Sample pretreatment module and constant temperature nucleic acid amplification detection device

Abstract

The utility model provides a kind of sample pretreatment module and thermostatic nucleic acid amplification detection equipment, sample pretreatment module includes carousel assembly, jacking assembly, mixing assembly and ultrasonic assembly, carousel assembly is provided with outer circle cup site, inner circle cup site and first carousel, outer circle cup site includes sequentially arranged carry one, mixing site one, incubation site and exit one, inner circle cup site includes sequentially arranged carry two, mixing site two, ultrasonic site and exit two, first carousel is set with multiple and carry one, mixing site one, incubation site, exit one, carry two, mixing site two, ultrasonic site and exit two corresponding setting placement hole of ultrasonic site;Mixing assembly includes two mixing mechanisms respectively arranged below mixing site one and mixing site two;Ultrasonic assembly includes ultrasonic head arranged below ultrasonic site, jacking assembly drives ultrasonic head and two mixing mechanisms to move up and down;Thermostatic nucleic acid amplification detection equipment further includes sample pretreatment module;The utility model can take into account different sample test requirements.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, specifically to a sample preprocessing module and an isothermal nucleic acid amplification and detection device. Background Technology

[0002] In in vitro diagnostic equipment, sample pretreatment methods include physical processing, chemical processing, biological processing, and inactivation treatment. Physical processing includes methods such as shaking and mixing, ultrasonic lysis, and centrifugation. The method of sample pretreatment varies depending on the test purpose. In existing technologies, the sample pretreatment module performs the function of processing a single sample and cannot be compatible with sample pretreatment functions for multiple test purposes, resulting in low applicability. When integrated with other modules, the detection efficiency is low.

[0003] Existing isothermal nucleic acid amplification instruments not only have small sample volumes, but also pose a risk of contamination due to manual operation. Especially during disease outbreaks, their testing cycles are too long for large batches of samples, failing to meet testing needs. Utility Model Content

[0004] The primary objective of this invention is to provide a sample preprocessing module that can accommodate different sample testing needs.

[0005] The second objective of this invention is to provide an isothermal nucleic acid amplification and detection device that includes the above-mentioned sample pretreatment module.

[0006] To achieve the aforementioned first objective, this utility model provides a sample preprocessing module, including a turntable assembly, a rotation drive assembly, a lifting assembly, and a mixing assembly. The turntable assembly is provided with an outer ring cup placement position, an inner ring cup placement position, and a first turntable. The rotation drive assembly drives the first turntable to rotate around its axis. The mixing assembly includes a mixing drive device and two mixing mechanisms. The mixing drive device drives the mixing mechanisms to rotate. The lifting assembly drives the two mixing mechanisms to move up and down. The outer ring cup placement position includes a sequentially arranged infeed position 1, a mixing position 1, an incubation position 1, and an outfeed position 1. The inner ring cup placement... The sample pretreatment module includes a second intake position, a second mixing position, an ultrasonic position, and a second output position, arranged sequentially. The first turntable has multiple placement holes, which correspond one-to-one with the first intake position, the first mixing position, the incubation position, the first output position, the second intake position, the second mixing position, the ultrasonic position, and the second output position. Two mixing mechanisms are respectively located below the first mixing position and the second mixing position. The sample pretreatment module also includes an ultrasonic component, which includes an ultrasonic controller and an ultrasonic head. The ultrasonic head is located below the ultrasonic position. The ultrasonic controller controls the ultrasonic head to emit ultrasonic waves, and the lifting component drives the ultrasonic head to move up and down.

[0007] As can be seen from the above scheme, with the above settings, samples placed in the outer ring cup position and the inner ring cup position can be independently subjected to different sample pretreatments. The outer ring cup position is used to place the first sample, which can realize oscillation mixing and incubation pretreatment, while the inner ring cup position is used to place the second sample, which can realize oscillation mixing and ultrasonic pretreatment. This utility model can be compatible with two different sample testing needs and has the advantages of simple structure, small size and flexible application to different scenarios.

[0008] A further embodiment is that the turntable assembly includes a housing, a top cover, and at least three pressure cup sleeves. The first turntable is rotatably disposed inside the housing. The top cover is fitted onto the upper part of the housing. The top cover has at least four through holes, which are respectively configured and connected to and connected to the first inlet, the first outlet, the second inlet, and the second outlet. Pressure cup sleeves are disposed on the top cover. Pressure cup sleeves are disposed above the first mixing position, the second mixing position, and the ultrasonic position.

[0009] As can be seen from the above scheme, the above settings facilitate the insertion of samples from carry-in one or carry-in two, and the samples are sequentially transferred between each working position under the rotation of the first turntable, and then taken out from exit-out one or exit-out two after preprocessing.

