A whole-cycle universal plant tissue culture bottle

Abstract

The utility model discloses a whole cycle general plant tissue culture bottle belongs to tissue culture bottle technical field, and this tissue culture bottle includes first bottle body, second bottle body and bottle lid, is equipped with first protrusion on first bottle body, is equipped with first recess on second bottle body, and first recess includes guide groove and limit groove, when second bottle body is relative to first bottle body and moves upward, guide groove moves upward along first protrusion, is equipped with sealing ring on second bottle body, and sealing ring is pressed on the outer wall of first bottle body under the elastic force of it. The utility model discloses tissue culture bottle can adapt to herbaceous, xylophytic, medicinal and so on multiple type tissue culture seedling, guarantee the sealing property simultaneously, and the sampling port and the material supplementing port satisfy the sampling and the material supplementing need, do not need to open bottle lid, reduce the pollution rate, set up the optical filter in bottle lid, can insert different wavelength optical filter, adapt to different tissue culture seedling light quality demand, satisfy space adaptation, environmental control, aseptic operation, light quality demand simultaneously, and all parts can be put into high pressure sterilization pot and carry out high pressure sterilization without disassembling.

Description

Technical Field

[0001] This utility model belongs to the field of tissue culture bottle technology, specifically relating to a universal plant tissue culture bottle for the entire life cycle. Background Technology

[0002] As the core carrier for the growth of tissue culture seedlings, plant tissue culture bottles need to balance "versatility" and "adaptability" to meet the growth needs of different types of tissue culture seedlings (such as herbaceous flowers, woody fruit trees, medicinal plants, and ornamental seedlings). Existing universal tissue culture bottles are typically made of glass and plastic, and come in various heights and capacities to accommodate different types of seedlings, increasing purchase and management costs. Some tissue culture bottles feature a "vent plug + filter membrane" cap structure for easy ventilation, but opening the cap is required for sampling or refilling, leading to potential contamination of the seedlings. To accommodate the different light quality requirements of seedlings, some tissue culture bottles incorporate LED lights inside the cap, increasing cap costs and preventing direct autoclaving. Utility Model Content

[0003] The technical problem solved by this utility model is to provide a universal plant tissue culture bottle that can meet the seedling needs of different types of tissue culture seedlings throughout the entire growth cycle.

[0004] Technical solution: To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0005] A universal plant tissue culture bottle for the entire life cycle includes a first bottle body, a second bottle body fitted over the first bottle body, and a cap connected to the second bottle body. The outer wall of the first bottle body has a first protrusion, and the inner wall of the second bottle body has a first groove corresponding to the first protrusion. The first groove includes a guide groove and a limiting groove. When the second bottle body moves upward relative to the first bottle body, the guide groove moves upward along the first protrusion. The second bottle body has a sealing ring, which presses against the outer wall of the first bottle body under its elastic force.

[0006] Furthermore, the guide groove is vertically arranged, and the limiting groove is perpendicular to the guide groove.

[0007] Furthermore, the bottle cap is provided with a breathable module, which includes a breathable membrane layer, a breathable valve disposed outside the breathable membrane layer, and a dust cover disposed outside the breathable valve.

[0008] Furthermore, the bottle cap is provided with a first functional port and a second functional port, the first functional port is provided with a sealing plug, and the second functional port is provided with a sealing cap.

[0009] Furthermore, the bottle cap is provided with a filter, and the filter is provided with a first through hole corresponding to the first functional port, a second through hole corresponding to the second functional port, and a third through hole corresponding to the breathable module.

[0010] Furthermore, the bottom of the first bottle is provided with an anti-slip pad, and the bottle cap is provided with a stacking groove corresponding to the bottom of the first bottle.

[0011] Furthermore, the inner wall of the first bottle is provided with a textured layer, and the bottom wall of the first bottle is provided with a second protrusion, which is annular.

[0012] Furthermore, the first bottle body has a magnifying observation strip on its side wall, and the second bottle body has a label strip on its outer side wall.

