A device for oscillating PCR

Abstract

The utility model discloses a kind of oscillation PCR devices. Oscillation PCR device includes oscillation module and PCR module, PCR module includes temperature control module and hot cover module, oscillation module includes oscillation drive component and oscillation disc, the output end of oscillation drive component is connected with oscillation disc and can drive oscillation disc horizontal oscillation, temperature control module is located on oscillation disc and can be with oscillation disc horizontal oscillation, temperature control module includes temperature control component and refrigeration aperture plate, hot cover module can be set in the upper of refrigeration aperture plate, hot cover module includes heating component. The oscillation PCR device integrates oscillation function, PCR temperature control function and hot cover function, not only make structure small and compact, and can carry out PCR reaction while oscillating, reduce pipetting frequency and reagent loss, reduce the risk of reagent cross-contamination, improve the reliability and consistency of experiment.

Description

Technical Field

[0001] This utility model relates to the field of PCR reaction technology, and in particular to a shaking PCR device. Background Technology

[0002] Next-Generation Sequencing (NGS) technology is an important tool in modern biological research and clinical diagnosis, offering advantages such as the ability to simultaneously detect multiple samples with high resolution. Targeted Next-Generation Sequencing (tNGS) is a type of NGS technology. In pathogen infection diagnosis, tNGS technology is widely used in clinical pathogen detection due to its low data volume, high sensitivity, and ability to accurately and rapidly identify pathogens in infected patients. Besides pathogen diagnosis, NGS also has wide applications in tumor gene detection and genetic variation detection.

[0003] In the rapid development of NGS technology, library preparation is a crucial and complex step, typically involving multiple stages such as DNA fragmentation and PCR amplification or hybridization capture. The cumbersome procedures often lead to operator errors, resulting in library preparation and sequencing failures. Automated pipetting workstations can reduce the risk of experimental failures caused by human factors, improving experimental reproducibility and result consistency. Therefore, automated pipetting workstations are widely used in NGS library preparation and sequencing. Utility Model Content

[0004] The purpose of this invention is to provide a shaking PCR device, which has a high degree of integration, can perform PCR reaction while shaking, requires fewer pipetting operations, has less liquid loss, and has high experimental reliability and consistency.

[0005] To achieve this objective, the present invention adopts the following technical solution:

[0006] A oscillating PCR device includes: an oscillation module comprising an oscillation drive component and an oscillation disk, wherein the output end of the oscillation drive component is connected to the oscillation disk and is capable of driving the oscillation disk to oscillate horizontally; and a PCR module comprising a temperature control module and a heated cover module, wherein the temperature control module is disposed on the oscillation disk and is capable of oscillating horizontally with the oscillation disk, the temperature control module comprising a temperature control component and a cooling well plate disposed on the temperature control component; and a heated cover module being closably disposed above the cooling well plate, the heated cover module comprising a heating component.

[0007] Preferably, the oscillation drive component includes a drive motor, an eccentric sleeve, and a bearing. The eccentric sleeve is sleeved on the motor shaft of the drive motor, and the central axis of the eccentric sleeve is parallel to and spaced apart from the central axis of the motor shaft. The bearing is sleeved outside the eccentric sleeve, and the central axis of the motor shaft is parallel to the central axis of the bearing. The bearing is connected to the oscillation disk.

[0008] Preferably, the drive motor is a brushless DC motor; and / or, the oscillating disk includes a flat plate portion and an annular portion, the flat plate portion is used to support the temperature control module, and the bearing is disposed within the annular portion.

[0009] Preferably, the oscillation module further includes an elastic support structure and a base, the base being spaced below the oscillation disk, one end of the elastic support structure being connected to the base and the other end being connected to the oscillation disk; and / or, the oscillation module further includes a motor base and a support ball, the motor base being disposed between the oscillation drive component and the oscillation disk, and the support ball being rolled between the oscillation disk and the motor base.

[0010] Preferably, there are multiple support balls, which are supported on the bottom surface of the oscillating disk and arranged in a ring.

