A dialysis bag fixing assembly for laboratory use and a dialysis device

Abstract

The utility model discloses a dialysis bag fixing assembly and dialysis device for laboratory belongs to separation and purification experimental equipment field. Dialysis bag fixing assembly includes the sealing cover of taking internal thread, outer support cylinder and inner support cylinder, is equipped with the sampling port and sensor insertion port on the sealing cover, and the outer support cylinder upper portion is equipped with the outer thread of cooperation with internal thread, and the inner support cylinder upper end is equipped with the flange of outward extension, is used for hanging and setting the inner support cylinder in the inside of outer support cylinder, and dialysis bag, inner support cylinder, outer support cylinder and sealing cover form dialysis bottle, and dialysis bag fixing assembly still can include the splicing type floating plate and / or spacing support for fixing dialysis bottle, and the splicing type floating plate is through the perforated sleeve and is set on spacing support. Dialysis device includes dialysis bag fixing assembly and box, and is equipped with pH sensor and conductivity sensor etc. in the box. The utility model can better satisfy the complex demand such as a small amount of sample processing, process condition groping, process characterization experimental design, and can improve experimental efficiency to satisfy actual demand.

Description

Technical Field

[0001] This utility model specifically relates to a laboratory dialysis bag fixing component and dialysis device, belonging to the field of separation and purification experimental equipment. Background Technology

[0002] Dialysis is a commonly used separation and purification technique in the research and development and manufacturing of biopharmaceuticals. The dialysis membrane involved has selective permeability. Typically, the dialysis membrane is made into a bag shape, and a solution of large biomolecules is placed inside the bag. The dialysis bag is then immersed in water or a buffer solution. Large biomolecules in the sample solution are retained inside the bag, while salts and smaller molecules continuously diffuse and dialyze to the outside of the bag until the concentrations inside and outside the bag reach equilibrium. The sample solution retained inside the dialysis bag that has not dialyzed out is called the "retention solution," and the solution outside the bag (membrane) is called the "exudate" or "dialysis solution."

[0003] In the preparation of biological macromolecules, dialysis is often used for desalting, removing excipients, removing small molecule impurities, and concentrating samples. Dialysis membranes with different molecular weight cutoffs can be selected according to different needs.

[0004] Currently, existing dialysis devices are mostly used to solve specific problems, such as: automatically controlling the dialysis fluid level by adding valves and controllers; and saving installation time by inserting dialysis cups onto the float body. However, none of these devices can meet the complex needs of biomacromolecule product research and development experiments.

[0005] Although existing technologies such as CN217614034U ("A Magnetic Stirring Dialysis Device") and CN217188880U ("A Laboratory-Specific Auxiliary Rapid Dialysis Device") are disclosed, they are not well-suited for research and development experiments on biomacromolecules, such as small-sample processing, process condition exploration, and process characterization experimental design. Furthermore, CN110331086A discloses "A Laboratory Dialysis Device for Studying Microbial Interactions," which primarily addresses the problem that ordinary laboratory dialysis devices cannot detect microbial interactions in real time, providing a suitable research device for studying the interactions between different bacterial cells during fermentation or culture.

[0006] Therefore, there is a need for a laboratory dialysis device that can meet the complex requirements of small sample processing, process condition exploration, and process characterization experimental design, and can improve experimental efficiency. Summary of the Invention

[0007] To overcome the shortcomings of existing technologies, a laboratory dialysis bag fixing component and dialysis device are proposed. In this technical solution, the enclosure, dialysis components, and control console effectively meet complex needs such as small-sample processing, process condition exploration, and experimental design for process characterization, while also improving experimental efficiency to satisfy practical requirements.

[0008] To achieve the above technical objectives, the following technical solution is proposed: The primary objective of this technical solution is to provide: a laboratory dialysis bag fixing assembly, comprising a sealing cap with internal threads, an outer support cylinder, and an inner support cylinder for supporting the dialysis bag, wherein the sealing cap is provided with a sampling port and a sensor insertion port, and an outer cap is fitted over both the sampling port and the sensor insertion port. The upper part of the outer support cylinder is provided with an external thread that mates with the internal thread; The inner support tube has an outwardly extending flange at its upper end, which is used to hang the inner support tube inside the outer support tube. During assembly, the dialysis bag can be placed inside the inner support tube. The opening of the dialysis bag is turned outward along the opening of the inner support tube towards the flange. The end of the turned-out dialysis bag is fixed to the outer wall of the inner support tube with fasteners (such as elastic rubber rings, ropes, etc.). The dialysis bag, inner support tube, outer support tube and sealing cap form a dialysis bottle (the dialysis bag, inner support tube, outer support tube and sealing cap are sealed by threads).

[0009] Preferably, the outer support tube is a perforated mesh tube, and the length of the outer support tube is greater than or equal to the length of the inner support tube. The length of the outer support tube can be freely selected according to the length of the dialysis bag, serving to protect the dialysis bag and also providing support to prevent it from rupturing due to excessive internal pressure. Preferably, the length of the outer support tube is greater than or equal to the length of the dialysis bag and greater than the length of the inner support tube. This limitation on the lengths of the three tubes can effectively fix and protect the dialysis bag without affecting the normal progress of dialysis, thus meeting complex needs such as process condition exploration and process characterization experimental design.

[0010] Preferably, the inner side of the sealing cover is provided with a sealing element to increase the sealing performance between the sealing cover and the outer support cylinder.

