A test tube rack for resuscitating cryogenic tubes

Abstract

The utility model discloses a test -tube rack for the recovery cryopreservation tube, including the support handle that constitutes by the body and the handle, and the body lower part has the threaded section, and the body is equipped with the pressure plate that can be along the body axial activity from top to bottom, the limiting block, the upper position board, the water level adjusting block, the lower position board, the cushion block and the bottom plate, and the first, second test -tube setting hole of corresponding respectively are seted up on the upper and lower position board, and the bottom plate supports the cryopreservation tube bottom end. This test -tube rack simple structure, high -efficient safety and can hold simultaneously a plurality of cryopreservation tubes, can solve the operation confusion, uneven heating and easy pollution etc. of the existing cryopreservation tube recovery process, and can be according to different cryopreservation tube size and one recovery quantity flexible matching design, applicable to a variety of using environment, realizes a plurality of cryopreservation tubes synchronization, evenly, clean recovery.

Description

Technical Field

[0001] This utility model belongs to the field of biological experimental auxiliary equipment technology, and in particular relates to a test tube rack for reviving cryopreserved tubes. Background Technology

[0002] Cryopreservation and thawing are widely used in biomedical research, clinical treatment, and biopharmaceuticals. Cell cryopreservation is a technique that preserves cells for a long period of time by placing them in a low-temperature environment (usually liquid nitrogen at -196°C), while thawing is the process of restoring cryopreserved cells to room temperature and restoring their activity.

[0003] Cryopreservation and thawing are crucial for cell preservation and recovery, involving steps such as the use of cryoprotectants, programmed cooling, and rapid thawing. Despite challenges such as ice crystal damage and osmotic pressure changes, optimizing cryopreservation and thawing conditions can effectively improve cell viability, thus making this technology valuable for applications in multiple fields.

[0004] Currently, the resuscitation process mostly involves manually removing cryovials from the liquid nitrogen environment and quickly placing them into a 37°C constant-temperature water bath to rapidly revive the cells. In medical and biological experimental research, this process requires the cryovials to be fully submerged in water and to be shaken periodically to ensure even heating; at the same time, care must be taken to ensure that the water does not exceed the cryovial cap to prevent contamination when the cap is opened, which could affect the cells inside. However, many problems still arise during the actual resuscitation process:

[0005] 1. When multiple cryopreserved tubes need to be thawed at the same time, it is easy to become flustered and confused.

[0006] 2. During thawing, the cryovials need to be shaken, but there is a risk that the cryovials may fall into the water bath while being shaken.

[0007] 3. If the cap of the cryovial gets wet, it may contaminate cells when the cap is opened. This is also possible.

[0008] Therefore, there is an urgent need for a reliable device that can provide clamping during the thawing of cryopreservation tubes. Utility Model Content

[0009] To address the problems existing in the prior art, the purpose of this utility model is to provide a test tube rack that is simple in structure, efficient and safe, and can hold multiple cryopreservation tubes simultaneously. This rack can solve the problems of chaotic operation, uneven heating and easy contamination in the existing cryopreservation tube thawing process. The test tube rack can be flexibly designed according to different cryopreservation tube sizes and the number of tubes thawing at one time, and is suitable for various usage environments, enabling the synchronous, uniform and clean thawing of multiple cryopreservation tubes.

[0010] To achieve the above and other related objectives, the present invention adopts the following technical solution:

[0011] This utility model provides a test tube rack for reviving cryopreserved tubes, including a support handle. The support handle includes a body and a grip. The grip is fixed to the top of the body, and the lower part of the body is provided with a threaded section.

[0012] The main body is provided with a pressure plate, a limiting block, an upper plate, a water level adjusting block, a lower plate, a pad block, and a bottom plate that can move along the axial direction of the main body from top to bottom;

[0013] The limiting block, water level adjusting block, pad block and base plate are all threadedly fixed to the threaded section, so that the upper plate and the lower plate are limited and fixed, and the pressure plate can slide up and down along the axis of the body.

[0014] The limiting block, water level adjusting block, pad block and base plate are all provided with threaded holes at their center positions; the pressure plate, upper plate and lower plate are all provided with center holes at their center positions; the upper plate and lower plate are also provided with a number of first test tube placement holes and second test tube placement holes for vertical penetration of cryopreservation tubes, and the positions of the openings correspond one to one; the outer edge of the base plate covers the outermost edge of all test tube placement holes to support the bottom end of the cryopreservation tube.

