Shaped assay detection chip for multiple sample containment cavities

Abstract

The formed component detection chip of the multi-sample containing cavity comprises at least four detection channels; each detection channel comprises a sample containing cavity, a sample adding port and an exhaust port; the sample containing cavity is used for containing a liquid sample; the sample adding port is communicated with the sample containing cavity, and the sample adding port is used for adding the liquid sample to the sample containing cavity; the exhaust port is communicated with the sample containing cavity; the height H of the microscopic imaging area of the sample containing cavity ranges from 0.1 mm to 1.0 mm; the area S of the microscopic imaging area of a single sample containing cavity is greater than or equal to 150 mm2. Or the formed component detection chip comprises two detection channels, wherein the microscopic imaging area of the sample containing cavity of one detection channel is a long and narrow rectangle, the width of the microscopic imaging area is less than the width of the microscopic imaging area of the sample containing cavity of the other detection channel. Or the formed component detection chip comprises two detection channels, and the total area of the microscopic imaging area is greater than or equal to 300 mm2.

Description

Technical Field

[0001] This application belongs to the field of detection technology, and specifically relates to a chip for formed element analysis with multiple carrier channels. Background Technology

[0002] In existing technologies, analytical chips used for microscopic imaging typically have only one analytical detection channel, making it impossible to analyze and detect two different samples simultaneously. However, in practical applications, situations frequently arise where different samples need to be detected simultaneously, such as blood and feces being examined under a microscope at the same time. To analyze and detect two different samples, chip switching is required, reducing efficiency.

[0003] In existing technologies, analytical chips with single analytical detection channels cannot simultaneously handle similar samples with specific concentration relationships. In practical applications, it is common to encounter situations where the same sample has been diluted at different ratios or treated using different methods, and both require microscopic magnification. For such comparative microscopy, analytical chips with single analytical detection channels not only have low imaging efficiency but also reduce the consistency of subsequent comparisons. Comparisons can be performed on the same chip, making it easier to ensure the consistency of experimental conditions and improving the efficiency of comparative analysis. How to provide multiple channels for detecting different samples is one of the technical problems this application aims to solve.

[0004] In existing technologies, when performing microscopic examination on formed elements of different concentrations in the same sample, the detection of some low-concentration formed elements requires a larger sample volume. When detecting concentration using microscopic imaging, the liquid sample needs to be spread out to allow the formed elements to be evenly distributed before concentration detection can be performed based on the image. If the cavity containing a single sample is too large, air bubbles are easily generated during the spreading process of the liquid sample, affecting microscopic imaging, and the spreading process takes longer.

[0005] One of the technical problems this application aims to solve is how to enable samples to quickly expand to a sufficiently large area to meet the requirements of formed element detection. Summary of the Invention

[0006] The detection channel includes a sample receiving cavity. Microscopic imaging requires that the height of the sample receiving cavity cannot be too high, generally less than 1 mm. Through long-term use and experience, the applicant has found that when the chip is placed horizontally, it takes a relatively long time for the sample to flow and distribute properly after addition; therefore, the length of the sample receiving cavity cannot be too long. At the same time, if the width of the sample receiving cavity is too wide, cavities are easily formed during flow. Therefore, the length and width of the sample receiving cavity must be designed within an appropriate range. However, many detection projects require sufficient sample capacity, necessitating a certain size for the sample receiving cavity.

[0007] To avoid the shortcomings of existing technologies, such as the inability to provide multiple analytical detection channels for analytical chips or the long time required for large-area sample unfolding and the potential for air bubbles, a multi-channel analytical chip with two or more analytical detection spaces is provided. This allows the sample to quickly unfold to a sufficiently large area when being tested on the same sample, and can also be used to support different samples to be microscopically imaged, thereby improving detection efficiency.

[0008] A formed element detection chip with a multi-sample containment cavity includes at least four detection channels; each detection channel includes a sample containment cavity, a sample inlet, and an exhaust port; the sample containment cavity is used to contain liquid samples; the sample containment cavity includes a microscopic imaging region; the sample inlet is connected to the sample containment cavity and is used to add liquid samples to the sample containment cavity; the exhaust port is connected to the sample containment cavity; the height H of the microscopic imaging region of the sample containment cavity is greater than 0.1 mm and less than 1.0 mm; the area S of the microscopic imaging region of the sample containment cavity is greater than 150 square millimeters.

[0009] It can be that the detection channel includes detection channel A and detection channel B; the height of the microscopic imaging area of ​​the sample containing cavity of detection channel A and detection channel B is the same or different; the area of ​​the microscopic imaging area of ​​the sample containing cavity of detection channel A and detection channel B is the same or different; and the area S of the microscopic imaging area of ​​the sample containing cavity is less than 500 square millimeters.

