Chinese cabbage tissue culture device with light splitting function

Abstract

The utility model relates to a kind of white cabbage tissue culture equipment with light splitting function, including cultivation box;Still including the door of rotating installation in the front side of cultivation box, the bottom of cultivation box is equipped with two sliding grooves one, sliding groove one is slidably connected with sliding member, two sliding members are fixed with mounting frame, motor is installed in mounting frame, the output end of motor is connected with the rotating rod of vertical arrangement, rotating rod outside is fixed with three horizontal arrangement's tray, multiple clamping grooves for placing culture container are set up in tray surface, rotating rod outside is also fixed with three light source diffusion plate;The novel is through rotating tray and 360 ° light splitting illumination cooperation, eliminate the length of one-way light caused by deviation, improve seedling straightness and consistency;Combining adjustable multi-band LED aperture and light diffusion plate, according to callus, differentiation, rooting stage dynamic precision light supplement, improve light efficiency, optimize development, shorten cycle, reduce vitrification.

Description

Technical Field

[0001] This utility model relates to the technical field of cabbage tissue culture equipment, specifically a cabbage tissue culture equipment with a spectrophotometric function. Background Technology

[0002] The Chinese cabbage tissue culture equipment is an experimental or production device used for Chinese cabbage tissue culture. Its core feature is the integration of spectrophotometry, which allows for spectral control of the light source, providing specific wavelengths of light for the tissue culture process. This device promotes efficient growth and differentiation of Chinese cabbage tissues. Different wavelengths of light (such as red, blue, and far-red light) have significant effects on plant photosynthesis, morphogenesis, and flowering induction. Spectrophotometric control optimizes the growth status of Chinese cabbage tissue culture seedlings, improving tissue culture efficiency and survival rate. Precise light environments can reduce abnormal phenomena such as vitrification and yellowing seedlings, improving seedling quality. In scientific research and breeding applications, it can be used to study the effects of different light qualities on Chinese cabbage gene expression, metabolites, and stress resistance, assisting in molecular breeding or functional gene research. In agricultural biotechnology companies, it is used for large-scale, standardized production of high-quality virus-free or transgenic Chinese cabbage seedlings.

[0003] In common Chinese cabbage tissue culture equipment, light is usually provided by a fixed light source at the top or on one side. This unidirectional lighting method easily leads to a significant phototropic growth response in Chinese cabbage seedlings, specifically manifested as stems bending towards the light source, excessive elongation, internode elongation, and leaf slant, which are known as "excessive growth" or "uneven crown". This problem is mainly affected by the following factors: First, photoreceptors in plants (such as phototropin and cryptochrome) are sensitive to blue light. When light shines from only one side, the receptors are asymmetrically activated on the cell membrane, causing auxin (IAA) to be unevenly distributed on the shaded side of the shoot tip or hypocotyl. The cells on the shaded side elongate significantly faster than those on the illuminated side, causing the organ to bend towards the light source. Second, traditional tissue culture racks are mostly static structures with fixed positions of culture bottles, making it impossible to change the angle of light exposure. Prolonged unidirectional light exposure causes cabbage seedlings to grow continuously tilted in one direction, making it difficult to form an upright and compact ideal plant shape. Furthermore, most equipment uses LED light panels with a single red-blue light ratio, and the light intensity is unevenly distributed, with excessive light in areas close to the light source and insufficient light in areas far away, further exacerbating the growth differences. In addition, the light transmittance and placement density of the tissue culture containers themselves (such as Erlenmeyer flasks or test tubes) also affect the actual light reception effect. When densely arranged, adjacent containers block each other, creating local shadow areas and inducing more complex phototropic responses. This morphological abnormality caused by unidirectional light not only reduces the uniformity of the appearance of tissue culture seedlings, but also weakens their mechanical strength and physiological robustness, leading to difficulties in seedling recovery and a decrease in survival rate during subsequent transplanting. This seriously affects the efficiency and economic benefits of factory seedling production. Therefore, solving the problem of unidirectional light is one of the key technical bottlenecks in improving the quality of Chinese cabbage tissue culture. Existing tissue culture racks generally use a static tray with LED light panels fixed to the top or side walls. This structure cannot change the direction of light received by the plants, nor can it achieve dynamic spectral adaptation, resulting in unstable quality of tissue culture seedlings. Utility Model Content

