A phytoplankton concentration sampling device and a phytoplankton detection system

Abstract

The utility model provides a kind of phytoplankton concentration sample preparation device and phytoplankton detection system, it is related to sample preparation equipment technical field, and the phytoplankton concentration sample preparation device in it includes sampling pump, first filter, concentration tank, reagent adding device, stirring device, liquid supplementing component and suction assembly;Sampling pump is connected with first filter;First filter is connected with concentration tank;Concentration tank is connected with conveying assembly;Reagent adding device is connected with concentration tank;Stirring device is installed on concentration tank;Liquid supplementing component is connected with concentration tank;Suction assembly includes lifting assembly and water suction pipe, and lifting assembly is connected with one end of water suction pipe;The other end of water suction pipe is inserted into concentration tank. After providing concentration tank by filtering silt to water sample by first filter, liquid supplementing component supplements clean water to concentration tank, directly obtains concentrated sample by extracting upper layer clean water through water suction pipe, and sample preparation is carried out by filter membrane method, compared with natural sedimentation, greatly reduce sample preparation time, improve sample preparation efficiency.

Description

Technical Field

[0001] This utility model relates to the field of sample preparation equipment technology, and in particular to a plankton concentration and sample preparation device and a plankton detection system. Background Technology

[0002] Currently, research on aquatic ecosystems (such as rivers, lakes, seas, reservoirs, and fishponds) generally requires sampling of the area and a series of analyses of the samples. Before analysis, the samples need to be concentrated to obtain specimens for microscopic observation. Existing concentration devices generally use sedimentation concentration methods for sample concentration. The traditional natural sedimentation concentration method involves a siphon process that is too cumbersome to operate manually, and any accidental vibration of the sample during the operation can ruin the entire study.

[0003] To address the above issues, existing technology, specifically the utility model patent with authorization announcement number CN222514505U, proposes an automatic sample concentration device. Sample liquid is added to a concentration tank 2, and after natural sedimentation for a certain period, a liquid suction device is activated to extract the supernatant from the concentration tank 2 to a suitable level. The concentrated sample flows out through the discharge port 21 and into the sample bottle 4 below. Finally, the sample bottle 4 is removed from the sampling port 12. The natural sedimentation and discharge processes require no manual intervention, ensuring a smooth natural sedimentation concentration process, simplifying the operation, and preventing sample vibration during sedimentation. However, the above patent still uses natural sedimentation for concentration, resulting in relatively low sample preparation efficiency. Utility Model Content

[0004] In view of this, the present invention proposes a plankton concentration and sample preparation device and a plankton detection system to solve the technical problem of low sample preparation efficiency caused by the natural sedimentation method mentioned in the background art.

[0005] The technical solution of this utility model is implemented as follows: In a first aspect, this utility model provides a plankton concentration and sample preparation device, comprising a sampling pump, a first filter, a concentration tank, a reagent addition device, a stirring device, a liquid replenishment assembly, and a suction assembly, wherein: The sampling pump is connected to the first filter and is used to draw the sample from the water sample collection point to the first filter. The first filter is connected to the concentration tank and is used to filter out sediment from the sample before supplying it to the concentration tank. The concentration tank is connected to a conveying assembly, which is used to convey samples to the sample container or the sample holder of the microscope assembly. The reagent adding device is connected to the concentration tank and is used to add Lugo reagent to the concentration tank; The stirring device is installed on the concentration tank; The replenishment component is connected to the concentration tank and is used to replenish the concentration tank with clean water; The suction assembly includes a lifting assembly and a water suction pipe. The lifting assembly is connected to one end of the water suction pipe and is used to move the water suction pipe along the height direction of the concentration tank. The other end of the water suction pipe extends into the concentration tank.

[0006] Based on the above technical solutions, preferably, if used for preparing shellfish samples, the plankton concentration preparation device further includes a second filter, which is disposed between the first filter and the concentration tank, for filtering out shellfish larvae and providing them to the concentration tank.

[0007] Based on the above technical solutions, preferably, the replenishment assembly includes a clean water tank, a first delivery pump, a first valve, and a backwashing unit. The first delivery pump is connected to the clean water tank and the concentration tank via pipelines, and the first valve is installed on the pipeline between the first delivery pump and the concentration tank. The backwashing unit includes a second delivery pump and a second valve. The second delivery pump is connected to the clean water tank and the second filter via pipelines, and the second valve is installed on the pipeline between the second delivery pump and the second filter. The second valve is also connected to the first filter via a pipeline for backwashing the first filter.

