A bulk transfer device for cryogenic vials

Abstract

The utility model discloses a batch transfer device for cryopreservation tube, including test tube mobile clamp and auxiliary disengagement seat, test tube mobile clamp includes upper movable plate and disengagement board, and is equipped with the pipe chuck and the top pressure subassembly of the cryopreservation tube head pipe cover cooperation on upper movable plate, and the batch fixing and release of cryopreservation tube can be realized through test tube mobile clamp, the auxiliary disengagement seat includes upper base and lower base, and is equipped with the jacking subassembly on lower base, and through auxiliary disengagement seat, not only can assist to improve the fastening effect of test tube mobile clamp batch fixed cryopreservation tube, but also can realize the batch ejection of cryopreservation tube, simultaneously, through the setting of each reset component, the automatic return of each part of the device can be realized. Through the device, the cryopreservation tube in dense arrangement can always keep integral movement in the process of moving between test tube stand and cryopreservation box, improves the moving efficiency, and guarantees the original order of cryopreservation tube, simultaneously, the device simple structure has the advantages of easy operation, high flexibility, low manufacturing cost.

Description

Technical Field

[0001] This utility model belongs to the field of biological experimental auxiliary equipment, and in particular relates to a batch transfer device for cryopreservation tubes. Background Technology

[0002] Successful cryopreservation of biological samples depends on the freezing rate, precise temperature control, and a suitable temperature gradient (+40℃ to -180℃). When the temperature drops to -180℃, most biological samples can be transferred to liquid nitrogen for long-term preservation. During cell cryopreservation, liquid water transforms into ice, and cell metabolism ceases. Simultaneously, water is lost, leading to changes in intracellular salt and metabolite concentrations, resulting in osmotic pressure imbalance and directly affecting the thawing of cryopreserved cells. Furthermore, freezing rates that are too fast or too slow can easily cause intracellular water loss or the formation of large ice crystals, both detrimental to cell survival. By using programmed cooling, selecting appropriate cryopreservation solutions, and setting suitable heating and cooling rates, these adverse factors can be effectively overcome, significantly improving the survival rate of cryopreserved cells. However, the distribution of cryovials directly affects programmed cooling. If the cryovials are arranged too densely, the quality and efficiency of programmed cooling will be affected; if they are arranged too sparsely, it will result in a waste of cryopreservation solution and freezing space. However, in order to save storage space for cryopreservation, cryopreservation boxes are often designed with cryovials tightly packed together. This also means that existing cryopreservation boxes cannot be directly used for programmed cooling. It is necessary to transfer the cryovials, after programmed cooling, to the cryopreservation box before the cryopreservation box is used for cryopreservation.

[0003] Transferring cryovials requires an ultra-low temperature environment, such as liquid nitrogen or liquid nitrogen atmosphere. Therefore, current techniques typically involve placing all cryovials in liquid nitrogen or liquid nitrogen atmosphere before individually placing them onto the cryopreservation box. This is not only extremely inefficient but also can damage the cells within the cryovials due to the prolonged transfer time. Furthermore, operators must be equipped with cryoprotective gear during these routine operations, further increasing the complexity of the procedure. In practice, before using the cryovials, they often need to be moved from the cryopreservation box to a test tube rack. When ultra-low temperature preservation is required, the cryovials must then be moved back to the cryopreservation box. While this is manageable with a small number of cryovials, it becomes extremely labor-intensive when the number is large. Additionally, the placement of the cryovials may be misaligned during the transfer process due to operational errors. Therefore, there is an urgent need for a batch transfer device that can quickly transfer cryopreservation tubes as a whole, so as to realize the batch movement of cryopreservation tubes between cryopreservation boxes and test tube racks. Utility Model Content

[0004] To address the problems existing in the prior art, the purpose of this utility model is to provide a batch transfer device for cryopreserved tubes. Various batch transfer devices can be designed to match different cryopreservation boxes and test tube racks to meet the rapid transfer needs of cryopreserved tubes under different usage environments. Furthermore, this device can utilize simple mechanical motion conversion to achieve batch movement of cryopreserved tubes between test tube racks and cryopreservation boxes, and has an automatic reset function. It is easy to operate, has a simple and compact structure, and is reliable in operation, improving transfer efficiency while maintaining the original order of the cryopreserved tubes.

