A tumor cell culture device for anti-tumor experiment

Abstract

The utility model discloses an anti -tumor experiment is with tumor cell culture device belongs to cell culture equipment technical field. The device includes the culture bin, and the inside bottom is equipped with the chute, and the mesa is fixedly connected with the moving block, and the moving block is slidably fitted in the chute, and the rack is fixedly connected in the moving block, and the gear is rotatably installed in the culture bin bottom and is engaged with two rack, and the handle is fixedly connected in one mesa. By pulling the handle, the synchronous symmetrical movement of two mesa can be realized. The device is also provided with the culture frame fixedly connected with the mesa, including the detachable support column, and the bottom end limit block is fitted in the mesa groove, and the locking and release of limit block can be realized through the cooperation of fixed column, torsional spring driven baffle and support block. The utility model realizes the convenient movement of culture platform through the gear and rack linkage mechanism, and designs the culture frame detachable stand column mechanism, effectively solves the taking and placing problem of culture dish, and ensures the stability of cell culture environment.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment technology, and in particular to a tumor cell culture device for anti-tumor experiments. Background Technology

[0002] In anti-tumor experiments, in vitro culture of tumor cells is a crucial foundational step for drug screening and mechanism studies. Cell culture devices, commonly known as cell culture chambers or culture chambers, are designed to provide a highly controlled, in vivo-mimicking environment for cell growth and proliferation. This requires maintaining constant temperature, humidity, carbon dioxide concentration, and sterility within the culture chamber.

[0003] To achieve and maintain this precise internal environment, culture chambers are typically designed as sealed structures, allowing researchers to perform necessary operations such as placing and removing culture dishes and changing solutions only through sealed access ports. In conventional designs, culture dishes are placed on multi-layered, fixed culture racks inside the chamber. As experimental throughput increases, the depth of the culture chamber and the number of racks also increase accordingly. At this point, the inherent inconvenience of this fixed rack structure becomes apparent.

[0004] When researchers need to manipulate culture dishes located deep within the culture chamber, they must extend their arms a considerable distance into the access opening. This posture is not only strenuous, but the significant arm movement near the opening easily disrupts airflow, increasing the risk of contamination of the internal environment by outside air. Furthermore, the long arm reach reduces precision and increases the risk of touching other culture dishes, potentially causing experimental accidents. Current technology fails to provide an effective structural solution to improve the accessibility of items deep within the culture chamber.

[0005] Therefore, this invention proposes a tumor cell culture device for anti-tumor experiments to address the shortcomings of existing technologies. Utility Model Content

[0006] In view of the problems in the existing anti-tumor experimental tumor cell culture devices, such as the inconvenience of operating the culture dishes deep inside the culture chamber, the cumbersome process of picking up and putting down the culture dishes, and the risk of contamination, the present invention aims to provide an anti-tumor experimental tumor cell culture device with an improved structure that can effectively solve the above problems.

[0007] This utility model provides a tumor cell culture device for anti-tumor experiments, comprising: a culture chamber, wherein the inner bottom surface of the culture chamber is provided with at least two sliding grooves, at least two platform surfaces, at least two moving blocks, at least two racks, as well as gears and handles.

[0008] The movable blocks are fixedly connected to the bottom end of the platform and slidably fitted into the slide groove, and the rack is fixedly connected to the upper surface of the bottom end of the movable block.

[0009] Furthermore, the gear is rotatably mounted on the bottom surface of the culture chamber and located between the two racks, meshing with each rack. The handle is fixedly connected to the outer wall of one of the platforms. This linkage between the gear and the racks on both sides allows pulling a single handle to drive both platforms to move synchronously outwards or inwards symmetrically, significantly improving the operability inside the culture chamber.

[0010] Preferably, the device further includes a culture rack, which is fixedly connected to the upper surface of the platform, and the culture rack includes at least one detachable support column.

[0011] Preferably, the tabletop has a groove, and a limiting block is fixedly connected to the bottom end of the detachable support column. The limiting block is pluggably fitted into the groove. The device also includes a fixed column, a baffle, a torsion spring, and a support block. The fixed column and the support block are both fixedly connected to the tabletop. The baffle is rotatably mounted on the fixed column. The torsion spring is sleeved on the fixed column and connected to the baffle, and is used to drive the baffle to abut against the support block and lock the limiting block.

[0012] Preferably, the device further includes a petri dish placed on the culture rack, and an operating block is fixed to one side of the periphery of the petri dish.

