A device for pre-treatment for total bird isotope analysis

Abstract

The utility model discloses a kind of for whole bird isotopic analysis with pretreatment device, belong to sample pretreatment technical field.The device includes deep cooling pretreatment module, multistage grinding system, vacuum homogenizing device and sample collection and drying module sequentially connected along material flow direction.Deep cooling pretreatment module is frozen by liquid nitrogen spraying to whole bird and is sent to multistage grinding system by screw propeller;Multistage grinding system is first coarsely crushed by cutting gear, then gradually fine grinding is carried out by three-stage staggered tooth grinding disc;Vacuum homogenizing device carries out powerful homogenization to the mixture after grinding under the negative pressure environment using double-shaft agitator, and finally the collection and vacuum drying of sample are completed by sample collection and drying module.The utility model is through the synergistic effect of deep cooling, multistage grinding and vacuum homogenization, first realizes the efficient, uniform mixing of feather and flesh and other tissues in whole bird sample, effectively prevents sample denaturation and oxidation, whole process automation, closed operation, significantly improves the representativeness, accuracy and processing efficiency of whole bird isotopic analysis sample.

Description

Technical Field

[0001] This utility model relates to the technical field of sample pretreatment technology, and in particular to a pretreatment device for whole bird isotope analysis. Background Technology

[0002] Whole-bird isotope analysis is a key technique for studying ecological issues such as bird migration, diet, trophic level, and the transport of environmental pollutants. The accuracy of the analytical results is highly dependent on the representativeness of the samples prepared in the pretreatment stage. The ideal pretreatment sample should be a homogeneous body that has been thoroughly and uniformly mixed with bird tissues such as feathers, muscles, bones, and internal organs to ensure that the trace sample extracted from it can truly reflect the isotope ratios of the whole bird.

[0003] However, existing technologies lack dedicated pretreatment devices capable of efficiently and completely processing whole bird samples. Researchers typically rely on manual labor or traditional equipment for stepwise processing, which has the following significant drawbacks:

[0004] The challenge of feather processing: Bird feathers are primarily composed of tough keratin, whose physicochemical properties differ significantly from those of soft tissue. Traditional grinding equipment (such as tissue grinders and ball mills) struggles to simultaneously and uniformly pulverize feathers and body tissue. Common practices involve manually cutting the feathers or grinding them separately from the body tissue before physical mixing. This method is not only inefficient and labor-intensive but also highly susceptible to human error, failing to guarantee uniform mixing of feathers and body tissue at the microscopic scale, thus leading to deviations in isotope measurements.

[0005] Sample cross-contamination and thermal denaturation risks: When using general-purpose grinding equipment to process different samples sequentially, incomplete cleaning can lead to serious cross-contamination. Furthermore, if the frictional heat generated during grinding is not effectively controlled, it may cause protein denaturation and isotope fractionation in the samples, affecting the accuracy of the analytical results.

[0006] The process is cumbersome and lacks automation: complete sample pretreatment requires multiple independent steps such as freezing, cutting, grinding, homogenizing, and drying. Transferring samples between different devices is not only time-consuming but also increases the risk of sample loss and contamination. Current technology lacks an integrated automated solution that seamlessly connects these processes.

[0007] Insufficient destruction of tissue integrity: Conventional cutting or grinding methods are difficult to completely break down hard tissues such as bones and keratin in one go for whole bird samples, which may lead to the loss of certain components in the sample and affect the overall representativeness.

[0008] To address the aforementioned problems, particularly to achieve efficient and uniform mixing of feathers and meat, there is an urgent need for a specialized, automated whole-bird isotope analysis sample pretreatment device. This device should be capable of deep freezing and embrittlement of whole-bird samples, multi-stage progressive grinding, vacuum homogenization while preventing denaturation, and finally, sample collection and drying, forming a continuous, closed, and controllable processing flow that fundamentally guarantees the representativeness and accuracy of the final analytical sample. Utility Model Content

[0009] The purpose of this invention is to provide a pretreatment device for whole-bird isotope analysis to overcome the shortcomings of the prior art.