[0011] Preferably, the cooling perforated plate is used to mount the perforated plate, and the heat cover module has a first state of covering the open end of the perforated plate and a second state of avoiding the perforated plate; and / or, the temperature control module further includes a heat dissipation structure, which is disposed on the side of the temperature control component away from the cooling perforated plate and in contact with the temperature control component.

[0012] Preferably, a first heat-conducting structure is provided between the heat dissipation structure and the temperature control component; and / or, a second heat-conducting structure is provided between the temperature control component and the cooling perforated plate.

[0013] Preferably, the temperature control module further includes a first temperature measuring component, which is disposed at the cooling orifice plate and used to obtain the temperature at the cooling orifice plate; and / or, the temperature control component is a TEC.

[0014] Preferably, a docking mechanism is provided between the temperature control module and the hot cover module. The docking mechanism is used to guide the hot cover module to engage with the temperature control module and limit the movement of the hot cover module.

[0015] Preferably, the docking mechanism includes a docking structure and a plug-in guide post. The docking structure is disposed on the temperature control module and has a docking hole. The plug-in guide post is disposed on the heat cover module and is slidably inserted into the docking hole.

[0016] Preferably, the mating hole is provided with a first magnetic attractor and the insertion guide post is provided with a second magnetic attractor, and the first magnetic attractor and the second magnetic attractor are in an attractive state.

[0017] Preferably, the temperature control module further includes a heat cover shell and a heat-conducting component, the heat cover shell having a mounting cavity, the heat-conducting component being disposed within the mounting cavity, and the heating component being sandwiched between the heat cover shell and the heat-conducting component.

[0018] The beneficial effects of this utility model are:

[0019] This utility model provides a shaking PCR device comprising a shaking module and a PCR module. The PCR module includes a temperature control module and a heated lid module. The shaking module includes a shaking drive component and a shaking plate. The output end of the shaking drive component is connected to the shaking plate and can drive the shaking plate to shake horizontally. The temperature control module is located on the shaking plate and can shake horizontally with the shaking plate. The temperature control module includes a temperature control component and a cooling plate located on the temperature control component. The heated lid module is closable and located above the cooling plate, and includes a heating component. This shaking PCR device integrates shaking, PCR temperature control, and heated lid functions into one unit, making it compact and highly integrated. It also enables PCR reactions to be performed simultaneously with shaking, reducing the number of pipetting operations and reagent loss, lowering the risk of reagent cross-contamination, and improving the reliability and consistency of experiments. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the shaking PCR device provided by this utility model;

[0021] Figure 2 This is a cross-sectional view of the oscillating PCR device provided by this utility model;

[0022] Figure 3 This is a schematic diagram of the oscillation module provided by this utility model;

[0023] Figure 4 This is a cross-sectional view of the oscillation module provided by this utility model;

[0024] Figure 5 This is a schematic diagram of the motor mount provided by this utility model;

[0025] Figure 6 This is an exploded view of the temperature control module provided by this utility model;

[0026] Figure 7 This is a schematic diagram of the heat cover module provided by this utility model;

[0027] Figure 8 This is a cross-sectional view of the heat cover module provided by this utility model.

[0028] In the picture:

[0029] 10. Perforated plate;

[0030] 100. Oscillation module; 110. Oscillation drive component; 111. Drive motor; 112. Eccentric sleeve; 113. Bearing; 120. Oscillation disc; 121. Flat plate; 122. Annular part; 130. Elastic support structure; 140. Base; 150. Motor base; 151. Top plate; 152. Side plate; 153. Annular protrusion; 154. Receiving groove; 160. Support ball; 170. Heat insulation pad;

[0031] 200. Temperature control module; 210. Temperature control component; 220. Cooling perforated plate; 230. Heat dissipation structure; 240. Fixing frame; 250. Connecting structure; 251. Connecting hole;

[0032] 300, Heated cover module; 310, Heating component; 320, Heated cover housing; 330, Heat-conducting component; 331, Metal plate; 332, Silicone pad; 340, Insertion guide post. Detailed Implementation

[0033] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.

