Seed sample positioning device and non-destructive micro-measurement system

Abstract

The utility model discloses a seed sample positioning device and non -invasive micro -measuring system relates to non -invasive micro -measuring technical field. Seed sample positioning device includes the positioning plate, is equipped with seed positioning groove and intercommunication groove on the positioning plate. The number of seed positioning groove is multiple, is used for positioning seed sample. The both ends of intercommunication groove are connected two seed positioning grooves respectively, and the sensor of non -invasive micro -measuring system moves through. Compared with prior art, the sensor in the utility model detects the seed in one seed positioning groove, and after finishing, need not to stretch out liquid level, only need to move to another seed positioning groove along intercommunication groove, omitted the operation of sensor stretching out liquid level, improved the detection efficiency of batch seed sample, avoided the influence of potential change because of frequently going in and out liquid level to the detection result.

Description

Technical Field

[0001] This utility model relates to the field of non-destructive micro-measurement technology, and in particular to a seed sample positioning device and a non-destructive micro-measurement system. Background Technology

[0002] In recent years, non-invasive micro-test technology (NMT) has attracted widespread attention due to its advantages of being label-free, real-time dynamic, and enabling in-situ detection. This technology captures transmembrane flow information of molecules or ions on the sample surface using sensors, avoiding damage to the sample.

[0003] However, existing non-destructive microseed testing systems are mainly designed for single-sample testing and rely on manual operation, which makes it impossible to achieve fully automated, high-throughput seed population analysis, thus limiting their application in modern agricultural research and breeding.

[0004] Although standard laboratory plates on the market have multiple wells, these wells are independent of each other. When the sensor detects samples in different wells, it needs to frequently enter and exit the liquid surface, which leads to excessive potential changes and affects the detection results. Utility Model Content

[0005] The purpose of this invention is to provide a seed sample positioning device and a non-destructive micro-measurement system to solve the problems existing in the above-mentioned related technologies and avoid frequent entry and exit of the sensor from the liquid surface.

[0006] To achieve the above objectives, this utility model provides the following solution: This utility model discloses a seed sample positioning device, including a positioning plate, wherein the positioning plate is provided with seed positioning grooves and connecting grooves; the number of seed positioning grooves is multiple, used for positioning seed samples; the two ends of the connecting grooves are respectively connected to two seed positioning grooves, allowing the sensors of a non-destructive micro-measurement system to move through.

[0007] In some examples, all of the seed positioning slots are directly or indirectly connected to each other through the connecting slots.

[0008] In some examples, the plurality of seed positioning slots are distributed in a rectangular array, and two adjacent seed positioning slots along the length and width of the rectangular array are connected by the connecting slot.

[0009] In some examples, the seed positioning groove is a spherical groove.

[0010] In some examples, the depth of the spherical groove is less than the radius of the spherical groove.

[0011] In some examples, the depth of the spherical groove is not less than 8 mm.

[0012] In some examples, the diameter of the spherical groove is not less than 18 mm.

[0013] In some examples, the depth and width of the connecting groove are not less than 4 mm.

[0014] In some examples, the positioning plate is a rectangular plate, and transition structures are provided at the four corners of the positioning plate.

[0015] This utility model also discloses a non-destructive micro-measurement system, including the above-mentioned seed sample positioning device.

[0016] This utility model achieves the following technical advantages compared to related technologies: Non-destructive microseam systems capture transmembrane flow information of molecules or ions on the sample surface using sensors, avoiding sample damage. When testing a batch of seeds, existing non-destructive microseam systems typically use a standard experimental plate to position the seeds, placing each seed individually in its corresponding well and immersing it in liquid. The sensor then moves to the vicinity of each seed to collect transmembrane flow information. Because the wells of the standard experimental plate are independent, the sensor needs to frequently enter and exit the liquid surface when detecting seeds in different wells, leading to excessive potential changes and affecting the detection results.