[0010] It can be that the detection channels include detection channel A, detection channel B, detection channel C, and detection channel D; the area of ​​the microscopic imaging region of the sample receiving cavity of detection channel A is AS, and the height is AH; the area of ​​the microscopic imaging region of the sample receiving cavity of detection channel B is BS, and the height is BH; the area of ​​the microscopic imaging region of the sample receiving cavity of detection channel C is CS, and the height is CH; AS, BS, and CS are not equal, and / or AH, BH, and CH are not equal.

[0011] A formed element detection chip includes at least two detection channels, an A detection channel and an E detection channel. The A detection channel includes an A sample receiving cavity, an A sample application port, and an A vent port. The A sample application port is connected to the A sample receiving cavity and is used to add a liquid sample to the A sample receiving cavity. The A vent port is also connected to the A sample receiving cavity. The E detection channel includes an E sample receiving cavity, an E sample application port, and an E vent port. The E sample application port is connected to the E sample receiving cavity and is used to add a liquid sample to the E sample receiving cavity. The E vent port is also connected to the E sample receiving cavity. The width of the microscopic imaging area of ​​the E detection channel sample receiving cavity is smaller than the width of the A detection channel sample receiving cavity.

[0012] The range of the height H of the microscopic imaging region of the cavity containing sample A is: greater than 0.1 mm and less than 1.0 mm; the area S of the microscopic imaging region of the cavity containing sample A is greater than 150 square millimeters; the range of the height H of the microscopic imaging region of the cavity containing sample E is: greater than 0.1 mm and less than 1.0 mm; the area of ​​the microscopic imaging region of the cavity containing sample E is greater than 20 square millimeters and less than 60 square millimeters.

[0013] Yes, the width of the cavity containing the E sample is greater than the width of a microscopic image.

[0014] A formed element detection chip with multiple sample containment cavities includes at least one detection channel; the detection channel includes at least two sample containment cavities, at least one sample application port, and at least two exhaust ports; the sample containment cavities include sample containment cavity A and sample containment cavity B; the sample application port includes sample application port A; the exhaust ports include exhaust port A and exhaust port B; sample containment cavity A and sample containment cavity B are used to contain liquid samples; sample application port A is connected to sample containment cavity A and is used to add liquid samples to sample containment cavity A; exhaust port A is connected to sample containment cavity A; sample application port A is connected to sample containment cavity B and is used to add liquid samples to sample containment cavity B; exhaust port B is connected to sample containment cavity B; the height H of the microscopic imaging region in sample containment cavity A and sample containment cavity B is greater than 0.1 mm and less than 1.0 mm; sample containment cavity A includes microscopic imaging region A; sample containment cavity B includes microscopic imaging region B.

[0015] It is possible that the sum of the areas of microscopic imaging region A and microscopic imaging region B is greater than 300 square millimeters.

[0016] It may also include a sample receiving cavity C and an exhaust port C; a sample application port A is connected to the sample receiving cavity C and is used to add liquid samples to the sample receiving cavity C; an exhaust port C is connected to the sample receiving cavity C; and / or may also include a sample receiving cavity D and an exhaust port D; a sample application port A is connected to the sample receiving cavity D and is used to add liquid samples to the sample receiving cavity D; an exhaust port D is connected to the sample receiving cavity D.

[0017] It is possible to centrally locate the sample loading ports at the center of the chip, and to locate the exhaust ports away from the center of the chip.

[0018] Yes, the sample inlet can be semi-circular or elliptical.

[0019] Yes, it is possible if the area of ​​the sample inlet is larger than that of the exhaust port.

[0020] It can include layers K1, K2, and K3; layer K2 is located between layers K1 and K3, layer K2 includes a hollowed-out middle layer, and layer K1 includes a hollowed-out upper sample loading port and a hollowed-out upper exhaust port; after layers K1, K2, and K3 are bonded together, the hollowed-out middle layer is enclosed by layers K1 and K3 to form a sample receiving cavity; the hollowed-out upper sample loading port is connected to the sample receiving cavity to form a sample loading port; the hollowed-out upper exhaust port is connected to the sample receiving cavity to form an exhaust port.

[0021] Yes, the thickness D of the K2 layer can be greater than 0.1 mm and less than 1.0 mm.

[0022] It is possible that layer K1 or layer K3 is made of transparent glass or plastic, and layer K2 is made of glass; a recessed space is provided on layer K1, and the recessed space on layer K1 and layer K2 enclose to form a detection channel; or a protrusion is provided on layer K2, and the protrusions on layers K1 and K2 enclose to form a detection channel.

