Mthfr gene mutation detection kit

Abstract

The utility model relates to detection kit technical field, concretely relates to MTHFR gene mutation detection kit, including detection box body, reaction subassembly and sampling subassembly. The utility model, through setting up reaction subassembly, after injecting the sample to be detected into multiple containing grooves, the output shaft of first brake motor can pass through the meshing of driving gear and driven gear, make driven gear rotate and pass through the meshing with the toothed plate, let upper layer reaction seat descend in seat hole, finally make the addition seat parallel to containing groove, ensured that the sample to be detected in multiple containing grooves will not be exposed to the outside world and lead to pollution, after that, multiple quantitative drop hoppers can send quantitative sample lysate into containing groove through liquid guide pipe and make it crack, after cracking and being filtered by filter membrane, the nucleic acid solution of impurity enters nucleic acid purification column and is centrally carried by staff to detection instrument, reduce the possibility of sample pollution in the cracking and carrying process, ensure the accuracy of detection data.

Description

Technical Field

[0001] This utility model relates to the field of detection kit technology, specifically to an MTHFR gene mutation detection kit. Background Technology

[0002] MTHFR (methylenetetrahydrofolate reductase) is a key enzyme in the folate metabolism pathway. Its gene contains common mutation sites such as C677T and A1298C. Mutations can lead to decreased enzyme activity, affecting folate metabolism and are associated with the risk of neural tube defects, cardiovascular and cerebrovascular diseases in fetuses.

[0003] Currently available reagent kits are mostly dispersive reagent combinations, requiring multiple manual sample transfers and reactions, which can easily lead to cross-contamination and inaccurate test results.

[0004] Therefore, the MTHFR gene mutation detection kit was proposed to address the problems mentioned above. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides an MTHFR gene mutation detection kit, which can solve the problem of inaccurate test results caused by contamination during multiple sample transfers due to the kit's dispersed reagent combination.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an MTHFR gene mutation detection kit, comprising a detection box, a reaction assembly, and a sampling assembly. The detection box has a sampling slot on its side wall, and the sampling assembly is disposed within the detection box and the sampling slot. The detection box has a seat hole at its upper end, and the reaction assembly is disposed within the detection box and the seat hole. The reaction assembly includes multiple nucleic acid purification columns, an upper reaction seat, and an addition seat. A toothed plate and three connecting plates are fixedly connected to the side wall of the upper reaction seat. The lower end of the addition seat overlaps the toothed plate and the three connecting plates. A first brake motor is fixedly installed on the side wall of the detection box. The output shaft of the first brake motor is fixedly sleeved with a drive gear. The drive gear meshes with a driven gear. A plate seat and a toothed plate frame are fixedly installed on the inner wall of the detection box. A through hole is opened on the back of the toothed plate frame. The driven gear meshes with the toothed plate at the through hole. A metering dripping funnel is fixedly inserted into the adding seat. A liquid guide tube is provided on the lower end face of the adding seat. Multiple receiving slots are equally spaced on the upper end face of the upper reaction seat. Multiple filter membranes corresponding to the receiving slots are embedded in the lower end face of the upper reaction seat.

[0007] Preferably, the sampling assembly includes a lower reaction seat with multiple placement holes, and multiple nucleic acid purification columns are respectively located in the multiple placement holes. The inner wall of the detection box and the sampling slot is provided with a limiting groove, and a limiting slide rail is fixedly connected in the limiting groove. The lower end of the upper reaction seat is slidably sleeved on the limiting slide rail.

[0008] Preferably, a second brake motor is fixedly installed at the center of the upper reaction seat, the output shaft of the second brake motor is fixedly connected to the lower end face of the addition seat, a transmission hole is opened on the side wall of the detection box, a driven shaft is inserted into the center of the driven gear, and the two ends of the driven shaft are respectively rotatably connected to the inner wall of the transmission hole.

[0009] Preferably, the inner wall of the seat hole is provided with guide plate grooves corresponding to the toothed plate and the three connecting plates. The side walls of the toothed plate and the three connecting plates are slidably connected to the inner walls of the four guide plate grooves, and the plate seat and the toothed plate frame are respectively corresponding to the connecting plates and the toothed plate.

[0010] Preferably, the lower outlet of the liquid guide tube matches the aperture of the plurality of receiving tanks, and the plurality of receiving tanks and the filter membrane are all vertically corresponding to the plurality of nucleic acid purification columns below.