[0017] This invention utilizes a connecting groove to link two seed positioning grooves. After the sensor detects a seed in one groove, it can move along the connecting groove to the other groove to detect the next type of seed without needing to extend it above the liquid surface. This process eliminates the need for the sensor to extend above the liquid surface, improving the detection efficiency of batch seed samples and avoiding the impact of potential changes caused by frequent entry and exit from the liquid surface on the detection results.

[0018] In a preferred embodiment of this invention, all seed positioning slots are directly or indirectly connected to each other via connecting slots. Since the connecting slots can guide the liquid flow, it is unnecessary to add liquid to each seed positioning slot individually during operation; liquid only needs to be added to one seed positioning slot, and the liquid will be diverted to all seed positioning slots via the connecting slots, improving work efficiency. Furthermore, because all seed positioning slots are directly or indirectly connected to each other via connecting slots, the liquid level in each seed positioning slot is consistent, ensuring a consistent testing environment. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or related technologies, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of a seed sample positioning device in some examples of this utility model; Figure 2 for Figure 1 Top view of the structure; Figure 3 for Figure 1 A sectional view of the middle structure; Figure 4 for Figure 1 Side view of the structure.

[0021] In the diagram: 1-positioning plate; 2-seed positioning groove; 3-connecting groove; 4-transition structure. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] The purpose of this invention is to provide a seed sample positioning device and a non-destructive micro-measurement system to solve the problems existing in the above-mentioned related technologies and avoid frequent entry and exit of the sensor from the liquid surface.

[0024] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0025] Reference Figures 1-4 This embodiment provides a seed sample positioning device, including a positioning plate 1, on which seed positioning grooves 2 and connecting grooves 3 are provided. Multiple seed positioning grooves 2 are provided for positioning seed samples. The connecting grooves 3 are connected to two seed positioning grooves 2 at each end, allowing sensors from a non-destructive microelectrode system to move through.

[0026] The working principle of the seed sample positioning device in this embodiment is as follows: Non-destructive microseam systems capture transmembrane flow information of molecules or ions on the sample surface using sensors, avoiding sample damage. When testing a batch of seeds, existing non-destructive microseam systems typically use a standard experimental plate to position the seeds, placing each seed individually in its corresponding well and immersing it in liquid. The sensor (its detection end) moves to the vicinity of each seed to collect transmembrane flow information. Because the wells of the standard experimental plate are independent, the sensor needs to frequently enter and exit the liquid surface when detecting seeds in different wells, leading to excessive potential changes and affecting the detection results.

[0027] In this embodiment, a connecting groove 3 is provided to connect two seed positioning grooves 2. After the sensor has detected a seed in one seed positioning groove 2, it does not need to extend out of the liquid surface. It can simply move along the path of the connecting groove 3 to another seed positioning groove 2 to detect the other type of seed. The above process omits the operation of the sensor extending out of the liquid surface, which not only improves the detection efficiency of batch seed samples, but also avoids the influence of potential changes caused by frequent entry and exit from the liquid surface on the detection results.

[0028] The process of the sensor moving along the path of the connecting groove 3 to another sub-positioning groove 2 can include only a horizontal movement path, or a combination of a horizontal and a vertical movement path, as long as the sensor's detection end remains below the liquid surface. When the movement path only includes a horizontal movement path, the detection efficiency is higher.