[0023] It can include E1 layer and E2 layer; E1 layer and E2 layer are relatively bonded together; E1 layer includes a sample inlet cutout and an exhaust port cutout; the bonding surface of E1 layer or E2 layer includes a groove; after E1 layer and E2 layer are bonded together, the groove is surrounded by E1 layer and E2 layer to form a sample receiving cavity; the sample inlet cutout is connected to the sample receiving cavity to form a sample inlet; the exhaust port cutout is connected to the sample receiving cavity to form an exhaust port.

[0024] It is permissible that the depth of at least one groove is different from the depth of the other grooves.

[0025] One of the technical advantages of the above-mentioned technical solution is that the analysis chip with multiple analytical detection channels has a sufficiently large microscopic imaging area in the single sample-containing cavity, which can at least meet the concentration detection of one type of formed element, such as the detection of white blood cell content in blood; the single sample-containing cavity is larger than 150 square millimeters, which meets the detection volume requirement of a single sample.

[0026] One of the technical advantages of the above-mentioned technical solution is that, while ensuring that the single sample accommodating cavity is larger than 150 square millimeters, four channels can be set on one chip. This allows for the setting of multiple detection channels within a reasonable chip size, enabling one chip to support the detection of multiple items or multiple samples.

[0027] One of the technical advantages of the above solution is that the single sample-containing cavity is less than 500 square millimeters, preventing cavitation during sample addition and reducing the sample addition and expansion time. Excessive sample addition and expansion time leads to uneven distribution of the detection target in the sample; longer channels result in lower target content in subsequent samples.

[0028] One of the technical advantages of the above-mentioned technical solution is that the four channels facilitate the injection of more different samples.

[0029] One of the technical advantages of the above-mentioned solution is that the microscopic imaging areas of the two sample-containing cavities can be the same or different, allowing for the application of different samples for microscopic imaging; the tiling area, i.e., the microscopic imaging area of ​​different liquid samples, can be flexibly adjusted. For example, when identifying different target objects, the appropriate sample volume can be selected based on the approximate concentration range of the target object, and injected into the corresponding detection channel to obtain the optimal tiling area, thereby improving the efficiency of imaging and subsequent image processing.

[0030] One of the technical effects of the above-mentioned technical solution is that the setting of the height and area of ​​the microscopic imaging region can be applied to a variety of different types of samples.

[0031] One of the technical advantages of the above-mentioned solution is that the sample-containing cavities of the two detection channels have different heights, which facilitates the combination with the area of ​​the microscopic imaging region to form different sample capacities, allowing for more flexible injection of different sample capacities. When a sample contains multiple formed elements, the content of different formed elements may vary greatly. Therefore, the volume of the target imaging sample can be adjusted by adjusting the sample volume, ensuring that the distribution of formed elements in a tiled field of view is of a suitable quantity for observation.

[0032] For example, the concentration of white blood cells in blood is relatively lower than that of red blood cells. During blood testing, both red blood cell and white blood cell concentrations need to be measured. Using traditional methods, to simultaneously detect and identify both red and white blood cells, when red blood cells are laid flat, the number of white blood cells in a single microscopic field of view is very low, sometimes even nonexistent. Multiple images need to be combined to create a larger sample volume for effective identification and calculation of white blood cell concentration, thus wasting significant imaging resources. With the combination of different channel areas and heights described in this application, red blood cells and white blood cells can be imaged and identified separately in different channels. For example, for red blood cell identification, the sample size is based on the flat imaging of red blood cells in one field of view. For white blood cell identification, the sample size is based on the flat imaging of white blood cells in one field of view. For different formed elements, the sample size can be flexibly adjusted according to their concentration range.

[0033] One of the technical advantages of the above-mentioned solution is that the height and area dimensions can be adjusted, further expanding the range of sample volume and improving adaptability.

[0034] One of the technical advantages of the above-mentioned solution is that the width of the microscopic imaging area of ​​the sample receiving cavity in the E detection channel is smaller than that in the A detection channel; this facilitates sample volume adjustment and differentiation; the narrow microscopic imaging area facilitates microscopic imaging, allowing movement in only one direction and reducing control complexity. The different fluid adaptability of the E and A detection channels makes them suitable for the simultaneous detection of samples with different properties.

[0035] One of the technical advantages of the above-mentioned technical solution is that the width of the sample-containing cavity of the E-detection channel is greater than the width of a microscopic imaging image. During the microscopic imaging process, the image can be completed by moving the relative position of the microscope once or multiple times.

[0036] One of the technical advantages of the above-mentioned solution is that the microscopic imaging areas of the two channels are different, which facilitates the combination of different sample sizes.