[0013] Furthermore, the first bottle body is provided with a first scale line, and the second bottle body is provided with a second scale line.

[0014] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0015] 1. By setting a first bottle body and a second bottle body that is movably connected to the first bottle body, a first protrusion is set on the outer wall of the first bottle body, and a corresponding first groove is set on the inner wall of the second bottle body to facilitate movement and fixation. After the second bottle body moves up and down, the height of the entire tissue culture bottle can be adjusted. A sealing ring is set on the second bottle body so that the tissue culture bottle can be adapted to various types of tissue culture seedlings such as herbaceous, woody, and medicinal plants, while ensuring airtightness.

[0016] 2. A breathable module is set up to meet the different breathability requirements of succulents (high breathability), herbaceous flowers (medium breathability), and woody plants (low breathability). The sampling port and feeding port meet the needs of sampling and feeding without opening the bottle cap, thus reducing the contamination rate.

[0017] 3. The bottle cap is equipped with a filter, which can be inserted with different wavelength filters to adapt to the light quality requirements of different tissue culture seedlings. The filter can be sterilized and reused, which is low cost.

[0018] 4. The bottle body is coated and the inner wall of the bottle is textured. The bottom wall of the inner wall of the bottle is provided with a second protrusion to reduce adhesion and improve the seedling separation efficiency.

[0019] 5. A magnification observation bar is provided, allowing observation of tiny seedlings without the need for an external magnifying glass;

[0020] 6. It simultaneously meets the requirements of space adaptability, environmental control, aseptic operation, and light quality. All parts can be placed into the autoclave for autoclaving without disassembly, thus improving work efficiency. Attached Figure Description

[0021] Figure 1This is a schematic diagram of the structure of an embodiment of the present utility model;

[0022] Figure 2 yes Figure 1 Enlarged view of point A in the middle;

[0023] Figure 3 yes Figure 1 Enlarged view at point B in the middle;

[0024] Figure 4 This is a schematic diagram of the first bottle structure in the embodiment;

[0025] Figure 5 This is a schematic diagram of the second bottle structure in the embodiment;

[0026] Figure 6 This is a schematic diagram of the bottle cap structure in an embodiment;

[0027] Figure 7 This is a schematic diagram of the breather valve structure in an embodiment;

[0028] Figure 8 This is a schematic diagram of the filter structure in the embodiment;

[0029] Figure 9 This is a magnified schematic diagram of the strip structure in the embodiment. Detailed Implementation

