Simple and portable device for heart perfusion of rats and mice

Abstract

The application relates to a simple and portable mouse heart perfusion device, and relates to the technical field of tissue perfusion. The simple and portable mouse heart perfusion device comprises a perfusion device main body, the inside of the perfusion device main body is divided into a first chamber and a second chamber through a partition block, a seal device strip capable of moving reversely is arranged in the two chambers, a control mechanism for controlling the sliding of the two groups of seal strips, and the control mechanism comprises: a gear rotatably arranged on the partition block; a first rack and a second rack, which are respectively engaged with the gear and reversely move under the drive of the gear; and a driving assembly for driving the gear to rotate. The perfusion liquid in the first chamber is injected into the left ventricle, and the second chamber recycles the waste liquid discharged from the right ventricle; the driving assembly drives the gear to rotate and reversely moves the first rack and the second rack, and then reversely moves the seal device strips in the first chamber and the second chamber, so that the perfusion and recycling are synchronously conducted, and the technical defect of the waste liquid treatment deficiency of the traditional heart perfusion device is solved.

Description

Technical Field

[0001] This application relates to the technical field of tissue perfusion, and in particular to a simple and portable perfusion device for rat and mouse hearts. Background Technology

[0002] Tissue perfusion is an important experimental technique in medicine and scientific research, primarily used in pharmacological and physiological studies. Its core principle is to replace the blood within the blood vessels of a tissue or organ with a perfusion fluid, thereby eliminating interference from blood components in subsequent experiments. For example, in isolated brain tissue studies, specific culture media are delivered to the organ via cardiac perfusion to maintain tissue physiological activity and prevent residual blood from affecting test results. This technique is widely used in exploring the physiological mechanisms of organs such as blood vessels, liver, lungs, and intestines, as well as in evaluating drug effects.

[0003] In existing technologies, cardiac perfusion typically involves injecting saline solution using a simple device. A conventional perfusion device consists of a support, dissecting plate, water bath pump, and other components, and perfusion is performed manually. The specific procedure involves inserting a perfusion needle into the left ventricle of the animal's heart, injecting saline solution to flush the blood vessels, and simultaneously manually fixing the heart in place and collecting any overflowing perfusion fluid. While such devices can fulfill basic perfusion needs, they are complex in structure, bulky, and require manual operation based on the experience of the laboratory personnel.

[0004] However, existing technologies have significant drawbacks: due to the lack of a sealed recycling design during the perfusion process, the residual waste liquid containing harmful substances such as paraformaldehyde cannot be effectively treated, which not only pollutes the experimental environment but also poses a potential threat to the health of experimental personnel. Utility Model Content

[0005] To address the technical shortcomings of traditional cardiac perfusion devices in terms of waste fluid treatment, this application provides a simple and portable cardiac perfusion device for rats and mice.

[0006] This application provides a simple and portable heart perfusion device for rats and mice, which adopts the following technical solution: A simple, portable cardiac perfusion device for rats and mice includes a main body. The main body is internally divided into a first chamber and a second chamber arranged in parallel by a partition block. The first chamber is connected to the perfusion port of the left ventricle via a first flexible tube, and the second chamber is connected to the return port of the right ventricle via a second flexible tube. Each of the first and second chambers is equipped with a reversibly movable sealing strip. The partition block is equipped with a control mechanism for controlling the sliding of the two sealing strips. The control mechanism includes: Gear, the gear being rotatably mounted on the isolation block; The first rack and the second rack are respectively meshed with the gear and connected to the sealing device strips of the first chamber and the second chamber. When the gear rotates, it drives the first rack and the second rack to move in opposite directions, so as to realize the synchronization of irrigation and recovery. A drive assembly is disposed on the body of the irrigation device and is used to drive the gear to rotate.

[0007] By adopting the above technical solution, the perfusion fluid is injected into the left ventricle through the first chamber and the first tubing, and the waste fluid is simultaneously recovered from the right ventricle through the second chamber and the second tubing; the drive component drives the gear to rotate and drives the first rack and the second rack to move in opposite directions, thereby driving the sealing device strips in the first chamber and the second chamber to move in opposite directions, ensuring that perfusion and recovery are carried out simultaneously, thus solving the technical defect of the lack of waste fluid treatment in traditional cardiac perfusion devices.

