A thymidine kinase cell cycle assay kit

Abstract

The utility model relates to analysis kit technical field, and disclose a kind of thymidine kinase cell cycle analysis kit, including reagent box main part and sealing assembly;The reagent box main part is equipped with sample hole, and the sealing assembly is set on sample hole;The sealing assembly includes loading shaft and hole dense spare, and the loading shaft is detachably installed on sample hole, and the bottom of loading shaft is integrally formed with open mouth, and the hole dense spare is installed in open mouth, and the rotating member in loading shaft is rotatably installed on the hole dense spare, and the hollow shaft that is attached to the top of loading shaft is installed on the rotating member;Flexible sealing element is installed in the hollow shaft.The utility model realizes the injection and uniform distribution of sample solution by hollow shaft and hole disc structure, avoids impurity interference, improves detection accuracy, and simultaneously, using rotating member stirs sample solution, prevents accumulation blockage, and guarantees detection to carry out smoothly.

Description

Technical Field

[0001] This invention relates to the field of analytical reagent kit technology, and in particular to a thymidine kinase cell cycle analysis kit. Background Technology

[0002] Thymidine kinase is an important enzyme that plays a crucial role in cell cycle regulation. It is mainly involved in the synthesis of intracellular deoxythymidine monophosphate and is an important enzyme for cell proliferation and DNA synthesis. In normal cells, the expression level of thymidine kinase is relatively low, but its expression increases significantly during the S phase of the cell cycle to meet the needs of DNA synthesis during cell proliferation. The activity of thymidine kinase is closely related to the cell proliferation state. Therefore, it has important application value in cell cycle analysis, tumor cell proliferation monitoring, and cell damage assessment. In addition, changes in the concentration of thymidine kinase in the blood can also serve as a biomarker of cell proliferation and damage, providing important evidence for the diagnosis and monitoring of related diseases.

[0003] The thymidine kinase cell cycle analysis kit is a fluorescence immunochromatographic assay tool used to quantitatively detect the concentration of thymidine kinase in human serum, thereby analyzing cell cycle status. The kit mainly consists of a test card and an ID card. The test card comprises a test strip and an ABS card casing. The test strip contains a nitrocellulose membrane, a fluorescent pad, absorbent paper, and a PVC board. The nitrocellulose membrane is coated with thymidine kinase monoclonal antibody and goat anti-chicken IgY, and the fluorescent pad contains fluorescently labeled thymidine kinase monoclonal antibody and fluorescently labeled chicken IgY. During detection... The sample solution is dropped into the sample well of the reagent kit. Thymidine kinase in the sample binds to the fluorescently labeled antibody and is chromatographically ascended under capillary effect. It is captured by the antibody on the detection line (T line). The fluorescence signal intensity reflects the concentration of thymidine kinase. At the same time, chicken IgY on the fluorescent pad binds to goat anti-chicken IgY on the control line (C line) to ensure the normal operation of the detection system. The signal intensity of the T line and C line is detected by a dry fluorescence immunoassay analyzer. The concentration of thymidine kinase is calculated by combining the standard curve on the ID card, thereby realizing the analysis and detection of cell cycle status.

[0004] During testing, the sample solution needs to be dropped into the sample well of the kit. However, before the sample is added, the sample well is usually in an open state and directly exposed to the air. Dust, microorganisms and other impurities in the air can easily enter the sample well. These foreign substances may interfere with the normal reaction between the sample and the reagent, affecting the accuracy of the test results.

[0005] To address the aforementioned issues, this application proposes a thymidine kinase cell cycle analysis kit. Utility Model Content

[0006] Based on the technical problems existing in the background art, this utility model proposes a thymidine kinase cell cycle analysis kit.