[0015] As a preferred technical solution, the length of the pad is determined according to the height of the cryopreservation tube to be thawed.

[0016] As a preferred technical solution, the length of the water level regulating block is determined according to the water level range during the shaking of the cryopreservation tube during revival.

[0017] Furthermore, the position of the lower plate on the rod is determined based on the cell liquid level in the cryopreservation tube to be thawed.

[0018] As a preferred technical solution, the threaded hole has an internal thread that matches the external thread of the threaded segment. The limiting block, water level adjusting block, pad block and base plate are threadedly fixed to the body through the threaded hole, so that the upper plate located between the limiting block and the water level adjusting block and the lower plate located between the water level adjusting block and the pad block are both limited and fixed on the body.

[0019] As a preferred technical solution, the central hole is circular and its inner diameter is larger than the outer diameter of the body, so that the pressure plate, upper plate, and lower plate can be sleeved on the body and slide up and down along the axial direction of the body through the central hole. The upper plate is limited by the limiting block and the water level adjusting block, and the lower plate is limited by the water level adjusting block and the pad block.

[0020] As a preferred technical solution, the first test tube placement holes are distributed in a ring shape on the upper plate, and the second test tube placement holes are distributed in a ring shape on the lower plate.

[0021] As a preferred technical solution, the base plates are all circular plates, and the radius of the base plate is not less than the distance from the outermost point of each test tube placement hole to the center of the lower plate.

[0022] As a further preferred technical solution, the upper part of the body is provided with a protrusion, and a relief groove is provided at the edge of the central hole on the pressure plate. The pressure plate moves along the axial direction of the body through the relief groove until it moves above the protrusion, and is temporarily fixed to the upper part of the body through the protrusion.

[0023] Furthermore, the protrusion is located near the handle.

[0024] As described above, the present invention has the following beneficial effects:

[0025] (1) The present invention provides a test tube rack for thawing cryopreservation tubes. Through a number of first test tube placement holes and second test tube placement holes provided on the upper plate and the lower plate, and supported by the base plate, multiple cryopreservation tubes can be clamped at the same time, realizing the thawing of multiple tubes at one time, avoiding the operational chaos caused by manual handling, and improving the thawing efficiency and safety.

[0026] (2) The present invention provides a test tube rack for reviving cryopreservation tubes. By adjusting the position of the upper plate and the lower plate through the pad and the water level adjustment block, it can ensure that the cryopreservation tube body is completely immersed in a 37°C water bath and the cap is above the liquid surface during the shaking process, so that the cells in the tube are heated evenly, while preventing water from contaminating the cap and tube opening, and ensuring the cleanliness of the cells.

[0027] (3) The test tube rack of this utility model for reviving cryopreservation tubes adopts a modular assembly structure. The upper plate and lower plate with corresponding test tube placement holes can be quickly replaced according to different cryopreservation tube diameters and the number of tubes to be revised at one time. It is suitable for various cryopreservation tube specifications, meets the needs of various experimental scenarios, and is simple, practical and inexpensive. Attached Figure Description

[0028] Figure 1 This is a cross-sectional view of the test tube rack in an embodiment of this utility model.

[0029] Figure 2 This is a schematic diagram of the test tube rack after the cryopreservation tubes are installed in an embodiment of this utility model.

[0030] Figure 3 This is a schematic diagram of the resuscitation process using a test tube rack in an embodiment of this utility model.

[0031] The specific explanations of the reference numerals in the attached drawings are as follows: 1. Support handle; 11. Grip; 12. Protrusion; 2. Pressure plate; 22. Relief groove; 3. Limiting block; 4. Upper plate; 41. First test tube placement hole; 5. Water level adjustment block; 6. Lower plate; 61. Second test tube placement hole; 7. Pad block; 8. Base plate; 9. Cryopreservation tube. Detailed Implementation

[0032] To better understand the purpose, structure, and function of this utility model, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. When describing some technical features of the main line, some alternative solutions of the technical features are interspersed as branches. Since these alternative solutions can be clearly expressed in words, no additional drawings are provided.

[0033] In the description of this utility model, it should be noted that the positional relationships indicated by terms such as "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" are based on the positional relationships shown in the accompanying drawings and are only for the convenience of describing the embodiments of this utility model and simplifying the description. They are not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific direction, and therefore should not be construed as a limitation of this utility model.

