A petri dish stimulating electrode plate and cell electric stimulation device

Abstract

This utility model relates to the field of cell stimulation technology and discloses a culture dish stimulation electrode plate and a cell electrical stimulation device, comprising: a circuit board, a solder pad on the upper surface of the circuit board, a solder pad hole on the upper surface of the solder pad, and a gold-plated plate on the inner wall of the solder pad hole; guide rails on both sides of the solder pad, and a conductive block installed at the center of the upper surface of the guide rail; a slider slidably connected to the upper surface of the guide rail, and an electrode installed at the center of the upper surface of the slider; clamping blocks on both sides of the inner wall of the slider, and a lead screw threadedly connected to both sides of the slider, with the inner end of the lead screw connected to the front of the corresponding clamping block; and ball bearings rotatably connected to both sides of the lower surface of the slider, with the surface of the ball bearings slidingly fitting against the surface of the circuit board. This electrode plate allows for flexible adjustment and replacement of electrodes, solving the problem of inconvenient replacement of traditional fixed electrode plates. The ball bearings reduce sliding resistance, and the lead screw and clamping blocks cooperate to achieve stable fixation of the slider.

Description

Technical Field

[0001] This utility model relates to the field of cell stimulation technology, specifically to a culture dish stimulation electrode plate and a cell electrical stimulation device. Background Technology

[0002] Cellular electrical stimulation technology is widely used in medical research, cell proliferation and differentiation regulation, tissue growth promotion and wound healing mechanism exploration. As the core component of this technology system for achieving precise cell electrical stimulation, the structure design of the culture dish stimulation electrode plate is directly related to the adaptability, ease of operation and stability of the results. It provides a basic support for studying the effects of electrical signals on cell migration, adhesion and metabolism. Therefore, its performance is crucial to the efficiency and reliability of cell electrical stimulation experiments.

[0003] Existing culture dish stimulation electrode plates suffer from several structural design flaws, making them unsuitable for experimental needs. Firstly, current technology typically employs a design where electrode components are directly fixed to a circuit board or support substrate. The distance between electrodes is predetermined during manufacturing and cannot be adjusted based on experimental requirements. Since the electric field strength of cell electrostimulation is inversely proportional to the distance between electrodes, and different cell types have significantly different requirements for effective electric field strength, fixed-spaced electrode plates can only accommodate the stimulation conditions of a single cell type. When the experimental subjects are changed to other cell types, a dedicated electrode plate with the corresponding spacing must be replaced, limiting the applicability of the electrode plate and increasing the cost of experimental consumables and the complexity of electrode plate replacement. Secondly, observation convenience is insufficient. Traditional electrode plates lack dedicated transparent observation structures between adjacent pads. When observing cell status using a microscope, the electrode plate's own structure easily obstructs the view, making it difficult to clearly and in real-time monitor cell changes during electrostimulation, thus hindering experimental observation. Therefore, this paper proposes a culture dish stimulation electrode plate and a cell electrostimulation device. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a culture dish stimulation electrode plate and a cell electrical stimulation device to solve the aforementioned technical problems that limit the application scenarios of the electrode plate.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a culture dish stimulation electrode plate and a cell electrical stimulation device, comprising:

[0006] The circuit board, and the pads and waterproof interfaces provided on the upper surface of the circuit board, wherein the upper surface of the pads is provided with pad holes, and a gold-plated plate is provided on the inner wall of the pad holes. The gold-plated plate extends downward through the upper surface of the circuit board, and a transparent lens is provided on the upper surface of the circuit board. The transparent lens is provided between adjacent pads, and limit holes are provided around the upper surface of the circuit board.

[0007] The guide rail is located on both sides of the pad and is connected to the upper surface of the circuit board. A conductive block is installed at the center of the upper surface of the guide rail and is in contact with the gold-plated plate.

[0008] The slider is slidably connected to the center of the upper surface of the guide rail, and a gold-plated positioning hole is opened at the center of the upper surface of the slider. An electrode is added to the inner cavity of the gold-plated positioning hole, and the electrode is in contact with the corresponding part of the conductive block.

