A sample analysis device and optical measurement device

Abstract

The application discloses a sample analysis device and an optical measurement device, which are used for reducing the volume of an optical measurement component and improving the convenience of integrating the optical measurement component in the sample analysis device. The sample analysis device in the application comprises a reaction component having at least one placement position for placing a reaction container; and an optical measurement component comprising a light source, a front light assembly arranged in a light path between the light source and the reaction container and used for generating a transmission light beam and a scattering light beam, and a rear light assembly used for collecting the transmission light beam deviated from an optical axis by a first angle and the scattering light beam deviated from the optical axis by a second angle, and transmitting the collected transmission light beam of the first angle and the scattering light beam of the second angle to a transmission light detector and a scattering light detector respectively, the first angle comprises ± alpha, the second angle comprises ± beta to ± gamma, and beta is greater than alpha, the transmission light detector is used for measuring the received transmission light, and the scattering light detector is used for measuring the received scattering light.

Description

Technical Field

[0001] This application relates to the field of sample measurement technology, and in particular to a sample analysis device and an optical measurement device. Background Technology

[0002] In the field of optical measurement technology, the scattering spectrometer is a common optical measuring instrument widely used in chemistry, biomedicine, environmental engineering, and other fields. Its basic principle is to obtain the sample's characteristic parameters, such as concentration and particle size, by measuring the intensity of the scattered light after the light source passes through the sample.

[0003] However, traditional scattering photometers typically require separate scattering light acquisition paths for scattered light signals and separate transmission light acquisition paths for transmitted light signals. These separate paths generally require a large amount of space, resulting in a large size for traditional scattering photometers, which is not conducive to their integration into sample analysis devices. Utility Model Content

[0004] This application provides a sample analysis device and an optical measurement device, which can simultaneously separate scattered light and transmitted light by combining a back-light component in one optical path, thereby reducing the space occupied by the scattered light path and the transmitted light path in the optical measurement component, and improving the convenience of integrating the optical measurement component in the sample analysis device.

[0005] A first aspect of this application provides a sample analysis apparatus, comprising at least:

[0006] A reaction component having at least one placement position for placing a reaction vessel and incubating the reaction liquid in the reaction vessel;

[0007] An optical measurement component is used to perform optical measurement on the incubated reaction solution to obtain reaction data of the sample. The optical measurement component includes:

[0008] A light source, used to generate an incident light beam;

[0009] A front light assembly is disposed in the optical path between the light source and the reaction container to shape the incident light beam to obtain a parallel light beam that propagates to the reaction container, so as to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container.

[0010] The rear optical assembly, positioned in the optical path of the reaction vessel away from the front optical assembly along the propagation direction of the transmitted beam, is used to collect the transmitted beam deviating from the optical axis by a first angle and the scattered beam deviating from the optical axis by a second angle, and to propagate the collected transmitted beam at the first angle and the scattered beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes... The second angle includes to And the ;

[0011] The rear optical assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a through hole in the center or a second reflector without a through hole in the center.

[0012] Alternatively, the rear optical assembly may include a third focusing lens assembly with a centrally located through-hole, and a third reflector or a second fiber optic receiver;

[0013] The transmitted light detector is used to measure the received transmitted light to obtain the transmitted light measurement data of the reaction liquid.

[0014] The scattered light detector is used to measure the received scattered light to obtain the scattered light measurement data of the reaction liquid.

[0015] As an optional embodiment, if the rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a first reflecting mirror disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the transmitted light beam and propagate the converged transmitted light beam to the through hole of the first reflecting mirror, so that the converged transmitted light beam propagates to the transmitted light detector after passing through the through hole of the first reflecting mirror.

[0016] The first focusing lens assembly is also used to converge the scattered beam and propagate the converged scattered beam to the non-through-hole of the first reflector, so that the converged scattered beam is reflected by the first reflector to the second focusing lens assembly, and the second focusing lens assembly propagates the secondary converged scattered beam to the scattered light detector.

[0017] As an optional embodiment, if the diameter of the through-hole of the first reflector is d, and the distance between the reaction container and the first focusing lens assembly is L1, then the projection d1 of the diameter d of the through-hole of the first reflector along the direction of the transmitted light spot diameter satisfies:

[0018] .

[0019] As an optional embodiment, the reaction vessel, the first focusing lens assembly, the first reflector, and the transmission light detector are coaxially arranged in the first optical path, the second focusing lens assembly and the scattering light detector are coaxially arranged in the second optical path, and the first optical path is perpendicular to the second optical path.

[0020] As an optional embodiment, the distance L2 between the reaction vessel and the transmission light detector is set between 80 mm and 100 mm, and the distance L3 between the first reflector and the scattering light detector is set between 50 mm and 80 mm.

[0021] As an optional embodiment, if the rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a second reflector disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the scattered light beam and propagate the converged scattered light beam to the second focusing lens assembly, so that the second focusing lens assembly propagates the secondary converged scattered light beam to the scattered light detector.

[0022] The first focusing lens assembly is also used to converge the transmitted beam and propagate the converged transmitted beam to the second reflector, so that the second reflector reflects the converged transmitted beam to the transmitted light detector.

[0023] As an optional embodiment, if the distance between the reaction vessel and the first focusing lens assembly is L1, and the projection dimension of the second reflecting mirror along the diameter direction of the transmitted beam spot is d2, then d2 satisfies:

[0024] .

[0025] As an optional embodiment, the first focusing lens assembly, the second reflector, the second focusing lens assembly, and the scattered light detector are coaxially arranged in the third optical path;

[0026] The transmitted light detector is disposed in the fourth optical path, and the third optical path is perpendicular to the fourth optical path.

[0027] As an optional embodiment, the distance L4 between the reaction vessel and the scattering light detector is set between 80 mm and 100 mm, and the distance L5 between the second reflector and the transmission light detector is set between 50 mm and 80 mm.

[0028] As an optional embodiment, the second reflector and the second focusing lens assembly are combined into a cemented lens;

[0029] If the second focusing lens assembly includes a single lens, then the second reflecting mirror is disposed at the center of the single lens;

[0030] If the second focusing lens assembly includes multiple lenses, then the second reflecting mirror is positioned at the center of the target lens closest to the second reflecting mirror.

[0031] As an optional embodiment, if the backlight assembly includes a first focusing lens assembly, a second focusing lens assembly, and a first optical fiber receiver disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the scattered beam and propagate the converged scattered beam to the second focusing lens assembly, so that the second focusing lens assembly propagates the secondary converged scattered beam to the scattered light detector.

[0032] The first focusing lens assembly is also used to converge the transmitted beam and propagate the converged transmitted beam to the first fiber optic receiver, so that the first fiber optic receiver receives the converged transmitted beam and propagates the converged transmitted beam to the transmission photodetector.

[0033] As an optional embodiment, the radius of curvature of the first focusing lens assembly includes 10 mm to 30 mm, and the radius of curvature of the second focusing lens assembly includes 10 mm to 30 mm.

[0034] As an optional embodiment, if the rear light assembly includes a third focusing lens assembly with a centrally located through hole and a third reflector or a second fiber optic receiver, then the third focusing lens assembly is used to converge the scattered light beam and propagate the converged scattered light beam to the scattered light detector.

[0035] The transmitted light beam propagates to the third reflecting mirror after passing through the through-hole of the third focusing lens assembly, so that the third reflecting mirror reflects the transmitted light beam to the transmission photodetector;

[0036] or,

[0037] The transmitted light beam propagates to the second fiber optic receiver after passing through the through-hole of the third focusing lens assembly, so that the second fiber optic receiver propagates the transmitted light beam to the transmission photodetector.