[0011] Preferably, the sealing element is an elastic rubber ring.

[0012] Preferably, the dialysis bag fixing assembly further includes a modular float plate, and the number of modular float plates is at least one. Each modular float plate is provided with a placement hole for fixing the dialysis bottle, and the outer diameter of the lower part of the outer support tube is less than the inner diameter of the placement hole and less than the outer diameter of the upper part formed by the combination of the outer support tube and the sealing cap, so that the dialysis bottle will not fall down from the modular float plate.

[0013] Preferably, the modular float is made of hollow plastic.

[0014] Preferably, the outer side of the modular float is provided with recessed slots and protruding inserts for splicing and assembling modular floats (so that they can be freely spliced ​​according to actual needs to meet the processing needs of different numbers of dialysis bags); in use, the modular float can be used as a single piece, or it can be composed of at least two modular floats spliced ​​together.

[0015] Preferably, the placement hole is located at the center of the modular float. This arrangement further improves the stability of the dialysis bag during placement. It also facilitates the assembly of the floats and ensures that the dialysis bags do not interfere with each other. Each modular float has 1 to 4 placement holes, evenly distributed across the floats. This arrangement allows multiple dialysis sessions to be performed simultaneously within the chamber, improving dialysis efficiency, and also facilitates the implementation of control experiments in experimental design.

[0016] Preferably, the dialysis bag fixing assembly further includes a limiting bracket, which includes at least two support pillars; at the same time, corresponding to the limiting bracket, the modular float is provided with perforations for fitting onto the limiting bracket. The specific location and number of perforations can be set according to actual needs. Preferably, there are four perforations, located at the four corners of the modular float, which facilitates the splicing and assembly of the modular floats and the fixing onto the limiting bracket. The function of the limiting bracket includes: (1) When the number of sensors inserted into the dialysis bag in the experiment is large, the buoyancy of the modular float may not be sufficient to support the dialysis bag and sensors to float above the liquid surface. In this case, fitting the perforations of the modular float onto the limiting bracket can provide support and prevent the dialysis bag from sinking below the liquid surface or tipping over. (2) After the experiment, the structure consisting of the limiting bracket, dialysis assembly and float plate can be moved out of the box as a whole. The limiting bracket and float plate can be used as dialysis bag support to support the sample, facilitate the draining of dialysis fluid, and prevent the dialysis fluid on the outer surface from contaminating the sample when it is removed.

[0017] Preferably, the limiting bracket has protrusions distributed longitudinally for fixing and limiting the modular float. Optionally, (1) the protrusions are made of elastic plastic (in the uninstalled state, the inner diameter of the protrusion is smaller than the outer diameter of the support column; in the installed state, since the protrusion is made of elastic material, the inner diameter of the protrusion can be slightly expanded and thus tightly fitted onto the support column, and can move up and down along the support column under the action of external force, so as to adjust the position of the modular float according to actual needs, and thus indirectly adjust the position of the dialysis bag); (2) the protrusions are integrally formed with the support column, and multiple protrusions are densely arranged on each support column, so as to flexibly adjust the position of the modular float according to needs.

[0018] The second objective of this technical solution is to provide a laboratory dialysis apparatus, comprising the aforementioned dialysis bag fixing assembly and a housing, wherein the housing is equipped with a temperature sensor, a liquid level sensor, a pH sensor I, a conductivity sensor I, a pH sensor II, and a conductivity sensor II. In use, a dialysis bag is inserted into the dialysis bag fixing assembly to form a dialysis bottle, and then the assembled dialysis bottle is placed into the housing.

[0019] Preferably, the outer side of the housing is fitted with an insulation jacket, and S-shaped copper coils are distributed inside the housing. An electric switching valve I is installed on the medium inlet pipe connected to the S-shaped copper coils, and an electric switching valve II is installed on the medium outlet pipe connected to the S-shaped copper coils. Refrigerant or heating medium can be connected externally as needed, and cooling or heating can be achieved according to the controller's instructions. Furthermore, the housing can be made of materials such as glass, plastic, or austenitic stainless steel, and its shape is preferably square.

[0020] Preferably, the lower part of the chamber is provided with a magnetic stirring mechanism, which includes a rotor isolation mesh and a magnetic rotor disposed within the rotor isolation mesh. The magnetic rotor inside the chamber is driven to rotate by magnetic force, thereby stirring the dialysate inside the chamber and accelerating the dialysis exchange rate.

[0021] Preferably, the housing is provided with a dialysate inlet, a dialysate outlet and an overflow port. The dialysate inlet is connected to a dialysate inlet pipe and a dialysate inlet pump is provided on the dialysate inlet pipe. A flow sensor is provided at the dialysate inlet and an outlet valve is provided at the dialysate outlet. The flow sensor, the inlet pump and the outlet valve are interlocked by an electrical signal.