[0034] Example 1

[0035] like Figure 1 and Figure 2 As shown, this embodiment provides a test tube rack for reviving cryopreserved tubes, including a support handle 1. The support handle 1 includes a body and a grip 11. The grip 11 is fixed to the top of the body. The body has a threaded section and a protrusion 12. The threaded section is located at the lower part of the body, and the protrusion 12 is fixed to the upper part of the body. In this embodiment, the protrusion 12 is fixed near the grip 11. The threaded section on the body has, from top to bottom, a pressure plate 2, a limiting block 3, an upper plate 4, a water level adjusting block 5, a lower plate 6, a pad 7, and a base plate 8, which can move along the axial direction of the body to adjust their own position.

[0036] In this embodiment, threaded holes are provided at the center positions of the limiting block 3, the water level adjusting block 5, the pad block 7, and the base plate 8. The threaded holes have internal threads that match the external threads of the threaded segments. The limiting block 3, the water level adjusting block 5, the pad block 7, and the base plate 8 are threadedly fixed to the body through the threaded holes, thereby limiting and fixing the upper plate 4 located between the limiting block 3 and the water level adjusting block 5 and the lower plate 6 located between the water level adjusting block 5 and the pad block 7 to the body.

[0037] Meanwhile, a central hole is provided at the center of each of the pressure plate 2, the upper plate 4, and the lower plate 6. The central hole is circular and its inner diameter is larger than the outer diameter of the body, so that the pressure plate 2, the upper plate 4, and the lower plate 6 can be fitted onto the body and slide up and down along the axial direction of the body through the central hole. The upper plate 4 is limited by the limiting block 3 and the water level adjusting block 5, and the lower plate 6 is limited by the water level adjusting block 5 and the pad block 7.

[0038] In this embodiment, a clearance groove 22 is provided at the edge of the central hole on the pressure plate 2. The clearance groove 22 has a contour shape that matches the cross-section of the protrusion 12, and the contour size of the clearance groove 22 is slightly larger than the cross-sectional size of the protrusion 12 to allow the protrusion 12 to pass freely. Specifically, when the pressure plate 2 moves to the protrusion 12, the clearance groove 22 is aligned with the protrusion 12 by rotation so that the pressure plate 2 passes over the protrusion 12. Then, the pressure plate 2 is placed on the protrusion 12 by rotation again, thereby temporarily fixing the pressure plate 2 on the upper part of the body. This facilitates the subsequent batch placement of cryopreservation tubes 9 and allows a single person to quickly complete the transfer of cryopreservation tubes 9 to the test tube rack of this utility model. It effectively reduces the operation time of exposing the cryopreservation tubes 9 to room temperature, thereby ensuring that the cell revival process of the cryopreservation tubes 9 is basically completed under the constant temperature state of the water bath, improving the cell survival rate.

[0039] The length of the pad 7 is related to the length of the test tube to be thawed, and the length of the water level regulating block 5 is related to the water level to be controlled during test tube thaw. Specifically, the length of the pad 7 is determined according to the height of the cryopreservation tube 9 to be thawed, and the length of the water level regulating block 5 is determined according to the water level range to be prepared in the water bath when the cryopreservation tube 9 is shaken for thaw. In other words, the water level regulating block 5 is used to control the water level of the cryopreservation tube 9 when it is shaken in the water bath, so that the water level in the water bath is always between the upper plate 4 and the lower plate 6 when the cryopreservation tube 9 is shaken, so as to ensure that the water level does not exceed the upper plate 4. This avoids water contamination of the cryopreservation tube 9 cap and ensures that the cryopreservation tube 9 is fully immersed in the water bath to achieve rapid cell thaw. At this time, the position of the upper plate 4 on the rod is the highest water level line of the cryopreservation tube 9 when it is shaken in the water bath, and the position of the lower plate 6 on the rod is the lowest water level line of the cryopreservation tube 9 when it is shaken in the water bath.

[0040] In a preferred embodiment, the position of the lower plate 6 on the rod is determined according to the cell liquid level in the cryopreservation tube 9 to be thawed. Specifically, the position of the lower plate 6 on the rod is higher than the cell liquid level in the cryopreservation tube 9 to be thawed, thereby ensuring that the cells to be thawed are always below the liquid level in the water bath.