[0038] As an optional embodiment, if the distance between the reaction vessel and the third focusing lens assembly is L6, then the diameter d3 of the through hole in the third focusing lens assembly satisfies:

[0039] ;

[0040] The aperture D1 of the third focusing lens assembly satisfies:

[0041] .

[0042] As an optional embodiment, if the distance between the reaction vessel and the first focusing lens assembly is L1, then the aperture D2 of the first focusing lens assembly satisfies:

[0043] .

[0044] A second aspect of this application provides an optical measurement device, comprising:

[0045] A light source, used to generate an incident light beam;

[0046] A reaction vessel used to hold the reaction solution to be tested;

[0047] A front light assembly is disposed in the optical path between the light source and the reaction container to shape the incident light beam to obtain a parallel light beam that propagates to the reaction container, so as to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container.

[0048] A rear-light assembly, positioned in the optical path behind the reaction vessel along the propagation direction of the transmitted beam, is used to collect the transmitted beam deviating from the optical axis at a first angle and the scattered beam deviating from the optical axis at a second angle, and to propagate the collected transmitted beam at the first angle and the scattered beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes... The second angle includes to And the ;

[0049] The rear optical assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a through hole in the center or a second reflector without a through hole in the center.

[0050] Alternatively, the rear optical assembly may include a third focusing lens assembly with a centrally located through-hole, and a third reflector or a second fiber optic receiver;

[0051] The transmitted light detector is used to measure the received transmitted light to obtain the transmitted light measurement data of the reaction liquid.

[0052] The scattered light detector is used to measure the received scattered light to obtain the scattered light measurement data of the reaction liquid.

[0053] As can be seen from the above technical solutions, the embodiments of this application have the following advantages:

[0054] The sample analysis device in this embodiment includes: a reaction component having at least one placement position for placing a reaction container and incubating the reaction liquid in the reaction container; and an optical measurement component for performing photometric measurements on the incubated reaction liquid to obtain reaction data of the sample. The optical measurement component includes: a light source for generating an incident light beam; a front light assembly disposed in the optical path between the light source and the reaction container for shaping the incident light beam to obtain a parallel light beam propagating to the reaction container, and generating a transmitted light beam and a scattered light beam when the parallel light beam propagates to the sample in the reaction container; and a rear light assembly disposed in the optical path behind the reaction container along the propagation direction of the transmitted light beam, for collecting the transmitted light beam deviating from the optical axis by a first angle and the scattered light beam deviating from the optical axis by a second angle, and propagating the collected transmitted light beam at the first angle and the scattered light beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes... The second angle includes to ,and The rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a central through-hole or a second reflector without a central through-hole. Alternatively, the rear light assembly includes a third focusing lens assembly with a central through-hole, and a third reflector or a second fiber optic receiver. A transmission light detector is used to measure the received transmission light to obtain transmission light measurement data of the sample. A scattering light detector is used to measure the received scattering light to obtain scattering light measurement data of the sample.

[0055] Because the sample analysis device of this application embodiment can achieve the separation of scattered light and transmitted light by combining the back light component in one optical path, it reduces some optical path structure compared with the separate scattered light path and separate transmitted light path in the prior art. That is, it reduces the space occupied by the scattered light path and the transmitted light path, and improves the convenience of integrating optical measurement components in the sample analysis device. Attached Figure Description

[0056] Figure 1 This is a schematic diagram of the sample analysis device in an embodiment of this application;

[0057] Figure 2 For this application Figure 1 A schematic diagram of the functional modules in the embodiment;

[0058] Figure 3 This is a schematic diagram of one embodiment of the sample analysis device in this application.

[0059] Figure 4A This is a schematic diagram of an optical path structure of the optical measurement component 32 in an embodiment of this application;

[0060] Figure 4B This is a schematic diagram of another optical path structure of the optical measurement component 32 in the embodiments of this application;

[0061] Figure 4C This is a schematic diagram of another optical path structure of the optical measurement component 32 in the embodiments of this application;

[0062] Figure 4D This is a schematic diagram of another optical path structure of the optical measurement component 32 in the embodiments of this application;

[0063] Figure 4E This is a schematic diagram of another optical path structure of the optical measurement component 32 in the embodiments of this application. Detailed Implementation

[0064] This application provides a sample analysis device and an optical measurement device, which can simultaneously separate scattered light and transmitted light by combining a back-light component in one optical path, thereby reducing the space occupied by the scattered light path and the transmitted light path in the optical measurement component, and improving the convenience of integrating the optical measurement component in the sample analysis device.

[0065] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0066] The terms "first," "second," "third," "fourth," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments described herein can be implemented in a sequence other than that illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0067] Before detailing this application, the structure of the sample analysis device will be described first.

[0068] Please refer to Figure 1 , Figure 1 An embodiment discloses a sample analysis device, including at least one functional module 10 (or one or more functional modules 10), an input module 20, a display module 30, a memory 40, and a processor 50, which are described below.

[0069] Each functional module 10 is used to complete at least one function required in the sample analysis process. These functional modules 10 work together to complete the sample analysis and obtain the sample analysis results.

[0070] For a clearer description of functional module 10, please refer to [link / reference]. Figure 2 , Figure 2 The functional modules may include a sample component 11, a sample dispensing mechanism 12, a reagent component 13, a reagent dispensing mechanism 14, a mixing mechanism 15, a reaction component 16, and an optical measurement component 17, etc.

[0071] The sample component 11 is used to carry the sample. In some examples, the sample component 11 may include a sample delivery module (SDM) and a front-end track; in other examples, the sample component 11 may also be a sample tray, which includes multiple sample positions for placing sample tubes, and the sample tray can be rotated to the corresponding position, such as the position for the sample dispensing mechanism 12 to pick up the sample, by rotating its tray structure.

[0072] The sample dispensing mechanism 12 is used to aspirate samples and dispense them into the reaction container to be sampled. For example, the sample dispensing mechanism 12 may include a sample needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, so that the sample needle can move to aspirate the sample carried by the sample component 11, move to the reaction container to be sampled, and dispense the sample into the reaction container.

[0073] The reagent component 13 is used to hold reagents. In one embodiment, the reagent component 13 can be a reagent tray, which is arranged in a disc shape and has multiple positions for holding reagent containers. The reagent component 13 can rotate and drive the reagent containers it holds to rotate, so as to rotate the reagent containers to a specific position, such as the position where the reagent dispensing mechanism 14 picks up the reagents. The number of reagent components 13 can be one or more.

[0074] The reagent dispensing mechanism 14 is used to draw up reagents and discharge them into the reaction vessel to which the reagents are to be added. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle, which moves in two or three dimensions in space via a two-dimensional or three-dimensional driving mechanism, thereby moving the reagent needle to draw up the reagent carried by the reagent component 13, and to move to the reaction vessel to which the reagents are to be added, and to discharge the reagents into the reaction vessel.

[0075] The mixing mechanism 15 is used to mix the reaction liquid that needs to be mixed in the reaction vessel. There can be one or more mixing mechanisms 15.

[0076] The reaction component 16 has at least one placement position for placing a reaction container and incubating the reaction liquid in the reaction container. For example, the reaction component 16 can be a reaction disk, which is arranged in a disk-shaped structure and has one or more placement positions for placing reaction containers. The reaction disk can rotate and drive the reaction container in its placement position to rotate, for managing the reaction container and incubating the reaction liquid in the reaction container within the reaction disk.