[0030] The present invention will be further illustrated below with reference to specific embodiments. The embodiments are implemented based on the technical solution of the present invention. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0031] like Figure 1 , Figure 2 , Figure 3 and Figure 5As shown, a universal plant tissue culture bottle for the entire life cycle includes a first bottle body 1, a second bottle body 2, and a bottle cap 3. The first bottle body 1, the second bottle body 2, and the bottle cap 3 are all made of existing high-transparency and anti-aging PP (polypropylene) material. Both the inner and outer surfaces are coated with a nano-silica composite coating. The bottle body has a visible light transmittance of ≥92%, can withstand high-pressure sterilization at 121℃ (deformation rate ≤2% after 50 sterilization cycles), and has an impact resistance 5 times that of traditional glass (bottle body transportation breakage rate ≤1%). The coating can reduce condensation (condensation area reduced by 65%) and inhibit microbial adhesion (miscellaneous bacteria adhesion rate reduced by 45%). The first bottle body 1 is a cylindrical shape with an opening at the top. A first protrusion 11 is provided on the outer wall of the first bottle body 1, located near the upper end. A second bottle body 2 is fitted over the first bottle body 1. The second bottle body 2 is a cylindrical shape with openings at both the top and bottom. The inner diameter of the second bottle body 2 is larger than the outer diameter of the first bottle body 1. A first groove 21 is provided on the inner wall of the second bottle body 2, corresponding to the first protrusion 11. The first groove 21 includes a guide groove 211 and a limiting groove 212. The guide groove 211 is vertically positioned, and the limiting groove 212... The groove 212 is perpendicular to the guide groove 211. Multiple limiting grooves 212 are arranged at equal intervals from top to bottom. When the second bottle 2 moves upward relative to the first bottle 1, the guide groove 211 moves upward along the first protrusion 11. After moving to the predetermined position, the second bottle 2 is rotated so that the first protrusion 11 enters the limiting groove 212 of the corresponding height, and the second bottle 2 is fixed at that height. If it is necessary to move the second bottle 2 up or down, the second bottle 2 is rotated in the opposite direction so that the first protrusion 11 enters the guide groove 211. At this time, the second bottle 2 can be moved up and down. The inner wall of the second bottle 2 is also provided with a second groove 22, and a sealing ring 4 is provided in the second groove 22. The sealing ring 4 has a circular cross-section, and the shape of the second groove 22 is hemispherical. Most of the sealing ring 4 is accommodated in the second groove 22. When the second bottle 2 is fitted over the first bottle 1, the sealing ring 4 is pressed against the outer wall of the first bottle 1 under its elastic force. The sealing ring 4 is made of food-grade silicone material, which can ensure the airtightness between the first bottle 1 and the second bottle 2. There is no risk of water ingress during sterilization (moisture content ≤5% after sterilization), and the contamination rate is controlled below 3%. The height of the entire tissue culture bottle can be adjusted by pulling the second bottle 2. The first bottle 1 has a first scale line, and the second bottle 2 has a second scale line (not shown in the scale line diagram). The first scale line is blue, from 0cm to 12cm, and the second scale line is red, from 12cm to 30cm. Adjustable height tissue culture bottles are suitable for different tissue culture seedlings: ① Herbaceous flowers (such as petunias and gerberas): adjust the height to 12-15cm, the shallow space avoids crowding of seedlings; ② Woody fruit trees (such as pears and apples): adjust the height to 20-30cm to meet the longitudinal growth of the root system (maximum root length 25cm); ③ Medicinal plants (such as ginseng and dendrobium): adjust the height to 15-20cm to balance the growth of the above-ground and underground parts.This allows for segmented adjustment of the height (12-30cm) and corresponding adjustment of the volume of the tissue culture bottle, which can simultaneously accommodate different types of tissue culture seedlings, such as herbaceous flowers (shallow bottom), woody fruit trees (tall cylinder), and medicinal plants (medium height). This solves the limitation of the traditional "one type of bottle for one type of seedling" and reduces equipment procurement costs (enterprises do not need to purchase 3-4 types of special bottles, reducing procurement costs by 60%).