[0009] Clamping blocks are disposed on both sides of the inner wall of the slider, and lead screws are threadedly connected to both sides of the slider. The inner end of the lead screw is connected to the front of the corresponding clamping block, and the inner side of the clamping block is tightly fitted with the surface of the guide rail. Ball bearings are rotatably connected to both sides of the lower surface of the slider, and the surface of the ball bearings slides in contact with the surface of the circuit board.

[0010] The culture plate is placed below the circuit board, and the bottom end of the gold plate extends into the culture well of the culture plate, so that the gold plate comes into contact with the cells in the culture well. At the same time, the material of the gold plate meets the requirements for cell electrical stimulation. The slider is pushed to slide along the center of the upper surface of the guide rail, and the ball bearings on both sides of the lower surface of the slider slide and fit against the surface of the circuit board, reducing the frictional resistance between the slider and the circuit board.

[0011] Rotating the lead screw causes the clamping block to fit tightly against the surface of the guide rail, thus fixing the slider on the guide rail and preventing the slider from shifting during the experiment and causing changes in the electrode distance.

[0012] Place the electrode into the gold-plated positioning hole in the center of the upper surface of the slider, adjust the position of the electrode to ensure that the corresponding part of the electrode and the conductive block are in close contact, and complete the stable connection between the electrode and the conductive path.

[0013] Connect the waterproof interface on the circuit board to the external cell electrostimulator so that the stimulation signal generated by the external cell electrostimulator can be transmitted to the circuit board and electrodes through the waterproof interface.

[0014] When the external cell electrical stimulator is activated, the electrodes deliver stimulation signals to the target cells through the conductive block and the gold-plated plate. Simultaneously, the growth and morphological changes of the cells during the electrical stimulation process can be directly observed through a transparent lens placed between adjacent pads on the upper surface of the circuit board.

[0015] Preferably, the upper surface of the circuit board has an annular groove, the position of which corresponds to the position of the transparent lens, and an inner annular groove is formed at the center of the inner wall of the annular groove. This provides a precise installation and positioning reference for the transparent lens and its subsequent supporting components, avoiding deviations in the installation position that could cause the observation channel to shift or become blocked, and ensuring the stable realization of the core through-viewing function of the observation window. The inner annular groove also provides space for the subsequent rotation and adjustment of the transparent lens without requiring significant modifications to the original structure of the circuit board. This maintains the structural stability of the circuit board and lays a structural foundation for expanding the observation dimensions.

[0016] Preferably, an annular frame is added to the outer surface of the transparent lens, and sealing rings are installed on the upper and lower parts of the outer side of the annular frame, with the surface of the sealing rings tightly fitted to the inner wall of the annular groove. The annular frame can provide effective structural support and protection for the transparent lens, preventing the transparent lens from being damaged by direct collision and friction with other structures during assembly or adjustment, extending the service life of the transparent lens, and maintaining the structural flatness of the transparent lens to ensure light transmission stability. The tight fit between the sealing ring and the inner wall of the annular groove can significantly improve the assembly sealing between the transparent lens assembly and the circuit board, effectively preventing impurities or moisture in the cell culture environment from entering the installation gap. This not only meets the clean environment requirements of cell culture but also prevents impurities from adhering to the transparent lens or the annular groove, affecting the clarity of observation.

[0017] Preferably, the transparent lens is rotatably connected to the center of both sides by side posts, and the outer surface of the side posts is rotatably fitted with the inner wall of the inner ring groove. This breaks through the design limitation of existing fixed circular hole observation windows that can only be observed vertically. Through the rotational cooperation between the side posts and the inner ring groove, multi-angle adjustment of the transparent lens can be achieved, which can adapt to the needs of observing the three-dimensionality of cells under oblique light of a microscope and tracking cell migration paths from multiple angles, significantly expanding the observation dimensions.

[0018] Preferably, the fixed frame plate is located below the circuit board, and limit posts are installed around the upper surface of the fixed frame plate. The limit posts are inserted into limit holes, and a fixed plate is installed at the center of the top of the limit posts. A screw is rotatably connected to the center of the upper surface of the fixed plate, and an external connecting shank is installed at the top of the screw. A sliding plate is fitted on the upper surface of the screw, and an upper pressure plate and a lower pressure plate are rotatably connected to the outside of the fixed plate. The corresponding parts of the upper pressure plate and the lower pressure plate are rotatably connected.