[0022] Preferably, a control console is provided below the housing, and the control console is equipped with a controller and a human-machine interface (HMI). The controller is used to configure the control program, receive and process sensor data, and control the corresponding pumps and valves. The human-machine interface is used by the operator to perform stirring, temperature control, liquid level control, automatic program selection, parameter configuration, and operation. pH sensor I, conductivity sensor I, temperature sensor, liquid level sensor, pH sensor II, conductivity sensor II, flow sensor, inlet pump, outlet valve, electric switching valve I and / or electric switching valve II are connected to the controller via circuitry. Among them, pH sensor I is used to monitor the pH value of the sample in the dialysis bag online; when in use, its probe is placed in the sensor insertion port on the sealing cover of the dialysis bag fixing assembly; Conductivity sensor I: Used for online monitoring of sample concentration inside dialysis bags; when in use, place its probe into the sensor insertion port on the sealing cap of the dialysis bag fixing assembly; Temperature sensor: Used to monitor the temperature of the dialysate inside the chamber and feed it back to the controller in the control panel via circuitry. The controller controls the flow rate of the medium in the insulation jacket outside the chamber according to preset parameters to achieve temperature control. When in use, place its probe at the bottom of the chamber. Liquid level sensor: Used to monitor the liquid level of the dialysate in the tank and feed it back to the controller in the control panel through the circuit. The controller controls the inlet pump and outlet valve according to preset parameters to achieve liquid level control; when in use, place its probe at the bottom of the tank. pH Sensor II: Used for online monitoring of the pH value of the dialysis solution inside the chamber; when using, place its probe below the liquid surface inside the chamber (outside the dialysis bag); Conductivity sensor II: Used for online monitoring of the concentration of dialysis fluid inside the chamber; when in use, place its probe below the liquid surface inside the chamber (outside the dialysis bag); Flow sensor: Used to monitor the flow rate of dialysate at the dialysate inlet.

[0023] The positional relationships such as "inside", "between", "upper", "lower", "middle", "lower part", "outer side", and "longitudinal" involved in this technical solution are defined according to the actual usage conditions and are conventional terms in this technical field, as well as conventional terms used by those skilled in the art in actual use.

[0024] In the description of this technical solution, it should be noted that, unless otherwise explicitly specified and limited, the terms "setting" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0025] The beneficial technical effects of adopting this technical solution are as follows: This invention has a simple structure, is easy to operate and assemble, and can meet complex needs such as small sample processing, process condition exploration, and process characterization experimental design, greatly improving experimental efficiency and ultimately enabling laboratory use. Attached Figure Description

[0026] Figure 1 This is the front view of the present invention; Figure 2 This is a top view of the present invention; Figure 3 This is a schematic diagram of the structure of the float plate in this utility model; Figure 4 This is a schematic diagram of the structure after the float plates are spliced ​​together in this utility model; Figure 5 This is an assembly diagram of the dialysis bag, sealing cap, outer support tube, and inner support tube in this utility model; Figure 6 This is a control block diagram of the controller involved in this utility model; In the diagram, 10 is the flow sensor, 11 is the control console, 12 is the human-machine interface, 13 is the controller, 14 is the temperature sensor, 15 is the liquid level sensor, 16 is the pH sensor I, 17 is the conductivity sensor I, 18 is the circuit, 19 is the pH sensor II, and 20 is the conductivity sensor II. 21. Housing; 22. Dialysis fluid inlet; 23. S-shaped copper coil; 24. Dialysis fluid outlet; 25. Overflow port; 26. Magnetic rotor; 27. Rotor isolation screen; 28. Inlet pump; 29. ​​Outlet valve. 31. Assembleable floating plate; 311. Recessed slot; 312. Perforation; 313. Placement hole; 314. Protruding insert. 32. Limiting bracket; 33. Dialysis bag; 34. Outer support tube; 341. External thread; 342. Fastener; 343. Inner support tube; 35. Sampling port; 351. Outer cover; 352. Sensor insertion port; 36. Sealing cover; 361. Seal; 362. Internal thread; 37. Protrusion. 41. Dialysis fluid tank; 42. Refrigerant; 43. Heat transfer medium; 44. Electric switching valve I; 45. Electric switching valve II; 46. Transfer pump I; 47. Transfer pump II. Detailed Implementation

[0027] The technical solutions in the embodiments of this utility model will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0028] Example 1 This embodiment provides: a laboratory dialysis bag fixation assembly, such as... Figure 5 As shown, it includes a sealing cap 36 with internal threads 362, an outer support cylinder 34, and an inner support cylinder 343 for supporting the dialysis bag 33; (1) The sealing cover 36 is provided with a sampling port 35 and a sensor insertion port 352, and an outer cover 351 is fitted on the outside of both the sampling port 35 and the sensor insertion port 352; (2) The upper part of the outer support cylinder 34 is provided with an external thread 341 that mates with the internal thread 362; (3) The upper end of the inner support tube 343 is provided with an outwardly extending flange, which is used to hang the inner support tube 343 inside the outer support tube 34; During assembly, the dialysis bag 33 can be fitted inside the inner support tube 343. The opening of the dialysis bag 33 is turned outward along the opening of the inner support tube 343 towards the flange. The end of the turned-out dialysis bag 33 is fixed to the outer wall of the inner support tube 343 with a fastener 342 (a flexible rubber ring can be selected). The dialysis bag 33, the inner support tube 343, the outer support tube 34, and the sealing cap 36 form a dialysis bottle (the dialysis bag 33, the inner support tube 343, the outer support tube 34, and the sealing cap 36 are sealed by threads). This is beneficial for supporting and fixing the dialysis bag 33 to achieve the purpose of dialysis and provides a prerequisite guarantee for complex needs such as small sample processing, process condition exploration, and process characterization experimental design.

