A device for making and staining blood smears of plasmodium

Abstract

The utility model relates to blood detection device field especially malaria parasite blood smear preparation dyeing device, including preparation box body and cover, the preparation box body is formed by bottom plate and four side plates, the two recesses that are symmetrical are equipped with on the bottom plate, one side plate is equipped with two hollow parts, the inner face of rest side plate all is provided with the flange, the setting height of each flange is different, the cover is covered in preparation box body, just is provided with the blowing mechanism for sending air to the accommodation cavity in the cover, this device fills in a little sample no device auxiliary operation's blank, uses the device when dyeing, adopts inverted dyeing, greatly reduces the probability that dyeing crystal adheres on the slide and smear because of vertical settlement, environmental temperature, solvent volatilization etc. , guarantees the picture quality, improves result determination reliability, can save reagent dosage, promotes smear evaporation through blowing mechanism auxiliary smear drying, greatly saves detection time.

Description

Technical Field

[0001] This utility model relates to the technical field of blood testing devices, and in particular to a device for preparing and staining malaria parasite blood smears. Background Technology

[0002] The preparation and staining of blood smears containing Plasmodium is the "gold standard" method for diagnosing malaria, playing an irreplaceable role. Observing Plasmodium within red blood cells under a microscope is the ultimate basis for confirming malaria, clearly distinguishing it from other diseases with similar symptoms. However, at border crossings, it is necessary to test imported suspected malaria patients. Current testing methods face the following difficulties: First, the testing procedure is difficult. Border crossings typically test suspected patients irregularly, but the number of tests is small. Using mass production tools would lead to reagent waste. Second, the staining quality is poor. According to the "Malaria Parasite Detection Blood Smear Microscopic Examination Method" (WS / T 569—2017), during the testing process, due to vertical settling, ambient temperature, solvent evaporation, and other factors, a large amount of staining solution crystallization is easily generated, thus reducing staining quality and affecting experimental results. Third, the testing time is long. The entire process of blood smear preparation and staining requires more than 8 hours for drying, significantly reducing passenger clearance convenience and border crossing efficiency. Utility Model Content

[0003] The present invention provides a staining device for preparing blood smears of Plasmodium to solve the aforementioned problems.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a staining device for preparing blood smears of Plasmodium, comprising a preparation box and a cover. The preparation box consists of a bottom plate and four side plates, with a accommodating cavity in the middle. The bottom plate has two symmetrical grooves on its upper side. One of the side plates has two hollowed-out portions, which correspond one-to-one with the grooves. Both hollowed-out portions are equipped with soft plugs. The inner surfaces of the remaining side plates are provided with protruding edges, each protruding edge having a different height. The cover is placed on the preparation box, and a blower mechanism for supplying air to the accommodating cavity is provided inside the cover.

[0005] Furthermore, the length and height of each of the side plates are equal.

[0006] Furthermore, the four side plates are divided into side plate a, side plate b, side plate c and side plate d, the hollow part is provided on side plate a, and each of the protruding edges is provided on the inner surface of side plate b, side plate c and side plate d respectively.

[0007] Furthermore, the convex edge of the b side plate is higher than the convex edge of the d side plate, the convex edge of the d side plate is higher than the convex edge of the c side plate, and the lengths of all the convex edges are equal.

[0008] Furthermore, an air inlet channel is provided at the top of the cover.

[0009] Furthermore, a plurality of first magnetic blocks are distributed on the upper side of the box body, and a plurality of second magnetic blocks magnetically attracted to the first magnetic blocks are distributed on the lower side of the cover body.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows: By setting up a staining device for preparing malaria parasite blood smears, this device fills the gap in the operation of small samples without auxiliary devices. When using this device, the staining is carried out by inverting the staining, which greatly reduces the probability of staining crystals adhering to the glass slide and smear due to vertical sedimentation, ambient temperature, solvent evaporation, etc., thus ensuring image quality, improving the reliability of result judgment, saving reagent consumption, and using a blower mechanism to assist in the drying of the smear, promoting the evaporation of moisture from the smear, which greatly saves detection time. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0012] Figure 2 This is a perspective view of the box body manufactured in this utility model.

