An oncology cell culture dish structure
Patent Information
- Application Number
- CN202522122134.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-09
- Publication Date
- 2026-10-09
- Estimated Expiration
- 2035-10-09
AI Technical Summary
一方面，其密封性能有限，外部杂质容易进入皿体影响细胞培养环境，同时皿体内的培养液或细胞等物质也存在泄漏风险
[0010]与现有技术相比，本实用新型的有益效果是：该肿瘤学细胞培养皿结构，设置有小开孔，便于补充样品，降低样品补充中对培养环境的破坏，封堵件中的封堵盖板和密封片有效防止外部杂质进入和内部物质泄漏。观察部件的多个不同放大倍数的放大镜片，方便操作人员直接观察细胞不同尺度状态，节省时间并减少对额外设备的依赖。压力调节组件能够通过旋转柱、拧手、高度调节杆、刮片和螺杆的配合作用，改变培养区域内对样品涂覆或者其他操作过程中的压力。范围调节组件借助气囊、单向阀管路、充气柱、弹簧及滑动片，可根据样本培养区域或者涂覆区域灵活调整刮片长度，能够形成不同范围的细胞培养区域或者培养面积，为肿瘤学研究提供更可靠的实验条件。
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Figure CN224832733U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of culture dish technology, specifically to a structure of an oncology cell culture dish. Background Technology
[0002] In the field of oncology research, cell culture is an extremely important experimental method. Traditional cell culture dishes present several problems in practical use. Firstly, their sealing performance is limited, allowing external impurities to easily enter and affect the cell culture environment. Simultaneously, there is a risk of leakage of the culture medium or cells within the dish. Secondly, observing cell state during cell culture is not convenient or comprehensive enough, often requiring additional complex equipment to obtain cell morphology information at different magnifications, increasing the complexity and cost of experimental operations. Furthermore, in cell culture experiments, it is difficult to precisely control the pressure environment within the culture area and flexibly adjust the operating area according to the cell distribution, failing to adequately meet the demands of oncology cell culture research for precise operation and a stable environment. Therefore, there is an urgent need for an improved cell culture dish structure to overcome these shortcomings. Utility Model Content
[0003] The purpose of this invention is to provide a structure for an oncology cell culture dish to solve the problems mentioned in the background art.
[0004] To achieve the above objectives, this utility model provides the following technical solution: a tumor cell culture dish structure, comprising: Dish body; A top cover is disposed on the dish body. The top cover has a detachable sealing component and multiple observation components. The sealing component is used to close or open the opening of the top cover, and the observation components are used to observe the contents of the dish body. The center seat is fixedly placed at the middle of the bottom end of the inner cavity of the upper cover and is located inside the dish body; A pressure regulating assembly is connected to the central seat, and the pressure regulating assembly includes a scraper that can slide up and down; A range adjustment component is disposed within the central seat and is connected to the scraper blade to change the length of the scraper blade.
[0005] In one feasible embodiment, the sealing component includes: a sealing cover plate that can be detachably fastened into an opening in the upper cover; and a sealing sheet fixedly disposed on the outer wall of the sealing cover plate on the side away from the inner cavity of the vessel, the sealing sheet being attached to the outer end face of the upper cover.
[0006] In one feasible implementation, the observation component consists of multiple magnifying lenses with different magnifications, which are evenly distributed within the upper cover.
[0007] In one feasible embodiment, the pressure regulating assembly includes: a rotating column rotatably disposed within the central seat; a handle fixedly mounted on the outer wall of the top of the rotating column and protruding from the upper cover; a height adjusting rod movable along the height direction of the vessel, one end of the height adjusting rod being engaged within the central seat and the other end extending away from the central seat, the bottom end of the height adjusting rod being provided with a scraper; and a screw fixedly connected to the rotating column and coaxially disposed with the rotating column, the screw penetrating and screwed into the end of the height adjusting rod.