[0010] A further proposed approach is to set the number of incubation positions to two or more, with all incubation positions arranged adjacent to each other; the number of ultrasound positions is set to two or more, with all ultrasound positions arranged adjacent to each other; the number of ultrasound heads is equal to the number of ultrasound positions, and ultrasound heads and ultrasound positions are set in a one-to-one correspondence.

[0011] As can be seen from the above scheme, the above settings help to increase the number of incubation sites and ultrasound sites, thus ensuring the accuracy of the test results.

[0012] A further embodiment is that an electric heating film and a first insulation layer are provided on the bottom wall and / or side wall of the shell, with the first insulation layer located on the outward side of the electric heating film; and a second insulation layer is provided on the outer side and / or inner side of the top cover.

[0013] As can be seen from the above scheme, the above settings facilitate the pretreatment of samples in a constant temperature environment.

[0014] A further embodiment is that the lifting assembly includes a lifting drive device and a lifting plate. The lifting plate is located below the housing, and the mixing mechanism and the ultrasonic head are both located on the lifting plate. The lifting drive device drives the mixing mechanism and the ultrasonic head to move up and down simultaneously through the lifting plate, so that the upper part of the mixing mechanism and the upper part of the ultrasonic head extend into the corresponding mixing position and ultrasonic position, respectively.

[0015] As can be seen from the above scheme, the above settings facilitate the connection and separation of the sample tube from the mixing mechanism and the ultrasonic head.

[0016] A further embodiment is that the sample preprocessing module also includes a clamping assembly, which is set on the lifting plate and moves up and down with the lifting plate. The clamping assembly has two clamping ports, which are respectively located below the first and second output positions. The clamping assembly includes a clamping drive device, a gripper one and a gripper two. The gripper one and the gripper two are arranged opposite each other and form two clamping ports. The clamping drive device drives the gripper one and / or the gripper two to move closer or further apart.

[0017] As can be seen from the above scheme, with the above settings, after the pretreatment is completed, the clamping component clamps the two sample tubes in outlet one and outlet two at the same time, which facilitates subsequent capping and liquid aspiration.

[0018] A further embodiment is that the mixing mechanism includes a telescopic coupling, an eccentric mixing shaft, and a mixing seat. The telescopic coupling is movably inserted into a lifting plate, which can drive the telescopic coupling to extend and retract. The eccentric mixing shaft is located on the upper part of the telescopic coupling, and the mixing seat is located on the upper part of the eccentric mixing shaft. The mixing drive device drives the telescopic coupling to rotate around its axis, which in turn drives the mixing seat to rotate eccentrically through the eccentric mixing shaft.

[0019] As can be seen from the above scheme, the above settings can realize the up and down movement of the mixing seat, which is convenient for connection with the lower part of the sample tube, and can also realize the eccentric rotation of the mixing seat, which is convenient for oscillating and mixing the sample tube with the help of the pressure cup sleeve.

[0020] To achieve the second objective mentioned above, this utility model provides an isothermal nucleic acid amplification and detection device, which includes the sample preprocessing module described above.

[0021] A further embodiment of the isothermal nucleic acid amplification and detection equipment includes a sample module, a pipette tip module, a reagent module, an auxiliary reagent module, a mixing module, a centrifugation module, a waste bin, and an incubation and interpretation module. The sample module is located on one side of the sample pretreatment module in the Y direction, the incubation and interpretation module is located on one side of the sample pretreatment module in the X direction, the pipette tip module is located on one side of the incubation and interpretation module in the Y direction, and is also located on one side of the sample module. The reagent module is located between the incubation and interpretation module and the pipette tip module. The auxiliary reagent module, the mixing module, the centrifugation module, and the waste bin are all located between the incubation and interpretation module and the reagent module, with the mixing module located between the auxiliary reagent module and the centrifugation module, and the waste bin located between the auxiliary reagent module and the incubation and interpretation module.

[0022] As can be seen from the above scheme, the above setup is conducive to the integration and rational layout of various modules in the isothermal nucleic acid amplification and detection equipment, which helps to reduce its size and floor space, increase its throughput, reduce detection time and improve measurement efficiency.

[0023] A further embodiment of the isothermal nucleic acid amplification and detection device includes a capping gripper, a puncture and capping gripper, a pipetting arm, a first reagent transfer arm, and a second reagent transfer arm. The capping gripper moves back and forth between the sample module and the sample pretreatment module; the puncture and capping gripper moves back and forth between the reagent module and the mixing module; the pipetting arm moves back and forth between the pipette tip module, the auxiliary reagent module, the sample pretreatment module, the mixing module, and the waste bin; the first reagent transfer arm moves back and forth between the mixing module, the centrifugation module, and the incubation and interpretation module; and the second reagent transfer arm moves back and forth between the incubation and interpretation module, the mixing module, and the waste bin.

