A portable detection device based on isothermal PCR reaction

Abstract

The utility model relates to a portable detection device based on constant temperature PCR reaction, including box, constant temperature heating element, first ladder pipe cylinder, second ladder pipe cylinder, end cover and sealing plug, its inside is provided with colloidal gold test paper in the box, and the box is opened to have the observation window, the box top is opened to have the liquid outlet, and the upper surface of box is provided with the pipe seat, constant temperature heating element sets up in the top surface of box inside, first ladder pipe cylinder lower part is connected with the pipe seat, and the bottom of first ladder pipe cylinder is provided with the reaction bin that passes through up and down, second ladder pipe cylinder lower part is connected with first ladder pipe cylinder, and the bottom of second ladder pipe cylinder is opened to have the liquid inlet, end cover buckles in the top of second ladder pipe cylinder, and end cover is provided with the drip tube, and the bottom end of drip tube is located in the liquid inlet, sealing plug is plugged in the drip port place of drip tube, the device is heated to the reaction bin through constant temperature heating element, makes solution and colloidal gold test paper fast reaction, and shows detection result, and the device is small, convenient to use and fast detection speed.

Description

Technical Field

[0001] This invention belongs to the field of virus detection technology, specifically relating to a portable detection device based on isothermal PCR reaction. Background Technology

[0002] The main method for detecting pathogen DNA is polymerase chain reaction (PCR) technology, which is a technique for amplifying specific DNA fragments in vitro. This technique is widely used in fields such as gene diagnosis and infectious disease monitoring. However, this technique has disadvantages such as large equipment size, inconvenient operation, long amplification reaction time (usually several hours), and difficulty in promoting its application at the grassroots level.

[0003] To improve the portability and speed of HPV virus detection, isothermal PCR is a rapid nucleic acid amplification technology based on ambient temperature. This involves polymerases and other enzymes in the reaction system using specific primers to perform a rapid nucleic acid amplification reaction at a constant temperature. With the assistance of endonucleases, modified specific molecular probes amplify with modified downstream primers to form paired-end modified nucleic acid fragments. As the amplification products accumulate, the final results can be interpreted colorimetrically using nucleic acid test strips or fluorescently using a small fluorescence detector.

[0004] Therefore, there is an urgent need to develop a virus detection device that is small in size, fast in response, and easy to operate. Utility Model Content

[0005] To address at least one of the problems in the prior art, the purpose of this invention is to provide a portable detection device based on isothermal PCR reaction. By heating the reaction chamber with an isothermal heating element, the solution inside can react rapidly with colloidal gold test paper, and the detection results can be displayed. The device is small in size, easy to use, and has a fast detection speed.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A portable detection device based on isothermal PCR reaction, comprising: The box contains colloidal gold test strips and has an observation window located above the colloidal gold test strips. The top of the box has a liquid outlet, and the upper surface of the box has a tube seat with the liquid outlet located inside the tube seat. A constant temperature heating element is disposed on the top surface inside the box body and located below the tube base; The first stepped tube is connected to the tube base at its lower part, and a reaction chamber that runs vertically through the bottom surface of the first stepped tube is provided. The lower part of the second stepped tube is connected to the first stepped tube, and the bottom surface of the second stepped tube is provided with a liquid inlet. An end cap is fastened to the top of the second stepped tube, and the end cap is provided with a drip tube, the bottom end of which is located inside the inlet. A sealing plug is used to seal the drip outlet of the drip tube.

[0007] Preferably, a TYPE-C interface is provided on one side of the housing, and the TYPE-C interface is connected to the constant temperature heating element.

[0008] Preferably, the box body includes an upper box body and a lower box body that are connected to each other.

[0009] Preferably, the side wall of the tube seat is provided with a first slide rail, and the first stepped tube is provided with a cylindrical first slider, which can slide along the first slide rail.

[0010] Preferably, the first slide rail is provided with a first limiting boss at each end to limit the position of the first slider within the first slide rail.

[0011] Preferably, the side wall of the first stepped tube is provided with a second slide rail, and the second stepped tube is provided with a cylindrical second slider, which can slide along the second slide rail.

[0012] Preferably, the second slide rail is provided with a second limiting boss at each end to limit the position of the second slider within the second slide rail.

[0013] Preferably, the observation window is provided with a transparent plastic film.

[0014] Preferably, the inner wall of the reaction chamber is configured as a toothed rack.

[0015] Preferably, the bottom surface of the second stepped tube is provided with a sealing boss, which is used to cover the top of the reaction chamber after the second stepped tube is rotated.

