Magnetic bead method trace DNA extraction kit

Abstract

The utility model relates to a magnetic bead method trace DNA extraction kit. The structure includes: reagent box main part, sealing cover, sheltering subassembly, first adhesive piece and second adhesive piece, the reagent box main part is the rectangular casing structure of top opening, the reagent box main part top installs sealing cover, through the installation sealing cover and sheltering subassembly, sealing cover is close to reagent box main part, first adhesive piece and second adhesive piece mutually stick, sealing cover fixed mounting is in the top of reagent box main part, carries out the sealing protection to the top of reagent box main part, sheltering subassembly is close to reagent box main part, third adhesive piece and fourth adhesive piece stick, the finger of convenient detection personnel passes through multiple handheld components, lifts multiple reagent box main parts once, separates sealing cover and reagent box main part, third adhesive piece and first adhesive piece stick, sheltering subassembly carries out the sheltering protection to first adhesive piece, prevents first adhesive piece to scratch to detection personnel, influences the experience of grabbing.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, and more particularly to a magnetic bead-based trace DNA extraction kit. Background Technology

[0002] Magnetic bead-based trace DNA extraction is a core technology based on the principle of functional modification of magnetic microspheres and specific binding to DNA. It uses an external magnetic field to drive the rapid purification of DNA from trace biological samples (such as contact exfoliated cells, saliva stains, hair roots, bone fragments, etc.). It has irreplaceable application value in forensic evidence science, criminal investigation, and genetic research. Through the synergistic effect of magnetic separation carriers and targeted capture mechanisms, this technology breaks through the limitations of traditional DNA extraction methods on sample volume and integrity. It is especially suitable for processing trace (≤1 ng), degraded (fragment <300 bp), or complex matrix (such as soil, dye contamination) samples.

[0003] Current magnetic bead-based trace DNA extraction kits have a design flaw that affects ease of use. Because the surface lacks a gripping structure, operators must open their palms to grasp the kits, and only one kit can be moved at a time. This single-handed grasping method is extremely inconvenient when multiple kits need to be moved efficiently, wasting considerable time and effort and significantly reducing overall operational efficiency. This design shortcoming prevents the kits from meeting the demands for rapid and efficient use in practical applications, necessitating improvements through optimized design and the addition of gripping structures.

[0004] Therefore, a new magnetic bead-based trace DNA extraction kit is proposed to address the above problems. Utility Model Content

[0005] To overcome the problems existing in related technologies, this utility model provides a magnetic bead-based trace DNA extraction kit, which can be equipped with a handle component. By passing a finger through the handle component, multiple kits can be grasped at once for transportation, thus improving the convenience of the kit.

[0006] To achieve the above objectives, the first aspect of this utility model provides a magnetic bead-based trace DNA extraction kit, comprising: The reagent kit body, sealing cap, shielding assembly, first adhesive sheet, and second adhesive sheet; The reagent kit body is a rectangular shell structure with an open top. A sealing cap is installed at the top of the reagent kit body. A shielding component is symmetrically fixedly connected to the reagent kit body. A first adhesive sheet is fixedly connected to the surface of the reagent kit body near the shielding component. A second adhesive sheet is symmetrically fixedly connected to the lower surface of the sealing cap. The first adhesive sheet and the second adhesive sheet are bonded to each other. A handheld component is fixedly connected to the shielding component.

[0007] Furthermore, a third adhesive piece is fixedly connected to the lower surface of the shielding component, and the third adhesive piece is bonded to the first adhesive piece.

[0008] Furthermore, a fourth adhesive piece is symmetrically fixedly connected to the upper surface of the sealing cover, and the fourth adhesive piece is bonded to the third adhesive piece.

[0009] Furthermore, a positioning block is provided between the two second adhesive sheets and is fixedly connected to the lower surface of the sealing cap. A sealing gasket is fixedly connected to the side of the positioning block, and the sealing gasket is in contact with the inner wall of the reagent kit body.

[0010] Furthermore, a handle frame is fixedly connected inside the handheld component.