[0010] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0011] A pretreatment device for whole-bird isotope analysis includes a cryogenic pretreatment module, a multi-stage grinding system, a vacuum homogenizer, and a sample collection and drying module. The cryogenic pretreatment module includes a blast freezer with an inlet and an outlet, and a propeller inside the blast freezer. The blast freezer is used to cryogenically embrittle whole-bird samples, and the propeller is used to transport the cryogenically treated samples. The multi-stage grinding system includes a coarse grinding structure and a fine grinding structure connected in sequence. The outlet of the blast freezer is connected to the inlet of the coarse grinding structure, which coarsely grinds the sample. The fine grinding structure is used to finely grind the coarsely ground sample. The outlet of the fine grinding structure is connected to the inlet of the vacuum homogenizer. The vacuum homogenizer includes a tank, a stirrer inside the tank, and a vacuum pump connected to the tank. The stirrer is used to stir the sample inside the tank, and the vacuum pump is used to evacuate the tank. The sample collection and drying module is located below the outlet of the vacuum homogenizer and is used to collect the finely ground sample and dry it.

[0012] Further improvements to optimize the technical solution include:

[0013] The quick-freezing chamber has an inlet at the front and an outlet at the rear. The propeller consists of a propulsion shaft and helical blades mounted on the propulsion shaft. The front end of the propulsion shaft extends outside the quick-freezing chamber and is connected to a propulsion motor. The propulsion motor is fixedly installed outside the quick-freezing chamber and drives the propeller to rotate, thereby causing the helical blades to propel the whole bird sample backward.

[0014] The quick-freezing chamber is equipped with liquid nitrogen spray pipes and spray heads. The liquid nitrogen spray pipes are connected to the spray heads, and there are several spray heads installed inside the quick-freezing chamber. The liquid nitrogen spray pipes can spray liquid nitrogen into the quick-freezing chamber through the spray heads, thereby performing cryogenic embrittlement treatment on the whole bird samples.

[0015] The coarse crushing structure includes a coarse crushing chamber, a first cutting gear, and a second cutting gear. The coarse crushing chamber has an inlet at its upper end, which is connected to the outlet of the quick-freezing chamber. The coarse crushing chamber has an outlet at its lower end. The first cutting gear and the second cutting gear are rotatably mounted in the coarse crushing chamber. Cutting blades are provided on the circumference of the first cutting gear and the second cutting gear, and the first cutting gear and the second cutting gear mesh with each other. The first cutting gear and / or the second cutting gear are connected to a cutting motor, which can drive the first cutting gear and the second cutting gear to rotate, thereby cutting the sample entering the coarse crushing chamber.

[0016] The fine grinding structure includes a fine grinding chamber, a three-stage grinding disc unit, and a grinding disc motor. The fine grinding chamber has an inlet at the top, which is connected to the outlet of the coarse grinding chamber. The fine grinding chamber has an outlet at the bottom. The grinding disc motor is fixed outside the fine grinding chamber. The three-stage grinding disc unit is installed inside the fine grinding chamber. The grinding disc motor is connected to the three-stage grinding disc unit through a drive shaft. The grinding disc motor drives the three-stage grinding disc unit to grind the sample entering the fine grinding chamber.

[0017] The three-stage grinding disc unit comprises three grinding disc units arranged sequentially, one above the other. Each grinding disc unit includes a collection tube, an upper grinding disc, a lower grinding disc, a connecting plate, a discharge port, and a collection tray. The collection tube passes through the non-axial position of the upper grinding disc and is used to deliver the sample between the upper and lower grinding discs. The upper and lower grinding discs are stacked and fitted together, and their mating surfaces are provided with grinding teeth. The drive shaft passes through the axis of the lower grinding disc and is fixedly connected to the axis of the upper grinding disc. The drive shaft and the lower grinding disc are clearance-fitted. The bottom of the lower grinding disc is fixedly connected to the wall of the fine grinding chamber through the connecting plate. The connecting plate has a vertically penetrating discharge port that allows the sample to leak to the next level grinding disc unit. The collection tray is fixedly connected to the drive shaft, and the upper end of the collection tube communicates with the collection tray. The collection tray is used to collect the sample entering the fine grinding chamber or the sample leaking from the previous level grinding disc unit.

[0018] Both the coarse crushing chamber and the fine crushing chamber are equipped with coolant circulation pipes on their outer walls, which are connected to an external coolant circulation unit.

[0019] The agitator is a twin-shaft agitator.

