Electron microscope specimen section staining device

Abstract

The utility model relates to the technical field of specimen section dyeing, especially electron microscope specimen section dyeing device, including base plate, the top fixedly connected with installation cover shell of base plate, the middle part of installation cover shell inner chamber rear side is connected with linear motor through bolt, the front side fixedly connected with drip tube of linear motor, the bottom fixedly connected with fixed crossbeam of installation cover shell rear side, both sides of the front and back of fixed crossbeam top all are fixedly connected with support frame, the top of support frame penetrates and is connected with draw -bar of sliding, the surface of draw -bar is set up with clamping spring, the bottom fixedly connected with clamping plate of draw -bar. Drive motor drives stirring rod rotation to carry out the stirring to the dye in liquid storage tank, and the dye is extracted through the flexible hose and is delivered to the drip tube in micro -pump, finally drops on the specimen section on the glass slide below, like this can avoid the dye to appear settlement, improves dyeing quality.

Description

Technical Field

[0001] This utility model relates to the field of specimen section staining technology, specifically to an electron microscope specimen section staining device. Background Technology

[0002] An electron microscope specimen staining instrument is a device specifically designed for staining specimen sections under an electron microscope. It can improve the efficiency of electron microscope specimen staining and reduce the workload of researchers. Its main function is to stain tissue samples and fix them on glass slides for observation and analysis under a microscope.

[0003] Currently, most methods involve staining samples spread on a grid with metal salt dyes, resulting in the entire grid being coated with a layer of heavy metal salts. Existing slide staining devices cannot agitate the dyes, leading to sedimentation due to prolonged standing. This uneven distribution of dye components affects staining quality. Furthermore, the devices do not provide sufficient fixation for the slides, and instrument movement can cause slide shifting, altering the dye spray position and resulting in inaccurate staining, thus hindering experimental progress. To address these technical issues, we have designed an electron microscope specimen slide staining device. Utility Model Content

[0004] The purpose of this invention is to provide an electron microscope specimen slide staining device that has the advantages of stirring the dye to prevent sedimentation, improving staining quality, fixing the slide more firmly, ensuring the accuracy of staining position, and improving the experimental research process. It solves the problems of dye sedimentation when left to stand for a long time, which affects staining quality, and insufficient fixation of the slide, which affects the experimental research process.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an electron microscope specimen section staining device, comprising a base plate, a mounting cover fixedly connected to the top of the base plate, a linear motor bolted to the middle of the rear side of the inner cavity of the mounting cover, a drip tube fixedly connected to the front side of the linear motor, a fixed horizontal plate fixedly connected to the bottom of the rear side of the mounting cover, support frames fixedly connected to the front and rear sides of the top of the fixed horizontal plate, a pull rod slidably connected through the top of the support frame, a clamping spring movably sleeved on the surface of the pull rod, a clamping plate fixedly connected to the bottom of the pull rod, a liquid storage mechanism fixedly connected to the top of the rear side of the inner cavity of the mounting cover, the liquid storage mechanism comprising a liquid storage tank, a drive motor bolted to the left side of the liquid storage tank, the output shaft of the drive motor penetrating into the inner cavity of the liquid storage tank and a stirring rod fixedly sleeved on its surface, and a micro-pump fixedly connected to the right side of the bottom of the inner cavity of the liquid storage tank.

[0006] Preferably, a mounting plate is fixedly connected to the front side of the linear motor, and the surface of the drip tube is fixedly connected to the mounting plate through it.

[0007] Preferably, an electric heating module is fixedly connected to the top of the base plate, and a transparent cover is movably connected to the front side of the top of the mounting cover via a hinge.

[0008] Preferably, a partition is fixedly connected to the inner cavity of the liquid storage tank, and the inlet pipe of the micro pump extends through to the left side of the partition.

[0009] Preferably, the outlet end of the micro pump is fixedly connected to a telescopic hose, and the right side of the telescopic hose passes through the storage tank and is fixedly connected to the dripping pipe.

[0010] Preferably, the top of the liquid storage tank is fixedly connected to an injection pipe, and the top of the injection pipe extends through to the top of the mounting cover.

[0011] Preferably, a control panel is fixedly connected to the front side of the base plate, and support columns are fixedly connected to the four corners of the bottom of the base plate.

[0012] Preferably, the support column includes a threaded sleeve, the bottom of which is threadedly connected to a support screw, and the bottom of the support screw is welded to a support base plate.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] 1. The drive motor rotates the stirring rod to stir the dye in the storage tank. The micro pump extracts the dye and delivers it through the telescopic hose to the dropper, which finally drips onto the specimen slide below. This avoids dye sedimentation and improves staining quality.