[0007] This invention provides a thymidine kinase cell cycle analysis kit, comprising a kit body and a sealing component;

[0008] The reagent kit body has a sample dispensing hole, and the sealing component is disposed on the sample dispensing hole;

[0009] The sealing assembly includes a loading shaft and a densely packed perforated component. The loading shaft is detachably mounted on the sample feeding port. An opening is integrally formed at the bottom of the loading shaft. The densely packed perforated component is installed inside the opening. A rotating component located inside the loading shaft is rotatably mounted on the densely packed perforated component. A hollow shaft that fits against the top of the loading shaft is mounted on the rotating component.

[0010] A flexible seal is installed inside the hollow shaft, and an opening is integrally formed on the top of the hollow shaft. A cover plate is hinged to the hollow shaft and covers the opening.

[0011] Preferably, the perforated component includes a perforated plate, which is fixed inside the opening, and the perforated plate has densely arranged perforations.

[0012] Preferably, the rotating component includes a rotating rod, which is rotatably mounted at the center of the top surface of the perforated plate. The top of the rotating rod is equipped with a plurality of circumferentially arranged crossbars, all of which are connected to the inner wall of the hollow shaft.

[0013] Preferably, the bottom end of the rotating rod is equipped with a plurality of circumferentially arranged pusher plates, and the pusher plates are in contact with the top surface of the perforated plate.

[0014] Preferably, the flexible seal includes a first magnetic ring, a second magnetic ring, and a sealing membrane. The first magnetic ring is fixed inside the hollow shaft, the second magnetic ring is magnetically adsorbed onto the first magnetic ring, and the sealing membrane is fixed inside the second magnetic ring.

[0015] Preferably, a first magnetic ring is fixed to the top of the sample feeding hole, and a second magnetic ring, which is magnetically connected to the first magnetic ring, is installed at the bottom of the loading shaft.

[0016] The above-mentioned technical solution of this utility model has the following beneficial technical effects:

[0017] 1. Load the sample solution into the syringe, then open the cover plate at the top of the hollow shaft. Pass the syringe needle through the sealing membrane inside the hollow shaft, and then inject the sample solution into the hollow shaft through the syringe. The sample solution can be diverted to the sample well through the densely arranged perforations on the perforated plate for testing. The above design avoids the sample solution from directly contacting the open sample well, effectively isolating dust, microorganisms and other impurities in the air from entering the sample well, thereby reducing the interference of these foreign substances on the normal reaction between the sample and the reagent, and improving the accuracy of the test results. At the same time, the sample solution enters the sample well through diversion, which can make the sample solution evenly distributed, making the test results more accurate.

[0018] 2. When the sample solution is placed on the perforated plate, the hollow shaft can be rotated, causing the rotating component connected to the hollow shaft to rotate on the perforated plate. When the rotating component rotates, the sample solution on the perforated plate can be stirred by the pusher plate on the rotating component. This process not only makes the sample solution more uniformly mixed, avoiding detection deviations caused by uneven distribution of components in the sample solution, but also allows the sample solution to pass smoothly through the orifice, preventing the sample solution from accumulating or clogging the orifice, ensuring that the sample solution can flow smoothly and evenly to the first sample loading well, thereby ensuring the smooth progress of the detection process. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of a thymidine kinase cell cycle analysis kit proposed in this utility model.

[0020] Figure 2 This is a schematic diagram showing the disassembled structure of the sealing component and the main body of the reagent kit of this utility model;

[0021] Figure 3 This is a schematic diagram of the top structure of the sealing assembly of this utility model;

[0022] Figure 4 This is a schematic diagram of the bottom structure of the sealing assembly of this utility model;

[0023] Figure 5 This is an internal sectional view of the loading shaft and hollow shaft of this utility model;

[0024] Figure 6 This is a schematic diagram of the mating structure between the perforated component and the rotating component of this utility model;

[0025] Figure 7 This is a schematic diagram of the structure of the flexible sealing element of this utility model.