[0008] Furthermore, an irrigation chamber is formed between the sealing device strip in the first chamber and the connection of the first hose, and a recovery chamber is formed between the sealing device strip in the second chamber and the connection of the second hose. The isolation block is symmetrically provided with sliding grooves on opposite sides to facilitate the sliding of the first rack and the second rack. A sealing layer is provided on the opposite side of the first rack and the second rack. The sealing layer slides with the first rack and slides to seal the sliding groove in the irrigation chamber.

[0009] By adopting the above technical solution, the independent design of the irrigation chamber and the recovery chamber, combined with the sealing layer to slide and seal the sliding groove, a dual discharge mechanism is finally formed, which effectively prevents liquid from seeping into the non-working area, significantly reduces pollution, and improves the sealing performance of the device.

[0010] Furthermore, the main body of the irrigation device is provided with a protective cover, and a guide groove is provided inside the protective cover to guide the sliding of the first rack and the second rack.

[0011] By adopting the above technical solutions, the protective cover and guide groove enhance the stability of the rack sliding, prevent external interference from causing the mechanism to jam or deviate, and protect the precision transmission components, thus extending the service life of the device.

[0012] Furthermore, the side of the first chamber away from the irrigation chamber and the side of the second chamber away from the recovery chamber are internally connected to form a sealed safety chamber. When the two sets of sealing device strips slide synchronously in opposite directions, the air pressure value in the safety chamber remains unchanged.

[0013] By adopting the above technical solution, the safety chamber automatically corrects the difference in the moving speed of the sealing strip through air pressure balance, ensuring the synchronization of irrigation and recovery, avoiding pressure imbalance caused by mechanical errors or external interference, and improving operational reliability.

[0014] Furthermore, the main body of the irrigation device is made of transparent material, and volume scale lines are provided on the outer side walls of both the first chamber and the second chamber.

[0015] By adopting the above technical solution, the transparent material and volume scale lines allow experimenters to intuitively monitor the perfusion fluid flow rate and recovery status, facilitating real-time parameter adjustment and improving experimental accuracy and operational convenience.

[0016] Furthermore, both the first and second tubing ends are provided with perfusion fixation needles, and the perfusion fixation needles are provided with fixation components to facilitate fixing and snapping the perfusion fixation needles into the heart.

[0017] By adopting the above technical solutions, the perfusion fixation needle and fixation components simplify the cardiac positioning process, avoid needle displacement or slippage caused by traditional manual fixation, and improve operational safety and stability.

[0018] Furthermore, the perfusion fixing needle has an annular groove on the side near the needle tip, and the fixing assembly includes: Support rods, multiple sets of the support rods are arranged in a circumferential array in an annular groove, and the support rods are hinged to the irrigation fixing needle on the side near the needle tip; A sliding ring, wherein the sliding ring is disposed in an annular groove and slides along the axis of the irrigation fixing needle; A drive rod, the two ends of which are respectively hinged to a sliding ring and a support rod. When the sliding ring slides, it pushes the support rod to unfold or retract through the drive rod. A compression spring is fitted inside an annular groove. The compression spring pushes the sliding ring to slide towards the needle tip side and causes the support rod to unfold. The support cloth is set on the irrigation fixing needle and wraps around multiple sets of support rods in the annular groove. The support cloth is stretchable and deformable and expands with the support rods to increase the end diameter to clamp the heart. By adopting the above technical solution, the umbrella-shaped support rod and the elastic support cloth are unfolded by the spring and clamp the heart tissue, increasing the contact area and preventing the needle from falling off due to pressure fluctuations or animal tremors; the support cloth wrapping reduces tissue damage and achieves non-invasive fixation.

[0019] Furthermore, the irrigation fixing needle is provided with an unlocking component, which includes a connecting rod and an unlocking ring. Multiple sets of the connecting rods are connected to the unlocking ring, and the unlocking ring is slidably disposed on the side of the irrigation fixing needle away from the needle tip. The unlocking ring is driven to reset by the sliding ring through multiple sets of connecting rods.

