An escherichia coli fermentation broth membrane separation and clarification apparatus

Abstract

The utility model relates to the field of membrane separation technology, and disclose a kind of escherichia coli fermentation broth membrane separation clarification equipment, including bottom plate, the top of the bottom plate is provided with controller, the top of the bottom plate is fixedly installed with support plate symmetrically, the top of the bottom plate is provided with support frame, the support frame is arranged in one side of support plate, stirring assembly is arranged between the support plate.The utility model is through setting filter cartridge two, the bottom of filter cartridge two is clamped with the annular clamping groove one and the annular clamping plate one of support seat, the top is clamped with the annular clamping groove two and the annular clamping plate two of clamping top cover, cooperation motor two drive threaded rod drives clamping top cover to move down and press tightly, form the double fixed structure of bottom support and top clamping;Completely solve the problem that membrane assembly is easily impacted by liquid deviation, shaking in traditional equipment, ensure that fermentation broth flows through the surface of flat plate membrane evenly, no filter dead angle, improve the purity of clarified liquid.

Description

Technical Field

[0001] This utility model relates to the field of membrane separation technology, specifically to a membrane separation and clarification device for Escherichia coli fermentation broth. Background Technology

[0002] Escherichia coli fermentation broth refers to a mixed liquid substance containing Escherichia coli cells, metabolites, incompletely consumed culture medium components, and water obtained after culturing and metabolizing Escherichia coli (a common Gram-negative bacterium) under artificially controlled fermentation conditions (such as suitable temperature, pH value, and nutrient environment).

[0003] In the field of membrane separation and clarification of E. coli fermentation broth, traditional equipment suffers from significant deficiencies in the core separation and pretreatment stages due to structural design flaws, making it difficult to meet the demands of efficient and stable production. Firstly, in traditional equipment, the core components of membrane separation are often installed using simple bolt fixing or direct placement, lacking precise positioning and clamping structures. During the fermentation broth transport process, the impact force generated by the liquid flow easily causes membrane module displacement and shaking, preventing the fermentation broth from flowing evenly across the membrane surface and resulting in localized filtration dead zones. Secondly, the raw material storage tanks in traditional equipment generally lack effective stirring structures. Because the bacterial cells in the E. coli fermentation broth are denser than the liquid, they easily settle and stratify at the bottom of the tank, leading to uneven concentrations of the fermentation broth within the tank. When this uneven fermentation broth is transported to subsequent separation components, the locally high concentrations of bacterial aggregates instantly increase the separation load, not only reducing separation efficiency but also easily clogging the filter pores of the membrane module. Utility Model Content

[0004] The purpose of this invention is to provide a membrane separation and clarification device for Escherichia coli fermentation liquid, which solves the technical problem that the membrane module in traditional equipment is easily affected by liquid impact and displacement due to the simple fixing method, resulting in filtration dead corners. It achieves the purpose of stable fixing of the membrane module and ensuring uniform concentration of fermentation liquid.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a liquid membrane separation and clarification device for Escherichia coli fermentation, comprising a base plate, with a controller mounted on the top of the base plate.

[0006] A support plate is symmetrically fixedly installed on the top, a support frame is provided on the top of the bottom plate, the support frame is located on one side of the support plate, a stirring assembly is provided between the support plates, and a clarification assembly is provided between the support frame and the bottom plate.

[0007] Preferably, the stirring assembly includes: a raw material storage tank, fixedly installed between support plates; a liquid inlet pipe, connected to one side of the raw material storage tank; and a motor, installed on the top of the raw material storage tank.

[0008] Preferably, the output end of the motor is provided with a stirring shaft, the other end of which movably passes through the top of the raw material storage tank and extends into the interior of the raw material storage tank. Stirring rods are equidistantly installed around the bottom of the outer wall of the motor. A liquid outlet pipe is connected to the bottom of the raw material storage tank. A filter cylinder is threadedly connected to the bottom of the raw material storage tank. The liquid outlet pipe extends into the interior of the filter cylinder. A fixing plate is symmetrically fixed to the inner wall of the filter cylinder. A filter basket is provided between the fixing plates. A connecting pipe is connected to the bottom of the filter cylinder. A pressure sensing module is provided at the bottom of the inner wall of the filter cylinder. A centrifugal pump is connected to the other end of the connecting pipe. A connecting pipe is connected to the output end of the centrifugal pump.