[0026] Reference numerals: 1. Main body of the reagent kit; 101. Sample dispensing well; 102. First magnetic ring plate; 2. Sealing assembly; 21. Loading shaft; 211. Second magnetic ring plate; 22. Dense perforated component; 221. Perforated plate; 222. Leakage hole; 23. Rotating component; 231. Rotating rod; 232. Crossbar; 233. Pusher plate; 24. Hollow shaft; 25. Flexible sealing component; 251. First magnetic ring; 252. Second magnetic ring; 253. Sealing membrane; 26. Cover plate. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings. It should be understood that these descriptions are merely exemplary and not intended to limit the scope of this utility model. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of this utility model.

[0028] like Figure 1-7 As shown, the present invention proposes a thymidine kinase cell cycle analysis kit, which includes a kit body 1 and a sealing component 2.

[0029] The reagent kit body 1 has a sample dispensing hole 101, and the sealing component 2 is disposed on the sample dispensing hole 101;

[0030] The sealing assembly 2 includes a loading shaft 21 and a hole-dense component 22. The loading shaft 21 is detachably mounted on the sample feeding port 101. An opening is integrally formed at the bottom of the loading shaft 21. The hole-dense component 22 is installed in the opening. A rotating component 23 located inside the loading shaft 21 is rotatably mounted on the hole-dense component 22. A hollow shaft 24 that fits against the top of the loading shaft 21 is mounted on the rotating component 23.

[0031] A flexible seal 25 is installed inside the hollow shaft 24. An opening is integrally formed on the top of the hollow shaft 24, and a cover plate 26 is hinged to the hollow shaft 24 and covers the opening.

[0032] When no sample is being added, the cover plate 26 closes onto the opening at the top of the hollow shaft 24. The flexible seal 25 further seals the internal space within the hollow shaft 24, effectively preventing dust, microorganisms, and other impurities from entering the loading shaft 21 and the sample dispensing hole 101, thus avoiding interference with subsequent testing. When testing is required, the sample solution is loaded into the syringe, then the cover plate 26 at the top of the hollow shaft 24 is opened, and the syringe needle is inserted through the flexible seal 25 within the hollow shaft 24 to inject the sample. Inside shaft 21, the sample solution is diverted through the porous element 22 before entering the sample dispensing port 101. When the sample solution is diverted on the porous element 22, the hollow shaft 24 can be rotated. The hollow shaft 24 drives the rotating element 23 to rotate on the porous element 22. The rotating element 23 can agitate and push the sample, so that the sample can pass smoothly through the porous element 22 and enter the sample dispensing port 101. This design not only ensures the sealing of the sample dispensing port 101 before dispensing, but also enables the smooth and uniform dispensing of the sample, improving the accuracy and reliability of the detection.

[0033] In a specific embodiment, the perforated component 22 includes a perforated plate 221, which is fixed inside the opening. The perforated plate 221 has densely arranged perforations 222. When the sample solution enters from above the loading shaft 21, it will first come into contact with the perforated plate 221. The sample solution will be divided into multiple small streams by the perforations 222 on the perforated plate 221, thereby achieving sample diversion. This diversion method can make the sample enter the sample loading hole 101 more evenly, avoid the sample from accumulating locally in the sample loading hole 101, and thus allow the sample solution to fully contact and react with the reagents in the main body of the reagent kit 1, reduce the deviation of the detection results caused by uneven sample distribution, and improve the accuracy of detection.

[0034] In a specific embodiment, the rotating component 23 includes a rotating rod 231. The rotating rod 231 is rotatably mounted on the middle of the top surface of the perforated plate 221. Multiple circumferentially arranged crossbars 232 are mounted on the top of the rotating rod 231. The multiple crossbars 232 are all connected to the inner wall of the hollow shaft 24. Multiple circumferentially arranged pusher plates 233 are mounted on the bottom of the rotating rod 231, and the pusher plates 233 are in contact with the top surface of the perforated plate 221.