[0019] Place the fixing frame plate under the circuit board, align the limiting post with the limiting hole, and then insert the limiting post into the limiting hole. By connecting the limiting post with the limiting hole, the initial positioning of the fixing frame plate and the circuit board is completed, so as to avoid relative displacement between the two in subsequent operations.

[0020] The external connecting handle drives the screw to rotate around the center of the upper surface of the fixed plate, where the fixed plate is installed at the top center of the limit post, providing a stable rotation support base for the screw;

[0021] As the screw rotates, the slide plate, which is fitted onto the upper part of the screw surface, moves along the axial direction of the screw. Since the upper pressure plate is rotatably connected to the outside of the slide plate and the lower pressure plate is rotatably connected to the outside of the fixed plate, and the corresponding parts of the upper and lower pressure plates are rotatably connected, the movement of the slide plate will synchronously drive the upper and lower pressure plates to rotate around their own rotational connection points with the slide plate and the fixed plate, respectively. Continue to rotate the outer connecting handle until the upper and lower pressure plates are in contact with the surface of the circuit board, forming a stable clamp, and completing the circuit board fixing process.

[0022] Preferably, a groove is formed at the center of the outer side of the slide plate and the fixing plate, and a rotating shaft is rotatably connected to both sides of the inner wall of the groove of the slide plate and the fixing plate. By forming a groove at the center of the outer side of the slide plate and the fixing plate, a precise installation and positioning space can be provided for the rotating shaft, avoiding positional displacement of the rotating shaft during assembly and ensuring the accuracy of subsequent component connections.

[0023] Preferably, the end of the rotating shaft inside the groove on the sliding plate and the fixed plate is connected to the corresponding sides of the upper and lower pressure plates. This connection method directly establishes the transmission relationship between the rotating shaft and the upper and lower pressure plates, enabling the rotation of the rotating shaft to be directly transmitted to the upper and lower pressure plates, achieving synchronous motion linkage among the three and avoiding delays or failures in the motion transmission process. The design of connecting the end of the rotating shaft to the corresponding sides of the upper and lower pressure plates allows the upper and lower pressure plates to bear the load evenly when subjected to force, preventing deformation or damage to the upper or lower pressure plates due to excessive local force, and extending the service life of the components.

[0024] Preferably, a rotating rod is provided between the corresponding positions of the upper and lower pressure plates, with the upper surface of the rotating rod connected to the lower surface of the upper pressure plate, and both ends of the rotating rod rotatably connected to the upper sides of the lower pressure plate. The addition of the rotating rod further enhances the linkage stability between the upper and lower pressure plates, preventing misalignment or separation during movement and ensuring consistency in their movement directions. The structure, with the upper surface of the rotating rod fixedly connected to the lower surface of the upper pressure plate and both ends rotatably connected to the upper sides of the lower pressure plate, effectively transmits the movement of the upper pressure plate to the rotating rod and allows for flexible adjustment of the relative angle between the upper and lower pressure plates through the rotation of the rotating rod. This improves the overall structural flexibility, adapts to different operating conditions, and reduces direct frictional loss between the upper and lower pressure plates.

[0025] Compared with the prior art, this utility model provides a culture dish stimulation electrode plate and a cell electrical stimulation device, which has the following beneficial effects:

[0026] This culture dish stimulation electrode plate and cell electrostimulation device uses linear slide rails and matching electrode-carrying sliders set on both sides of the circuit board pad holes. The sliders can slide flexibly along the slide rails to adjust the distance between the electrodes. Since the electric field strength is inversely proportional to the distance between the electrodes, it meets the stimulation distance requirements of different cells and eliminates the need to replace fixed electrodes of different specifications. At the same time, the sliders can slide and separate from the slide rails, which facilitates the replacement of electrodes and solves the problem of the limited application scenarios of traditional fixed-distance electrode plates.