[0016] This utility model has the following advantages due to the adoption of the above technical solution: 1. The portable detection device based on isothermal PCR reaction provided by this utility model can heat the reaction chamber through an isothermal heating element. It can be powered by a power bank through a TYPE-C interface, enabling the solution inside to react quickly with colloidal gold test paper and display the test results. The device is small in size, easy to use and fast in detection, suitable for rapid on-site detection, and adaptable to the needs of primary medical institutions and on-site testing. 2. The portable detection device based on isothermal PCR reaction provided by this utility model achieves the sealing of the reaction chamber through a double-layer rotating mechanism. The ingenious structural design can avoid the leakage and diffusion of aerosols during the reaction process. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a portable detection device based on isothermal PCR reaction provided in one embodiment of the present invention.

[0018] Figure 2 This is an exploded schematic diagram of the portable detection device based on isothermal PCR reaction provided in this embodiment of the present invention.

[0019] Figure 3 This is a longitudinal cross-sectional schematic diagram of the portable detection device based on isothermal PCR reaction provided in this embodiment of the present invention.

[0020] Figure 4 This is a schematic diagram of the upper box body provided in this embodiment of the present invention.

[0021] Figure 5 This is a schematic diagram of the lower box body provided in this embodiment of the present invention.

[0022] Figure 6 This is a top view schematic diagram of the first stepped tube provided in this embodiment of the present invention.

[0023] Figure 7 This is a bottom view of the first stepped tube provided in this embodiment of the present invention.

[0024] Figure 8 This is a top view schematic diagram of the second stepped tube provided in this embodiment of the present invention.

[0025] Figure 9 This is a bottom view of the second-step tube provided in this embodiment of the present invention.

[0026] Marked in the attached diagram: 1 is the box body, 101 is the upper box body, 102 is the lower box body, 2 is the constant temperature heating element, 3 is the first stepped tube, 301 is the first slider, 302 is the second slide rail, 303 is the second limiting boss, 4 is the second stepped tube, 401 is the second slider, 402 is the sealing boss, 5 is the end cap, 501 is the drip tube, 6 is the sealing plug, 7 is the colloidal gold test paper, 8 is the observation window, 9 is the liquid outlet, 10 is the tube seat, 1001 is the first slide rail, 1002 is the first limiting boss, 11 is the reaction chamber, 12 is the TYPE-C interface, and 13 is the colloidal gold test paper placement platform. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0028] In the description of this utility model, it should be noted that the terms "upper", "lower", "front", "rear", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the system or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0029] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "assembly," "setup," and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0030] This invention provides a portable detection device based on isothermal PCR reaction. The reaction chamber is heated by an isothermal heating element, which enables the solution inside to react rapidly with colloidal gold test paper and display the detection results. The device is small in size, easy to use, and has a fast detection speed. The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0031] Example 1 Please refer to Figures 1 to 3 This embodiment provides a portable detection device based on isothermal PCR reaction, including a box body 1, an isothermal heating element 2, a first-step tube 3, a second-step tube 4, an end cap 5, and a sealing plug 6. The box 1 contains colloidal gold test strips 7 and has an observation window 8 located above the colloidal gold test strips 7. The top of the box 1 has an outlet 9 and a tube seat 10 is provided on the upper surface of the box 1, with the outlet 9 located inside the tube seat 10. The constant temperature heating element 2 is disposed on the top surface inside the box 1 and is located below the tube seat 10; The lower part of the first-step tube 3 is connected to the tube seat 10, and the bottom surface of the first-step tube 3 is provided with a reaction chamber 11 that runs vertically through the tube. The lower part of the second-step tube 4 is connected to the first-step tube 3, and the bottom surface of the second-step tube 4 is provided with a liquid inlet. The end cap 5 is fastened to the top of the second-step tube 4, and the end cap 5 is provided with a drip tube 501, the bottom end of which is located inside the inlet. The sealing plug 6 is placed at the drip outlet of the drip tube 501.

[0032] Specifically, the entire housing of the detection device, including the box 1, the first-step tube 3, the second-step tube 4, and the tube base 10, is made of thermoplastic ABS. ABS has high strength and good mechanical and thermal properties, ensuring that the housing does not deform at 70°C during the isothermal PCR reaction. ABS's good thermal conductivity allows the enzyme to react fully within the reaction chamber 11.

[0033] In this embodiment, the box body 1 is a square box body, and a TYPE-C interface 12 is provided on one side of the box body 1. The TYPE-C interface 12 is connected to the constant temperature heating element 2.

