connected to the output end of the ultrasonic transducer (2). A groove (4) is provided on the outer side of the disc tool head (3). A push assembly (8) is installed at the output end of the pneumatic four-jaw clamp (1), and a support ring (5) is provided above the push assembly (8).

2. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 1, characterized in that, The axis of the pneumatic four-jaw clamp (1) coincides with the axis of the disc tool head (3), and the axis of the ultrasonic transducer (2) also coincides with the axis of the disc tool head (3).

3. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 1, characterized in that, The test tube (6) runs through the inside of the ring (5), and the upper end of the test tube (6) is provided with a flange (7).

4. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 3, characterized in that, The curvature of the groove (4) matches the curvature of the outer circumference of the test tube (6), and the groove (4) is equidistantly distributed along the outer side of the disc tool head (3).

5. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 3, characterized in that, The diameter of the flange (7) is greater than the inner diameter of the ring (5), and the ring (5) and the push assembly (8) are integrated.

6. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 3, characterized in that, The pushing component (8) includes a clamping plate (801) and a positioning groove (802). The output end of the pneumatic four-jaw clamp (1) is provided with a clamping plate (801), and a positioning groove (802) is provided on the inner side of the clamping plate (801).

7. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 6, characterized in that, The positioning groove (802) is arc-shaped, and the curvature of the positioning groove (802) is consistent with the curvature of the groove (4).

8. The multi-channel ultrasonic nucleic acid sample lysis processing device according to claim 6, characterized in that, The positioning groove (802) abuts against the test tube (6), and the test tube (6) corresponds one-to-one with the groove (4).

Images