A vaccine production quantitative cell sub-packing pipetting device

Abstract

The utility model discloses a kind of vaccine production quantitative cell subpackaging pipetting equipment, including, base, base top is fixedly connected with support plate;Liquid storage tank is detachably in support plate top, liquid storage tank top is fixedly connected with liquid inlet pipe, liquid storage tank bottom is fixedly connected with liquid outlet pipe;Connecting pipe is detachably connected in liquid outlet pipe bottom, connecting pipe bottom is fixedly connected with multiple subpackaging pipes, and manual control valve is mounted on subpackaging pipe;Subpackaging jar is placed on base top, and subpackaging jar top is fixedly connected with connecting pipe;Quantitative adjusting pipe is set in subpackaging jar top, and quantitative adjusting pipe bottom extends into subpackaging jar, and its top is located outside subpackaging jar, and the air pressure in subpackaging jar can be discharged;Multiple subpackaging pipes are connected by connecting pipe, each subpackaging pipe corresponds independent subpackaging jar and is equipped with manual control valve, and multiple subpackaging jars can be simultaneously subpackaged, without completing single small jar process one by one, and subpackaging efficiency is significantly improved.

Description

Technical Field

[0001] This utility model relates to the field of cell suspension dispensing technology, and in particular to a quantitative cell dispensing and pipetting device for vaccine production. Background Technology

[0002] The semi-finished cell stage refers to cells that have not yet completed their final preparation / differentiation and require further processing or culture. Its core characteristic is that the cells have separated from their initial state, such as primary tissues or undifferentiated cells, but have not yet reached the standards for direct application, such as cell harvesting or finished product production; they are in an intermediate transitional stage. When cells are in the semi-finished stage, such as cells that have completed activation and expansion in cell culture but have not yet been formulated, they need to be dispensed for subsequent production and preservation. Currently, the dispensing of cell suspensions at this stage mainly involves extracting material from a master tank storing the cell suspension and dispensing it into target tanks of different capacities. Due to equipment limitations, the master tank cannot simultaneously and independently dispense quantitatively into multiple tanks of different capacities; the dispensing process must be completed one tank at a time, resulting in low operational efficiency. Utility Model Content

[0003] This application provides a quantitative cell dispensing and pipetting device for vaccine production. Multiple dispensing tubes are connected by a connecting tube, and each dispensing tube corresponds to an independent dispensing container and is equipped with a manual control valve. Dispensing operations can be performed on multiple dispensing containers simultaneously, eliminating the need to complete the process of each individual container one by one, thus significantly improving dispensing efficiency.

[0004] This application provides a quantitative cell dispensing and pipetting device for vaccine production, including:

[0005] The base has a support plate fixedly connected to its top, and the support plate is designed in an inverted L shape.

[0006] The liquid storage tank has two support bases fixedly mounted on its outer side. The support bases are fixed to the top of the support plate. The top of the liquid storage tank is fixedly connected to an inlet pipe, and the bottom of the liquid storage tank is fixedly connected to an outlet pipe. The outlet pipe passes through the support plate and extends to below its bottom.

[0007] A connecting pipe is detachably connected to the bottom of the outlet pipe. Multiple dispensing pipes are fixedly connected to the bottom of the connecting pipe, and manual control valves are installed on the dispensing pipes.

[0008] The dispensing tank is placed on top of the base. A connecting pipe is fixedly connected to the top of the dispensing tank. The connecting pipe and the connecting pipe are connected by a double threaded connecting pipe.

[0009] The metering regulating tube is located at the top of the dispensing tank, with its bottom extending into the tank and its top outside, allowing the gas pressure inside the tank to be released.

[0010] The metering tube is further equipped with a float that moves up and down inside the metering tube. The opening at the bottom of the metering tube is smaller than the outer diameter of the float. Two symmetrical blocks are fixedly connected to the inner wall of the metering tube, and the distance between the two blocks is smaller than the outer diameter of the float.

[0011] Furthermore, the opposing surfaces on the lower sides of the two stops are set as slopes.

[0012] The outer wall of the metering regulating tube is threaded, and the dispensing tank is threaded through a hole. The metering regulating tube is rotatably connected to the dispensing tank through the thread. A sleeve is fitted on the outside of the metering regulating tube. The bottom of the sleeve is fixedly connected to the top of the dispensing tank. The inner wall of the sleeve is threaded, and it is rotatably connected to the metering regulating tube.

[0013] The further dispensing containers are made of transparent medical-grade plastic, with graduation lines on the outer wall.

