A stem cell classification storage device

Abstract

This utility model relates to a stem cell classification and storage device, belonging to the field of stem cell storage technology. It includes a storage box with a flip-top design on the top, a liquid nitrogen tank located below the storage box, and a gas outlet mechanism. The gas outlet mechanism is equipped with a set of nozzles specifically designed for liquid nitrogen, which can reciprocate along the bottom of the storage box. This design allows liquid nitrogen to be sprayed evenly throughout the box, effectively avoiding localized temperature differences and ensuring a consistent temperature across all areas. The liquid nitrogen tank is fixed to the bottom of the storage box, facilitating the movement of the entire device. The tank is securely positioned using a locking structure; simply rotating a knob disengages the insert from the slot, quickly releasing the fixation. This design ensures the stability of the liquid nitrogen tank during use and significantly simplifies the subsequent tank replacement process, requiring no complex tools or specialized operations, effectively improving the convenience of equipment maintenance and use.

Description

Technical Field

[0001] This invention belongs to the field of stem cell storage technology, specifically relating to a stem cell classification and storage device. Background Technology

[0002] Stem cell storage involves isolating and culturing stem cells from different human tissues, then testing and identifying them, and finally freezing them at a deep cryogenic temperature of -196°C. This allows the stem cells to be revived and reinfused into patients when needed in clinical practice to treat diseases. Stem cells can be divided into embryonic stem cells and adult stem cells according to their developmental stage, and their storage requires the use of specialized storage devices.

[0003] For example, in the prior art, Chinese patent with publication number CN219970396U discloses a stem cell classification and storage device. This prior art can further divide the four storage spaces of the partition by attaching stem cell labels to the lifting blocks on the multiple reagent tube classification and lifting mechanisms, thereby enabling more precise classification of reagent tubes containing stem cells.

[0004] However, some shortcomings still exist: Because the liquid nitrogen delivery pipe is directly connected to the container, the liquid cannot reach all parts of the container evenly after entering it, resulting in uneven temperature distribution in different parts of the container and affecting the storage effect.

[0005] Therefore, those skilled in the art have proposed a stem cell classification and storage device. Utility Model Content

[0006] The purpose of this invention is to provide a stem cell classification and storage device that can solve the problems mentioned in the background art.

[0007] The specific technical solution adopted in this utility model is as follows: A stem cell classification and storage device includes a storage box with a flip-top on the upper side, a liquid nitrogen tank below the storage box, and a gas outlet mechanism. The gas outlet mechanism includes a set of sliding rods symmetrically installed between the inner walls of the storage box and near the bottom. A slider is slidably installed on the sliding rod. A horizontal tube is installed between the two sliders. A set of evenly distributed and upward-facing nozzles are installed on the side of the horizontal tube.

[0008] The liquid nitrogen tank has an output end detachably connected to an input pipe extending into the storage tank. A telescopic serpentine tube is connected between the end of the input pipe and the input end of the horizontal tube. A solenoid valve is installed on the input pipe. The gas outlet mechanism also includes a drive assembly installed on the side of the storage tank to drive the horizontal tube to move.

[0009] The present invention is further configured such that: a partition is installed at the center of the storage box to evenly divide its interior into two chambers, and multiple sets of evenly distributed fixing frames are installed in each chamber.

[0010] The present invention is further configured such that: each set of fixing frames is distributed vertically, and the upper fixing frame has multiple evenly distributed circular holes, and the upper side of the lower fixing frame has a groove directly below each circular hole, and test tubes are inserted into the circular holes and the grooves below them.

[0011] The present invention is further configured such that: a fixing seat is installed at the center of the lower side of the storage box, and a set of slots are symmetrically opened on the lower side of the fixing seat.

[0012] The present invention is further configured such that: a card component is provided in the card slot, the lower end of which is connected to the liquid nitrogen tank, and a slot is provided on the side of the card component.

[0013] The present invention is further configured such that: a cavity is provided on one side of each slot inside the fixing base, an insert extending into the adjacent slot is slidably disposed in the cavity, and a spring is installed on one side of the insert in the cavity.

[0014] The present invention is further configured such that: a central groove is provided inside the fixed base between two cavities, a winding roller is rotatably installed in the central groove, and a steel wire rope with its end extending into the central groove and connected to the winding roller is installed at the center of the side of the plug-in.