[0029] It should be noted that in the operation of the non-destructive microelectrode system, the sensor has a working electrode and a reference electrode. The working electrode directly contacts the detection area near the sample, and its potential changes in real time with the concentration of the target ion; the higher the concentration, the higher the potential (following the Nernst equation). In existing technologies, the connecting groove 3 is not provided, and the seed positioning holes are not connected. Therefore, the reference electrode needs to frequently enter and exit the solution along with the working electrode, which affects the potential. Furthermore, the diameter of the reference electrode is generally greater than 2 mm. When it enters the smaller seed positioning hole containing the sample, without manual adjustment or relevant equipment functions, it is highly likely to collide with the sample, affecting the experiment. In this embodiment, the connecting groove 3 connects the various seed positioning holes 2. The reference electrode is inserted into a seed positioning hole filled only with liquid and without seeds, and its potential remains stable. There is no issue of the reference electrode colliding with the sample due to frequent entry and exit from the liquid surface. The working electrode of the sensor moves along the connecting groove 3, while the reference electrode remains stationary. By measuring the potential difference between the working electrode and the reference electrode, detection errors caused by environmental interference can be avoided, and the potential signal caused by the difference in target ion concentration can be accurately measured.

[0030] In some examples, all seed positioning slots 2 are directly or indirectly connected to each other via connecting slots 3.

[0031] Since the connecting channel 3 can guide the liquid flow, it is not necessary to add liquid to each seed positioning channel 2 separately during operation. Liquid only needs to be added to one of the seed positioning channels 2, and the liquid can be diverted from the connecting channel 3 to all the seed positioning channels 2, which improves work efficiency.

[0032] Furthermore, since all seed positioning slots 2 are directly or indirectly connected to each other through connecting slots 3, the liquid level in each seed positioning slot 2 is consistent, ensuring the consistency of the detection environment.

[0033] After the reference electrode is fixed in any seed positioning slot 2, the potential of the entire positioning plate 1 is closed loop, and there is no need to frequently move the position of the reference electrode.

[0034] In some examples, multiple seed positioning slots 2 are distributed in a rectangular array, and two adjacent seed positioning slots 2 along the length and width directions of the rectangular array are connected by the connecting slots 3.

[0035] This setup makes it easy to distinguish seeds by row and column number and count the total number. The horizontal position of the sensor can be adjusted using a drive device such as a cross slide that can move the sensor in mutually perpendicular directions.

[0036] Depending on the actual needs, other configurations of the connecting slot 3 can also be selected, such as extending the connecting slot 3 along the diagonal parallel to the rectangular array.

[0037] For example, the row spacing and column spacing of the rectangular array are the same (in this embodiment, the spacing refers to the center distance).

[0038] For example, the row spacing and column spacing of the rectangular array are both 24mm.

[0039] However, the actual implementation is not limited to this. For example, the multiple seed positioning slots 2 can also be arranged in a circular array. In this case, the horizontal position of the sensor can be adjusted by a rotation drive device to change the circumferential position of the sensor.

[0040] In some examples, the seed positioning slot 2 is a spherical slot.

[0041] The middle part of the spherical groove is deeper than the edge of the spherical groove. When a seed is placed in the spherical groove, it rolls or slides under its own weight to keep its position at the bottom of the spherical groove, thus achieving accurate positioning of the seed.

[0042] In some examples, the depth of the spherical groove is less than the radius of the spherical groove.

[0043] That is, the opening of the spherical groove is located at the position with the largest cross-sectional area, which facilitates the placement and removal of seeds from the spherical groove, as well as the subsequent cleaning of the seed sample positioning device, and avoids the constricted groove opening from obstructing the operation.

[0044] In some examples, the depth of the spherical groove is not less than 8 mm.

[0045] This depth range is greater than the diameter of most soybean, rice, wheat, and other seeds, allowing the liquid in the spherical trough to submerge the seeds when they are placed flat in it.

[0046] It should be noted that the seed sample positioning device in this embodiment is not only suitable for small-sized seeds such as Arabidopsis thaliana, tobacco, and rapeseed, but also for medium-sized seeds such as soybeans, rice, and wheat, as well as larger-sized seeds such as peanuts and lotus seeds.

[0047] For example, the depth of the spherical groove is 9 mm.

[0048] In some examples, the diameter of the spherical groove is not less than 18 mm.

[0049] For example, the diameter of the spherical groove is 20 mm.