[0027] The lower surface of the slider is equipped with ball bearings, which effectively reduces sliding resistance and facilitates quick adjustment of the electrode distance by the experimenter. The slider is connected to the clamping blocks on both sides by lead screws. After adjustment, tightening the lead screws will make the clamping blocks fit tightly against the guide rail, so as to achieve stable fixation of the slider and prevent electrode displacement during electrical stimulation. The transparent lens between adjacent pads facilitates operation and observation. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0029] Figure 2 This is a schematic diagram of the separation structure of the circuit board and the fixed frame plate of this utility model;

[0030] Figure 3 This is a schematic diagram of the limiting post and its connection structure of the present invention;

[0031] Figure 4 This is a schematic diagram of the solder pads and their connection structure of this utility model;

[0032] Figure 5 This is a schematic cross-sectional view of the upper part of the slider of this utility model;

[0033] Figure 6 This is a schematic diagram of the separation structure of the annular frame and the inner ring groove of this utility model.

[0034] In the diagram: 1. Circuit board; 2. Annular groove; 3. Inner ring groove; 4. Annular frame; 5. Side connecting post; 6. Sealing ring; 7. Transparent lens; 8. Solder pad; 9. Solder pad hole; 10. Guide rail; 11. Conductive block; 12. Slider; 13. Gold-plated positioning hole; 14. Electrode; 15. Clamping block; 16. Lead screw; 17. Ball bearing; 18. Limiting hole; 19. Fixing frame plate; 20. Limiting post; 21. Fixing plate; 22. Screw; 23. Outer connecting handle; 24. Slide plate; 25. Upper pressure plate; 26. Lower pressure plate. Detailed Implementation

[0035] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0036] This utility model provides a technical solution, a culture dish stimulation electrode plate and a cell electrical stimulation device, including: Please refer to Figure 1 , Figure 4 , Figure 5 and Figure 6 The circuit board 1, and the pads 8 and waterproof interfaces disposed on the upper surface of the circuit board 1, wherein the upper surface of the pads 8 is provided with pad holes 9, and a gold-plated plate is provided on the inner wall of the pad holes 9. The gold-plated plate extends downward through the upper surface of the circuit board 1, and a transparent lens 7 is provided on the upper surface of the circuit board 1. The transparent lens 7 is disposed between adjacent pads 8, and limit holes 18 are provided around the upper surface of the circuit board 1.

[0037] The guide rail 10 is disposed on both sides of the pad 8 and is connected to the upper surface of the circuit board 1. A conductive block 11 is installed at the center of the upper surface of the guide rail 10 and is in contact with the gold plate.

[0038] The slider 12 is slidably connected to the center of the upper surface of the guide rail 10, and a gold-plated positioning hole 13 is provided at the center of the upper surface of the slider 12. An electrode 14 is provided in the inner cavity of the gold-plated positioning hole 13, and the electrode 14 is in contact with the corresponding part of the conductive block 11.

[0039] Clamping blocks 15 are disposed on both sides of the inner wall of slider 12, and screws 16 are threadedly connected to both sides of slider 12. The inner end of screw 16 is connected to the front of the corresponding clamping block 15, and the inner side of clamping block 15 is tightly fitted to the surface of guide rail 10. Ball bearings 17 are rotatably connected to both sides of the lower surface of slider 12, and the surface of ball bearings 17 slides and fits against the surface of circuit board 1.

[0040] The culture plate is placed below the circuit board 1, and the bottom end of the gold plate extends into the culture well of the culture plate, so that the gold plate comes into contact with the cells in the culture well. At the same time, the material of the gold plate meets the requirements for cell electrical stimulation. The slider 12 is pushed to slide along the center of the upper surface of the guide rail 10. The ball bearings 17 on both sides of the lower surface of the slider 12 slide and fit against the surface of the circuit board 1, reducing the frictional resistance between the slider 12 and the circuit board 1, until the slider 12 moves to the position of the electrode 14 that meets the current experimental cell or culture dish specifications.

[0041] Rotating the lead screw 16 causes the clamping block 15 to fit tightly against the surface of the guide rail 10, thereby fixing the slider 12 on the guide rail 10 and preventing the slider 12 from shifting during the experiment, which would cause a change in the distance of the electrode 14.

[0042] Place the electrode 14 into the inner cavity of the gold-plated positioning hole 13 opened in the center of the upper surface of the slider 12, adjust the position of the electrode 14 to ensure that the electrode 14 and the corresponding part of the conductive block 11 are tightly attached, and complete the stable connection between the electrode 14 and the conductive path.