[0035] When it is necessary to rotate the rotating component 23, simply rotate the hollow shaft 24. The hollow shaft 24 drives the rotating rod 231 to rotate through multiple circumferentially arranged crossbars 232 connected to the inner wall. The rotating rod 231 rotates in the middle of the top surface of the perforated plate 221. During the rotation of the rotating rod 231, the pusher plate 233 will rotate together with the rotating rod 231. Since the pusher plate 233 is in contact with the top surface of the perforated plate 221, the pusher plate 233 can push the sample solution on the top surface of the perforated plate 221 when it rotates. For samples that may flow slowly or have a tendency to accumulate on the surface of the perforated plate 221, the pusher plate 233 can push them towards the drain hole 222, ensuring that the sample can enter the sample dispensing hole 101 through the drain hole 222 in a timely manner, effectively preventing the sample from accumulating on the perforated plate 221 and causing blockage. At the same time, the pushing action of the pusher plate 233 can further enhance the stirring effect of the sample, making the sample components more uniformly mixed, reducing the adverse effects of uneven sample components on the test results, and improving the accuracy of the test.

[0036] In a specific embodiment, the flexible seal 25 includes a first magnetic ring 251, a second magnetic ring 252, and a sealing film 253. The first magnetic ring 251 is fixed inside the hollow shaft 24, the second magnetic ring 252 is magnetically adsorbed onto the first magnetic ring 251, and the sealing film 253 is fixed inside the second magnetic ring 252. In the unsampled state, the second magnetic ring 252 is magnetically adsorbed onto the first magnetic ring 251, and the sealing film 253 fixed inside the second magnetic ring 252 can tightly seal the inside of the hollow shaft 24, preventing external dust, microorganisms, and other impurities from entering the loading shaft 21 and the sample inlet 10. In step 1, to ensure the cleanliness of the internal environment of the reagent kit, when sample needs to be added, the syringe needle is inserted through the sealing membrane 253. After the sample is added, the syringe is removed, and the sealing membrane 253 can return to its original shape under its own elasticity, continuing to maintain the sealing state. If the sealing membrane 253 is damaged, since the second magnetic ring 252 and the first magnetic ring 251 are magnetically attracted together, the second magnetic ring 252 can be easily removed together with the damaged sealing membrane 253, and a new second magnetic ring 252 with the sealing membrane 253 can be replaced. The operation is simple and convenient, ensuring that the flexible sealing element 25 always has a good sealing effect.

[0037] In a specific embodiment, a first magnetic ring plate 102 is fixed to the top of the sample loading hole 101, and a second magnetic ring plate 211 magnetically connected to the first magnetic ring plate 102 is installed at the bottom of the loading shaft 21. When installing the sealing assembly 2, simply align the second magnetic ring plate 211 at the bottom of the loading shaft 21 with the first magnetic ring plate 102 at the top of the sample loading hole 101. Utilizing the magnetic attraction between the two, the loading shaft 21 can be quickly installed on the sample loading hole 101. The installation process is simple and does not require complex tools. At the same time, the magnetic connection method ensures the tightness of the connection between the loading shaft 21 and the sample loading hole 101, effectively preventing the loading shaft 21 from shifting or falling off due to vibration or accidental contact during the detection process. This ensures the sealing effect of the sealing assembly 2 on the sample loading hole 101. When it is necessary to disassemble the sealing assembly 2, simply apply a certain external force to overcome the magnetic attraction between the first magnetic ring plate 102 and the second magnetic ring plate 211, and the loading shaft 21 can be removed from the sample loading hole 101, facilitating the cleaning of the entire sealing assembly 2.