[0077] The optical measurement component 17 is used to perform optical measurement on the incubated reaction solution to obtain the reaction data of the sample. For example, the optical measurement component 17 is used to measure the transmitted light beam and the scattered light beam of the reaction solution, and to measure the concentration of the reaction solution based on the transmitted light measurement data and the scattered light measurement data.

[0078] The above are some examples of functional module 10. The following will continue to describe the other components and structures in the sample analysis device.

[0079] Input module 20 is used to receive user input. Commonly, input module 20 can be a mouse and keyboard, etc. In some cases, it can also be a touch screen, which has the function of allowing users to input and display content. Therefore, in this example, input module 20 and display module 30 are integrated together.

[0080] The display module 30 can be used to display information. In some embodiments, the sample analysis device itself can integrate the display module. In other embodiments, the sample analysis device can also be connected to a computer device (e.g., a computer) and the information can be displayed through the display unit (e.g., a display screen) of the computer device. These are all within the scope of the display module 30 defined and protected herein.

[0081] Memory 40 can be volatile or persistent storage. The program stored in memory 40 may include one or more modules, each module may include a series of instruction operations on processor 50.

[0082] The processor 50 can communicate with the memory 40 and the optical measurement unit 17 to obtain the algorithm pre-stored in the memory 40, as well as the transmitted light measurement data and scattered light measurement data measured by the optical measurement unit 17, and further calculate the transmitted light measurement data and scattered light measurement data according to the algorithm to obtain the physical parameters of the reaction liquid, such as the concentration of the reaction liquid.

[0083] based on Figure 1 and Figure 2 The sample analysis device described in the above embodiment will now be described in the following embodiment of this application. Please refer to [link to relevant documentation]. Figure 3 , Figure 3 The sample analysis device includes at least:

[0084] A sample dispensing device is used to dispense samples from sample tubes into a reaction vessel;

[0085] The reagent dispensing device is used to dispense reaction reagents into the reaction container, where the sample and reaction reagents mix to form a reaction solution. The structure and function of the sample dispensing device, reagent dispensing device, and reaction container can be found in [reference needed]. Figure 2 The sample dispensing mechanism 12, reagent dispensing mechanism 14, and reaction vessel are shown in the diagram. For simplicity, the sample dispensing device, reagent dispensing device, and reaction vessel are... Figure 3 It is not shown in the middle.

[0086] Reaction component 31 (the structure of which can be found in the reference) Figure 2 The reaction component 16) and the optical measurement component 32, wherein:

[0087] The reaction component 31 has at least one placement position for placing a reaction container and incubating the reaction liquid in the reaction container. For example, the reaction component 31 can be a reaction disk, which is arranged in a disk-shaped structure and has one or more placement positions for placing reaction containers. The reaction disk can rotate and drive the reaction container in its placement position to rotate, for the purpose of managing the reaction container and incubating the reaction liquid in the reaction container within the reaction disk.

[0088] The optical measurement component 32 is used to perform photometric measurements on the incubated reaction solution to obtain the reaction data of the sample. The optical measurement component 32 includes a light source 321, a front light assembly 322, and a rear light assembly 323.

[0089] The light source 321 is used to generate an incident light beam, which can be a light beam of a preset frequency band. For example, the light source 321 in this embodiment can be selected from LEDs, halogen lamps or lasers according to the reaction liquid to be tested in the actual scene. Here, there is no specific limitation on the wavelength of the light beam of the preset frequency band emitted by the light source 321.

[0090] The front light assembly 322 is disposed in the optical path between the light source 321 and the reaction container. It is used to shape the incident light beam to obtain a parallel light beam that propagates to the reaction container, and to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container.

[0091] Specifically, in this embodiment, the front light component 322 is disposed in the optical path between the light source 321 and the reaction container, mainly for converting the incident light beam into a parallel light beam. For example, the front light component 322 can be a single convex lens or a combination of multiple convex lenses. The composition of the front light component 322 is not specifically limited here, as long as it can convert the incident light beam emitted by the light source 321 into a parallel light beam.

[0092] As an optional embodiment, if the reaction component 31 is as follows: Figure 2 As shown in the disc-shaped structure, the light source 321 and the front light assembly 322 can be coaxially arranged with the reaction vessel and disposed in the disc-shaped structure to emit a parallel light beam toward the reaction vessel.

[0093] As another alternative embodiment, in order to simplify the optical devices in the disk-shaped structure, the light source 321 and the front light assembly 322 can be arranged in a space perpendicular to the disk-shaped structure. In this embodiment, the front light assembly 322 includes a single convex lens or a combination of multiple convex lenses, as well as a reflector for reflecting a parallel light beam, wherein the reflector is used to reflect the parallel light beam into the reaction liquid of the reaction vessel.

[0094] Furthermore, in order to limit the size of the light spot propagating to the reaction vessel, the front light assembly 322 may also include an aperture disposed between the convex lens and the reaction vessel, and limit the size of the light spot propagating to the reaction vessel through a small hole in the aperture.

[0095] The rear optical component 323, positioned in the optical path of the reaction vessel away from the front optical component 322 along the propagation direction of the transmitted beam, is used to collect the transmitted beam deviating from the optical axis at a first angle and the scattered beam deviating from the optical axis at a second angle. It then propagates the collected transmitted beam at the first angle and the scattered beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes... The second angle includes to And the ;

[0096] In one embodiment, the rear optical assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a reflector 3233 or a first fiber optic receiver 3234 disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232. The reflector 3233 includes a first reflector 32331 with a central through-hole or a second reflector 32332 without a central through-hole, as well as a transmission light detector 3235 for collecting the transmitted light beam and a scattering light detector 3236 for collecting the scattered light beam. For ease of understanding... Figure 4A A schematic diagram of the optical path is given when the rear optical assembly 323 includes the first reflector 32331. Figure 4B A schematic diagram of the optical path is given when the rear optical assembly 323 includes the second reflector 32332. Figure 4C A schematic diagram of the optical path is given when the rear optical component 323 includes the first optical fiber receiver 3234.

[0097] In another embodiment, the rear optical assembly 323 includes a third focusing lens assembly 3237 with a centrally located through-hole, a third reflecting mirror 3238 or a second fiber optic receiver 3239, a transmission light detector 3235 for collecting the transmitted beam, and a scattering light detector 3236 for collecting the scattered beam. For ease of understanding... Figure 4D A schematic diagram of the optical path is given when the rear optical assembly 323 includes the third reflector 3238. Figure 4E A schematic diagram of the optical path is given when the rear optical component includes the second optical fiber receiver 3239.

[0098] Among them, for the optical path Figure 4A , 4B The process of separating the transmitted beam and the scattered beam through the rear light assembly 323, as well as measuring the transmitted beam and the scattered beam, will be described in the following embodiments and will not be repeated here.

[0099] Because the sample analysis device of this application embodiment can achieve the separation of scattered light and transmitted light by combining the back light component in one optical path, it reduces some optical path structure compared with the separate scattered light path and separate transmitted light path in the prior art. That is, it reduces the space occupied by the scattered light path and the transmitted light path, and improves the convenience of integrating optical measurement components in the sample analysis device.

[0100] The embodiments of 4A, 4B, 4C, 4D, and 4E will be described below:

[0101] I. For example Figure 4A As shown, if the rear light assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a first reflecting mirror 32331 disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232...