[0009] Once the motor drives the stirring shaft and stirring rollers to rotate, it can prevent the fermentation broth cells in the raw material storage tank from settling and stratifying, ensuring the uniform concentration of the fermentation broth and laying the foundation for subsequent separation.

[0010] Preferably, the clarification assembly includes: a filter component disposed between the support frame and the base plate; and a fixing component disposed on one side of the filter component.

[0011] Preferably, the filter component includes: a fixing block 1, which is fixedly installed on the top of the inner wall of the support frame;

[0012] Fixed block two is fixedly installed on the top of the base plate; filter cylinder two is evenly arranged between fixed block one and fixed block two.

[0013] Preferably, the bottom of the fixing block one is provided with a horizontal inlet pipe, one end of which is connected to the other end of the connecting pipe two via a connector. Vertical inlet pipes are equidistantly connected to the bottom of the horizontal inlet pipe. A storage pipe is fixedly installed on the top of the fixing block two, one end of which is connected to a drain pipe. Support seats are equidistantly arranged on the top of the storage pipe, and an annular locking plate is fixedly installed on the top of the support seats. An annular locking groove is formed at the bottom of the filter cylinder two.

[0014] The top of the filter cartridge two is provided with an annular locking groove two. The top of the support base is fixedly installed with an annular locking plate one. The inside of the support base is provided with a connection hole one. The top of the liquid storage tube is provided with connection holes two at equal intervals. The connection holes one and the connection holes two are connected. The annular locking plate one is engaged with the bottom of the filter cartridge two through the annular locking groove one. The bottom end of the filter cartridge two is movably connected through the connection holes one and two. The top of the filter cartridge two is provided with a liquid inlet hole. The bottom end of the liquid inlet vertical pipe is movably connected to the filter cartridge two through the liquid inlet hole. Flat sheet membranes are uniformly arranged inside the filter cartridge two.

[0015] The second filter cartridge is precisely engaged with the first annular locking plate of the support base via the first annular locking groove, which not only achieves stable fixation but also facilitates quick disassembly and maintenance. At the same time, the flat sheet membrane can accurately trap bacteria and impurities, ensuring the purity of the clarified liquid.

[0016] Preferably, the fixing component includes: a fixing vertical plate, which is fixedly installed on one side of the support frame.

[0017] Preferably, the fixed vertical plate has a sliding opening inside, and a second fixed plate is symmetrically fixedly installed on the outer side of the fixed vertical plate. A second motor is provided on the top of the second fixed plate, and a threaded rod is provided at the output end of the second motor. The other end of the threaded rod movably passes through the top and bottom of the second fixed plate and extends to the top of another second fixed plate. A movable rod is threadedly connected to the outer wall of the threaded rod. The other end of the movable rod movably passes through the sliding opening and is fixedly installed with a clamping top cover. There are three sets of clamping top covers, which are fixedly connected by connecting blocks. The clamping top cover is movably sleeved on the outer wall of the liquid inlet vertical pipe. An annular locking plate is fixedly installed at the bottom of the clamping top cover. The annular locking plate is engaged with the top of the filter cylinder through an annular locking groove. The clamping top cover is adapted to the top of the filter cylinder.

[0018] Motor 2 drives the threaded rod to move the movable rod downward, so that the annular locking plate 2 that holds the top cover locks into the top of the filter cylinder 2. Together with the bottom support, it achieves double fixation, avoids the impact of fermentation liquid causing the filter cylinder 2 to shift, and ensures uniform filtration of the flat sheet membrane.