[0008] In one feasible implementation, the range adjustment assembly includes: an airbag fixedly disposed on the outside of the upper cover, and a one-way valve disposed at the airbag; an inflation column disposed in the inner cavity of the height adjustment rod, the length of the inflation column being adjustable; one end of a pipeline connected to the output end of the airbag, and the other end connected to the air inlet of the inflation column; and a spring abutting against the end of the inflation column away from the center seat.
[0009] In one feasible implementation, a plurality of sliding plates are fixedly disposed on the outer wall of the periphery of the inflatable column and are slidably disposed in the inner cavity of the height adjustment rod.
[0010] Compared with existing technologies, the beneficial effects of this invention are as follows: The oncology cell culture dish structure features small openings for easy sample replenishment, reducing disruption to the culture environment during sample replenishment. The sealing cover and sealing sheet in the sealing component effectively prevent external impurities from entering and internal substances from leaking. Multiple magnifying lenses with different magnifications in the observation component allow operators to directly observe cells at different scales, saving time and reducing reliance on additional equipment. The pressure adjustment component, through the combined action of a rotating column, a lever, a height adjustment rod, a scraper, and a screw, can change the pressure within the culture area during sample coating or other operations. The range adjustment component, utilizing an air bladder, a one-way valve pipeline, an inflation column, a spring, and a sliding plate, can flexibly adjust the scraper length according to the sample culture area or coating area, creating cell culture areas or cultures of varying sizes, providing more reliable experimental conditions for oncology research. Attached Figure Description
[0011] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a bottom-view explosion structure diagram of the present invention; Figure 3 This is a schematic diagram of the sealing cover plate in this utility model; Figure 4 This is a schematic diagram of the scraper blade in this utility model; Figure 5 This is a schematic diagram showing the position of the screw in this utility model; Figure 6 This is a schematic diagram of the sliding piece in this utility model.
[0012] In the diagram: 10. Dish body; 20. Top cover; 30. Sealing component; 40. Magnifying lens; 50. Center seat; 60. Pressure regulating component; 70. Range regulating component; 31. Sealing cover plate; 32. Sealing plate; 61. Rotating column; 62. Handle; 63. Height adjusting rod; 64. Scraper; 65. Screw; 71. Airbag; 72. One-way valve; 73. Pipeline; 74. Inflation column; 75. Sliding plate; 76. Spring. Detailed Implementation
[0013] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0014] Please see Figures 1 to 6 The present invention provides a technical solution: a tumor cell culture dish structure, comprising: a dish body 10, a top cover 20, a central seat 50, a pressure regulating component 60, and a range regulating component 70; The top cover 20 is disposed on the dish body 10. The top cover 20 has a detachable sealing member 30 and multiple observation components. The sealing member 30 is used to close or open the opening of the top cover 20, and the observation components are used to observe the inside of the dish body 10. The center seat 50 is fixedly disposed at the middle of the bottom end of the inner cavity of the top cover 20 and is located inside the dish body 10. The pressure regulating component 60 is connected to the center seat 50. The pressure regulating component 60 includes a scraper 64 that can slide up and down. The range regulating component 70 is disposed inside the center seat 50. The range regulating component 70 is connected to the scraper 64 and is used to change the length of the scraper 64.
[0015] In oncology cell culture, the dish 10 is used to contain the culture medium and cells. The detachable sealing component 30 of the top cover 20 controls the opening and closing of the opening, facilitating operations such as adding culture medium or cells. Multiple observation components allow for easy monitoring of the cell culture status within the dish 10. The central seat 50 is fixed in the middle of the bottom of the inner cavity of the top cover 20 and is located within the dish 10, serving to support and connect other components. The scraper 64 of the pressure regulating component 60 can slide up and down, and its working principle is to adjust the action of the scraper 64 on the culture dish by changing the height position of the scraper 64. Pressure, such as moving the scraper 64 towards the bottom wall of the dish 10, can increase the coating pressure; for example, a higher pressure results in a thinner coating film. The range adjustment component 70 is located within the central seat 50 and connected to the scraper 64. Through its function, the length of the scraper 64 can be changed, thereby enabling coating of cell culture areas of different sizes. For example, the scraper 64 can be lengthened when a large area of cells needs to be processed, and shortened when performing fine operations in a small area. Overall, this structure can precisely control the cell culture environment, reduce interference from external factors, and improve the stability and operability of cell culture.