[0029] Example 2 Based on Example 1, this example further specifies the following to ensure better fit between the components in the dialysis bag fixing assembly: The outer support tube 34 is a perforated mesh tube, and its length is greater than or equal to the length of the inner support tube 343. The length of the outer support tube 34 can be freely selected based on the length of the dialysis bag 33. It serves to protect the dialysis bag 33 and also provides support, preventing it from rupturing due to excessive internal pressure. Preferably, the length of the outer support tube 34 is greater than or equal to the length of the dialysis bag 33, and greater than the length of the inner support tube 343. That is, the length of the dialysis bag 33 is between the lengths of the outer and inner support tubes. Therefore, limiting the lengths of the outer and inner support tubes 34 and 343 effectively secures and protects the dialysis bag 33 without affecting the normal dialysis process, thus meeting complex requirements such as process condition exploration and process characterization experimental design.

[0030] In addition, the inner side of the sealing cover 36 is provided with a sealing element 361 for increasing the sealing performance between the sealing cover 36 and the outer support cylinder 34. In one embodiment, the sealing element 361 is an elastic rubber ring.

[0031] In use, a pH sensor I16 and / or a conductivity sensor I17 can be inserted into the sensor insertion port 352. The pH sensor I16 is used for online monitoring of the pH value of the sample inside the dialysis bag 33, and the conductivity sensor I17 is used for online monitoring of the concentration of the sample inside the dialysis bag 33.

[0032] Example 3 Based on Examples 1-2, this example further enhances the stability of the dialysis bag fixing assembly, indirectly improving the stability of the dialysis bag 33 installation, so as to smoothly and stably complete the dialysis experiment. The following limitations are further specified: like Figure 1-4As shown, the dialysis bag fixing assembly also includes a modular float 31. The modular float 31 has a placement hole 313 for fixing the dialysis bottle. The outer diameter of the lower part of the outer support tube 34 is less than the inner diameter of the placement hole 313 and less than the outer diameter of the upper part formed by the combination of the outer support tube 34 and the sealing cap 36. In use, the outer support tube 34 is placed into the placement hole 313 of the modular float 31, with its upper part located on the upper side of the modular float 31 and its lower part extending through the placement hole 313 towards the bottom of the housing 21, so that the dialysis bag 33 will not fall down from the modular float 31. Preferably, the placement hole 313 is located at the center of the modular float 31. This setting further improves the stability of the dialysis bag 33 and not only facilitates the splicing and assembly of the floats, but also ensures that the dialysis bags 33 do not interfere with each other. There are 1 to 4 placement holes 313, which are evenly distributed on the modular float 31. This arrangement can ensure that multiple dialysis sessions can be performed simultaneously in the chamber 21, thereby improving dialysis efficiency; and it also facilitates the implementation of control experiments during experimental design. In one specific embodiment, the modular float 31 is made of hollow plastic (the specific material can be ethylene-vinyl acetate copolymer, polypropylene, etc.). The outer side of the modular float 31 is provided with recessed slots 311 and protruding inserts 314 for splicing and assembling the modular floats 31. Figure 3 As shown), it can be freely assembled according to actual needs to meet the processing requirements of different numbers of dialysis bags 33. The modular float 31 can be used individually or assembled from at least two modular floats 31 (as shown). Figure 4 (As shown).

[0033] Example 4 Based on Example 3, this example further limits the following to improve the stability of the assembled float 31 installation, thereby indirectly improving the stability of the dialysis bag 33 installation, and thus ensuring the smooth and stable completion of the dialysis experiment: like Figure 1 As shown, the dialysis bag fixing assembly also includes a limiting bracket 32, which includes at least two support pillars. Correspondingly, the modular float 31 has perforations 312 distributed on it for fitting onto the limiting bracket 32. When the experiment requires a large number of sensors to be inserted into the dialysis bag 33, the buoyancy of the modular float 31 may not be sufficient to support the dialysis bag 33 and the sensors to float above the liquid surface. In this case, the limiting bracket 32 ​​can provide stronger support and prevent the dialysis bag 33 from sinking below the liquid surface or tipping over. The specific location and number of perforations 312 can be further selected according to actual needs. In one specific implementation, there are four perforations 312, which are located at the four corners of the modular floating plate 31. This facilitates the splicing and combination of the modular floating plates 31 and the fixing on the limiting bracket 32.

[0034] In a preferred embodiment, each support of the limiting bracket 32 ​​has at least one protrusion 37 distributed longitudinally. The protrusion 37 mates with the perforation 312 on the modular float 31. The outer diameter of the protrusion 37 is larger than the inner diameter of the perforation 312, thus the protrusion 37 can be used to support the modular float 31. In use, the perforation 312 is simply fitted over the protrusion 37 of the limiting bracket 32. In a more preferred embodiment, the height of the protrusion 37 on the limiting bracket 32 ​​can be adjusted vertically. Therefore, the height of the protrusion 37 on the limiting bracket 32 ​​can be adjusted and fixed according to actual needs, thereby indirectly adjusting the height of the modular float 31 and the dialysis bag 33 to complete the dialysis experiment.