[0013] Preferably, a metal plate is fixedly connected to the inner wall of the culture chamber, and a heating wire is fixedly connected to the metal plate.

[0014] Preferably, the device further includes a sterile filtration module and a carbon dioxide cylinder, and the device also includes a gas delivery pipe, which connects the sterile filtration module to the interior of the culture chamber, and the gas delivery pipe connects the carbon dioxide cylinder to the interior of the culture chamber.

[0015] Preferably, the culture chamber has a glass window on the front and operation ports on both side walls, and the operation ports are sealed with airtight doors.

[0016] Preferably, a medical plastic shallow dish is placed on the inner bottom surface of the culture chamber.

[0017] This utility model has the following beneficial effects: 1. In this utility model, by setting a movable block that can slide in the chute, a rack fixed to the movable block, and a gear that meshes with the racks at both locations simultaneously, and by setting a handle on one side of the platform, the problem of difficult access and placement of culture dishes that are deep inside the culture chamber in the prior art is solved. It achieves the technical effect that pulling a handle can make the two culture platforms move out or retract symmetrically to the two operation ports at the same time, which greatly facilitates the operation of the experimental personnel.

[0018] 2. In this utility model, by setting a detachable support column on the culture rack, and using the cooperation between the limiting block and the groove, as well as the baffle driven by the torsion spring to automatically lock the limiting block, the problem of difficulty in horizontally picking up and placing culture dishes in the existing multi-layer culture rack due to the fixed column is solved. It achieves the technical effect of quickly unlocking and removing the support column and conveniently picking up the culture dish horizontally. The structure is simple and the locking is reliable, avoiding the risk of contamination during the picking and placing process. Attached Figure Description

[0019] Figure 1 This is a three-dimensional schematic diagram of a tumor cell culture device for anti-tumor experiments proposed in this utility model; Figure 2 This is a schematic diagram of the structure of the platform of the anti-tumor experimental tumor cell culture device proposed in this utility model; Figure 3 This is a schematic diagram of the structure of a culture dish support for an anti-tumor experimental tumor cell culture device proposed in this utility model; Figure 4 This is a schematic diagram of the gear rack structure of an anti-tumor experimental tumor cell culture device proposed in this utility model.

[0020] Legend: 1. Sterile filtration module; 2. Culture chamber; 3. Gas supply tube; 4. Carbon dioxide cylinder; 5. Airtight door; 6. Glass window; 7. Metal plate; 8. Heating wire; 9. Worktable; 10. Medical plastic shallow tray; 11. Handle; 12. Slide; 13. Culture dish; 14. Culture rack; 15. Operating block; 16. Fixing column; 17. Torsion spring; 18. Baffle; 19. Support block; 20. Limiting block; 21. Moving block; 22. Rack; 23. Gear. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0022] Example: Please refer to Figures 1 to 4 This utility model provides a tumor cell culture device for anti-tumor experiments, which aims to solve the problems in the prior art where it is difficult to pick up and put down culture dishes deep in the culture chamber, and the operation of multi-layer culture racks is inconvenient when picking up and putting down culture dishes.

[0023] like Figure 1 and Figure 2 As shown, the tumor cell culture device for anti-tumor experiments includes a culture chamber 2, with at least two grooves 12 on the inner bottom surface of the culture chamber 2, at least two platforms 9, at least two movable blocks 21 fixedly connected to the bottom end of the platform 9 respectively, and the movable blocks 21 slidably engaged with the grooves 12, at least two racks 22, the bottom end of the movable blocks 21 fixedly connected to the upper surface of the racks 22, and gears 23 rotatably mounted on the bottom surface of the culture chamber 2, the gears 23 being located between the two racks 22 and meshing with the two racks 22 respectively. The device also includes a handle 11, which is fixedly connected to the outer wall of one of the platforms 9.

[0024] like Figure 3 As shown, the device also includes a culture rack 14, which is fixedly connected to the upper surface of the tabletop 9. The culture rack 14 includes at least one detachable support column. The device also includes a culture dish 13, which is placed on the culture rack 14. An operating block 15 is fixed to one side of the outer periphery of the culture dish 13. A groove is provided on the tabletop 9. A limiting block 20 is fixedly connected to the bottom end of the detachable support column. The limiting block 20 is pluggably fitted into the groove. The device also includes a fixing column 16, a baffle 18, a torsion spring 17, and a support block 19. The fixing column 16 and the support block 19 are both fixedly connected to the tabletop 9. The baffle 18 is rotatably installed on the fixing column 16. The torsion spring 17 is sleeved on the fixing column 16 and connected to the baffle 18, and is used to drive the baffle 18 to abut against the support block 19 and lock the limiting block 20.