[0020] Solenoid valves are installed at the connection points between the cryogenic pretreatment module and the multi-stage grinding system, the connection points between the multi-stage grinding system and the vacuum homogenizer, and the outlet of the vacuum homogenizer. The solenoid valves are used to control the on / off state of the connection points.

[0021] The sample collection and drying module includes a collection tray and a vacuum drying chamber. The collection tray is used to receive the sample discharged from the outlet of the vacuum homogenizer, and the vacuum drying chamber is used to dry the sample in the collection tray.

[0022] The technical advantages of this utility model are as follows:

[0023] 1. This invention, through the synergistic effect of "cryogenic embrittlement - multi-stage grinding - vacuum homogenization," achieves for the first time the simultaneous and efficient homogenization of tough feathers and soft tissues in whole bird samples. Cryogenic treatment embrittles the keratin in the feathers, fundamentally altering their physical properties, allowing them to be effectively broken down along with the meat during subsequent mechanical grinding. The multi-stage grinding system (coarse crushing + fine grinding) achieves progressive refinement of the material. Finally, vacuum homogenization ensures a stable and uniform mixture of feather fragments and meat paste at the microscopic scale. This completely avoids the errors caused by traditional manual separation processes, ensuring that the final analytical sample has perfect representativeness.

[0024] 2. The integrated coolant circulation system of the device can continuously and effectively control the temperature throughout the grinding and homogenization process, completely eliminating protein denaturation caused by frictional heat and potential isotope fractionation. Simultaneously, the vacuum homogenizer operates in an oxygen-free environment, preventing sample oxidation and perfectly preserving the original chemical and isotopic information of the sample, providing a fundamental guarantee for obtaining accurate and reliable isotopic data.

[0025] 3. This invention achieves full-process automation and integration, significantly improving efficiency and consistency: From whole bird sample introduction to homogenized and dried sample output, this invention achieves a fully closed-loop, automated operation. It eliminates sample transfer between different devices and manual intervention, greatly improving processing efficiency, reducing labor intensity, and minimizing the risk of human error and sample cross-contamination. The modular processing also ensures a high degree of consistency and comparability of sample processing results across different batches and by different operators.

[0026] 4. This device can not only process soft tissues such as muscles, but also effectively break down feathers, beaks, claws and even small bones after cryogenic embrittlement, achieving near-complete utilization of whole bird samples. This avoids the problem of sample representativeness loss caused by the difficulty in processing certain components, making the isotope analysis results more reflective of the overall condition of the organism. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of this utility model;

[0028] Figure 2 This is a schematic diagram of the cryogenic pretreatment module;

[0029] Figure 3 This is a schematic diagram of a multi-stage grinding system;

[0030] Figure 4 This is a schematic diagram of a vacuum homogenizing device.

[0031] The attached diagram is labeled as follows: Cryogenic pretreatment module 1, quick-freezing chamber 11, screw propeller 12, quick-freezing chamber inlet 13, quick-freezing chamber outlet 14, propulsion motor 15, liquid nitrogen spray pipeline 16, spray head 17, multi-stage grinding system 2, coarse crushing structure 21, coarse crushing chamber 21a, first cutting gear 21b, second cutting gear 21c, fine grinding structure 22, fine crushing chamber 22a, grinding disc motor 22b, drive shaft 22c, three-stage grinding disc unit 23, collection pipe 23a, upper grinding disc 23b, lower grinding disc 23c, connecting plate 23d, material outlet 23e, collection tray 23f, vacuum homogenizer 3, tank 31, stirrer 32, vacuum pump 33, sample collection and drying module 4, collection tray 41, vacuum drying box 42, coolant circulation pipe 5. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of this application clearer, the application is described and illustrated below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application. All other embodiments obtained by those skilled in the art based on the embodiments provided in this application without inventive effort are within the scope of protection of this application.

[0033] Obviously, the accompanying drawings described below are merely some examples or embodiments of this application. Those skilled in the art can apply this application to other similar scenarios based on these drawings without any inventive effort. Furthermore, it is understood that although the efforts made in this development process may be complex and lengthy, for those skilled in the art related to the content disclosed in this application, any changes to design, manufacturing, or production based on the technical content disclosed in this application are merely conventional technical means and should not be construed as insufficient disclosure of the content of this application.

[0034] like Figure 1 As shown, this utility model provides a pretreatment device for whole-bird isotope analysis. The device is a linear processing line, mainly including a cryogenic pretreatment module 1, a multi-stage grinding system 2, a vacuum homogenizing device 3, and a sample collection and drying module 4.