[0011] Preferably, the upper end of the liquid guide tube is fixedly connected to the outlet of the quantitative dripping funnel, and the inner wall of the liquid guide tube is provided with an anti-liquid coating, which is a polytetrafluoroethylene coating.

[0012] Preferably, a digital display thermometer is embedded in the side wall of the detection box, and a temperature sensor is embedded in the inner wall of the detection box, the temperature sensor being electrically connected to the digital display thermometer.

[0013] Compared with the prior art, this invention provides an MTHFR gene mutation detection kit, which has the following beneficial effects: 1. By setting up the reaction assembly, after the sample to be tested is injected into multiple containment tanks, the output shaft of the first brake motor can rotate the driven gear through the meshing of the driving gear and the driven gear. Through the meshing with the toothed plate, the upper reaction seat descends in the seat hole, and finally makes the addition seat parallel to the containment tank. This ensures that the sample to be tested in multiple containment tanks will not be exposed to the outside world and cause contamination. Then, multiple quantitative dropping funnels can be used to send quantitative sample lysis solution into the containment tank through the liquid guide tube for lysis. After lysis and impurity filtration by the filter membrane, the nucleic acid solution enters the nucleic acid purification column and is centrally transported by staff to the detection instrument. This reduces the possibility of sample contamination during lysis and transportation and ensures the accuracy of the detection data.

[0014] 2. By setting an anti-liquid layer, the sample lysis solution can be prevented from remaining on the inner wall of the liquid guide tube, ensuring the accuracy of the amount of sample lysis solution added to the container each time and reducing the detection error caused by sample lysis solution adhering to the inner wall of the liquid guide tube. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a schematic diagram of the disassembled structure of this utility model; Figure 3 This is a three-dimensional structural diagram of the lower reaction seat, upper reaction seat, and addition seat of this utility model; Figure 4 This is a schematic diagram of the internal structure of the detection box in this utility model.

[0016] In the diagram: 1. Detection box; 2. Sampling slot; 3. Lower reaction seat; 4. Limiting slide rail; 5. Nucleic acid purification column; 6. First brake motor; 7. Drive gear; 8. Plate seat; 9. Upper reaction seat; 10. Receptacle; 11. Connecting plate; 12. Toothed plate; 13. Addition seat; 14. Second brake motor; 15. Quantitative dropping funnel; 16. Liquid guide tube; 17. Seat hole; 18. Guide plate groove; 19. Toothed plate frame. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: Please see Figure 1 - Figure 4 The MTHFR gene mutation detection kit in this embodiment includes a detection box 1, a reaction component and a sampling component. The side wall of the detection box 1 is provided with a sampling slot 2. The sampling component is disposed in the detection box 1 and the sampling slot 2. The upper end of the detection box 1 is provided with a seat hole 17. The reaction component is disposed in the detection box 1 and the seat hole 17. When the test box 1 is idle, the upper reaction seat 9 is parallel to the seat hole 17. At this time, the staff adds the sample to be tested into the multiple receiving slots 10. Then, the first brake motor 6 is started, and its output shaft drives the drive gear 7 to rotate. The drive gear 7 meshes with the driven gear, and the driven gear meshes with the toothed plate 12, so that the toothed plate 12 drives the upper reaction seat 9 to descend in the seat hole 17. When the lower end of one of the connecting plates 11 abuts against the bottom of the inner wall of the plate seat 8, the adding seat 13 is horizontally aligned with the seat hole 17. Then, the quantitative dropping funnel 15 can be used to transfer the sample lysis solution through the liquid guide. The sample to be tested and the sample lysis buffer are quantitatively injected into the lower container 10. The sample to be tested and the sample lysis buffer are lysed in the container 10 for 5 minutes and impurities are filtered by the filter membrane. The lysed nucleic acid solution can then enter the nucleic acid purification column 5 below. When multiple nucleic acid purification columns 5 contain lysed nucleic acid solutions, the lower reaction seat 3 can be taken out from the detection box 1 through the sampling slot 2 and sent to the detection equipment for centralized detection. This reduces the manual operation of sample lysis and filtration steps and the use of reagent bottles, while also reducing the number of times samples need to be handled for detection and reducing the possibility of sample contamination during handling.

