Transparent viewing window embedded PCR cover

Abstract

The utility model provides a transparent observation window embedded type PCR cover body for covering the working cavity opening of PCR amplifier body, include: cover body, cover body is hollow rectangular structure, top is provided with the stepped observation window installation groove, bottom edge is provided with annular sealing groove, embedded type observation window subassembly, embedded type observation window subassembly includes the observation window main part made of high -transparency borosilicate glass, the edge of observation window main part is processed with annular boss, annular boss is adapted to install in observation window installation groove, sealing structure, sealing structure includes the silicone rubber sealing washer of wrapping in the outside of annular boss, fills in the high temperature resistance sealant of observation window installation groove and annular boss between and the foamed silicone rubber sealing strip of embedding in annular sealing groove, anti -fog system, anti -fog system includes the annular heating wire of fixed mounting in the inside of cover body, annular heating wire outside is equipped with the insulating ceramic sleeve, and annular heating wire electric connection has temperature sensor.

Description

Technical Field

[0001] This utility model relates to the field of PCR amplification instruments, and in particular to a transparent observation window embedded PCR cover. Background Technology

[0002] PCR (polymerase chain reaction) amplification instruments are widely used in the field of molecular biology. They are mainly used to realize the in vitro amplification of DNA and play a vital role in many fields such as gene detection, disease diagnosis, and biopharmaceuticals. By simulating the DNA replication process in organisms, PCR uses DNA polymerase to replicate specific DNA fragments in large quantities under specific in vitro conditions, thereby meeting the needs of subsequent research and detection.

[0003] During PCR amplification, real-time observation of the sample's reaction is crucial for the success of the experiment. Traditional PCR amplification instruments are usually equipped with a top-cover observation window. However, these existing observation windows have many technical defects, which seriously affect the reliability and operability of PCR amplification experiments.

[0004] The sealing structure of the observation window on the top cover of traditional PCR amplification instruments is not sufficiently robust, making it difficult to achieve an effective seal. This allows external air, dust, microorganisms, and other impurities to easily enter the working chamber of the PCR amplification instrument, contaminating the samples. Once the samples are contaminated, it not only affects the accuracy of PCR amplification results, leading to experimental data deviations, but may even cause the entire experiment to fail, resulting in a waste of time and resources. During PCR amplification, high temperatures and moisture are generated inside the working chamber. Because traditional observation windows lack effective anti-fogging measures, moisture easily condenses on the surface of the observation window, making it blurry. This significantly affects the operator's real-time observation of the sample reaction, making it impossible to accurately and promptly grasp the experimental progress and hindering effective monitoring and adjustment of the experimental process. Utility Model Content

[0005] To address the problem of poor sealing and fogging of the observation window on the top cover of traditional PCR amplification instruments in the prior art, this utility model provides a transparent observation window embedded PCR cover.

[0006] The transparent observation window embedded PCR cover provided by this utility model adopts the following technical solution:

[0007] A transparent observation window embedded PCR cover is used to cover the working cavity opening of the PCR amplification instrument body, comprising: a cover body, wherein the cover body is a hollow rectangular structure, a stepped observation window mounting groove is provided at the top, and an annular sealing groove is provided at the bottom edge;

[0008] An embedded observation window assembly includes an observation window body made of high-transparency borosilicate glass, the edge of which is machined with an annular boss, which is adapted to be installed in the observation window mounting groove.

[0009] The sealing structure includes a silicone rubber sealing ring wrapped around the outside of the annular boss, a high-temperature resistant sealant filling the space between the observation window mounting groove and the annular boss, and a foamed silicone rubber sealing strip embedded in the annular sealing groove.

[0010] An anti-fog system, comprising an annular heating wire fixedly installed inside the cover body; an insulating ceramic sleeve is fitted over the annular heating wire, and a temperature sensor is electrically connected to the annular heating wire;

[0011] A quick-fixing mechanism includes elastic buckles disposed on the left and right sides of the cover body, and slots corresponding to the edges of the working chamber openings of the PCR amplification instrument body, wherein the elastic buckles and slots are adapted to engage.

[0012] Furthermore, the surface of the main body of the observation window is coated with a nano anti-fog coating, which is a SiO2-TiO2 composite coating with a thickness of 50-100nm;

[0013] Furthermore, the embedded observation window assembly also includes a stainless steel pressure ring, which is fixed to the top of the observation window mounting groove of the cover body by bolts, and a fluororubber gasket is provided between the stainless steel pressure ring and the observation window body.