[0005] To achieve the above and other related objectives, the present invention adopts the following technical solution:

[0006] This utility model provides a batch transfer device for cryopreservation tubes, including a tube moving clamp and an auxiliary detachment seat;

[0007] The test tube moving clamp includes an upper movable plate and a release plate, used for batch fixing and batch release of cryopreservation tubes; the upper movable plate is provided with a plurality of tube clamps, the distribution position of the tube clamps on the upper movable plate is consistent with the placement position of the cryopreservation tubes on the test tube rack, and the lower end of the tube clamp has a shape and size that matches the inner recess of the cryopreservation tube head cap; at the same time, the release plate is provided with a through hole for the tube clamps to pass through, the diameter of the through hole being smaller than the size of the cryopreservation tube head cap;

[0008] The upper movable plate and the release plate are movably connected by a reset component, allowing the release plate to move up and down. A pressing component is hinged to the upper movable plate. The pressing component is used to push the release plate to move away from the upper movable plate. The reset component is used to return the release plate to its initial state, thereby allowing the pipe clamp to reciprocate within the through hole.

[0009] The auxiliary release seat includes an upper base and a lower base; the upper base is provided with a placement platform; the lower base includes a support plate and a base, and a guide plate is clamped and fixed between the support plate and the base; a lifting assembly is movably connected to the support plate, the lifting assembly is used to assist in the batch fixing and batch ejection of cryopreservation tubes, the lifting assembly includes a push rod and a push rod spring, the push rod vertically penetrates the guide plate and the support plate, and is movably connected to the support plate on the lower base through the push rod spring, the distribution position of the push rod on the support plate is consistent with the placement position of the cryopreservation tubes on the test tube rack;

[0010] The upper base and the lower base are movably connected by a reset assembly, allowing the upper base to move up and down. The placement platform, the support plate, and the guide plate are provided with several corresponding through holes, which are used for the top rods in the lifting assembly to pass through when moving up and down.

[0011] As a preferred technical solution, the extrusion assembly includes a first handle and a second handle arranged in a cross manner. The first handle and the second handle are hinged to the upper movable plate at the intersection. The upper ends of the first handle and the second handle both extend upwards from the upper end surface of the upper movable plate, and the lower ends of the first handle and the second handle both abut against the upper end surface of the release plate.

[0012] As a preferred technical solution, the upper movable plate includes a top plate and a clamp mounting plate, and the pipe clamp is fixed to the lower end face of the top plate through the clamp mounting plate.

[0013] As a further preferred technical solution, the top plate is provided with a movable groove for the lower end of the extrusion assembly to pass through; when the top plate has a certain thickness, the extrusion assembly can be directly hinged to the groove wall of the movable groove on the top plate; when the top plate is thin, a positioning block is fixed on the top plate, and the extrusion assembly is fixedly connected to the positioning block by a shaft pin.

[0014] As a preferred technical solution, in the lifting assembly, the longitudinal section of the top rod is T-shaped, including a rod body and a protrusion at the top of the rod body; the top rod spring is sleeved on the top rod body and is located between the lower end face of the top rod and the upper end face of the support plate; the diameter of the top rod spring is larger than the diameter of the through hole on the support plate and smaller than the outer edge dimension of the protrusion at the top of the top rod.

[0015] As a preferred technical solution, the bottom of the push rod is provided with a pin, which passes through the push rod body laterally and is located below the guide plate, in order to prevent the push rod from coming out of the through hole on the guide plate.

[0016] As a preferred technical solution, the reset component is disposed between the upper movable plate and the release plate, and includes a movable clamp guide post and a pressure spring. The movable clamp guide post passes through the upper movable plate and is fixed to the release plate. Furthermore, the movable clamp guide post is movably connected to the upper movable plate through the pressure spring, so that the upper movable plate can move up and down relative to the release plate. The top end of the movable clamp guide post is higher than the upper end surface of the upper movable plate.

[0017] Furthermore, the longitudinal section of the movable clamp guide post is T-shaped, including a post body and a boss at the top of the post body; the pressure spring is fitted onto the post body and is located between the lower end face of the boss and the upper end face of the upper movable plate.

[0018] As a preferred technical solution, the second reset component is disposed between the upper base and the lower base, and includes an auxiliary guide post and a second pressure spring; the auxiliary guide post passes through the guide plate and is fixed on the upper base, and the upper base is movably connected to the guide plate through the second pressure spring, so that the upper base can move up and down relative to the guide plate.

[0019] Furthermore, the longitudinal section of the auxiliary guide column is T-shaped, including a column body two and a boss two disposed at the top of the column body two; the pressure spring two is sleeved on the column body two and is located between the lower end face of the upper base and the upper end face of the guide plate.

[0020] As described above, the present invention has the following beneficial effects:

[0021] (1) The present invention provides a batch transfer device for cryopreservation tubes. Through the cooperation of the test tube moving clamp and the auxiliary release seat, the batch movement of cryopreservation tubes between the test tube rack and the cryopreservation box is realized through simple mechanical motion conversion. Specifically, the tube clamp on the upper movable plate that matches the tube cap recess of the cryopreservation tube head can be used to fix the cryopreservation tubes in batches by interference fit. Furthermore, through the cooperation of the upper movable plate, the release plate and the top pressing component, the top pressing component can be used to push the release plate to move away from the upper movable plate, thereby realizing the batch release of cryopreservation tubes. In addition, through the simultaneous force application of the tube clamp and the lifting component to the head and bottom of the cryopreservation tubes, the fastening effect of the test tube moving clamp in batch fixing of cryopreservation tubes can be improved. At the same time, through the restoring force generated by the lifting component after the relative movement of the upper base and the lower base, the batch ejection of cryopreservation tubes can also be realized.