[0008] Based on the above technical solutions, preferably, the second filter is an ultrasonic filter, which includes a filter housing, a filter screen, and an ultrasonic unit. The filter screen is installed in the inner cavity of the filter housing, dividing it into a lower cavity and an upper cavity. The lower cavity is provided with a water inlet, which is connected to the first filter. The upper cavity is provided with a drain outlet, which is connected to the second valve. The ultrasonic unit is installed outside the filter housing and is used to perform ultrasonic vibration on the filter housing.

[0009] Based on the above technical solutions, preferably, the lifting assembly includes a slide rail, a support plate, a lead screw, a slider, and a driving component; the slide rail is mounted on the frame, and the length direction of the slide rail is parallel to the axial direction of the concentration tank; two support plates are respectively mounted on both ends of the slide rail; the lead screw is rotatably mounted on the support plate; the slider is slidably mounted on the slide rail and threadedly connected to the lead screw, and the slider is connected to the suction tube; the driving component is mounted on one of the support plates and is drivenly connected to the lead screw.

[0010] Based on the above technical solutions, preferably, a cleaning component is also included. The cleaning component includes a cleaning tank, a third transfer pump, and a third valve. The third transfer pump is connected to the cleaning tank and the concentration tank respectively, and is used to inject liquid to clean the concentration tank after the sample has been emptied. The third valve is installed on the pipeline between the third transfer pump and the concentration tank.

[0011] Based on the above technical solutions, preferably, the concentration tank is provided with a drain hole at the bottom, the conveying assembly includes a fourth conveying pump and a fourth valve, the fourth conveying pump is connected to the drain hole and the sample tank respectively through a pipeline, and the fourth valve is installed on the pipeline between the fourth conveying pump and the concentration tank.

[0012] Based on the above technical solutions, preferably, the concentration tank is provided with a sample inlet at the top, and the delivery assembly includes a sample inlet detection pipe, with the sample inlet and the sample holder of the microscope assembly connected to the two ends of the sample inlet pipe respectively.

[0013] Based on the above technical solutions, preferably, the reagent adding device includes a reagent storage tank, a fifth delivery pump, and a fifth valve. The reagent storage tank contains Lugo reagent. The fifth delivery pump is connected to the reagent storage tank and the concentration tank respectively through pipelines. The fifth valve is installed on the pipeline between the fifth delivery pump and the concentration tank.

[0014] Secondly, this utility model provides a plankton detection system, including a cabinet and a microscope assembly, as well as a plankton concentration and sample preparation device as described in the first aspect. The plankton concentration and sample preparation device and the microscope assembly are installed on the cabinet, and the concentration tank and the sample holder of the microscope assembly are connected through the conveying assembly.

[0015] The plankton concentration and sample preparation device and plankton detection system of this invention have the following advantages over the prior art: (1) After filtering the sediment from the sample through the first filter, the sample is provided to the concentration tank. The replenishment component replenishes the concentration tank with clean water until the required quantitative volume of the concentration tank is reached. The lifting component drives the water pumping pipe to move along the height direction of the concentration tank to the specified height. The other end of the water pumping pipe extends into the concentration tank. After the upper layer of clean water is directly extracted through the water pumping pipe, the conveying component on the concentration tank conveys the sample to the sample container or the sample holder of the microscope component. The sample is prepared by the membrane filtration method, which does not require natural sedimentation, greatly reduces the time required for sample preparation, and improves the efficiency of sample preparation. (2) The second filter is set between the first filter and the concentration tank to filter out the shellfish larvae and provide them to the concentration tank, thereby realizing the preparation of shellfish samples. One device can realize the preparation of algae samples and shellfish samples, thus improving the applicability of the device. (3) The backwashing unit includes a second delivery pump and a second valve. The second delivery pump is connected to the clear water tank and the second filter through pipelines. The second valve is installed on the pipeline between the second delivery pump and the second filter to backwash the shellfish larvae filtered by the second filter, so that all the shellfish larvae enter the concentration tank with the rinse water, thereby improving the accuracy of the measurement. (4) The second filter includes a filter housing, a filter screen and an ultrasonic unit. The filter screen is installed in the inner cavity of the filter housing and divides it into a lower cavity and an upper cavity. The lower cavity is provided with a water inlet, which is connected to the first filter. The upper cavity is provided with a drain outlet, which is connected to the second valve. During filtration, the liquid enters from the water inlet, is filtered by the filter screen and discharged from the drain outlet. During backwashing, the liquid enters from the drain outlet, backwashes the filter screen and then enters the concentration tank from the water inlet outlet. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a perspective view of the planktonic concentration and sample preparation device in an embodiment of the present invention; Figure 2 This is a schematic diagram illustrating the principle of the planktonic concentration and sample preparation device (algal sample) in this embodiment of the present invention. Figure 3 This is a schematic diagram of the suction assembly in an embodiment of the present utility model; Figure 4 This is a schematic diagram of the concentration tank in an embodiment of the present utility model; Figure 5 This is a schematic diagram of the stirring device in an embodiment of the present invention; Figure 6 This is a front view of the second filter in an embodiment of the present invention; Figure 7 This utility model Figure 6 AA section view in the middle; Figure 8This is a schematic diagram illustrating the principle of the planktonic concentration and sample preparation device (shellfish sample) in this embodiment of the present invention. Figure 9 This is a schematic diagram of the planktonic detection system in an embodiment of the present invention; Figure 10 This utility model Figure 9 A magnified view of part B in the middle.