[0014] Furthermore, a placement slot is provided on the top of the base for placing the dispensing cans, with each dispensing can corresponding to a dispensing tube.

[0015] The inner wall of the placement groove is further provided with multiple limiting blocks. A medical-grade rubber sleeve is fixedly fitted on the outside of the limiting blocks. The limiting blocks are set as arc-shaped plates. A spring is fixedly connected to the side of the limiting block that is in contact with the placement groove.

[0016] One or more technical solutions provided in the embodiments of this application have at least the following technical effects or advantages: multiple dispensing pipes are connected by a connecting pipe, each dispensing pipe corresponds to an independent dispensing tank and is equipped with a manual control valve, which can simultaneously perform dispensing operations on multiple dispensing tanks without having to complete the process of each individual small tank, significantly improving dispensing efficiency; at the same time, the length of the quantitative adjustment tube inserted into the dispensing tank can be adjusted by turning the quantitative adjustment tube, and combined with the cooperation of the float and the stop block inside the quantitative adjustment tube, the float will automatically stop filling after the tube is blocked, which can independently preset and accurately control the dispensing capacity for different dispensing tanks, adapting to diverse quantitative needs. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the cell dispensing and pipetting device used in this application. Figure 1 ;

[0018] Figure 2 This is a schematic diagram of the cell dispensing and pipetting device used in this application. Figure 2 (Installation of double-threaded connecting pipe);

[0019] Figure 3 This is a front view of the cell dispensing and pipetting device of this application;

[0020] Figure 4 This is a schematic diagram of the sub-packaging tank structure for this application. Figure 1 ;

[0021] Figure 5This is a schematic diagram of the sub-packaging tank structure for this application. Figure 2 (Tighten the quantitative regulating tube);

[0022] Figure 6 Sectional view of the packaging tank for this application Figure 1 (The liquid is being filled and the float is in the reset state).

[0023] Figure 7 Sectional view of the packaging tank for this application Figure 2 (The liquid is being filled and the float is in a floating state).

[0024] Figure 8 This is a schematic diagram of the internal structure of the placement slot in this application.

[0025] In the diagram: 10 Base, 11 Placement slot, 12 Limiting block, 20 Support plate, 30 Storage tank, 31 Support base, 32 Inlet pipe, 33 Outlet pipe, 40 Connecting pipe, 41 Dispensing pipe, 42 Fixing ring, 43 Connecting pipe, 50 Dispensing tank, 51 Connecting pipe, 52 Sleeve, 53 Quantitative adjustment pipe, 54 Float, 55 Stop block, 60 Double threaded connecting pipe. Detailed Implementation

[0026] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. The drawings illustrate preferred embodiments of this utility model. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this utility model.

[0027] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] Example 1

[0030] Please see Figures 1-3A quantitative cell dispensing and pipetting device for vaccine production includes a base 10 and a support plate 20 fixedly connected to the base 10.

[0031] Specifically, the base 10 has support legs fixedly connected to the four corners at the bottom. The support plate 20 is set in an inverted L shape. The top of the support plate 20 is equipped with a liquid storage tank 30. Two support seats 31 are fixedly fitted on the outside of the liquid storage tank 30. The support seats 31 are fixed to the top of the support plate 20 with bolts, which facilitates the disassembly and assembly of the liquid storage tank 30 for cleaning. The top of the liquid storage tank 30 is fixedly connected to an inlet pipe 32 for adding cell suspension. The top of the inlet pipe 32 can be removably capped by a threaded connection. The bottom of the liquid storage tank 30 is fixedly connected to an outlet pipe 33. The outlet pipe 33 passes through the support plate 20 and extends to its bottom. The bottom of the outlet pipe 33 is detachably connected to a connecting pipe 40. The bottom of the connecting pipe 40 is fixedly connected to multiple dispensing pipes 41, which can dispense the cell suspension into individual tanks.

[0032] The bottom outer side of the outlet pipe 33 is fitted with a threaded connector, and the top of the connecting pipe 40 is pre-threaded. The fit between the threaded connector and the connecting pipe 40 facilitates the later disassembly and assembly of the connecting pipe 40. It should be noted that the inner diameter of the through hole at the top of the support plate 20 is 3-5mm larger than the outer diameter of the outlet pipe 33, so that the storage tank 30 can be removed from the support plate 20 for cleaning.