[0004] To address the shortcomings of existing technologies, this utility model provides a cabbage tissue culture device with a spectral splitting function. By using a rotating tray in conjunction with 360° spectral splitting illumination, it avoids excessive elongation of the seedlings. Multi-band LEDs and a diffuser plate achieve precise supplemental lighting, improving efficiency and promoting growth while reducing vitrification. This solves the common problem of unidirectional light in tissue culture causing excessive elongation and uneven crowning of cabbage seedlings, affecting uniformity and transplant survival, mainly due to photoreceptor response, static placement, and uneven light intensity.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cabbage tissue culture device with a spectrophotometric function, comprising a culture box; and a door rotatably mounted on the front side of the culture box. Two sliding grooves are provided at the bottom of the culture box, and sliding components are slidably connected within these grooves. A mounting frame is fixed to both sliding components. A motor is installed inside the mounting frame, and the output end of the motor is connected to a vertically arranged rotating rod. Three horizontally arranged trays are fixed to the outside of the rotating rod. Multiple slots for placing culture containers are provided on the surface of each tray. Three light source diffusers are also fixed to the outside of the rotating rod, with each diffuser positioned directly above its corresponding tray. The top of the incubator has a sliding groove 2, and a slider is slidably connected in the sliding groove 2. The lower end of the slider is connected to the upper end of the rotating rod through a bearing. Three LED light rings 1 are installed on the inner side wall of the incubator, and three trays correspond to the three LED light rings 1. LED light rings 2 are installed on the inner surface of the door. The LED light rings 1 and 2 together form a continuous 360° beam-splitting illumination structure around the trays.

[0006] Furthermore, both LED aperture one and LED aperture two are composed of red, blue, far-red and white LED chips, and the light intensity of each LED band can be independently adjusted to achieve dynamic spectral control for different tissue culture stages of Chinese cabbage.

[0007] Furthermore, the light source diffuser plate is made of a light-transmitting polymer material, and its lower surface has a microstructure texture, which is used to evenly diffuse the light emitted by the LED aperture onto the entire surface of the culture container on the corresponding tray.

[0008] Furthermore, the motor is a low-speed adjustable stepper motor, which drives the rotating rod to rotate the tray at a constant speed of 0.01 to 0.5 revolutions per minute, thereby eliminating the phototropic elongation phenomenon caused by unidirectional light on plants.

[0009] Furthermore, the mounting frame can be pulled and moved in the front and back direction by the cooperation of the sliding part and the sliding groove, and the front side of the mounting frame is provided with a built-in handle for operation and maintenance of the rotating mechanism without disassembling the motor.

[0010] Furthermore, the connection structure between the slider in the second sliding groove and the bearing at the upper end of the rotating rod can provide vertical support during rotation and allow free rotation, ensuring that the three-layer pallet remains coaxial and stable without shaking during long-term operation.

[0011] Furthermore, after the chamber door is closed, it forms a sealed cavity with the incubator. LED aperture one and LED aperture two are connected when the chamber door is closed, together forming a complete ring light source.

[0012] Compared with the prior art, the technical solution of this application has the following beneficial effects: 1. By combining the rotating tray with the 360° continuous beam-splitting illumination structure, the problem of excessive phototropism in cabbage seedlings caused by unidirectional light is avoided, which improves the morphological uniformity and uprightness of tissue culture seedlings and lays a good foundation for improving the survival rate of subsequent transplanting. 2. By using independently adjustable multi-band LED apertures (red light, blue light, far-red light, white light) combined with a uniform light diffusion plate, dynamic and precise spectral supply is achieved for different tissue culture stages of Chinese cabbage (such as callus induction, bud differentiation, and rooting). This improves light energy utilization efficiency, optimizes physiological development, shortens the culture cycle, and reduces abnormal phenomena such as vitrification. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a partial three-dimensional structural diagram of the present invention; Figure 3 This is a three-dimensional structural diagram of the location of the built-in handle of this utility model; Figure 4 This is a three-dimensional structural diagram of the location of the LED aperture of this utility model; Figure 5 This is a three-dimensional structural diagram of the location of the tray in this utility model; Figure 6 This is a three-dimensional structural diagram of the location of the second LED aperture of this utility model.