[0102] Specifically, targeting Figure 4A :

[0103] When the light source 321 propagates the parallel beam to the reaction vessel through the front light assembly 322, a transmitted beam and a scattered beam are generated on the side of the reaction vessel away from the front light assembly 322 along the propagation direction of the parallel beam. The transmitted beam is a portion of the parallel beam that passes through the reaction liquid in the reaction vessel, while the scattered beam is the beam generated by the interaction of the parallel beam with various components of the reaction liquid in the reaction vessel. To collect the transmitted and scattered beams, this embodiment of the application provides a rear light assembly 323 on the side of the reaction vessel away from the front light assembly 322, wherein:

[0104] The rear light assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a first reflecting mirror 32331 with a central through hole disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232;

[0105] like Figure 4A As shown, when the transmitted beam and the scattered beam are separated using the rear optical component 323, the transmitted beam and the scattered beam will overlap. In order to separate the transmitted beam and the scattered beam, this embodiment of the application collects off-axis light. Transmitted beams within the range, and off-axis to Scattered beams within the range, and set Because the light beam within a small range off the optical axis is mainly a transmitted beam, while the light beam within a large angle off the optical axis is mainly a scattered beam, this embodiment of the application collects the light beam off the optical axis through the rear optical component 323. Transmitted beams within the range, and off-axis to The scattered beam within the range can effectively separate the transmitted beam and the scattered beam, thereby improving the signal-to-noise ratio of the acquired transmitted beam and the scattered beam.

[0106] Specifically, in this embodiment, the first focusing lens assembly 3231 is used to simultaneously focus the transmitted and scattered beams of the reaction liquid in the reaction vessel. Because the transmitted and scattered beams of the reaction liquid in the reaction vessel exhibit a completely divergent tendency, after the first focusing lens assembly 3231 focuses the transmitted and scattered beams, the completely divergent transmitted and scattered beams tend to converge or become parallel. When the transmitted and scattered beams, focused by the first focusing lens assembly 3231, propagate to the first reflecting mirror 32331, because... The first reflecting mirror 32331 has a through hole at its center. Therefore, the transmitted light beam, after being focused by the first focusing lens assembly 3231, passes through the through hole at the center of the first reflecting mirror 32331 and propagates to the transmission light detector 3235 that collects the transmitted light beam. The scattered light beam, after being focused by the first focusing lens assembly 3231, is reflected by the non-through hole of the first reflecting mirror 32331 to the second focusing lens assembly 3232, so that the second focusing lens assembly 3232 refocuses the scattered light beam and the refocused scattered light beam propagates to the scattered light detector 3236.

[0107] In this embodiment, the first focusing lens assembly 3231 and the second focusing lens assembly 3232 are single convex lenses or combinations of multiple convex lenses that converge the transmitted beam and the scattered beam. The specific configuration of the first focusing lens assembly 3231 and the second focusing lens assembly 3232 is not specifically limited here.

[0108] The transmitted light detector 3235 and the scattered light detector 3236 in the embodiments of this application can be photodiodes, photomultiplier tubes or CCDs (charge-coupled devices), etc. There is no specific limitation on the type of transmitted light detector 3235 and scattered light detector 3236 here.

[0109] Furthermore, as an optional embodiment, in order to ensure that as much of the transmitted light beam as possible passes through the through-hole centrally located on the first reflecting mirror 32331, the through-hole centrally located on the first reflecting mirror 32331 can be configured as follows:

[0110] Specifically, assuming the diameter of the through-hole at the center of the first reflecting mirror 32331 is d, and the distance between the reaction vessel and the first focusing lens assembly 3231 is L1, then the projection d1 of the diameter d of the through-hole of the first reflecting mirror along the direction of the transmitted light spot diameter satisfies:

[0111] ;

[0112] because Therefore, it can be guaranteed that the deviation from the optical axis is maintained. All the transmitted light beams inside pass through the through-hole set in the center of the first reflecting mirror 32331, and Therefore, the collected data can be avoided. to The scattered beam within the range passes through the through-hole of the first reflecting mirror, thereby achieving the focusing of beams off-axis. The transmitted beam inside, and the beam off the optical axis to Precise separation of scattered beams within the range.

[0113] Furthermore, as an optional embodiment, in order to achieve The transmitted beam inside, and to All scattered light beams within the range should pass through the first focusing lens assembly 3231 as much as possible. The aperture of the first focusing lens assembly 3231 can also be set as follows:

[0114] Specifically, assuming the distance between the reaction vessel and the first focusing lens assembly 3231 is L1, and the aperture D2 of the first focusing lens assembly satisfies:

[0115] ;

[0116] Because the embodiments of this application are set Therefore, it can be deviated from the optical axis The transmitted beam inside, and the beam off the optical axis to All scattered beams within the range pass completely through the first focusing lens assembly 3231, thereby enabling the first focusing lens assembly 3231 to focus off-axis light. The transmitted beam inside, and the beam off the optical axis to Effective focusing of scattered beams within the range.

[0117] Furthermore, as another optional embodiment, in order to achieve neatness and simplicity of the optical path, the first focusing lens assembly 3231, the first reflecting mirror 32331 and the transmission light detector 3235 can be coaxially arranged in the first optical path, and the second focusing lens assembly 3232 and the scattering light detector 3236 can be coaxially arranged in the second optical path, and the first optical path is arranged perpendicular to the second optical path.

[0118] The first and second optical paths, which are arranged perpendicularly to each other, improve the neatness and simplicity of the optical paths in the optical measurement component 32.

[0119] Furthermore, as another optional embodiment, in order to achieve miniaturization of the optical measurement component 32, this embodiment further sets the radius of curvature of the first focusing lens assembly 3231 to between 10mm and 30mm, and the radius of curvature of the second focusing lens assembly 3232 to between 10mm and 30mm. When the radius of curvature of the first focusing lens assembly 3231 is set to between 10mm and 30mm, and the radius of curvature of the second focusing lens assembly 3232 is set to between 10mm and 30mm, the distance L2 between the reaction vessel and the transmission light detector 3235 can be set to between 80mm and 100mm, and the distance L3 between the first reflecting mirror 32331 and the scattering light detector 3236 can be set to between 50mm and 80mm, thereby achieving a miniaturized design of the optical measurement component 32. This miniaturized design of the optical measurement component 32 also improves the convenience of integrating the optical measurement component 32 into the sample analysis device.

[0120] II. Figure 4B As shown, if the rear light assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a second reflecting mirror 32332 disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232;

[0121] Specifically, targeting Figure 4B :

[0122] When the light source 321 propagates the parallel beam to the reaction vessel through the front light assembly 322, a transmitted beam and a scattered beam are generated on the side of the reaction vessel away from the front light assembly 322 along the propagation direction of the parallel beam. The transmitted beam is a portion of the parallel beam that passes through the reaction liquid in the reaction vessel, while the scattered beam is the beam generated by the interaction of the parallel beam with various components of the reaction liquid in the reaction vessel. To collect the transmitted and scattered beams, this embodiment of the application provides a rear light assembly 323 on the side of the reaction vessel away from the front light assembly 322, wherein:

[0123] The rear light assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a second reflecting mirror 32332 without a central through hole disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232;

[0124] like Figure 4B As shown, when the transmitted beam and the scattered beam are separated using the rear optical component 323, the transmitted beam and the scattered beam will overlap. In order to separate the transmitted beam and the scattered beam, this embodiment of the application collects off-axis light. Transmitted beams within the range, and off-axis to Scattered beams within the range, and set Because the light beam within a small range off the optical axis is mainly a transmitted beam, while the light beam within a large angle off the optical axis is mainly a scattered beam, this embodiment of the application collects the light beam off the optical axis through the rear optical component 323. Transmitted beams within the range, and off-axis to The scattered beam within the range can effectively separate the transmitted beam and the scattered beam, thereby improving the signal-to-noise ratio of the acquired transmitted beam and the scattered beam.