[0032] like Figure 1 , Figure 6 and Figure 7As shown, the bottle cap 3 is connected to the second bottle body 2. The upper outer wall of the second bottle body 2 is provided with a standard tissue culture bottle thread, and the bottle cap 3 is provided with a corresponding internal thread. The bottle cap 3 is threadedly connected to the second bottle body 2. A venting module 5 is provided in the middle of the bottle cap 3. The venting module 5 is cylindrical in shape and adopts a three-layer composite venting structure, including a venting membrane layer 51, a venting valve 52, and a dust cover 53. The venting membrane layer 51 is located in the innermost layer and includes an outer cylindrical shell and a venting membrane in the inner ring of the cylindrical shell. In this embodiment, a 0.22μm PTFE breathable membrane (sterilization resistant and good air permeability) is used. The air permeable valve 52 is located outside the breathable membrane layer 51. The air permeable valve 52 can be an existing fan-shaped valve or a manual butterfly valve; in this embodiment, a fan-shaped valve is used. The air permeable valve 52 includes a housing 521, a fixed sealing plate 522, a movable plate 523, and a central shaft 524. The housing 521 is cylindrical and integrally connected to the cylindrical shell of the breathable membrane layer 51. An airflow channel 520 for air circulation is formed in the middle of the housing 521. The fixed sealing plate 522 is a sector-shaped plate, and its arc-shaped edge is connected to the inner wall of the housing 521. The central shaft 524 is located at the center of the housing 521 and is rotatably connected to the fixed sealing plate 522. The movable plate 523 is connected to the central shaft 524 and is higher than the fixed sealing plate 522. The movable plate 523 is also a sector-shaped plate, and the inner side wall of the housing 521 is provided with an annular groove corresponding to the arc-shaped plate of the movable plate 523. When the central shaft 524 rotates, it drives the movable plate 523 to rotate. When the movable plate 523 rotates above the fixed sealing plate 522, it overlaps the fixed sealing plate 522. In this embodiment, the area of ​​the movable plate 523 occupies two-fifths of the area of ​​the middle circle of the shell 521. The working area of ​​the movable plate 523 is equal to the area of ​​the fixed sealing plate 522. Thus, the area of ​​the airflow channel 520 is at its minimum of one-fifth of the area of ​​the middle circle (when the movable plate 523 rotates to a position completely offset from the fixed sealing plate 522) and at its maximum of three-fifths (when the movable plate 523 rotates to a position completely overlapping the fixed sealing plate 522). The air permeability can be manually adjusted through the vent valve 52. Different air permeability can be adapted to different tissue culture seedlings. The dust cover 53 is set outside the vent valve 52. The dust cover 53 adopts a cap body that is closed at one end. The dust cover 53 covers the top and is fixed to the top of the vent valve 52 by its own elasticity. The dust cover 53 can be covered to prevent moisture when the tissue culture bottle is sterilized. The dust cover 53 can be opened when in use. The venting valve 52, in conjunction with the venting membrane layer 51, allows for an adjustable air permeability range of 50-150 mL / min. Different air permeability levels can be adapted to different tissue culture seedlings: ① Succulents (e.g., Crassulaceae): Air permeability adjusted to 120-150 mL / min to reduce humidity inside the bottle; ② Herbaceous flowers (e.g., Phalaenopsis orchids): Air permeability adjusted to 80-100 mL / min to balance humidity and CO2 supply; ③ Woody plants (e.g., walnuts): Air permeability adjusted to 50-80 mL / min to reduce water evaporation. This structure can control the contamination rate below 3% while meeting different humidity requirements.

[0033] likeFigure 1 and Figure 6 As shown, the bottle cap 3 has a first functional port 301 and a second functional port 302, which are located on both sides of the venting module 5. Both the first functional port 301 and the second functional port 302 are circular. In this embodiment, the first functional port 301 serves as a sampling port. The first functional port 301 has a diameter of 5 mm and is equipped with a sealing plug 61. The sealing plug 61 is a silicone plug, which can be pierced with a sterile syringe to draw out the culture medium in the tissue culture bottle (without opening the cap). The lower end of the sealing plug 61 is duckbill-shaped and its material is elastic, so it automatically springs back to seal after the syringe is punctured. The second functional port 302 serves as a feeding port. The second functional port 302 has an external threaded tube, and a sealing cap 62 is threaded onto the external threaded tube. Opening the sealing cap 62 allows connection to a sterile feeding tube for precise addition of hormones and nutrients (such as sucrose mother liquor and growth hormones) to avoid contamination from opening the cap. For example, when adding GA3 to Dendrobium officinale tissue culture seedlings, adding it through the feeding port can effectively reduce the contamination rate (from 10% to 2%).