[0024] As can be seen from the above scheme, with the above settings, each gripper and transfer arm performs its own function and executes the corresponding actions in an orderly manner according to the detection steps, which helps to save time and improve detection efficiency. Attached Figure Description

[0025] Figure 1 This is a structural diagram from the first perspective of an embodiment of the sample preprocessing module of this utility model.

[0026] Figure 2 This is a structural diagram from a second perspective of an embodiment of the sample preprocessing module of this utility model.

[0027] Figure 3 This is a cross-sectional view from the third perspective of an embodiment of the sample preprocessing module of this utility model.

[0028] Figure 4 This is a cross-sectional view from the fourth perspective of an embodiment of the sample preprocessing module of this utility model.

[0029] Figure 5 This is a cross-sectional view from the fifth perspective of an embodiment of the sample preprocessing module of this utility model.

[0030] Figure 6 This is a structural diagram of the clamping component in an embodiment of the sample preprocessing module of this utility model.

[0031] Figure 7 This is a cross-sectional view of the clamping component in an embodiment of the sample preprocessing module of this utility model.

[0032] Figure 8 This is a structural diagram of an embodiment of the isothermal nucleic acid amplification and detection device of this utility model.

[0033] Explanation of reference numerals in the attached figures: 1-Turntable assembly, 11-Shell, 12-Top cover, 13-First turntable, 131-Placement hole, 14-Cup sleeve, 15-Outer ring cup placement position, 151-Entry position one, 152-Mixing position one, 153-Incubation position, 154-Exit position one, 16-Inner ring cup placement position, 161-Entry position two, 162-Mixing position two, 163-Ultrasonic position, 164-Exit position two; 2-Rotary drive assembly, 21-Rotary motor, 22-Main shaft, 23-First driving pulley, 24-First synchronous belt, 25-First driven pulley; 3-Lifting assembly, 31-Lifting motor, 32-First lead screw nut, 33-Lifting plate; 4-Mixing assembly, 41-Mixing drive device, 411-Mixing motor, 412-Second driving wheel, 413-Second synchronous belt, 414-Second driven wheel, 42-Mixing mechanism, 421-convex coupling, 422-concave coupling, 423-Eccentric mixing shaft, 424-Mixing seat; 5-Ultrasound assembly, 51-Ultrasound controller, 52-Ultrasound head; 6-Clamping assembly, 61-Fixed plate, 62-Clamping drive device, 621-Clamping motor, 622-Modible seat, 623-Second lead screw nut, 624-Guide rail, 63-Gripper one, 64-Gripper two, 65-Clamping port, 66-Buffer pad; 7-Sample tubes; 10-Sample pretreatment module, 20-Sample module, 30-Capping gripper, 40-Pipette tip module, 50-Reagent module, 60-Auxiliary reagent module, 70-Mixing module, 80-Centrifugation module, 90-Trash can, 100-Incubation interpretation module, 110-Puncture capping gripper, 120-Pipette arm, 130-First reagent transfer arm, 140-Second reagent transfer arm, 150-Reaction tube.

[0034] The present invention will be further described below with reference to the accompanying drawings and embodiments. Detailed Implementation

[0035] Example of a sample preprocessing module: See Figures 1 to 4 The sample preprocessing module 10 provided in this embodiment includes a turntable assembly 1, a rotation drive assembly 2, a lifting assembly 3, a mixing assembly 4, and an ultrasound assembly 5.

[0036] The turntable assembly 1 includes a housing 11, a top cover 12, a first turntable 13, and at least three pressure cup sleeves 14. The first turntable 13 is rotatably disposed inside the housing 11, and the top cover 12 covers the upper opening of the housing 11.

[0037] The turntable assembly 1 is provided with an outer ring cup placement position 15 and an inner ring cup placement position 16 arranged coaxially. The outer ring cup placement position 15 includes an inlet position 151, a mixing position 152, an incubation position 153, and an outlet position 154 arranged in sequence; the inner ring cup placement position 16 includes an inlet position 2 161, a mixing position 2 162, an ultrasonic position 163, and an outlet position 2 164 arranged in sequence, with inlet position 151 corresponding to inlet position 2 161, and outlet position 154 corresponding to outlet position 2 164. The first turntable 13 has multiple placement holes 131, which are respectively arranged one-to-one with inlet position 151, mixing position 152, incubation position 153, outlet position 154, inlet position 2 161, mixing position 2 162, ultrasonic position 163, and outlet position 2 164.