[0022] (2) The present invention provides a batch transfer device for cryopreservation tubes. Through the tube clamps, through holes on the release plate, top rods and through holes distributed in the same position as the cryopreservation boxes and test tube racks, the cryopreservation tubes can be moved as a whole during the movement. Thus, densely arranged cryopreservation tubes can be moved from the cryopreservation boxes to the test tube racks in a convenient and quick manner, and can also be moved from the test tube racks to the cryopreservation boxes in a convenient and quick manner. It has an automatic reset function, is easy to operate, has a compact structure and is reliable in operation. It not only improves the moving efficiency, but also ensures the original order of the cryopreservation tubes.

[0023] (3) The present invention provides a batch transfer device for cryopreservation tubes. It uses few parts and has a simple structure. It can be made to match different cryopreservation tubes, cryopreservation boxes and test tube racks. With fewer parts and a simple structural design, it can meet the needs of rapid transfer of cryopreservation tubes in different usage environments. For example, the tube clamps with different shapes and sizes at the bottom can be replaced as needed. The upper base of the placement platform with different cross-sectional shapes or different through hole opening positions can also be replaced as needed. Therefore, the device also has the advantages of easy operation, high flexibility and low manufacturing cost. Attached Figure Description

[0024] Figure 1 This is a schematic diagram illustrating the process of batch fixing during the movement of cryopreservation tubes from the test tube rack to the cryopreservation box using a test tube moving clamp in this embodiment.

[0025] Figure 2yes Figure 1 A cross-sectional view at point AA when the cryopreservation tubes are being removed in batches from the test tube rack.

[0026] Figure 3 This is a schematic diagram of moving the cryopreservation tube using the test tube moving clamp in this embodiment.

[0027] Figure 4 This is a schematic diagram of placing cryovials into the cryopreservation box using a test tube moving clamp in this embodiment.

[0028] Figure 5 This is a three-dimensional structural diagram of the test tube moving clamp in this embodiment.

[0029] Figure 6 This is a three-dimensional structural diagram of the auxiliary release seat in this embodiment.

[0030] Figure 7 yes Figure 6 A cross-sectional view of the auxiliary release seat at BB.

[0031] Figure 8 This is a three-dimensional structural diagram of the test tube rack and cryopreservation tubes after the batch transfer is completed in this embodiment.

[0032] Figure 9 This is a three-dimensional structural diagram of the cryopreservation box and cryopreservation tube after the batch transfer is completed in this embodiment.

[0033] The specific annotations in the attached drawings are as follows: 1. Test tube moving clamp; 11. Upper movable plate; 111. Top plate; 112. Tube clamp head; 113. Clamp head mounting plate; 12. Release plate; 13. Reset assembly one; 131. Moving clamp guide post; 132. Pressure spring one; 14. Squeezing assembly; 141. Handle one; 142. Handle two; 143. Shaft pin; 2. Auxiliary release seat; 21. Upper base; 211. Placement platform; 22. Lower base; 221. Support plate; 222. Guide plate; 223. Base; 224. Receiving cavity; 23. Reset assembly two; 231. Auxiliary guide post; 232. Pressure spring two; 24. Lifting assembly; 241. Top rod; 242. Top rod spring; 243. Pin; 3. Cryopreservation tube; 4. Test tube rack; 5. Cryopreservation box. Detailed Implementation

[0034] To better understand the purpose, structure, and function of this utility model, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, and only one implementation method is used as the main line of description, rather than all the embodiments.

[0035] In the description of this utility model, it should be noted that the positional relationships indicated by terms such as "longitudinal," "lateral," "up," "down," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer" are based on the positional relationships shown in the accompanying drawings and are only for the convenience of describing the embodiments of this utility model and simplifying the description. They do not imply that the indicated or referred device or element must have a specific orientation, be constructed or operated in a specific direction, and therefore should not be construed as a limitation of this utility model. Furthermore, when describing some of the main technical features, some alternative solutions to these technical features are interspersed as branches. Since these alternative solutions can be clearly expressed in words, no separate drawings are provided.

