A multi-well assay plate for medical testing

Abstract

The utility model discloses a kind of multi-hole detection plate for medical detection, including plate body, the plate body is equipped with first detection hole cavity and second detection hole cavity for containing test preparation, the first detection hole cavity is enclosed by first containing barrel, first containing barrel bottom has the first barrel bottom plate of flatness, the second detection hole cavity is enclosed by second containing barrel, second containing barrel has the second barrel bottom plate of spherical arc, the bottom of the first containing barrel and second containing barrel is exposed, the first barrel bottom plate and second barrel bottom plate are made of transparent material, the utility model aims at overcoming the deficiency of prior art, provide a kind of multi-hole detection plate for medical detection with stronger adaptability, facilitate detection statistics.

Description

Technical Field

[0001] This utility model specifically relates to a multi-hole detection plate for medical testing. Background Technology

[0002] Multiwell medical testing plates are among the core consumables widely used in biological, chemical, and medical laboratories, playing a crucial role, particularly in enzyme-linked immunosorbent assays (ELISA), cell culture, high-throughput drug screening, molecular diagnostics, and clinical testing. Their basic structure typically consists of a plate with multiple microwells arranged in a standard array to hold reagents or samples. This standardized well placement design enables parallel sample processing and simultaneous detection, significantly improving experimental efficiency, data consistency, and operational repeatability. Taking common 96-well or 120-well assay plates as examples, these products have the following outstanding advantages: First, in terms of efficiency and economy, dozens or even hundreds of samples can be tested simultaneously in a single experiment, significantly reducing the time spent on repeated operations and effectively reducing the consumption of expensive reagents; Second, in terms of accuracy and repeatability, the inner walls of the wells are often treated with surface hydrophilication or coating to ensure uniform liquid spreading, and with high-transparency materials (such as polystyrene, polycarbonate, etc.), they are compatible with various optical detection methods such as absorbance, fluorescence, and luminescence; Third, in terms of data quality optimization, the multi-well layout supports the setting of duplicate control, blank groups, and concentration gradient groups, which helps to improve the statistical reliability and scientific validity of experimental results; Finally, in terms of standardized operation, the uniform shape and well distribution conform to industry standards such as SLAS (Society for Laboratory Automation), and can be seamlessly integrated into high-throughput equipment such as automated pipetting workstations, plate washers, and microplate readers, minimizing errors caused by human intervention.

[0003] However, with the increasing diversification of testing needs, the limitations of traditional multi-well detection plates in terms of their single function have become increasingly apparent. Existing products typically only offer a uniform bottom structure (such as a flat bottom, U-shaped bottom, or V-shaped bottom), making it difficult to simultaneously meet the different testing requirements of quantitative analysis and qualitative observation. For example, flat-bottomed wells are suitable for precise quantitative readings by optical instruments, but lack three-dimensionality when observing cell aggregation or precipitation morphology; while curved-bottomed wells are beneficial for sample aggregation and visual interpretation, they are not conducive to the consistency of bottom imaging. In addition, conventional detection plates tend to form a sealed space when stacked for storage, which prevents residual moisture from evaporating in time after cleaning, posing a risk of microbial contamination; they also lack spill-proof designs and handheld structures during operation, affecting safety and convenience of use.

[0004] This utility model was developed precisely because of the aforementioned shortcomings. Utility Model Content

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a multi-hole detection plate for medical testing that is more adaptable and easier to detect and statistically analyze.

[0006] This utility model is achieved through the following technical solution:

[0007] This utility model provides a multi-hole detection plate for medical testing, comprising a plate body, wherein the plate body is provided with a first detection cavity and a second detection cavity for holding a sample, the first detection cavity is surrounded by a first receiving container, the bottom of the first receiving container has a flat first container bottom plate, the second detection cavity is surrounded by a second receiving container, the second receiving container has a spherical arc-shaped second container bottom plate, the bottoms of both the first and second receiving containers are exposed, and both the first container bottom plate and the second container bottom plate are made of transparent material.

[0008] The porous test plate for medical testing described above, wherein the plate body, the first container, and the second container are an integral structure made of high-transparency plastic material.

[0009] As described above, the multi-well test plate for medical testing has a mating cavity at the bottom of the plate for stacking on top of another plate. The plate has an inner step portion in the mating cavity side, and ventilation openings are provided on opposite side surfaces of the bottom of the plate. When the two plates are stacked, the inner step portion of the upper plate abuts against the top of the lower plate. A distance is maintained between the top of the ventilation opening of the upper plate and the top of the lower plate so that air can be dried on the lower plate through the two ventilation openings.