[0025] To solve the above-mentioned technical problems, the core of the technical solution in this embodiment lies in the linkage platform mechanism and the quick-release mechanism of the culture rack. Please refer to the following for details. Figure 1 , Figure 2 , Figure 3 and Figure 4The core structure is described in detail below: The linkage platform mechanism includes a gear 23, two racks 22, two moving blocks 21, two sliding grooves 12, two platform surfaces 9, and a handle 11. The gear 23 is rotatably mounted on the bottom surface of the culture chamber 2 and located between the two racks 22. The two racks 22 mesh with the gear 23 respectively. The bottom end of the moving block 21 is fixedly connected to the upper surface of the rack 22. The moving block 21 slides in the sliding groove 12 opened on the bottom surface of the culture chamber 2. The platform surface 9 is fixedly connected to the top end of the moving block 21. The handle 11 is fixedly connected to the outer wall of one of the platform surfaces 9. When the handle 11 is pulled, the rack 22 connected to it drives the gear 23 to rotate. The gear 23 then pushes the other rack 22 to move in the opposite direction, so that the two platform surfaces 9 move out or retract symmetrically towards the airtight doors 5 on both sides of the culture chamber 2. The quick-release mechanism of the culture rack includes the components fixed to the platform surface 9. The system includes a culture rack 14, at least one detachable support column of the culture rack 14, a limiting block 20 at the bottom of the detachable support column, a groove on the tabletop 9, a fixing column 16, a baffle 18, a torsion spring 17, and a support block 19. The limiting block 20 is pluggably fitted into the groove. The fixing column 16 and the support block 19 are both fixedly connected to the position of the tabletop 9 adjacent to the groove. The baffle 18 is rotatably installed on the fixing column 16. The torsion spring 17 is sleeved on the fixing column 16. One end of the torsion spring 17 is connected to the fixing column 16, and the other end is connected to the baffle 18. The torsion spring 17 drives the baffle 18 to abut against the support block 19. At this time, the baffle 18 locks the limiting block 20, locking the detachable support column. When it is necessary to remove the culture dish 13, the baffle 18 is manually opened to release the locking of the limiting block 20, and the detachable support column can be removed. The culture dish 13 can then be horizontally pulled out by the operating block 15 fixed to one side of the periphery of the culture dish 13.

[0026] Based on the above embodiments, the present invention may further include the following preferred technical solutions: In a preferred embodiment, in order to maintain a suitable temperature inside the culture chamber 2, a metal plate 7 is fixedly connected to the inner wall of the culture chamber 2, and a heating wire 8 is fixedly connected to the metal plate 7. The heating wire 8 generates heat and the heat is evenly diffused into the interior of the culture chamber 2 through the metal plate 7. As another preferred embodiment, in order to maintain the sterile and acid-base balance environment required for cell culture, the device also includes a sterile filter module 1 and a carbon dioxide cylinder 4 installed outside the culture chamber 2. One end of the gas supply pipe 3 is connected to the inside of the culture chamber 2, and the other end is connected to the sterile filter module 1 and the carbon dioxide cylinder 4 respectively. Outside air enters the culture chamber 2 after being sterilized by the sterile filter module 1, and the carbon dioxide cylinder 4 outputs carbon dioxide through the gas supply pipe 3 to regulate the culture environment. As another preferred embodiment, in order to facilitate observation of the culture status and aseptic operation, a glass window 6 is installed on the front of the culture chamber 2, and operation ports are opened on both side walls of the culture chamber 2. An airtight chamber door 5 that can be opened and closed is installed on the operation port. The airtight chamber door 5 can be used with sealed gloves. As another preferred embodiment, in order to maintain the humidity inside the culture chamber 2, a medical plastic shallow dish 10 is placed on the inner bottom surface of the culture chamber 2. The medical plastic shallow dish 10 is used to hold sterile distilled water.

[0027] Working principle: During operation, the experimenter pulls the handle 11 fixed to the outer wall of one of the platform surfaces 9. The platform surface 9 moves outward through the moving block 21 at its bottom, which drives the rack 22 below it. Since the rack 22 meshes with the gear 23 in the middle, the movement of the rack 22 forces the gear 23 to rotate. The rotating gear 23 meshes with the other rack 22 at the same time, which in turn pushes the rack 22, the moving block 21 above it, and the platform surface 9 to move in opposite outward directions. Throughout the process, the two moving blocks 21 slide and engage in the sliding groove 12 on the bottom surface of the culture chamber 2, so that the two platform surfaces 9 and the culture rack 14 above them move outward synchronously and symmetrically towards the airtight doors 5 on both sides, making it convenient for the experimenter to pick up and put down the culture dish 13.