[0036] Example

[0037] like Figures 1 to 7 As shown, this embodiment provides a batch transfer device for cryopreservation tubes, including a test tube moving clamp 1 and an auxiliary release seat 2. The test tube moving clamp 1 includes an upper movable plate 11 and a release plate 12 that are movably connected, used for batch fixing and batch release of cryopreservation tubes 3. The auxiliary release seat 2 includes an upper base 21 and a lower base 22 that are movably connected, used to help improve the tightness of the test tube moving clamp in batch fixing of cryopreservation tubes, and at the same time for batch ejection of cryopreservation tubes 3 from the cryopreservation box 5 or the test tube rack 4.

[0038] The upper movable plate 11 includes a top plate 111, a clamp mounting plate 113, and a plurality of pipe clamps 112. The clamp mounting plate 113 is threadedly fixed to the top plate 111. The pipe clamps 112 are mounted and fixed to the lower end face of the top plate 111 via the clamp mounting plate 113. The pipe clamps 112 can rotate around their own central axis to automatically align with the cap of the cryopreservation tube 3. The distribution positions of the pipe clamps 112 on the clamp mounting plate 113 are as follows: The position of the cryopreservation tubes 3 on the test tube rack 4 corresponds to the position of the cryopreservation tubes 3 on the cryopreservation box 5. The lower end of the tube clamp 112 has a shape and size that matches the recess in the tube cap of the cryopreservation tube 3, so that the lower end of the tube clamp 112 can be inserted into the tube cap of the cryopreservation tube 3 and achieve an interference fit. By fixing the lower end of the tube clamp 112 in the recess on the tube cap of the cryopreservation tube 3, the cryopreservation tube 3 can be easily grasped.

[0039] The release plate 12 is used to remove the gripped cryopreservation tube 3 from the test tube moving clamp 1. Specifically, the release plate 12 has a through hole 2 for the tube clamp head 112 to pass through. The distribution position of the through hole 2 is consistent with the distribution position of the tube clamp head 112, and the diameter of the through hole 2 is between the lower end size of the tube clamp head 112 and the tube cap size of the cryopreservation tube 3.

[0040] The upper movable plate 11 and the release plate 12 are movably connected by a reset assembly 13, allowing the release plate 12 to move up and down relative to the upper movable plate 11. A pressing assembly 14 is hinged to the upper movable plate 11, and the lower end of the pressing assembly 14 abuts against the upper end face of the release plate 12. The pressing assembly 14 is used to press the release plate 12 to move it away from the upper movable plate 11, thereby causing the cryopreservation tube 3 to detach from the test tube moving clamp 1. The reset assembly 13 is used to reset the release plate 12. During the process of the release plate 12 returning to its initial state, the tube clamp head 112 can move up and down within the through hole 2 on the release plate 12.

[0041] The extrusion assembly 14 includes a first handle 141 and a second handle 142 arranged in an "X" shape, which are intersected by a pivot pin 143 at their intersection. The upper ends of both the first handle 141 and the second handle 142 extend upwards from the upper surface of the upper movable plate 11, and the lower ends of both the first handle 141 and the second handle 142 abut against the upper surface of the release plate 12. The top plate 111 of the upper movable plate 11 is provided with the first handle 141 and the second handle 142 for use in the extrusion assembly 14. The handle 141 and handle 2142 are mounted on the top plate 111 of the upper movable plate 11 through the movable groove. In this embodiment, when the top plate 111 is thin, a positioning block is provided on the top plate 111. The positioning block is fixed to the side of the movable groove on the top plate 111, and each handle in the extrusion assembly 14 is fixedly connected to the positioning block by a shaft pin 143. In other embodiments, when the top plate 111 has a certain thickness, each handle in the extrusion assembly 14 can be directly hinged to the groove wall of the movable groove on the top plate 111.

[0042] In this embodiment, the reset assembly 13 is disposed between the upper movable plate 11 and the release plate 12, and includes a movable clamp guide post 131 and a pressure spring 132. The upper movable plate 11 has a through hole through which the movable clamp guide post 131 passes. The movable clamp guide post 131 vertically penetrates the upper movable plate 11 through the through hole and is fixed to the release plate 12. The movable clamp guide post 131 is movably connected to the upper movable plate 11 via the pressure spring 132, allowing the upper movable plate 11 to move relative to the release plate 12. The movable clamp guide post 131 moves up and down, with its top end higher than the upper end face of the upper movable plate 11. The longitudinal section of the movable clamp guide post 131 is T-shaped, including a column body and a boss at the top end of the column body. The pressure spring 132 is sleeved on the column body of the movable clamp guide post 131 and is located between the lower end face of the boss on the movable clamp guide post 131 and the upper end face of the upper movable plate. The diameter of the pressure spring 132 is larger than the diameter of the through hole on the upper movable plate and smaller than the diameter of the boss at the top end of the movable clamp guide post 131.

[0043] The upper base 21 is provided with a placement platform 211, which is used to place the cryopreservation box 5 or the test tube rack 4. Preferably, the placement platform 211 has a cross-sectional shape that is adapted to the bottom surface of the cryopreservation box 5 and the test tube rack 4.