[0013] Figure 3 This is a perspective view of the box body made in this utility model from another angle.

[0014] Figure 4 This is a perspective view of the cover body in this utility model.

[0015] In the diagram: 1. Box body; 2. Lid; 11. Side panel a; 12. Side panel b; 13. Side panel c; 14. Side panel d; 22. Hair blowing mechanism; 33. Soft plug; 100. Groove; 11. Hollowed-out part; 120. Raised edge; 155. First magnetic block; 200. Second magnetic block; 202. Air inlet channel. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] As shown in the attached figure, this utility model discloses a staining device for preparing blood smears of Plasmodium, comprising a preparation box 1 and a cover 2. The preparation box 1 consists of a bottom plate and four side plates, with a accommodating cavity in the middle. Two grooves 100 are symmetrically provided on the upper side of the bottom plate for placing blood smear slides. One of the side plates has two hollowed-out portions 110, which correspond one-to-one with the grooves 100. The hollowed-out portions 110 can be used for liquid outflow, and both hollowed-out portions 110 are equipped with soft plugs 33. The inner surfaces of the remaining side plates are provided with protruding edges 120, each with a different height, gradually decreasing in height, for supporting the slides at different operating stages. The cover 2 covers the preparation box 1, and the cover 2 is provided with a blower mechanism 22 for supplying air to the accommodating cavity. The blower mechanism 22 can be a fan, used to supply air to the accommodating cavity and, together with the hollowed-out portions 110, to achieve ventilation inside the device.

[0018] This invention relates to a staining device for preparing blood smears of Plasmodium. By incorporating this device, the invention fills the gap in the lack of auxiliary equipment for small-volume sample preparation and is suitable for small-scale testing. During staining, the device employs an inverted staining method, which greatly reduces the probability of staining crystals adhering to the slide and smear due to vertical settling, ambient temperature, solvent evaporation, etc., thus ensuring image quality, improving the reliability of result judgment, and saving reagent consumption. The blower mechanism 22 assists in the drying of the smear, promoting the evaporation of moisture from the smear and greatly saving testing time.

[0019] Specifically, the length and height of each side plate are equal, with the length and height preferably being 76mm*25mm.

[0020] Specifically, the four side plates are divided into side plate a 11, side plate b 12, side plate c 13 and side plate d 14. The hollow part 110 is provided on side plate a 11, and each of the protruding edges 120 is provided on the inner surface of side plate b 12, side plate c 13 and side plate d 14 respectively.

[0021] Specifically, the protruding edge 120 of the b side plate 12 is higher than the protruding edge 120 of the d side plate 14, the protruding edge 120 of the d side plate 14 is higher than the protruding edge 120 of the c side plate 13, and the lengths of each protruding edge 120 are equal.

[0022] Specifically, the top of the cover 2 is provided with an air inlet channel 202. The air inlet channel 202 and the hollow part 110 together form a ventilation channel in the accommodating cavity. Air enters from the air inlet channel 202 and exits from the hollow part 110.

[0023] Specifically, the upper side of the box body 1 is provided with a plurality of first magnetic blocks 155, and the lower side of the cover body 2 is provided with a plurality of second magnetic blocks 200 that are magnetically attracted to the first magnetic blocks 155, so as to fix the cover body 2 and make it convenient for people to open and close the cover body 2.