[0028] When it is necessary to place or remove the petri dish 13, in the default locked state, the torsion spring 17 is sleeved on the fixed column 16, and its elastic force drives the baffle 18 to abut against the support block 19. At this time, the baffle 18 is just locked in the limiting block 20 at the bottom of the detachable support column, so that the support column is firmly fixed in the groove of the table 9. The experimenter needs to manually open the baffle 18 to overcome the elastic force of the torsion spring 17 and separate the baffle 18 from the limiting block 20. At this time, the limiting block 20 is no longer locked, and the detachable support column can be lifted upward and moved away. After the support column is removed, the experimenter can use the operating block 15 fixed on one side of the periphery of the petri dish 13 to horizontally pull out or place the petri dish 13 from the culture rack 14.

[0029] During the culture process, the sterile filter module 1 installed on the top of the culture chamber 2 filters and sterilizes the incoming air. The carbon dioxide cylinder 4 delivers carbon dioxide into the culture chamber 2 through the gas supply pipe 3. The metal plate 7 on the inner wall of the culture chamber 2 works in conjunction with the heating wire 8 to provide a uniform and stable temperature inside the culture chamber 2. The medical plastic shallow dish 10 placed on the bottom maintains the internal humidity by evaporating sterile distilled water. Experimenters can observe the internal situation through the glass window 6 on the front or open the airtight chamber doors 5 on both sides of the side walls to operate the equipment. Together, they provide a sterile, constant temperature, constant humidity and controllable gas stable environment for cell culture.

[0030] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A tumor cell culture device for anti-tumor experiments, comprising: The culture chamber (2) has at least two grooves (12) on its inner bottom surface. At least two countertops (9); At least two movable blocks (21) are fixedly connected to the bottom end of the table (9) and the movable blocks (21) are slidably engaged with the slide groove (12). At least two racks (22), the bottom end of the moving block (21) is fixedly connected to the upper surface of the racks (22); Its features are, Gear (23), the gear (23) is rotatably mounted on the bottom surface of the culture chamber (2), and the gear (23) is located between the two racks (22) and meshes with the two racks (22) respectively; The device also includes a handle (11) which is fixedly connected to the outer wall of one of the tabletops (9).

2. The tumor cell culture device for anti-tumor experiments according to claim 1, characterized in that, The device also includes a culture rack (14), which is fixedly connected to the upper surface of the table (9), and the culture rack (14) includes at least one detachable support column.

3. The tumor cell culture device for anti-tumor experiments according to claim 2, characterized in that, The tabletop (9) has a groove, and the bottom end of the detachable support column is fixedly connected to a limiting block (20). The limiting block (20) is pluggably fitted into the groove. The device also includes a fixed column (16), a baffle (18), a torsion spring (17), and a support block (19). The fixed column (16) and the support block (19) are both fixedly connected to the tabletop (9). The baffle (18) is rotatably installed on the fixed column (16). The torsion spring (17) is sleeved on the fixed column (16) and connected to the baffle (18) to drive the baffle (18) to abut against the support block (19) and lock the limiting block (20).

4. The tumor cell culture device for anti-tumor experiments according to claim 2, characterized in that, The device also includes a petri dish (13) placed on the culture rack (14); an operating block (15) is fixed to one side of the periphery of the petri dish (13).

5. The tumor cell culture device for anti-tumor experiments according to claim 1, characterized in that, A metal plate (7) is fixedly connected to the inner wall of the culture chamber (2), and a heating wire (8) is fixedly connected to the metal plate (7).

6. The tumor cell culture device for anti-tumor experiments according to claim 1, characterized in that, The device also includes a sterile filtration module (1) and a carbon dioxide cylinder (4). The device also includes a gas delivery pipe (3), which connects the sterile filtration module (1) to the interior of the culture chamber (2) and the carbon dioxide cylinder (4) to the interior of the culture chamber (2).

7. The tumor cell culture device for anti-tumor experiments according to claim 1, characterized in that, The culture chamber (2) has a glass window (6) on the front and operation ports on both sides of the culture chamber (2). The operation ports are sealed with airtight doors (5).

8. The tumor cell culture device for anti-tumor experiments according to claim 1, characterized in that, A medical plastic shallow dish (10) is placed on the inner bottom surface of the culture chamber (2).

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