[0034] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0035] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0036] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0037] This utility model discloses a shaking PCR device, such as Figures 1 to 8 As shown, the shaking PCR device includes a shaking module 100 and a PCR module. The PCR module includes a temperature control module 200 and a heated cover module 300. The shaking module 100 includes a shaking drive component 110 and a shaking plate 120. The output end of the shaking drive component 110 is connected to the shaking plate 120 and can drive the shaking plate 120 to shake horizontally. The temperature control module 200 is disposed on the shaking plate 120 and can shake horizontally with the shaking plate 120. The temperature control module 200 includes a temperature control component 210 and a cooling plate 220 disposed on the temperature control component 210. The cooling plate 220 is used to mount a multi-well plate 10. The heated cover module 300 is closable and disposed above the cooling plate 220. The heated cover module 300 includes a heating component 310. The heated cover module 300 has a first state where it covers the open end of the multi-well plate 10 and a second state where it avoids the multi-well plate 10. When the heated cover module 300 is in the second state, the multi-well plate 10 can freely enter and exit the cooling plate 220. It should be noted that PCR (Polymerase Chain Reaction) is a technique for rapidly amplifying specific DNA fragments in vitro.

[0038] This invention provides a shaking PCR device that integrates shaking, PCR temperature control, and a heated lid. It performs PCR reactions simultaneously with shaking and precisely controls temperature changes to adapt to the PCR reaction, thereby reducing pipetting operations from the shaker to the PCR instrument in related technologies and minimizing liquid loss caused by simultaneous heat incubation and pipetting in hybridization capture experiments. This integrated instrument, capable of performing PCR reactions while shaking, has significant implications for high-throughput sequencing and molecular diagnostics: First, it significantly improves experimental efficiency by reducing operational steps and time, accelerating library preparation. Second, it reduces the number of sample transfers between different instruments, thus reducing the risk of cross-contamination and improving the reliability and consistency of experimental results. Furthermore, through automated and standardized operation, this integrated instrument improves experimental reproducibility and reduces human error. Finally, it reduces laboratory equipment and maintenance costs, providing researchers with a more economical and efficient solution. In summary, this shaking PCR device is highly integrated, compact, and reduces pipetting and reagent loss, lowering the risk of cross-contamination and improving experimental reliability and consistency.

[0039] Regarding the structure of the oscillation module 100, in some embodiments, refer to... Figure 4 As shown, the oscillation drive component 110 includes a drive motor 111, an eccentric sleeve 112, and a bearing 113. The eccentric sleeve 112 is fitted onto the motor shaft of the drive motor 111, with its central axis parallel to and spaced apart from the central axis of the motor shaft of the drive motor 111. The bearing 113 is fitted outside the eccentric sleeve 112, with its central axis parallel to the central axis of the motor shaft. The bearing 113 is connected to the oscillation disk 120. When the oscillation function is required, the drive motor 111 is powered on, and its motor shaft outputs rotational motion, causing the eccentric sleeve 112 and the bearing 113 to rotate eccentrically together. During the rotation of the bearing 113, the oscillation disk 120 can be pushed to move horizontally in the horizontal plane. It should be noted that, compared with the direct drive method of the motor in related technologies, the oscillation module 100 of this utility model embeds the bearing 113 in the hole of the upper oscillation disk 120. By using the rolling of the bearing 113 to drive the large load (temperature control module 200, perforated plate 10 and heat cover module 300) to move horizontally, the torque of the drive motor 111 can be reduced to the minimum, so as to achieve the effect of a small motor driving a large load.

[0040] Optionally, the drive motor 111 is a brushless DC motor. Brushless DC motors have advantages such as high efficiency, long life, small size, high torque, low maintenance, low noise, and low electromagnetic interference.

[0041] Optionally, the oscillating plate 120 includes a flat plate portion 121 and an annular portion 122. The flat plate portion 121 is used to support the temperature control module 200, and the bearing 113 is disposed in the annular portion 122.