[0037] One of the technical effects of the above-mentioned technical solution is that the width of the liquid channel cannot be too narrow, but is larger than the width of a microscopic imaging image, which facilitates the flow and spreading of the suspension in the cavity.

[0038] One of the technical advantages of the above-mentioned solution is that one sample inlet corresponds to two sample receiving cavities, which facilitates sample injection and improves detection efficiency.

[0039] One of the technical advantages of the above-mentioned solution is that one sample inlet corresponds to multiple sample-containing cavities, and one cavity corresponds to one exhaust channel, which facilitates rapid sample deployment.

[0040] One of the technical effects of the above-mentioned technical solution is that one sample inlet corresponds to multiple sample containing cavities, and one cavity corresponds to one exhaust channel, which reduces the cavity width and prevents cavitation.

[0041] One of the technical effects of the above-mentioned technical solution is that the total area of ​​the microscopic imaging is large enough to facilitate the detection of formed elements of different targets.

[0042] One of the technical advantages of the above-mentioned technical solution is that the sample injection ports are centrally located at the center of the chip, which facilitates sample injection.

[0043] One of the technical effects of the above-mentioned technical solution is that the sample injection port is semi-circular, circular, or elliptical, which facilitates sample injection and also helps the sample to spread in the cavity, thus facilitating microscopic imaging.

[0044] One of the technical advantages of the above-mentioned technical solution is that the area of ​​the sample inlet is larger than that of the exhaust port, which facilitates sample input and gas discharge from the cavity.

[0045] One of the technical advantages of the above-mentioned technical solution is that the multi-layer structure facilitates manufacturing, reduces manufacturing costs, and makes it easier to control processing accuracy.

[0046] One of the technical advantages of the above-mentioned technical solution is that the thickness D of the K2 layer facilitates the design and control of the height of the sample containing cavity.

[0047] One of the technical effects of the above-mentioned technical solution is that the sinking space on the K1 layer and the protrusion on the K2 layer facilitate the height design and control of different sample-containing cavities.

[0048] One of the technical advantages of the above-mentioned technical solution is that the groove design facilitates the height design and control of different sample-accommodating cavities. Attached Figure Description

[0049] Figure 1 This is a schematic diagram of a top perspective view of Embodiment 1; Figure 2 This is a schematic diagram of the top perspective view of detection channel A in Example 1; Figure 3 This is a schematic diagram of a perspective view of Embodiment 1; Figure 4 This is a schematic diagram of the top perspective view of the E detection channel in Example 1; Figure 5 This is a schematic diagram of a top perspective view of Embodiment 2; Figure 6 This is a schematic diagram of a top perspective view of Embodiment 3; Figure 7 This is a schematic diagram of an exploded top perspective view of Embodiment 1; Figure 8 This is a schematic diagram of the top perspective view of Embodiment 4; Figure 9 This is a schematic diagram of the exploded perspective view of Example 4; Figure 10 This is a schematic diagram of an opaque stereoscopic view of Example 5; Figure 11 This is a schematic diagram of a transparent top view of Embodiment 5; Figure 12 This is a schematic diagram of the transparent top view of Embodiment 6; Figure 13 This is a schematic diagram of the transparent top view of Embodiment 7; Figure 14 This is a schematic diagram of the transparent top view of Embodiment 8; Figure 15 This is a schematic diagram of the exploded view of Example 8. Detailed Implementation

[0050] The contents of this application will be further described in detail below with reference to the accompanying drawings.

[0051] It should be noted that the following description of preferred embodiments of this application does not constitute any limitation on this application. The description of preferred embodiments is merely an illustration of the general principles of this application. The embodiments described in this application are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0052] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the equipment or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. Furthermore, the terms "first," "second," and technical features numbered with Arabic numerals 1, 2, 3, etc., and designations such as "A" and "B," are used for descriptive purposes only, for ease of explanation, and do not represent a temporal or spatial order; they should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first," "second," and numbered with Arabic numerals 1, 2, 3, etc., may explicitly or implicitly include one or more of that feature. In the description of this application, "several" means two or more, unless otherwise expressly and specifically defined.

[0053] like Figure 1 A schematic diagram of the top perspective view of Example 1.

[0054] like Figure 1 It includes at least four detection channels: detection channel A 0110, detection channel B 0120, detection channel C 0130, and detection channel D 0140. Each detection channel includes a sample receiving chamber, a sample application port, and an exhaust port.

[0055] like Figure 2 A schematic diagram of the top perspective view of detection channel A in Example 1.