[0010] As described above, the multi-hole detection plate for medical testing includes a base portion and a boss portion located on the base portion. The boss portion is a shell structure that is raised upward and open at the bottom. The first and second receiving containers are disposed on the boss portion such that the bottoms of both the first and second receiving containers are exposed.

[0011] As described above, in the multi-hole detection plate for medical testing, the boss portion is rectangular and has at least one corner with a chamfer for positioning and orientation.

[0012] As described above, the multi-well detection plate for medical testing has a detection area on the upper surface of the plate for arranging the first and second detection cavities, and a first collection tank and a second collection tank are respectively provided on both sides of the detection area on the upper surface of the plate.

[0013] In the multi-hole detection plate for medical testing described above, the bottoms of both the first and second collection tanks are inclined.

[0014] In the multi-well test plate for medical testing described above, the bottoms of the first and second collection tanks are inclined in the same direction.

[0015] As described above, in the multi-well detection plate for medical testing, the first and second detection cavities are arranged in an array within the detection area, and include at least two rows of first detection cavities and at least five rows of second detection cavities.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] 1. Achieve multifunctional compatible detection and improve experimental efficiency. By integrating a flat-bottomed first detection cavity and a spherical-arc-bottomed second detection cavity on the same plate, it can meet the different needs of quantitative optical detection (such as absorbance and fluorescence reading) and three-dimensional morphological observation (such as cell aggregates and agglutination reactions). Multiple detection modes can be completed without changing consumables. It supports gradient experiments and multiple control groups to be carried out simultaneously, which significantly improves detection efficiency and data comparability.

[0018] 2. The optimized structural design enhances operational safety and environmental adaptability. The stacked structure with ventilation recesses and matching cavities ensures air circulation during stacking, effectively preventing moisture accumulation and microbial growth. At the same time, the liquid collection tank and inclined bottom design collect overflowing liquid and facilitate cleanup, reducing the risk of contamination and ensuring a clean experimental environment and operational safety. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the top surface structure of the multi-hole detection plate of this utility model;

[0020] Figure 2 This is a schematic diagram of the bottom structure of the multi-hole detection plate of this utility model;

[0021] Figure 3 This is a top view of the multi-hole detection plate of this utility model;

[0022] Figure 4 yes Figure 3 A schematic diagram of the cross-section at point AA;

[0023] Figure 5 yes Figure 3 A schematic diagram of the cross-section at point BB;

[0024] Figure 6 This is a schematic diagram of the structure of two multi-hole detection plates stacked together according to this utility model. Detailed Implementation

[0025] The utility model will be further described below with reference to the accompanying drawings:

[0026] The orientations described in this utility model specification, such as "up," "down," "left," "right," "front," and "back," are based on the orientations in the accompanying drawings and are intended to facilitate the description of the relationships between the various components. They do not indicate the unique or absolute positional relationships between the various components, but are merely one embodiment of the utility model and are not a limitation on its implementation.

[0027] This embodiment describes a multi-well detection plate for medical testing, such as... Figures 1 to 5As shown, this porous testing plate includes a plate body 1, on which a first testing cavity 11 and a second testing cavity 12 for holding the test sample are provided. The first testing cavity 11 is surrounded by a first receiving container 13, and the bottom of the first receiving container 13 has a flat first container bottom plate 131. The second testing cavity 12 is surrounded by a second receiving container 14, and the second receiving container 14 has a spherical arc-shaped second container bottom plate 141. The bottoms of both the first receiving container 13 and the second receiving container 14 are exposed. The plate body 1, the first receiving container 13, and the second receiving container 14 are an integral structure made of high-transparency plastic material. The high-transparency plastic material can be selected from polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), transparent polypropylene (TPP), polyethylene terephthalate (PET), etc., so that the bottoms of the first container bottom plate 131 and the second container bottom plate 141 have high transparency. Of course, only the first container bottom plate 131 and the second container bottom plate 141 can be made of high-transparency material. Furthermore, the two measuring cavities have different bottom shapes. The first chamber's bottom plate 131 is resistant to light scattering and provides distortion-free imaging, while the second chamber's bottom plate 141, combined with scattered light, allows for better observation of the three-dimensional morphology and fullness of the sample. These different cavity structures adapt to different optical instruments, facilitating simultaneous gradient detection of different experimental preparations or reagents of varying concentrations. The two different bottom structures meet the needs of various optical detection methods. The first measuring cavity 11 adopts a flat-bottom design (i.e., the first chamber's bottom plate 131 is a planar structure), suitable for quantitative analysis based on transmitted light or confocal microscopy, such as enzyme-linked immunosorbent assay (ELISA), fluorescence reading, and absorbance measurement. Its flat bottom effectively reduces light refraction and scattering at the interface, improving image clarity and signal acquisition consistency, making it particularly suitable for automated plate readers. The second detection well 12 adopts a spherical arc-shaped bottom (i.e., the bottom plate 141 of the second container has an inwardly concave spherical structure), which facilitates the natural convergence of liquid to the lowest point under gravity, improving the recovery rate of trace samples. Simultaneously, the curved surface structure produces a moderate scattering effect on incident light, enhancing the visual contrast of the sample's three-dimensional contour under bright-field or stereomicroscopy, making it easier to observe the three-dimensional morphological characteristics of cell aggregation, precipitate distribution, or agglutination reactions with the naked eye or imaging systems. The combination of these two features constitutes a multifunctional composite detection platform, achieving integrated detection capabilities of "quantitative + qualitative" and "planar imaging + stereoscopic observation." The flat-bottomed well ensures the reliability of high-precision optical detection data; the arc-bottomed well enhances sample visualization, making it particularly suitable for morphological evaluation experiments; multiple detection modes can be operated in parallel on the same plate, saving samples, reagents, and experimental time; and the structure is compatible with mainstream laboratory automation equipment (such as pipetting workstations and ELISA readers).