[0042] Continue to refer to Figure 4 and Figure 5 As shown, the oscillation module 100 also includes a motor base 150, a drive motor 111 is mounted on the motor base 150, and the motor base 150 is provided with a through hole through which the motor shaft passes, and the motor shaft protrudes from the motor base 150 after passing through the through hole from top to bottom.

[0043] Optionally, the motor mount 150 includes a generally U-shaped main body, which includes a top plate 151 and two opposing side plates 152. A through hole is provided on the top plate 151, and the drive motor 111 is located between the two side plates 152 and fixed to the top plate 151. The fixing method can be screw connection. The motor mount 150 also includes an annular protrusion 153, which is provided on the top surface of the top plate 151. The annular protrusion 153 forms a space for the annular portion 122 of the oscillating disk 120 to enter, which facilitates assembly and improves structural compactness.

[0044] The oscillation module 100 also includes a base 140, which is spaced below the oscillation disk 120 and supports the motor mount 150. Exemplarily, the side plate 152 of the motor mount 150 is fixedly connected to the base 140, and the fixing method can be screw connection. Optionally, the middle portion of the base 140 has a receiving hole, into which the bottom of the drive motor 111 enters, further enhancing the compactness of the oscillation module 100.

[0045] Continue to refer to Figure 3 and Figure 4 As shown, the oscillation module 100 also includes an elastic support structure 130, one end of which is connected to the base 140, and the other end is connected to the oscillation disk 120. Optionally, the elastic support structure 130 is an elastic column. Optionally, multiple elastic support structures 130 are provided, and the multiple elastic support structures 130 are spaced apart between the oscillation disk 120 and the base 140. For example, four elastic support structures 130 are provided, and the four elastic support structures 130 are arranged in two rows and two columns.

[0046] Continue to refer to Figure 4As shown, the oscillation module 100 also includes support balls 160, which are rolled between the oscillation disk 120 and the motor base 150. Optionally, multiple support balls 160 are provided, and the multiple support balls 160 are rolled and supported on the bottom surface of the oscillation disk 120 in a ring arrangement. The support balls 160 are used to assist in supporting the oscillation disk 120 located above it. In addition, the support function of the support balls 160 can also be supported by a planar thrust bearing. Optionally, the support balls 160 are metal balls, and for example, the support balls 160 are steel balls. Optionally, multiple receiving grooves 154 are provided on the top of the annular protrusion 153 of the motor base 150, and each receiving groove 154 contains a support ball 160. The number of support balls 160 can be flexibly set as needed. For example, there are eight support balls 160, and the eight support balls 160 are evenly arranged on the annular protrusion 153.

[0047] Continue to refer to Figure 3 and Figure 4 As shown, the oscillation module 100 also includes a heat insulation pad 170, which is disposed on the support surface of the oscillation disk 120. The heat insulation pad 170 is located between the oscillation disk 120 and the temperature control module 200 to reduce heat transfer, thereby ensuring the working stability and working accuracy of the oscillation module 100.

[0048] The cooling perforated plate 220 is a plate-like structure with multiple holes. The tubes of the perforated plate 10 can be inserted into the holes of the cooling perforated plate 220 one by one, thereby achieving positioning and assembly. It should be noted that the perforated plate 10 can be a 96-hole plate, such as a 96-hole aluminum plate or a 96-hole plastic plate. Of course, in addition to 96-hole plates, other specifications of perforated plates 10 can also be used. The cooling perforated plate 220 is compatible with the perforated plate 10, and the cooling perforated plate 220 can be replaced according to the perforated plate 10.

[0049] In some embodiments, the temperature control component 210 is a TEC (thermal refrigeration device). A TEC, also known as a semiconductor cooler, is a solid-state refrigeration technology based on the Peltier effect, which achieves precise temperature control by driving semiconductor materials with an electric current. Of course, besides using a TEC as the temperature control component 210, other methods can also be used to achieve the cooling effect, such as absorption refrigeration and magnetic refrigeration. The number of TECs can be flexibly set according to requirements; for example, four TECs are provided.