[0014] In the diagram: 1. Incubator; 2. Door; 3. Mounting frame; 4. Sliding groove one; 5. Built-in handle; 6. Motor; 7. Rotating rod; 8. Tray; 9. Light source diffuser plate; 10. Sliding groove two; 11. Slider; 12. LED aperture one; 13. LED aperture two. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figures 1-6 This embodiment of a cabbage tissue culture device with a spectrophotometric function includes a culture box 1; it also includes a door 2 rotatably installed on the front side of the culture box 1. The bottom of the culture box 1 has two sliding grooves 4, and sliding parts are slidably connected in the sliding grooves 4. A mounting frame 3 is fixed on both sliding parts. A motor 6 is installed inside the mounting frame 3. The output end of the motor 6 is connected to a vertically arranged rotating rod 7. Three horizontally arranged trays 8 are fixed on the outside of the rotating rod 7. The surface of the tray 8 has multiple slots for placing culture containers. Three light source diffuser plates 9 are also fixed on the outside of the rotating rod 7. The three light source diffuser plates 9 are respectively located directly above the corresponding tray 8.

[0017] In this embodiment, the rotating tray 8 and 360° beam-splitting illumination work together to prevent the cabbage seedlings from growing too long due to unidirectional light, thereby improving morphological uniformity and uprightness. At the same time, the independently adjustable multi-band LED aperture and uniform light diffusion plate enable dynamic and precise supplemental lighting for stages such as callus induction, bud differentiation, and rooting, thereby improving light efficiency, optimizing the development process, shortening the cycle, and reducing vitrification.

[0018] Please see Figures 1-6 In this embodiment, to ensure that the cabbage seedlings receive uniform light, do not grow crooked or leggy, and grow neatly and upright, making transplanting easier, a sliding groove 10 is provided at the top inside the cultivation box 1. A slider 11 is slidably connected inside the sliding groove 10, and the lower end of the slider 11 is connected to the upper end of the rotating rod 7 through a bearing. Three LED light rings 12 are installed on the inner side wall of the cultivation box 1, and three trays 8 correspond one-to-one with the three LED light rings 12. An LED light ring 23 is installed on the inner surface of the box door 2. The LED light rings 12 and 13 together form a continuous 360° beam-splitting illumination structure around the trays 8. The light source diffuser plate 9 is made of a light-transmitting polymer material, and its lower surface has a microstructure texture, which is used to evenly diffuse the light emitted by the LED light rings onto the entire surface of the cultivation container on the corresponding tray 8. The motor 6 is a low-speed adjustable stepper motor, which drives the rotating rod 7 to rotate the trays 8 at a speed of 0.01 to 0.5 rpm, thereby eliminating the phototropic elongation phenomenon caused by unidirectional light.

[0019] In this embodiment, a low-speed adjustable stepper motor is used to drive the rotating rod 7 and the tray 8 to rotate at a constant speed of 0.01 to 0.5 revolutions per minute, so that the culture container rotates slowly and continuously, effectively eliminating the phototropic elongation phenomenon caused by unidirectional light. The LED light ring 13 on the inner side of the box door 2 and the three LED light rings 12 on the inner wall of the cultivation box 1 together form a 360-degree continuous beam-splitting illumination structure around the tray 8, providing a uniform light source in all directions. The light source diffuser plate 9 is made of a light-transmitting polymer material, and its lower surface has a microstructure texture, which can diffuse the light emitted by the LED softly and evenly onto the entire surface of the culture container, significantly improving the uniformity of light illumination and the efficiency of light energy utilization, thereby improving the morphological uniformity, stem and leaf uprightness and physiological health of the cabbage tissue culture seedlings.