[0020] By adopting the above technical solution, pulling the unlocking ring to slide and reset the sliding ring allows multiple sets of support rods to fully retract into the annular groove, thereby facilitating the insertion or removal of the perfusion fixation needle into or from the heart chamber, simplifying the needle insertion and removal steps, avoiding mechanical damage to the heart from secondary operations, and improving experimental efficiency and safety.

[0021] In summary, this application includes at least one of the following beneficial technical effects: 1. The perfusion fluid is injected into the left ventricle through the first chamber and the first tubing, and the waste fluid is simultaneously recovered from the right ventricle through the second chamber and the second tubing; the drive assembly drives the gear to rotate and drives the first rack and the second rack to move in opposite directions, thereby driving the sealing device strips in the first chamber and the second chamber to move in opposite directions, ensuring that perfusion and recovery are carried out synchronously. At the same time, the sealing layer and the sliding groove slide and seal together to prevent the liquid in the perfusion chamber and the recovery chamber from overflowing, thus solving the technical defect of the lack of waste fluid treatment in traditional cardiac perfusion devices.

[0022] 2. The perfusion fixation needle is secured to the heart tissue after unfolding through the umbrella-shaped support rod and the elastic support cloth, reducing the probability of accidental dislodgement of the perfusion fixation needle.

[0023] 3. The safety chamber connects the non-working sides of the two chambers and automatically corrects the difference in the moving speed of the sealing strip through an air pressure balance mechanism to ensure synchronization. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the simple portable rat and mouse heart perfusion device of this application; Figure 2 yes Figure 1 A schematic diagram of a half-section structure; Figure 3 yes Figure 2 A cross-sectional schematic diagram of AA in the middle; Figure 4 yes Figure 2 Enlarged schematic diagram of section B.

[0025] Reference numerals: 1. Main body of the irrigation device; 11. First chamber; 111. Irrigation chamber; 12. Second chamber; 121. Recovery chamber; 13. First hose; 14. Second hose; 15. Isolation block; 151. Sliding groove; 16. Safety chamber; 2. Sealing device strip; 21. First sealing strip; 22. Second sealing strip; 23. Sealing layer; 3. Control mechanism; 31. Gear; 32. First rack; 33. Second rack; 4. Protective cover; 41. Guide groove; 5. Irrigation fixing pin; 51. Annular groove; 6. Fixing assembly; 61. Support rod; 62. Sliding ring; 63. Drive rod; 64. Compression spring; 65. Support cloth; 7. Unlocking component; 71. Connecting rod; 72. Unlocking ring. Detailed Implementation

[0026] The following is in conjunction with the appendix Figures 1-4 This application will be described in further detail.

[0027] This application discloses a simple and portable cardiac perfusion device for rats and mice.

[0028] Reference Figure 1 and Figure 2 A simple and portable perfusion device for rat and mouse hearts includes a main body 1. The main body 1 is divided into a first chamber 11 and a second chamber 12 arranged in parallel by an isolation block 15. The first chamber 11 is connected to the perfusion port of the left ventricle through a first hose 13, and the second chamber 12 is connected to the return port of the right ventricle through a second hose 14. The first chamber 11 and the second chamber 12 are provided with sealing strips 2 that can move in opposite directions. The isolation block 15 is provided with a control mechanism 3 for controlling the sliding of the two sets of sealing strips.

[0029] Reference Figure 2 The main body 1 of the irrigation device is a hollow box structure. The internal structure of the main body 1 is divided into a first chamber 11 and a second chamber 12 by an isolation block 15. The internal structures of the first chamber 11 and the second chamber 12 are symmetrical. The sealing device strip 2 is divided into a first sealing strip 21 and a second sealing strip 22. The first sealing strip 21 is slidably installed in the first chamber 11 and slides in sealing contact with the side wall of the first chamber 11, dividing the first chamber 11 into two mutually isolated and sealed cavities. The second sealing strip 22 is slidably installed in the second chamber 12 and slides in sealing contact with the side wall of the second chamber 12, dividing the second chamber 12 into two mutually isolated and sealed cavities.