[0038] The number of working positions on the outer ring cup placement position 15 is equal to the number of working positions on the inner ring cup placement position 16, and they are arranged in a one-to-one correspondence. The working positions include mixing position one 152, mixing position two 162, incubation position 153, and ultrasonic position 163. There are two or more incubation positions 153, all arranged adjacent to each other; there are two or more ultrasonic positions 163, all arranged adjacent to each other. In this embodiment, taking three incubation positions 153, three ultrasonic positions 163, and five cup sleeves 14 as an example, the entry position one 151, mixing position one 152, three incubation positions 153, and one exit position one 154 are arranged evenly spaced circumferentially; the entry position two 161, mixing position two 162, three ultrasonic positions 163, and one exit position two 164 are also arranged evenly spaced circumferentially. The pressure cup sleeve 14 is set on the top cover 12, and the pressure cup sleeve 14 is set directly above the mixing position 152, the mixing position 2 162 and the three ultrasonic positions 163.

[0039] The top cover 12 has at least four through holes, which are respectively set and connected to the input position 151, output position 154, input position 2 161 and output position 2 164, so as to facilitate the insertion and removal of the sample tube 7.

[0040] A rotary drive assembly 2 is disposed at the lower part of the turntable assembly 1 and is used to drive the first turntable 13 to rotate relative to the housing 11 and the top cover 12 around its axis. Specifically, the rotary drive assembly 2 includes a rotary motor 21, a main shaft 22, a first drive pulley 23, a first timing belt 24, and a first driven pulley 25. The first drive pulley 23 is disposed on the drive shaft of the rotary motor 21, the first driven pulley 25 is disposed at the lower part of the main shaft 22, the upper part of the main shaft 22 is connected to the first turntable 13, and the first timing belt 24 is wound around the first drive pulley 23 and the first driven pulley 25. The rotary motor 21 drives the main shaft 22 to rotate around its axis in a stop-and-go manner via the first timing belt 24, thereby driving the first turntable 13 to rotate in a stop-and-go manner.

[0041] The lifting assembly 3 includes a lifting drive device and a lifting plate 33. The lifting plate 33 is located below the housing 11 and is slidably fitted onto the outside of the main shaft 22. The lifting drive device includes a lifting motor 31 and a first lead screw nut 32. The first lead screw nut 32 is threaded onto the drive shaft of the lifting motor 31 and is fixedly connected to the lifting plate 33. In this embodiment, the lifting motor 31 drives the lifting plate 33 to move up and down through the first lead screw nut 32, which helps to ensure the accuracy of the vertical movement distance of the lifting plate 33. In other embodiments, the lifting drive device may also be a cylinder or a hydraulic cylinder.

[0042] The mixing assembly 4 includes a mixing drive device 41 and two mixing mechanisms 42. Both mixing mechanisms 42 are mounted on the lifting plate 33, and are positioned below mixing position one 152 and mixing position two 162, respectively. When the lifting plate 33 moves upward, it drives the two mixing mechanisms 42 to move upward synchronously, allowing the upper parts of the mixing mechanisms 42 to pass through the bottom wall of the housing 11 and extend into their respective mixing positions. The mixing mechanism 42 lifts the sample tube 7 from its lower part, causing the upper part of the sample tube 7 to abut against the pressure cup sleeve 14. With the cooperation of the pressure cup sleeve 14 and the mixing mechanism 42, the sample tube 7 is fixed. Afterward, the mixing mechanism 42 performs an oscillating mixing action on the sample tube 7.

[0043] The mixing mechanism 42 includes a telescopic coupling, an eccentric mixing shaft 423, and a mixing seat 424. The telescopic coupling is movably inserted into the lifting plate 33, with both its upper and lower ends protruding from the upper and lower sides of the lifting plate 33. When the lifting plate 33 rises, it can extend the telescopic coupling; when the lifting plate 33 descends, it can shorten the telescopic coupling. The telescopic coupling includes a convex coupling 421 and a concave coupling 422 that can move relative to each other, with the upper part of the convex coupling 421 slidably connected to the lower part of the concave coupling 422. The eccentric mixing shaft 423 connects the mixing seat 424 and the upper part of the concave coupling 422, and the mixing seat 424 is used to connect to the lower part of the sample tube 7. The mixing drive device 41 can drive the telescopic coupling to rotate around its axis, thereby driving the mixing seat 424 to rotate eccentrically through the eccentric mixing shaft 423. The mixing drive device 41 includes a mixing motor 411, a second driving pulley 412, a second synchronous belt 413, and two second driven pulleys 414. The second driving pulley 412 is mounted on the drive shaft of the mixing motor 411, and the two second driven pulleys 414 are respectively mounted on the lower part of the U-shaped couplings 421 of the two mixing mechanisms 42. The second synchronous belt 413 is wound around both the second driving pulley 412 and the two second driven pulleys 414. The mixing motor 411 drives both mixing mechanisms 42 to perform mixing operations simultaneously via the second synchronous belt 413.