[0019] This invention provides a membrane separation and clarification device for Escherichia coli fermentation liquid. It has the following beneficial effects:

[0020] (1) This utility model provides a second filter cylinder, the bottom of which is connected by an annular locking groove.

[0021] The annular locking plate 1 engages with the support base, and the top engages with the annular locking plate 2 of the clamping top cover through the annular locking groove 2. With the help of the motor 2 driving the threaded rod, the clamping top cover moves down and is pressed tightly, forming a double fixing structure of bottom support and top clamping. This completely solves the problem of membrane modules being easily displaced and shaken by liquid impact in traditional equipment, ensuring that the fermentation broth flows evenly across the surface of the flat membrane without dead corners and improving the purity of the clarified broth.

[0022] (2) This utility model drives the stirring shaft and stirring rod to rotate by a motor, which can stir the E. coli fermentation liquid in the raw material storage tank at low speed, effectively preventing the bacteria from settling and stratifying due to high density, and ensuring uniform concentration of fermentation liquid; when it is subsequently transported to the clarification component, it avoids the increase in separation load caused by excessively high concentration of local bacterial aggregates, reduces the risk of blockage of the separation component, and improves the overall processing efficiency. Attached Figure Description

[0023] Figure 1 is a three-dimensional schematic diagram of the overall structure of this utility model;

[0024] Figure 2 is a cross-sectional view of the stirring assembly of this utility model;

[0025] Figure 3 is a cross-sectional view of the clarification component structure of this utility model;

[0026] Figure 4 is a cross-sectional view of the fixing component structure of this utility model.

[0027] In the diagram: 1. Base plate, 2. Controller, 3. Support plate, 4. Support frame, 5. Stirring assembly, 6. Clarifying assembly;

[0028] 511 Raw material storage tank, 512 Liquid inlet pipe, 513 Motor 1, 514 Stirring shaft, 515 Stirring rod, 516 Filter cylinder 1, 517 Liquid outlet pipe, 518 Fixing plate 1, 519 Filter basket, 5111 Connecting pipe 1, 5112 Centrifugal pump, 5113 Connecting pipe 2, 5114 Pressure sensing module;

[0029] 61 Filter component, 611 Fixing block one, 612 Inlet horizontal pipe, 613 Connector, 614 Inlet vertical pipe, 615 Fixing block two, 616 Storage pipe, 617 Drain pipe, 618 Support base, 619 Annular locking plate one, 6111 Filter cylinder two, 6112 Flat sheet membrane;

[0030] 62 Fixed component, 621 Fixed vertical plate, 622 Fixed plate II, 623 Motor II, 624 Threaded rod, 625 Movable rod, 626 Clamping top cover, 627 Connecting block, 628 Annular locking plate II. Detailed Implementation

[0031] The technology of the present invention will now be described with reference to the accompanying drawings of the embodiments thereof.

[0032] The solution is described clearly and completely. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0033] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0034] Example 1:

[0035] Based on the existing problems of membrane modules in traditional equipment being easily displaced and shaken by liquid impact due to the simple fixing method, resulting in filtration dead zones, the present invention provides a preferred embodiment of an E. coli fermentation liquid membrane separation and clarification device, as shown in Figures 1-4: An E. coli fermentation liquid membrane separation and clarification device includes a base plate 1, a controller 2 mounted on the top of the base plate 1, support plates 3 symmetrically fixedly mounted on the top of the base plate 1, a support frame 4 mounted on the top of the base plate 1, the support frame 4 being located on one side of the support plates 3, a stirring assembly 5 being arranged between the support plates 3, and a clarification assembly 6 being arranged between the support frame 4 and the base plate 1. The stirring assembly 5 includes: a raw material storage tank 511, fixedly mounted between the support plates 3; an inlet pipe 512, connected to one side of the raw material storage tank 511; a motor 513, located on the top of the raw material storage tank 511; a stirring shaft 514 mounted at the output end of the motor 513, the other end of the stirring shaft 514 movably penetrating the top of the raw material storage tank 511 and extending to the raw material storage tank 511. Inside, stirring rods 515 are equidistantly installed around the bottom of the outer wall of motor 513. A liquid outlet pipe 517 is connected to the bottom of the raw material storage tank 511. A filter cylinder 516 is threadedly connected to the bottom of the raw material storage tank 511. The liquid outlet pipe 517 extends into the interior of the filter cylinder 516. Fixing plates 518 are symmetrically fixed to the inner wall of the filter cylinder 516. A filter basket 519 is arranged between the fixing plates 518. A connecting pipe 5111 is connected to the bottom of the filter cylinder 516. A pressure sensing module 5114 is arranged at the bottom of the inner wall of the filter cylinder 516. A centrifugal pump 5112 is connected to the other end of the connecting pipe 5111. A connecting pipe 5113 is connected to the output end of the centrifugal pump 5112.