[0015] The technical effects achieved by this utility model are as follows: This utility model discloses a stem cell classification and storage device. A set of nozzles specifically designed for liquid nitrogen is incorporated into the gas outlet mechanism. These nozzles reciprocate along the bottom of the storage chamber, allowing liquid nitrogen to be sprayed evenly throughout the chamber. This effectively avoids localized temperature differences and ensures that the temperature remains consistent across all areas of the chamber. This temperature uniformity not only significantly reduces the impact of localized temperature variations on stem cells but also further optimizes the stability of the storage environment, significantly improving the overall storage effect. This provides a more reliable guarantee for stem cell storage, making the storage process safer and more practical.

[0016] This utility model discloses a stem cell classification and storage device. The liquid nitrogen tank is installed in the storage box with a bottom-fixed method, which facilitates the movement of the entire device. The tank is stably positioned by a snap-fit ​​structure. Simply rotating the knob can disengage the plug from the slot and quickly release the fixed state. This design not only ensures the installation stability of the liquid nitrogen tank during use, but also greatly simplifies the subsequent tank replacement process. No complicated tools or professional operation are required, which effectively improves the convenience of equipment maintenance and use, saves users operation time, and further enhances the practicality and flexibility of the device. Attached Figure Description

[0017] Figure 1This is a schematic diagram of a stem cell classification and storage device.

[0018] Figure 2 A side view of a stem cell classification and storage device.

[0019] Figure 3 This is a schematic diagram of the main structure of a stem cell classification and storage device.

[0020] Figure 4 This is a schematic diagram of the test tube component structure of a stem cell classification and storage device.

[0021] Figure 5 This is a schematic diagram of the air outlet mechanism of a stem cell classification and storage device.

[0022] Figure 6 This is a schematic diagram of the snap-fit ​​structure of a stem cell classification and storage device.

[0023] Figure 7 A cross-sectional view of the mounting base of a stem cell classification and storage device.

[0024] In the diagram: 1. Storage tank; 2. Gas outlet mechanism; 201. Slide bar; 202. Slider; 203. Horizontal tube; 204. Nozzle; 205. Input tube; 206. Screw; 207. Motor; 208. Threaded seat; 3. Liquid nitrogen tank; 4. Solenoid valve; 5. Fixing base; 6. Slot; 7. Clip; 8. Slot; 9. Cavity; 10. Insert; 11. Spring; 12. Center groove; 13. Winding roller; 14. Wire rope; 15. Knob; 16. Fixture; 17. Test tube; 18. Partition; 19. Handle. Detailed Implementation

[0025] To make the purpose and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific implementations of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0026] like Figure 1-4As shown, a stem cell classification and storage device includes a storage box 1 with a flip-top top. Inside the storage box 1, a partition 18 is installed in the center, dividing the interior into two chambers. Each chamber is equipped with multiple sets of evenly distributed mounting brackets 16. Each set of mounting brackets 16 is arranged vertically, with multiple evenly distributed round holes on the upper mounting bracket 16 and a groove directly below each round hole on the upper side of the lower mounting bracket 16. Test tubes 17 are inserted into both the round holes and the grooves below them. Stem cells are placed into the test tubes 17, and then the test tubes 17 are inserted from the round holes of the upper mounting bracket 16 until they are placed into the bottom groove. The operation is repeated to place other test tubes 17 containing stem cells into the storage box 1. At the same time, stem cells of the same type are placed in the same chamber to achieve classification. After that, the top cover of the storage box 1 is closed.

[0027] like Figure 1 As shown, a latch is installed between the top cover of storage box 1 and the side of the main body. A handle 19 is installed at the center of the upper side of the top cover of storage box 1. The top cover is connected to the main body of storage box 1 by the latch, and then the user can move the device by carrying it through the handle 19.