[0044] The lower base 22 includes a support plate 221 and a base 223. The support plate 221 is threadedly fixed to the base 223, and a guide plate 222 is clamped and fixed between the support plate 221 and the base 223. A lifting assembly 24 is movably connected to the support plate 221. The lifting assembly 24 is used to eject the cryopreservation tubes 3 in batches from the cryopreservation box 5 or the test tube rack 4. The lifting assembly 24 includes a push rod 241 and a push rod spring 242. The push rod 241 vertically penetrates the guide plate 222 and the support plate 221, and is movably connected to the support plate 221 on the lower base 22 through the push rod spring 242, so that the push rod 241 can move vertically up and down. The distribution position of the top rod 241 on the support plate 221 is consistent with the placement position of the cryopreservation tube 3 on the cryopreservation box 5 and the test tube rack 4; a receiving cavity 224 is provided between the lower base 22, the base 223 and the guide plate 222 for the top rod 241 in the lifting assembly 24 to move up and down.

[0045] The upper base 21 and the lower base 22 are movably connected by a reset assembly 23, allowing the upper base 21 to move up and down relative to the lower base 22. The placement platform 211, the support plate 221, and the guide plate 222 are provided with a number of corresponding through holes. The through holes are used for the top rod 241 in the lifting assembly 24 to pass through when moving up and down. The distribution of the through holes is consistent with the placement position of the cryopreservation tube 3 on the test tube rack 4.

[0046] In this embodiment, the longitudinal section of the push rod 241 is T-shaped, including a rod body and a protrusion at the top of the rod body; the push rod spring 242 is sleeved on the rod body of the push rod 241 and is located between the lower end face of the push rod 241 and the upper end face of the support plate 221; the diameter of the push rod spring 242 is larger than the diameter of the through hole on the support plate 221 and smaller than the diameter of the protrusion at the top of the push rod 241; in a preferred embodiment, a pin 243 is provided at the bottom of the push rod 241, the pin 243 laterally penetrates the rod body of the push rod 241 and is located below the guide plate 222, for preventing the push rod 241 from coming out of the through hole on the guide plate 222.

[0047] The second reset assembly 23 is disposed between the upper base 21 and the lower base 22, and includes an auxiliary guide post 231 and a second pressure spring 232. The auxiliary guide post 231 passes through the guide plate 222 on the lower base 22 and is fixed to the upper base 21. The upper base 21 is movably connected to the guide plate 222 on the lower base 22 through the second pressure spring 232, so that the upper base 21 can move up and down relative to the guide plate 222 on the lower base 22. The longitudinal section of the auxiliary guide post 231 is T-shaped, including a column body and a boss at the top of the column body. The second pressure spring 232 is sleeved on the column body of the auxiliary guide post 231 and is located between the lower end face of the upper base 21 and the upper end face of the guide plate 222 on the lower base 22. The diameters of the second boss and the second pressure spring 232 are both larger than the diameter of the through hole on the guide plate 222.

[0048] The following provides a method for batch moving cryopreservation tubes 3 between test tube rack 4 and cryopreservation box 5 using the above-mentioned batch transfer device, including two usage scenarios: (1) batch moving cryopreservation tubes 3 from test tube rack 4 to cryopreservation box 5; (2) batch moving cryopreservation tubes 3 from cryopreservation box 5 to test tube rack 4.

[0049] (1) The process of transferring cryopreservation tubes 3 in batches from test tube rack 4 to cryopreservation box 5 includes the following steps:

[0050] S1. Secure the cryopreservation tubes 3 on the test tube rack 4 to the test tube moving clamp 1 in batches.

[0051] S1-1. Place the test tube rack 4 in the placement platform 211 on the auxiliary release seat 2, so that the cryopreservation tubes 3 on the test tube rack 4 are aligned with the through holes on the placement platform 211.

[0052] S1-2. Move the test tube moving clamp 1 above the cryopreservation tube 3 so that the tube clamp head 112 on the test tube moving clamp 1 is aligned with the tube cap at the head of the cryopreservation tube 3.

[0053] S1-3. Press down vertically on the handle of the test tube moving clamp 1. By applying downward pressure to the squeezing assembly 14, the downward pressure is transmitted through the test tube moving clamp 1 to the tube clamp head 112, thereby causing the top of the cryopreservation tube 3 to be subjected to a downward force from the tube clamp head 112. At the same time, the downward pressure causes the placement platform 211 and the push rod 241 to move downward, causing the push rod spring 242 on the push rod 241 to compress and generate a restoring force that drives the push rod 241 to move upward. This causes the bottom of the cryopreservation tube 3 on the test tube rack 4 to be subjected to an upward force from the push rod 241. Through the simultaneous force on the head and bottom of the cryopreservation tube 3, the cryopreservation tube 3 and the tube clamp head 112 can be fully locked.