[0036] Furthermore, in this embodiment, by starting motor 513, the output end of motor 513 drives the stirring shaft 514 and the stirring rod 515 on the outer wall to rotate synchronously, stirring the fermentation liquid in the raw material storage tank 511 at low speed. After stirring, the fermentation liquid flows into filter cylinder 516 through the outlet pipe 517 at the bottom of the raw material storage tank 511. The fixing plate 518 inside the filter cylinder 516 supports the filter basket 519. When the fermentation liquid passes through the filter basket 519, large particulate bacterial aggregates and culture medium residues are intercepted. The fermentation liquid after preliminary filtration flows to the bottom of the filter cylinder 516. The pressure sensing module 5114 at the bottom of the inner wall of the filter cylinder 516 monitors the pressure inside the cylinder in real time. When the pressure is stable, the controller 2 starts the centrifugal pump 5112. The fermentation liquid after preliminary filtration enters the centrifugal pump 5112 through the connecting pipe 5111, and is then transported to the subsequent clarification component 6 through the connecting pipe 5113 at the output end of the centrifugal pump 5112.

[0037] Example 2:

[0038] Based on Embodiment 1, a preferred embodiment of the Escherichia coli fermentation liquid membrane separation and clarification device provided by this utility model is shown in Figures 1-4: The clarification component 6 includes: a filter component 61, disposed between the support frame 4 and the base plate 1; a fixing component 62, disposed on one side of the filter component 61; the filter component 61 includes: a fixing block 1 611, fixedly installed on the top of the inner wall of the support frame 4; a fixing block 2 615, fixedly installed on the top of the base plate 1; a filter cylinder 2 6111, evenly disposed between the fixing block 1 611 and the fixing block 2 615; a liquid inlet horizontal pipe 612 is provided at the bottom of the fixing block 1 611, one end of the liquid inlet horizontal pipe 612 is connected to the other end of the connecting pipe 2 5113 through a connector 613, and liquid inlet vertical pipes 614 are equidistantly connected at the bottom of the liquid inlet horizontal pipe 612; a liquid storage pipe 616 is fixedly installed on the top of the fixing block 2 615, and a drain pipe 617 is connected to one end of the liquid storage pipe 616. The top of the filter cartridge 611 is provided with support bases 618 at equal intervals. A first annular locking plate 619 is fixedly installed on the top of each support base 618. A second annular locking groove is opened at the bottom of the filter cartridge 6111, and a second annular locking groove is opened at the top of the filter cartridge 6111. A first connecting hole is opened inside the support base 618. A second connecting hole is opened at equal intervals on the top of the liquid storage tube 616. The first connecting hole and the second connecting hole are connected. The first annular locking plate 619 is engaged with the bottom of the second filter cartridge 6111 through the first annular locking groove. The bottom end of the second filter cartridge 6111 movably passes through the first connecting hole and the second connecting hole. A liquid inlet is opened at the top of the second filter cartridge 6111.

[0039] The bottom end of the liquid inlet vertical pipe 614 is movably connected to the filter cylinder 6111 through the liquid inlet hole. The filter cylinder 6111 is uniformly provided with flat sheet membranes 6112 inside.