[0016] In some examples, the sealing element 30 further includes a sealing cover 31 and a sealing sheet 32. The sealing cover 31 is detachably fastened into the opening of the upper cover 20. The sealing sheet 32 is fixedly disposed on the outer wall of the sealing cover 31 on the side away from the inner cavity of the vessel body 10, and the sealing sheet 32 is attached to the outer end face of the upper cover 20.
[0017] In this example, the sealing cover 31 can physically seal the opening of the upper cover 20. When it is necessary to add cells or culture medium into the dish 10, it can be removed from the opening and then fastened back after the operation is completed, ensuring that the internal environment of the dish 10 is relatively closed during the culture process. The sealing sheet 32 is fixed on the outer wall of the sealing cover 31 away from the inner cavity of the dish 10. When the sealing cover 31 is fastened in the opening of the upper cover 20, the sealing sheet 32 fits tightly against the outer end face of the upper cover 20, further enhancing the sealing effect, effectively preventing external impurities from entering the dish 10 and preventing leakage of substances inside the dish 10, reducing the interference of external factors on tumor cell culture, providing a stable and reliable environment for cell culture, and improving the accuracy and success rate of cell culture experiments.
[0018] In some examples, the observation component is further divided into multiple magnifying lenses 40 with different magnifications, which are evenly distributed within the top cover 20.
[0019] In this example, multiple magnifying lenses 40 with different magnifications are evenly distributed within the top cover 20 as observation components. During tumor cell culture, the operator can select the appropriate magnification lens according to the actual observation needs. When it is necessary to observe the general morphological distribution of cells, the low-magnification lens 40 can be used to quickly obtain the overall state of the cells. When it is necessary to observe the fine structural features of cells, the operator can switch to the high-magnification lens 40. Therefore, this culture dish does not require additional observation equipment and can directly achieve multi-scale observation through the different magnifying lenses 40 within the top cover 20, conveniently and quickly obtaining information about cells during the culture process, thus providing convenience for cell culture research.
[0020] In some examples, the pressure regulating assembly 60 further includes: a rotating column 61, a handle 62, a height adjusting rod 63, a scraper 64, and a screw 65. The rotating column 61 is rotatably disposed within the central seat 50. The handle 62 is fixedly disposed on the outer wall of the top of the rotating column 61 and protrudes from the upper cover 20. The height adjusting rod 63 is movable along the height direction of the vessel body 10. One end of the height adjusting rod 63 is engaged within the central seat 50, and the other end extends away from the central seat 50. A scraper 64 is provided at the bottom end of the height adjusting rod 63. The screw 65 is fixedly connected to the rotating column 61 and is coaxially disposed with the rotating column 61. The screw 65 passes through and is screwed into the end of the height adjusting rod 63.
[0021] In this example, the rotating column 61, as the central rotating component of the pressure regulating assembly 60, can rotate flexibly within the central seat 50; the handle 62 is fixed to the outer wall of the top of the rotating column 61 and protrudes from the upper cover 20, facilitating the operator to apply force to rotate the rotating column 61; the height adjusting rod 63 can move along the height direction of the dish body 10, one end of which is engaged in the central seat 50 for guidance and positioning, and the other end extends towards the edge of the dish body 10 with a scraper 64 at the bottom; the screw 65 is fixedly connected to the rotating column 61 and coaxial with it, passing through and screwing the end of the height adjusting rod 63; when the handle 62 is rotated to drive the rotating column 61 to rotate, the screw 65 rotates synchronously. Since the screw 65 is screwed to the height adjusting rod 63, under the action of the thread, the height adjusting rod 63 will move up and down along the height direction of the dish body 10, thereby driving the scraper 64 to rise and fall; in tumor cell culture, by adjusting the height of the scraper 64, the sample coating pressure can be changed, thereby applying corresponding pressure to samples with different properties for coating.