[0043] Connect the waterproof interface on the circuit board 1 to the external cell electrical stimulator so that the stimulation signal generated by the external cell electrical stimulator can be transmitted to the circuit board 1 and the electrode 14 through the waterproof interface.

[0044] When the external cell electrostimulator is activated, the electrode 14 applies the stimulation signal to the target cell through the conductive block 11 and the gold plate. At the same time, the growth and morphological changes of the cell during the electrostimulation process can be directly observed through the transparent lens 7 set between the adjacent pads 8 on the upper surface of the circuit board 1.

[0045] Through the sliding engagement of the guide rail 10 and the slider 12, combined with the locking structure of the lead screw 16 and the clamping block 15, the distance of the electrode 14 can be flexibly adjusted and the electrode 14 can be easily replaced.

[0046] Please see Figure 6 An annular groove 2 is formed on the upper surface of the circuit board 1, and the position of the annular groove 2 corresponds to the position of the transparent lens 7. An inner annular groove 3 is formed at the center of the inner wall of the annular groove 2. The annular groove 2 is machined on the upper surface of the circuit board 1 to ensure that the position of the annular groove 2 corresponds precisely to the position of the transparent lens 7. After the annular groove 2 is formed, the inner annular groove 3 is further machined at the center of the inner wall of the annular groove 2, which completes the preparation of the mounting structure on the circuit board 1 for fitting the related components of the transparent lens 7. This provides a precise mounting and positioning reference for the transparent lens 7 and its subsequent supporting components, avoiding the displacement or blockage of the observation channel due to installation position deviation, and ensuring the stable realization of the core through-viewing function of the observation window. The inner annular groove 3 provides adaptation space for the subsequent rotation and adjustment of the transparent lens 7 without the need for major modification of the original structure of the circuit board 1. This not only preserves the structural stability of the circuit board 1, but also lays a structural foundation for expanding the observation dimensions.

[0047] An annular frame 4 is added to the outer surface of the transparent lens 7, and sealing rings 6 are installed on the upper and lower parts of the outer side of the annular frame 4, with the surface of the sealing ring 6 tightly fitting against the inner wall of the annular groove 2. The transparent lens 7 assembly with the annular frame 4 and sealing ring 6 is installed into the annular groove 2 of the circuit board 1, so that the surface of the sealing ring 6 is completely and tightly fitted against the inner wall of the annular groove 2, completing the initial assembly of the transparent lens 7 assembly and the circuit board 1. The annular frame 4 can provide effective structural support and protection for the transparent lens 7, preventing the transparent lens 7 from being damaged by direct collision and friction with other structures during assembly or adjustment, extending the service life of the transparent lens 7, while maintaining the structural flatness of the transparent lens 7 and ensuring light transmission stability. The tight fit between the sealing ring 6 and the inner wall of the annular groove 2 can significantly improve the assembly sealing between the transparent lens 7 assembly and the circuit board 1, effectively preventing impurities or moisture in the cell culture environment from entering the installation gap, which not only meets the clean environment requirements of cell culture, but also prevents impurities from adhering to the transparent lens 7 or the annular groove 2 and affecting the clarity of observation.

[0048] The transparent lens 7 is rotatably connected to the center of both sides by side connecting posts 5, and the outer surface of the side connecting posts 5 rotates and fits against the inner wall of the inner ring groove 3. After assembly, by driving the transparent lens 7 with external force, the transparent lens 7 can drive the side connecting posts 5 to rotate within the inner ring groove 3, thereby achieving angle adjustment of the transparent lens 7. After adjustment, the cooperation between the side connecting posts 5 and the inner ring groove 3 can maintain the stable position of the transparent lens 7. This breaks through the design limitation of existing fixed circular hole observation windows that can only be observed vertically. Through the rotational cooperation between the side connecting posts 5 and the inner ring groove 3, multi-angle adjustment of the transparent lens 7 can be achieved, which can adapt to the needs of observing the three-dimensionality of cells under oblique light of a microscope and tracking cell migration paths from multiple angles, significantly expanding the observation dimensions.