[0040] Furthermore, in this embodiment, the annular locking groove at the bottom of the filter cylinder 6111 engages with the annular locking plate 619 at the top of the support base 618; the fermentation broth after preliminary filtration flows into the inlet horizontal pipe 612, and is then transported to the top of the corresponding filter cylinder 6111 through multiple sets of inlet vertical pipes 614 at the bottom of the inlet horizontal pipe 612. The bottom end of the inlet vertical pipe 614 extends into the cylinder through the inlet hole at the top of the filter cylinder 6111, where the flat plate membrane 6112 precisely filters the fermentation broth: the clarified liquid flows through the flat plate membrane 6112 to the bottom of the filter cylinder 6111, while impurities such as bacteria and large molecular proteins are trapped on the outside of the flat plate membrane 6112; the bottom of the filter cylinder 6111 is connected to the connection hole 2 of the storage pipe 616 through the connection hole 1 of the support base 618, and the clarified liquid flows into the storage pipe 616 and can be discharged through the drain pipe 617 at one end of the storage pipe 616.

[0041] Example 3:

[0042] Based on Embodiments 1 and 2, a preferred embodiment of the Escherichia coli fermentation liquid membrane separation and clarification device provided by this utility model is shown in Figures 1-4: The fixing component 62 includes: a fixing vertical plate 621, which is fixedly installed on one side of the support frame 4; a sliding opening is provided inside the fixing vertical plate 621; fixing plates 622 are symmetrically fixedly installed on the outer side of the fixing vertical plate 621; a motor 623 is provided at the top of the fixing plate 622; a threaded rod 624 is provided at the output end of the motor 623; the other end of the threaded rod 624 movably passes through the top and bottom of the fixing plate 622 and extends to the top of another fixing plate 622; a movable rod 625 is threadedly connected to the outer wall of the threaded rod 624; the other end of the movable rod 625 movably passes through the sliding opening and is fixedly installed with a clamping top cover 626; there are three sets of clamping top covers 626; the clamping top covers 626 are fixedly connected to each other by a connecting block 627; the clamping top covers 626... The top cover 626 is fixedly installed on the bottom of the liquid inlet vertical pipe 614 with an annular locking plate 628. The annular locking plate 628 is engaged with the top of the filter cylinder 6111 through the annular locking groove 628. The top cover 626 is adapted to the top of the filter cylinder 6111.

[0043] Furthermore, in this embodiment, a second motor 623 is fixed to a second fixed plate 622 on the outside of a fixed vertical plate 621, and the output end of the second motor 623 drives the threaded rod 624 to rotate; due to the movable rod 625 and

[0044] The threaded rod 624 is threaded, and the movable rod 625 moves downward along the threaded rod 624, causing the clamping top cover 626 to move downward synchronously; the clamping top cover 626 is movably sleeved on the outer wall of the liquid inlet vertical pipe 614, and the second annular locking plate 628 at its bottom is engaged with the second annular locking groove at the top of the second filter cylinder 6111.