[0014] Furthermore, the annular heating wire is made of nickel-chromium alloy and has a power of 5-8W; the temperature sensor is an NTC thermistor, and its detection end is close to the inner surface of the observation window body.

[0015] Furthermore, the elastic buckle is made of polypropylene, and at least one elastic buckle is provided on each side of each cover body. The end of the elastic buckle is processed with an inclined guide surface with an inclination angle of 30-45°.

[0016] Furthermore, the top surface of the cover body is provided with anti-slip texture, which is a cross-grid pattern with a depth of 0.5-1mm.

[0017] In summary, the beneficial effects of this utility model are as follows:

[0018] This invention improves upon existing cover plates to achieve embedded sealing and fixation of the observation window, effectively enhancing sealing performance and preventing sample contamination. An anti-fogging system is incorporated to promptly eliminate fog on the observation window surface, ensuring clear real-time observation. A quick-fixing mechanism ensures a more secure cover fixation, preventing detachment. Furthermore, the rational design mitigates the limitation of observation angles, thereby enhancing the reliability and operability of the PCR amplification process. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the present invention installed on the main body of a PCR amplification instrument;

[0020] Figure 2 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the overall internal structure of this utility model;

[0022] Figure 4 This utility model Figure 3 An enlarged schematic diagram of part A in the middle;

[0023] Figure 5 This is a schematic diagram of the overall structure of this utility model without the stainless steel pressure ring;

[0024] Figure 6 This utility model Figure 5 Enlarged schematic diagram of part B in the middle;

[0025] Figure 7 This is a schematic diagram of the overall structure of the anti-fog system of this utility model.

[0026] As shown in the figure: 0-PCR amplification instrument body, 1-cover body, 11-observation window mounting slot, 12-annular sealing groove, 2-embedded observation window assembly, 21-observation window body, 22-annular boss, 23-nano anti-fog coating, 24-stainless steel pressure ring, 25-bolt, 3-sealing structure, 31-silicone rubber sealing ring, 32-high temperature resistant sealant, 33-foamed silicone rubber sealing strip, 4-anti-fog system, 41-annular heating wire, 43-temperature sensor, 5-quick fixing mechanism, 51-elastic buckle, 511-tilted guide surface, 52-slot. Detailed Implementation

[0027] The following is in conjunction with the appendix Figure 1-7 The present invention will be further described in detail below:

[0028] This utility model discloses a transparent observation window embedded PCR cover, such as... Figure 1-7As shown, a transparent observation window embedded PCR cover is used to cover the working chamber opening of the PCR amplification instrument body 0. It includes: a cover body 1, which is a hollow rectangular structure with a stepped observation window mounting groove 11 at the top and an annular sealing groove 12 at the bottom edge; an embedded observation window assembly 2, which includes an observation window body 21 made of high-transparency borosilicate glass, with an annular boss 22 machined on the edge of the observation window body 21, the annular boss 22 being fitted into the observation window mounting groove 11; and a sealing structure 3, which includes a silicone rubber sealing ring 31 wrapped around the annular boss 22, and a filler... High-temperature resistant sealant 32 fills the space between the observation window mounting groove 11 and the annular boss 22, and foamed silicone rubber sealing strip 33 is embedded in the annular sealing groove 12; anti-fog system 4, the anti-fog system 4 includes an annular heating wire 41 fixedly installed on the inner side of the cover body 1; an insulating ceramic sleeve is sleeved on the outer side of the annular heating wire 41, and the annular heating wire 41 is electrically connected to a temperature sensor 43; quick fixing mechanism 5, the quick fixing mechanism 5 includes elastic buckles 51 set on the left and right sides of the cover body 1, and slots 52 corresponding to the edge of the working chamber opening of the PCR amplification instrument body 0, the elastic buckles 51 and the slots 52 are adapted to engage;

[0029] In the above embodiments, the cover body 1 is designed as a hollow rectangular structure, which is adapted to the opening of the working chamber of the PCR amplification instrument body 0 and can effectively cover the working chamber; the stepped observation window mounting slot 11 at the top provides an installation position for the embedded observation window assembly 2, and the annular sealing groove 12 at the bottom edge is used to install the sealing structure 3 to ensure the sealing between the cover and the working chamber; the hollow rectangular structure reduces the overall weight while ensuring the strength of the cover; the stepped observation window mounting slot 11 facilitates the positioning and installation of the observation window assembly 2, and the annular sealing groove 12 provides a basis for subsequent sealing measures, which helps to prevent sample contamination and improve the reliability of the PCR amplification process;