[0125] Specifically, in this embodiment, the first focusing lens assembly 3231 is used to simultaneously focus the transmitted beam and the scattered beam of the reaction liquid in the reaction container. Since the transmitted beam and the scattered beam of the reaction liquid in the reaction container exhibit a completely divergent trend, after the first focusing lens assembly 3231 focuses the transmitted beam and the scattered beam, the completely divergent transmitted beam and the scattered beam exhibit a convergence or parallel trend. The second reflecting mirror 32332 is used to reflect the transmitted beam after passing through the first focusing lens assembly 3231 to the transmission light detector 3235, while the scattered beam after passing through the first focusing lens assembly 3231 propagates to the second focusing lens assembly 3232, so that the second focusing lens assembly 3232 propagates the scattered beam after secondary convergence to the scattered light detector 3236.

[0126] In this embodiment, the first focusing lens assembly 3231 and the second focusing lens assembly 3232 are single convex lenses or combinations of multiple convex lenses that converge the transmitted beam and the scattered beam. The specific configuration of the first focusing lens assembly 3231 and the second focusing lens assembly 3232 is not specifically limited here.

[0127] The transmitted light detector 3235 and the scattered light detector 3236 in the embodiments of this application can be photodiodes, photomultiplier tubes or CCDs (charge-coupled devices), etc. There is no specific limitation on the type of transmitted light detector 3235 and scattered light detector 3236 here.

[0128] Furthermore, as an optional embodiment, in order to ensure that the transmitted light beam converged by the first focusing lens assembly 3231 is reflected as much as possible by the second reflecting mirror 32332 to the transmission photodetector 3235, the dimensions of the second reflecting mirror 32332 can be set as follows:

[0129] If the distance between the reaction vessel and the first focusing lens assembly 3231 is L1, and the projection size of the second reflecting mirror 32332 along the diameter direction of the transmitted beam spot is d2, then d2 satisfies:

[0130] ;

[0131] because Therefore, it can be guaranteed that the deviation from the optical axis is maintained. The transmitted light beam inside is reflected by the second reflecting mirror 32332 to the transmitted light detector 3225, while This also prevents the second reflector 32332 from receiving [the signal]. to The scattered beam within the range, thus making to The scattered beam within the range is propagated to the second focusing lens assembly 3232 as much as possible, thereby achieving the retraction of the beam off the optical axis. The transmitted beam inside, and the beam off the optical axis to Precise separation of scattered beams within the range.

[0132] Furthermore, as another optional embodiment, in order to achieve The transmitted beam inside, and to All scattered light beams within the range should pass through the first focusing lens assembly 3231 as much as possible. The aperture of the first focusing lens assembly 3231 can also be set as follows:

[0133] Specifically, assuming the distance between the reaction vessel and the first focusing lens assembly 3231 is L1, and the aperture D2 of the first focusing lens assembly satisfies:

[0134] ;

[0135] Because the embodiments of this application are set Therefore, it can be deviated from the optical axis The transmitted beam inside, and the beam off the optical axis to All scattered beams within the range pass through the first focusing lens assembly, thereby achieving focusing. Internal transmitted beam and to Effective focusing of the scattered beam within the range is equivalent to improving the signal-to-noise ratio of the transmitted and scattered beams collected by the transmitted light detector 3235 and the scattered light detector 3236.

[0136] Furthermore, as another optional embodiment, the first focusing lens assembly 3231, the second reflecting mirror 32332, the second focusing lens assembly 3232, and the scattered light detector 3236 can be coaxially arranged in the third optical path, while the transmitted light detector 3235 is arranged in the fourth optical path, and the third optical path is arranged perpendicular to the fourth optical path. This perpendicular arrangement of the third and fourth optical paths achieves the neatness and simplicity of the optical paths in the optical measurement component 32.

[0137] Furthermore, as another optional embodiment, in order to reduce the number of optical components, the second reflecting mirror 32332 and the second focusing lens assembly 3232 can be combined into a cemented lens. When the second focusing lens assembly 3232 is a single lens, the second reflecting mirror 32332 is placed at the center of the single lens. When the second focusing lens assembly 3232 includes multiple lenses, the second reflecting mirror 32332 is placed at the center of the target lens closest to the second reflecting mirror 32332. Thus, the second reflecting mirror 32332 and the second focusing lens assembly 3232 are combined into one component, reducing the number of optical components in the rear light assembly 323.

[0138] Furthermore, as another optional embodiment, in order to achieve miniaturization of the optical measurement component 32, this embodiment further sets the radius of curvature of the first focusing lens assembly 3231 to between 10mm and 30mm, and the radius of curvature of the second focusing lens assembly 3232 to between 10mm and 30mm. When the radius of curvature of the first focusing lens assembly 3231 is set to between 10mm and 30mm, and the radius of curvature of the second focusing lens assembly is set to between 10mm and 30mm, the distance L2 between the reaction vessel and the transmission light detector 3235 can be set to between 80mm and 100mm, and the distance L3 between the first reflecting mirror 32331 and the scattering light detector 3236 can be set to between 50mm and 80mm, thereby achieving a miniaturized design of the optical measurement component 32. This miniaturized design of the optical measurement component 32 also improves the convenience of integrating the optical measurement component 32 into the sample analysis device.

[0139] III. Figure 4C As shown, if the rear optical assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a first optical fiber receiver 3234 disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232;

[0140] Specifically, targeting Figure 4C :

[0141] When the light source 321 propagates the parallel beam to the reaction liquid in the reaction container through the front light assembly 322, a transmitted beam and a scattered beam are generated on the side of the reaction container away from the front light assembly 322 along the propagation direction of the parallel beam. The transmitted beam is a portion of the parallel beam that passes through the reaction liquid in the reaction container, while the scattered beam is the beam generated by the interaction of the parallel beam with various components of the reaction liquid in the reaction container. To collect the transmitted and scattered beams, this embodiment of the application provides a rear light assembly 323 on the side of the reaction container away from the front light assembly 322, wherein:

[0142] The rear optical assembly 323 includes a first focusing lens assembly 3231, a second focusing lens assembly 3232, and a first optical fiber receiver 3234 disposed between the first focusing lens assembly 3231 and the second focusing lens assembly 3232;

[0143] like Figure 4C As shown, when the transmitted beam and the scattered beam are separated using the rear optical component 323, the transmitted beam and the scattered beam will overlap. In order to separate the transmitted beam and the scattered beam, this embodiment of the application collects off-axis light. Transmitted beams within the range, and off-axis to Scattered beams within the range, and set Because the light beam within a small range off the optical axis is mainly a transmitted beam, while the light beam within a large angle off the optical axis is mainly a scattered beam, this embodiment of the application collects the light beam off the optical axis through the rear optical component 323. Transmitted beams within the range, and off-axis to The scattered beam within the range can effectively separate the transmitted beam and the scattered beam, thereby improving the signal-to-noise ratio of the acquired transmitted beam and the scattered beam.