[0033] A fixing ring 42 is fixedly fitted on the outside of the connecting pipe 40. A support rod is fixedly connected to the side of the fixing ring 42 near the support plate 20. The support rod is fixed to the support plate 20 by bolts and is used for the installation support of the connecting pipe 40.

[0034] The dispensing tube 41 is equipped with a manual control valve, which can open each individual dispensing tube 41 individually. The bottom of the dispensing tube 41 is fixedly connected to a connecting tube 43.

[0035] Please see Figure 8 The base 10 has a placement slot 11 on its top, and a dispensing can 50 is placed in the placement slot 11. The dispensing can 50 corresponds one-to-one with the dispensing tube 41, that is, one dispensing tube 41 corresponds to one dispensing can 50.

[0036] Please see Figures 4-5 The top of the dispensing tank 50 is fixedly connected to a connecting pipe 51, which is connected to the connecting pipe 43 through a double-threaded connecting pipe 60. The cell suspension can be filled into the dispensing tank 50. The double-threaded connecting pipe 60 can be made of plastic corrugated pipe. The top of the dispensing tank 50 is also connected to a quantitative regulating pipe 53. The bottom of the quantitative regulating pipe 53 extends into the dispensing tank 50, and its top is located outside the dispensing tank 50, so that the air pressure inside the dispensing tank 50 can be discharged.

[0037] Please see Figures 6-7The metering tube 53 contains a float 54, which moves up and down within the tube. The bottom opening of the metering tube 53 is smaller than the outer diameter of the float 54 to prevent it from falling out. Two symmetrical baffles 55 are fixedly connected to the inner wall of the metering tube 53. The distance between the two baffles 55 is smaller than the outer diameter of the float. When the cell suspension is filled into the dispensing tank 50, as the liquid in the tank increases, some liquid will enter the tube from the bottom of the metering tube 53. The float 54 will then float up in the tube. When the float 54 floats up to the point where it is blocked between the two baffles 55, it will be unable to float up further and will seal the metering tube 53. This prevents the gas in the dispensing tank 50 from escaping, thus stopping the filling of the cell suspension (at this time, the manual control valve is in the open state).

[0038] The opposing surfaces on the lower sides of the two baffles 55 are set as slopes. During the upward movement of the float 54, the slopes on the lower sides of the baffles 55 can guide the float 54, preventing it from being difficult to accurately fit between the two baffles 55 due to deviation. This ensures that the float 54 can be stably positioned in the sealing position, reducing the sealing failure caused by the misalignment of the float 54. Moreover, the contact between the slope and the float 54 is a progressive fit. Compared with flat contact, this allows the float 54 and the baffles 55 to form a tighter fit, reducing gaps and lowering the risk of gas leakage from gaps causing liquid to continuously flow into the dispensing tank 50, further ensuring the accuracy of quantitative dispensing.

[0039] The outer wall of the quantitative adjustment tube 53 is threaded, and the dispensing tank 50 is threaded through the hole. The quantitative adjustment tube 53 is rotatably connected to the dispensing tank 50 through the thread. The length of the quantitative adjustment tube 53 in the dispensing tank 50 can be adjusted by screwing the quantitative adjustment tube 53, thereby controlling the volume of cell suspension filled in the dispensing tank 50.

[0040] The quantitative adjustment tube 53 is fitted with a sleeve 52 on the outside. The bottom of the sleeve 52 is fixedly connected to the top of the dispensing tank 50. The inner wall of the sleeve 52 is threaded, and it is rotatably connected to the quantitative adjustment tube 53. The direction of the thread inside the sleeve 52 is consistent with the direction of the thread of the threaded through hole on the dispensing tank 50 to ensure the normal rotation of the quantitative adjustment tube 53. It should be noted that after the dispensing tank 50 is filled, the double threaded connecting tube 60 connected to it should be removed, and then the quantitative adjustment tube 53 should be taken out. Plugs should be installed on the sleeve 52 and the connecting tube 51 respectively to prevent the cell suspension in the dispensing tank 50 from leaking out.

[0041] The dispensing container 50 can be made of transparent medical-grade plastic, with graduation lines on its outer wall. The distance that the float 54 floats up and down in the quantitative adjustment tube 53 can be defined as 5-10cm or can be defined by the manufacturer according to the requirements during production, so as to determine the capacity of the cell suspension filled in the dispensing container 50 later (there will be some error in the filling capacity, but it will not be too large and can be ignored).