[0030] The main body of the observation window 21 is made of high-transparency borosilicate glass, which has good optical performance and can clearly observe the PCR amplification process. The annular protrusion 22 processed on the edge is adapted to the observation window mounting groove 11. Through this embedded design, the main body of the observation window 21 can be stably installed on the cover body 1. The high-transparency borosilicate glass ensures the clarity of observation, and the embedded installation method improves the stability and sealing of the observation window 21, reduces the interference of external factors on observation, and also avoids the observation window 21 from becoming loose or shifting during use.

[0031] A silicone rubber sealing ring 31 wraps around the outside of the annular boss 22, utilizing the elasticity and sealing properties of silicone rubber to fill the gap between the annular boss 22 and the observation window mounting groove 11, preventing the entry of external air and impurities. High-temperature resistant sealant 32 fills the space between the observation window mounting groove 11 and the annular boss 22, further enhancing the sealing effect and enabling it to withstand the high-temperature environment during PCR amplification. A foamed silicone rubber sealing strip 33 is embedded in the annular sealing groove 12, acting as a buffer and seal when the cover contacts the working chamber opening of the PCR amplification instrument body 0, preventing sample leakage and external contamination. The design of the multiple sealing structure 3 greatly improves the sealing performance of the cover, effectively preventing sample contamination and ensuring the accuracy and reliability of the PCR amplification experiment. Simultaneously, the use of high-temperature resistant sealant 32 and silicone rubber ensures the stability and durability of the sealing structure 3 under high-temperature conditions. The annular heating wire 41 is fixedly installed inside the cover body 1. When energized, it generates heat, which raises the surface temperature of the observation window body 21 through heat transfer, thereby preventing water vapor from condensing into fog on the surface of the observation window body 21. An insulating ceramic sleeve is fitted on the outside of the annular heating wire 41, which serves to insulate and protect against leakage accidents. The temperature sensor 43 is electrically connected to the annular heating wire 41, which monitors the temperature of the observation window body 21 in real time and automatically adjusts the power of the annular heating wire 41 according to the set temperature value to achieve precise temperature control. The anti-fogging system 4 effectively solves the problem of fogging in the observation window 21, ensuring that the changes in the sample can be observed in real time and clearly during PCR amplification. The use of the temperature sensor 43 achieves precise temperature control, avoiding the impact of excessively high or low temperatures on the observation window 21 and the sample, while also improving energy utilization efficiency.

[0032] like Figure 1-7 As shown, the surface of the observation window body 21 is coated with a nano-anti-fog coating 23. The nano-anti-fog coating 23 is a SiO2-TiO2 composite coating with a thickness of 50-100 nm. In this embodiment, the nano-anti-fog coating 23 is a SiO2-TiO2 composite coating with a special microstructure and chemical properties. SiO2 has good hydrophilicity, which allows water vapor to spread rapidly on the surface of the coating 23 to form a uniform water film instead of condensing into water droplets, thereby achieving an anti-fog effect. TiO2 has photocatalytic properties, which can decompose organic pollutants and keep the surface of the coating 23 clean. The nano-anti-fog coating 23 further enhances the anti-fog performance of the observation window 21, making observation clearer. At the same time, its photocatalytic properties help keep the surface of the observation window body 21 clean and extend the service life of the observation window 21.

[0033] like Figure 1-7As shown, the embedded observation window assembly 2 also includes a stainless steel pressure ring 24, which is fixed to the top of the observation window mounting groove 11 of the cover body 1 by bolts 25. A fluororubber gasket is provided between the stainless steel pressure ring 24 and the observation window body 21. In this embodiment, the stainless steel pressure ring 24 is fixed to the top of the observation window mounting groove 11 of the cover body 1 by bolts 25, which further fixes the observation window body 21. The fluororubber gasket is placed between the stainless steel pressure ring 24 and the observation window body 21. By utilizing the elasticity and sealing performance of fluororubber, it fills the gap between the two, enhances the sealing effect, and at the same time avoids direct contact between the stainless steel pressure ring 24 and the observation window body 21, thus preventing damage to the observation window body 21.