[0011] Furthermore, an adjustment mounting base is fixedly connected to the inner wall of the reagent kit body. Magnetic strip bodies are equidistantly arranged on the adjustment mounting base. Limiting plates are rotatably connected to both sides of the adjustment mounting base, and the limiting plates are engaged with the magnetic strip bodies.

[0012] Furthermore, a rack plate is symmetrically slidably connected inside the adjusting mounting base, and a gear rod is fixedly connected to the limiting plate. The rack plate and the gear rod are meshed together. A two-way lead screw is rotatably connected inside the adjusting mounting base, and the two-way lead screw is meshed with the rack plate.

[0013] The technical solution provided by this utility model can include the following beneficial effects: In this example, by installing a sealing cap and a shielding component, the sealing cap is placed close to the reagent kit body. The first adhesive sheet and the second adhesive sheet are bonded to each other. The sealing cap is fixedly installed on the top of the reagent kit body to seal and protect the top of the reagent kit body. The shielding component is placed close to the reagent kit body, and the third adhesive sheet and the fourth adhesive sheet are bonded together. This allows the testing personnel to easily lift multiple reagent kit bodies at once by passing their fingers through multiple handheld components, separating the sealing cap from the reagent kit body. The third adhesive sheet is bonded to the first adhesive sheet, and the shielding component shields and protects the first adhesive sheet to prevent it from scratching the testing personnel and affecting the gripping experience.

[0014] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the present invention. Attached Figure Description

[0015] The above and other objects, features and advantages of the present invention will become more apparent from the accompanying drawings, in which like reference numerals generally represent like parts.

[0016] Figure 1 This is a schematic diagram of the overall structure from one angle shown in one embodiment of this utility model; Figure 2This is a schematic diagram of the overall structure from another angle, as shown in an embodiment of the present invention; Figure 3 This is a schematic diagram of one of the angle-split structures shown in an embodiment of the present utility model; Figure 4 This is a schematic diagram of another angled split structure shown in an embodiment of the present invention; Figure 5 This is a schematic diagram of the main structure of the magnetic stripe shown in an embodiment of the present invention; Figure 6 This is a schematic diagram of the internal structure of the adjustment mounting base shown in an embodiment of the present invention.

[0017] The correspondence between the labels and component names in the attached figures is as follows: 1. Reagent kit body; 2. Sealing cap; 3. Shielding component; 4. First adhesive sheet; 5. Second adhesive sheet; 6. Third adhesive sheet; 7. Fourth adhesive sheet; 8. Positioning block; 9. Sealing gasket; 10. Handheld component; 11. Handle bracket; 12. Adjustable mounting base; 13. Magnetic strip body; 14. Limiting plate; 15. Rack plate; 16. Gear rod; 17. Two-way lead screw. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model. The preferred embodiments of this utility model will now be described in more detail with reference to the accompanying drawings. Although the preferred embodiments of this utility model are shown in the drawings, it should be understood that this utility model can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make this utility model more thorough and complete, and to fully convey the scope of this utility model to those skilled in the art.

[0019] The terminology used in this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular forms “a,” “the,” and “the” used in this invention and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term “and / or” as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0020] It should be understood that although the terms "first," "second," "third," etc., may be used in this invention to describe various information, this information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, without departing from the scope of this invention, first information may also be referred to as second information, and similarly, second information may also be referred to as first information. Thus, features defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0021] Designing a portable magnetic bead-based trace DNA extraction kit is currently the primary technical challenge for engineers.

[0022] To address the aforementioned issues, this invention provides a magnetic bead-based trace DNA extraction kit. This structure allows a handle to be mounted on the kit, enabling multiple kits to be grasped and transported at once by passing a finger through the handle, thus improving the kit's convenience.

[0023] The technical solution of the present invention (Embodiment 1) is described in detail below with reference to the accompanying drawings.

[0024] Figure 1 This is a schematic diagram of the overall structure from one angle shown in one embodiment of this utility model; Figure 2 This is a schematic diagram of the overall structure from another angle, as shown in an embodiment of the present invention; Figure 3 This is a schematic diagram of one of the angle-split structures shown in an embodiment of the present utility model; Figure 4 This is a schematic diagram of another angled split structure shown in an embodiment of the present invention; Figure 5 This is a schematic diagram of the main structure of the magnetic stripe shown in an embodiment of the present invention; Figure 6 This is a schematic diagram of the internal structure of the adjustment mounting base shown in an embodiment of the present invention.

