A stem cell quality assurance assembly

Abstract

The utility model discloses a kind of stem cell quality inspection components, belong to stem cell quality inspection technical field, to solve the technical problem that stem cell is susceptible to other pollutant interference in the quality inspection process of cell morphology observation under prior art. The stem cell quality inspection component includes negative pressure type super-clean biological workbench and cell morphology observation mechanism, the front of the negative pressure type super-clean biological workbench is provided with first air door and second air door side by side. The stem cell quality inspection component is by using negative pressure type biological super-clean workbench, and the way that microscope is arranged in negative pressure type biological super-clean workbench carries out cell morphology observation inspection to stem cell, can effectively filter the air entering workbench, form clean operating environment, provide reliable guarantee for stem cell quality inspection, greatly reduce the risk of stem cell being contaminated in the process of inspection, improve the accuracy and reliability of inspection result.

Description

Technical Field

[0001] This utility model belongs to the field of stem cell quality inspection technology, specifically relating to a stem cell quality inspection component. Background Technology

[0002] Stem cells, as a special type of cell with self-renewal and differentiation potential, have shown enormous application potential and broad prospects in many fields such as regenerative medicine, disease treatment, and biomedical research. Stem cell quality testing is a crucial step in ensuring the safety and effectiveness of stem cells in clinical applications and scientific research experiments. Among these methods, cell morphology observation is one of the fundamental methods for stem cell quality testing and holds an irreplaceable and important position.

[0003] Cell morphology observation primarily utilizes a microscope to meticulously observe the morphology, size, and nucleocytoplasmic ratio of stem cells, thereby making a preliminary assessment of cell viability and state. During normal growth and differentiation, stem cells exhibit specific patterns and characteristics in their morphology. For example, healthy stem cells typically have a relatively regular shape, clear cell boundaries, and a moderate nucleocytoplasmic ratio. When stem cells are affected by external factors, such as pollution, nutrient deficiency, or an unfavorable microenvironment, their morphology changes significantly. This may manifest as reduced cell size, rounding, blurred cell boundaries, and an abnormally increased or decreased nucleocytoplasmic ratio. These morphological changes are often a direct reflection of decreased stem cell viability or abnormal state. By observing and analyzing these characteristics in a timely manner, researchers can preliminarily assess the quality of stem cells, providing important references for subsequent experimental procedures or clinical applications.

[0004] However, traditional stem cell quality control processes face numerous challenges in cell morphology observation. Specifically, stem cells have extremely stringent requirements for the operating environment, necessitating observation and culture under highly clean conditions to avoid interference from external microorganisms or other contaminants. However, in traditional observation methods, microscopes are typically placed directly on a laboratory bench, often failing to provide such an ideal clean environment. This can easily lead to stem cell contamination during observation, affecting the accuracy of the results and the subsequent use of the stem cells. Therefore, this application proposes a stem cell quality control component. Utility Model Content

[0005] In view of the shortcomings of the prior art, the purpose of this utility model is to provide a stem cell quality testing component, which aims to solve the technical problem that stem cells are easily interfered with by other contaminants during the quality testing process of cell morphology observation under the prior art.

[0006] To address the aforementioned technical problems, this utility model provides a stem cell quality testing component, comprising a negative pressure ultra-clean biological workbench and a cell morphology observation mechanism. The negative pressure ultra-clean biological workbench has a first air vent and a second air vent arranged side-by-side on its front. The first air vent has a pair of first clean operation holes, and the second air vent has a pair of second clean operation holes. Both the first and second clean operation holes are large enough to accommodate the insertion of a laboratory personnel's hand. The cell morphology observation mechanism includes a microscope disposed inside the negative pressure ultra-clean biological workbench facing the first air vent. The microscope eyepiece extends out of the negative pressure ultra-clean biological workbench through the first air vent.

[0007] Preferably, the first clean operation hole and the second clean operation hole are respectively located at the bottom of the first air damper and the second air damper. The first air damper is fixed on the negative pressure ultra-clean biological workbench, and the second air damper is movably disposed on the negative pressure ultra-clean biological workbench and can be pushed and pulled up and down.

[0008] Preferably, gloves are provided on the side of the pair of first clean operating ports and the pair of second clean operating ports facing the negative pressure ultra-clean biological workbench.

[0009] Preferably, the first air damper is provided with a calibration window, which is located above the first clean operation hole. The eyepiece of the microscope passes through the calibration window, and the calibration window is tightly fitted to the eyepiece shell wall of the microscope.

[0010] Preferably, a tray is horizontally arranged inside the negative pressure ultra-clean biological workbench on the side facing the first air vent, the microscope is fixed on the tray, and a dish is vertically fixed on the tray. The dish is located on the side of the microscope facing the second air vent and is used to stack stem cell culture dishes.

[0011] Preferably, the tray is further provided on the tray plate, and the tray is located on the side of the microscope facing the second air vent.