[0056] like Figure 1 The A detection channel includes a sample receiving cavity 0111, a sample application port 0112, and an exhaust port 0113; for example... Figure 2The sample receiving cavity is used to contain liquid samples; the sample receiving cavity includes a microscopic imaging region 0210; the sample inlet is connected to the sample receiving cavity and is used to add liquid samples to the sample receiving cavity; the exhaust port is connected to the sample receiving cavity; the height H of the microscopic imaging region of the sample receiving cavity is greater than 0.1 mm and less than 1.0 mm; the area S of the microscopic imaging region of the sample receiving cavity is greater than 150 square millimeters.

[0057] like Figure 3 A schematic diagram of a three-dimensional view of Example 1.

[0058] like Figure 3 In the opaque state, the sample loading port 0112 and the exhaust port 0113 of the detection channel can be seen on the upper surface of the chip.

[0059] like Figure 1 The heights of the microscopic imaging areas of the sample-containing cavities in detection channels A and B are the same or different; the areas of the microscopic imaging areas of the sample-containing cavities in detection channels A and B are the same or different; the area S of the microscopic imaging area of ​​the sample-containing cavity is less than 500 square millimeters.

[0060] The area of ​​the microscopic imaging region of the sample receiving cavity in detection channel A is AS, and the height is AH; the area of ​​the microscopic imaging region of the sample receiving cavity in detection channel B is BS, and the height is BH; the area of ​​the microscopic imaging region of the sample receiving cavity in detection channel C is CS, and the height is CH; AS, BS, and CS are not equal; and / or AH, BH, and CH are not equal.

[0061] like Figure 1 It also includes a detection channel E.

[0062] like Figure 4 A schematic diagram of the top perspective view of the E detection channel in Example 1.

[0063] like Figure 4 The E detection channel includes a sample receiving chamber 0430, a sample loading port 0410, and an exhaust port 0420.

[0064] like Figure 5 A schematic diagram of the top perspective view of Example 2.

[0065] like Figure 5Example 2 includes two detection channels: Detection Channel A and Detection Channel E. Detection Channel A includes a Sample Receiving Chamber A (0510), a Sample Addition Port A (0513), and an Exhaust Port A (0511). The Sample Addition Port A is connected to the Sample Receiving Chamber A and is used to add liquid sample to the Sample Receiving Chamber A. The Exhaust Port A is also connected to the Sample Receiving Chamber A. Detection Channel E includes a Sample Receiving Chamber E (0520), a Sample Addition Port E (0523), and an Exhaust Port E (0521). The Sample Addition Port E is connected to the Sample Receiving Chamber E and is used to add liquid sample to the Sample Receiving Chamber E. The Exhaust Port E is also connected to the Sample Receiving Chamber E. The width of the microscopic imaging area of ​​the Sample Receiving Chamber in Detection Channel E is smaller than the width of the microscopic imaging area of ​​the Sample Receiving Chamber in Detection Channel A.

[0066] like Figure 5 In blood-related tests, particularly those involving red blood cells, the required sample volume is relatively small. Therefore, the E-channel can be used. The E-channel's sample chamber (0.520) is relatively narrow, requiring only a width greater than the physical width of a single-image photograph. During the test, images are taken in one direction. The A-channel can be used for white blood cell tests. Since the sample preparation methods for these two tests may differ, using two different channels facilitates the testing process.

[0067] This can be defined as follows: For sample A, the height H of the microscopic imaging region can range from greater than 0.1 mm to less than 1.0 mm; the area S of the microscopic imaging region of sample A can be greater than 150 square millimeters. For sample E, the height H of the microscopic imaging region can range from greater than 0.1 mm to less than 1.0 mm; the area of ​​the microscopic imaging region of sample E can be greater than 20 square millimeters and less than 60 square millimeters. Since white blood cells are present in lower concentrations in blood, the sample volume required for white blood cell tests is greater than that required for red blood cell tests.

[0068] The width of the cavity containing sample E is greater than the width of a microscopic image.

[0069] It is possible that the sum of the areas of microscopic imaging region A and microscopic imaging region B is greater than 300 square millimeters.

[0070] like Figure 6 A schematic diagram of a top perspective view of Example 3. It has one more sample detection channel than Example 2.

[0071] like Figure 7 A schematic diagram of an exploded top perspective view of Example 1. Figure 7The chip comprises a transparent cover plate A (0710), an intermediate layer (0720), and a transparent cover plate B (0730), which are bonded together to form a single unit. The hollowed-out portion of the intermediate layer 0720, together with the transparent cover plates A and B, forms a sample receiving cavity, a sample application port, and an exhaust port. In one detection channel, the sample receiving cavity, the sample application port, and the exhaust port are interconnected.