[0018] Explanation of reference numerals in the attached drawings: 1-Sampling pump, 2-First filter, 3-Concentration tank, 4-Reagent addition device, 5-Stirring device, 6-Replenishment assembly, 7-Aspiration assembly, 8-Cleaning assembly, 9-Second filter, 10-Three-way valve; 100 - Cabinet, 200 - Microscope assembly, 201 - Sample holder; 31-Transfer assembly, 311-Fourth transfer pump, 312-Fourth valve, 313-Sample injection and detection pipeline, 32-Drain hole, 33-Sample inlet, 34-Suction hole; 41-Reagent storage tank; 42-Fifth transfer pump; 43-Fifth valve; 51-Stirring motor, 52-Stirring shaft, 53-Blade; 61-Clear water tank, 62-First transfer pump, 63-First valve, 64-Backwash unit, 641-Second transfer pump, 642-Second valve; 71-Lifting assembly, 711-Slide rail, 712-Support plate, 713-Lead screw, 714-Slider, 715-Driver, 72-Water suction pipe; 81-Cleaning tank, 82-Third transfer pump, 83-Third valve; 91-Filter housing, 911-Lower cavity, 912-Upper cavity, 913-Inlet, 914-Drain outlet, 915-Overflow outlet, 92-Filter screen, 93-Ultrasonic unit, 931-Ultrasonic transducer, 932-Protective cover. Detailed Implementation

[0019] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0020] Reference Figures 1-5As shown, in a first aspect embodiment of this utility model, a planktonic concentration preparation device for algal samples is proposed, comprising a sampling pump 1, a first filter 2, a concentration tank 3, a reagent addition device 4, a stirring device 5, a liquid replenishment component 6, and a suction component 7. These components are integrated and installed on a cabinet 100, wherein: The sampling pump 1 is connected to the first filter 2 and is used to draw the sample from the water sample collection point to the first filter 2; the sampling pump 1 can be a diaphragm pump. The first filter 2 is connected to the concentration tank 3 and is used to filter the sample of mud and sand before supplying it to the concentration tank 3; the first filter 2 has a 50-mesh screen and the waste liquid containing mud and sand obtained after filtration is discharged through the waste liquid pipeline. The concentration tank 3 is connected to a conveying assembly 31, which is used to convey samples to the sample container or the sample holder 201 of the microscope assembly 200. The reagent adding device 4 is connected to the concentration tank 3 and is used to add Lugo reagent to the concentration tank 3; The stirring device 5 is installed on the concentration tank 3; the stirring device 5 includes a stirring motor 51, a stirring shaft 52 and blades 53. The stirring shaft 52 is connected to the stirring motor 51 at the top end and to the blades 53 at the bottom end. The stirring motor 51 drives the stirring shaft 52 to rotate, and the blades 53 stir the liquid in the concentration tank 3. The replenishment component 6 is connected to the concentration tank 3 and is used to replenish clean water into the concentration tank 3; The suction assembly 7 includes a lifting assembly 71 and a suction pipe 72. The lifting assembly 71 is connected to one end of the suction pipe 72 and is used to move the suction pipe 72 along the height direction of the concentration tank 3. The other end of the suction pipe 72 extends into the concentration tank 3. The suction pipe 72 is a steel pipe, and its top end is connected to an external storage device for storing clean water via a flexible hose. The top of the concentration tank 3 is provided with a liquid extraction hole 34, into which the suction pipe 72 is inserted.