[0035] In practical implementation, it should be noted that: 1. When the limit bracket does not have a protrusion: The inner diameter of the perforation 312 of the modular float 31 is slightly smaller than the outer diameter of the limiting bracket 32. Due to the elasticity of the material of the modular float 31, the perforation 312 can be slightly expanded to fit tightly onto the limiting bracket 32, and a certain frictional force is generated between the contact surfaces of the perforation 312 and the limiting bracket 32. Therefore, the weight of the modular float 31 and the dialysis bottle is balanced by the buoyancy of the dialysis fluid and the aforementioned frictional force, so that the modular float 31 can be fixed at a specific position (i.e., a specific height) on the limiting bracket 32. When it is necessary to adjust the position of the modular float 31 on the limiting bracket 32, a vertical external force (such as the pushing force of a hand) is applied to the modular float 31. With the help of the elasticity of the material of the modular float 31, the perforation can be adjusted up and down along the limiting bracket 32, and stay at a suitable height after the external force is removed. Finally, the position of the modular float 31 in the box is adapted.

[0036] II. When the limiting bracket 32 ​​has a protrusion 37, there are two situations: 1) The position of the protrusion 37 on the limiting bracket 32 ​​is fixed (e.g., the protrusion 37 and the limiting bracket 32 ​​are integrally formed): Each support column of the limiting bracket 32 ​​is provided with multiple protrusions 37, which are densely distributed on the limiting bracket 32 ​​to provide multiple optional stopping positions for the modular float 31. The inner diameter of the perforation 312 of the modular float 31 is slightly smaller than the outer diameter of the protrusions 37. Therefore, the weight of the modular float 31 and the dialysis bottle is balanced by the buoyancy of the dialysis fluid and the supporting force of the protrusions 37, so that the modular float 31 can be fixed at a specific position (i.e., a specific height) on the limiting bracket 32. When it is necessary to adjust the position of the modular float 31 on the limiting bracket 32, a vertical external force (such as the pushing force of a hand) is applied to the modular float 31. With the help of the elasticity of the material of the modular float 31, the perforation 312 can be slightly expanded and pass over the protrusion 37 to adjust the vertical position. After the external force is removed, it stays above the appropriate protrusion 37. Finally, the position of the modular float 31 in the box 21 is adapted.

[0037] 2) The position of the protrusion 37 on the limiting bracket 32 ​​is adjustable (e.g., the protrusion 37 made of elastic material is fitted onto the limiting bracket 32): Protrusion 37 is a protrusion made of polyurethane, ethylene propylene rubber, fluororubber or silicone rubber elastomer material.

[0038] Each support column of the limiting bracket 32 ​​can be provided with a protrusion 37. When not installed, the inner diameter of the protrusion 37 is slightly smaller than the outer diameter of the limiting bracket 32. With the help of the elasticity of the material of the protrusion 37, the protrusion 37 can expand slightly to fit on the specific position of the limiting bracket 32, and can move up and down along the limiting bracket 32 ​​under the action of external force, so as to provide a variety of optional parking positions for the modular floating plate 31. The inner diameter of the perforation 312 of the modular float 31 is slightly smaller than the outer diameter of the protrusion 37. Therefore, the weight of the modular float 31 and the dialysis bottle is balanced by the buoyancy of the dialysis fluid and the supporting force of the protrusion 37, so that the modular float 31 can be fixed at a specific position (i.e., a specific height) on the limiting bracket 32.

[0039] When it is necessary to adjust the position of the modular float 31 on the limiting bracket 32, a vertical external force (such as the pushing force of a hand) is applied to the protrusion 37. With the help of the elasticity of the material of the protrusion 37, the protrusion 37 can move up and down along the limiting bracket 32, thereby providing different parking positions for the modular float 31.

[0040] Example 5 Based on Examples 1-4, this example provides a laboratory dialysis device, such as... Figure 1 As shown, the system includes the aforementioned dialysis bag fixing assembly and housing 21. The housing 21 houses a temperature sensor 14, a liquid level sensor 15, a pH sensor I 16, a conductivity sensor I 17, a pH sensor II 19, and a conductivity sensor II 20. This setup, based on the coordinated arrangement of the housing 21 and the dialysis bag fixing assembly, allows for the fulfillment of complex needs such as small-scale sample processing, process condition exploration, and process characterization experimental design without affecting the normal operation of dialysis.

[0041] The operating method of the laboratory dialysis apparatus is as follows: (1) When dialysis is required, the dialysis bag 33 is inserted into the dialysis bag fixing assembly to form a dialysis bottle, and then the assembled dialysis bottle is placed into the box 21. Specifically, one of the following methods can be adopted: A. When using only the modular float plate 31 to support the dialysis bottle, the following approach (a1) or (a2) can be adopted: a1) First, place the modular float 31 into the box 21 (floating on the liquid surface), and then insert the dialysis bottle into the placement hole 313 of the modular float 31; a2) First, insert the dialysis bottle into the placement hole 313 of the modular float 31, and then put the whole bottle into the box 21. B. When using the modular float 31 and the limiting bracket 32 ​​to support the dialysis bottle, any one of the following methods (b1) to (b4) can be adopted: b1) First, the modular float 31 is fitted onto the limiting bracket 32 ​​through the perforation 312. Then, the dialysis bottle is placed into the placement hole 313 of the modular float 31. The dialysis bottle, modular float 31 and limiting bracket 32 ​​are then assembled and placed into the box 21. The height of the modular float 31 can be further adjusted as needed to make it flush with the liquid surface. b2) First, place the dialysis bottle into the placement hole 313 of the modular float 31. Then, fit the modular float 31 into the appropriate position on the limiting bracket 32 ​​through the perforation 312. Then, put the dialysis bottle, modular float 31 and limiting bracket 32 ​​into the box 21 as a whole. The height of the modular float 31 can be further adjusted as needed to make it flush with the liquid surface. b3) First, place the limiting bracket 32 ​​into the box 21, then put the modular float 31 into the appropriate position on the limiting bracket 32 ​​through the perforation 312 (so that it is flush with the liquid surface), and then put the assembled dialysis bottle into the placement hole 313 of the modular float 31. b4) First, place the limiting bracket 32 ​​into the box 21, and then put the combiable float 31, which has been placed into the dialysis bottle, into the appropriate position on the limiting bracket 32 ​​through the perforation 312 (so that the combiable float 31 is flush with the liquid surface).