[0044] The ultrasonic component 5 includes an ultrasonic controller 51 and ultrasonic heads 52. The number of ultrasonic heads 52 is equal to the number of ultrasonic positions 163 and they are arranged in a one-to-one correspondence. This embodiment takes three ultrasonic heads 52 as an example. The three ultrasonic heads 52 are all set on the lifting plate 33 and are respectively set directly below the three ultrasonic positions 163. When the lifting plate 33 moves upward, it can drive the three ultrasonic heads 52 to move upward synchronously, so that the upper part of the ultrasonic head 52 passes through the bottom wall of the housing 11 and extends into the corresponding ultrasonic position 163. The ultrasonic head 52 lifts the sample tube 7 from the bottom, so that the upper part of the sample tube 7 abuts against the pressure cup sleeve 14. With the cooperation of the pressure cup sleeve 14 and the ultrasonic head 52, the sample tube 7 is fixed. After that, the ultrasonic controller 51 controls the three ultrasonic heads 52 to emit ultrasonic waves to perform ultrasonic treatment on the sample tube 7.

[0045] In this embodiment, the outer cup placement position 15 is used to place the first sample tube 7 to meet the first testing requirements. The sample tube 7 in the outer cup placement position 15 is first subjected to the oscillation and mixing operation of the mixing mechanism 42 and then incubated three times to achieve macroscopic mixing, which will not cause cell rupture and ensure that the component concentration of each part of the sample aspirated in subsequent tests is consistent, thus avoiding errors caused by different sampling positions.

[0046] In this embodiment, the inner cup placement position 16 is used to place the second sample tube 7 to meet the second testing requirements. The sample tube 7 in the inner cup placement position 16 is first subjected to the oscillation and mixing operation of the mixing mechanism 42 to achieve macroscopic mixing, ensuring that the component concentration of each part of the sample aspirated for subsequent testing is consistent; then, ultrasonic treatment is used to solve microscopic aggregation or lysis problems, such as breaking cells and lysing pathogens to release nucleic acids. The combination of mixing and ultrasonic treatment can improve the comprehensiveness of sample processing.

[0047] In other embodiments, the ultrasound position and the incubation position can be interchanged, such that the inner cup position is used to place the first sample tube to meet the first testing requirement, and the outer cup position is used to place the second sample tube to meet the second testing requirement.

[0048] In this embodiment, an electric heating film (not shown in the figure) and a first heat insulation layer (not shown in the figure) are provided on the bottom wall and / or side wall of the housing 11. The first heat insulation layer is located on the outward side of the electric heating film, so that the heat emitted by the electric heating film is maintained inside the housing for a long time, and the outside of the housing will not be burned due to excessive temperature. A second heat insulation layer (not shown in the figure) is provided on the outer side and / or inner side of the top cover 12. The electric heating film and heat insulation layer in this embodiment enable the sample tube 7 to undergo corresponding pretreatment in a constant temperature environment.

[0049] See Figures 5 to 7 and combined Figure 1 The sample preprocessing module 10 also includes a clamping component 6, which is mounted on the lifting plate 33 and moves up and down synchronously with it.

[0050] The clamping assembly 6 includes a fixed plate 61, a clamping drive device 62, a first gripper 63, and a second gripper 64. The clamping drive device 62 is mounted on the fixed plate 61. The first gripper 63 and the second gripper 64 are positioned opposite each other, forming two clamping openings 65. The clamping drive device 62 drives the first gripper 63 or the second gripper 64 to move closer or further apart. The two clamping openings 65 are respectively located directly below the first outlet 154 and the second outlet 164, used to simultaneously clamp the sample tubes 7 located within the first outlet 154 and the second outlet 164, facilitating subsequent unscrewing of the tube cap, aspiration of sample liquid, and tightening of the tube cap. At least two buffer pads 66 are provided within each clamping opening 65, positioned on opposite sides of the clamping opening 65. The buffer pads 66 are in direct contact with the sample tubes 7, helping to prevent damage to the sample tubes 7. The buffer pads 66 can be made of materials such as rubber, silicone, or sponge.

[0051] In one embodiment, one of the gripper 63 and the gripper 64 is movable, while the other is fixed. This embodiment uses the example of gripper 63 being movable and gripper 64 being fixed. The clamping drive device 62 drives gripper 63 to move closer to or further away from gripper 64, thereby clamping or releasing the clamping port 65. The clamping drive device 62 includes a clamping motor 621, a movable seat 622, a second lead screw nut 623, and a guide rail 624. The second lead screw nut 623 is threaded onto the drive shaft of the clamping motor 621. The guide rail 624 is mounted on a fixed plate 61. The movable seat 622 is slidably connected to the guide rail 624, and the second lead screw nut 623 is mounted on the movable seat 622. Gripper 63 is connected to the movable seat 622. The clamping motor 621 drives the lead screw shaft to rotate, thereby causing the movable seat 622 to move linearly.