[0022] In some examples, the range adjustment assembly 70 further includes: an airbag 71, a one-way valve 72, a conduit 73, an inflation column 74, and a spring 76. The airbag 71 is fixedly disposed on the outside of the upper cover 20, and a one-way valve 72 is also disposed at the airbag 71. The inflation column 74 is disposed in the inner cavity of the height adjustment rod 63, and the length of the inflation column 74 can be varied. One end of the conduit 73 is connected to the output end of the airbag 71, and the other end is connected to the air inlet of the inflation column 74. The spring 76 abuts against the end of the inflation column 74 away from the center seat 50.
[0023] In this example, it can be understood that the airbag 71 contains gas and is located on the outside of the upper cover 20 for easy operation by the operator; the one-way valve 72 controls the unidirectional flow of gas, ensuring that gas can only enter the airbag 71 through this valve; the inflation column 74 is located inside the height adjustment rod 63 and has a variable length, used to change the length of the scraper 64; the pipe 73 connects the output end of the airbag 71 to the air inlet of the inflation column 74, serving as a gas transmission channel; the spring 76 abuts against one end of the inflation column 74 to inflate the column. 4. It provides support and reset; when the length of the scraper 64 needs to be adjusted, press the air bladder 71, and gas enters the inflation column 74 through the tube 73. The inflation column 74 extends under the action of air pressure, which in turn extends the scraper 64, expanding the range of action; when the scraper 64 needs to be shortened, close the one-way valve 72, and the spring 76 pushes the gas in the inflation column 74 to be discharged, shortening the inflation column 74 and the scraper 64 accordingly; in oncology cell culture, the length of the scraper 64 can be flexibly adjusted according to the cell distribution range, allowing for precise operation of the cell culture area.
[0024] It should be noted that there are multiple scraper blades 64, which are fitted together and arranged in parallel. Each scraper blade 64 has a fixed connection point with the inflation column 74, such as a connecting block. The connecting block can fix the scraper blade 64 to maintain its shape after being compressed. As the side length of the inflation column 74 increases, each connecting block and scraper blade 64 moves synchronously and uniformly with the deformation of the inflation column 74, thus forming scraper blades 64 with different length variations.
[0025] In some examples, further, several sliding pieces 75 are fixedly mounted on the outer wall around the inflation column 74 and are slidably disposed in the inner cavity of the height adjustment rod 63.
[0026] Understandably, several sliding plates 75 are fixed to the outer wall of the inflation column 74 and can slide within the height adjustment rod 63. Their function is to provide stable support and guidance for the inflation column 74. When the length of the inflation column 74 changes due to the inflow and outflow of gas, the sliding plates 75 slide along the inner cavity of the height adjustment rod 63, restricting the direction of movement of the inflation column 74 and ensuring that the inflation column 74 can only deform uniformly in the radial direction of the center seat 50, thereby ensuring that the scraper 64 can be accurately adjusted in position. At the same time, the sliding of the sliding plates 75 reduces the frictional resistance between the inflation column 74 and the inner cavity of the height adjustment rod 63, making the extension and retraction process of the inflation column 74 smoother.
[0027] Its detailed connection method is a well-known technology in this field. The following mainly introduces the working principle and process, and the specific work is as follows.