[0030] Reference Figure 2The control mechanism 3 includes a gear 31, a first rack 32, a second rack 33, and a drive assembly. The gear 31 is rotatably mounted on the isolation block 15. The isolation block 15 has two sets of sliding grooves 151 symmetrically opened on opposite side walls. The first rack 32 is slidably mounted in the sliding groove 151 near the first chamber 11, and the first rack 32 meshes with one side of the gear 31. The first sealing strip 21 is fixedly connected to the bottom of the first rack 32 and slides with the first rack 32 in the first chamber 11. The second rack 33 is slidably mounted in the sliding groove 151 near the second chamber 12, and the second rack 33 meshes with one side of the gear 31. The second sealing strip 21 is fixedly connected to the bottom of the first rack 32 and slides with the first rack 32 in the first chamber 11. 2 is fixedly connected to the bottom of the second rack 33, and the second sealing strip 22 slides in the second chamber 12 along with the second rack 33; when the gear 31 rotates, it simultaneously drives the first rack 32 and the second rack 33 to rotate in opposite directions, thereby driving the first sealing strip 21 and the second sealing strip 22 to slide and seal in the first chamber 11 and the second chamber 12 respectively; the drive assembly is set on the main body 1 of the irrigation device, and the drive assembly is used to drive the gear 31 to rotate; the drive assembly of this embodiment includes a drive motor, a controller and a drive power supply, the drive power supply is used to supply power to the drive motor, the controller is used to control the start and stop and the rotation speed of the drive motor, and the drive motor is used to drive the gear 31 to rotate.

[0031] Reference Figure 2 and Figure 3 An irrigation chamber 111 is formed between the top of the first sealing strip 21 and the connection of the first hose 13 in the first chamber 11. A recovery chamber 121 is formed between the top of the second sealing strip 22 and the connection of the second hose 14 in the second chamber 12. A sealing layer 23 is fixedly installed on the opposite side of the first rack 32 and the second rack 33. When the sealing layer 23 slides with the first rack 32, the sealing layer 23 slides and seals the sliding groove 151 in the irrigation chamber 111, thereby preventing the liquid in the irrigation chamber 111 from entering the sliding groove 151. At the same time, when the sealing layer 23 slides with the second rack 33, the sealing layer 23 seals the sliding groove 151 in the recovery chamber 121.

[0032] Reference Figure 2 A protective cover 4 is fixedly installed on the main body 1 of the irrigation device. The protective cover 4 has a guide groove 41 inside to guide the sliding of the first rack 32 and the second rack 33. The protective cover 4 improves the stability of the sliding of the first rack 32 and the second rack 33, and at the same time reduces the probability of damage to the first rack 32 and the second rack 33 by the external environment.

[0033] Reference Figure 2The sealed cavity on the side of the first chamber 11 away from the irrigation cavity 111 is connected to the sealed cavity on the side of the second chamber 12 away from the recovery cavity 121, forming a relatively sealed safety cavity 16. When the first sealing strip 21 and the second sealing strip 22 slide synchronously in opposite directions, the total volume of the safety cavity 16 remains unchanged because the sliding speed of the first sealing strip 21 is the same, and the air pressure value in the safety cavity 16 remains unchanged. However, when one of the first sealing strips 21 or the second sealing strip 22 is abnormally fast or slow, the air pressure value of the safety cavity 16 changes due to the increase or decrease in the total volume. Finally, under the action of air pressure, the sliding speed of the first sealing strip 21 and the second sealing strip 22 tends to be equal.

[0034] Reference Figure 1 The main body 1 of the irrigation device is made of transparent material. Volume scale lines are provided on the outer walls of the first chamber 11 and the second chamber 12. The volume of liquid in the irrigation chamber and the recovery chamber 121 can be obtained intuitively through the volume scale lines.