[0144] Specifically, in this embodiment, the first focusing lens assembly 3231 is used to simultaneously focus the transmitted beam and the scattered beam of the reaction liquid in the reaction container. Because the transmitted beam and the scattered beam of the reaction liquid in the reaction container exhibit a completely divergent trend, after the first focusing lens assembly 3231 focuses the transmitted beam and the scattered beam, the completely divergent transmitted beam and the scattered beam exhibit a convergence or parallel trend. The transmitted beam focused by the first focusing lens assembly 3231 propagates to the first optical fiber receiver 3234, which receives the converged transmitted beam and propagates it to the transmission light detector 3235. The scattered beam focused by the first focusing lens assembly 3231 propagates to the second focusing lens assembly 3232, so that the second focusing lens assembly 3232 propagates the secondary converged scattered beam to the scattered light detector 3236.

[0145] As an optional embodiment, in order to ensure that the first fiber optic receiver 3234 only receives the transmitted light beam and not the scattered light beam, the numerical aperture of the first fiber optic receiver 3234 can be set as follows:

[0146] If the numerical aperture of the optical fiber in the first optical fiber receiver 3234 is NA, then set as follows: ;

[0147] Because in this embodiment of the application, the numerical aperture of the first optical fiber receiver is set to be no greater than [amount missing]. Therefore, in this embodiment, the first fiber optic receiver 3234 can only receive the transmitted light beam, and cannot receive the transmitted light beam. to The scattered beams between them thus achieve the effect of scattering beams off the optical axis The transmitted beam inside, and the beam off the optical axis to Precise separation of scattered beams within the range.

[0148] Because the embodiments of this application receive the transmitted light beam through the first optical fiber receiver 3234, and the first optical fiber receiver 3234 is connected to the transmitted light detector 3235 through optical fiber, and because of the foldability of optical fiber, the miniaturization of the optical measurement component 32 is further realized.

[0149] Furthermore, as another optional embodiment, in order to achieve The transmitted beam inside, and to The scattered light beams within the range should pass through the first focusing lens assembly 3231 as much as possible. The aperture of the first focusing lens assembly 3231 can also be set as follows:

[0150] Specifically, assuming the distance between the reaction vessel and the first focusing lens assembly 3231 is L1, and the aperture D2 of the first focusing lens assembly 3231 satisfies:

[0151] ;

[0152] Because the embodiments of this application are set Therefore, it can be deviated from the optical axis The transmitted beam inside, and the beam off the optical axis to All scattered beams within the range pass completely through the first focusing lens assembly 3231, thereby achieving [the desired effect]. The transmitted beam inside and the pair to Effective focusing of the scattered beam within the range is equivalent to improving the signal-to-noise ratio of the transmitted and scattered beams collected by the transmitted light detector 3235 and the scattered light detector 3236.

[0153] Furthermore, to further miniaturize the optical measurement component 32, the embodiments of this application can also set the radius of curvature of the first focusing lens assembly 3231 to between 10mm and 30mm, and the radius of curvature of the second focusing lens assembly 3232 to between 10mm and 30mm. This allows the distance between the reaction container and the scattering light detector 3236 to be controlled between 80mm and 100mm, and the distance between the first fiber optic receiver 3234 and the transmission light detector 3235 to be set between 50mm and 80mm. This miniaturized optical measurement component 32 provides a structural basis for integrating the optical measurement component 32 into the reaction component 31, and also improves the convenience of integrating the optical measurement component 32 into the reaction component 31.

[0154] IV. Figure 4D As shown, if the rear light assembly 323 includes a third focusing lens assembly 3237 with a central through hole and a third reflecting mirror 3238;

[0155] Specifically, targeting Figure 4D :

[0156] When the light source 321 propagates the parallel beam to the reaction vessel through the front light assembly 322, a transmitted beam and a scattered beam are generated on the side of the reaction vessel away from the front light assembly 322 along the propagation direction of the parallel beam. The transmitted beam is a portion of the parallel beam that passes through the reaction liquid in the reaction vessel, while the scattered beam is the beam generated by the interaction of the parallel beam with various components of the reaction liquid in the reaction vessel. To collect the transmitted and scattered beams, this embodiment of the application provides a rear light assembly 323 on the side of the reaction vessel away from the front light assembly 322, wherein:

[0157] The rear light assembly 323 includes a third focusing lens assembly 3237 with a central through hole and a third reflecting mirror 3238;

[0158] like Figure 4D As shown, when the transmitted beam and the scattered beam are separated using the rear optical component 323, the transmitted beam and the scattered beam will overlap. In order to separate the transmitted beam and the scattered beam, this embodiment of the application collects off-axis light. Transmitted beams within the range, and off-axis to Scattered beams within the range, and set Because the light beam within a small range off the optical axis is mainly a transmitted beam, while the light beam within a large angle off the optical axis is mainly a scattered beam, this embodiment of the application collects the light beam off the optical axis through the rear optical component 323. Transmitted beams within the range, and off-axis to The scattered beam within the range can effectively separate the transmitted beam and the scattered beam, thereby improving the signal-to-noise ratio of the acquired transmitted beam and the scattered beam.

[0159] Specifically, because the third focusing lens assembly 3237 in this embodiment has a through hole at its center, it is located in... The transmitted light beam within the range passes through the through-hole at the center of the third focusing lens assembly 3237 and propagates to the third reflecting mirror 3238, so that the third reflecting mirror 3238 reflects the transmitted light beam to the transmission photodetector 3235, while the beam located within the range... to The scattered light beam within the range is focused by the third focusing lens assembly 3237 onto the scattered light detector 3236.

[0160] In this embodiment, the transmitted light detector 3235 and the scattered light detector 3236 can be photodiodes, photomultiplier tubes, or CCDs (charge-coupled devices), etc. The types of the transmitted light detector 3235 and the scattered light detector 3236 are not specifically limited here.

[0161] Furthermore, as an optional embodiment, in order to ensure that as much of the transmitted light beam as possible passes through the through-hole centrally located in the third focusing lens assembly 3237, the through-hole centrally located in the third focusing lens assembly 3237 can be configured as follows:

[0162] If the distance between the reaction vessel and the third focusing lens assembly 3237 is L6, then the diameter d3 of the through hole in the third focusing lens assembly 3237 satisfies:

[0163] ;

[0164] And in order to make it located to The scattered light beams within the range can be focused by the third focusing lens assembly 3237. In this embodiment, the aperture of the third focusing lens assembly 3237 can also be set as follows:

[0165] If the distance between the reaction vessel and the third focusing lens assembly 3237 is L6, and the aperture of the third focusing lens assembly 3237 is D1, then D1 satisfies:

[0166] ;

[0167] Because the embodiments of this application are set Therefore, the embodiments of this application can guarantee to All scattered beams within the range can be converged by the third focusing lens assembly 3237, and the setting Then it can be guaranteed The transmitted light beam passes through the through-hole in the third focusing lens assembly 3237 and propagates to the third reflecting mirror 3238, thus avoiding... to The scattered beam within the range passes through the through-hole in the third focusing lens assembly 3237, thereby achieving the focusing of the beam off-axis. The transmitted beam inside, and the beam off the optical axis to Precise separation of scattered beams within the range.

[0168] Furthermore, as another optional embodiment, in order to achieve neatness and simplicity of the optical path, the third focusing lens assembly 3237 and the scattering light detector 3236 can be coaxially arranged in the fifth optical path, while the third reflecting mirror 3238 and the transmission light detector 3235 can be coaxially arranged in the sixth optical path, and the fifth optical path is perpendicular to the sixth optical path.

