An apparatus for collecting venom from small chelonoides reptiles

Abstract

The utility model discloses a device for fast collection small size scorpion venom, including collection groove and collection pad. The four vertical sides of the collection groove are composed of a sealed baffle and an inserted electrified grid, wherein the sealed baffle is further composed of an integrated three-sided sealed fixed baffle on the outside and a vertical sealed door. The inner side of the three-sided sealed fixed baffle is provided with a horizontal sealed clamping groove of the same height for inserting the electrified grid, a vertical sealed clamping groove is provided at the entrance of the electrified grid, and a vertical sealed door is provided. When in use, the vertical door is lowered to form a collection groove with the electrified grid, which is four-sided sealed and open at the top. The collection pad is composed of replaceable insulating collection film and flexible sponge. When in use, talcum powder is applied to the inner wall of the collection groove to prevent insects from escaping, and then the collection groove is placed on the flexible sponge pad with the collection film laid thereon. Through electric stimulation, the insects produce attack behavior, and the venom is enriched on the collection film, and then the venom is efficiently collected through the solvent leaching method.

Description

Technical Field

[0001] This utility model relates to the field of venom collection technology for small stinging reptiles, specifically a device for collecting venom from small stinging reptiles. Background Technology

[0002] Small insects, such as red imported fire ants and tropical fire ants, as well as small bee species, have developed a unique ecological defense strategy: injecting venom through stings. The released venom has significant value in basic research and biological and pharmaceutical applications. Current venom collection techniques are limited to some larger bees, whose stingers are typically 1-10 mm long, easily penetrating skin and membranes; their venom is mostly proteinaceous; and the amount of venom released by each individual is large. Larger insects can be stimulated to release venom using electrical stimulation techniques and collected directly in glass bottles. However, for other smaller stinging insects, whose stingers are less than 0.5 mm long, it is difficult to penetrate membranes, resulting in a small amount of venom released. This makes it impossible to collect large quantities of venom using existing methods; only very small amounts can be collected manually, which is extremely inefficient. Utility Model Content

[0003] The purpose of this invention is to provide a device for collecting venom from small stinging insects. By applying appropriate electrical stimulation, a large amount of venom from small stinging insects can be collected, thus overcoming the shortcomings of existing small insect venom collection technologies.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a device for collecting venom from small stinging insects, comprising a collection tank, characterized in that: the collection tank is based on an integral three-sided sealed and fixed baffle as the main frame, and each of the three-sided sealed and fixed baffles has a horizontal sealing slot of the same height on its inner side. The two opposite opening ends of the three-sided sealed and fixed baffles are provided with two vertical sealing slots to fix the pull-down sealing door, which together with the electrically conductive grid inserted into the horizontal sealing slots form a collection tank that is open at the top and sealed on all sides.

[0005] A replaceable insulating sampling film is placed close to the bottom of the energized grid. To ensure that the sampling film is in close contact with the conductive wires of the energized grid, a flexible sponge is used to gently press the replaceable insulating sampling film onto the energized grid plate. An external current controller is connected to the energized grid.

[0006] Preferably, the electrified grille is installed inside a three-sided sealed and fixed baffle, forming a sealed groove with the pull-down sealing door that is closed at the bottom and on all four sides.

[0007] Preferably, the energized grid plate is electrically connected to the current controller.

[0008] Preferably, the external current controller includes a circuit control unit.

[0009] Preferably, the replaceable insulating collection film includes conventional solvent-resistant stable dielectric films such as alumina film, polytetrafluoroethylene film, and polynatride film.

[0010] Preferably, the solvent used for venom collection is n-hexane or water.

[0011] Compared with the prior art, the practical effects of this utility model are as follows:

[0012] 1. Apply talcum powder along the inner edge of the three-sided sealed baffle and the sliding sealing door to prevent insects from escaping. Insert the electrified grid plate into the sealing groove and close the sliding door to form a sealed groove. Gently press the sealed groove onto a flexible sponge with a replaceable insulating collection film laid on it, and then place the insects to be extracted into the collection groove. After the insects are evenly dispersed, adjust the DC voltage between 30-60V using the controller to induce the stinging insects to bite the conductive wire with their mandibles, and apply venom to the surface of the replaceable insulating collection film with their stingers. Collect the venom from the surface of the replaceable insulating collection film by cleaning with organic solvents. The voltage and stimulation time can be freely controlled according to the venom collection situation, and the important components of this device are all detachable, making cleaning and carrying more convenient, effectively improving practicality and solving the problem of low efficiency in traditional manual venom collection.