[0025] See Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 The magnetic bead-based trace DNA extraction kit specifically includes: The reagent kit consists of: 1. a main body; 2. a sealing cap; 3. a shielding assembly; 4. a first adhesive sheet; and 5. a second adhesive sheet. The reagent kit body 1 is a rectangular shell structure with an open top. A sealing cap 2 is installed at the top of the reagent kit body 1. A shielding component 3 is symmetrically fixedly connected to the reagent kit body 1. A first adhesive sheet 4 is fixedly connected to the surface of the reagent kit body 1 near the shielding component 3. A second adhesive sheet 5 is symmetrically fixedly connected to the lower surface of the sealing cap 2. The first adhesive sheet 4 and the second adhesive sheet 5 are bonded to each other. A handheld component 10 is fixedly connected to the shielding component 3.

[0026] Specifically, a third adhesive piece 6 is fixedly connected to the lower surface of the shielding component 3, and the third adhesive piece 6 is bonded to the first adhesive piece 4.

[0027] Specifically, a fourth adhesive piece 7 is symmetrically fixedly connected to the upper surface of the sealing cover 2, and the fourth adhesive piece 7 is bonded to the third adhesive piece 6.

[0028] Specifically, a positioning block 8 is provided between the two second adhesive pieces 5 and is fixedly connected to the lower surface of the sealing cap 2. A sealing gasket 9 is fixedly connected to the side of the positioning block 8, and the sealing gasket 9 is attached to the inner wall of the reagent kit body 1.

[0029] Specifically, a handle frame 11 is fixedly connected inside the handheld component 10.

[0030] Specifically, an adjustment mounting base 12 is fixedly connected to the inner wall of the reagent kit body 1, and magnetic strip bodies 13 are equidistantly arranged on the adjustment mounting base 12. Limiting plates 14 are rotatably connected to both sides of the adjustment mounting base 12, and the limiting plates 14 are engaged with the magnetic strip bodies 13.

[0031] Specifically, a rack plate 15 is symmetrically slidably connected inside the adjusting mounting base 12, a gear rod 16 is fixedly connected to the limiting plate 14, the rack plate 15 and the gear rod 16 are meshed together, and a bidirectional lead screw 17 is rotatably connected inside the adjusting mounting base 12, the bidirectional lead screw 17 and the rack plate 15 are meshed together.

[0032] In this embodiment, how to lift multiple reagent kit bodies 1 at once, combined with Figures 1 to 4 The specific implementation method is as follows: the sealing cap 2 is placed close to the reagent kit body 1, the first adhesive sheet 4 and the second adhesive sheet 5 are bonded to each other, the sealing cap 2 is fixedly installed on the top of the reagent kit body 1 to seal and protect the top of the reagent kit body 1, the shielding component 3 is placed close to the reagent kit body 1, the third adhesive sheet 6 and the fourth adhesive sheet 7 are bonded to each other, so that the tester's fingers can pass through multiple handheld components 10 to lift multiple reagent kit bodies 1 at one time, separate the sealing cap 2 from the reagent kit body 1, the third adhesive sheet 6 is bonded to the first adhesive sheet 4, and the shielding component 3 shields and protects the first adhesive sheet 4 to prevent the first adhesive sheet 4 from scratching the tester and affecting the gripping experience.

[0033] In this embodiment, how to position and install the sealing cap 2, combined with Figure 3 and Figure 4 The specific implementation method is as follows: when the sealing cap 2 is close to the reagent kit body 1, the positioning block 8 enters the interior of the reagent kit body 1 for positioning, and the sealing gasket 9 contacts the inner wall of the reagent kit body 1 to fill the gap between the reagent kit body 1 and the sealing cap 2, thereby increasing the sealing effect of the sealing cap 2.