[0054] S1-4. After confirming the locking, release the downward pressure applied to the compression assembly 14. At this time, the placement platform 211 and the push rod 241 return to their initial positions under the restoring force of the pressure spring 232 and the push rod spring 242, respectively; thus completing the batch fixing of the cryopreservation tubes 3.

[0055] S2. Batch detach the cryopreservation tubes 3 from the test tube rack 4: Press down hard on the test tube rack 4 located on the placement platform 211 until the lower end face of the placement platform 211 contacts the upper end face of the support plate 221. At this time, the push rod spring 242 provided on the push rod 241 is compressed and generates a restoring force that drives the push rod 241 to move upward, thereby pushing the cryopreservation tubes 3 out of the test tube rack 4 during the upward movement of the push rod 241.

[0056] S3. Move the cryopreservation tubes 3 in batches to the cryopreservation box 5: By picking up the handle, move the test tube moving clamp 1 above the cryopreservation box 5 so that the cryopreservation tubes 3 are aligned with the placement holes on the cryopreservation box 5 for placing the cryopreservation tubes 3, and put the lower end of the cryopreservation tubes 3 into the corresponding placement holes.

[0057] S4. Remove cryopreservation tubes 3 in batches from test tube moving clamp 1: Hold the handle tightly and apply opposing forces to the squeezing assembly 14. Use the release plate 12 to push the cryopreservation tubes 3 out, so that the lower end of the tube clamp head 112 is disengaged from the recess in the tube cap of the cryopreservation tube 3; complete the movement of cryopreservation tubes 3.

[0058] S4-1. Apply opposing forces to handle 141 and handle 142 in the extrusion assembly 14 to make handle 141 and handle 142 rotate at the hinge point. As they rotate around the pivot pin 143, handle 141 and handle 142 gradually move closer together. At this time, the distance from the hinge point to the release plate 12 becomes relatively longer. The lower ends of handle 141 and handle 142 generate a pushing force on the release plate 12, pushing the release plate 12 to move away from the upper movable plate 11.

[0059] S4-2. Continue to apply opposing forces to handle 141 and handle 142 in the extrusion assembly 14. As the release plate 12 continues to move downward under the pushing force from the lower ends of handle 141 and handle 142 in the extrusion assembly 14, the tube clamp 112 on the upper movable plate 11 moves upward relative to the tube clamp 112 until the lower end face of the tube clamp 112 is higher than the lower end face of the release plate 12. At this time, the head of the cryopreservation tube 3 is squeezed by the lower end face of the release plate 12, thereby realizing the batch separation from the tube clamp 112 and completing the movement of the cryopreservation tube 3.

[0060] At this time, the force applied to the squeezing assembly 14 is removed, and the test tube moving clamp 1 can be restored to its initial state by the restoring force of the reset assembly 13: After the force applied to the handle 141 and handle 142 in the squeezing assembly 14 is removed, the release plate 12 is subjected to the restoring force of the pressure spring 132 in the reset assembly 13, and thus moves towards the upper movable plate 11 until the pressure spring 132 returns to its initial length and the elastic restoring force disappears. At this time, the release plate 12 returns to its initial position, and the lower end face of the tube clamp head 112 is naturally lower than the lower end face of the release plate 12.

[0061] By repeating the above steps, the cryopreserved tubes 3 in another test tube rack 4 can also be moved in batches to the cryopreservation box 5. In this embodiment, after the batch transfer is completed, the placement of the cryopreserved tubes 3 on the cryopreservation box 5 is as follows: Figure 9 As shown.

[0062] (2) The process of transferring the cryopreservation tubes 3 in batches from the cryopreservation box 5 to the test tube rack 4 is basically the same as the process of transferring the cryopreservation tubes 3 in batches from the test tube rack 4 to the cryopreservation box 5 described above, and specifically includes the following steps:

[0063] S1. Secure the cryopreservation tubes 3 on the cryopreservation box 5 to the test tube moving clamp 1 in batches.

[0064] S1-1. Place the cryopreservation box 5 in the placement platform 211 on the auxiliary detachment seat 2, so that the cryopreservation tubes 3 to be transferred in batches on the cryopreservation box 5 are aligned with the through holes on the placement platform 211.

[0065] S1-2. Move the test tube moving clamp 1 above the cryopreservation tube 3 so that the tube clamp head 112 on the test tube moving clamp 1 is aligned with the tube cap at the head of the cryopreservation tube 3.