[0015] 2. The slide is placed between the front and rear support frames. The clamping spring releases its elastic potential energy to push the clamping plate to clamp and fix the slide. This can clamp the slide more firmly and prevent the slide from shifting and changing the staining position due to instrument shaking, thus ensuring the progress of the experimental research. Attached Figure Description

[0016] Figure 1 This is an isometric view of the structure of this utility model;

[0017] Figure 2 This is an unfolded isometric view of the transparent cover of this utility model;

[0018] Figure 3 This is the right axonometric view of the fixed horizontal plate of this utility model;

[0019] Figure 4 This is an isometric view of the interior of the mounting housing of this utility model;

[0020] Figure 5 This is an isometric view of the internal structure of the liquid storage tank of this utility model.

[0021] In the diagram: 1. Base plate; 2. Control panel; 3. Support column; 4. Injection tube; 5. Transparent cover; 6. Mounting cover; 7. Support base plate; 8. Threaded sleeve; 9. Support screw; 10. Fixed horizontal plate; 11. Liquid storage mechanism; 12. Linear motor; 13. Electric heating module; 14. Clamping spring; 15. Support frame; 16. Clamping plate; 17. Pull rod; 18. Telescopic hose; 19. Mounting plate; 20. Drip tube; 21. Drive motor; 22. Stirring rod; 23. Liquid storage tank; 24. Partition plate; 25. Miniature liquid pump. Detailed Implementation

[0022] Please see Figures 1-5 An electron microscope specimen section staining device includes a base plate 1. A mounting cover 6 is fixedly connected to the top of the base plate 1. A linear motor 12 is bolted to the middle of the rear side of the inner cavity of the mounting cover 6. By setting the linear motor 12, the drip tube 20 can be easily moved left and right, so as to drip liquid onto the glass slides on the fixed horizontal plates 10 below. The drip tube 20 is fixedly connected to the front side of the linear motor 12. A fixed horizontal plate 10 is fixedly connected to the bottom of the rear side of the mounting cover 6. Supports are fixedly connected to the front and rear sides of the top of the fixed horizontal plate 10. A support frame 15 has a pull rod 17 slidably connected through its top. A clamping spring 14 is movably sleeved on the surface of the pull rod 17. A clamping plate 16 is fixedly connected to the bottom of the pull rod 17. A liquid storage mechanism 11 is fixedly connected to the top of the rear side of the inner cavity of the mounting cover 6. The liquid storage mechanism 11 includes a liquid storage tank 23. A drive motor 21 is bolted to the left side of the liquid storage tank 23. The output shaft of the drive motor 21 passes through the inner cavity of the liquid storage tank 23 and a stirring rod 22 is fixedly sleeved on its surface. A micro liquid pump 25 is fixedly connected to the right side of the bottom of the inner cavity of the liquid storage tank 23.

[0023] Please see Figure 4 A mounting plate 19 is fixedly connected to the front side of the linear motor 12. By setting the mounting plate 19, it has the function of supporting the installation and can support the drip tube 20 to the top of the glass slide. The surface of the drip tube 20 is fixedly connected to the mounting plate 19 through it.

[0024] Please see Figure 2 An electric heating module 13 is fixedly connected to the top of the base plate 1. By setting the electric heating module 13, it has the function of heating and drying, which can make the dye dry quickly. A transparent cover 5 is movably connected to the front side of the top of the mounting cover 6 through a hinge. The transparent cover 5 is made of transparent acrylic sheet material, which makes it convenient for personnel to watch the dyeing process from the outside.

[0025] Please see Figure 5 The inner cavity of the liquid storage tank 23 is fixedly connected to a partition 24, and the inlet pipe of the micro pump 25 extends through to the left side of the partition 24.

[0026] Please see Figure 4 The outlet end of the micro pump 25 is fixedly connected to a telescopic hose 18. By setting the telescopic hose 18, it can be easily moved around with the dripping tube 20. The right side of the telescopic hose 18 passes through the storage tank 23 and is fixedly connected to the dripping tube 20.

[0027] Please see Figure 1 The top of the liquid storage tank 23 is fixedly connected to the injection pipe 4, and the top of the injection pipe 4 extends through to the top of the mounting cover 6.