[0035] like Figure 2As shown, the cryogenic pretreatment module 1 includes a horizontal cylindrical quick-freezing chamber 11. The quick-freezing chamber 11 has a quick-freezing chamber inlet 13 at its front end and a quick-freezing chamber outlet 14 at its rear end. Inside the quick-freezing chamber 11 is a propeller 12, which consists of a propulsion shaft and helical blades welded to the propulsion shaft. The front end of the propulsion shaft extends to the outside of the quick-freezing chamber 11 through a mechanical seal structure and is connected to a propulsion motor 15 fixed to the outer wall of the quick-freezing chamber 11. When the propulsion motor 15 operates, it drives the propeller 12 to rotate, thereby propelling the whole bird sample placed inside the quick-freezing chamber 11 backward at a uniform speed. To achieve rapid deep freezing, the quick-freezing chamber 11 is equipped with a liquid nitrogen spray pipeline 16, on which multiple spray heads 17 are connected in parallel. The liquid nitrogen spray pipe 16 is connected to an external liquid nitrogen storage tank, which can spray liquid nitrogen into the quick-freezing chamber 11, so that the temperature inside the chamber drops rapidly to below -80°C, thereby performing deep cryogenic embrittlement treatment on the whole bird sample, making its feathers and tissues brittle and easy to break in the future.

[0036] like Figure 3 As shown, the multi-stage grinding system 2 includes a coarse grinding structure 21 and a fine grinding structure 22 connected sequentially from top to bottom. The quick-freezing chamber outlet 14 is connected to the inlet of the coarse grinding structure 21 via a pipe.

[0037] The coarse crushing structure 21 mainly includes a coarse crushing chamber 21a, a first cutting gear 21b, and a second cutting gear 21c. The first cutting gear 21b and the second cutting gear 21c are rotatably supported within the coarse crushing chamber 21a via bearings. Both have sharp cutting blades on their circumferential surfaces, which mesh with each other. One of the cutting gears is connected to a cutting motor installed outside the coarse crushing chamber 21a. After the cryogenically embrittled whole bird sample is pushed from the quick-freezing chamber 11 into the coarse crushing chamber 21a, it is immediately cut and torn into centimeter-sized pieces by the high-speed, counter-rotating first cutting gear 21b and second cutting gear 21c.

[0038] The fine grinding structure 22 is located below the coarse crushing structure 21, and includes a fine grinding chamber 22a, a grinding disc motor 22b, and a three-stage grinding disc unit 23. The upper inlet of the fine grinding chamber 22a is directly connected to the lower outlet of the coarse crushing chamber 21a. The grinding disc motor 22b is fixed to the bottom of the fine grinding chamber 22a, and its output shaft is connected to a vertical drive shaft 22c that extends into the interior of the fine grinding chamber 22a.

[0039] The three-stage grinding disc unit 23 is composed of three identical grinding disc units connected in series from top to bottom. Each grinding disc unit includes a collection pipe 23a, an upper grinding disc 23b, a lower grinding disc 23c, a connecting plate 23d, a discharge port 23e, and a collection tray 23f. The upper grinding disc 23b, collection tray 23f, and collection pipe 23a are all fixedly connected to the drive shaft 22c and rotate together with it. The lower grinding disc 23c is fixedly connected to the wall of the fine grinding chamber 22a via the connecting plate 23d and remains stationary. Hard grinding teeth are machined on the mating surfaces of the upper grinding disc 23b and the lower grinding disc 23c. The connecting plate 23d has a through discharge port 23e.

[0040] The process is as follows: After coarse crushing, the sample falls into the collection tray 23f of the first-stage grinding unit and is thrown into the collection pipe 23a under centrifugal force. The collection pipe 23a guides the material to the grinding area between the upper grinding tray 23b and the lower grinding tray 23c. Due to the high-speed rotation of the upper grinding tray 23b and the stationary position of the lower grinding tray 23c, a strong shearing and grinding force is generated, which finely grinds the material. The ground material leaks through the discharge port 23e into the collection tray 23f of the next-stage grinding unit, and the above process is repeated to achieve progressive refinement of the material. After three stages of grinding, the sample becomes a fine mixture containing feather fragments and meat paste.