[0019] Please see Figure 3 - Figure 4 Specifically Figure 4 The sampling assembly includes a lower reaction seat 3, which has multiple placement holes. Multiple nucleic acid purification columns 5 are located in the multiple placement holes. The inner walls of the detection box 1 and the sampling slot 2 are provided with a limiting groove. A limiting slide rail 4 is fixedly connected in the limiting groove. The lower end of the upper reaction seat 9 is slidably sleeved on the limiting slide rail 4. By setting up a sampling component, multiple nucleic acid purification columns 5 can be placed in the lower reaction seat 3. After the multiple nucleic acid purification columns 5 are filled with cracked nucleic acid solution, it is convenient for staff to move them to the detection instrument. This effectively reduces the number of times the multiple nucleic acid purification columns 5 are moved to the detection instrument, thereby avoiding cross-contamination of the nucleic acid solution in the nucleic acid purification columns 5. At the same time, the limiting slide rail 4 allows the lower reaction seat 3 to slide into the detection box 1.

[0020] Please see Figure 1 - Figure 4 Specifically Figure 3 A second brake motor 14 is fixedly installed at the center of the upper reaction seat 9. The output shaft of the second brake motor 14 is fixedly connected to the lower end face of the addition seat 13. A transmission hole is opened on the side wall of the detection box 1. A driven shaft is inserted into the center of the driven gear. The two ends of the driven shaft are rotatably connected to the inner wall of the transmission hole. By setting a second brake motor 14, the output shaft of the second brake motor 14 can drive the addition seat 13 to slide and rotate on the upper surface of the toothed plate 12 and the three connecting plates 11, so that the multiple receiving tanks 10 can rotate directly below the liquid guide tube 16 and wait for the sample lysis solution to be added.

[0021] Please see Figure 1 - Figure 4 Specifically Figure 2 The inner wall of the seat hole 17 is provided with guide plate grooves 18 corresponding to the toothed plate 12 and the three connecting plates 11. The side walls of the toothed plate 12 and the three connecting plates 11 are slidably connected to the inner walls of the four guide plate grooves 18 respectively. The plate seat 8 and the toothed plate frame 19 are respectively corresponding to the connecting plate 11 and the toothed plate 12. By opening four guide plate slots 18, the toothed plate 12 and the three connecting plates 11 will not come into contact with the inner wall of the seat hole 17 during the lifting and lowering of the upper reaction seat 9, thus avoiding the upper reaction seat 9 and the addition seat 13 being unable to lift and lower within the seat hole 17 due to contact.

[0022] Please see Figure 1 - Figure 4 Specifically Figure 3 The lower outlet of the liquid guide tube 16 is matched with the aperture of multiple receiving tanks 10, and the multiple receiving tanks 10 and the filter membrane are vertically corresponding to the multiple nucleic acid purification columns 5 below. By matching the lower outlet of the liquid guide tube 16 with the aperture of the multiple receiving tanks 10, the sample lysis solution discharged downward through the liquid guide tube 16 will not spill onto the outside of the receiving tanks 10, causing contamination and waste.

[0023] Please see Figure 1 - Figure 4 Specifically Figure 2 The upper end of the liquid guide tube 16 is fixedly connected to the outlet of the quantitative dripping funnel 15. The inner wall of the liquid guide tube 16 is provided with an anti-liquid layer, which is a polytetrafluoroethylene coating. By setting an anti-liquid layer, the polytetrafluoroethylene coating has extremely strong hydrophobicity and chemical stability, and also has properties such as acid and alkali resistance and high temperature resistance. It will not react with reagents such as sample lysis buffer, which can effectively prevent sample lysis buffer from remaining on the inner wall of the liquid guide tube 16. This ensures that the amount of sample lysis buffer added to the container 10 each time is accurate and reduces the detection error caused by sample lysis buffer adhering to the inner wall of the liquid guide tube 16.

[0024] Please see Figure 1 - Figure 4 Specifically Figure 1 A digital display temperature gauge is embedded in the side wall of the detection box 1, and a temperature sensor is embedded in the inner wall of the detection box 1. The temperature sensor is electrically connected to the digital display temperature gauge. By setting up a digital display thermometer and a temperature sensor, the temperature sensor can monitor the temperature inside the detection box 1 in real time. The monitored temperature is transmitted to the digital display thermometer via an electrical connection, and the staff can confirm the real-time temperature inside the detection box 1 by observing the temperature value of the digital display thermometer.