[0042] Please see Figure 8 The inner wall of the placement groove 11 is provided with multiple limiting blocks 12. The outer side of the limiting block 12 is fixedly fitted with a medical-grade rubber sleeve. The limiting block 12 is set as an arc plate. A spring is fixedly connected to the side of the limiting block 12 that is in contact with the placement groove 11. The extension of the spring can make the limiting block 12 stick tightly to the outer wall of the dispensing container 50, further ensuring the stability of the dispensing container 50 when placed in the placement groove 11.

[0043] The 50-ton repackaging tank can be customized to different sizes to meet specific needs.

[0044] In actual operation of this embodiment, the dispensing tank 50 is placed in the base placement groove 11. The limiting block 12 in the groove is fixed to the outer wall of the dispensing tank 50 by the spring force, and the connecting pipe 51 of the dispensing tank 50 is connected to the connecting pipe 43 of the dispensing pipe 41 through the double threaded connecting pipe 60. According to the required dispensing capacity, the quantitative adjustment pipe 53 is turned to adjust its length extending into the dispensing tank 50 to complete the quantitative preset.

[0045] Open the manual control valve on the target dispensing tube 41. The cell suspension in the storage tank 30 flows sequentially into the corresponding dispensing tank 50 through the outlet tube 33, connecting tube 40, dispensing tube 41, connecting tube 43, and connecting tube 51. As the liquid in the tank increases, the liquid enters the metering regulating tube 53, pushing the float 54 in the tube to rise until the float 54 is blocked by the two baffles 55 in the tube. At this time, the metering regulating tube 53 is sealed, and the gas in the dispensing tank 50 cannot be discharged, so the liquid filling automatically stops.

[0046] Close the manual control valve, remove the double-threaded connecting pipe 60 between the dispensing tank 50 and the dispensing pipe 41, remove the quantitative adjustment pipe 53, install plugs on the sleeve 52 and connecting pipe 51 of the dispensing tank 50 to prevent leakage, and finally remove the dispensing tank 50 after dispensing.

[0047] The above description is merely a preferred embodiment of this utility model and is not intended to limit the scope of this utility model. For those skilled in the art, various modifications and variations are possible with this utility model. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A quantitative cell dispensing and pipetting device for vaccine production, characterized in that: include, The base has a support plate fixedly connected to its top, and the support plate is designed in an inverted L shape. The liquid storage tank has two support bases fixedly mounted on its outer side. The support bases are fixed to the top of the support plate. The top of the liquid storage tank is fixedly connected to an inlet pipe, and the bottom of the liquid storage tank is fixedly connected to an outlet pipe. The outlet pipe passes through the support plate and extends to below its bottom. A connecting pipe is detachably connected to the bottom of the outlet pipe. Multiple dispensing pipes are fixedly connected to the bottom of the connecting pipe, and manual control valves are installed on the dispensing pipes. The dispensing tank is placed on top of the base. A connecting pipe is fixedly connected to the top of the dispensing tank. The connecting pipe and the connecting pipe are connected by a double threaded connecting pipe. The metering regulating tube is located at the top of the dispensing tank, with its bottom extending into the tank and its top outside, allowing the gas pressure inside the tank to be released.

2. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 1, characterized in that: The quantitative regulating tube contains a float that moves up and down within the tube. The opening at the bottom of the tube is smaller than the outer diameter of the float. Two symmetrical blocks are fixedly connected to the inner wall of the tube, and the distance between the two blocks is smaller than the outer diameter of the float.

3. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 2, characterized in that: The opposing surfaces on the lower sides of the two blocks are set as inclined planes.

4. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 1, characterized in that: The quantitative regulating tube has threads on its outer wall and a threaded through hole on the dispensing tank. The quantitative regulating tube is rotatably connected to the dispensing tank through the threads. A sleeve is fitted on the outside of the quantitative regulating tube. The bottom of the sleeve is fixedly connected to the top of the dispensing tank. The inner wall of the sleeve has threads, which are rotatably connected to the quantitative regulating tube.

5. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 1, characterized in that: The dispensing container is a transparent medical-grade plastic container with graduation lines on its outer wall.

6. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 1, characterized in that: The base has a slot on top for placing the dispensing cans, with each dispensing can corresponding to a dispensing tube.

7. The quantitative cell dispensing and pipetting device for vaccine production as described in claim 6, characterized in that: The inner wall of the placement groove is provided with multiple limiting blocks. A medical-grade rubber sleeve is fixedly fitted on the outside of the limiting blocks. The limiting blocks are set as arc-shaped plates. A spring is fixedly connected to the side of the limiting block that is in contact with the placement groove.

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