[0034] like Figure 6 and Figure 8 As shown, a light filter 7 is provided inside the bottle cap 3, and a third groove is provided on the inner circumference of the bottle cap 3. The light filter 7 is a circular piece, and its outer circumference enters the third groove to be fixed inside the bottle cap 3. The light filter 7 has a first through hole 71, a second through hole 72, and a third through hole 73. The first through hole 71 corresponds to the first functional port 301, the second through hole 72 corresponds to the second functional port 302, and the third through hole 73 corresponds to the ventilation module 5, so as not to affect the sampling, feeding, and ventilation functions. There are various types of light filters 7, and different wavelength filters (such as blue light filters and red light filters) can be inserted to adapt to the light quality requirements of different tissue culture seedlings: ① Phalaenopsis orchid: insert a blue light filter (wavelength 450-470nm) to promote flower bud differentiation; ② Dendrobium officinale: insert a red light filter (wavelength 620-660nm) to increase polysaccharide content; when no light quality adjustment is required, a transparent sheet can be inserted without affecting light transmission. The filters can be sterilized and reused (≥50 times) at a low cost (≤2 yuan per filter).

[0035] like Figure 1 , Figure 4 and Figure 6 As shown, the bottom of the first bottle 1 is provided with an anti-slip pad 12, which is a ring-shaped silicone anti-slip pad to prevent the culture bottle from sliding on the culture rack; the bottle cap 3 is provided with a support block 13, the height of the support block 13 is higher than the height of the venting module 5, and a stacking groove 131 is formed on the support block 13. The stacking groove 131 corresponds to the bottom of the first bottle 1. When the tissue culture bottles are stacked up and down, the bottom of the upper tissue culture bottle is placed on the stacking groove 131 of the lower bottle cap 3, so that the tissue culture bottles can be stacked in multiple layers.

[0036] like Figure 4As shown, the inner wall of the first bottle 1 is provided with a textured layer 14, and the textured layer 14 is arrayed with micro-convex textures. The micro-convex textures adopt existing strip textures or spot textures, and the height of the micro-convex textures is 0.1 mm, which guides the culture medium to be evenly distributed and reduces adhesion. The bottom wall of the first bottle 1 is provided with a second protrusion 15, which is ring-shaped and has three lines. The height of the second protrusion 15 is 0.5 cm, which improves the seedling separation efficiency during subculture.

[0037] like Figure 9 As shown, the first bottle 1 has a magnifying observation strip 81 on its side wall. The magnifying observation strip 81 uses an existing card-type magnifying glass. A third groove corresponding to the magnifying observation strip 81 is located at one-third of the height of the outer side wall of the first bottle 1. The magnifying observation strip 81 is pasted into the third groove. The magnifying observation strip 81 is made of PVC and can be bent along the outer side wall of the first bottle 1. It provides a magnification of 2x, allowing observation of small seedlings inside the bottle (such as orchid protocorms or strawberry tissue culture seedling roots) without the need for an external magnifying glass. The second bottle 2 has a label strip (not shown in the figure) on its outer side wall. The label strip is pasted on the outer side wall of the second bottle 2 and uses an alcohol-resistant label for easy recording of tissue culture seedling type, inoculation date, and culture stage.

[0038] Work process

[0039] 1. Initial Adjustment: Adjust the parameters of the culture bottle according to the type of tissue culture seedlings—① Height Adjustment: If you need to cultivate apple rootstock seedlings (requires a height of 25cm), extend the telescopic section of the bottle to 25cm; ② Aeration Adjustment: For example, when cultivating succulents, rotate the bottle cap vent valve to the "high aeration" setting (150mL / min), at which point the airflow channel area is at its maximum; ③ Light Quality Adjustment: For example, when cultivating Phalaenopsis orchids, insert a blue light filter.

[0040] 2. Sterilization and Inoculation: Fill the bottle with the corresponding culture medium (e.g., apple rooting medium, succulent plant proliferation medium), close the dust cover 53 and the feeding port, and sterilize in an autoclave (121℃, 20 minutes). After cooling, inoculate the tissue culture seedlings in a clean bench. After inoculation, open the dust cover 53, adjust the air permeability to the target value, insert the corresponding filter 7, and place the bottle on a culture rack (temperature 23-27℃, light 2000-3000 lx).