[0021] In this embodiment, the planktonic concentration sample preparation device filters sediment from the sample using the first filter 2 and then supplies it to the concentration tank 3. The replenishment component 6 replenishes the concentration tank 3 with clean water until the required quantitative volume is reached. The lifting component 71 moves the water pumping pipe 72 along the height direction of the concentration tank 3 to a specified height. The other end of the water pumping pipe 72 extends into the concentration tank 3, and the upper layer of clean water is directly extracted through the water pumping pipe 72. The conveying component 31 on the concentration tank 3 then conveys the sample to the sample container or the sample holder 201 of the microscope component 200. Sample preparation is performed using the membrane filtration method, eliminating the need for natural sedimentation, significantly reducing the time required for sample preparation, and improving the efficiency of sample preparation.

[0022] In some embodiments, the replenishment assembly 6 includes a clean water tank 61, a first transfer pump 62, a first valve 63, and a backwashing unit 64. The first transfer pump 62 is connected to the clean water tank 61 and the concentration tank 3 via pipelines. The first valve 63 is installed on the pipeline between the first transfer pump 62 and the concentration tank 3. The backwashing unit 64 includes a second transfer pump 641 and a second valve 642. The second transfer pump 641 is connected to the clean water tank 61 and the first filter 2 via pipelines. The second valve 642 is installed on the pipeline between the second transfer pump 641 and the first filter 2 for backwashing the first filter 2. The clean water in the clean water tank 61 is transferred to the first filter 2 by the second transfer pump 641 to backwash the first filter 2, preventing sediment from clogging the filter screen of the first filter 2, improving the service life of the first filter 2, and enhancing the reliability of the device. The first transfer pump 62 and the second transfer pump 641 can be diaphragm pumps. The clean water in the clean water tank 61 can be obtained by drawing tap water through the diaphragm pump and then filtering it through microfiltration and ultrafiltration.

[0023] In some embodiments, the lifting assembly 71 includes a slide rail 711, a support plate 712, a lead screw 713, a slider 714, and a drive member 715; the slide rail 711 is mounted on a frame, and the length direction of the slide rail 711 is parallel to the axial direction of the concentration tank 3; two support plates 712 are respectively mounted on both ends of the slide rail 711; the lead screw 713 is rotatably mounted on the support plate 712; the slider 714 is slidably mounted on the slide rail 711 and threadedly connected to the lead screw 713, and the slider 714 is connected to the suction tube; the drive member 715 is mounted on one of the support plates 712 and is drivenly connected to the lead screw 713. The drive component 715 drives the lead screw 713 to rotate, and the lead screw 713 threadedly drives the slider 714 to slide along the slide rail 711, thereby adjusting the height of the pipette. This adjusts the depth to which the bottom of the pipette extends into the liquid in the concentration tank 3. The height of the pipette can be determined based on the volume of the final concentrated sample; that is, when the pipette can no longer draw liquid from the concentration tank 3, the liquid in the concentration tank 3 at this point is the final concentrated sample. The drive component 715 can be a motor.

[0024] In some embodiments, the plankton concentration sample preparation device further includes a cleaning assembly 8, which includes a cleaning tank 81, a third transfer pump 82, and a third valve 83. The third transfer pump 82 is connected to both the cleaning tank 81 and the concentration tank 3, and is used to inject liquid to clean the concentration tank 3 after the sample has been emptied. The third valve 83 is installed on the pipeline between the third transfer pump 82 and the concentration tank 3. The cleaning solution in the cleaning tank 81 is transferred to the concentration tank 3 by the third transfer pump 82 to clean the concentration tank 3 after the sample has been emptied, facilitating the next sample preparation. This eliminates the need to disassemble the concentration tank 3 for cleaning, improving both measurement accuracy and sample preparation efficiency.