[0066] S1-3. Press down vertically on the handle of the test tube moving clamp 1. By applying downward pressure to the squeezing assembly 14, the downward pressure is transmitted through the test tube moving clamp 1 to the tube clamp head 112, thereby causing the top of the cryopreservation tube 3 to be subjected to a downward force from the tube clamp head 112. At the same time, the downward pressure causes the placement platform 211 and the push rod 241 to move downward, causing the push rod spring 242 on the push rod 241 to compress and generate a restoring force that drives the push rod 241 to move upward. This causes the bottom of the cryopreservation tube 3 on the cryopreservation box 5 to be subjected to an upward force from the push rod 241. Through the simultaneous force on the head and bottom of the cryopreservation tube 3, the cryopreservation tube 3 and the tube clamp head 112 can be fully locked.

[0067] S1-4. After confirming that the lock is tightened, release the handle. The platform 211 and the top rod 241 will return to their initial positions under the restoring force of the pressure spring 232 and the top rod spring 242, respectively; thus completing the batch fixing of the cryopreservation tubes 3.

[0068] S2. Batch detach cryopreservation tubes 3 from cryopreservation boxes 5: Press down hard on the cryopreservation boxes 5 located on the placement platform 211 until the lower end face of the placement platform 211 contacts the upper end face of the support plate 221. At this time, the push rod spring 242 provided on the push rod 241 is compressed and generates a restoring force that drives the push rod 241 to move upward, thereby pushing the cryopreservation tubes 3 out of the cryopreservation boxes 5 during the upward movement of the push rod 241.

[0069] S3. Move the cryopreservation tubes 3 in batches to the test tube rack 4: By picking up the handle, move the test tube moving clamp 1 above the test tube rack 4 so that the cryopreservation tubes 3 are aligned with the placement holes on the test tube rack 4 for placing the cryopreservation tubes 3, and put the lower end of the cryopreservation tubes 3 into the corresponding placement holes.

[0070] S4. Remove cryopreservation tubes 3 in batches from test tube moving clamp 1: Hold the handle tightly and apply opposing forces to the squeezing assembly 14. Use the release plate 12 to push the cryopreservation tubes 3 out, so that the lower end of the tube clamp head 112 is disengaged from the recess in the tube cap of the cryopreservation tube 3; complete the movement of cryopreservation tubes 3.

[0071] S4-1. Apply opposing forces to handle 141 and handle 142 in the extrusion assembly 14 to make handle 141 and handle 142 rotate at the hinge point. As they rotate around the pivot pin 143, handle 141 and handle 142 gradually move closer together. At this time, the distance from the hinge point to the release plate 12 becomes relatively longer. The lower ends of handle 141 and handle 142 generate a pushing force on the release plate 12, pushing the release plate 12 to move away from the upper movable plate 11.

[0072] S4-2. Continue to apply opposing forces to handle 141 and handle 142 in the extrusion assembly 14. As the release plate 12 continues to move downward under the pushing force from the lower ends of handle 141 and handle 142 in the extrusion assembly 14, the tube clamp 112 on the upper movable plate 11 moves upward relative to the tube clamp 112 until the lower end face of the tube clamp 112 is higher than the lower end face of the release plate 12. At this time, the head of the cryopreservation tube 3 is squeezed by the lower end face of the release plate 12, thereby realizing the batch separation from the tube clamp 112 and completing the movement of the cryopreservation tube 3.

[0073] At this time, the force applied to the squeezing assembly 14 is removed, and the test tube moving clamp 1 can be restored to its initial state by the restoring force of the reset assembly 13: After the force applied to the handle 141 and handle 142 in the squeezing assembly 14 is removed, the release plate 12 is subjected to the restoring force of the pressure spring 132 in the reset assembly 13, and thus moves towards the upper movable plate 11 until the pressure spring 132 returns to its initial length and the elastic restoring force disappears. At this time, the release plate 12 returns to its initial position, and the lower end face of the tube clamp head 112 is naturally lower than the lower end face of the release plate 12.

[0074] Through the above steps, cryopreservation tubes 3 in the cryopreservation box 5 can be moved in batches to any test tube rack 4. In this embodiment, after the batch transfer is completed, the placement of the cryopreservation tubes 3 on the test tube rack 4 is as follows: Figure 8 As shown. When another batch of cryopreservation tubes 3 needs to be transferred, in step S1-1 above, when placing the cryopreservation box 5 on the placement platform 211 on the auxiliary release seat 2, simply rotate the cryopreservation box 5 horizontally by 180° so that each through hole on the placement platform 211 is aligned with the position of the remaining cryopreservation tubes 3 in the cryopreservation box 5. The operation of other steps is the same.

[0075] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art can make various changes or equivalent substitutions to these features and embodiments without departing from the spirit and scope of the present invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are protected by the present invention.