[0042] (2) After the dialysis experiment is completed, the dialysis bottle is removed from the box 21. Specifically, one of the following methods can be adopted: i) directly remove the dialysis bottle from the placement hole 313 of the modular float 31; or ii) first remove the dialysis bottle and modular float 31 as a whole from the box 21, and then remove the dialysis bottle from the modular float 31; or iii) first remove the dialysis bottle, modular float 31 and limiting bracket 32 ​​as a whole from the box 21 (at this time, the limiting bracket 32 ​​and modular float 31 can be used as a support for the dialysis bag 33, which can continue to support the sample, facilitate the draining of dialysis fluid, and at the same time avoid the outer surface of the sample being contaminated by dialysis fluid when the sample is removed), and then remove the dialysis bottle in time.

[0043] Example 6 Based on Example 5, this example further defines the enclosure 21 to ensure the smooth and effective operation of dialysis: The outer side of the housing 21 is fitted with an insulation jacket. S-shaped copper coils 23 are distributed inside the housing 21. An electric switching valve I44 and a delivery pump II47 are installed on the inlet pipe connected to the S-shaped copper coils 23, and an electric switching valve II45 and a delivery pump I46 are installed on the outlet pipe connected to the S-shaped copper coils 23. Refrigerant 42 or heating medium 43 can be connected externally as needed to achieve cooling or heating according to the instructions of the controller 13. Furthermore, the housing 21 can be made of materials such as glass, plastic, or austenitic stainless steel, and its shape is preferably square.

[0044] The housing 21 is equipped with a dialysate inlet 22, a dialysate outlet 24, and an overflow port 25. The dialysate inlet 22 is connected to the dialysate tank 1 through a dialysate inlet pipe, and an inlet pump 28 is provided on the dialysate inlet pipe. A flow sensor 10 is provided at the dialysate inlet 22, and an outlet valve 29 is provided at the dialysate outlet 24. The flow sensor 10, the inlet pump 28, and the outlet valve 29 are interlocked by an electrical signal.

[0045] The lower part of the housing 21 is equipped with a magnetic stirring mechanism, which includes a rotor isolation mesh 27 and a magnetic rotor 26 disposed within the rotor isolation mesh 27. The magnetic rotor 26 inside the housing 21 is driven to rotate by magnetic force, thereby stirring the dialysate inside the housing 21 and accelerating the dialysis exchange rate.

[0046] Example 7 Based on Examples 5-6, this example further specifies the following to improve the automation of the dialysis experiment, reduce human error, and increase experimental efficiency: like Figure 1As shown, a control console 11 is provided below the housing 21 (which can use the Siemens STEP 7 + WinCC solution, pre-configured with control modules such as heating, cooling, heat preservation, liquid inlet, liquid outlet, stirring, pH monitoring, conductivity monitoring, liquid level monitoring, and flow monitoring, which can be combined through sequential control program (SFC) according to different usage scenarios to form multiple automatic control programs; the control console 11 is equipped with a controller 13 and a human-machine interface 12).

[0047] Among them, controller 13 is used to configure the control program, receive and process sensor data, and control the corresponding pumps and valves. Examples include: Mettler M300 Process multi-parameter transmitter, Nanjing Wotian intelligent digital level display controller, and Siemens S7-200SMART PLC. Human-Machine Interface 12: Used by operators for functions such as stirring, temperature control, liquid level control, selection of automatic programs, parameter configuration, and operation. Example: Siemens Smart 700 IE V3 panel; like Figure 6 As shown, pH sensor I 16, conductivity sensor I 17, temperature sensor 14, liquid level sensor 15, pH sensor II 19, conductivity sensor II 20, flow sensor 10, inlet pump 28, outlet valve 29, electric switching valve I 44 and electric switching valve II 45 are all connected to controller 13 through circuit 18. Among them, pH sensor I16 is used for online monitoring of the pH value of the sample inside dialysis bag 33. For example, the Mettler InLabUltra Micro-ISM pH sensor; Conductivity sensor I17: Used for online monitoring of sample concentration within dialysis bag 33. Example: Mettler ISM 1.5" TRI .1C SS conductivity sensor; Temperature sensor 14: Used to monitor the temperature of the dialysate inside the chamber 21, and fed back to the controller 13 in the control console 11 via circuit 18. The controller 13 controls the flow rate of the medium in the outer insulation jacket of the chamber 21 according to preset parameters to achieve temperature control. For example: Nanjing Wotian PT10 leaded platinum resistance thermometer; Liquid level sensor 15: Used to monitor the liquid level of the dialysate in the tank 21 and feeds it back to the controller 13 in the control console 11 via circuit 18. The controller 13 controls the inlet pump 28 and the outlet valve 29 according to preset parameters to achieve liquid level control. For example: Nanjing Wotian PCM269; pH Sensor II19: Used for online monitoring of the pH value of the dialysis solution inside chamber 21. For example, the Mettler InLab UltraMicro-ISM pH sensor; Conductivity sensor II20: Used for online monitoring of the dialysate concentration inside chamber 21. For example: Mettler ISM 1.5" TRI .1C SS conductivity sensor; Flow sensor 10: Used to monitor the flow rate of dialysate at dialysate inlet 22. For example, the LianCe FMX-240 electromagnetic flow meter.