[0049] Please see Figure 1 and Figure 2 A fixed frame plate 19 is provided below the circuit board 1. Limiting posts 20 are installed around the upper surface of the fixed frame plate 19, and the limiting posts 20 are inserted into the limiting holes 18. A fixed plate 21 is installed at the top center of the limiting posts 20. A screw 22 is rotatably connected to the top center of the upper surface of the fixed plate 21. An external connecting handle 23 is installed at the top of the screw 22. A sliding plate 24 is sleeved on the upper surface of the screw 22. An upper pressure plate 25 and a lower pressure plate 26 are rotatably connected to the outside of the fixed plate 21 on the sliding plate 24. The corresponding parts of the upper pressure plate 25 and the lower pressure plate 26 are rotatably connected.

[0050] Place the fixing frame plate 19 below the circuit board 1, align the limiting post 20 with the limiting hole 18, and then insert the limiting post 20 into the limiting hole 18. By connecting the limiting post 20 with the limiting hole 18, the initial positioning of the fixing frame plate 19 and the circuit board 1 is completed, so as to avoid relative displacement between the two in subsequent operations.

[0051] Operating the external connecting handle 23 drives the screw 22 to rotate around the center of the upper surface of the fixed plate 21, wherein the fixed plate 21 is installed at the top center of the limiting post 20, providing a stable rotation support base for the screw 22;

[0052] As the screw 22 rotates, the slide plate 24, which is sleeved on the upper part of the surface of the screw 22, moves along the axial direction of the screw 22. Since the upper pressure plate 25 is rotatably connected to the outside of the slide plate 24 and the lower pressure plate 26 is rotatably connected to the outside of the fixing plate 21, and the corresponding parts of the upper pressure plate 25 and the lower pressure plate 26 are rotatably connected, the movement of the slide plate 24 will synchronously drive the upper pressure plate 25 and the lower pressure plate 26 to rotate around their own rotational connection points with the slide plate 24 and the fixing plate 21, respectively. Continue to rotate the outer connecting handle 23 until the upper pressure plate 25 and the lower pressure plate 26 are together in contact with the surface of the circuit board 1, forming a stable clamp, and completing the fixing process of the circuit board 1.

[0053] The entire fixing process only requires rotating the outer connecting handle 23 to drive the screw 22, slide plate 24, upper pressure plate 25, and lower pressure plate 26 in linkage. No complicated tools are needed, and the operation steps are simple and easy to understand, reducing the difficulty of operation for operators and improving the efficiency of fixing operation. The upper pressure plate 25 and slide plate 24, the lower pressure plate 26 and fixing plate 21, and the upper pressure plate 25 and lower pressure plate 26 are all connected by rotation, so that the clamping angle of the upper pressure plate 25 and lower pressure plate 26 can be flexibly adjusted according to the actual thickness of the circuit board 1, which can adapt to the fixing requirements of circuit boards 1 of different specifications and expand the applicability of the device.

[0054] Please see Figure 3 The outer center of the slide plate 24 and the fixed plate 21 is provided with a groove, and the inner walls of the groove of the slide plate 24 and the fixed plate 21 are rotatably connected to the two sides of the groove. This allows the rotating shaft to rotate freely around its own axis relative to the inner wall of the groove. By providing a groove at the outer center of the slide plate 24 and the fixed plate 21, a precise installation and positioning space can be provided for the rotating shaft, avoiding positional displacement of the rotating shaft during assembly and ensuring the accuracy of subsequent component connections.

[0055] The ends of the rotating shafts inside the grooves of the sliding plate 24 and the fixed plate 21 are connected to the corresponding sides of the upper pressure plate 25 and the lower pressure plate 26. Connecting the ends of the rotating shafts inside the grooves of the fixed plate 21 to the corresponding sides of the lower pressure plate 26 ensures a firm connection between the ends of the rotating shafts and the lower pressure plate 26, completing the associated assembly of the rotating shafts with the upper pressure plate 25 and the lower pressure plate 26. This connection method directly establishes the transmission relationship between the rotating shafts and the upper pressure plate 25 and the lower pressure plate 26, allowing the rotation of the rotating shafts to be directly transmitted to the upper pressure plate 25 and the lower pressure plate 26, achieving synchronous motion linkage among the three and avoiding delays or failures in the motion transmission process. The design of connecting the ends of the rotating shafts to the corresponding sides of the upper pressure plate 25 and the lower pressure plate 26 allows the upper pressure plate 25 and the lower pressure plate 26 to bear the load evenly when under stress, preventing deformation or damage to the upper pressure plate 25 or the lower pressure plate 26 due to excessive local stress, thus extending the service life of the components.