[0038] The specific working principle of this utility model is as follows:

[0039] During the sample addition operation, the cover plate 26 is opened, and the syringe needle containing the sample solution is passed through the sealing membrane 253 in the flexible seal 25. Due to its flexible properties, the sealing membrane 253 fits tightly against the needle, reducing the entry of impurities. Then, the sample solution is injected into the hollow shaft 24. The sample solution flows into the loading shaft 21 and contacts the perforated plate 221 in the perforated plate 22. If the sample does not flow smoothly or accumulates on the perforated plate 221, the hollow shaft 24 is rotated. The hollow shaft 24 drives the rotating rod 231 to rotate in the middle of the top surface of the perforated plate 221 through the crossbar 232 connected to the inner wall. The pusher plate 233 at the bottom of the rotating rod 231 rotates with the rotating rod 231, agitating and pushing the sample, so that the sample is evenly distributed and passes smoothly through the dense perforations 222 on the perforated plate 221, realizing sample diversion.

[0040] After the sample is spun out, it is evenly fed into the sample well 101 of the main body 1 of the reagent kit, and the cell cycle status can be analyzed and detected. After the detection is completed, if cleaning or maintenance is required, external force can be applied to separate the second magnetic ring 211 of the loading shaft 21 from the first magnetic ring 102 of the sample well 101, and the sealing assembly 2 can be removed for appropriate processing. If the sealing film 253 is damaged, the second magnetic ring 252 can be removed and replaced with a second magnetic ring 252 with a new sealing film 253.

[0041] It should be understood that the above-described specific embodiments of this utility model are merely illustrative or explanatory of the principles of this utility model, and do not constitute a limitation thereof. Therefore, any modifications, equivalent substitutions, improvements, etc., made without departing from the spirit and scope of this utility model should be included within the protection scope of this utility model.

Claims

1. A thymidine kinase cell cycle analysis kit, characterized in that, The kit includes the main body (1) and the sealing component (2); The reagent kit body (1) has a sample dispensing hole (101), and the sealing component (2) is disposed on the sample dispensing hole (101); The sealing assembly (2) includes a loading shaft (21) and a perforated component (22). The loading shaft (21) is detachably mounted on the sample feeding hole (101). An opening is integrally formed at the bottom of the loading shaft (21). The perforated component (22) is installed inside the opening. A rotating component (23) located inside the loading shaft (21) is rotatably mounted on the perforated component (22). A hollow shaft (24) that fits against the top of the loading shaft (21) is mounted on the rotating component (23). A flexible seal (25) is installed inside the hollow shaft (24). An opening is integrally formed on the top of the hollow shaft (24). A cover plate (26) is hinged to the hollow shaft (24) and covers the opening.

2. The thymidine kinase cell cycle analysis kit according to claim 1, characterized in that, The perforated component (22) includes a perforated plate (221), which is fixed inside the opening and has densely arranged perforations (222) on it.

3. The thymidine kinase cell cycle analysis kit according to claim 2, characterized in that, The rotating component (23) includes a rotating rod (231). The rotating rod (231) is rotatably mounted on the middle of the top surface of the perforated plate (221). Multiple circumferentially arranged crossbars (232) are mounted on the top of the rotating rod (231). The multiple crossbars (232) are all connected to the inner wall of the hollow shaft (24).

4. The thymidine kinase cell cycle analysis kit according to claim 3, characterized in that, The bottom end of the rotating rod (231) is equipped with a plurality of circumferentially arranged pusher plates (233), and the pusher plates (233) are in contact with the top surface of the perforated plate (221).

5. The thymidine kinase cell cycle analysis kit according to claim 1, characterized in that, The flexible seal (25) includes a first magnetic ring (251), a second magnetic ring (252) and a sealing membrane (253). The first magnetic ring (251) is fixed inside the hollow shaft (24), the second magnetic ring (252) is magnetically adsorbed on the first magnetic ring (251), and the sealing membrane (253) is fixed inside the second magnetic ring (252).

6. The thymidine kinase cell cycle analysis kit according to claim 1, characterized in that, The top of the sample feeding hole (101) is fixed with a first magnetic ring (102), and the bottom of the loading shaft (21) is fitted with a second magnetic ring (211) that is magnetically connected to the first magnetic ring (102).

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