[0012] Preferably, a positioning frame is fixed on the pallet, and the bottom of the pallet is movably nested with the positioning frame.

[0013] Preferably, the top of the petri dish is provided with a ring opening, which is funnel-shaped. The petri dish and the ring opening are vertically provided with an adapter groove on the side facing the first air vent. The bottom of the petri dish is provided with a pickup slot on the side facing the first air vent. The pickup slot can allow the petri dish to be horizontally removed from the petri dish.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows: In this invention, by employing a negative pressure bio-clean bench and placing a microscope inside the negative pressure bio-clean bench to observe and test the cell morphology of stem cells, the air entering the bench can be effectively filtered, creating a clean operating environment. This provides a reliable guarantee for the quality testing of stem cells, greatly reduces the risk of stem cells being contaminated during the testing process, and improves the accuracy and reliability of the testing results.

[0015] In this invention, the design of the first and second air dampers, along with the provision of the first and second clean operating ports, allows laboratory personnel to easily extend their hands into the workbench for operation. The second air damper can be pushed and pulled up and down, further increasing operational flexibility.

[0016] In this invention, the design of the dish and tray provides a stable placement position for the stem cell culture dish. The setting of the adapter slot and pick-up slot makes it easy for the experimenter to pick up and place the culture dish for observation, avoiding the clutter in the negative pressure bio-clean workbench and improving work efficiency. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the cell morphology observation mechanism in this utility model; Figure 3 This is a schematic diagram of the structure of the dish cylinder in this utility model; Figure 4 This utility model Figure 2 Enlarged view of point A in the image.

[0019] The labels in the attached diagram are as follows: 1. Negative pressure ultra-clean biological workbench; 2. First air damper; 3. Second air damper; 4. First clean operation port; 5. Second clean operation port; 6. Inspection window; 7. Microscope; 8. Tray; 9. Dish container; 10. Tray; 11. Pick-up slot; 12. Ring; 13. Adaptor slot; 14. Positioning frame. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] This embodiment provides a stem cell quality testing component, the structural schematic of which is shown below. Figures 1-4 As shown, it includes a negative pressure type bio-clean workbench 1 and a cell morphology observation mechanism.

[0022] In this embodiment, the negative pressure type bio-clean bench 1 serves as the basic platform for the entire assembly, providing a clean operating environment for stem cell quality testing. A first air vent 2 and a second air vent 3 are arranged side-by-side on the front of the negative pressure type bio-clean bench 1. The first air vent 2 is fixedly installed on the negative pressure type bio-clean bench 1; the second air vent 3 is movably installed on the negative pressure type bio-clean bench 1, allowing for vertical sliding. This design facilitates adjustment of airflow and operating space within the bench according to actual needs.

[0023] Furthermore, a pair of first clean operating holes 4 are provided at the bottom of the first air damper 2, and a pair of second clean operating holes 5 are provided at the bottom of the second air damper 3. Both the first clean operating holes 4 and the second clean operating holes 5 are large enough to accommodate the hands of the experimenter, allowing them to operate within the negative pressure bio-clean workbench 1. To ensure cleanliness during operation, gloves are provided on the side of the first clean operating holes 4 and the second clean operating holes 5 facing inwards from the negative pressure bio-clean workbench 1. After inserting their hands into the operating holes, the experimenter uses the gloves to avoid direct contact with the internal environment of the workbench and prevent contamination.

[0024] Furthermore, in this embodiment, the cell morphology observation mechanism includes a microscope 7 installed inside the negative pressure bio-clean bench 1 for observing the morphology of stem cells. Specifically, a support plate 8 is horizontally installed inside the negative pressure bio-clean bench 1 on the side facing the first air vent 2. The microscope 7 is fixed on the support plate 8. The microscope 7 is a commonly available model. The eyepiece of the microscope 7 extends out of the negative pressure bio-clean bench 1 through the calibration window 6 provided on the first air vent 2, facilitating observation by the experimenter. The calibration window 6 is located above the first clean operation hole 4 and is tightly fitted to the eyepiece shell wall of the microscope 7 to ensure airtightness during observation and prevent external contaminants from entering the negative pressure bio-clean bench 1.

[0025] Furthermore, in this embodiment, a dish cylinder 9 is vertically fixed on the tray 8. The dish cylinder 9 is located on the side of the microscope 7 facing the second air vent 3 and is used to stack stem cell culture dishes. A ring opening 12 is provided at the top of the dish cylinder 9. The ring opening 12 has a funnel-shaped structure, which facilitates the placement of culture dishes into the dish cylinder 9 by the experimenter. An adapter groove 13 is vertically provided on the side of the dish cylinder 9 and the ring opening 12 facing the first air vent 2. This adapter groove 13 facilitates the experimenter's observation of the stem cells in the culture dish and also helps to move the culture dish or observe the remaining amount during operation. A pick-up slot 11 is provided at the bottom of the dish cylinder 9 on the side facing the first air vent 2. The experimenter can use the pick-up slot 11 to horizontally remove the culture dish from the dish cylinder 9, making the operation convenient and quick.