[0056] A rotating rod is added between the corresponding positions of the upper pressure plate 25 and the lower pressure plate 26. The upper surface of the rotating rod is connected to the lower surface of the upper pressure plate 25, and the two ends of the rotating rod are rotatably connected to the upper sides of the lower pressure plate 26. The rotating rod is placed in the reserved installation space, so that the upper surface of the rotating rod is in contact with the lower surface of the upper pressure plate 25. Then, a suitable connection method is used to fix the upper surface of the rotating rod to the lower surface of the upper pressure plate 25, ensuring no relative displacement after connection. The two ends of the rotating rod are then assembled to the upper sides of the lower pressure plate 26 using a rotating connection structure, allowing the rotating rod to rotate freely around its own axis relative to the upper sides of the lower pressure plate 26, completing the overall assembly of the rotating rod with the upper pressure plate 25 and the lower pressure plate 26. The addition of the rotating rod further strengthens the connection between the upper pressure plate 25 and the lower pressure plate 26. The linkage stability prevents misalignment or separation of the upper pressure plate 25 and the lower pressure plate 26 during movement, ensuring the consistency of their movement directions. The structure of the rotating rod surface being fixedly connected to the lower surface of the upper pressure plate 25 and rotatably connected to the upper sides of the lower pressure plate 26 at both ends can not only effectively transmit the movement of the upper pressure plate 25 to the rotating rod, but also flexibly adjust the relative angle between the upper pressure plate 25 and the lower pressure plate 26 through the rotation of the rotating rod, improving the overall structure's movement flexibility, adapting to the movement requirements under different working conditions, and reducing direct friction loss between the upper pressure plate 25 and the lower pressure plate 26.

[0057] This solution involves placing a fixed frame plate 19 below the circuit board 1, aligning the limiting posts 20 installed around the upper surface of the fixed frame plate 19 with the limiting holes 18 opened around the upper surface of the circuit board 1, inserting the limiting posts 20 into the limiting holes 18, and operating the external connecting handle 23 installed at the top of the screw 22, which is rotatably connected to the center of the upper surface of the fixed plate 21 installed at the top center of the limiting post 20, to drive the screw 22 to rotate, causing the slide plate 24 sleeved on the upper part of the surface of the screw 22 to move along the axial direction of the screw 22. The upper pressure plate 25 and the lower pressure plate 26 rotate around their corresponding axes as the slide plate 24 moves, and the upper pressure plate 25 and the lower pressure plate 26 are rotatably connected by a rotating rod at their corresponding positions. The external connecting handle 23 is continuously rotated until the upper pressure plate 25 and the lower pressure plate 26 are in contact with the surface of the circuit board 1.

[0058] The culture plate is placed below the circuit board 1, and the bottom end of the gold plate extends into the culture well of the culture plate, so that the gold plate comes into contact with the cells in the culture well. At the same time, the material of the gold plate meets the requirements for cell electrical stimulation. Push the slider 12, and the ball 17 slides and adheres to the surface of the circuit board 1 until the slider 12 moves to the target position. Rotate the screw 16 threaded on both sides of the slider 12, so that the clamping blocks 15 set on both sides of the inner wall of the slider 12 are tightly attached to the surface of the guide rail 10, thereby fixing the slider 12.

[0059] Place the electrode 14 into the inner cavity of the gold-plated positioning hole 13 opened in the center of the upper surface of the slider 12, adjust the position of the electrode 14 to ensure that the electrode 14 is in close contact with the conductive block 11 installed in the center of the upper surface of the guide rail 10, and that the conductive block 11 is in contact with the gold-plated plate added to the inner wall of the pad hole 9 opened in the upper surface of the pad 8.

[0060] Connect the waterproof interface on the circuit board 1 to the external cell electrostimulator, start the external cell electrostimulator, and the electrode 14 will apply the stimulation signal to the target cell through the conductive block 11 and the gold plate. During this period, the transparent lens 7 can be driven by external force to drive the side connecting column 5 to rotate in the inner ring groove 3, adjust the angle of the transparent lens 7, and observe the cell state through the transparent lens 7.