[0169] Because the fifth optical path is perpendicular to the sixth optical path in this embodiment, the optical path in the optical measurement component 32 is made neat and simple, which also provides a structural basis for integrating the optical measurement component 32 into the reaction component 31.

[0170] V. For example Figure 4E As shown, if the rear optical assembly 323 includes a third focusing lens assembly 3237 with a central through hole and a second fiber optic receiver 3239;

[0171] Specifically, targeting Figure 4E :

[0172] When the light source 321 propagates the parallel beam to the reaction vessel through the front light assembly 322, a transmitted beam and a scattered beam are generated on the side of the reaction vessel away from the front light assembly 322 along the propagation direction of the parallel beam. The transmitted beam is a portion of the parallel beam that passes through the reaction liquid in the reaction vessel, while the scattered beam is the beam generated by the interaction of the parallel beam with various components of the reaction liquid in the reaction vessel. To collect the transmitted and scattered beams, this embodiment of the application provides a rear light assembly 323 on the side of the reaction vessel away from the front light assembly 322, wherein:

[0173] The rear optical assembly 323 includes a third focusing lens assembly 3237 with a central through hole and a second fiber optic receiver 3239;

[0174] like Figure 4E As shown, when the transmitted beam and the scattered beam are separated using the rear optical component 323, the transmitted beam and the scattered beam will overlap. In order to separate the transmitted beam and the scattered beam, this embodiment of the application collects off-axis light. Transmitted beams within the range, and off-axis to Scattered beams within the range, and set Because the light beam within a small range off the optical axis is mainly a transmitted beam, while the light beam within a large angle off the optical axis is mainly a scattered beam, this embodiment of the application collects the light beam off the optical axis through the rear optical component 323. Transmitted beams within the range, and off-axis to The scattered beam within the range can effectively separate the transmitted beam and the scattered beam, thereby improving the signal-to-noise ratio of the acquired transmitted beam and the scattered beam.

[0175] Specifically, because the third focusing lens assembly 3237 in this embodiment has a through hole at its center, it is located in... The transmitted light beam within the range can pass through the through hole at the center of the third focusing lens assembly 3237 and propagate to the second fiber optic receiver 3239, so that the second fiber optic receiver 3239 will propagate the received transmitted light beam to the transmission photodetector 3235, while the beam located in... to The scattered light beam within the range is focused by the third focusing lens assembly 3237 onto the scattered light detector 3236.

[0176] In this embodiment, the transmitted light detector 3235 and the scattered light detector 3236 can be photodiodes, photomultiplier tubes, or CCDs (charge-coupled devices), etc. The types of the transmitted light detector 3235 and the scattered light detector 3236 are not specifically limited here.

[0177] Furthermore, as an optional embodiment, in order to ensure that as much of the transmitted light beam as possible passes through the through-hole centrally located in the third focusing lens assembly 3237, the through-hole centrally located in the third focusing lens assembly 3237 can be configured as follows:

[0178] If the distance between the reaction vessel and the third focusing lens assembly 3237 is L6, then the diameter d3 of the through hole in the third focusing lens assembly 3237 satisfies:

[0179] ;

[0180] And in order to make it located to The scattered light beams within the range can be focused by the third focusing lens assembly 3237. In this embodiment, the aperture of the third focusing lens assembly 3237 can also be set as follows:

[0181] If the distance between the reaction vessel and the third focusing lens assembly 3237 is L6, and the aperture of the third focusing lens assembly 3237 is D1, then D1 satisfies:

[0182] ;

[0183] Because the embodiments of this application are set Therefore, the embodiments of this application can guarantee to All scattered beams within the range can be converged by the third focusing lens assembly 3237, and the setting Then it can be guaranteed The transmitted light beam passes through the through-hole in the third focusing lens assembly 3237 and propagates to the second fiber optic receiver 3239, thus avoiding... to The scattered beam within the range passes through the through-hole in the third focusing lens assembly 3237, thereby achieving the focusing of the beam off-axis. The transmitted beam inside, and the beam off the optical axis to Precise separation of scattered beams within the range.

[0184] Because in this embodiment, the transmitted light beam is propagated to the transmission light detector 3235 through the second fiber optic receiver 3239, and the second fiber optic receiver 3239 and the transmission light detector 3235 are connected by an optical fiber, the foldability of the optical fiber achieves the simplicity of the optical path in the optical measurement component 32.

[0185] Furthermore, to ensure that the second fiber optic receiver 3239 only receives... The transmitted light beam inside, but cannot be received. to Regarding the scattered light beam within the range, this embodiment of the application can also set the numerical aperture of the optical fiber in the second optical fiber receiver 3239 as follows:

[0186] Specifically, if the numerical aperture of the optical fiber in the second optical fiber receiver 3239 is NA, then the following settings are made:

[0187] NA .

[0188] Because in this embodiment of the application, the numerical aperture of the optical fiber in the second optical fiber receiver 3239 is set to be no greater than [missing value]. Therefore, the second fiber optic receiver 3239 can only receive... The transmitted light beam inside cannot be received. to The scattered beam within the range is equivalent to improving the signal-to-noise ratio of the transmitted beam collected by the transmitted light detector 3235.

[0189] The sample analysis device and its optical measurement component in the embodiments of the application have been described in detail above. The optical measurement device in the embodiments of this application will be described in detail below:

[0190] Specifically, the optical measurement device in this application embodiment includes:

[0191] A light source, used to emit a beam of light in a preset wavelength band;

[0192] A reaction vessel used to hold the reaction solution to be tested;

[0193] The front light assembly is located in the optical path between the light source and the reaction container. It is used to shape the light beam of the preset wavelength band to obtain a parallel light beam that propagates to the reaction container, so as to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container.

[0194] The rear optical assembly, positioned in the optical path behind the reaction vessel along the propagation direction of the transmitted beam, is used to collect the transmitted beam deviating from the optical axis at a first angle and the scattered beam deviating from the optical axis at a second angle. It then propagates the collected transmitted beam at the first angle and the scattered beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes... The second angle includes to ,and ;

[0195] The rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a through hole in the center or a second reflector without a through hole in the center.

[0196] Alternatively, the rear optical assembly may include a third focusing lens assembly with a centrally located through-hole, and a third reflector or a second fiber optic receiver;

[0197] A transmission light detector is used to measure the received transmitted light to obtain transmission light measurement data of the sample.

[0198] A scattered light detector is used to measure the received scattered light to obtain the scattered light measurement data of the sample.

[0199] For a detailed description of the light source, reaction vessel, front light assembly, and rear light assembly in the embodiments of this application, please refer to [link to relevant documentation]. Figure 2 and Figure 4A , 4B The descriptions of 4C, 4D and 4E will not be repeated here.

[0200] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A sample analysis device, characterized in that, At least including: A sample dispensing device is used to dispense samples from sample tubes into a reaction vessel; A reagent dispensing device is used to dispense reaction reagents into the reaction container, wherein the sample and reaction reagents in the reaction container are mixed to form a reaction solution; A reaction component having at least one placement position for placing the reaction vessel and incubating the reaction liquid in the reaction vessel; An optical measurement component is used to perform optical measurement on the incubated reaction solution to obtain reaction data of the sample. The optical measurement component includes: A light source, used to generate an incident light beam; A front light assembly is disposed in the optical path between the light source and the reaction container to shape the incident light beam to obtain a parallel light beam that propagates to the reaction container, so as to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container. The rear light assembly is disposed in the optical path of the reaction container on the side away from the front light assembly, along the propagation direction of the transmitted light beam. It is used to collect the transmitted light beam deviating from the optical axis by a first angle and the scattered light beam deviating from the optical axis by a second angle, and to propagate the collected transmitted light beam at the first angle and the scattered light beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes ±α, the second angle includes ±β to ±γ, and β > α. The rear optical assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a through hole in the center or a second reflector without a through hole in the center. Alternatively, the rear optical assembly may include a third focusing lens assembly with a centrally located through-hole, and a third reflector or a second fiber optic receiver; The transmitted light detector is used to measure the received transmitted light to obtain the transmitted light measurement data of the reaction liquid. The scattered light detector is used to measure the received scattered light to obtain the scattered light measurement data of the reaction liquid.