[0028] During tumor cell culture, the top cover 20 is closed or opened via a detachable sealing component 30 (composed of a sealing cover plate 31 and a sealing sheet 32). The sealing sheet 32 enhances the sealing effect to prevent impurities from entering or the culture medium from leaking. The observation component (multiple magnifying lenses 40 with different magnifications) can be directly used to observe the cells at different scales. In the pressure regulating component 60, turning the handle 62 protruding from the top cover 20 causes the rotating column 61 to rotate, which in turn drives the coaxial screw 65 to rotate. The height adjusting rod 63, which is screwed to the screw 65, moves along the height direction of the dish body 10, and its bottom scraper 64 rises accordingly. The pressure applied to the sample is adjusted by lowering the pressure. Within the range adjustment assembly 70, pressing the air bladder 71 on the outside of the top cover 20 allows gas to enter the inflation column 74 within the height adjustment rod 63 via the one-way valve 72 and pipeline 73. The inflation column 74 elongates under pressure (several sliding plates 75 assist in its uniform deformation and reduce frictional resistance), causing the scraper 64 to elongate and expand its effective range. Releasing the one-way valve 72 pushes the gas out through the spring 76, causing the inflation column 74 to shorten. This allows for flexible adjustment of the scraper 64 length according to cell distribution, enabling precise manipulation of the cell culture area and providing excellent experimental conditions for oncology cell culture research.
[0029] In the description of this utility model, it should be understood that the terms "coaxial," "bottom," "one end," "top," "middle," "other end," "upper," "side," "top," "inner," "front," and "both ends," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. At the same time, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "fixed installation," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two elements or the interaction relationship between two elements. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0030] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A structure for an oncology cell culture dish, characterized in that, include: Dish body (10); The top cover (20) is disposed on the dish body (10). The top cover (20) has a detachable sealing member (30) and multiple observation components. The sealing member (30) is used to close or open the opening of the top cover (20), and the observation components are used to observe the inside of the dish body (10). The center seat (50) is fixedly placed at the middle of the bottom of the inner cavity of the upper cover (20) and is located inside the dish body (10); A pressure regulating assembly (60) is connected to the center seat (50), and the pressure regulating assembly (60) includes a scraper (64) that can slide up and down. A range adjustment component (70) is disposed within the center seat (50). The range adjustment component (70) is connected to the scraper (64) and is used to change the length of the scraper (64).
2. The oncology cell culture dish structure according to claim 1, characterized in that: The sealing element (30) includes: The sealing cover (31) is detachably fastened to the opening in the upper cover (20); A sealing sheet (32) is fixedly disposed on the outer wall of the sealing cover plate (31) on the side away from the inner cavity of the vessel body (10), and the sealing sheet (32) is attached to the outer end face of the upper cover (20).
3. The oncology cell culture dish structure according to claim 1, characterized in that: The observation component consists of multiple magnifying lenses (40) with different magnifications, which are evenly distributed within the upper cover (20).
4. The oncology cell culture dish structure according to claim 3, characterized in that: The pressure regulating assembly (60) includes: A rotating column (61) is rotatably disposed within the central seat (50); The handle (62) is fixedly placed on the top outer wall of the rotating column (61) and protrudes from the top cover (20). The height adjustment rod (63) is movable along the height direction of the dish body (10). One end of the height adjustment rod (63) is engaged in the center seat (50), and the other end extends away from the center seat (50). A scraper (64) is provided at the bottom end of the height adjustment rod (63). The screw (65) is fixedly connected to the rotating column (61) and is coaxially arranged with the rotating column (61). The screw (65) passes through and is screwed into the end of the height adjusting rod (63).
5. The oncology cell culture dish structure according to claim 4, characterized in that: The range adjustment component (70) includes: An airbag (71) is fixedly installed on the outside of the upper cover (20), and a one-way valve (72) is also provided at the airbag (71). An inflatable column (74) is disposed in the inner cavity of the height adjustment rod (63), and the length of the inflatable column (74) can be varied. The pipeline (73) is connected at one end to the output end of the airbag (71) and at the other end to the air inlet of the inflation column (74); The spring (76) abuts against the end of the inflatable column (74) away from the center seat (50).
6. The oncology cell culture dish structure according to claim 5, characterized in that: Several sliding pieces (75) are fixedly arranged on the outer wall of the periphery of the inflatable column (74) and can be slidably arranged in the inner cavity of the height adjustment rod (63).