[0041] 3. Cultivation Process Control: During cultivation, the nutrients in the culture medium (such as sucrose concentration, nitrogen, phosphorus, and potassium content) are tested regularly through the sampling port. If supplementation is needed, sterile mother liquor is injected through the supplementation port. The growth status of the tissue culture seedlings (such as protocorm proliferation and root length) can be observed through the magnified observation strip 81 without opening the cap. The slightly convex texture of the bottle guides the condensed water to flow down the bottle wall, avoiding affecting the seedlings.

[0042] 4. Subculture and Transplanting: Once the culture reaches the target stage (e.g., proliferation of flower buds, rooting of fruit trees), open the bottle cap 3 and quickly separate the seedlings using the second protrusion 15 on the bottom ring for subculture or transplanting. After cleaning the culture bottle (it can be cleaned with a regular brush; the slightly raised texture does not affect cleanliness), replace worn parts (e.g., the breathable membrane layer 51, the sealing plug 61), and it can be reused. The cost per use is only 0.2-0.3 yuan.

[0043] Feasibility analysis

[0044] (I) Technical Feasibility

[0045] 1. Mature materials and processes: The core material, "high-transparency anti-aging PP + nano-silica coating", has been widely used in the medical and food fields (such as baby bottles and sterile syringes). Its sterilization resistance and light transmittance have been verified over a long period of time. The telescopic structure adopts a sliding groove connection (which can be achieved by existing injection molding processes, with a mold cost of about 80,000 yuan). The sealing performance has passed standard testing (air leakage rate ≤0.1% after sterilization), and there is no need to develop new processes.

[0046] 2. Reliable functional modules: The 0.22μm PTFE breathable membrane (Millipore, USA), silicone sealing plug (Wacker, Germany), and filter (customized by a domestic optical company) are all commercially mature products, and the air permeability, sealing performance, and light transmittance can be precisely controlled. Small-batch tests show that when cultivating Phalaenopsis orchid tissue culture seedlings, the light transmittance reaches 92%, and the flower bud differentiation rate increases by 25%. When cultivating apple rootstock seedlings, the root length reaches 22cm, and the rooting rate increases by 18%. The technical effects meet expectations.

[0047] 3. Adaptability verification: Experiments on 10 common tissue culture seedlings (Phalaenopsis orchid, rose, apple, pear, ginseng, dendrobium, succulent, strawberry, potato, gerbera) showed that this design can meet the growth requirements of all types of seedlings, the contamination rate is controlled below 3%, the subculture efficiency is increased by 30%-40%, and there are no obvious adaptation problems.

[0048] (II) Economic Feasibility

[0049] 1. Controllable cost: The production cost of a single bottle is approximately 12-18 yuan (material cost 6-10 yuan, processing fee 2-3 yuan, functional component 4-5 yuan). Compared with imported special tissue culture bottles (such as German Greiner bottles, 30-50 yuan per bottle), the cost is reduced by 50%-70%. After being reused 50 times, the cost per use is only 0.24-0.36 yuan, which is lower than that of traditional glass bottles (0.5-1 yuan) and ordinary plastic bottles (0.3-0.5 yuan), demonstrating significant cost advantages due to economies of scale.

[0050] 2. Significant Benefits: For enterprises producing 10 million tissue culture seedlings annually, using this design of culture bottles will: ① reduce the contamination rate from 10% to 3%, reducing losses by 700,000 seedlings per year (equivalent to an additional 560,000 yuan in revenue at 0.8 yuan per seedling); ② increase space utilization by 140%, allowing for an additional 7 million seedlings in production capacity without expansion (an additional 5.6 million yuan in revenue); ③ reduce labor costs by 20% (reducing the number of workers by 2), saving 160,000 yuan annually; the total annual revenue increase is 6.32 million yuan, with an investment payback period of approximately 2 months.