[0061] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0062] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A petri dish stimulation electrode plate, characterized in that, include: The circuit board (1) includes a pad (8) and a waterproof interface disposed on the upper surface of the circuit board (1). The upper surface of the pad (8) is provided with a pad hole (9), and a gold plate is provided on the inner wall of the pad hole (9). The gold plate extends downward through the upper surface of the circuit board (1). A transparent lens (7) is provided on the upper surface of the circuit board (1). The transparent lens (7) is disposed between adjacent pads (8), and limit holes (18) are provided around the upper surface of the circuit board (1). The guide rail (10) is set on both sides of the pad (8), and the guide rail (10) is connected to the upper surface of the circuit board (1). A conductive block (11) is installed at the center of the upper surface of the guide rail (10), and the conductive block (11) is attached to the gold plate. The slider (12) is slidably connected to the center of the upper surface of the guide rail (10), and a gold-plated positioning hole (13) is opened at the center of the upper surface of the slider (12), and an electrode (14) is added in the inner cavity of the gold-plated positioning hole (13), and the electrode (14) is in contact with the corresponding part of the conductive block (11). Clamping blocks (15) are disposed on both sides of the inner wall of the slider (12), and screws (16) are threadedly connected to both sides of the slider (12). The inner end of the screws (16) is connected to the front of the corresponding clamping block (15), and the inner side of the clamping block (15) is tightly fitted to the surface of the guide rail (10). Balls (17) are rotatably connected to both sides of the lower surface of the slider (12), and the surface of the balls (17) slides against the surface of the circuit board (1).

2. The culture dish stimulation electrode plate according to claim 1, characterized in that: The upper surface of the circuit board (1) is provided with an annular groove (2), and the position of the annular groove (2) corresponds to the position of the transparent lens (7), and an inner annular groove (3) is provided at the center of the inner wall of the annular groove (2).

3. The culture dish stimulation electrode plate according to claim 2, characterized in that: An annular frame (4) is added to the outer surface of the transparent lens (7), and a sealing ring (6) is installed on the upper and lower parts of the outer side of the annular frame (4), and the surface of the sealing ring (6) is tightly fitted to the inner wall of the annular groove (2).

4. The culture dish stimulation electrode plate according to claim 3, characterized in that: The transparent lens (7) is rotatably connected to the center of both sides by side connecting posts (5), and the outer surface of the side connecting posts (5) is rotatably fitted with the inner wall of the inner ring groove (3).

5. A cell electrical stimulation device, characterized in that: The device includes a culture dish stimulation electrode plate as described in any one of claims 1-4. The cell electrostimulation device includes a fixed frame plate (19), which is located below the circuit board (1). Limiting posts (20) are installed around the upper surface of the fixed frame plate (19), and the limiting posts (20) are inserted into the limiting holes (18). A fixed plate (21) is installed at the top center of the limiting posts (20). A screw (22) is rotatably connected to the top center of the upper surface of the fixed plate (21). An external connecting handle (23) is installed at the top of the screw (22). A sliding plate (24) is sleeved on the upper surface of the screw (22). An upper pressure plate (25) and a lower pressure plate (26) are rotatably connected to the sliding plate (24) on the outside of the fixed plate (21), and the upper pressure plate (25) and the lower pressure plate (26) are rotatably connected at their corresponding positions.

6. A cell electrical stimulation device according to claim 5, characterized in that: The outer center of the slide plate (24) and the fixing plate (21) is provided with a groove, and the inner walls of the groove of the slide plate (24) and the fixing plate (21) are rotatably connected with a rotating shaft.

7. A cell electrical stimulation device according to claim 6, characterized in that: The ends of the rotating shafts inside the grooves on the sliding plate (24) and the fixing plate (21) are connected to the corresponding sides of the upper pressure plate (25) and the lower pressure plate (26).

8. A cell electrical stimulation device according to claim 5, characterized in that: A rotating rod is provided between the corresponding positions of the upper pressure plate (25) and the lower pressure plate (26), and the upper surface of the rotating rod is connected to the lower surface of the upper pressure plate (25), and the two ends of the rotating rod are rotatably connected to the upper sides of the lower pressure plate (26).

Images