[0052] In another embodiment, both gripper one and gripper two are movably configured, and the clamping drive device simultaneously drives gripper one and gripper two to move closer to or further away, thereby achieving clamping or releasing at the clamping port.

[0053] Example of an isothermal nucleic acid amplification and detection device: See Figure 8 and combined Figure 2 This embodiment provides an isothermal nucleic acid amplification and detection device, including a sample module 20, a pipette tip module 40, a reagent module 50, an auxiliary reagent module 60, a mixing module 70, a centrifugation module 80, a waste bin 90, an incubation and interpretation module 100, and the sample pretreatment module 10 of the above embodiment.

[0054] For ease of explanation, Figure 8 The left and right directions in the image are set as the X direction. Figure 8 The vertical direction is set to the Y direction.

[0055] The sample module 20 is located on one side of the sample pretreatment module 10 in the Y direction, the incubation interpretation module 100 is located on one side of the sample pretreatment module 10 in the X direction, the pipette tip module 40 is located on one side of the incubation interpretation module 100 in the Y direction, and the pipette tip module 40 is located on one side of the sample module 20 in the X direction, and the reagent module 50 is located between the incubation interpretation module 100 and the pipette tip module 40.

[0056] The auxiliary reagent module 60, the mixing module 70, the centrifugation module 80, and the waste bin 90 are all located between the incubation and interpretation module 100 and the reagent module 50. The mixing module 70 is located between the auxiliary reagent module 60 and the centrifugation module 80, and the waste bin 90 is located between the auxiliary reagent module 60 and the incubation and interpretation module 100.

[0057] The mixing module 70 is provided with a first mixing position C1, a second mixing position C2, and a transfer position C3. The centrifugation module 80 is provided with two centrifugation positions C4.

[0058] The reagent module 50 includes multiple reaction tubes 150 containing reagents and multiple reaction tube caps.

[0059] The incubation interpretation module 100 includes a second turntable with inner and outer rings of cup placement positions. The inner ring of the second turntable includes, in sequence, an inner ring cup inlet position, seven inner ring incubation positions, and an inner ring cup outlet position; the outer ring of the second turntable includes, in sequence, an outer ring cup inlet position, five fiber optic positions, twenty outer ring incubation positions, and one outer ring cup outlet position.

[0060] The isothermal nucleic acid amplification and detection equipment also includes a capping gripper 30, a puncture and capping gripper 110, a pipetting arm 120, a first reagent transfer arm 130, and a second reagent transfer arm 140.

[0061] The capping gripper 30 moves back and forth between the sample module 20 and the sample preprocessing module 10 to transfer the sample tube 7 on the sample module 20 to the carry-in 151 or carry-in 161 in the sample preprocessing module 10. It is also used to unscrew or tighten the cap of the sample tube 7 located in the exit 154 or exit 164, and to move the sample tube 7 in the exit 154 or exit 164 back into the sample module 20.

[0062] The puncture and capping gripper 110 moves back and forth between the reagent module 50 and the mixing module 70. The puncture and capping gripper 110 punctures the coating on the opening of the reaction tube 150 in the reagent module 50 and grasps the reaction tube 150 to the transfer position C3 in the mixing module 70. The puncture and capping gripper 110 can also grasp the cap of the reaction tube in the reagent module 50 and install it onto the reaction tube 150 in the transfer position C3.

[0063] The pipette arm 120 moves back and forth between the pipette tip module 40, the auxiliary reagent module 60, the sample pretreatment module 10, the mixing module 70, and the trash can 90. The pipette arm 120 picks up the pipette tip from the pipette tip module 40, then sequentially aspirates the auxiliary reagent from the auxiliary reagent module 60, and the sample solution from sample tube 7 (outlet 154 or outlet 164 of the sample pretreatment module 10). Next, it drops the mixture into reaction tube 150 in transposition C3 of the mixing module 70. The pipette arm 120 can also dispose of the used pipette tip in the trash can 90.

[0064] The first reagent transfer arm 130 moves back and forth between the mixing module 70, the centrifugation module 80, and the incubation interpretation module 100. The first reagent transfer arm 130 is used to transfer the reaction tube 150 (with sample and auxiliary reagents added) in the intermediate transfer position C3 to the first mixing position C1 for the first mixing. The first reagent transfer arm 130 is also used to transfer the reaction tube 150 after the first mixing to the centrifugation module 80 for centrifugation. The first reagent transfer arm 130 is used to transfer the centrifuged reaction tube 150 to the inner ring inlet position of the incubation interpretation module 100, where the reaction tube 150 rotates with the second turntable to complete the first incubation.