[0041] The upper plate 4 is provided with a plurality of first test tube placement holes 41, and the lower plate 6 is provided with a plurality of second test tube placement holes 61. The distribution positions of the first test tube placement holes 41 on the upper plate 4 and the distribution positions of the second test tube placement holes 61 on the lower plate 6 are vertically aligned to allow the cryopreservation tubes 9 to pass through vertically. In this embodiment, there are five first test tube placement holes 41 and five second test tube placement holes 61 of the same size. The first test tube placement holes 41 and the second test tube placement holes 61 are arranged in a ring on their respective plates. The outer edge of the bottom plate 8 covers the outermost edge of all the second test tube placement holes 61 to support the bottom of the cryopreservation tubes 9.

[0042] In this embodiment, the main body of the support handle 1 is rod-shaped, the limiting block 3, the water level adjusting block 5, and the pad block 7 are all cylinders, and the pressure plate 2, the upper plate 4, the lower plate 6, and the bottom plate 8 are all circular plates. The pressure plate 2 has a certain thickness and weight to press down the cryopreservation tubes 9 in the test tube rack, preventing the cryopreservation tubes 9 from floating in the water bath and ensuring that the cell liquid level in the cryopreservation tubes 9 is always below the water level in the water bath. The radius of the bottom plate 8 is not less than the distance from the outermost point of each test tube placement hole to the center of the lower plate 6, thereby ensuring that the bottom plate 8 can block all test tube placement holes.

[0043] All components are made of rust-resistant metal or hard non-metallic materials. In this embodiment, the support handle 1, pressure plate 2, limiting block 3, upper plate 4, water level adjusting block 5, lower plate 6, pad block 7 and base plate 8 are all made of stainless steel.

[0044] Example 2

[0045] This embodiment provides a method for using a test tube rack for reviving cryopreserved tubes according to Embodiment 1, including the following steps:

[0046] S1. Select the appropriate test tube rack components and complete the assembly: In actual operation, ensure that the assembly of the test tube rack precedes the placement and removal of the cryopreservation tubes 9.

[0047] S1-1. Determine the length of the pad 7 based on the different height dimensions of the cryopreservation tubes 9. Determine the length of the water level regulating block 5 based on the water level range to be prepared in the water bath when the cryopreservation tubes 9 are shaken for thawing. At the same time, select the upper plate 4 and the lower plate 6 according to the number of cryopreservation tubes 9 to be thawed simultaneously, so that the number of first test tube placement holes 41 on the upper plate 4 and the number of second test tube placement holes 61 on the lower plate 6 are both no less than the number of cryopreservation tubes 9 to be thawed simultaneously, and the positions of the holes correspond one-to-one.

[0048] During the selection process of choosing the corresponding components of the test tube rack, an empty cryovial 9 without cells inside can be used as a reference for selecting each component, so as to reduce the adjustment time of the test tube rack when placing the cryovial 9.

[0049] S1-2. Using the support handle 1 as the rotation axis, insert and install the pressure plate 2, limit block 3, upper plate 4, water level adjustment block 5, lower plate 6, pad block 7, and base plate 8 sequentially from bottom to top on the threaded section of the support handle 1.

[0050] After rotating the first test tube placement hole 41 on the upper plate 4 and the second test tube placement hole 61 on the lower plate 6 to align them, tighten the limiting block 3, the water level adjusting block 5, the pad block 7, and the base plate 8 with threads.

[0051] During the process of aligning the first test tube placement hole 41 with the second test tube placement hole 61, an empty cryopreservation tube 9 without cells inside can also be used as a reference. Before the upper plate 4 and lower plate 6 are fixed, the alignment can be quickly completed by inserting the empty cryopreservation tube 9, and it can be removed after being fixed. This also ensures that no displacement or shift will occur during the assembly process.

[0052] S1-3, such as Figure 2 As shown, the test tube rack installation is complete.

[0053] S2. Place cryovials 9 on the test tube rack.

[0054] S2-1. When using this test tube rack, the pressure plate 2 is raised to the highest point of the support handle 1 by passing the protrusion 12 on the main body through the relief groove 22. Then, the pressure plate 2 is lowered after rotating a certain angle until it is placed on the protrusion 12. The pressure plate 2 can be temporarily fixed at the high point of the main body by relying on the protrusion 12 on the support handle 1.

[0055] S2-2. Insert the cryopreservation tubes 9 into the first test tube placement hole 41 on the upper plate 4 and the second test tube placement hole 61 on the lower plate 6, and place each cryopreservation tube 9 one by one, so that the bottom end of each cryopreservation tube 9 rests on the bottom plate 8.