[0050] Continue to refer to Figure 6 As shown, the temperature control module 200 also includes a fixing frame 240, in which a mounting groove is formed, and the TEC is fixed in the mounting groove. Optionally, if the TEC is a rectangular plate structure, the fixing frame 240 is a square frame. Optionally, a separator for separating multiple TECs is also provided in the fixing frame 240.

[0051] To facilitate the positioning of the cooling perforated plate 220, the fixing frame 240 also has a limiting groove, in which the bottom of the cooling perforated plate 220 is placed. It should be noted that the mounting groove and the limiting groove can be connected to form a stepped hole.

[0052] Continue to refer to Figure 6 As shown, the temperature control module 200 also includes a heat dissipation structure 230, which is located on the side of the temperature control component 210 away from the cooling perforated plate 220 and is in contact with the temperature control component 210. Optionally, the heat dissipation structure 230 is a heat sink, such as a heat sink fin. Of course, in addition to heat sink fins, other heat dissipation components, such as a cooling fan, can also be used.

[0053] To accelerate heat transfer between the temperature control component 210 and the cooling perforated plate 220, and between the heat dissipation structure 230 and the temperature control component 210, and to achieve better heat transfer, in some embodiments, a first thermally conductive structure is provided between the heat dissipation structure 230 and the temperature control component 210; a second thermally conductive structure is provided between the temperature control component 210 and the cooling perforated plate 220. Optionally, the first thermally conductive structure is thermally conductive silicone grease; the second thermally conductive structure is also thermally conductive silicone grease. Of course, in addition to thermally conductive silicone grease, other structures with good thermal conductivity can also be used, such as the metal thermally conductive component 330.

[0054] To precisely control the temperature of the reaction environment of the multi-well plate 10 within the cooling plate 220, the temperature control module 200 further includes a first temperature sensing component. This first temperature sensing component is located at the cooling plate 220 and is used to acquire the temperature at that location. Optionally, the first temperature sensing component is a temperature sensor. When an external electrical signal controls the TEC to heat or cool, the temperature of the cooling plate 220 located above it changes accordingly. Through precise control by the temperature sensor, the temperature conditions required for the PCR reaction can be achieved.

[0055] The heated lid module 300 is designed to prevent reagent evaporation and cross-contamination during PCR reactions. The heated lid module 300 has two states: a first state and a second state. To enable the heated lid module 300 to quickly and accurately switch from the second state to the first state, refer to... Figure 6 and Figure 7 The temperature control module 200 includes a docking mechanism for guiding the hot cover module 300 to engage with the temperature control module 200 and limiting the movement of the hot cover module 300.

[0056] In some embodiments, the docking mechanism includes a docking structure 250 and a insertion guide post 340. The docking structure 250 is disposed on the temperature control module 200 and has a docking hole 251. The heat cover module 300 includes the insertion guide post 340, which is slidably inserted into the docking hole 251. It should be noted that the docking structure 250 can be integrated with the heat dissipation structure 230. The docking hole 251 and the insertion guide post 340 can be configured in multiple sets as needed. For example, there are four docking holes 251 and four insertion guide posts 340, and the four insertion guide posts 340 are inserted into the docking holes 251 one by one to achieve the insertion fit.

[0057] To improve the stability of the hot cover module 300 in the second state, in some embodiments, a first magnetic attractor is provided in the mating hole 251, and a second magnetic attractor is provided on the insertion guide post 340. Optionally, the insertion guide post 340 is a hollow structure, and the second magnetic attractor is located inside the insertion guide post 340. The first and second magnetic attractors are in an attracted state. Optionally, the first magnetic attractor is an electromagnet, and the second magnetic attractor is a ferromagnetic metal part. When the electromagnet is energized, it can attract the ferromagnetic metal part. When the electromagnet is de-energized, it can release the attraction to the ferromagnetic metal part, thereby allowing the hot cover module 300 to switch to the second state. It should be noted that the insertion guide post 340 has two functions: first, to position the hot cover module 300 and the temperature control module 200; second, when the electromagnet in the temperature control module 200 is energized, it can attract the hot cover module 300, so that the hot cover module 300 is fixed and presses the perforated plate 10.