[0065] The second reagent transfer arm 140 moves back and forth between the incubation and interpretation module 100, the mixing module 70, and the waste bin 90. The second reagent transfer arm 140 is used to transfer the reaction tube 150, which has completed the first incubation, from the inner ring outlet position to the second mixing position C2 of the mixing module 70 to complete the second mixing action. The second reagent transfer arm 140 is also used to transfer the reaction tube 150 to the outer ring inlet position of the second turntable. This reaction tube 150 rotates with the second turntable to sequentially complete the first interpretation, second incubation, second interpretation, third incubation, third interpretation, fourth incubation, fourth interpretation, fifth incubation, and fifth interpretation. Afterward, the second reagent transfer arm 140 transfers the reaction tube 150 from the outer ring outlet position into the waste bin 90.

[0066] The workflow of the isothermal nucleic acid amplification and detection equipment is as follows: The first step, sample pretreatment step: the capping gripper 30 grabs the sample tube 7 from the sample module 20 and transfers it to the input position 151 or input position 261 of the sample pretreatment module 10 for mixing, incubation or sonication, and then unscrews the cap of the sample tube 7 at the output position 154 or output position 264 to prepare for subsequent sample aspiration. The second step, reagent preparation: The puncture and capping gripper 110 punctures the coating on the opening of the reaction tube 150 of the reagent module 50 and grasps the reaction tube 150 to transfer it to the intermediate position C3; The third step, sample addition: The pipette arm 120 pierces the pipette tip of the tip module 40 and sequentially aspirates the auxiliary reagent from the auxiliary reagent module 60 and the sample solution from the sample pretreatment module 10, then drips them into the reaction tube 150 in the intermediate transfer position C3; the puncture and capping gripper 110 grabs the cap of the reaction tube in the reagent module 50 and presses it onto the reaction tube 150 in the intermediate transfer position C3 to seal the reaction tube 150; the first reagent transfer arm 130 sequentially transfers the reaction tube 150 containing the sample and reagents to the first mixing position C1 and the centrifugation position C4 for the first mixing and centrifugation; Step 4, Nucleic Acid Amplification: The first reagent transfer arm 130 transfers the centrifuged reaction tube 150 to the incubation and interpretation module 100 for the first incubation, first interpretation, second incubation, second interpretation, third incubation, third interpretation, fourth incubation, fourth interpretation, fifth incubation, and fifth interpretation; after the first incubation and before the first interpretation, the second reagent transfer arm 140 transfers the reaction tube 150 to the second mixing position C2 for the second mixing; Step 5: The second reagent transfer arm 140 picks up the interpreted reaction tube 150 and places it into the trash can 90; the cap screwing gripper 30 screws the sample tube cap back onto the sample tube 7 at either output position 154 or output position 164 of the sample pretreatment module 10, and then returns the sample tube 7 to its original position in the sample module 20.

[0067] In summary, through the above-described configuration, samples placed in the outer ring cup position 15 and the inner ring cup position 16 can be independently subjected to different sample pretreatments. The outer ring cup position 15 is used to place the first sample, which can achieve oscillation mixing and incubation pretreatment, while the inner ring cup position 16 is used to place the second sample, which can achieve oscillation mixing and ultrasonic pretreatment. This invention is compatible with two different sample testing needs and has the advantages of simple structure, small size, and flexible application to different scenarios.

[0068] Finally, it should be emphasized that the above are only preferred embodiments of the present utility model and are not intended to limit the present utility model. For those skilled in the art, the present utility model can have various changes and modifications. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A sample preprocessing module, comprising a turntable assembly, a rotation drive assembly, a lifting assembly, and a mixing assembly, wherein the turntable assembly is provided with an outer ring cup placement position, an inner ring cup placement position, and a first turntable; the rotation drive assembly drives the first turntable to rotate around its axis; the mixing assembly includes a mixing drive device and two mixing mechanisms; the mixing drive device drives the mixing mechanisms to rotate; and the lifting assembly drives the two mixing mechanisms to move up and down, characterized in that: The outer ring cup placement positions include sequentially arranged entry position one, mixing position one, incubation position one, and exit position one; the inner ring cup placement positions include sequentially arranged entry position two, mixing position two, ultrasonic position two, and exit position two; the first turntable has multiple placement holes, and the multiple placement holes are respectively arranged in a one-to-one correspondence with entry position one, mixing position one, incubation position one, exit position one, entry position two, mixing position two, ultrasonic position two, and exit position two. The two mixing mechanisms are respectively disposed below the first mixing position and the second mixing position; The sample preprocessing module also includes an ultrasound component, which includes an ultrasound controller and an ultrasound head. The ultrasound head is positioned below the ultrasound position. The ultrasound controller controls the ultrasound head to emit ultrasound waves, and the lifting component drives the ultrasound head to move up and down.