[0028] like Figure 2 and Figure 5 As shown, a liquid nitrogen tank 3 is located below the storage tank 1, and an exhaust mechanism 2 is also provided. The exhaust mechanism 2 includes a set of slide rods 201 symmetrically installed between the inner walls of the storage tank 1 and near the bottom. Sliding blocks 202 are slidably mounted on the slide rods 201. A horizontal tube 203 is installed between the two sliding blocks 202. A set of evenly distributed and upward-facing nozzles 204 are installed on the side of the horizontal tube 203. The output end of the liquid nitrogen tank 3 is detachably connected to an input pipe 205 (using a dedicated liquid nitrogen delivery pipe) extending into the storage tank 1. The end of the input pipe 205 is connected to the input end of the horizontal tube 203. The connecting hose (using liquid nitrogen vacuum hose with metal corrugated pipe) is equipped with a solenoid valve 4 on the input pipe 205. The gas outlet mechanism 2 also includes a drive assembly installed on the side of the storage tank 1 to drive the horizontal pipe 203 to move. When the solenoid valve 4 and motor 207 are opened, the liquid nitrogen in the liquid nitrogen tank 3 is sent into the horizontal pipe 203 through the input pipe 205 and sprayed into the storage tank 1 by each nozzle 204 to reduce the temperature inside the storage tank 1 and maintain the low temperature environment inside the storage tank 1. At the same time, the drive assembly drives the nozzles 204 to reciprocate at a uniform speed at the bottom of the storage tank 1 to uniformly cool the storage tank 1.

[0029] like Figure 5As shown, the drive assembly includes a screw 206 rotatably mounted on the side of the storage box 1 and at the same height as the slider 202. A motor 207 is mounted on the side of the storage box 1, with its drive end fixedly connected to the end of the screw 206. A threaded seat 208 is threadedly connected to the side of the screw 206 and slidably connected to the storage box 1. A magnetic coupler is provided between the threaded seat 208 and the adjacent slider 202. The running motor 207 drives the screw 206 to rotate, and the rotating screw 206 drives the screw 206 to move back and forth on it. The threaded seat 208 drives the connected slider 202 to move back and forth through the magnetic coupler.

[0030] A temperature sensor is installed inside the storage tank 1, and a controller is installed on the outside of the storage tank 1. The temperature sensor monitors the temperature inside the storage tank 1. When the predetermined temperature is reached, the controller controls the solenoid valve 4 and the motor 207 to close, stopping the delivery of liquid nitrogen. When the temperature is higher than the predetermined temperature, the controller controls the solenoid valve 4 to open and the motor 207 to run to continue cooling.

[0031] like Figure 2 , Figure 6 and Figure 7 As shown, a fixed base 5 is installed at the center of the lower side of the storage tank 1. A set of slots 6 are symmetrically opened on the lower side of the fixed base 5. A clip 7 is installed in the slot 6 and its lower end is connected to the liquid nitrogen tank 3. A slot 8 is opened on the side of the clip 7. A cavity 9 is opened on one side of each slot 6 inside the fixed base 5. A plug 10 extending into the adjacent slot 8 is slidably installed in the cavity 9. A spring 11 is installed on one side of the plug 10 in the cavity 9. A central groove 12 is opened between the two cavities 9 inside the fixed base 5. A winding roller 13 is rotatably installed in the central groove 12. An end extending into the central groove 12 and connecting with the winding roller 13 is installed at the center of the side of the plug 10. The steel wire rope 14 connected to the 3rd winding roller 13 extends to the outside of the fixed base 5 and is equipped with a knob 15. Rotating the knob 15 causes the winding roller 13 to rotate and wind the two steel wire ropes 14, pulling the two plugs 10 closer to each other. While compressing the spring 11, the plugs 10 are removed from the slot 8 and enter the cavity 9, releasing the fixing of the clip 7 on the liquid nitrogen tank 3. The connecting clip 7 of the liquid nitrogen tank 3 is removed, and the two clips 7 of the new liquid nitrogen tank 3 are fully inserted into the slot 6. The knob 15 is released to allow the two springs 11 to rebound and drive the two plugs 10 to return to their original positions and be inserted into the aligned slot 8, thus re-fixing the liquid nitrogen tank 3 on the fixed base 5.

[0032] The working principle of this utility model: Place the stem cells into test tube 17, then insert test tube 17 into the round hole of the upper fixing frame 16 until it is placed into the bottom groove. Repeat the above operation to place other test tubes 17 containing stem cells into storage box 1. At the same time, place stem cells of the same type in the same chamber to achieve classification. Then close the top cover of storage box 1, lock the buckle, and the process is complete.