[0025] In some embodiments, the concentration tank 3 is provided with a drain hole 32 at the bottom, and the delivery assembly 31 includes a fourth delivery pump 311 and a fourth valve 312. The fourth delivery pump 311 is connected to the drain hole 32 and the sample container through pipes, and the fourth valve 312 is installed on the pipe between the fourth delivery pump 311 and the concentration tank 3. After the prepared sample is stored in the sample container, it can be transported through pipes to the sample holder 201 of the microscope assembly 200.

[0026] In other embodiments, the concentration tank 3 is provided with a sample inlet 33 at the top, and the delivery assembly 31 includes a sample inlet detection pipe 313, the two ends of which are respectively connected to the sample inlet 33 and the sample holder 201 of the microscope assembly 200. The sample in the concentration tank 3 is directly delivered to the sample holder 201 from the sample inlet 33 through the sample inlet detection pipe 313.

[0027] The flow of the two types of samples can be carried out simultaneously, or one of them can be selected.

[0028] In some embodiments, the reagent adding device 4 includes a reagent storage tank 41, a fifth delivery pump 42, and a fifth valve 43. The reagent storage tank 41 contains Lugo reagent. The fifth delivery pump 42 is connected to the reagent storage tank 41 and the concentration tank 3 via pipelines. The fifth valve 43 is installed on the pipeline between the fifth delivery pump 42 and the concentration tank 3.

[0029] The working principle of the planktonic concentration and sample preparation device in this embodiment of the utility model is as follows: the sampling pump 1 draws the sample from the water sample collection point to the first filter 2. After the first filter 2 filters the sediment from the sample, it is provided to the concentration tank 3. The replenishment component 6 replenishes the concentration tank 3 with clean water until the concentration tank 3 reaches the required quantitative volume. The lifting component 71 drives the water pumping pipe 72 to move along the height direction of the concentration tank 3 to the specified height. The other end of the water pumping pipe 72 extends into the concentration tank 3. After the upper layer of clean water is directly drawn out through the water pumping pipe 72, the sample in the concentration tank 3 is directly transported to the sample holder 201 through the sample inlet 33 through the sample inlet detection pipe 313, or the sample in the concentration tank 3 is transported to the sample tank storage through the drain hole 32 through the fourth delivery pump 311.

[0030] The second aspect of this utility model, in conjunction with... Figure 1 , Figure 3-8 A planktonic sample preparation device for shellfish samples is proposed, comprising a sampling pump 1, a first filter 2, a second filter 9, a concentration tank 3, a reagent addition device 4, a stirring device 5, a liquid replenishment assembly 6, and a suction assembly 7, wherein: The sampling pump 1 is connected to the first filter 2 and is used to draw the sample from the water sample collection point to the first filter 2; the sampling pump 1 can be a submersible pump. The first filter 2 is connected to the concentration tank 3 and is used to filter the mud and sand from the sample and then supply it to the second filter 9; the first filter 2 has a 50-mesh screen, and the waste liquid containing mud and sand obtained after filtration is discharged through the waste liquid pipeline. The second filter 9 is disposed between the first filter 2 and the concentration tank 3, and is used to filter out the shellfish larvae and provide them to the concentration tank 3; a three-way valve 10 is also provided between the second filter 9 and the first filter 2, with port a of the three-way valve 10 connected to the first filter 2, port b connected to the second filter 9, and port c connected to the concentration tank 3. During filtration, ports a and b of the three-way valve 10 are open and port c is closed; during backwashing, port a is closed and ports b and c are open. The concentration tank 3 is connected to a conveying assembly 31, which is used to convey samples to the sample container or the sample holder 201 of the microscope assembly 200. The reagent adding device 4 is connected to the concentration tank 3 and is used to add Lugo reagent to the concentration tank 3; The stirring device 5 is installed on the concentration tank 3; the stirring device 5 includes a stirring motor 51, a stirring shaft 52 and blades 53. The stirring shaft 52 is connected to the stirring motor 51 at the top end and to the blades 53 at the bottom end. The stirring motor 51 drives the stirring shaft 52 to rotate, and the blades 53 stir the liquid in the concentration tank 3. The replenishment component 6 is connected to the concentration tank 3 and is used to replenish clean water into the concentration tank 3; The suction assembly 7 includes a lifting assembly 71 and a water suction pipe 72. The lifting assembly 71 is connected to one end of the water suction pipe 72 and is used to drive the water suction pipe 72 to move along the height direction of the concentration tank 3. The other end of the water suction pipe 72 extends into the concentration tank 3.