[0013] 2. This invention allows for the selection of the most suitable and replaceable insulating collection membrane material. Based on the physicochemical properties of different insect venoms and their varying solubility in common organic solvents (such as acetone and n-hexane) and inorganic solvents (such as water), a suitable elution solvent can be chosen to achieve rapid collection of large quantities of venom. Subsequent physicochemical property analysis can be performed using gas chromatography and liquid chromatography. Furthermore, the close contact between the replaceable insulating collection membrane and the conductive wire effectively avoids the problem of uncertain venom release locations caused by the small size of the insect and the short stinger. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the specific structure of the electrified grid of this utility model;

[0016] Figure 3 This is a schematic diagram of the external circuit controller of this utility model;

[0017] Figure 4 This is a diagram showing the result of using this utility model.

[0018] In the diagram: 1. Collection tank; 11. Integrated three-sided sealed fixing baffle; 12. Three-sided horizontal sealing slot; 13. Two inner vertical sealing slots; 14. Pull-down sealing door; 2. Electrified grid; 21. Electrified grid plate; 211. Positive electrode conductive wire of the electrified grid plate; 212. Negative electrode conductive wire of the electrified grid plate; 22. Current controller; 221. Voltage control knob; 222. Circuit board; 223. Transformer; 3. Replaceable insulating collection membrane; 4. Flexible sponge pad; 4-1. Attack behavior of fire ants after being electrocuted by the electrified grid; 4-2. Fire ant venom collected by the replaceable insulating collection membrane; 4-3. Gas chromatographic analysis of venom (4-3-1. Venom extracted from fire ants by direct solvent soaking (containing epidermal hydrocarbon impurities); 4-3-2 to 4-3-5. Venom extracted by this device in experiments, 4 replicates). Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figures 1-3A device for collecting venom from small stinging reptiles includes a collection tank 1. The collection tank 1 has an outer integrated three-sided sealed baffle 11 as its main frame. Each of the three-sided sealed baffle has a horizontal sealing slot 12 of the same height on its inner side. In use, an electrified grid plate 21 is horizontally inserted into the sealing slot 12 and then fixed by a pull-out sealing door 14 through two inner vertical sealing slots 13. Together with the electrified grid 2 inserted into the horizontal slots, this forms a collection tank 1 that is open at the top and sealed on all sides. Before use, a layer of talcum powder is evenly applied to the inner wall of the collection tank 1 to control the escape of the reptiles. A replaceable insulating collection film 3 is placed tightly against the electrified grid 2. To ensure that the replaceable insulating collection film 3 is tightly attached to the conductive wires 211 and 212 of the electrified grid, the electrified grid plate 21 is placed face-up on a thick flexible sponge pad 4, and then the replaceable insulating collection film 3 is gently pressed onto the conductive wires 211 and 212. After the insects are placed in the collection tank 1, the power is turned on. The 220V voltage is reduced to 48V DC by the transformer 223, supplying power to the external current controller 22 of the electrified grid 2. The current first passes through the circuit board 222 and then enters the voltage control knob 221. By adjusting the voltage control knob 221, the voltage of the electrified grid 2 is controlled between 30 and 60V, causing the crawling insects to release venom on the replaceable insulating collection membrane 3 after being electrically stimulated. When a sufficient amount of venom has accumulated on the replaceable insulating collection membrane 3, the membrane is removed, and the replaceable insulating collection membrane 3 is eluted with a suitable elution solvent according to the type of venom collected. This method can greatly increase the efficiency of collecting venom from small stinging crawling insects, reducing the time and labor costs of manual collection.

[0021] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0022] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for collecting venom from small stinging reptiles, comprising a collection tank (1), characterized in that: The collection tank (1) is mainly framed by an integrated three-sided sealing and fixing baffle (11) on the periphery. Each of the three-sided sealing and fixing baffles is provided with a horizontal sealing slot (12) of the same height on the inner side. The two opposite sides of the three-sided sealing and fixing baffles are provided with two vertical sealing slots (13) to fix the vertical sealing door (14). Together with the power grid (2) inserted into the horizontal sealing slot (12), it forms a collection tank (1) with an open top and sealed on all sides. A replaceable insulating collection film (3) is placed close to the bottom of the power grid (2). In order to ensure that the collection film is close to the electrical conductor wire of the power grid, the replaceable insulating collection film (3) is gently pressed onto the power grid plate (21) using a flexible sponge (4). The power grid (2) is connected to an external current controller (22).

2. The device for collecting venom from small stinging reptiles according to claim 1, characterized in that: The energized grid plate (21) is electrically connected to the external current controller (22).

3. The device for collecting venom from small stinging reptiles according to claim 1, characterized in that: The external current controller (22) has a built-in circuit control unit.

4. The device for collecting venom from small stinging reptiles according to claim 1, characterized in that: The replaceable insulating collection film (3) includes solvent-resistant stable dielectric films such as alumina film, polytetrafluoroethylene film, and polynatate film.

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