[0028] Please see Figure 2 The control panel 2 is fixedly connected to the front side of the base plate 1, and the four corners of the bottom of the base plate 1 are fixedly connected to the support columns 3. By setting the support columns 3, the height of the four corners of the instrument can be adjusted. By rotating the support screw 9 forward or backward, the support height of the support screw 9 can be changed, so that the instrument can maintain balance on uneven tabletops.

[0029] Please see Figure 2 The support column 3 includes a threaded sleeve 8, and a support screw 9 is threadedly connected to the bottom of the threaded sleeve 8. A support base plate 7 is welded to the bottom of the support screw 9. By setting the support base plate 7, the contact area between the support screw 9 and the table can be increased, and the support can be more stable.

[0030] In use, connect the device to an external power source and pour the metal salt dye into the storage tank 23 through the injection tube 4 for storage. When staining sections is required, open the transparent cover 5, pull the lever 17 upwards to place the slide between the front and rear support frames 15 on the fixed horizontal plate 10, and then release the lever 17. The clamping spring 14 releases its elastic potential energy to push the clamping plate 16 to clamp and fix the slide. This can clamp the slide more firmly, prevent the instrument from shaking and causing the slide to shift and change the staining position, and ensure the progress of the experimental research. Control the drive motor 21 to work and drive the stirring rod 22 to rotate and stir the dye in the storage tank 23. Then, the micro pump 25 works to extract the dye and deliver it to the drop tube 20 through the telescopic hose 18. Finally, it drips onto the specimen section on the slide below. This can prevent the dye from settling and improve the staining quality.

[0031] In summary, this electron microscope specimen section staining device, through the coordinated use of the base plate 1, mounting cover 6, fixing cross plate 10, liquid storage mechanism 11, linear motor 12, clamping spring 14, support frame 15, clamping plate 16, pull rod 17 and telescopic hose 18, solves the problems of dye settling easily after long-term static storage, affecting staining quality, and insufficient fixation of glass slides, which affects the progress of experimental research.

Claims

1. An electron microscope specimen section staining device, comprising a base plate (1), characterized in that: A mounting cover (6) is fixedly connected to the top of the base plate (1). A linear motor (12) is bolted to the middle of the rear side of the inner cavity of the mounting cover (6). A drip tube (20) is fixedly connected to the front side of the linear motor (12). A fixed horizontal plate (10) is fixedly connected to the bottom of the rear side of the mounting cover (6). Support frames (15) are fixedly connected to the front and rear sides of the top of the fixed horizontal plate (10). A pull rod (17) is slidably connected through the top of the support frame (15). A clamp is movably sleeved on the surface of the pull rod (17). The spring (14) is held, and the bottom of the pull rod (17) is fixedly connected to the clamping plate (16). The top of the rear side of the inner cavity of the mounting cover (6) is fixedly connected to the liquid storage mechanism (11). The liquid storage mechanism (11) includes a liquid storage tank (23). The left side of the liquid storage tank (23) is connected to the drive motor (21) by bolts. The output shaft of the drive motor (21) passes through the inner cavity of the liquid storage tank (23) and a stirring rod (22) is fixedly sleeved on its surface. A micro liquid pump (25) is fixedly connected to the right side of the bottom of the inner cavity of the liquid storage tank (23).

2. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: The linear motor (12) is fixedly connected to the front side of the mounting plate (19), and the surface of the drip tube (20) is fixedly connected to the mounting plate (19) through it.

3. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: An electric heating module (13) is fixedly connected to the top of the base plate (1), and a transparent cover (5) is movably connected to the front side of the top of the mounting cover (6) via a hinge.

4. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: The inner cavity of the liquid storage tank (23) is fixedly connected to a partition (24), and the inlet pipe of the micro pump (25) extends through to the left side of the partition (24).

5. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: The outlet end of the micro pump (25) is fixedly connected to a telescopic hose (18), and the right side of the telescopic hose (18) passes through the storage tank (23) and is fixedly connected to the dripping pipe (20).

6. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: The top of the liquid storage tank (23) is fixedly connected to an injection pipe (4), and the top of the injection pipe (4) extends through to the top of the mounting cover (6).

7. The electron microscope specimen section staining apparatus according to claim 1, characterized in that: A control panel (2) is fixedly connected to the front side of the base plate (1), and support columns (3) are fixedly connected to the four corners of the bottom of the base plate (1).

8. The electron microscope specimen section staining apparatus according to claim 7, characterized in that: The support column (3) includes a threaded sleeve (8), and a support screw (9) is threadedly connected to the bottom of the threaded sleeve (8). A support base plate (7) is welded to the bottom of the support screw (9).

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