[0020] It should be noted that the mounting frame 3 moves back and forth by sliding the sliding member and sliding groove 4. The mounting frame 3 has a built-in handle 5 on the front side, which is used to pull out the rotating mechanism for operation and maintenance without disassembling the motor 6. The connection structure between the slider 11 in the sliding groove 10 and the bearing at the upper end of the rotating rod 7 can provide vertical support and allow free rotation during rotation, ensuring that the three-layer tray 8 remains coaxial and stable without shaking during long-term operation. The mounting frame 3 can move back and forth by sliding the sliding member and sliding groove 4, which is convenient for the entire rotating mechanism to be removed for operation or maintenance. The built-in handle 5 is located on the front side of the mounting frame 3, which is convenient for users to hold and pull without disassembling the motor 6. The connection structure between the slider 11 in the sliding groove 10 and the bearing at the upper end of the rotating rod 7 provides stable vertical support and allows the rotating rod 7 to rotate freely during equipment operation, effectively ensuring the coaxiality and stability of the three-layer tray 8 during long-term operation, avoiding shaking or deviation, and improving the reliability of the equipment and the continuity of the tissue culture process.

[0021] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 6 In this embodiment, in order to achieve intelligent light distribution according to different cultivation stages, save power and increase efficiency, promote rapid seedling growth and reduce the likelihood of vitrification, both LED aperture 12 and LED aperture 23 in this embodiment are composed of red, blue, far-red and white LED chips. The light intensity of each LED band can be independently adjusted to dynamically regulate the spectrum for different tissue culture stages of Chinese cabbage. After the box door 2 is closed, it forms a sealed cavity with the cultivation box 1. LED aperture 12 and LED aperture 23 are connected when the box door 2 is closed, together forming a complete ring light source.

[0022] In this embodiment, both LED aperture 12 and LED aperture 2 13 integrate red, blue, far-red, and white LED chips. The light intensity of each band can be adjusted independently to achieve dynamic and precise supply of light spectrum for different culture stages such as callus induction, bud differentiation, and rooting of Chinese cabbage. After the box door 2 is closed, it forms a sealed cavity with the incubator 1. At the same time, LED aperture 12 and LED aperture 2 13 are seamlessly connected to form a complete ring light source, ensuring continuous and uniform illumination, effectively improving light energy utilization efficiency, promoting healthy and rapid growth of seedlings, reducing abnormal phenomena such as vitrification, and taking into account both energy saving and tissue culture quality.

[0023] It should be noted that both LED aperture 12 and LED aperture 2 13 are electrically connected to the controller. The controller has pre-stored spectral parameters suitable for the callus induction, bud differentiation and rooting stages of Chinese cabbage. Users can select the corresponding mode through the touch screen or external devices to realize the automatic adjustment of the LED light intensity of each band.

[0024] The working principle of the above embodiments is as follows: In use, first close the door 2 to form a sealed cavity in the incubator 1. Simultaneously, the LED ring 13 inside the door 2 automatically connects with the three LED rings 12 on the inner wall of the incubator 1, forming a complete 360-degree ring light source surrounding the tray 8. Both LED rings 12 and 13 contain red, blue, far-red, and white LED chips, and the light intensity of each band can be independently adjusted. Appropriate spectral combinations can be configured according to the tissue culture stage of the cabbage, such as callus induction, bud differentiation, or rooting dynamics, to achieve precise and efficient supplemental lighting. Then, the motor 6 starts, acting as a low-speed adjustable stepper motor, uniformly driving the rotating rod 7 at a speed of 0.01 to 0.5 revolutions per minute. This drives the three trays 8 and their culture containers to rotate slowly and continuously, preventing the plants from developing phototropism due to fixed-direction light exposure. During rotation, the slider 11 in the sliding groove 10 is connected to the upper end of the rotating rod 7 via a bearing, providing stability. The vertical support allows for free rotation, ensuring the long-term coaxial and stable operation of the three-layer tray 8 without shaking. Meanwhile, three light source diffuser plates 9, fixed on the rotating rod 7, are located directly above each tray 8. They are made of light-transmitting polymer material with microstructure texture on the lower surface, which diffuses the light emitted by the LED aperture softly and evenly onto the entire surface of the culture container, significantly improving the uniformity of light and the efficiency of light energy utilization. When the user needs to operate or maintain, the mounting frame 3 can be pulled along the front and back direction by the built-in handle 5 on the front side of the mounting frame 3, so that it can slide out smoothly in the sliding groove 4 through the sliding parts. The rotating mechanism can be removed as a whole without disassembling the motor 6, which is convenient for picking up and putting down the culture container or cleaning and maintenance. The entire working process effectively improves the morphological uniformity, stem and leaf uprightness and physiological health of the cabbage seedlings through the synergistic effect of rotation and beam-splitting illumination, shortens the culture cycle, reduces abnormal phenomena such as vitrification, and takes into account both energy saving and tissue culture quality.