[0035] Reference Figure 4 Both the first tubing 13 and the second tubing 14 are equipped with perfusion fixation needles 5 at their ends. Each perfusion fixation needle 5 has a fixation component 6 for securing it within the heart. The fixation component 6 includes a support rod 61, a sliding ring 62, a drive rod 63, a compression spring 64, and a support cloth 65. An annular groove 51 is formed on the side of the perfusion fixation needle 5 near the needle tip. The support rod 61 is hinged to the perfusion fixation needle 5 near the needle tip. When the support rod 61 is parallel to the perfusion fixation needle 5, it is fully retracted into the annular groove 51. Multiple sets of support rods 61 are arranged in a circumferential array within the annular groove 51. The sliding ring 62 is fitted within the annular groove 51 and can slide along the axis of the perfusion fixation needle 5. The two ends of the drive rod 63 are respectively connected to the sliding ring 62. The support rod 61 is hinged to the drive rod 63, which corresponds to the support rod 61. When the sliding ring 62 slides, it pushes the support rod 61 to unfold or retract through the drive rod 63. The compression spring 64 is sleeved in the annular groove. The compression spring 64 pushes the sliding ring 62 to slide towards the needle tip, thereby unfolding the support rod 61. The support cloth 65 is installed on the perfusion fixation needle 5. The support cloth 65 is used to wrap the multiple sets of support rods 61 in the annular groove 51. The support cloth 65 is extensible and deformable. The support cloth 65 unfolds with the support rods 61 and increases the end face diameter. When the multiple sets of support rods 61 are fully unfolded, the diameter on the side away from the needle tip is larger than the diameter on the side closer to the needle tip, and the diameter on the side away from the needle tip is much larger than the outer diameter of the perfusion fixation needle 5, thereby preventing the perfusion fixation needle 5 from sliding out of the heart.

[0036] Reference Figure 4The irrigation fixing needle 5 is provided with an unlocking component 7, which includes a connecting rod 71 and an unlocking ring 72. Multiple sets of connecting rods 71 ​​are slidably installed on the irrigation fixing needle 5. The sliding direction of the connecting rods 71 ​​is parallel to the sliding direction of the sliding ring 62. Multiple sets of connecting rods 71 ​​are arranged in a circumferential array on the sliding ring 62. The unlocking ring 72 is slidably installed on the side of the irrigation fixing needle 5 away from the needle tip. The unlocking ring 72 drives the sliding ring 62 to reset through multiple sets of connecting rods 71, so that multiple sets of support rods 61 retract into the annular groove 51.

[0037] Reference Figure 4 Specifically, before inserting the perfusion fixation needle 5 into the heart, the unlocking ring 72 is pulled to slide and the sliding ring 62 is reset, so that the multiple sets of support rods 61 are completely retracted into the annular groove. Then, the perfusion fixation needle 5 is inserted into the heart chamber. When the perfusion fixation needle 5 is inserted to the specified depth, the unlocking ring 72 is released, so that the sliding ring 62 is pushed to slide closer to the needle tip under the action of the compression spring 64. The support rod 61 is driven to unfold by the drive rod 63. At the same time, the support cloth 65 stretches and deforms and covers the multiple sets of support rods 61. The support rods 61 abut against the side wall of the heart chamber through the support cloth 65, thereby preventing the perfusion fixation needle 5 from sliding out of the heart.

[0038] The working principle of this application embodiment is as follows: The perfusion fluid is injected into the left ventricle through the first chamber 11 and the first tubing 13, while waste fluid is simultaneously recovered from the right ventricle through the second chamber 12 and the second tubing 14. The perfusion fixation needle 5 is secured to the heart tissue after unfolding through the umbrella-shaped support rod 61 and the elastic support cloth 65, reducing the probability of accidental dislodgement of the perfusion fixation needle 5. The drive assembly drives the gear 31 to rotate and drives the first rack 32 and the second rack 33 to move in opposite directions, thereby driving the sealing device strip 2 in the first chamber 11 and the second chamber 12 to move in opposite directions, ensuring that perfusion and recovery are carried out synchronously. At the same time, the sealing layer 23 and the sliding groove 151 slide and seal to prevent the liquid in the perfusion chamber and the recovery chamber 121 from overflowing, solving the technical defect of the lack of waste fluid treatment in traditional cardiac perfusion devices.