[0034] The annular heating wire 41 is made of nickel-chromium alloy and has a power of 5-8W. The temperature sensor 43 is an NTC thermistor, and its detection end is close to the inner surface of the observation window body 21. In this embodiment, the annular heating wire 41 is made of nickel-chromium alloy, which has good high temperature resistance and resistance stability, and can work stably for a long time in high temperature environment. With a power of 5-8W, it can provide enough heat to raise the surface temperature of the observation window body 21 to achieve the anti-fogging effect, while not consuming too much energy.

[0035] The elastic buckle 51 is made of polypropylene. At least one elastic buckle 51 is provided on each side of each cover body 1. The end of the elastic buckle 51 is machined with an inclined guide surface 511 with an inclination angle of 30-45°. In this embodiment, the elastic buckle 51 is made of polypropylene, which has good elasticity and toughness and can recover its original shape after multiple deformations, thus ensuring the service life of the elastic buckle 51. The inclined guide surface 511 has an inclination angle of 30-45°. When installing the cover, the inclined guide surface 511 can guide the elastic buckle 51 to smoothly snap into the slot 52, reducing the installation difficulty.

[0036] The top surface of the cover body 1 is provided with anti-slip texture. The anti-slip texture is a cross-grid pattern with a depth of 0.5-1mm. In this embodiment, the cross-grid anti-slip texture on the top surface of the cover body 1 increases the surface friction. When the operator holds the cover to install or remove it, the anti-slip texture can prevent the hand from sliding on the surface of the cover, thus improving the safety and stability of the operation.

[0037] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. The various components mentioned in this utility model are common technologies in the existing field. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and description described above...

[0038] The description is merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A transparent observation window embedded PCR cover for covering the working chamber opening of a PCR amplification instrument body (0), characterized in that, include: The cover body (1) is a hollow rectangular structure with a stepped observation window mounting groove (11) on the top and an annular sealing groove (12) on the bottom edge. An embedded observation window assembly (2) includes an observation window body (21) made of high-transparency borosilicate glass, the edge of which is machined with an annular boss (22), the annular boss (22) being adapted to be installed in the observation window mounting groove (11); The sealing structure (3) includes a silicone rubber sealing ring (31) wrapped around the outside of the annular boss (22), a high-temperature resistant sealant (32) filled between the observation window mounting groove (11) and the annular boss (22), and a foamed silicone rubber sealing strip (33) embedded in the annular sealing groove (12). The anti-fog system (4) includes an annular heating wire (41) fixedly installed inside the cover body (1); an insulating ceramic sleeve is provided on the outside of the annular heating wire (41); and a temperature sensor (43) is electrically connected to the annular heating wire (41). The quick-fixing mechanism (5) includes elastic buckles (51) disposed on the left and right sides of the cover body (1) and slots (52) corresponding to the edge of the working chamber opening of the PCR amplification instrument body. The elastic buckles (51) and slots (52) are adapted to engage.

2. The transparent observation window embedded PCR cover according to claim 1, characterized in that, The surface of the observation window body (21) is coated with a nano anti-fog coating (23), which is a SiO2-TiO2 composite coating with a thickness of 50-100nm.

3. The transparent observation window embedded PCR cover according to claim 1, characterized in that, The embedded observation window assembly (2) also includes a stainless steel pressure ring (24), which is fixed to the top of the observation window mounting groove (11) of the cover body (1) by bolts (25), and a fluororubber gasket is provided between the stainless steel pressure ring (24) and the observation window body (21).

4. The transparent observation window embedded PCR cover according to claim 1, characterized in that, The annular heating wire (41) is made of nickel-chromium alloy and has a power of 5-8W. The temperature sensor (43) is an NTC thermistor, and its detection end is close to the inner surface of the observation window body (21).

5. The transparent observation window embedded PCR cover according to claim 1, characterized in that, The elastic buckle (51) is made of polypropylene. Each of the cover body (1) has at least one elastic buckle (51) on each side. The end of the elastic buckle (51) is processed with an inclined guide surface (511) with an inclination angle of 30-45°.

6. The transparent observation window embedded PCR cover according to claim 1, characterized in that, The top surface of the cover body (1) is provided with anti-slip texture, which is a cross-grid pattern with a depth of 0.5-1mm.

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