[0025] The working principle of the above embodiment is as follows: When the detection box 1 is in an idle state, the upper reaction seat 9 is parallel to the seat hole 17. At this time, the operator adds the sample to be tested into multiple receiving slots 10. Then, the first brake motor 6 is started, causing its output shaft to drive the drive gear 7 to rotate. The drive gear 7 meshes with the driven gear, and the driven gear meshes with the toothed plate 12, thereby causing the toothed plate 12 to drive the upper reaction seat 9 to descend in the seat hole 17. When the lower end of one of the connecting plates 11 abuts against the bottom of the inner wall of the plate seat 8, Align the seat 13 with the seat hole 17 horizontally, and then use the quantitative dropping funnel 15 to quantitatively inject the sample lysis solution into the lower receiving tank 10 through the liquid guide tube 16. The sample to be tested and the sample lysis solution are lysed in the receiving tank 10 for 5 minutes and impurities are filtered by the filter membrane. The lysed nucleic acid solution can then enter the nucleic acid purification column 5 below. When multiple nucleic acid purification columns 5 contain lysed nucleic acid solutions, the lower reaction seat 3 can be taken out from the detection box 1 through the sampling slot hole 2 and sent to the detection equipment for centralized detection.

[0026] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0027] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An MTHFR gene mutation detection kit, characterized in that: The device includes a detection box (1), a reaction component, and a sampling component. The side wall of the detection box (1) is provided with a sampling slot (2). The sampling component is disposed in the detection box (1) and the sampling slot (2). The upper end of the detection box (1) is provided with a seat hole (17). The reaction component is disposed in the detection box (1) and the seat hole (17). The reaction assembly includes multiple nucleic acid purification columns (5), an upper reaction seat (9), and an additive seat (13). A toothed plate (12) and three connecting plates (11) are fixedly connected to the side wall of the upper reaction seat (9). The lower end of the additive seat (13) overlaps the upper side wall of the toothed plate (12) and the three connecting plates (11). A first brake motor (6) is fixedly installed on the side wall of the detection box (1). A drive gear (7) is fixedly sleeved on the output shaft of the first brake motor (6). The drive gear (7) meshes with a driven gear. The inner wall of the detection box (1) is fixedly installed with a plate seat (8) and a toothed plate frame (19). A through hole is opened on the back of the toothed plate frame (19). The driven gear meshes with the toothed plate (12) at the through hole. A quantitative dripping bucket (15) is fixedly inserted into the addition seat (13). A liquid guide tube (16) is provided on the lower end face of the addition seat (13). Multiple receiving slots (10) are opened at equal intervals on the upper end face of the upper reaction seat (9). Multiple filter membranes corresponding to the receiving slots (10) are embedded in the lower end face of the upper reaction seat (9).

2. The MTHFR gene mutation detection kit according to claim 1, characterized in that: The sampling assembly includes a lower reaction seat (3), which has multiple placement holes. Multiple nucleic acid purification columns (5) are located in the multiple placement holes. The inner walls of the detection box (1) and the sampling slot (2) are provided with a limiting groove. A limiting slide rail (4) is fixedly connected in the limiting groove. The lower end of the upper reaction seat (9) is slidably sleeved on the limiting slide rail (4).

3. The MTHFR gene mutation detection kit according to claim 1, characterized in that: A second brake motor (14) is fixedly installed at the center of the upper reaction seat (9). The output shaft of the second brake motor (14) is fixedly connected to the lower end face of the addition seat (13). A transmission hole is opened on the side wall of the detection box (1). A driven shaft is inserted into the center of the driven gear. The two ends of the driven shaft are rotatably connected to the inner wall of the transmission hole.

4. The MTHFR gene mutation detection kit according to claim 1, characterized in that: The inner wall of the seat hole (17) is provided with guide plate grooves (18) corresponding to the toothed plate (12) and the three connecting plates (11). The side walls of the toothed plate (12) and the three connecting plates (11) are slidably connected to the inner walls of the four guide plate grooves (18). The plate seat (8) and the toothed plate frame (19) are respectively corresponding to the connecting plate (11) and the toothed plate (12).

5. The MTHFR gene mutation detection kit according to claim 1, characterized in that: The lower outlet of the liquid guide tube (16) matches the aperture of the plurality of containment tanks (10), and the plurality of containment tanks (10) and the filter membrane are vertically corresponding to the plurality of nucleic acid purification columns (5) below.

6. The MTHFR gene mutation detection kit according to claim 1, characterized in that: The upper end of the liquid guide tube (16) is fixedly connected to the outlet of the quantitative dripping bucket (15), and the inner wall of the liquid guide tube (16) is provided with an anti-liquid layer, which is a polytetrafluoroethylene coating.

7. The MTHFR gene mutation detection kit according to claim 1, characterized in that: A digital display thermometer is embedded in the side wall of the detection box (1), and a temperature sensor is embedded in the inner wall of the detection box (1). The temperature sensor is electrically connected to the digital display thermometer.

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