[0072] like Figure 8 The schematic diagram of the top perspective view of Example 4; the chip includes 4 detection channels, of which 1 channel includes a sample receiving cavity 0820, a sample loading port 0830, and an exhaust port 0810.

[0073] like Figure 9 The diagram shows an exploded perspective view of the embodiment. The chip includes a transparent cover plate A 0910 and a transparent cover plate B 0920. Transparent cover plate A includes a recess 0922, and through holes 0921 and 0923. Transparent cover plates A and B are bonded together. The recess 0922 forms a sample receiving cavity, through hole 0921 forms a sample application port, and through hole 0923 forms an exhaust port. The sample receiving cavity, sample application port, and exhaust port are connected to form detection channels. Similarly, the other parts form three detection channels, for a total of four detection channels. Different recess depths can create different sample receiving cavity heights.

[0074] like Figure 10 Example 5: Schematic diagram of an opaque stereoscopic view.

[0075] like Figure 11 Example 5: A schematic diagram of a transparent top view.

[0076] Example 5 includes a detection channel; the detection channel includes at least two sample receiving cavities, a receiving cavity A 1031, a receiving cavity B 1032, a sample application port A 1010, an exhaust port A 1021, and an exhaust port B 1022; the sample receiving cavity A and the sample receiving cavity B are used to receive liquid samples; the sample application port A is connected to the sample receiving cavity A and is used to add liquid samples to the sample receiving cavity A; the exhaust port A is connected to the sample receiving cavity A; the sample application port A is connected to the sample receiving cavity B and is used to add liquid samples to the sample receiving cavity B; the exhaust port B is connected to the sample receiving cavity B; in the sample receiving cavities A and B, the height H of the microscopic imaging area is greater than 0.1 mm and less than 1.0 mm; the sample receiving cavity A includes the microscopic imaging area A; the sample receiving cavity B includes the microscopic imaging area B. Because the cavity is very small, shortening the length and width of the liquid path during the liquid addition process can prevent the formation of air bubbles and reduce the liquid flow length, so that the density of the precipitate is relatively uniform throughout the liquid path.

[0077] like Figure 12Example 6: A schematic diagram of a transparent top view.

[0078] like Figure 12 Compared to Example 5, Example 6 further includes a sample receiving cavity 1210 and an exhaust port 1220; the sample addition port 1210 is connected to the sample receiving cavity, and the sample addition port 1210 is used to add liquid samples to the sample receiving cavity; the exhaust port is connected to the sample receiving cavity.

[0079] like Figure 13 Example 7 is a schematic diagram of a transparent top view. It includes a sample receiving cavity 1320 (D) and an exhaust port 1330 (D); a sample application port 1310 (A) is connected to the sample receiving cavity (D) and is used to add liquid sample to the sample receiving cavity (D); the exhaust port (D) is also connected to the sample receiving cavity (D). Figure 13 Four receiving cavities share a single sample inlet. The cavities can be of the same or different heights to support the same or different detection methods. A circular receiving cavity has a central sample inlet and multiple exhaust ports around its perimeter. Figure 13 This method avoids the formation of air bubbles and is also easy to process.

[0080] like Figure 13 The sample loading ports are centrally located at the center of the chip, while the exhaust ports are located away from the center of the chip.

[0081] The above-mentioned sample inlets are semi-circular or elliptical, as well as square or rectangular.

[0082] The area of ​​the sample inlet is larger than that of the vent, and the vent is smaller, which reduces the volume of ineffective detection liquid.

[0083] like Figure 7 The chip is divided into K1 layer 0710, K2 layer 0720, and K3 layer 0730. The K2 layer is located between the K1 layer and the K3 layer. The K2 layer includes a middle layer cutout, and the K1 layer includes an upper sample loading port cutout and an upper exhaust port cutout. After the K1 layer, K2 layer and K3 layer are bonded together, the middle layer cutout is surrounded by the K1 layer and K3 layer to form a sample receiving cavity. The upper sample loading port cutout is connected to the sample receiving cavity to form a sample loading port. The upper exhaust port cutout is connected to the sample receiving cavity to form an exhaust port.

[0084] Yes, the thickness D of the K2 layer can be greater than 0.1 mm and less than 1.0 mm.

[0085] like Figure 9Layer K1 (0910) or layer K3 (0920) is made of transparent glass or plastic, while layer K2 is made of glass. A recessed space is provided on layer K1, which, together with layer K2, forms a detection channel. Alternatively, a protrusion is provided on layer K2, which, together with the protrusions on layers K1 and K2, forms a detection channel.