[0050] In some examples, the depth and width of the connecting slot 3 are not less than 4 mm.

[0051] For example, the depth and width of the connecting slot 3 are both 5mm.

[0052] In some examples, the positioning plate 1 is a rectangular plate, and transition structures 4 are set at the four corners of the positioning plate 1.

[0053] The transition structure 4 at the four corners of the positioning plate 1 can be either a rounded transition or a chamfered transition.

[0054] For example, the radius of the rounded corner transition is 2mm.

[0055] For example, the rectangular plate is a square plate with a side length of 173mm, and the number of rows and columns of the seed positioning groove 2 on one side of the plate is 7.

[0056] For example, the long side of the rectangular plate is parallel to the long side of the rectangular array, and the wide side of the rectangular plate is parallel to the wide side of the rectangular array.

[0057] For example, the distance between the long side of the rectangular plate and the center of its adjacent seed positioning groove is 24mm, and the distance between the wide side of the rectangular plate and the center of its adjacent seed positioning groove is 24mm.

[0058] In some examples, the seed sample positioning device is molded in a mold by injection molding. This molding method directly produces a seed sample positioning device with seed positioning grooves and connecting grooves, eliminating the need for grooving operations, improving processing efficiency, and reducing processing costs.

[0059] In some examples, the seed sample positioning device also includes a cover plate for covering one side of the positioning plate 1 where the seed positioning slots 2 and connecting slots 3 are provided, so as to prevent dust from entering the seed positioning slots 2 and connecting slots 3 when the experiment is completed and the samples are stored.

[0060] In some examples, the positioning plate 1 can be made of glass, resin, plastic, etc., i.e., transparent or translucent materials.

[0061] In some examples, the seed sample positioning device also includes a cleaning brush, the width of which is no greater than the width of the connecting groove 3, for cleaning the connecting groove 3. The brush head can be made of nylon to avoid scratching the positioning plate 1. Depending on the actual needs, those skilled in the art may also choose brush heads made of other materials.

[0062] This embodiment also provides a non-destructive micro-measurement system, including the seed sample positioning device described above.

[0063] Since the non-destructive micro-measurement system includes the aforementioned seed sample positioning device, it also possesses the corresponding advantages of the seed sample positioning device, which will not be elaborated here.

[0064] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A seed sample positioning device, characterized in that: The system includes a positioning plate (1), which has a seed positioning groove (2) and a connecting groove (3). There are multiple seed positioning grooves (2) for positioning seed samples. The two ends of the connecting groove (3) are respectively connected to two seed positioning grooves (2) for the sensor of the non-destructive micro-measurement system to move through.

2. The seed sample positioning device according to claim 1, characterized in that: All the seed positioning slots (2) are directly or indirectly connected to each other through the connecting slots (3).

3. The seed sample positioning device according to claim 2, characterized in that: The seed positioning slots (2) are arranged in a rectangular array, and two adjacent seed positioning slots (2) along the length and width of the rectangular array are connected by the connecting slot (3).

4. The seed sample positioning device according to claim 1, characterized in that: The seed positioning groove (2) is a spherical groove.

5. The seed sample positioning device according to claim 4, characterized in that: The depth of the spherical groove is less than the radius of the spherical groove.

6. The seed sample positioning device according to claim 5, characterized in that: The depth of the spherical groove is not less than 8 mm.

7. The seed sample positioning device according to claim 6, characterized in that: The diameter of the spherical groove is not less than 18 mm.

8. The seed sample positioning device according to claim 1, characterized in that: The depth and width of the connecting groove (3) are not less than 4 mm.

9. The seed sample positioning device according to claim 1, characterized in that: The positioning plate (1) is a rectangular plate, and transition structures (4) are provided at the four corners of the positioning plate (1).

10. A non-destructive micro-probe system, characterized in that: Includes the seed sample positioning device as described in any one of claims 1 to 9.

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