[0028] As a preferred option, such as Figure 1 and Figure 2As shown, the bottom of the plate 1 has a mating cavity 100 for being stacked on top of another plate 1. The plate 1 has an inner step portion 101 on the side of the mating cavity 100. The bottom of the plate 1 has ventilation openings 15 on opposite side surfaces. Figure 6 As shown, when two boards 1 are stacked, the inner step portion 101 of the upper board 1 abuts against the top of the lower board 1. A distance is maintained between the top of the ventilation recess 15 of the upper board 1 and the top of the lower board 1, allowing air to pass through the two ventilation recesses 15 to dry the lower board 1, preventing microbial contamination during storage. The recess 100 and the inner step portion 101 form a stepped nested structure, ensuring stable alignment of the upper and lower boards during stacking, preventing slippage and improving storage safety. The ventilation recesses 15 are located on both sides of the bottom of the board 1 and retain airflow channels even when stacked, allowing external air to enter the surface area of ​​the lower board from the side, promoting the evaporation of residual moisture and achieving passive ventilation drying. This design cleverly utilizes gravity and natural convection principles to remove moisture without additional energy consumption. It enables safe and stable multi-layer stacking storage, saving space; the ventilation structure effectively reduces the risk of condensation buildup caused by sealed stacking; it reduces conditions for the growth of bacteria and fungi, extending the shelf life or reuse cycle of consumables; it is especially suitable for scenarios where temporary stacking is required after cleaning but before complete drying.

[0029] Detailed structure as follows Figures 1 to 5 As shown, the plate 1 includes a base portion 16 and a boss portion 17 located on the base portion 16. The boss portion 17 is a shell structure that is raised upwards and open at the bottom. The first receiving bucket 13 and the second receiving bucket 14 are disposed on the boss portion 17, so that the bottoms of the first receiving bucket 13 and the second receiving bucket 14 are exposed. As a further optimization, the boss portion 17 is rectangular and has a chamfer 171 at at least one corner for positioning and orientation. This facilitates identification and restriction of the placement direction, and also provides support when multiple plates 1 are stacked. The boss portion 17 adopts a hollow shell design, which improves the overall structural rigidity and reduces weight, in line with the trend of lightweight disposable consumables. The open bottom structure fully exposes the bottom plate 131 of the first bucket and the bottom plate 141 of the second bucket, allowing the light source of the detection equipment to shine vertically from below, avoiding signal attenuation caused by the base obstruction. The 171-degree chamfer serves as a mechanical polarity marker, corresponding to the standard microplate's "top left corner" positioning convention (such as ANSI / SLAS specifications), ensuring users do not misalign the sample during manual loading or automated injection. The exposed bottom design maximizes optical transparency, making it compatible with inverted microscopes and bottom-reading microplate readers; the chamfer provides clear directional guidance, reducing operational error rates; and in automated systems, it can be accurately identified by machine vision, improving compatibility and processing efficiency.