[0041] To control the heat generated during the grinding process, coolant circulation pipes 5 are coiled around the outer walls of both the coarse grinding chamber 21a and the fine grinding chamber 22a. These coolant circulation pipes 5 are connected to an external coolant circulation unit (not shown in the figure), and by continuously circulating a low-temperature coolant (such as an aqueous solution of ethylene glycol), the grinding process is ensured to always be carried out at a low temperature, effectively preventing protein denaturation in the sample.

[0042] like Figure 4 As shown, the vacuum homogenizing device 3 includes a sealed tank 31. The top inlet of the tank 31 is connected to the outlet of the fine grinding structure 22 via a solenoid valve to ensure that the vacuum inside the tank is not disrupted during feeding. A dual-shaft stirrer 32 is installed inside the tank 31; the two shafts can be driven independently to achieve a synergistic effect of strong shearing and overall tumbling. A vacuum pump 33 is connected to the top of the tank 31 via a pipe for evacuating the tank, preventing sample oxidation and removing air bubbles entrained during stirring. The finely ground mixture is further homogenized under vacuum to form an extremely stable and homogeneous slurry.

[0043] The sample collection and drying module 4 is located below the discharge port of the vacuum homogenizer 3. It mainly includes a movable collection tray 41 and a vacuum drying chamber 42. After homogenization, the discharge valve is opened, and the homogenized sample is discharged into the collection tray 41. Subsequently, the collection tray 41 is moved entirely into the vacuum drying chamber 42 and dried under 60°C and negative pressure conditions to finally obtain a dry, homogeneous sample that meets the requirements for isotope measurement.

[0044] Solenoid valves are installed along the material flow path of the entire device, including the connection between the cryogenic pretreatment module 1 and the multi-stage grinding system 2, the connection between the multi-stage grinding system 2 and the vacuum homogenizer 3, and the outlet of the vacuum homogenizer 3. These valves are used to precisely control the flow of materials in each stage and achieve automated process control.

[0045] The above are merely preferred embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in this utility model, based on the technical solution and inventive concept of this utility model, should be included within the scope of protection of this utility model.

Claims

1. A pretreatment apparatus for whole-bird isotope analysis, characterized in that, The system includes a cryogenic pretreatment module (1), a multi-stage grinding system (2), a vacuum homogenizer (3), and a sample collection and drying module (4). The cryogenic pretreatment module (1) includes a quick-freezing chamber (11) with an inlet and an outlet, and a screw propeller (12) installed inside the quick-freezing chamber (11). The quick-freezing chamber (11) is used for cryogenic embrittlement treatment of whole bird samples, and the screw propeller (12) is used to transport the cryogenically treated samples. The multi-stage grinding system (2) includes a coarse crushing structure (21) and a fine grinding structure (22) connected in sequence. The outlet of the quick-freezing chamber (11) is connected to the inlet of the coarse crushing structure (21). The coarse crushing structure (21) is used for... The sample is coarsely crushed, and the fine grinding structure (22) is used to finely grind the coarsely crushed sample. The outlet of the fine grinding structure (22) is connected to the inlet of the vacuum homogenizer (3). The vacuum homogenizer (3) includes a tank (31), a stirrer (32) installed in the tank (31), and a vacuum pump (33) connected to the tank (31). The stirrer (32) is used to stir the sample in the tank (31), and the vacuum pump (33) is used to evacuate the tank (31). The sample collection and drying module (4) is installed below the outlet of the vacuum homogenizer (3). The sample collection and drying module (4) is used to collect the finely ground sample and dry the sample.

2. The pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, The quick-freezing chamber (11) has a quick-freezing chamber inlet (13) at the front end and a quick-freezing chamber outlet (14) at the rear end. The spiral propeller (12) consists of a propulsion shaft and spiral blades mounted on the propulsion shaft. The front end of the propulsion shaft extends out of the quick-freezing chamber (11) and is connected to the propulsion motor (15). The propulsion motor (15) is fixedly installed outside the quick-freezing chamber (11). The propulsion motor (15) can drive the spiral propeller (12) to rotate, thereby causing the spiral blades to propel the whole bird sample backward.

3. The pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, The quick-freezing chamber (11) is equipped with a liquid nitrogen spray pipe (16) and a spray head (17). The liquid nitrogen spray pipe (16) is connected to the spray head (17). There are several spray heads (17) installed in the quick-freezing chamber (11). The liquid nitrogen spray pipe (16) can spray liquid nitrogen into the quick-freezing chamber (11) through the spray head (17) to perform deep cryogenic embrittlement treatment on the whole bird sample.

