Electrode rotating buckle for protein electrophoresis

Abstract

The utility model discloses an electrode rotating buckle for protein electrophoresis, which comprises an electrophoresis box body, the two sides of the electrophoresis box body are connected with a rotating buckle structure through a connecting plate, the structure comprises an adjusting rod, an adjusting clamp arm and an adjusting cross rod, the adjusting rod penetrates through the connecting plate and is rotatably connected with the adjusting cross rod, the adjusting cross rod is movably connected with the adjusting clamp arm through a fixing rod, and the adjusting clamp arm is movably connected with a fixing seat. The adjusting rod is a threaded rod body which is in threaded engagement with the connecting plate, and a locking nut is arranged on the outer side of the connecting plate; the adjusting clamp arm is provided with an adjusting groove, and the adjusting cross rod is moved to drive it to rotate around the fixing shaft. The utility model drives the adjusting clamp arm to open and close to adapt to different electrode columns through the adjusting rod, the rotating buckle structure can rotate as a whole, and is fixed in cooperation with the locking nut, has the characteristics of strong adaptability, flexible adjustment and stable connection, and can improve the reliability and efficiency of protein electrophoresis experiments.

Description

Technical Field

[0001] This utility model provides a rotating buckle, and particularly relates to a rotating buckle for an electrode used in protein electrophoresis. Background Technology

[0002] Electrode immobilization devices for protein electrophoresis are key auxiliary components used in biochemical experiments to stably place electrode columns. Their core function is to ensure a stable electrical connection between the electrode column and the electrophoresis gel or buffer during protein electrophoresis, maintaining the continuity and uniformity of the current path, thereby ensuring the accuracy and repeatability of protein separation. This is an important foundation for the smooth conduct of electrophoresis experiments.

[0003] Existing electrode fixing devices for protein electrophoresis mostly employ fixed clamp arms or simple bolt-fastening structures. Their basic structure typically includes a mounting base connected to the electrophoresis chamber, a pair of clamp arms with a fixed or limited adjustable spacing, and a single bolt assembly for locking. These devices have significant drawbacks: firstly, the clamp arm spacing is limited or impossible to adjust, meaning they can only accommodate electrode columns of specific sizes. When dealing with electrode columns of different diameters or shapes, the entire fixing device must be replaced, resulting in poor versatility and increased experimental costs. Secondly, the fixing structure is mostly a non-rotatable rigid connection, making it difficult to flexibly adjust the angle and position of the electrode column according to experimental needs. Furthermore, the simple locking method after adjustment makes it susceptible to loosening due to slight vibrations during the experiment, affecting the stability of the electrode connection and leading to uneven electrophoretic current distribution, thus impacting the reliability of the experimental results. Utility Model Content

[0004] To address the problems existing in the prior art, this utility model provides a rotating electrode clip for protein electrophoresis, which features strong adaptability, flexible adjustment, and stable connection, effectively solving the problems of poor versatility, rigid fixing methods, and insufficient stability of existing devices.

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a rotating buckle for protein electrophoresis, comprising an electrophoresis chamber, wherein rotating buckle structures are connected to both sides of the electrophoresis chamber via connecting plates, the rotating buckle structure comprising an adjusting rod, adjusting clamp arms, and adjusting crossbars; the adjusting rod passes through the connecting plates, and one end of the adjusting rod located inside the connecting plates is rotatably connected to the adjusting crossbars, and the two ends of the adjusting crossbars are movably connected to the adjusting clamp arms via fixed rods passing through them; the ends of the adjusting clamp arms near the connecting plates are movably connected via fixed seats.

[0006] The bottom of the connecting plate is fixedly connected to the electrophoresis tank by a fixing bolt structure. The adjusting rod is a threaded rod, and the connection between the connecting plate and the adjusting rod is threaded. A locking nut is provided on the side of the connecting plate away from the adjusting clamp arm and sleeved on the outside of the adjusting rod.

[0007] The fixed base abuts against the connecting plate, and its inner sides at both ends are provided with a fixed shaft that passes through itself and one end of the adjusting clamp arm.

[0008] The adjusting clamp arm is a pair of plates symmetrically distributed on both sides of the adjusting crossbar, and has adjusting grooves corresponding to the fixed rod and distributed along its own length; when the adjusting crossbar moves horizontally, it drives the adjusting clamp arm to rotate around the fixed axis.

[0009] Each of the adjusting crossbars and the pair of adjusting crossbars is fixedly connected to a connecting rod on one side close to each other. The connecting rod is provided with a locking screw that passes through it. An electrode post body is provided between the locking screws.

[0010] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages compared with the prior art:

[0011] High adaptability: Through the threaded engagement structure between the adjusting rod and the connecting plate, and in conjunction with the adjusting crossbar driving the rotational movement of the adjusting clamp arm, the opening and closing degree of the adjusting clamp arm can be flexibly adjusted to achieve stable clamping of electrode posts of different diameters and specifications. It can meet a variety of experimental needs without changing the device, greatly improving the versatility of the device.