[0058] Continue to refer to Figure 8 As shown, the temperature control module 200 also includes a heat-conducting shell 320 and a heat-conducting element 330. The heat-conducting shell 320 serves to insulate and maintain the appearance, while the heat-conducting element 330 serves to conduct heat. The heat-conducting shell 320 has a mounting cavity, and the heat-conducting element 330 is disposed within the mounting cavity. The heating element 310 is sandwiched between the heat-conducting shell 320 and the heat-conducting element 330. Optionally, the heating element 310 is an electric heating element, such as a metal heating element or a PTC heating element.

[0059] Optionally, the heat-conducting component 330 includes a metal plate 331 and a silicone pad 332. The top surface of the metal plate 331 is provided with a first receiving groove, and the heating component 310 is a heating element disposed in the receiving groove. The bottom surface of the metal plate 331 is provided with a second receiving groove, and the silicone pad 332 is disposed in the second receiving groove. In some embodiments, the insertion guide post 340 and the metal plate 331 are fixedly connected by screws.

[0060] The temperature control module 200 also includes a second temperature measuring component, which is located at the metal plate 331 and is used to obtain the temperature at the heating component 310. Optionally, the second temperature measuring component is a temperature sensor.

[0061] The oscillating PCR device also includes a control mechanism, which can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a combination of multiple distributed microcontrollers. The microcontroller can run a control program to control all electrical control components such as the drive motor 111, temperature control component 210, and heating component 310 to perform their respective functions.

[0062] The following describes the working process of a shaking PCR device:

[0063] Once the entire oscillating PCR apparatus is ready, firstly, switch the heated lid module 300 to the second state and place the multiwell plate 10 containing the biochemical reaction materials into the cooling plate 220; then, switch the heated lid module 300 to the first state, specifically by inserting the insertion guide post 340 into the docking hole 251, energizing the electromagnet to attract the ferromagnetic metal part, thus firmly attaching the heated lid module 300 to the temperature control module 200; next, after the drive motor 111 rotates at the set speed, the motor shaft of the drive motor 111 drives the eccentric sleeve 112 and bearing 113 connected above to rotate eccentrically. During the movement of the bearing 113, the push... The oscillating plate 120 moves in a horizontal plane. With the support and damping effect of four elastic columns and the auxiliary support of circumferentially distributed steel balls, the oscillating plate 120 oscillates periodically in the horizontal plane. The temperature control module 200 is fixed on the oscillating plate 120, so that the entire temperature control module 200 and the heat cover module 300 oscillate together with the oscillating plate 120, thereby realizing the simultaneous oscillation and PCR reaction of the biochemical reaction materials in the multi-well plate 10. Finally, after the reaction is completed, the electromagnet is de-energized, the heat cover module 300 is removed, and the multi-well plate 10 containing the reagent materials is removed for subsequent testing, thus completing the entire oscillation and PCR process.

[0064] In summary, the oscillating PCR device disclosed in this invention integrates oscillation, PCR temperature control, and heated lid functions into one unit. It not only possesses the functions of a traditional shaker but also combines the functions of a PCR instrument and a heated lid. Compared to traditional library preparation, which typically involves shaking biological samples with a shaker followed by PCR amplification using two different devices, this invention requires only one small device to complete the high-load oscillation, temperature control module 200, multi-well plate 10, heated lid module 300, and 96-well PCR amplification of biological samples. Furthermore, the use of an indirect horizontal rolling drive motor 111 instead of a direct drive enables high-load oscillation driven by a small motor; the heated lid module 300 design reduces experimental time and the risk of reagent cross-contamination; and the modular design integrating the oscillation module 100, temperature control module 200, and heated lid module 300 results in a compact and small structure, facilitating integration into pipetting workstations, enabling automated production, saving experimental time, and improving production efficiency.