[0034] Specifically, the constant temperature heating element 2 uses a ceramic resistance heating element found in existing technologies, such as an electric heating wire core constant temperature heating element, which can achieve a constant output temperature of 70°C under a 5V working voltage. Utilizing the thermal conductivity of ABS material, the temperature of the reaction chamber 11 is maintained above 60°C but will not exceed 70°C, ensuring the normal occurrence of the enzyme reaction. The constant temperature heating element 2 is powered via a TYPE-C interface 12, which can be powered by a power bank or a mobile phone charger. The heating time is 15-20 minutes.

[0035] Please refer to Figures 4 to 5 In this embodiment, the box body 1 includes an upper box body 101 and a lower box body 102 that are connected to each other.

[0036] Specifically, the upper box 101 and the lower box 102 are connected by a snap-fit ​​or screws. An observation window 8 is located on the upper box 101, and a transparent plastic film is placed on the window 8 to facilitate observation of the results displayed by the colloidal gold test strip 7, while also providing a seal. The colloidal gold test strip 7 is pre-placed inside the box 1. A colloidal gold test strip placement platform 13 is located at the bottom of the lower box 102. The platform 13 has a slot, and the colloidal gold test strip 7 is placed within this slot. The platform 13 supports the colloidal gold test strip 7 and prevents it from shifting within the box 1, thus ensuring accurate observation of the measurement results.

[0037] In this embodiment, a first slide rail 1001 is provided on the side wall of the tube base 10, and a cylindrical first slider 301 is provided on the first stepped tube 3. The first slider 301 can slide along the first slide rail.

[0038] Specifically, the tube seat 10 and the upper box body 101 are integrally formed. The tube seat 10 is a circular tube, and its bottom surface is the upper surface of the upper box body 101. The liquid outlet 9 is located at a non-center position on the bottom surface of the tube seat 10. The liquid outlet 9 can be a square opening with a chamfered top edge to facilitate the flow of liquid into the liquid outlet 9. The bottom of the liquid outlet 9 extends into the box body 1 to facilitate the flow of liquid through the liquid outlet 9 onto the colloidal gold test paper 7. The colloidal gold test paper 7 is located below the liquid outlet 9.

[0039] Two symmetrically arranged first slide rails 1001 are provided on the side wall of the tube seat 10. First limiting bosses 1002 are provided at both ends of the first slide rails 1001. The first limiting bosses 1002 and the end faces of the first slide rails 1001 form a limiting groove. When the first slider 301 is engaged in the limiting groove, its position within the first slide rail 1001 is restricted. When the first slide rail 1001 is located in the limiting groove at one end, the reaction chamber 11 is aligned with the liquid outlet 9. When the first slide rail 1001 is located in the limiting groove at the other end, the bottom surface of the reaction chamber 11 is in sealed contact with the bottom surface of the tube seat 10, ensuring that the contents of the reaction chamber 11 do not flow from its bottom end. Furthermore, the reaction chamber 11 is located directly above the constant temperature heating element 2, allowing for better heating of the reaction chamber 11.

[0040] The first stepped tube 3 is a stepped cylinder, and two first sliders 301 are symmetrically arranged, each located within its respective first slide rail 1001. Rotating the first stepped tube 3 allows the two limiting grooves of the first slider 301 within the first slide rail 1001 to switch.

[0041] Please refer to Figures 6 to 7 In this embodiment, the inner wall of the reaction chamber 11 is configured as a toothed rack.

[0042] Specifically, the reaction chamber 11 is located inside the first-step tube 3, with openings at both its top and bottom. The inner wall of the reaction chamber 11 is uniformly arranged in a rack-like shape, similar to an internal gear structure. The reaction chamber 11 is pre-filled with lyophilized amplification enzyme microspheres. The rack-like structure prevents the lyophilized amplification enzyme microspheres from moving, ensuring that the dropped sample does not contact the microspheres. Both the top and bottom of the reaction chamber 11 are provided with annular grooves, each containing a sealing ring. These sealing rings maintain a seal between the reaction chamber 11 and the bottom surface of the tube base 10 and the second-step tube 4.

[0043] Please refer to Figures 8 to 9 In this embodiment, a second slide rail 302 is provided on the side wall of the first stepped tube 3, and a cylindrical second slider 401 is provided on the second stepped tube 4. The second slider 401 can slide along the second slide rail 302. Second limiting bosses 303 are provided at both ends of the second slide rail 302 to limit the position of the second slider 401 within the second slide rail 302.

[0044] Specifically, two symmetrically arranged second slide rails 302 are provided on the side wall of the first stepped tube 3. Second limiting bosses 303 are provided at both ends of the second slide rails 302. The second limiting bosses 303 and the end faces of the second slide rails 302 form a limiting groove. When the second slider 401 is engaged in the limiting groove, its position within the second slide rail 302 is restricted. When the second slider 401 is located in one of the limiting grooves, the reaction chamber 11 is aligned with the dripping pipe 501. A sealing boss 402 is provided on the bottom surface of the second stepped tube 4. Rotating the second stepped tube 4 covers the top of the reaction chamber. When the second slider 401 is located in the limiting groove at the other end, the top surface of the reaction chamber 11 contacts the sealing boss 402 of the second stepped tube 4, ensuring a tight seal between the reaction chamber 11 and the bottom surface of the second stepped tube 4.