[0033] First, power is supplied to the internal electrical components of the device, and the solenoid valve 4 and motor 207 are opened. When the solenoid valve 4 is open, liquid nitrogen in the liquid nitrogen tank 3 is sent into the horizontal tube 203 through the input pipe 205, and then sprayed into the storage tank 1 through each nozzle 204 to reduce the temperature inside the storage tank 1 and maintain a low-temperature environment inside the storage tank 1. At the same time, the running motor 207 drives the screw 206 to rotate. The rotating screw 206 drives the threaded seat 208 to move back and forth on it. The moving threaded seat 208 drives the connected slider 202 to move through the magnetic coupler, thereby driving the horizontal tube 203 to move. This allows the nozzles 204 to move back and forth at a uniform speed at the bottom of the storage tank 1, uniformly cooling the storage tank 1. At the same time, the temperature sensor monitors the temperature inside the storage tank 1. After the predetermined temperature is reached, the controller controls the solenoid valve 4 and motor 207 to close, stopping the delivery of liquid nitrogen.

[0034] When the liquid nitrogen in the liquid nitrogen tank 3 is used up, first disconnect the end of the input pipe 205 from the output end of the liquid nitrogen tank 3. Then, rotate the knob 15 to drive the winding roller 13 to rotate and wind the two steel wire ropes 14, pulling the two plugs 10 closer to each other. While compressing the spring 11, the plugs 10 are removed from the slot 8 and enter the cavity 9. Release the fixing of the clip 7 on the liquid nitrogen tank 3, remove the connecting clip 7 of the liquid nitrogen tank 3, and fully insert the two clips 7 of the new liquid nitrogen tank 3 into the slot 6. Release the knob 15 to allow the two springs 11 to rebound and drive the two plugs 10 to return to their original positions and be inserted into the aligned slot 8. Re-fix the liquid nitrogen tank 3 on the fixing base 5, and then connect the input pipe 205 to the output end of the liquid nitrogen tank 3.

[0035] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the art.

Claims

1. A stem cell classification and storage device, comprising a storage box (1) with a flip-top top, and a liquid nitrogen tank (3) disposed below the storage box (1), characterized in that: An air outlet mechanism (2) is also provided. The air outlet mechanism (2) includes a set of slide rods (201) symmetrically installed between the inner walls of the storage box (1) and near the bottom. A slider (202) is slidably arranged on the slide rod (201). A horizontal tube (203) is installed between the two sliders (202). A set of evenly distributed and upward-facing nozzles (204) are installed on the side of the horizontal tube (203). The output end of the liquid nitrogen tank (3) is detachably connected to an input pipe (205) extending into the storage tank (1). A telescopic serpentine tube is connected between the end of the input pipe (205) and the input end of the horizontal pipe (203). A solenoid valve (4) is provided on the input pipe (205). The gas outlet mechanism (2) also includes a drive assembly installed on the side of the storage tank (1) to drive the horizontal pipe (203) to move.

2. The stem cell classification and storage device according to claim 1, characterized in that, The storage box (1) has a partition (18) installed in the center of its interior, which divides its interior into two chambers. Each chamber is equipped with multiple sets of evenly distributed fixing frames (16).

3. A stem cell classification and storage device according to claim 2, characterized in that, Each set of fixing frames (16) is distributed vertically, and the upper fixing frame (16) has multiple evenly distributed round holes. The lower fixing frame (16) has a groove on the upper side directly below each round hole. Test tubes (17) are inserted into the round holes and the grooves below them.

4. A stem cell classification and storage device according to claim 3, characterized in that, A fixing seat (5) is installed at the center of the lower side of the storage box (1), and a set of slots (6) are symmetrically opened on the lower side of the fixing seat (5).

5. A stem cell classification and storage device according to claim 4, characterized in that, The slot (6) is provided with a card (7) whose lower end is connected to the liquid nitrogen tank (3), and the side of the card (7) is provided with a slot (8).

6. A stem cell classification and storage device according to claim 5, characterized in that, The fixed base (5) has a cavity (9) on one side of each slot (6). A plug (10) extending into the adjacent slot (8) is slidably disposed in the cavity (9). A spring (11) is installed on one side of the plug (10) in the cavity (9).

7. A stem cell classification and storage device according to claim 6, characterized in that, The fixed base (5) has a central groove (12) between two cavities (9). A winding roller (13) is rotatably installed in the central groove (12). A wire rope (14) with its end extending into the central groove (12) and connected to the winding roller (13) is installed on the center side of the plug (10).

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