[0031] The plankton concentration and sample preparation device in this embodiment of the invention filters out shellfish larvae through the second filter 9 and provides them to the concentration tank 3; a three-way valve 10 is also provided between the second filter 9 and the first filter 2. Port a of the three-way valve 10 is connected to the first filter 2, port b is connected to the second filter 9, and port c is connected to the concentration tank 3. During filtration, ports a and b of the three-way valve 10 are open and port c is closed; during backwashing, port a is closed and ports b and c are open, thereby realizing the preparation of shellfish samples.

[0032] In some embodiments, the replenishment assembly 6 includes a clean water tank 61, a first delivery pump 62, a first valve 63, and a backwashing unit 64. The first delivery pump 62 is connected to the clean water tank 61 and the concentration tank 3 via pipes, and the first valve 63 is installed on the pipe between the first delivery pump 62 and the concentration tank 3. The backwashing unit 64 includes a second delivery pump 641 and a second valve 642. The second delivery pump 641 is connected to the clean water tank 61 and the second filter 9 via pipes, and the second valve 642 is installed on the pipe between the second delivery pump 641 and the second filter 9. The second valve 642 is also connected to the first filter 2 via a pipe for backwashing the first filter 2. The second pump 641 delivers clean water from the clean water tank 61 to the second filter 9, backwashing the shellfish larvae filtered out by the second filter 9. This ensures that all the shellfish larvae enter the concentration tank 3 along with the rinse water, improving measurement accuracy. The second pump 641 also delivers clean water from the clean water tank 61 to the first filter 2, backwashing the first filter 2 to prevent sediment from clogging the filter screen, thus extending the service life of the first filter 2 and improving the reliability of the device. The first pump 62 and the second pump 641 can be diaphragm pumps; in this embodiment, the second valve 642 is a three-way valve 10.

[0033] In some embodiments, the second filter 9 is an ultrasonic filter, comprising a filter housing 91, a filter screen 92, and an ultrasonic unit 93. The filter screen 92 is installed in the inner cavity of the filter housing 91, dividing it into a lower cavity 911 and an upper cavity 912. The lower cavity 911 has an inlet 913 connected to the first filter 2. The upper cavity 912 has a drain outlet 914 connected to the second valve 642. The top of the upper cavity 912 also has an overflow outlet 915 for releasing pressure when the pressure exceeds a preset threshold. The ultrasonic unit 93 is installed outside the filter housing 91 for ultrasonic vibration of the filter housing 91. During filtration, liquid enters through the inlet 913, is filtered by the filter screen 92, and is discharged through the drain outlet 914. During backwashing, liquid enters through the drain outlet 914, backwashes the filter screen 92, and then exits through the outlet 913 into the concentration tank 3. The ultrasonic unit includes an ultrasonic transducer 931 and a protective cover 932. The ultrasonic transducer 931 is mounted on the filter shell 91 and located above the upper cavity 912. The protective cover 932 is connected to the top of the filter shell 91 and covers the ultrasonic transducer 931.

[0034] In some embodiments, the lifting assembly 71 includes a slide rail 711, a support plate 712, a lead screw 713, a slider 714, and a drive member 715; the slide rail 711 is mounted on a frame, and the length direction of the slide rail 711 is parallel to the axial direction of the concentration tank 3; two support plates 712 are respectively mounted on both ends of the slide rail 711; the lead screw 713 is rotatably mounted on the support plate 712; the slider 714 is slidably mounted on the slide rail 711 and threadedly connected to the lead screw 713, and the slider 714 is connected to the suction tube; the drive member 715 is mounted on one of the support plates 712 and is drivenly connected to the lead screw 713. The drive component 715 drives the lead screw 713 to rotate, and the lead screw 713 threadedly drives the slider 714 to slide along the slide rail 711, thereby adjusting the height of the pipette. This adjusts the depth to which the bottom of the pipette extends into the liquid in the concentration tank 3. The height of the pipette can be determined based on the volume of the final concentrated sample; that is, when the pipette can no longer draw liquid from the concentration tank 3, the liquid in the concentration tank 3 at this point is the final concentrated sample. The drive component 715 can be a motor.