[0024] In use, after preparing the blood smear according to the "Method for Microscopic Examination of Blood Smears for Detection of Malaria Parasites", place the blood smear in the groove 100, cover it with the top cover plate, start the air supply mechanism, and dry it for a certain period of time, preferably 2 hours. The drying time can be adjusted appropriately according to the condition of the blood smear. After drying, take out the blood smear slide, complete the fixation and hemolysis steps according to the standard method, place the device horizontally in a container such as a white porcelain basin, add about 2.5 ml of prepared Giemsa staining solution to another groove 100, and add it to the end near the a side plate 11. Hold the long side of the blood smear slide so that the side to be stained faces downwards, first... Place one short side of the slide against the connection between the groove 100 and side plate 11. Using this as a fulcrum, slowly lower the slide until the other short side rests against the protruding edge 120 of side plate 13. At this point, insert the soft stopper 33. Due to liquid tension and fluidity, the staining solution will evenly cover the entire area of ​​the blood smear to be stained. Start timing and stain for 15 minutes. After the time is up, remove the soft stopper 33 and gently lift the blood smear slide, placing it perpendicular to the horizontal plane, allowing the remaining staining solution to drip into the staining device. Once the remaining staining solution has dripped out, hold the long side of the blood smear slide and rinse the area to be rinsed. With the slide facing vertically upwards, first place the short side of the blood smear slide firmly against the groove 100 and the upper side of the protrusion of the b-side plate 12, securing it firmly. Then, using this point as a fulcrum, slowly and gently lower the blood smear slide vertically downwards until the other short side rests against the fixing groove of the d-side plate 14. Take a dropper and sufficient double-distilled water. Use the dropper to draw up the double-distilled water and slowly drip it onto the blood smear area near the d-side plate 14, keeping the dropper tip close to the slide as you drip. Observe the smear area until excess staining solution and residue are rinsed away. Then, gently lift the blood smear slide and place it perpendicular to the horizontal plane. Allow any remaining liquid to drip into the staining apparatus. Once the remaining staining solution has dripped out, hold the blood smear slide by its long side, with the stained side facing vertically downwards. Place the short side of the slide firmly against the groove 100 and the upper side of the protrusion on side plate 12 (b), securing it firmly. Then, using this point as a fulcrum, slowly and gently lower the slide vertically downwards until the other short side rests against the fixing groove on side plate 14 (d). Slightly tilt the container 1 to allow all the liquid inside to flow out through the hole on side plate 11 (a). Then, reposition the container 1 horizontally, close the cover, and turn on the fan. Dry for 30 minutes. The drying time can be adjusted according to the condition of the blood smear. Once the blood smear is completely dry, the staining is complete. At this point, perform the microscopic examination according to the "Malaria Parasite Detection Blood Smear Microscopic Examination Method." Add a drop of cedarwood oil or a special infusion oil to the blood film and examine it under an optical microscope.

[0025] The above embodiments are preferred embodiments of this utility model. All structures similar to this utility model and equivalent changes thereof should fall within the protection scope of this utility model.

Claims

1. A staining apparatus for preparing blood smears of Plasmodium, characterized in that: The device includes a box body and a lid. The box body consists of a base plate and four side plates, with a accommodating cavity in the middle. The base plate has two symmetrical grooves on its upper side. One of the side plates has two hollowed-out portions, which correspond one-to-one with the grooves. Both hollowed-out portions are equipped with soft plugs. The inner surfaces of the remaining side plates are provided with raised edges, each raised edge having a different height. The lid is placed on the box body, and a blower mechanism for supplying air to the accommodating cavity is provided inside the lid.

2. The apparatus for preparing and staining malaria parasite blood smears according to claim 1, characterized in that: The length and height of each of the side plates are equal.

3. The apparatus for preparing and staining malaria parasite blood smears according to claim 2, characterized in that: The four side plates are divided into side plate a, side plate b, side plate c and side plate d. The hollow part is provided on side plate a, and each of the protruding edges is provided on the inner surface of side plate b, side plate c and side plate d respectively.

4. The apparatus for preparing and staining malaria parasite blood smears according to claim 3, characterized in that: The convex edge of the b side plate is higher than the convex edge of the d side plate, the convex edge of the d side plate is higher than the convex edge of the c side plate, and the lengths of all the convex edges are equal.

5. The apparatus for preparing and staining malaria parasite blood smears according to claim 1, characterized in that: The top of the cover is provided with an air inlet channel.

6. The apparatus for preparing and staining malaria parasite blood smears according to claim 1, characterized in that: The upper side of the box body is provided with a plurality of first magnetic blocks, and the lower side of the cover body is provided with a plurality of second magnetic blocks that are magnetically attracted to the first magnetic blocks.

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