[0026] Furthermore, a tray 10 is also provided on the tray 8 for holding the prepared stem cell testing samples. The tray 10 is located on the side of the microscope 7 facing the second air vent 3. A positioning frame 14 is fixed on the tray 8, and the bottom of the tray 10 is movably nested with the positioning frame 14. This design allows the tray 10 to be easily installed and removed. Experimenters can remove the tray 10 for cleaning or replacement as needed. At the same time, the positioning frame 14 can also ensure the stable placement of the tray 10 within the negative pressure bio-clean workbench 1.

[0027] Working principle: This stem cell quality testing component primarily relies on the clean environment provided by the negative pressure bio-clean bench 1 and the stem cell morphology observation function. The negative pressure bio-clean bench 1 creates a negative pressure environment through its internal airflow circulation system, ensuring that outside air, filtered through a high-efficiency filter, enters the bench 1, thus guaranteeing that the air cleanliness within the bench 1 meets the standards required for stem cell quality testing. During operations, personnel place their hands inside gloves within the first clean operating port 4 or the second clean operating port 5, performing various operations within the negative pressure bio-clean bench 1, such as placing and removing stem cell culture dishes. When morphological observation of stem cells is required, the personnel place the culture dish containing the stem cells into the dish container 9. Then, the experimenters picked up the culture dishes containing stem cells one by one from the pick-up slot 11, and prepared samples for observation under the microscope 7 in the negative pressure type biological ultra-clean workbench 1. The prepared observation samples were placed in the tray 10, and then the experimenters observed the prepared stem cell samples through the first clean operation hole 4 with the eyepiece of the microscope 7 to achieve the purpose of quality inspection of stem cells.

[0028] All technical features in this embodiment can be freely combined according to actual needs.

[0029] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A stem cell quality testing component, comprising a negative pressure ultra-clean biological workbench (1) and a cell morphology observation mechanism, characterized in that: The negative pressure ultra-clean biological workbench (1) has a first air door (2) and a second air door (3) arranged side by side on the front. The first air door (2) has a pair of first clean operation holes (4), and the second air door (3) has a pair of second clean operation holes (5). Both the pair of first clean operation holes (4) and the pair of second clean operation holes (5) can accommodate the hands of the experimenter. The cell morphology observation mechanism includes a microscope (7) installed inside the negative pressure ultra-clean biological workbench (1) facing the first air vent (2), with the eyepiece of the microscope (7) extending out of the negative pressure ultra-clean biological workbench (1) through the first air vent (2).

2. The stem cell quality testing component according to claim 1, characterized in that, The first clean operation hole (4) and the second clean operation hole (5) are respectively located at the bottom of the first air door (2) and the second air door (3). The first air door (2) is fixed on the negative pressure ultra-clean biological workbench (1), and the second air door (3) is movably set on the negative pressure ultra-clean biological workbench (1) and can be pushed and pulled up and down.

3. The stem cell quality testing component according to claim 2, characterized in that, Gloves are provided on one side of the first clean operation port (4) and the second clean operation port (5) facing the negative pressure ultra-clean biological workbench (1).

4. The stem cell quality testing component according to claim 2, characterized in that, The first air vent (2) is provided with a calibration window (6), which is located above the first clean operation hole (4). The eyepiece of the microscope (7) passes through the calibration window (6), and the calibration window (6) is tightly fitted to the eyepiece shell wall of the microscope (7).

5. A stem cell quality testing component according to claim 1, characterized in that, The negative pressure ultra-clean biological workbench (1) has a tray (8) horizontally arranged on the side facing the first air vent (2) inside. The microscope (7) is fixed on the tray (8). A dish (9) is vertically fixed on the tray (8). The dish (9) is located on the side of the microscope (7) facing the second air vent (3). The dish (9) is used to stack stem cell culture dishes.

6. A stem cell quality testing component according to claim 5, characterized in that, The tray (8) is also provided with a tray (10), which is located on the side of the microscope (7) facing the second air door (3).

7. A stem cell quality testing component according to claim 6, characterized in that, A positioning frame (14) is fixed on the tray (8), and the bottom of the tray (10) is movably nested with the positioning frame (14).

8. A stem cell quality testing component according to claim 5, characterized in that, The top of the petri dish (9) is provided with a ring opening (12), which is in the shape of a bucket. The petri dish (9) and the ring opening (12) are vertically provided with an adapter groove (13) on the side facing the first air vent (2). The bottom of the petri dish (9) is provided with a pickup slot (11) on the side facing the first air vent (2). The pickup slot (11) can allow the petri dish to be horizontally removed from the petri dish (9).

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