2. The sample preprocessing module according to claim 1, characterized in that: The turntable assembly includes a housing, a top cover, and at least three pressure cup sleeves. The first turntable is rotatably disposed inside the housing. The top cover covers the upper part of the housing. The top cover has at least four through holes, which are respectively corresponding to and connected to the first input position, the first output position, the second input position, and the second output position. The pressure cup sleeves are disposed on the top cover. The pressure cup sleeves are disposed above the first mixing position, the second mixing position, and the ultrasonic position.

3. The sample preprocessing module according to claim 2, characterized in that: The number of incubation sites is set to two or more, and all the incubation sites are arranged adjacent to each other; The number of ultrasound positions is set to two or more, and all ultrasound positions are arranged adjacent to each other. The number of ultrasound heads is equal to the number of ultrasound positions, and the ultrasound heads and ultrasound positions are set in a one-to-one correspondence.

4. The sample preprocessing module according to claim 2, characterized in that: An electric heating film and a first heat insulation layer are provided on the bottom wall and / or side wall of the housing, and the first heat insulation layer is provided on the outward side of the electric heating film; A second insulation layer is provided on the outer and / or inner sides of the top cover.

5. The sample preprocessing module according to claim 2, characterized in that: The lifting assembly includes a lifting drive device and a lifting plate. The lifting plate is disposed below the housing. The mixing mechanism and the ultrasonic head are both disposed on the lifting plate. The lifting drive device drives the mixing mechanism and the ultrasonic head to move up and down simultaneously through the lifting plate, so that the upper part of the mixing mechanism and the upper part of the ultrasonic head extend into the corresponding mixing position and the ultrasonic position, respectively.

6. The sample preprocessing module according to claim 5, characterized in that: The sample preprocessing module also includes a clamping assembly, which is disposed on the lifting plate and moves up and down with the lifting plate. The clamping assembly has two clamping ports, which are respectively disposed below the first output position and the second output position. The clamping assembly includes a clamping drive device, a first gripper and a second gripper. The first gripper and the second gripper are arranged opposite each other and form two clamping openings. The clamping drive device drives the first gripper and / or the second gripper to move closer or further apart.

7. The sample preprocessing module according to claim 5, characterized in that: The mixing mechanism includes a telescopic coupling, an eccentric mixing shaft, and a mixing seat. The telescopic coupling is movably inserted into the lifting plate, which can extend and retract the telescopic coupling. The eccentric mixing shaft is located on the upper part of the telescopic coupling, and the mixing seat is located on the upper part of the eccentric mixing shaft. The mixing drive device drives the telescopic coupling to rotate around its axis, thereby driving the mixing seat to rotate eccentrically through the eccentric mixing shaft.

8. An isothermal nucleic acid amplification and detection device, characterized in that: Includes the sample preprocessing module as described in any one of claims 1 to 7.

9. The isothermal nucleic acid amplification and detection device according to claim 8, characterized in that: The isothermal nucleic acid amplification and detection equipment also includes a sample module, a pipette tip module, a reagent module, an auxiliary reagent module, a mixing module, a centrifugation module, a waste bin, and an incubation and interpretation module; The sample module is located on one side of the sample pretreatment module in the Y direction, the incubation and interpretation module is located on one side of the sample pretreatment module in the X direction, the pipette tip module is located on one side of the incubation and interpretation module in the Y direction, and the pipette tip module is located on one side of the sample module, and the reagent module is located between the incubation and interpretation module and the pipette tip module. The auxiliary reagent module, the mixing module, the centrifugation module, and the waste bin are all located between the incubation interpretation module and the reagent module, with the mixing module located between the auxiliary reagent module and the centrifugation module, and the waste bin located between the auxiliary reagent module and the incubation interpretation module.

10. The isothermal nucleic acid amplification and detection device according to claim 9, characterized in that: The isothermal nucleic acid amplification and detection equipment also includes a capping gripper, a puncture and capping gripper, a pipetting arm, a first reagent transfer arm, and a second reagent transfer arm; The capping gripper moves back and forth between the sample module and the sample preprocessing module; The puncture capping gripper moves back and forth between the reagent module and the mixing module; The pipette arm moves back and forth between the pipette tip module, the auxiliary reagent module, the sample pretreatment module, the mixing module, and the waste bin; The first reagent transfer arm moves back and forth between the mixing module, the centrifugation module, and the incubation interpretation module; The second reagent transfer arm moves back and forth between the incubation and interpretation module, the mixing module, and the waste bin.

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