[0045] The second-step tube 4 is also a stepped cylinder. The end cap 5 is fastened to the top of the second-step tube 4. The space formed by the end cap 5 and the second-step tube 4 is a buffer zone, which can prevent pathogen aerosols from spreading to the external environment. The drip tube 501 of the end cap 5 is tubular, and its bottom end is directly inserted into the liquid inlet.

[0046] In this embodiment, the sealing plug 6 is a rubber plug with a connector. The end cap 5 has a connection hole, and the connector is snapped into the connection hole, which can prevent the sealing plug 6 from being lost after being removed from the drip tube 501.

[0047] When using the portable detection device based on isothermal PCR reaction of this embodiment for HPV virus detection, the device is a single-use device. The reaction chamber 11 is pre-filled with lyophilized microspheres of amplifying enzyme. The bottom of the reaction chamber 11 is pre-sealed to the tube seat 10, and the top of the reaction chamber 11 is aligned with the dropper 501. The sealing plug 6 is removed, and two to three drops (approximately 40 μL) of sample are added through the dropper 501. After waiting 10 seconds, the sealing plug 6 is reattached. At this point, the sample has entered the reaction chamber 11, and the lyophilized microspheres of amplifying enzyme dissolve within it. The reaction chamber 11 has a space of approximately 0.5 ml. After the sample and enzyme are mixed, the second-step tube 4 is rotated to seal the top of the reaction chamber 11, making it a closed chamber. The isothermal heating element 2 is energized to heat the reaction chamber 11 for 15 to 20 minutes to ensure the normal occurrence of the enzyme reaction. Rotate the second-step tube 4 to align the dropper 501 with the reaction chamber 11, remove the sealing plug 6, and inject the diluent into the reaction chamber 11. The diluent is approximately 50 times the volume of the sample, about 2 ml. After injecting the diluent, replace the sealing plug 6 to prevent aerosols containing pathogens from spreading out of the shell. Simultaneously, rotate the first-step tube 3 to align the bottom of the reaction chamber 11 with the outlet 9. The diluted sample is absorbed by the colloidal gold test paper 7 through the outlet 9. The test is completed through the absorbent layer of the colloidal gold test paper 7, and the test results are displayed. The tester can observe the test results through the observation window 8.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A portable detection device based on isothermal PCR reaction, characterized in that, include: The box contains colloidal gold test strips and has an observation window located above the colloidal gold test strips. The top of the box has a liquid outlet, and the upper surface of the box has a tube seat with the liquid outlet located inside the tube seat. A constant temperature heating element is disposed on the top surface inside the box body and located below the tube seat; The first stepped tube is connected to the tube seat at its lower part, and a reaction chamber that runs vertically through the bottom surface of the first stepped tube is provided. The second stepped tube is connected to the first stepped tube at its lower part, and the bottom surface of the second stepped tube has a liquid inlet. An end cap is fastened to the top of the second stepped tube, and the end cap is provided with a drip tube, the bottom end of which is located inside the inlet. A sealing plug is used to seal the drip outlet of the drip tube.

2. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, A TYPE-C interface is provided on one side of the box, and the TYPE-C interface is connected to the constant temperature heating element.

3. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, The box body includes an upper box body and a lower box body that are connected to each other.

4. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, The side wall of the tube seat is provided with a first slide rail, and the first stepped tube is provided with a cylindrical first slider, which can slide along the first slide rail.

5. The portable detection device based on isothermal PCR reaction according to claim 4, characterized in that, The first slide rail is provided with a first limiting boss at each end, which is used to limit the position of the first slider within the first slide rail.

6. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, The first stepped tube has a second slide rail on its side wall, and the second stepped tube has a cylindrical second slider that can slide along the second slide rail.

7. The portable detection device based on isothermal PCR reaction according to claim 6, characterized in that, The second slide rail is provided with a second limiting boss at each end to limit the position of the second slider within the second slide rail.

8. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, A transparent plastic film is provided on the observation window.

9. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, The inner wall of the reaction chamber is designed with a toothed shape.

10. The portable detection device based on isothermal PCR reaction according to claim 1, characterized in that, The bottom surface of the second stepped tube is provided with a sealing boss, which is used to cover the top of the reaction chamber after the second stepped tube is rotated.

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