[0048] The number and specific location of pH sensor I16, conductivity sensor I17, temperature sensor 14, liquid level sensor 15, pH sensor II19, conductivity sensor II20, flow sensor 10, inlet pump 28, outlet valve 29, and electric switching valve can be further adjusted according to actual needs.

[0049] Application Example 1 Based on Examples 1-6, and according to experimental requirements: a small amount of sample (e.g., two dialysis bottles) is dialyzed overnight at a temperature of 4-6°C, without monitoring parameters such as conductivity. Specifically, this includes: 1. When using the dialysis bottle, depending on the requirements, do not insert the pH sensor I16 and / or conductivity sensor I17. Instead, close the outer cover 351 outside the sensor insertion port 352, select a dialysis bag 33 and an outer support tube 34 of appropriate length, assemble the dialysis bag 33 and the inner support tube 343 using the elastic rubber ring, place it inside the outer support tube 34, add the sample to the dialysis bag 33, and tighten the sealing cap 36. 2. Place the assembled dialysis bottles into the placement holes 313 on the modular float 31, arranging the two dialysis bottles diagonally. Inject dialysis fluid into the housing 21, and then place the modular float 31 containing the dialysis bottles into the housing 21. The modular float 31 will float on the liquid surface. 3. Set the temperature to 5℃ on the human-machine interface of the control console 11, with a temperature range of 4-6℃. Adjust the flow rate of refrigerant 42 on the controller 13 to control the temperature of the dialysate to the predetermined range. Start the magnetic stirring and let it sit overnight.

[0050] Application Example 2 Based on Examples 1-6, and according to experimental requirements: dialysis process conditions were explored, the experimental samples involved two dialysis bottles, the dialysis time for a macromolecule to reach the expected standard was studied, the dialysis temperature was 2-8℃, and conductivity parameters were monitored. Specifically, this included: 1. Select a sealing cap 36 with a sampling port 35 and a sensor insertion port 352 for one dialysis bottle. Insert the sampling needle of an automatic sampler into the sampling port 35 and insert a conductivity probe of a conductivity sensor I17 into the sensor insertion port 352. 2. Multiple modular floats 31 are spliced ​​together to provide sufficient buoyancy to support the weight of the modular floats 31, the conductivity probe and the sampling needle. 3. Place the assembled dialysis bottle into the placement hole 313 near the center of the modular float 31, with the two dialysis bottles placed diagonally. Inject dialysis fluid into the box 21, and then place the modular float 31 containing the dialysis bottle into the box 21. The modular float 31 will float on the liquid surface. 4. Place the conductivity probe of a conductivity sensor II20 into the dialysate inside the chamber 21; set the temperature to 5℃ on the human-machine interface of the control console 11, with a temperature range of 2-8℃; adjust the flow rate of refrigerant 42 of the controller 13 to control the temperature of the dialysate to the predetermined range; start the magnetic stirring; and monitor the conductivity inside and outside the dialysate bottle at the same time.

[0051] The control program is set to simultaneously introduce and drain dialysate into the chamber 21, ensuring continuous dialysate replacement and maintaining the dialysis temperature within the range of 2-8℃ to achieve continuous dialysis. The human-machine interface 12 records conductivity data every 10 minutes, and the automatic sampler takes samples every 4 hours.

[0052] By analyzing conductivity data and automatically timed sample collection, the goal of exploring efficient processes can be achieved.

[0053] Application Example 3 Based on Examples 1-6, and according to experimental requirements: dialysis process conditions were explored, involving six dialysis bottles. The study investigated the dosage of dialysis solution for three different macromolecules to achieve the expected standards (one macromolecule's stability was pH-sensitive). Dialysis was performed at room temperature, and conductivity parameters and dialysis solution pH were monitored. Specifically, this included: (1) Select a sealing cap 36 with a sampling port 35 and two sensor insertion ports 352 for the three dialysis bottles. Insert the sampling needle of the automatic sampler into the sampling port 35, insert the conductivity probe of conductivity sensor I 17 into one sensor insertion port 352, and insert the pH probe of pH sensor I 16 into the other sensor insertion port 352 (or, you can choose to insert the sampling needle into the sampling port 35 and the probe of conductivity sensor I 17 and / or pH sensor I 16 into the sensor insertion port 352 after step (3) below, for easy operation and fixation). (2) Use multiple modular floating plates 31 to splice them together, and insert limiting brackets 32 into the perforations 312 at the four corners of the modular floating plates 31 respectively, and adjust the height to a suitable height; (3) Place the assembled dialysis bottles in the placement hole 313 near the center of the modular float 31, with the dialysis bottles placed in sequence; inject dialysis fluid into the box 21, and then place the modular float 31 (together with the limiting bracket 32) containing the dialysis bottles into the box 21. The modular float 31 floats on the liquid surface, and is supported by the limiting bracket 32. (4) Place the pH probe of pH sensor II19 into the dialysis solution and the conductivity probe of conductivity sensor II20 into the dialysis solution; start the magnetic stirring mechanism and monitor the conductivity inside the dialysis bottle, the conductivity outside the dialysis bottle, and the pH value of the dialysis solution at the same time. The control program is set as follows: the outlet valve 29 at the dialysate outlet 24 is opened every 1 hour to discharge the dialysate. When the liquid level is lower than the set value, the magnetic stirring mechanism and outlet valve 29 are closed, and the inlet pump 28 at the dialysate inlet 22 is started; when the liquid level is higher than the set value, the magnetic stirring mechanism is started, and the inlet pump 28 is closed. The human-machine interface 12 records conductivity data and pH value every 10 minutes, and the automatic sampler takes samples every 4 hours.