[0086] It can include E1 layer and E2 layer; E1 layer and E2 layer are relatively bonded together; E1 layer includes a sample inlet cutout and an exhaust port cutout; the bonding surface of E1 layer or E2 layer includes a groove; after E1 layer and E2 layer are bonded together, the groove is surrounded by E1 layer and E2 layer to form a sample receiving cavity; the sample inlet cutout is connected to the sample receiving cavity to form a sample inlet; the exhaust port cutout is connected to the sample receiving cavity to form an exhaust port.

[0087] It is permissible that the depth of at least one groove is different from the depth of the other grooves.

[0088] like Figure 14 Example 8: A schematic diagram of a transparent top view.

[0089] Example 8 includes four detection channels: detection channel A, detection channel B, detection channel C, and detection channel D.

[0090] The detection channel A includes an A1 receiving chamber, an A2 receiving chamber, a sample application port, and an exhaust port.

[0091] The B detection channel includes a receiving chamber, a sample inlet, and an exhaust outlet.

[0092] The C detection channel includes a receiving chamber, a sample inlet, and an exhaust port.

[0093] The area of ​​the cavity for detection channel B is the same as that for detection channel C, but the height of the cavity can be the same or different.

[0094] The D-channel detection system includes a receiving chamber, a sample inlet, and an exhaust port. The length of the receiving chamber in the D-channel detection system is significantly greater than its width.

[0095] like Figure 15 The diagram shows an exploded view of Embodiment 8. Embodiment 8 is composed of a transparent cover plate 1610, a hollowed-out intermediate layer 1620, and a transparent cover plate 1630 bonded together.

[0096] In some embodiments, the transparent cover plate in this application may be partially transparent, with the middle layer being a non-transparent material and the middle layer being transparent.

[0097] While this application has been described and illustrated with reference to preferred embodiments and several alternatives, it is not intended to be limited to the specific descriptions herein. Other alternatives or equivalent components may also be used to practice this application.

Claims

1. A formed element detection chip with a multi-sample accommodating cavity, characterized in that, Includes at least four testing channels; The detection channel includes a sample receiving cavity, a sample application port, and an exhaust port; The sample receiving cavity is used to contain liquid samples; the sample receiving cavity includes a microscopic imaging area; The sample inlet is connected to the sample receiving cavity and is used to add liquid sample to the sample receiving cavity; the exhaust port is also connected to the sample receiving cavity. The height H of the microscopic imaging region of the sample containing cavity is greater than 0.1 mm and less than 1.0 mm; the area S of the microscopic imaging region of the sample containing cavity is greater than 150 square millimeters.

2. The formed element detection chip with a multi-sample accommodating cavity according to claim 1, characterized in that, The testing channels include testing channel A and testing channel B; The height of the microscopic imaging area of ​​the sample containing cavity in detection channel A and detection channel B may be the same or different. The areas of the microscopic imaging regions of the sample-containing cavities in detection channels A and B may be the same or different. The area S of the microscopic imaging region of the sample containing cavity is less than 500 square millimeters.

3. The formed element detection chip with a multi-sample accommodating cavity according to claim 1, characterized in that, The detection channels include detection channel A, detection channel B, detection channel C, and detection channel D; The area of ​​the microscopic imaging region of the sample-containing cavity in detection channel A is AS, and the height is AH. The area of ​​the microscopic imaging region of the sample-containing cavity in detection channel B is BS, and the height is BH. The area of ​​the microscopic imaging region of the sample-containing cavity in the C detection channel is CS, and the height is CH. AS, BS, and CS are not equal, and / or AH, BH, and CH are not equal.

4. A formed element detection chip, characterized in that, It includes at least two detection channels, detection channel A and detection channel E; Detection channel A includes sample receiving chamber A, sample application port A, and exhaust port A; Sample inlet A is connected to sample receiving chamber A, and is used to add liquid sample to sample receiving chamber A; vent outlet A is connected to sample receiving chamber A. The E detection channel includes an E sample receiving chamber, an E sample application port, and an E exhaust port; The E sample inlet is connected to the E sample receiving cavity, and the E sample inlet is used to add liquid sample to the E sample receiving cavity; the E exhaust port is also connected to the E sample receiving cavity. The width of the microscopic imaging area of ​​the sample receiving cavity in detection channel E is smaller than the width of the microscopic imaging area of ​​the sample receiving cavity in detection channel A.

5. The formed element detection chip according to claim 4, characterized in that, The height H of the microscopic imaging region of the sample-containing cavity is greater than 0.1 mm and less than 1.0 mm; the area S of the microscopic imaging region of the sample-containing cavity is greater than 150 square millimeters. The height H of the microscopic imaging area of ​​the E sample containing cavity is greater than 0.1 mm and less than 1.0 mm; the area of ​​the microscopic imaging area of ​​the E sample containing cavity is greater than 20 square millimeters and less than 60 square millimeters.