[0012] Flexible adjustment: The rotating buckle structure can rotate around the adjustment rod as a whole, making it easy to adjust the angle and position of the electrode column according to the experimental scenario, solving the problem of rigid fixing method of traditional device; the adjustment process is realized by thread transmission, which is simple to operate and has high adjustment accuracy.

[0013] Stable connection: The rotating buckle structure is fixed with a locking nut, and the multi-point contact between the adjusting clamp arm and the electrode post can ensure the stability of the position after adjustment and avoid loosening caused by vibration during the experiment. This ensures the continuity of the electrode connection and the uniformity of the current distribution, and improves the reliability of the experimental results.

[0014] Other advantages, objectives and features of this invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be taught from the practice of this invention. Attached Figure Description

[0015] Figure 1 This is a three-dimensional schematic diagram of the installation of a rotating buckle for protein electrophoresis according to the present invention;

[0016] Figure 2 This is a cross-sectional view of the connecting plate of the electrode rotating buckle for protein electrophoresis according to the present invention;

[0017] Figure 3This is an exploded view of the rotating snap-fit ​​structure of the electrode rotating snap-fit ​​for protein electrophoresis according to this utility model.

[0018] As shown in the figure:

[0019] 1. Electrophoresis tank body; 2. Rotary buckle structure; 11. Connecting plate; 12. Fixing base; 13. Locking nut; 21. Adjusting rod; 22. Adjusting clamp arm; 23. Adjusting crossbar; 24. Fixing rod; 25. Fixing shaft; 26. Adjusting groove; 27. Locking screw. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] It should be noted that the terms "vertical," "horizontal," "up," "down," "left," "right," and similar expressions used in this article are for illustrative purposes only and do not represent the only possible implementation.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used herein in the description of this invention is for the purpose of describing particular embodiments only and is not intended to limit the invention; the term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] like Figure 1 , 2 As shown in Figure 3, a rotating buckle for protein electrophoresis includes an electrophoresis chamber 1. The two sides of the electrophoresis chamber 1 are connected to a rotating buckle structure 2 via a connecting plate 11. The rotating buckle structure 2 includes an adjusting rod 21, an adjusting clamp arm 22, and an adjusting crossbar 23.

[0024] The adjusting rod 21 passes through the connecting plate 11, and one end of it located inside the connecting plate 11 is rotatably connected to the adjusting crossbar 23. The two ends of the adjusting crossbar 23 are movably connected to the adjusting clamp arm 22 through the fixing rod 24 passing through it. The ends of the adjusting clamp arm 22 near the connecting plate 11 are movably connected through the fixing seat 12.

[0025] The bottom of the connecting plate 11 is fixedly connected to the electrophoresis tank 1 by a fixing bolt structure. The adjusting rod 21 is a threaded rod, and the connection between the connecting plate 11 and the adjusting rod 21 is threaded. A locking nut 13 is provided on the side of the connecting plate 11 away from the adjusting clamp arm 22 and sleeved on the outside of the adjusting rod 21.

[0026] The fixed base 12 abuts against the connecting plate 11, and its inner sides at both ends are provided with a fixed shaft 25 that passes through itself and one end of the adjusting clamp arm 22; the adjusting clamp arm 22 is a pair of plates symmetrically distributed on both sides of the adjusting crossbar 23, and is provided with adjusting grooves 26 that correspond to the fixed rod 24 and are distributed along its own length. When the adjusting crossbar 23 moves horizontally, it drives the adjusting clamp arm 22 to rotate around the fixed shaft 25.

[0027] The adjusting crossbar 23 and a pair of adjusting crossbars 23 are each fixedly connected to a connecting rod on the side of each other. The connecting rod is provided with a locking screw 27 that passes through it. The electrode post body is provided between the locking screws 27.

[0028] When using this device, first rotate the adjusting rod 21 according to the specifications of the electrode post. Since the adjusting rod 21 is threadedly engaged with the connecting plate 11, the adjusting rod 21 will move axially and drive the adjusting crossbar 23 to move horizontally. The fixing rods 24 at both ends of the adjusting crossbar 23 slide in the adjusting groove 26 of the adjusting clamp arm 22, causing the adjusting clamp arm 22 to rotate around the fixed shaft 25, thereby adjusting the opening and closing degree of the adjusting clamp arm 22 to adapt to the size of the electrode post. After placing the electrode post between the locking screws 27, the electrode post is further fixed by rotating the locking screws 27. If it is necessary to adjust the angle of the electrode post, the locking nut 13 can be loosened, the rotating buckle structure 2 can be rotated to a suitable position, and then the locking nut 13 can be tightened to abut against the connecting plate 11 to complete the overall fixation.