[0030] As a further optimization plan, such as Figures 3 to 5 As shown, the upper surface of the plate 1 is provided with a detection area 2 for arranging the first detection cavity 11 and the second detection cavity 12. The upper surface of the plate 1 has a first collection tank 18 and a second collection tank 19 on both sides of the detection area 2, which facilitates manual operation and collection of leaked liquid. As a further optimization, the bottoms of both the first collection tank 18 and the second collection tank 19 are inclined to facilitate suction via a pipette. Preferably, the inclination direction of the bottoms of the first collection tank 18 and the second collection tank 19 is the same. The first collection tank 18 and the second collection tank 19 are symmetrically distributed along the detection area 2, forming an ergonomic structure similar to a "handle," allowing the experimenter to hold the tank without touching the detection area, preventing contamination. In the event of liquid spillage (such as from pipette misoperation or splashing), the excess liquid will flow into the collection tank and accumulate at the lower point along the inclined bottom, preventing lateral spread to adjacent wells or the instrument platform. The uniform inclination direction facilitates centralized cleaning—simply placing the pipette tip at the lower end allows for one-time suction. Improves ease of operation and handheld stability; effectively isolates contamination sources and protects the cleanliness of the testing area; the inclined tank bottom design improves waste liquid removal efficiency.

[0031] As a preferred embodiment, the first detection well 11 and the second detection well 12 are arranged in an array within the detection area 2, with 10 rows (A-J) arranged horizontally and 12 columns (1-12) arranged vertically. This includes at least two rows and at least five rows of the second detection well 12. The wells are arranged in rows A-J (10 rows) and columns 1-12 (12 columns), conforming to the 96-well plate size specifications (Footprint: 127.76 × 85.48 mm) recommended by the International Organization for Standardization (ISO) and SLAS (International Society for Laboratory Automation), ensuring perfect compatibility with existing pipettes, plate washers, plate readers, and other equipment. Different types of detection wells can be flexibly partitioned according to the experimental design. For example, arc-bottom wells can be concentrated in the central area for focused sample observation, while flat-bottom wells can be distributed around the perimeter for control groups or standard curve determination. Standardized layout ensures equipment compatibility and interchangeability; different types of cavity regions can be customized to support complex experimental schemes; high-throughput screening (HTS) and multi-parameter parallel detection are supported; data recording and software recognition are easy (in conjunction with the LIMS system).

[0032] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A multi-well detection plate for medical testing, characterized in that: The device includes a plate (1), on which a first detection cavity (11) and a second detection cavity (12) for holding the prototype are provided. The first detection cavity (11) is surrounded by a first container (13), and the bottom of the first container (13) has a flat first barrel bottom plate (131). The second detection cavity (12) is surrounded by a second container (14), and the second container (14) has a spherical arc-shaped second barrel bottom plate (141). The bottoms of the first container (13) and the second container (14) are both exposed. The first barrel bottom plate (131) and the second barrel bottom plate (141) are both made of transparent material.

2. The multi-well detection plate for medical testing according to claim 1, characterized in that: The plate (1), the first container (13), and the second container (14) are an integral structure made of high-transparency plastic material.

3. The multi-well detection plate for medical testing according to any one of claims 1 or 2, characterized in that: The bottom of the plate (1) has a mating cavity (100) for being stacked on top of another plate (1). The plate (1) has an inner step (101) on the side of the mating cavity (100). The bottom of the plate (1) has ventilation openings (15) on opposite sides. When the two plates (1) are stacked, the inner step (101) of the upper plate (1) abuts against the top of the lower plate (1). A distance is maintained between the top of the ventilation opening (15) of the upper plate (1) and the top of the lower plate (1) so that air can dry the lower plate (1) through the two ventilation openings (15).

4. The multi-well detection plate for medical testing according to claim 3, characterized in that: The plate (1) includes a base portion (16) and a boss portion (17) located on the base portion (16). The boss portion (17) is a shell structure that is raised upward and open at the bottom. The first container (13) and the second container (14) are disposed on the boss portion (17) such that the bottoms of the first container (13) and the second container (14) are exposed.

5. The multi-well detection plate for medical testing according to claim 4, characterized in that: The boss portion (17) is rectangular and has at least one corner with a chamfer (171) for positioning and orientation.

6. The multi-well detection plate for medical testing according to any one of claims 1 or 2, characterized in that: The upper surface of the plate (1) is provided with a detection area (2) for arranging the first detection cavity (11) and the second detection cavity (12). The upper surface of the plate (1) is provided with a first liquid collection tank (18) and a second liquid collection tank (19) on both sides of the detection area (2).

7. The multi-well detection plate for medical testing according to claim 6, characterized in that: The bottoms of the first liquid collection tank (18) and the second liquid collection tank (19) are both inclined.

8. The multi-well detection plate for medical testing according to claim 7, characterized in that: The bottoms of the first liquid collection tank (18) and the second liquid collection tank (19) are inclined in the same direction.

9. The multi-well detection plate for medical testing according to claim 6, characterized in that: The first detection cavity (11) and the second detection cavity (12) are arranged in an array within the detection area (2), and include at least two rows of first detection cavities (11) and at least five rows of second detection cavities (12).

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