4. The pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, The coarse crushing structure (21) includes a coarse crushing chamber (21a), a first cutting gear (21b), and a second cutting gear (21c). The coarse crushing chamber (21a) has an inlet at its upper end, which is connected to the outlet of the quick-freezing chamber (11). The coarse crushing chamber (21a) has an outlet at its lower end. The first cutting gear (21b) and the second cutting gear (21c) are rotatably arranged in the coarse crushing chamber (21a). Cutting blades are provided on the circumference of the first cutting gear (21b) and the second cutting gear (21c), and the first cutting gear (21b) and the second cutting gear (21c) mesh with each other. The first cutting gear (21b) and / or the second cutting gear (21c) are connected to a cutting motor. The cutting motor can drive the first cutting gear (21b) and the second cutting gear (21c) to rotate, thereby cutting the sample entering the coarse crushing chamber (21a).

5. A pretreatment apparatus for whole-bird isotope analysis according to claim 4, characterized in that, The fine grinding structure (22) includes a fine grinding chamber (22a), a three-stage grinding disc unit (23), and a grinding disc motor (22b). The fine grinding chamber (22a) has an inlet at its upper end, which is connected to the outlet of the coarse grinding chamber (21a). The fine grinding chamber (22a) has an outlet at its lower end. The grinding disc motor (22b) is fixed outside the fine grinding chamber (22a). The three-stage grinding disc unit (23) is installed inside the fine grinding chamber (22a). The grinding disc motor (22b) is connected to the three-stage grinding disc unit (23) through a drive shaft (22c). The grinding disc motor (22b) drives the three-stage grinding disc unit (23) to grind the sample entering the fine grinding chamber (22a).

6. A pretreatment apparatus for whole-bird isotope analysis according to claim 5, characterized in that, The three-stage grinding disc unit (23) includes three grinding disc units arranged vertically. Each grinding disc unit includes a collection pipe (23a), an upper grinding disc (23b), a lower grinding disc (23c), a connecting plate (23d), a discharge port (23e), and a collection tray (23f). The collection pipe (23a) passes through the non-axial position of the upper grinding disc (23b) vertically. The collection pipe (23a) is used to deliver the sample between the upper grinding disc (23b) and the lower grinding disc (23c). The upper grinding disc (23b) and the lower grinding disc (23c) are stacked vertically and fitted together. The mating surfaces of the two discs are provided with grinding teeth. The drive shaft (22c) passes through the lower grinding disc (23c). The shaft is fixedly connected to the shaft of the upper grinding disc (23b), the drive shaft (22c) is clearance-fitted with the lower grinding disc (23c), the bottom of the lower grinding disc (23c) is fixedly connected to the wall of the fine grinding chamber (22a) through the connecting plate (23d), the connecting plate (23d) is provided with a through-hole (23e) for the material to leak to the next level grinding disc unit, the collection disc (23f) is fixedly connected to the drive shaft (22c), the upper end of the collection pipe (23a) is connected to the collection disc (23f), and the collection disc (23f) is used to collect the sample entering the fine grinding chamber (22a) or the sample leaking from the previous level grinding disc unit.

7. A pretreatment apparatus for whole-bird isotope analysis according to claim 6, characterized in that, The outer walls of both the coarse crushing chamber (21a) and the fine crushing chamber (22a) are provided with coolant circulation pipes (5), which are connected to an external coolant circulation unit.

8. A pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, The agitator (32) is a twin-shaft agitator.

9. A pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, Solenoid valves are provided at the connection points of the cryogenic pretreatment module (1) and the multi-stage grinding system (2), the connection points of the multi-stage grinding system (2) and the vacuum homogenizer (3), and the outlet of the vacuum homogenizer (3). The solenoid valves are used to control the on / off state of the connection points.

10. A pretreatment apparatus for whole-bird isotope analysis according to claim 1, characterized in that, The sample collection and drying module (4) includes a collection tray (41) and a vacuum drying box (42). The collection tray (41) is used to receive the sample released from the outlet of the vacuum homogenizer (3), and the vacuum drying box (42) is used to dry the sample in the collection tray (41).

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