[0039] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A simple and portable rat and mouse heart perfusion device, characterized in that: The device includes a main body (1) of an irrigation device. The main body (1) is divided into a first chamber (11) and a second chamber (12) arranged in parallel by an isolation block (15). The first chamber (11) is connected to the infusion port of the left ventricle through a first hose (13), and the second chamber (12) is connected to the return port of the right ventricle through a second hose (14). The first chamber (11) and the second chamber (12) are provided with reversibly movable sealing strips (2). The isolation block (15) is provided with a control mechanism (3) for controlling the sliding of the two sets of sealing strips. The control mechanism (3) includes: Gear (31), which is rotatably mounted on the isolation block (15); The first rack (32) and the second rack (33) mesh with the gear (31) and are connected to the sealing device strip (2) of the first chamber (11) and the second chamber (12). When the gear (31) rotates, it drives the first rack (32) and the second rack (33) to move in opposite directions, so as to realize the synchronization of irrigation and recycling. A drive assembly is disposed on the body (1) of the irrigation device and is used to drive the gear (31) to rotate.

2. The simple portable rat and mouse heart perfusion device according to claim 1, characterized in that: An irrigation chamber (111) is formed between the sealing device strip (2) in the first chamber (11) and the first hose (13). A recovery chamber (121) is formed between the sealing device strip (2) in the second chamber (12) and the second hose (14). The isolation block (15) is symmetrically provided with sliding grooves (151) on both sides to facilitate the sliding of the first rack (32) and the second rack (33). A sealing layer (23) is provided on the opposite side of the first rack (32) and the second rack (33). The sealing layer (23) slides with the first rack (32) and slides to seal the sliding groove (151) in the irrigation chamber (111).

3. A simple portable rat and mouse heart perfusion device according to claim 2, characterized in that: The main body (1) of the irrigation device is provided with a protective cover (4), and a guide groove (41) is provided inside the protective cover (4) to guide the sliding of the first rack (32) and the second rack (33).

4. A simple portable rat and mouse heart perfusion device according to claim 3, characterized in that: The side of the first chamber (11) away from the irrigation chamber (111) is internally connected with the side of the second chamber (12) away from the recovery chamber (121) to form a sealed safety chamber (16). When the two sets of sealing device strips (2) slide in opposite directions synchronously, the air pressure value in the safety chamber (16) remains unchanged.

5. A simple portable rat and mouse heart perfusion device according to claim 1, characterized in that: The main body (1) of the irrigation device is made of transparent material, and volume scale lines are provided on the outer walls of the first chamber (11) and the second chamber (12).

6. A simple portable rat and mouse heart perfusion device according to claim 1, characterized in that: The first tubing (13) and the second tubing (14) are each provided with an irrigation fixation needle (5), and the irrigation fixation needle (5) is provided with a fixation component (6) to facilitate fixing the irrigation fixation needle (5) in the heart.

7. A simple portable rat and mouse heart perfusion device according to claim 6, characterized in that: The perfusion fixation needle (5) has an annular groove (51) on the side near the needle tip, and the fixation component (6) includes: Support rod (61), multiple sets of the support rod (61) are arranged in a circumferential array in an annular groove (51), and the support rod (61) is hinged to the irrigation fixing needle (5) on the side near the needle tip; Sliding ring (62), the sliding ring (62) is provided in the annular groove (51) and slides along the axis of the irrigation fixing needle (5); The drive rod (63) is hinged to the sliding ring (62) and the support rod (61) at both ends. When the sliding ring (62) slides, it pushes the support rod (61) to unfold or retract through the drive rod (63). A compression spring (64) is fitted in an annular groove. The compression spring (64) pushes the sliding ring (62) to slide towards the needle tip and causes the support rod (61) to unfold. Support cloth (65) is placed on the irrigation fixing needle (5) and wraps the multiple sets of support rods (61) in the annular groove (51). The support cloth (65) is stretchable and deformable and expands with the support rods (61) to increase the end diameter to clamp the heart.

8. A simple portable rat and mouse heart perfusion device according to claim 7, characterized in that: The irrigation fixing needle (5) is provided with an unlocking component (7), which includes a connecting rod (71) and an unlocking ring (72). Multiple sets of the connecting rods (71) are connected to the unlocking ring (72). The unlocking ring (72) is slidably disposed on the side of the irrigation fixing needle (5) away from the needle tip. The unlocking ring (72) drives the sliding ring (62) to reset through multiple sets of connecting rods (71).

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