[0025] It should be noted that the control method of this utility model is controlled by a controller. The control circuit of the controller can be implemented by those skilled in the art through simple programming. The power supply is also common knowledge in the field. Furthermore, this utility model is mainly used to protect mechanical devices, so the control method and circuit connection will not be explained in detail here.

[0026] The above description is merely a preferred embodiment of this utility model and is not intended to limit this utility model. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model. It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0027] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A Chinese cabbage tissue culture device with spectrophotometric function, comprising an incubator (1); characterized in that: It also includes a door (2) that is rotatably installed on the front side of the incubator (1). Two sliding grooves (4) are opened at the bottom of the incubator (1). Sliding parts are slidably connected in the sliding grooves (4). A mounting frame (3) is fixed on both sliding parts. A motor (6) is installed inside the mounting frame (3). A vertically arranged rotating rod (7) is connected to the output end of the motor (6). Three horizontally arranged trays (8) are fixed on the outside of the rotating rod (7). Multiple slots for placing culture containers are opened on the surface of the trays (8). Three light source diffuser plates (9) are also fixed on the outside of the rotating rod (7). The three light source diffuser plates (9) are located directly above the corresponding trays (8). The top of the incubator (1) is provided with a sliding groove (10), and a slider (11) is slidably connected in the sliding groove (10). The lower end of the slider (11) is connected to the upper end of the rotating rod (7) through a bearing. Three LED rings (12) are installed on the inner side wall of the incubator (1), and three trays (8) correspond one-to-one with the three LED rings (12). LED rings (13) are installed on the inner surface of the door (2). The LED rings (12) and LED rings (13) together form a continuous 360° beam-splitting illumination structure around the trays (8).

2. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: Both LED aperture one (12) and LED aperture two (13) are composed of red, blue, far-red and white LED chips, and the light intensity of each LED band can be adjusted independently to achieve dynamic spectral control for different tissue culture stages of Chinese cabbage.

3. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: The light source diffuser plate (9) is made of a light-transmitting polymer material, and its lower surface is provided with a microstructure texture, which is used to evenly diffuse the light emitted by the LED aperture onto the entire surface of the culture container on the corresponding tray (8).

4. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: The motor (6) is a low-speed adjustable stepper motor, which drives the rotating rod (7) to rotate the tray (8) at a speed of 0.01 to 0.5 revolutions per minute, thereby eliminating the phenomenon of phototropism caused by unidirectional light on plants.

5. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: The mounting frame (3) is pulled and moved in the front and back direction by the cooperation of the sliding part and the sliding groove (4). The mounting frame (3) is provided with a built-in handle (5) on the front side, which is used to pull out the rotating mechanism as a whole for operation and maintenance without disassembling the motor (6).

6. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: The sliding block (11) in the sliding groove (10) and the bearing connection structure at the upper end of the rotating rod (7) can provide vertical support and allow free rotation during rotation, ensuring that the three-layer tray (8) remains coaxial and stable without shaking during long-term operation.

7. The cabbage tissue culture device with spectrophotometric function according to claim 1, characterized in that: After the door (2) is closed, it forms a sealed cavity with the incubator (1). LED aperture one (12) and LED aperture two (13) are connected when the door (2) is closed, together forming a complete ring light source.

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