[0035] In some embodiments, the plankton concentration sample preparation device further includes a cleaning assembly 8, which includes a cleaning tank 81, a third transfer pump 82, and a third valve 83. The third transfer pump 82 is connected to both the cleaning tank 81 and the concentration tank 3, and is used to inject liquid to clean the concentration tank 3 after the sample has been emptied. The third valve 83 is installed on the pipeline between the third transfer pump 82 and the concentration tank 3. The cleaning solution in the cleaning tank 81 is transferred to the concentration tank 3 by the third transfer pump 82 to clean the concentration tank 3 after the sample has been emptied, facilitating the next sample preparation. This eliminates the need to disassemble the concentration tank 3 for cleaning, improving both measurement accuracy and sample preparation efficiency.

[0036] In some embodiments, the concentration tank 3 is provided with a drain hole 32 at the bottom, and the delivery assembly 31 includes a fourth delivery pump 311 and a fourth valve 312. The fourth delivery pump 311 is connected to the drain hole 32 and the sample container through pipes, and the fourth valve 312 is installed on the pipe between the fourth delivery pump 311 and the concentration tank 3. After the prepared sample is stored in the sample container, it can be transported through pipes to the sample holder 201 of the microscope assembly 200.

[0037] In other embodiments, the concentration tank 3 is provided with a sample inlet 33 at the top, and the delivery assembly 31 includes a sample inlet detection pipe 313, the two ends of which are respectively connected to the sample inlet 33 and the sample holder 201 of the microscope assembly 200. The sample in the concentration tank 3 is directly delivered to the sample holder 201 from the sample inlet 33 through the sample inlet detection pipe 313.

[0038] The flow of the two types of samples can be carried out simultaneously, or one of them can be selected.

[0039] In some embodiments, the reagent adding device 4 includes a reagent storage tank 41, a fifth delivery pump 42, and a fifth valve 43. The reagent storage tank 41 contains Lugo reagent. The fifth delivery pump 42 is connected to the reagent storage tank 41 and the concentration tank 3 via pipelines. The fifth valve 43 is installed on the pipeline between the fifth delivery pump 42 and the concentration tank 3.

[0040] The working principle of the plankton concentration and sample preparation device proposed in this embodiment is as follows: The shellfish larvae are filtered out by the second filter 9 and supplied to the concentration tank 3. During filtration, ports a and b of the three-way valve 10 are open, and port c is closed. When port a of the three-way valve 10 is closed and ports b and c are open, the second delivery pump 641 delivers clean water from the clean water tank 61 to the second filter 9, backwashing the shellfish larvae filtered out by the second filter 9, so that all the shellfish larvae enter the concentration tank 3 along with the backwash water. The replenishment component 6 supplies the... The concentration tank 3 is replenished with clean water until the required quantitative volume is reached. The lifting component 71 drives the water pumping pipe 72 to move along the height direction of the concentration tank 3 to the specified height. The other end of the water pumping pipe 72 extends into the concentration tank 3. After the upper layer of clean water is directly extracted through the water pumping pipe 72, the sample in the concentration tank 3 is directly transported to the sample holder 201 through the sample inlet 33 via the sample inlet pipe 313, or the sample in the concentration tank 3 is transported to the sample container for storage through the drain hole 32 via the fourth delivery pump 311.

[0041] Based on the same concept, the third aspect of this utility model, combined with Figure 9 and Figure 10 As shown, a planktonic detection system is provided, including a cabinet 100 and a microscope assembly 200, as well as a planktonic concentration and sample preparation device as described in the first and second aspect embodiments. The planktonic concentration and sample preparation device and the microscope assembly 200 are mounted on the cabinet 100, and the concentration tank 3 and the sample holder 201 of the microscope assembly 200 are connected through the transport assembly 31.

[0042] The plankton detection system proposed in this embodiment can simultaneously detect algae and shellfish samples in water samples through a single inspection system, thereby improving detection efficiency.