[0051] 3. Clear market demand: The domestic tissue culture seedling market exceeds 100 billion yuan, covering multiple fields such as flowers, fruit trees, medicinal plants, and ornamental seedlings. Seedling companies (such as Yunnan flower bases, Shandong fruit tree seedling companies, and Zhejiang medicinal plant laboratories) have an urgent need for "universal and efficient" tissue culture bottles. This design can cover more than 90% of tissue culture scenarios, with a large market capacity and broad promotion prospects.

[0052] (III) Operational Feasibility

[0053] 1. Easy to use: All adjustment functions (height, air permeability, filter) require no professional skills. Ordinary tissue culture technicians can operate them proficiently after 10 minutes of training. For example, height adjustment only requires rotating and stretching the bottle (completed in 3 seconds); air permeability adjustment is done by rotating the valve (completed in 1 second); and sampling is done by penetrating the sealing plug with a syringe (completed in 5 seconds). The operation process is simple and easy to understand.

[0054] 2. Simple maintenance: Culture flasks can be cleaned using conventional methods (water + neutral detergent + brush), and the micro-textured and expandable structure does not affect cleaning; easily damaged parts (ventilated membrane, sealing plug) can be replaced individually (cost ≤ 1 yuan / piece), eliminating the need to replace the entire culture flask; filters can be reused through autoclaving, resulting in low maintenance costs. In large-scale production, one technician can maintain 2000 culture flasks, demonstrating high work efficiency.

[0055] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A universal plant tissue culture bottle for the entire life cycle, characterized in that, The first bottle (1) includes a first bottle body (1), a second bottle body (2) fitted outside the first bottle body (1), and a bottle cap (3) connected to the second bottle body (2). The first bottle body (1) has a first protrusion (11) on its outer side wall, and the second bottle body (2) has a first groove (21) corresponding to the first protrusion (11) on its inner side wall. The first groove (21) includes a guide groove (211) and a limiting groove (212). When the second bottle body (2) moves upward relative to the first bottle body (1), the guide groove (211) moves upward along the first protrusion (11). The second bottle body (2) has a sealing ring (4), which presses against the outer wall of the first bottle body (1) under its elastic force.

2. The universal plant tissue culture bottle according to claim 1, characterized in that, The guide groove (211) is vertically arranged, and the limiting groove (212) is perpendicular to the guide groove (211).

3. The universal plant tissue culture bottle according to claim 1, characterized in that, The bottle cap (3) is provided with a breathable module (5), which includes a breathable membrane layer (51), a breathable valve (52) disposed outside the breathable membrane layer (51), and a dust cover (53) disposed outside the breathable valve (52).

4. The universal plant tissue culture bottle for the entire life cycle according to claim 3, characterized in that, The bottle cap (3) is provided with a first functional port (301) and a second functional port (302). The first functional port (301) is provided with a sealing plug (61), and the second functional port (302) is provided with a sealing cap (62).

5. The universal plant tissue culture bottle for the entire life cycle according to claim 4, characterized in that, The bottle cap (3) is provided with a filter (7), and the filter (7) is provided with a first through hole (71) corresponding to the first functional port (301), a second through hole (72) corresponding to the second functional port (302) and a third through hole (73) corresponding to the breathable module (5).

6. The universal plant tissue culture bottle according to claim 1, characterized in that, The bottom of the first bottle body (1) is provided with an anti-slip pad (12), and the bottle cap (3) is provided with a stacking groove (131) corresponding to the bottom of the first bottle body (1).

7. The universal plant tissue culture bottle according to claim 1, characterized in that, The inner wall of the first bottle body (1) is provided with a textured layer (14), and the bottom wall of the first bottle body (1) is provided with a second protrusion (15), which is annular.

8. The universal plant tissue culture bottle according to claim 1, characterized in that, The first bottle (1) has a magnifying observation strip (81) on its side wall, and the second bottle (2) has a label strip on its outer side wall.

9. The universal plant tissue culture bottle according to claim 1, characterized in that, The first bottle body (1) is provided with a first scale line, and the second bottle body (2) is provided with a second scale line.

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