[0045] During use, before the membrane separation process, filter cartridge 6111 needs to be fixed by fixing component 62. Motor 623 is then started and fixed to fixing plate 622 on the outside of fixing vertical plate 621. The output end of motor 623 drives threaded rod 624 to rotate. Since movable rod 625 is threadedly connected to threaded rod 624, movable rod 625 moves downwards along threaded rod 624, causing clamping top cover 626 to move downwards synchronously. Clamping top cover 626 is movably sleeved on the outer wall of liquid inlet vertical pipe 614. Its bottom annular locking plate 628 engages with the top annular locking groove 6111. Simultaneously, the bottom annular locking groove 6111 engages with the top annular locking plate 619 of support base 618, thus fixing filter cartridge 6111 vertically and preventing it from being damaged by liquid impact during separation. The E. coli fermentation broth to be processed is introduced into the raw material storage tank 511 through the inlet pipe 512. The operator starts the motor 513 through the controller 2 on the top of the base plate 1. The output end of the motor 513 drives the stirring shaft 514 to rotate synchronously with the stirring rod 515 on the outer wall, stirring the fermentation broth in the raw material storage tank 511 at low speed to prevent the bacterial cells from settling and stratifying, and to ensure that the concentration of the fermentation broth is uniform. After stirring, the fermentation broth flows into the filter cylinder 516 through the outlet pipe 517 at the bottom of the raw material storage tank 511. The filter cylinder 516 is threaded to the bottom of the raw material storage tank 511 for easy disassembly and maintenance. The fixing plate 518 inside the filter cylinder 516 supports the filter basket 519. When the fermentation broth passes through the filter basket 519, large bacterial aggregates and culture medium residues are intercepted. The fermentation broth after preliminary filtration flows to the bottom of the filter cylinder 516. The pressure sensing module 5114 at the bottom of the inner wall of the filter cylinder 516 monitors the pressure inside the cylinder in real time. When the pressure is stable, the controller 2... Centrifugal pump 5112 is started; the fermentation broth after preliminary filtration enters centrifugal pump 5112 through connecting pipe 1 5111, and is then transported to subsequent clarification component 6 through connecting pipe 2 5113 at the output end of centrifugal pump 5112; connecting pipe 2 5113 is connected to inlet horizontal pipe 612 through connector 613, and the fermentation broth after preliminary filtration flows into inlet horizontal pipe 612, and is transported to the top of the corresponding filter cylinder 2 6111 through multiple sets of inlet vertical pipes 614 at the bottom of inlet horizontal pipe 612. The bottom end of inlet vertical pipe 614 extends into the cylinder through the inlet hole at the top of filter cylinder 2 6111, and the flat plate membrane 6112 inside it precisely filters the fermentation broth.

[0046] Filtration: The clarified liquid flows through the flat plate membrane 6112 to the bottom of the filter cartridge 6111. Impurities such as bacteria and large molecular proteins are trapped on the outside of the flat plate membrane 6112. The bottom of the filter cartridge 6111 is connected to the connection hole 2 of the storage tube 616 through the connection hole 1 of the support 618. After the clarified liquid flows into the storage tube 616, it can be discharged through the drain pipe 617 at one end of the storage tube 616 and transported to the subsequent purification equipment to complete the core membrane separation and clarification process.

[0047] When membrane separation is complete or the flux of the flat sheet membrane 6112 decreases, the reverse motor 623 is started to move the clamping top cover 626 upward and disengage it from the filter cartridge 6111, so that the filter cartridge 6111 can be disassembled for cleaning or replacement of the flat sheet membrane.

[0048] At the same time, filter cartridge 516 can be unscrewed, filter basket 519 can be removed to clean the trapped impurities, and after completing the equipment maintenance, it can be reassembled and waited for the next processing cycle.

[0049] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A membrane separation and clarification device for Escherichia coli fermentation, comprising a base plate (1), characterized in that: A controller (2) is provided on the top of the base plate (1), a support plate (3) is symmetrically fixed on the top of the base plate (1), a support frame (4) is provided on the top of the base plate (1), the support frame (4) is located on one side of the support plate (3), a stirring assembly (5) is provided between the support plates (3), and a clarification assembly (6) is provided between the support frame (4) and the base plate (1).

2. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 1, characterized in that: The stirring assembly (5) includes: a raw material storage tank (511), which is fixedly installed between the support plates (3); The liquid inlet pipe (512) is connected to one side of the raw material storage tank (511); Motor 1 (513) is installed on top of the raw material storage tank (511).

3. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 2, characterized in that: The output end of the motor (513) is provided with a stirring shaft (514). The other end of the stirring shaft (514) movably passes through the top of the raw material storage tank (511) and extends into the interior of the raw material storage tank (511). Stirring rods (515) are equidistantly installed on the bottom circumference of the outer wall of the motor (513). The bottom of the raw material storage tank (511) is connected to a liquid outlet pipe (517). The bottom of the raw material storage tank (511) is threadedly connected to a filter cylinder (516). The liquid outlet pipe (517) extends into the filter cylinder (516). Inside the filter cylinder (516), a fixing plate (518) is symmetrically fixedly installed on the inner wall. A filter basket (519) is arranged between the fixing plates (518). A connecting pipe (5111) is connected to the bottom of the filter cylinder (516). A pressure sensing module (5114) is arranged at the bottom of the inner wall of the filter cylinder (516). A centrifugal pump (5112) is connected to the other end of the connecting pipe (5111). A connecting pipe (5113) is connected to the output end of the centrifugal pump (5112).

4. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 1, characterized in that: The clarification assembly (6) includes: a filter element (61) disposed between the support frame (4) and the base plate (1); The fixing component (62) is disposed on one side of the filter component (61).

5. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 4, characterized in that: The filter component (61) includes: a fixing block (611), which is fixedly installed on the top of the inner wall of the support frame (4); Fixing block two (615) is fixedly installed on the top of the base plate (1); The second filter cartridge (6111) is evenly arranged between the first fixed block (611) and the second fixed block (615).

6. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 5, characterized in that: The bottom of the first fixing block (611) is provided with a horizontal inlet pipe (612). One end of the horizontal inlet pipe (612) is connected to the other end of the second connecting pipe (5113) through a connector (613). The bottom of the horizontal inlet pipe (612) is provided with vertical inlet pipes (614) at equal intervals. The top of the second fixing block (615) is fixedly installed with a liquid storage pipe (616). One end of the liquid storage pipe (616) is connected with a drain pipe (617). The top of the liquid storage pipe (616) is provided with support seats (618) at equal intervals. The top of the support seat (618) is fixedly installed with an annular locking plate (619). The bottom of the second filter cylinder (6111) is provided with an annular locking groove. The top of the second filter cylinder (6111) is provided with an annular locking groove. The support base (618) is provided with an annular locking groove 2. An annular locking plate 1 (619) is fixedly installed on the top of the support base (618). A connection hole 1 is opened inside the support base (618). A connection hole 2 is opened at equal intervals on the top of the liquid storage tube (616). The connection hole 1 and the connection hole 2 are connected. The annular locking plate 1 (619) is locked and connected to the bottom of the filter cylinder 2 (6111) through the annular locking groove 1. The bottom end of the filter cylinder 2 (6111) movably passes through the connection hole 1 and the connection hole 2. A liquid inlet hole is opened on the top of the filter cylinder 2 (6111). The bottom end of the liquid inlet vertical pipe (614) is movably connected to the filter cylinder 2 (6111) through the liquid inlet hole. A flat membrane (6112) is uniformly arranged inside the filter cylinder 2 (6111).

7. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 4, characterized in that: The fixing component (62) includes a fixing vertical plate (621), which is fixedly installed on one side of the support frame (4).

8. The Escherichia coli fermentation liquid membrane separation and clarification device according to claim 7, characterized in that: The fixed vertical plate (621) has a sliding opening inside. A second fixed plate (622) is symmetrically fixedly installed on the outer side of the fixed vertical plate (621). A second motor (623) is installed on the top of the second fixed plate (622). A threaded rod (624) is installed at the output end of the second motor (623). The other end of the threaded rod (624) movably passes through the top and bottom of the second fixed plate (622) and extends to the top of another second fixed plate (622). A movable rod (625) is threadedly connected to the outer wall of the threaded rod (624). 5) The other end of the sliding port is movable through and fixedly installed with a clamping top cover (626). There are three sets of clamping top covers (626). The clamping top covers (626) are fixedly connected to each other by connecting blocks (627). The clamping top cover (626) is movably sleeved on the outer wall of the liquid inlet vertical pipe (614). The bottom of the clamping top cover (626) is fixedly installed with an annular locking plate two (628). The annular locking plate two (628) is locked to the top of the filter cylinder two (6111) through an annular locking groove two. The clamping top cover (626) is adapted to the top of the filter cylinder two (6111).

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