[0034] In this embodiment, how to improve the handheld performance of the handheld component 10, combined with... Figure 3 The specific implementation method is as follows: After the handle 11 is installed inside the hand-held component 10, it can increase the contact area with the fingers, reduce the pressure directly borne by the hand, and the handle 11 can bear greater weight, further improving the hand-held performance of the hand-held component 10.

[0035] In this embodiment, how to simplify the magnetic bead separation effect, combined with Figure 5 and Figure 6 The specific implementation method is as follows: the test cylinder containing magnetic beads is brought close to the adjustment mounting base 12. The magnetic strip body 13 quickly attracts the magnetic beads to the side wall of the cavity under the action of the magnetic field, simplifying the separation steps. The bidirectional lead screw 17 is rotated to drive the rack plates 15 to move closer to each other. The rack plates 15 contact the gear rod 16, which drives the limiting plate 14 to move closer to the magnetic strip body 13 and engage the magnetic strip body 13. The bidirectional lead screw 17 is rotated in the opposite direction to facilitate the rotation and opening of the limiting plate 14, releasing the engaging state of the magnetic strip body 13, and allowing the magnetic strip body 13 to be replaced.

[0036] The present invention has been described in detail above with reference to the accompanying drawings. In the above embodiments, the descriptions of each embodiment have different focuses; for parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments. Those skilled in the art should also understand that the actions and modules involved in the specification are not necessarily essential to the present invention. Furthermore, it is understood that the steps in the method of the present invention embodiments can be adjusted, combined, and deleted according to actual needs, and the structure in the device of the present invention embodiments can be combined, divided, and deleted according to actual needs.

[0037] The various embodiments of the present invention have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A magnetic bead-based trace DNA extraction kit, characterized in that, include: The reagent kit body (1), sealing cap (2), shielding component (3), first adhesive sheet (4), and second adhesive sheet (5); The reagent kit body (1) is a rectangular shell structure with an open top. A sealing cap (2) is installed on the top of the reagent kit body (1). A shielding component (3) is symmetrically fixedly connected to the reagent kit body (1). A first adhesive piece (4) is fixedly connected to the surface of the reagent kit body (1) near the shielding component (3). A second adhesive piece (5) is symmetrically fixedly connected to the lower surface of the sealing cap (2). The first adhesive piece (4) and the second adhesive piece (5) are bonded to each other. A handheld component (10) is fixedly connected to the shielding component (3).

2. The magnetic bead-based trace DNA extraction kit according to claim 1, characterized in that: The lower surface of the shielding component (3) is fixedly connected to a third adhesive piece (6), which is bonded to the first adhesive piece (4).

3. The magnetic bead-based trace DNA extraction kit according to claim 2, characterized in that: The upper surface of the sealing cap (2) is symmetrically fixed with a fourth adhesive piece (7), and the fourth adhesive piece (7) is bonded to the third adhesive piece (6).

4. The magnetic bead-based trace DNA extraction kit according to claim 1, characterized in that: A positioning block (8) is provided between the two second adhesive sheets (5) and is fixedly connected to the lower surface of the sealing cap (2). A sealing gasket (9) is fixedly connected to the side of the positioning block (8) and the sealing gasket (9) is attached to the inner wall of the reagent kit body (1).

5. The magnetic bead-based trace DNA extraction kit according to claim 1, characterized in that: The handheld component (10) has a handle frame (11) fixedly connected inside.

6. The magnetic bead-based trace DNA extraction kit according to claim 4, characterized in that: The inner wall of the reagent kit body (1) is fixedly connected to an adjustment mounting seat (12), and magnetic strip bodies (13) are provided at equal intervals on the adjustment mounting seat (12). Limiting plates (14) are rotatably connected to both sides of the adjustment mounting seat (12), and the limiting plates (14) are engaged with the magnetic strip bodies (13).

7. The magnetic bead-based trace DNA extraction kit according to claim 6, characterized in that: The adjusting mounting base (12) is symmetrically slidably connected to a rack plate (15), and a gear rod (16) is fixedly connected to the limiting plate (14). The rack plate (15) is meshed with the gear rod (16). The adjusting mounting base (12) is rotatably connected to a two-way lead screw (17), and the two-way lead screw (17) is meshed with the rack plate (15).

Images