[0056] S2-3. Rotate the pressure plate 2 until the relief groove 22 is aligned with the protrusion 12, and lower the pressure plate 2 until it presses down on the top of the cryopreservation tube 9, thereby ensuring that the cryopreservation tube 9 is limited and fixed in the height direction.

[0057] S3, Water bath thawing of cryopreservation tubes 9: (as shown in the image) Figure 3 As shown, immerse the test tube rack in the water bath. Note that the water level in the water bath should be between the upper plate 4 and the lower plate 6. Also, when shaking the test tube rack, ensure that the water level does not exceed the upper plate 4.

[0058] This utility model has a simple structure and can be designed with various matching test tube racks according to different cryopreservation tube sizes and the number of tubes to be thawed at the same time, so as to adapt to different usage environments.

[0059] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art can make various changes or equivalent substitutions to these features and embodiments without departing from the spirit and scope of the present invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are protected by the present invention.

Claims

1. A test tube rack for reviving cryopreserved tubes, characterized in that, Includes a support handle (1), the support handle (1) includes a body and a grip (11), the grip (11) is fixed to the top of the body, and the lower part of the body is provided with a threaded section; The main body is provided with a pressure plate (2), a limiting block (3), an upper plate (4), a water level regulating block (5), a lower plate (6), a pad block (7), and a bottom plate (8) that can move along the axial direction of the main body from top to bottom; Among them, the limiting block (3), water level adjusting block (5), pad block (7) and bottom plate (8) are all threaded to the threaded section, so that the upper plate (4) and the lower plate (6) are limited and fixed, and the pressure plate (2) can slide up and down along the axis of the body; The limiting block (3), water level adjusting block (5), pad block (7) and base plate (8) are all provided with threaded holes at their center positions; the pressure plate (2), upper plate (4) and lower plate (6) are all provided with center holes at their center positions; the upper plate (4) and lower plate (6) are also provided with a number of first test tube placement holes (41) and second test tube placement holes (61) for the cryopreservation tube (9) to pass through vertically, and the opening positions are one-to-one. The outer edge of the base plate (8) covers the outermost edge of all test tube placement holes to support the bottom end of the cryopreservation tube (9).

2. The test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The length of the pad (7) is determined according to the height of the cryopreservation tube (9) to be thawed.

3. A test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The length of the water level regulating block (5) is determined according to the water level range when the cryopreservation tube (9) is shaken during revival.

4. A test tube rack for reviving cryopreserved tubes according to claim 3, characterized in that, The position of the lower plate (6) on the rod is determined according to the cell liquid level in the cryopreservation tube (9) to be thawed.

5. A test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The threaded hole has an internal thread that matches the external thread of the threaded segment. The limiting block (3), water level adjusting block (5), pad block (7) and base plate (8) are threadedly fixed to the body through the threaded hole, so that the upper plate (4) located between the limiting block (3) and the water level adjusting block (5) and the lower plate (6) located between the water level adjusting block (5) and the pad block (7) are both limited and fixed to the body.

6. A test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The central hole is circular and its inner diameter is larger than the outer diameter of the body, so that the pressure plate (2), the upper plate (4), and the lower plate (6) can be fitted onto the body and slide up and down along the axial direction of the body through the central hole. The upper plate (4) is limited by the limiting block (3) and the water level adjusting block (5), and the lower plate (6) is limited by the water level adjusting block (5) and the pad block (7).

7. A test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The first test tube placement hole (41) is distributed in a ring shape on the upper plate (4), and the second test tube placement hole (61) is distributed in a ring shape on the lower plate (6).

8. A test tube rack for reviving cryopreserved tubes according to claim 1, characterized in that, The base plates (8) are all circular plates, and the radius of the base plate (8) is not less than the distance from the outermost point of each test tube placement hole to the center of the lower plate (6).

9. A test tube rack for reviving cryopreserved tubes according to any one of claims 1-8, characterized in that, The upper part of the body is provided with a protrusion (12), and a relief groove (22) is provided at the edge of the central hole on the pressure plate (2). The pressure plate (2) moves along the axial direction of the body through the relief groove (22) until it moves above the protrusion (12) and is temporarily fixed to the upper part of the body through the protrusion (12).

10. A test tube rack for reviving cryopreserved tubes according to claim 9, characterized in that, The protrusion (12) is located near the handle (11).

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