Claims

1. A batch transfer device for cryopreservation tubes, characterized in that, Includes test tube moving clamps and auxiliary release seats; The test tube moving clamp includes an upper movable plate and a release plate, used for batch fixing and batch release of cryopreservation tubes; the upper movable plate is provided with a plurality of tube clamps, the distribution position of the tube clamps on the upper movable plate is consistent with the placement position of the cryopreservation tubes on the test tube rack, and the lower end of the tube clamp has a shape and size that matches the inner recess of the cryopreservation tube head cap; at the same time, the release plate is provided with a through hole for the tube clamps to pass through, the diameter of the through hole being smaller than the size of the cryopreservation tube head cap; The upper movable plate and the release plate are movably connected by a reset component, allowing the release plate to move up and down. A pressing component is hinged to the upper movable plate. The pressing component is used to push the release plate to move away from the upper movable plate. The reset component is used to return the release plate to its initial state, thereby allowing the pipe clamp to reciprocate within the through hole. The auxiliary release seat includes an upper base and a lower base; the upper base is provided with a placement platform; the lower base includes a support plate and a base, and a guide plate is clamped and fixed between the support plate and the base; a lifting assembly is movably connected to the support plate, the lifting assembly is used to assist in the batch fixing and batch ejection of cryopreservation tubes, the lifting assembly includes a push rod and a push rod spring, the push rod vertically penetrates the guide plate and the support plate, and is movably connected to the support plate on the lower base through the push rod spring, the distribution position of the push rod on the support plate is consistent with the placement position of the cryopreservation tubes on the test tube rack; The upper base and the lower base are movably connected by a reset assembly, allowing the upper base to move up and down. The placement platform, the support plate, and the guide plate are provided with several corresponding through holes, which are used for the top rods in the lifting assembly to pass through when moving up and down.

2. The batch transfer device for cryopreservation tubes according to claim 1, characterized in that, The extrusion assembly includes a first handle and a second handle arranged in a cross manner. The first handle and the second handle are hinged to the upper movable plate at the intersection. The upper ends of the first handle and the second handle both extend upwards beyond the upper end surface of the upper movable plate, and the lower ends of the first handle and the second handle both abut against the upper end surface of the release plate.

3. The batch transfer device for cryopreservation tubes according to claim 1, characterized in that, The upper movable plate includes a top plate and a clamp mounting plate, and the pipe clamp is fixed to the lower end face of the top plate through the clamp mounting plate.

4. A batch transfer device for cryopreservation tubes according to claim 3, characterized in that, The top plate has a movable groove for the lower end of the extrusion assembly to pass through; when the top plate has a certain thickness, the extrusion assembly can be directly hinged to the groove wall of the movable groove on the top plate; when the top plate is thin, a positioning block is fixed on the top plate, and the extrusion assembly is fixedly connected to the positioning block by a shaft pin.

5. A batch transfer device for cryopreservation tubes according to claim 1, characterized in that, In the lifting assembly, the longitudinal section of the lifting rod is T-shaped, including a rod body and a protrusion at the top of the rod body; the lifting rod spring is sleeved on the lifting rod body and is located between the lower end face of the lifting rod and the upper end face of the support plate; the diameter of the lifting rod spring is larger than the diameter of the through hole on the support plate and smaller than the outer edge dimension of the protrusion at the top of the lifting rod.

6. A batch transfer device for cryopreservation tubes according to claim 1, characterized in that, The bottom of the push rod is provided with a pin, which passes through the push rod body laterally and is located below the guide plate to prevent the push rod from coming out of the through hole on the guide plate.

7. A batch transfer device for cryopreservation tubes according to claim 1, characterized in that, The reset assembly is disposed between the upper movable plate and the release plate, and includes a movable clamp guide post and a pressure spring. The movable clamp guide post passes through the upper movable plate and is fixed to the release plate. The movable clamp guide post is movably connected to the upper movable plate through the pressure spring, so that the upper movable plate can move up and down relative to the release plate. The top of the movable clamp guide post is higher than the upper end surface of the upper movable plate.

8. A batch transfer device for cryopreservation tubes according to claim 7, characterized in that, The longitudinal section of the movable clamp guide post is T-shaped, including a post body and a boss at the top of the post body; the pressure spring is fitted on the post body and is located between the lower end face of the boss and the upper end face of the upper movable plate.

9. A batch transfer device for cryopreservation tubes according to claim 1, characterized in that, The second reset component is disposed between the upper base and the lower base, and includes an auxiliary guide post and a second pressure spring; the auxiliary guide post passes through the guide plate and is fixed on the upper base, and the upper base is movably connected to the guide plate through the second pressure spring, so that the upper base can move up and down relative to the guide plate.

10. A batch transfer device for cryopreservation tubes according to claim 9, characterized in that, The longitudinal section of the auxiliary guide column is T-shaped, including a column body two and a boss two disposed at the top of the column body two; the pressure spring two is sleeved on the column body two and is located between the lower end face of the upper base and the upper end face of the guide plate.

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