6. The formed element detection chip according to claim 4, characterized in that, The width of the cavity containing the E sample is greater than the width of a microscopic image.

7. A formed element detection chip with a multi-sample accommodating cavity, characterized in that, It includes at least one detection channel; the detection channel includes at least two sample receiving cavities, at least one sample application port, and at least two exhaust ports; The sample receiving cavity includes sample receiving cavity A and sample receiving cavity B; The sample inlet includes sample inlet A; the exhaust outlet includes exhaust outlet A and exhaust outlet B. Sample A and Sample B are used to contain liquid samples; Sample inlet A is connected to sample receiving chamber A, and is used to add liquid sample to sample receiving chamber A; vent outlet A is connected to sample receiving chamber A. Sample inlet A is connected to sample receiving chamber B, and is used to add liquid sample to sample receiving chamber B; vent outlet B is connected to sample receiving chamber B. In the sample A and sample B accommodating cavities, the height H of the microscopic imaging area is greater than 0.1 mm and less than 1.0 mm. Sample A's cavity includes microscopic imaging region A; Sample B's cavity includes microscopic imaging region B.

8. The formed element detection chip with a multi-sample accommodating cavity according to claim 7, characterized in that, The combined area of ​​the microscopic imaging region A and the microscopic imaging region B is greater than 300 square millimeters.

9. The formed element detection chip with a multi-sample accommodating cavity according to claim 7, characterized in that, It also includes a C sample receiving cavity and a C exhaust port; Sample inlet A is connected to sample receiving chamber C, and is used to add liquid sample to sample receiving chamber C; vent outlet C is connected to sample receiving chamber C. And / or also includes a sample receiving cavity D and an exhaust port D; a sample application port A is connected to the sample receiving cavity D, and the sample application port A is used to add liquid sample to the sample receiving cavity D; the exhaust port D is connected to the sample receiving cavity D.

10. The formed element detection chip with a multi-sample accommodating cavity according to any one of claims 1 to 9, characterized in that, The sample loading ports are centrally located at the center of the chip, while the exhaust ports are located away from the center of the chip.

11. The formed element detection chip with a multi-sample accommodating cavity according to any one of claims 1 to 9, characterized in that, The sample dispensing port is semi-circular or elliptical.

12. The formed element detection chip with a multi-sample accommodating cavity according to any one of claims 1 to 9, characterized in that, The area of ​​the sample inlet is larger than that of the exhaust port.

13. The formed element detection chip with a multi-sample accommodating cavity according to any one of claims 1 to 9, characterized in that, Including K1 layering, K2 layering, and K3 layering; Layer K2 is located between layers K1 and K3. Layer K2 includes a hollowed-out middle layer, while layer K1 includes a hollowed-out upper sample inlet and a hollowed-out upper exhaust outlet. After K1 layer, K2 layer and K3 layer are bonded together, the hollow part of the middle layer is enclosed by K1 layer and K3 layer to form a sample receiving cavity. The upper sample dispensing port is connected to the sample receiving cavity to form a sample dispensing port; The upper exhaust port is connected to the sample receiving cavity to form an exhaust port.

14. The formed element detection chip with a multi-sample accommodating cavity according to claim 13, characterized in that, The thickness D of the K2 layer is greater than 0.1 mm and less than 1.0 mm.

15. The formed element detection chip with a multi-sample accommodating cavity according to claim 13, characterized in that, The K1 layer or K3 layer is made of transparent glass or plastic, and the K2 layer is made of glass; A sunken space is set on layer K1, and the sunken space on layer K1 and layer K2 enclose and form a detection channel; Alternatively, protrusions can be set on the K2 layer, and the protrusions on the K1 and K2 layers can be combined to form a detection channel.

16. The formed element detection chip with a multi-sample accommodating cavity according to any one of claims 1 to 9, characterized in that, It includes E1 layer and E2 layer; E1 layer and E2 layer are relatively attached to each other as one; E1 layer includes a sample inlet cutout and an exhaust outlet cutout. On the E1 or E2 layer bonding surface, there is a groove; after the E1 layer and E2 layer are bonded together, the groove is surrounded by the E1 layer and E2 layer to form a sample receiving cavity. The sample dispensing port is connected to the sample receiving cavity to form a sample dispensing port; The vent opening is connected to the sample receiving cavity to form an vent.

17. The formed element detection chip with a multi-sample accommodating cavity according to claim 16, characterized in that, The depth of at least one groove is different from the depth of the other grooves.

Images