[0065] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A shaking PCR device, characterized in that, include: An oscillation module (100) includes an oscillation drive component (110) and an oscillation disk (120). The output end of the oscillation drive component (110) is connected to the oscillation disk (120) and can drive the oscillation disk (120) to oscillate horizontally. The PCR module includes a temperature control module (200) and a heated cover module (300). The temperature control module (200) is disposed on the oscillating plate (120) and can oscillate horizontally with the oscillating plate (120). The temperature control module (200) includes a temperature control component (210) and a cooling perforated plate (220) disposed on the temperature control component (210). The heated cover module (300) is disposed above the cooling perforated plate (220) and can be opened and closed. The heated cover module (300) includes a heating component (310).

2. The shaking PCR device according to claim 1, characterized in that, The oscillation drive component (110) includes a drive motor (111), an eccentric sleeve (112), and a bearing (113). The eccentric sleeve (112) is sleeved on the motor shaft of the drive motor (111). The central axis of the eccentric sleeve (112) is parallel to and spaced apart from the central axis of the motor shaft. The bearing (113) is sleeved outside the eccentric sleeve (112), and the central axis of the motor shaft is parallel to the central axis of the bearing (113). The bearing (113) is connected to the oscillation disk (120).

3. The shaking PCR device according to claim 2, characterized in that, The drive motor (111) is a DC brushless motor; And / or, the oscillating disk (120) includes a flat plate portion (121) and an annular portion (122), the flat plate portion (121) is used to support the temperature control module (200), and the bearing (113) is disposed in the annular portion (122).

4. The shaking PCR device according to claim 2, characterized in that, The oscillation module (100) further includes an elastic support structure (130) and a base (140). The base (140) is spaced below the oscillation disk (120). One end of the elastic support structure (130) is connected to the base (140), and the other end is connected to the oscillation disk (120). And / or, the oscillation module (100) further includes a motor base (150) and a support ball (160), the motor base (150) being disposed between the oscillation drive component (110) and the oscillation disk (120), and the support ball (160) being rotatably disposed between the oscillation disk (120) and the motor base (150).

5. The shaking PCR device according to claim 4, characterized in that, Multiple support balls (160) are provided, and the multiple support balls (160) are supported on the bottom surface of the oscillating disk (120) and arranged in a ring.

6. The shaking PCR device according to claim 1, characterized in that, The cooling perforated plate (220) is used to install the perforated plate (10), and the heat cover module (300) has a first state of covering the opening end of the perforated plate (10) and a second state of avoiding the perforated plate (10). And / or, the temperature control module (200) further includes a heat dissipation structure (230), which is disposed on the side of the temperature control component (210) away from the cooling perforated plate (220) and in contact with the temperature control component (210).

7. The shaking PCR device according to claim 1, characterized in that, The temperature control module (200) further includes a first temperature measuring component, which is disposed at the cooling orifice plate (220) and is used to obtain the temperature at the cooling orifice plate (220); And / or, the temperature control component (210) is a TEC.

8. The shaking PCR device according to claim 1, characterized in that, A docking mechanism is provided between the temperature control module (200) and the heat cover module (300). The docking mechanism is used to guide the heat cover module (300) to engage with the temperature control module (200) and limit the movement of the heat cover module (300).

9. The shaking PCR device according to claim 8, characterized in that, The docking mechanism includes a docking structure (250) and a plug-in guide post (340). The docking structure (250) is disposed on the temperature control module (200) and has a docking hole (251). The plug-in guide post (340) is disposed on the heat cover module (300) and is slidably inserted into the docking hole (251).

10. The shaking PCR device according to claim 9, characterized in that, The docking hole (251) is provided with a first magnetic attractor, and the insertion guide post (340) is provided with a second magnetic attractor. The first magnetic attractor and the second magnetic attractor are in an attractive state.

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