[0043] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A plankton concentration and sample preparation device, characterized in that, Includes a sampling pump, a first filter, a concentration tank, a reagent addition device, a stirring device, a replenishment assembly, and a suction assembly, wherein: The sampling pump is connected to the first filter and is used to draw the sample from the water sample collection point to the first filter. The first filter is connected to the concentration tank and is used to filter out sediment from the sample before supplying it to the concentration tank. The concentration tank is connected to a conveying assembly, which is used to convey samples to the sample container or the sample holder of the microscope assembly. The reagent adding device is connected to the concentration tank and is used to add Lugo reagent to the concentration tank; The stirring device is installed on the concentration tank; The replenishment component is connected to the concentration tank and is used to replenish the concentration tank with clean water; The suction assembly includes a lifting assembly and a water suction pipe. The lifting assembly is connected to one end of the water suction pipe and is used to move the water suction pipe along the height direction of the concentration tank. The other end of the water suction pipe extends into the concentration tank.

2. The plankton concentration and sample preparation device as described in claim 1, characterized in that, If used for preparing shellfish samples, the plankton concentration preparation device further includes a second filter, which is disposed between the first filter and the concentration tank, for filtering out shellfish larvae and providing them to the concentration tank.

3. The plankton concentration and sample preparation device as described in claim 2, characterized in that, The replenishment assembly includes a clean water tank, a first delivery pump, a first valve, and a backwashing unit. The first delivery pump is connected to the clean water tank and the concentration tank via pipelines, and the first valve is installed on the pipeline between the first delivery pump and the concentration tank. The backwashing unit includes a second delivery pump and a second valve. The second delivery pump is connected to the clean water tank and the second filter via pipelines, and the second valve is installed on the pipeline between the second delivery pump and the second filter. The second valve is also connected to the first filter via a pipeline for backwashing the first filter.

4. The plankton concentration and sample preparation device as described in claim 3, characterized in that, The second filter is an ultrasonic filter, comprising a filter housing, a filter screen, and an ultrasonic unit. The filter screen is installed in the inner cavity of the filter housing, dividing it into a lower cavity and an upper cavity. The lower cavity has a water inlet connected to the first filter. The upper cavity has a drain outlet connected to the second valve. The ultrasonic unit is installed outside the filter housing and is used to perform ultrasonic vibration on the filter housing.

5. The plankton concentration and sample preparation device as described in claim 1, characterized in that, The lifting assembly includes a slide rail, a support plate, a lead screw, a slider, and a drive component; the slide rail is mounted on the frame, and its length direction is parallel to the axis of the concentration tank; two support plates are respectively mounted on both ends of the slide rail; the lead screw is rotatably mounted on the support plate; the slider is slidably mounted on the slide rail and threadedly connected to the lead screw, and the slider is connected to the water pumping pipe; the drive component is mounted on one of the support plates and is drivenly connected to the lead screw.

6. The plankton concentration and sample preparation device as described in claim 1, characterized in that, It also includes a cleaning assembly, which includes a cleaning tank, a third transfer pump, and a third valve. The third transfer pump is connected to the cleaning tank and the concentration tank respectively, and is used to inject liquid to clean the concentration tank after the sample has been emptied. The third valve is installed on the pipeline between the third transfer pump and the concentration tank.

7. The plankton concentration and sample preparation device as described in claim 1, characterized in that, The concentration tank is provided with a drain hole at the bottom. The conveying assembly includes a fourth conveying pump and a fourth valve. The fourth conveying pump is connected to the drain hole and the sample tank through a pipe. The fourth valve is installed on the pipe between the fourth conveying pump and the concentration tank.

8. The plankton concentration and sample preparation device as described in claim 1, characterized in that, The concentration tank is provided with a sample inlet at the top, and the delivery assembly includes a sample inlet detection pipe, with the sample inlet and the sample holder of the microscope assembly connected to the two ends of the sample inlet and the sample holder of the microscope assembly, respectively.

9. The plankton concentration and sample preparation device as described in claim 1, characterized in that, The reagent addition device includes a reagent storage tank, a fifth delivery pump, and a fifth valve. The reagent storage tank contains Lugo reagent. The fifth delivery pump is connected to the reagent storage tank and the concentration tank via pipelines. The fifth valve is installed on the pipeline between the fifth delivery pump and the concentration tank.

10. A plankton detection system, characterized in that, The device includes a cabinet and a microscope assembly, as well as a plankton concentration and sample preparation apparatus according to any one of claims 1-9, wherein the plankton concentration and sample preparation apparatus and the microscope assembly are mounted on the cabinet, and the concentration tank and the sample holder of the microscope assembly are connected through the transport assembly.

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