[0054] Ultimately, by analyzing conductivity, pH value, and automatically timed sample collection, the goal of exploring efficient processes for simultaneously processing three macromolecules was achieved.

[0055] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A laboratory dialysis bag fixing assembly, characterized in that, It includes a sealing cap (36) with internal threads (362), an outer support cylinder (34) and an inner support cylinder (343) for supporting the dialysis bag (33). The sealing cap (36) is provided with a sampling port (35) and a sensor insertion port (352). Both the sampling port (35) and the sensor insertion port (352) are covered with an outer cap (351). The upper part of the outer support cylinder (34) is provided with an external thread (341) that mates with the internal thread (362); The inner support tube (343) has an outwardly extending flange at its upper end, which is used to hang the inner support tube (343) inside the outer support tube (34).

2. The laboratory dialysis bag fixing assembly according to claim 1, characterized in that, The outer support tube (34) is a hollow mesh tube, and the length of the outer support tube (34) is greater than or equal to the length of the inner support tube (343).

3. The laboratory dialysis bag fixing assembly according to claim 1, characterized in that, The inner side of the sealing cover (36) is provided with a sealing element (361) to increase the sealing performance between the sealing cover (36) and the outer support cylinder (34).

4. The laboratory dialysis bag fixing assembly according to claim 3, characterized in that, The sealing element (361) is an elastic rubber ring.

5. The laboratory dialysis bag fixation assembly according to any one of claims 1-4, characterized in that, The dialysis bag fixing assembly also includes a modular float (31), which has a placement hole (313). The outer diameter of the lower part of the outer support tube (34) is less than the inner diameter of the placement hole (313) and less than the outer diameter of the upper part formed by the combination of the outer support tube (34) and the sealing cap (36).

6. The laboratory dialysis bag fixation assembly according to claim 5, characterized in that, The placement hole (313) is located at the center of the modular float (31), and there are 1 to 4 placement holes (313) evenly distributed on the modular float (31).

7. The laboratory dialysis bag fixing assembly according to claim 5, characterized in that, The modular float (31) is a modular float made of hollow plastic.

8. The laboratory dialysis bag fixation assembly according to claim 5, characterized in that, The outside of the modular floating plate (31) is provided with a recessed slot (311) and a protruding insert (314) for splicing and assembling between modular floating plates (31).

9. The laboratory dialysis bag fixing assembly according to claim 5, characterized in that, The dialysis bag (33) fixing assembly also includes a limiting bracket (32), which includes at least two support pillars; the modular float (31) has perforations (312) distributed on it for fitting onto the limiting bracket (32).

10. The laboratory dialysis bag fixing assembly according to claim 9, characterized in that, The limiting bracket (32) has protrusions (37) distributed longitudinally for fixing and limiting the modular floating plate (31).

11. A laboratory dialysis apparatus, characterized in that, The dialysis bag fixing assembly and housing (21) as described in any one of claims 1-10 are provided in the housing (21), which is equipped with a temperature sensor (14), a liquid level sensor (15), a pH sensor I (16), a conductivity sensor I (17), a pH sensor II (19) and a conductivity sensor II (20).

12. The dialysis apparatus according to claim 11, characterized in that, The outer side of the box (21) is fitted with an insulation jacket, and S-shaped copper coils (23) are distributed inside the box (21). An electric switching valve I (44) is provided on the medium inlet pipe connected to the S-shaped copper coils (23), and an electric switching valve II (45) is provided on the medium outlet pipe connected to the S-shaped copper coils (23).

13. The dialysis apparatus according to claim 11, characterized in that, The lower part of the box (21) is provided with a magnetic stirring mechanism, which includes a rotor isolation net (27) and a magnetic rotor (26) disposed in the rotor isolation net (27).

14. The dialysis apparatus according to any one of claims 11-13, characterized in that, The housing (21) is provided with a dialysate inlet (22), a dialysate outlet (24) and an overflow port (25). The dialysate inlet (22) is connected to a dialysate inlet pipe, and a dialysate inlet pump (28) is provided on the dialysate inlet pipe. A flow sensor (10) is provided at the dialysate inlet (22), and a drain valve (29) is provided at the dialysate outlet (24). The flow sensor (10), the drain pump (28) and the drain valve (29) are interlocked by electrical signals.

15. The dialysis apparatus according to claim 14, characterized in that, The lower part of the housing (21) is provided with a control console (11), and the control console (11) is provided with a controller (13) and a human-machine interface (12); pH sensor I (16), conductivity sensor I (17), temperature sensor (14), liquid level sensor (15), pH sensor II (19), conductivity sensor II (20), flow sensor (10), inlet pump (28), outlet valve (29), electric switching valve I (44) and / or electric switching valve II (45) are connected to the controller (13) through circuit (18).

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