[0029] When using this device for protein electrophoresis experiments, a stable DC power supply is required from the main electrophoresis unit. The main unit is connected to the electrode column body of this device via electrode wires to form a closed circuit. The electrophoresis gel plate must be placed in the buffer tank inside the electrophoresis chamber beforehand. The buffer solution must be added precisely to the tank until it covers the gel plate using a pipette. A timer is also needed to control the electrophoresis time during the experiment. After the experiment, the protein separation bands must be observed using a UV transilluminator. The adjusting rod and adjusting clamp arm of this device are made of 304 stainless steel to ensure structural strength and chemical corrosion resistance. The connecting plate and fixing seat are made of polytetrafluoroethylene to enhance resistance to chemical reagents such as buffer solutions. The locking nut and locking screw are made of high-strength nylon to prevent short circuits when in contact with the electrode column. The adjusting crossbar and fixing shaft are made of aluminum alloy to reduce the overall weight while ensuring a stable connection.

[0030] Specifically, during the installation phase of this device, the connecting plate must first be securely connected to the pre-set mounting holes in the electrophoresis tank by tightening the bottom fixing bolts with an Allen wrench. During this process, a level can be used to calibrate the connecting plate to ensure it is level, preventing subsequent tilting of the electrode columns. Before installing the electrode column body, the contact area of ​​the locking screw must be cleaned with anhydrous ethanol. Then, strip the insulation layer from one end of the electrode wire and crimp it to the electrode column body's wiring terminal. The other end should be connected to the positive and negative output interfaces of the electrophoresis instrument and the wiring nut tightened. For buffer preparation, Tris-glycine buffer powder should be accurately measured and mixed with deionized water using a volumetric flask. After stirring with a glass rod until completely dissolved, the pH should be adjusted to 8.3 using a pH meter. Then, the solution should be poured along the buffer tank wall of the electrophoresis tank. Slowly inject the buffer solution to the designated graduation line. Before starting the experiment, use a multimeter in resistance mode to check the continuity of the circuit between the electrode column and the electrophoresis apparatus. After confirming that there is no open circuit, turn on the power of the electrophoresis apparatus and set the electrophoresis parameters to 120V and 30mA according to the experimental plan. At the same time, turn on the magnetic stirrer to keep the buffer solution circulating at a low speed to ensure uniform temperature in the tank. During the process, monitor the buffer solution temperature in real time through the temperature sensor. When the temperature exceeds 25℃, start the built-in cooling system of the electrophoresis apparatus to cool it down. After the experiment, turn off the power of the electrophoresis apparatus and disconnect the electrode leads. Then loosen the locking screw and take out the electrode column body. Rinse the adjusting arm, adjusting rod and other parts that have been in contact with the buffer solution with deionized water. After drying, turn the adjusting rod back to the initial position. Finally, put the dust cover on the electrophoresis tank to complete the equipment storage.

[0031] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A rotating electrode clip for protein electrophoresis, comprising an electrophoresis chamber (1), characterized in that: The electrophoresis tank (1) has a rotating buckle structure (2) connected to both sides by a connecting plate (11). The rotating buckle structure (2) includes an adjusting rod (21), an adjusting clamp (22), and an adjusting crossbar (23). The adjusting rod (21) passes through the connecting plate (11), and one end of it located inside the connecting plate (11) is rotatably connected to the adjusting crossbar (23). The two ends of the adjusting crossbar (23) are movably connected to the adjusting clamp arm (22) through the fixing rod (24) passing through itself. The adjusting clamp (22) is movably connected to the end of the connecting plate (11) via a fixed seat (12).

2. The electrode rotating clip for protein electrophoresis according to claim 1, characterized in that: The bottom of the connecting plate (11) is fixedly connected to the electrophoresis tank (1) by a fixing bolt structure. The adjusting rod (21) is a threaded rod, and the connection between the connecting plate (11) and the adjusting rod (21) is threaded. The side of the connecting plate (11) away from the adjusting clamp arm (22) is provided with a locking nut (13) sleeved on the outside of the adjusting rod (21).

3. The electrode rotating clip for protein electrophoresis according to claim 1, characterized in that: The fixed seat (12) abuts against the connecting plate (11), and its inner sides at both ends are provided with a fixed shaft (25) that passes through itself and one end of the adjusting clamp (22).

4. The electrode rotating clip for protein electrophoresis according to claim 3, characterized in that: The adjusting clamp (22) is a pair of plates symmetrically distributed on both sides of the adjusting crossbar (23), and has adjusting grooves (26) that correspond to the fixing rod (24) and are distributed along its own length. When the adjusting bar (23) moves horizontally, it drives the adjusting clamp (22) to rotate around the fixed axis (25).

5. The electrode rotating clip for protein electrophoresis according to claim 1, characterized in that: The adjusting crossbar (23) and the pair of adjusting crossbars (23) are each fixedly connected to a connecting rod on the side that is close to each other, and the connecting rod is provided with a locking screw (27) that passes through itself. An electrode post body is provided between the locking screws (27).

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