2. The sample analysis device according to claim 1, characterized in that, If the rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a first reflecting mirror disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the transmitted light beam and propagate the converged transmitted light beam to the through hole of the first reflecting mirror, so that the converged transmitted light beam propagates to the transmitted light detector after passing through the through hole of the first reflecting mirror. The first focusing lens assembly is also used to converge the scattered beam and propagate the converged scattered beam to the non-through-hole of the first reflector, so that the converged scattered beam is reflected by the first reflector to the second focusing lens assembly, and the second focusing lens assembly propagates the secondary converged scattered beam to the scattered light detector.

3. The sample analysis apparatus according to claim 2, characterized in that, If the diameter of the through-hole of the first reflector is d, and the distance between the reaction container and the first focusing lens assembly is L1, then the projection d1 of the diameter d of the through-hole of the first reflector along the direction of the transmitted light spot diameter satisfies: 2L1tanα≤d1≤2L1tanβ.

4. The sample analysis apparatus according to claim 2, characterized in that, The reaction vessel, the first focusing lens assembly, the first reflector, and the transmission light detector are coaxially arranged in the first optical path, the second focusing lens assembly and the scattering light detector are coaxially arranged in the second optical path, and the first optical path is perpendicular to the second optical path.

5. The sample analysis apparatus according to claim 2, characterized in that, The distance L2 between the reaction vessel and the transmission light detector is set between 80 mm and 100 mm, and the distance L3 between the first reflector and the scattering light detector is set between 50 mm and 80 mm.

6. The sample analysis apparatus according to claim 1, characterized in that, If the rear light assembly includes a first focusing lens assembly, a second focusing lens assembly, and a second reflector disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the scattered light beam and propagate the converged scattered light beam to the second focusing lens assembly, so that the second focusing lens assembly propagates the secondary converged scattered light beam to the scattered light detector. The first focusing lens assembly is also used to converge the transmitted beam and propagate the converged transmitted beam to the second reflector, so that the second reflector reflects the converged transmitted beam to the transmitted light detector.

7. The sample analysis apparatus according to claim 6, characterized in that, If the distance between the reaction vessel and the first focusing lens assembly is L1, and the projection dimension of the second reflecting mirror along the diameter direction of the transmitted beam spot is d2, then d2 satisfies: 2L1tanα≤d2≤2L1tanβ.

8. The sample analysis apparatus according to claim 6, characterized in that, The first focusing lens assembly, the second reflector, the second focusing lens assembly, and the scattered light detector are coaxially arranged in the third optical path; The transmitted light detector is disposed in the fourth optical path, and the third optical path is perpendicular to the fourth optical path.

9. The sample analysis apparatus according to claim 6, characterized in that, The distance L4 between the reaction vessel and the scattered light detector is set between 80 mm and 100 mm, and the distance L5 between the second reflector and the transmitted light detector is set between 50 mm and 80 mm.

10. The sample analysis apparatus according to claim 6, characterized in that, The second reflecting mirror and the second focusing lens assembly are combined to form a cemented lens; If the second focusing lens assembly includes a single lens, then the second reflecting mirror is disposed at the center of the single lens; If the second focusing lens assembly includes multiple lenses, then the second reflecting mirror is positioned at the center of the target lens closest to the second reflecting mirror.

11. The sample analysis apparatus according to claim 1, characterized in that, If the backlight assembly includes a first focusing lens assembly, a second focusing lens assembly, and a first optical fiber receiver disposed between the first focusing lens assembly and the second focusing lens assembly, then the first focusing lens assembly is used to converge the scattered beam and propagate the converged scattered beam to the second focusing lens assembly, so that the second focusing lens assembly propagates the secondary converged scattered beam to the scattered light detector. The first focusing lens assembly is also used to converge the transmitted beam and propagate the converged transmitted beam to the first fiber optic receiver, so that the first fiber optic receiver receives the converged transmitted beam and propagates the converged transmitted beam to the transmission photodetector.

12. The sample analysis apparatus according to claim 5, 9, or 11, characterized in that, The radius of curvature of the first focusing lens assembly ranges from 10 mm to 30 mm, and the radius of curvature of the second focusing lens assembly ranges from 10 mm to 30 mm.

13. The sample analysis apparatus according to claim 1, characterized in that, If the rear light assembly includes a third focusing lens assembly with a central through hole, and a third reflector or a second fiber optic receiver, then the third focusing lens assembly is used to converge the scattered light beam and propagate the converged scattered light beam to the scattered light detector. The transmitted light beam propagates to the third reflecting mirror after passing through the through-hole of the third focusing lens assembly, so that the third reflecting mirror reflects the transmitted light beam to the transmission photodetector; or, The transmitted light beam propagates to the second fiber optic receiver after passing through the through-hole of the third focusing lens assembly, so that the second fiber optic receiver propagates the transmitted light beam to the transmission photodetector.

14. The sample analysis apparatus according to claim 1, characterized in that, If the distance between the reaction vessel and the third focusing lens assembly is L6, then the diameter d3 of the through hole in the third focusing lens assembly satisfies: 2L6tanα≤d3≤2L6tanβ; The aperture D1 of the third focusing lens assembly satisfies: D1≥2L6tanγ.

15. The sample analysis apparatus according to claim 1, characterized in that, If the distance between the reaction vessel and the first focusing lens assembly is L1, then the aperture D2 of the first focusing lens assembly satisfies: D2≥2L1tanγ.

16. An optical measuring device, characterized in that, include: A light source, used to generate an incident light beam; A reaction vessel used to hold the reaction solution to be tested; A front light assembly is disposed in the optical path between the light source and the reaction container to shape the incident light beam to obtain a parallel light beam that propagates to the reaction container, so as to generate a transmitted light beam and a scattered light beam when the parallel light beam propagates to the reaction liquid in the reaction container. The rear light assembly is disposed in the optical path behind the reaction vessel along the propagation direction of the transmitted light beam. It is used to collect the transmitted light beam deviating from the optical axis by a first angle and the scattered light beam deviating from the optical axis by a second angle, and to propagate the collected transmitted light beam at the first angle and the scattered light beam at the second angle to the transmitted light detector and the scattered light detector, respectively. The first angle includes ±α, the second angle includes ±β to ±γ, and β > α. The rear optical assembly includes a first focusing lens assembly, a second focusing lens assembly, and a reflector or a first fiber optic receiver disposed between the first focusing lens assembly and the second focusing lens assembly. The reflector includes a first reflector with a through hole in the center or a second reflector without a through hole in the center. Alternatively, the rear optical assembly may include a third focusing lens assembly with a centrally located through-hole, and a third reflector or a second fiber optic receiver; The transmitted light detector is used to measure the received transmitted light to obtain the transmitted light measurement data of the reaction liquid. The scattered light detector is used to measure the received scattered light to obtain the scattered light measurement data of the reaction liquid.

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